[Histonet] HIER using B & D rice steamer

[amosbrooks <@t> earthlink.net]

Linda,
     The steamer is absolutly the best method of retrieval around. The heating is even and can be directly monitored in real time. If you drill a hole in the top of the steamer you can put a thermometer directly into the solution. The steamer is not subject to the temperature spikes you see using a microwave. The price is usually right. The steamers can usually be found for under $50.00. I highly reccommend it. If you have any questions just ask:-).
Amos Brooks

BTW: Be careful with the hole in the top. I have heard of someone that had a hard plastic top and it broke on him. I believe the Black & Decker has a soft plastic top (that's what I use) so it should be no problem.


Message: 10
Date: Mon, 1 Dec 2003 16:12:34 -0600
From: "Sebree Linda A." <la.sebree <@t> hosp.wisc.edu>
To: "Histonet (E-mail)" <histonet <@t> pathology.swmed.edu>
Cc: "Slukvin Igor I" <ISLUKVIN <@t> WISC.EDU>
Subject: [Histonet] HIER using B & D rice steamer

One of our pathologists is looking for an inexpensive way to antigen =
retrieve tissues for staining with CD3, CD68 and CD56.  I suggested a =
Black and Decker rice steamer although I personally have no experience =
with one.  Is anyone out there willing to share their retrieval =
protocol?  I (and he) would greatly appreciate it.

Thank you,

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174