[Histonet] Stain for H.Pylori

[rfail]

Peggy,
    There is a slightly different Giemsa method in
Histologic for H. pyloric using a microwave.
Rena Fail


 How does one make up the "stock" Giemsa, which is then
> diluted to make the working?  - concentration? (X grams
> Giemsa powder to Y grams solvent?) - pH? (with what -
> acetic acid? buffer? Which one?) - aqueous or alcoholic?
> - if alcoholic, what %?? which alcohol? (ethanol?
> methanol?) - etc.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
>   ----- Original Message -----
>   From: Hernan Aldana Marcos
>   To: Jill Cox
>   Cc: histonet <@t> lists.utsouthwestern.edu
>   Sent: Wednesday, December 03, 2003 1:20 PM
>   Subject: Re: [Histonet] Stain for H.Pylori
>
>
>   MODIFICATION OF GIEMSA STAIN FOR HELICOBACTER
>
>   FIXATION: Neutral Formolin   10%
>
>   SECCIONS: 4-5 µm
>
>
>
>   SOLUTIONS:
>
>   ACETIC WATER
>
>   Acetic acid............... 0,01 ml
>
>   Destilated water............... 1000,00 ml
>
>
>
>   Working solution GIEMSA
>
>   Giemsa.............. 10,0 ml
>
>   Acetic water.............. 50,0 ml
>
>
>
>   PROCEDDURE
>
>   1.     Take slides to water destiled
>
>   2.     Giemsa working solution 1 h
>
>   3.     Deshidratation in 3 changes of alcohol 100
>
>   4.     xilol, syntetic mounting medium
>
>
>
>   RESULTADOS
>
>   H. pylorii.............. intense blue
>
>   Nuclei.............. blue
>
>   Cytoplasm.............. Rose
>
>
>
>   REFERENCE
>
>   Luna L.: "Wet work shop for special Alabama" Noviembre 2
> de 1984
>
>
>   Dr. Hernán J. Aldana Marcos
>   Facultad de Medicina. Universidad de Morón
>   Machado 914. B1708JPD. Buenos Aires. Argentina
>   e-mail alternativo hernanjavier <@t> yahoo.com
>   web: http://hjaldanamarcos.bravepages.com
>   http://histologia.bigthicketdirectory.net/main.html
>
>     ----- Original Message -----
>     From: Jill Cox
>     To: histonet <@t> lists.utsouthwestern.edu
>     Sent: Wednesday, December 03, 2003 1:58 PM
>     Subject: [Histonet] Stain for H.Pylori
>
>
>     Hello everyone,
>      Has anyone done a Toluidine blue/Alcian yellow stain
> for H. Pylori? If so how do the Doctors like it?   Or,
> what other stains are you doing for that. I am trying to
> get away from the Genta!! Thanks in advance
>
>
>
>     Jill Cox HT (ASCP)
>     Histology Supervisor
>     Seattle Histology Lab
>
>
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