[Histonet] Cox-2 antibody from Santa Cruz
Bonnie P Whitaker
Bonnie.P.Whitaker <@t> uth.tmc.edu
Wed Dec 10 10:42:09 CST 2003
Are you getting any staining? (Please describe exactly what the problem
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Victoria
Sent: Wednesday, December 10, 2003 10:21 AM
To: HistoNet Server
Subject: [Histonet] Cox-2 antibody from Santa Cruz
Is anyone currently using the Cox-2 (goat
polyclonal)from Santa Cruz? Up until my last two runs
I wasn't having any problems with it.
My concentration is 1:1000, I'm using EDTA as HIER in
a microwave (1200watt, two 3 minute cycles pl 7;
boil/simmer is approximately 30 seconds each). I'm
using a Vector universal ABC detection kit with Nova
Red chromogen for visualization. The tissue is human
cervical samples, and I've had good results from them
in the past.
I have replaced the antibody and the chromogen. The
detection kit is only 3 months old.
Any input would be very much appreciated.
Institute for Cancer Prevention
Valhalla, NY 10595
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