[Histonet] fixing bone marrow for IHC

[Patsy Ruegg]

Why would you do antigen retrieval on tissue not formalin fixed?
Patsy Ruegg

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of MARY T HODGES
Sent: Thursday, November 27, 2003 9:30 AM
To: Rcartun <@t> harthosp.org; histonet <@t> lists.utsouthwestern.edu;
scoop <@t> mail.nih.gov
Subject: Re: [Histonet] fixing bone marrow for IHC

Good Morning,
   I have used Methanol and acetone for smears and had great results. 15
mins... let air dry ...15 mins and let air dry. They do well even through
antigen retrival. good luck
Tere Hodges
St Mary's Hospital
Tucson,Az.


>From: "Richard Cartun" <Rcartun <@t> harthosp.org>
>To: <histonet <@t> lists.utsouthwestern.edu>,<scoop <@t> mail.nih.gov>
>Subject: Re: [Histonet] fixing bone marrow for IHC
>Date: Tue, 25 Nov 2003 16:56:06 -0500
>
>We use formalin.  I hate to see anyone use B5 because of environmental
>concerns with mercury.
>
>Richard Cartun
>Director, Immunopathology
>Hartford Hospital
>
> >>> Sharon Cooperman <scoop <@t> mail.nih.gov> 11/25/03 04:16PM >>>
>Does anyone have an opinion on the best way to fix bone marrow smears
>for immunohistochemistry?  So far I've heard methanol, acetone, B-5
>and formalin.  The antibodies I plan to use all work on formalin
>fixed paraffin embedded tissue.  I'd appreciate any advice or
>opinions.
>
>Thanks,
>Sharon
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
online games and music with a high-speed Internet connection!  Prices start
at less than $1 a day average.  https://broadband.msn.com (Prices may vary
by service area.)


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet