[Histonet] Hardening very soft brains

Geoff McAuliffe mcauliff <@t> umdnj.edu
Fri Dec 5 09:22:20 CST 2003

Hi Steve:

How long are you fixing the brains? 48 hours is the minimum for 
formalin, no matter what the concentration. One week is not too long.
How big are the brains? Fetal mose brain is easier to fix by immersion 
than a fetal horse brain. Size does matter.
Are you sure your formalin is good?


Steve Machin UK wrote:

>Could anyone help us with a problem we have in processing very soft
>fetal brains?
>We currently fix in 20% formalin in buffer but the brains are so soft
>after processing they stick to the wrapping paper.
>Any ideas on how we can harden them after fixation?
>Best Wishes
>Steve Machinu UK
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu

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