[Histonet] collagen

[Patsy Ruegg]

Megan,
I would first do a stain for proteoglycan, something like 0.2% Azure B ph 6
to see what is there.  Just stain with this for 5 min., rinse in dih20
airdry and coverslip.  This will demonstrate everything from collagen to
cartilage to bone formation and is very simple.  If you want to get to
classifying the collagen type I recommend using the antibodies from U of
Iowa Hybridoma Bank, they have all kinds of collagens.
Collagen antibodies can be obtained from Developmental Studies Hybridoma
Bank, Department of Biological Sciences, University of Iowa, 007 BBE, IowA
City, IA 52242-1324  tele: 319-335-3826, fax 319-335-2077 email
dshb <@t> uiowa.edu
Patsy
-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Megan Kear
Sent: Sunday, December 07, 2003 6:13 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] collagen

Hi ,
could someone help with a recommendation for staining "newly laid
colagen",we think it is type v but not quite sure.Any suggestions
would
be of great help.
Thank You
Megan Kear
HAPS
Newcastle
Australia


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