[Histonet] cryostat decontamination

Charles W. Scouten, Ph.D. cwscouten <@t> myneurolab.com
Thu Aug 21 10:21:46 CDT 2003

With the Vibratome 7500 Cryostat, decontamination involves pressing a button and doing something else for 3.5 hours, after which the instrument is thoroughly decontaminated, again cold, and ready to cut tissue.  Before the next decontamination cycle, empty the waste bin down the sink, and refill a bottle of water and a bottle of Perascope decontamination fluid.  That's all.  For more information on how this is accomplished, see the following link:


Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
cwscouten <@t> myneurolab.com 

-----Original Message-----
From: Bill Sinai [mailto:bills <@t> icpmr.wsahs.nsw.gov.au] 
Sent: Wednesday, August 20, 2003 4:57 PM
To: Laurie Colbert; histonet
Subject: Re: [Histonet] cryostat decontamination


We have a protocol for decontaminating all our Cryostats once per week.  We
have 2 Shandon 660E models which have the heating block which you fill with
40% formadlehyde then turn on the decontamination cycle.  This is performed
overnight Friday.  After this process the shavings are removed using tissue
damped with 70% ethanol.
The others have a formaldehyde "bomb" prepared and placed into the chamber,
then left sealed to defrost for 24 hours.  These are then cleaned using 70%

The same procedures are carried out if we have cut anything suspected of
being an infective agent, immediately after the case is finished.

We have the luxury of two cryostats available in our main laboratory.

All the best
Bill Sinai
Laboratory Manager
Tissue Pathology
P.O. Box 533
Wentworthville NSW 2145
pH 02 9845 7774
----- Original Message -----
From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, August 21, 2003 5:03 AM
Subject: [Histonet] cryostat decontamination

How do others clean the cryostat after a known or suspected TB case?  Also,
do you decontaminate once a week with a tuberculocidal antiseptic?

Laurie Colbert
Huntington Memorial Hospital

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