[Histonet] H&E to PAS staining

Johnson, Teri TJJ <@t> Stowers-Institute.org
Mon Dec 1 13:45:41 CST 2003

I've done this in the past and found that the Periodic Acid removes
Hematoxylin.  The eosin (most of it) was removed in the hydrating
alcohols and water after removing the mounting medium.  If you want
absolute quality control, you might consider H&E staining your
control slide and then use the destain "protocol" John Kiernan
provided, but I doubt most people will go to this length.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, Missouri  64110
tjj <@t> stowers-institute.org

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