[Histonet] L.A.B. Solution

[Donna Teleis]

We used the LAB solution recently for IGF-1, and it worked well at 10 min, RT. 
Thetissue was fixed in formalin and embedded in both parafin and MMA. The MMA 
sections look like they may benefit from longer exposure, but we did have some 
staining.





>===== Original Message From <kwuny <@t> email.cs.nsw.gov.au> =====
>Hi Histonetters,
>Has anyone ever tried Polysciences' Liberate Antibody Binding (LAB)
>Solution?  The product (www.polysciences.com Cat.No. 24310) is supposed to
>exposure the antigenic site of the FFPE tissue sections by incubating at
>room temperature for 5-20 minutes, eliminating the used of heating (HIER)!!
>This would give enormous changes the way we do immunostaining so far.
>
>Among few antibodies I tried so far, only LCA (CD45) and CD20 (L-26) were
>good enough and Progesterone receptor showed a weak staining.
>
>
>Regards
>
>
>
>Young Kwun
>Senior Hospital Scientist
>Dept. of Anatomical Pathology
>Concord Hospital
>Concord NSW 2139 Australia
>Tel)61-2-9767-6075
>Fax)61-2-9767-8427
>kwuny <@t> email.cs.nsw.gov.au
>
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Donna Teleis
Research Specialist
Department of Clinical Studies
Equine Sports Medicine Building
University of Pa., New Bolton Center
phone: 610-925-6429 
fax: 610-925-8131