[Histonet] HIER using B & D rice steamer
TMcNemar <@t> lmhealth.org
Tue Dec 2 05:11:01 CST 2003
We use the B&D steamer for all retrievals. We preheat the reagent for 5
minutes then add the slides for 20 minutes. Remove them and allow them to
cool for another 20 minutes. They then sit in buffer for another 5 minutes
before putting them on the Dako Autostainer.
Tom Mc Nemar HT(ASCP)
Licking Memorial Hospital
From: Sebree Linda A. [mailto:la.sebree <@t> hosp.wisc.edu]
Sent: Monday, December 01, 2003 5:13 PM
To: Histonet (E-mail)
Cc: Slukvin Igor I
Subject: [Histonet] HIER using B & D rice steamer
One of our pathologists is looking for an inexpensive way to antigen
retrieve tissues for staining with CD3, CD68 and CD56. I suggested a Black
and Decker rice steamer although I personally have no experience with one.
Is anyone out there willing to share their retrieval protocol? I (and he)
would greatly appreciate it.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
600 Highland Ave.
Madison, WI 53792
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