[Histonet] Re: Histonet digest, Vol 1 #167 - 24 msgs

[tony.j.savage <@t> gsk.com]

Nancy,
      In response to message 23 (Vol1 #167 - 24), Why don't you stain with
alcian blue / PAS before you apply your emulsion. Both alcian blue and PAS
should survive subsequent development. I have used this technique in the
past for some of my autoradiographs. You would obviously have to assess
whether or not you are compromising your in situ signal. However I would
have thought you would be on safe ground if you just stained with alcian
blue for a couple of minutes.
                                        Regards,
                                                    Tony
Histopathology Group
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