[Histonet] L.A.B. Solution
dteleis <@t> vet.upenn.edu
Sat Dec 13 06:17:35 CST 2003
We used the LAB solution recently for IGF-1, and it worked well at 10 min, RT.
Thetissue was fixed in formalin and embedded in both parafin and MMA. The MMA
sections look like they may benefit from longer exposure, but we did have some
>===== Original Message From <kwuny <@t> email.cs.nsw.gov.au> =====
>Has anyone ever tried Polysciences' Liberate Antibody Binding (LAB)
>Solution? The product (www.polysciences.com Cat.No. 24310) is supposed to
>exposure the antigenic site of the FFPE tissue sections by incubating at
>room temperature for 5-20 minutes, eliminating the used of heating (HIER)!!
>This would give enormous changes the way we do immunostaining so far.
>Among few antibodies I tried so far, only LCA (CD45) and CD20 (L-26) were
>good enough and Progesterone receptor showed a weak staining.
>Senior Hospital Scientist
>Dept. of Anatomical Pathology
>Concord NSW 2139 Australia
>kwuny <@t> email.cs.nsw.gov.au
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