[Histonet] IHC on coverglasses.

Ian Montgomery ian.montgomery <@t> bio.gla.ac.uk
Wed Dec 10 10:03:45 CST 2003

         Put a small spot of silicone grease in the middle of a glass 
slide. Drain the coverglass then touch against the grease, cells up, press 
gently then ring with a pen. Handle with care during the technique and 
press again if you think it's needed. When you're finished simply slip off 
and mount on a slide as usual. If aqueous I clean the excess grease off 
after the mountant has set. If you dehydrate the grease dissolves in the 
clearing agent. Vaseline works but silicone grease gives a better 
attachment during all the rinsing steps.

Dr. Ian Montgomery,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 

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