[Histonet] warthin starry stain
Lee & Peggy Wenk
lpwenk <@t> mail.netquest.com
Sat Aug 23 08:44:13 CDT 2003
It's probably because the doctor/nurse is letting the biopsy dry out before they place it in formalin. Even setting a minute on a pad will do this. We've seen this also with kidney biopsies.
No way to correct it after the fact. No way to make the W/S stain work. The tissue HAS to be placed in the fixative IMMEDIATELY.
You might try the Giemsa or Diff-Quik stain. Sometimes they will still stain the H. pylorii after this drying out.
Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073
----- Original Message -----
To: histonet <@t> pathology.swmed.edu
Sent: Friday, August 22, 2003 6:28 PM
Subject: [Histonet] warthin starry stain
I am having some trouble with our Warthin-Starry stain. The peripheri of the gastric biopsy is not getting stained. The rest of the specimen is well stained. I am not sure if I need to lengthen the impregnation time or the developer time. I have never had this happen before. Any suggestions would be greatly appreciated.
Celia, HT Sioux Valley Hospital
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