[Histonet] RE: cryosectioning adult fish teeth?

Josh Trapani trapani <@t> spot.colorado.edu
Mon Dec 8 10:50:07 CST 2003

Dear All,

I would like to thank everyone who replied to my request last week for
some advice on cryosectioning pharyngeal teeth of adult fish. If you are
curious as to the responses, I had at least three people recommend using
tape-transfer methods. These are new to me, and I'm starting to do some
research on them. Someone else also recommended a modified
freezing/cutting procedure; in particular, freezing the specimen slowly
(little by little, that is) in a rectangular mold and then positioning the
edge of the mold parallel to the blade (I had been doing quick - all at
once - freezing in circular molds). I haven't tried this yet, but will
within in a few days when I get back on the machine.

Again, thank you for your help.

Josh Trapani
University of Colorado

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