[Histonet] cyclin-D1

Mcleod hmcleod <@t> chempath.uct.ac.za
Mon Aug 25 08:34:54 CDT 2003

Dear All

What is the preferred AR method for Cyclin-D1's?  We have the Dako (cat
no M7155) clone DCS-6 antibody and while it works well as a marker for
the cell cycle we have not had any success with Mantle Cell Lymphomas. 
We have tried the "standard" AR methods i.e. HIER at pH6 and pH.8 in a
pressure cooker at full power for 2 mins. as well as protease on our
Ventana Nexes system. Any help and suggestions very welcome.  

Thanks a stack in advance

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