[Histonet] IHC- using IgM vs. IgG

[Majid Ghoddusi]

Hello dear histonetters
 
Just a very basic question for IHC experts:
 
if your primary antibody is a mouse IgM, by using a secondary anti-mouse IgG
are you running any risk  of getting artefactual signals?
 
Thanks very much in advance for any help and advice including pointing to a
reference that might discuss this in detail.
 
 
 
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Majid Ghoddusi 
Children's Medical Research Institute
Locked Bag 23, Wentworthville NSW 2145
Tel: (02) 9687-2800
Fax:(02) 9687-2120
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