[Histonet] Formalin blues

Nick Kirk nick.kirk3 <@t> btopenworld.com
Mon Aug 25 13:19:11 CDT 2003

Novocastra do one that works on formalin fixed paraffin processed tissue.

Go to http://www.novocastra.co.uk/data/cam/Ig-super/cd311a10.pdf for further

Nick Kirk
Head BMS
Hinchingbrooke Hospital

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Christian
Sent: 25 August 2003 18:31
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Formalin blues

I'm studying neo-vascularization.
It seems that the only antibodies to CD31 (murine and Human) that I can find
commercially (BD Bio and Chemicon) do not work at all for formalin fixed,
paraffin imbedded tissue sections.

Does anyone out there know of any anti CD31 Ab's that DO work in formalin
fixed tissues?

Also, does anyone know of any Ab that I could use as a positive control (for
both mouse and human tissues) that works well in formalin-fixed samples?

Lastly, does anyone have a better fixation method to formalin (compatible
with paraffin imbedding) which may allow me to use the above mentioned
commercial Ab's?

Thanks for your help!

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