[Histonet] working with Vectashield with propidium Iodide

[Andrew Lust]

Dear histonetters

Just wanted to know if there is anything I need to do when handling 
Vectashield with Propidium Iodide?  Sometimes the Vectashield propidium 
iodide works and sometimes it doesn't.  Can anyone tell me what I need to do 
step by step so that i can get consistent results with vectashield PI.

do you allow the section to dry before placing the vectashield (PI) then 
placing the cover slip.
Or do you wash then wipe around the section and then place Vectashield with 
PI on the tissue then coverslip?

How sensitive is Vectashield PI to light.

andrew

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