[Histonet] polymerization problems
Barry R Rittman
Barry.R.Rittman <@t> uth.tmc.edu
Fri Dec 12 08:25:57 CST 2003
First is that with mineralized tissues there will be a tendency for some
regions with high mineral content to act as focal points for
polymerization to start and consequently this often results in bubbles
starting at that point.
The second is that I have often wondered if thick solutions of
methacrylate and other resins should be subjected to ultrasonic
treatment before use. We used this method when mixing Epon and other
resins for electron microscopy and it virtually eliminated bubble
formation around tissues. Just a thought.
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of louise
Sent: Friday, December 12, 2003 4:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] polymerization problems
Well, this is my 2 cents (South African) worth:
1. I have, as yet, not been able to get the stuff to polymerise at cold
temperatures. I have had very good sucess at RT, although with such
tissues, trial and error as well as judicious advice from fellow
histonetters, it is recommended that the specimen is "layered" with
embedding solution and allowed to polymerise over a few days. Otherwise
unsightly and potentially bubbly and uncutable blocks result.
2. I have found that, because the 2 embedding solutions are so viscous
they must be well mixed before use. I generally stir mine for about 20
minutes on a magnetic stirrer before pouring into the mould. (do this in
3. Heraeus Kulzer have a very nice technical assistant in Germany who
been very helpful in the past. If all else fails get hold of her for
further insights. She is Ulrike Leins at: Ulrike.Leins <@t> t-online.de
Bone Research Unit
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"
----Original Message Follows----
From: "Tom Schaer" <tpschaer <@t> vet.upenn.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] polymerization problems
Date: Wed, 10 Dec 2003 09:32:05 -0500
I am going to try again, realizing that not many have vast experience
embedding large specimen with technovit 9100 yet. Maybe someone may be
to share some pointers for a non-profit lab?
Essentially, we fail to successfully polymerize med fem ovine condyles
approx 100ml jars. Tried at -15C with no change in solution consistency
a week; then brought it up to -2C still no change over three days. Jars
were "vacuumed" and set in refrigerator / freezer. I am at the of my
Thanks for any pointers.
Thomas P. Schaer, VMD
Assist. Prof. BIOMED, Drexel University
Comparative Orthopaedic Research & Tissue Engineering Department of
Clinical Studies University of Pennsylvania New Bolton Center 382 West
Street Road Kennett Square, PA 19348 tel. 610-444-5800 (x6261 office)
tel. 610-444-5800 (x6131 lab) fax. 610-925-8100
tpschaer <@t> mail.vet.upenn.edu
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