[Histonet] water purification

Monson, Frederick fmonson <@t> wcupa.edu
Mon Dec 8 07:55:03 CST 2003

Morning Evelyn,
	There should be NO conductivity and resistance, therefore,
should be EXTREMELY high.  For all molecular biology procedures that I
can remember using, I redistilled deionized water in a scrupulously
clean all glass still.  Once I had a system, I used such water for all
my histo- or cyto- preparations and methods, and reserved tap water for
post-rinsing Schiff's reagent and final stabilization of H in H&E.

	With respect to deionizers, the ion trapping bed is generally
specially chosen for the water that is to be processed, and thus, often
does a more-than-adequate job.


Fred Monson

 Frederick C. Monson, PhD
Center for Advanced Scientific Imaging
Mail to Geology
West Chester University of Pennsylvania
Schmucker II Science Center, Room SS024
South Church Street and Rosedale Avenue
West Chester, PA, 19383
Phone/FAX:  610-738-0437
eMail:  fmonson <@t> wcupa.edu
CASI Page and Scheduling

-----Original Message-----
From: Evelyn Kaplan [mailto:ekaplan <@t> squ.edu.om] 
Sent: Monday, December 08, 2003 5:29 AM
To: Histonet
Subject: [Histonet] water purification

Good afternoon,

I wonder if someone could tell me;
What sort of conductivity should one obtain in water purification
systems (deinoised water)for use in PCR? I would be much obliged if you
could help out.


Evelyn Kaplan,
Dept of Pathology,
College of Medicine and Health Sciences,
Sultan Qaboos University,

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