[Histonet] sudan black B
Jose Luis Palazon Fernandez
jluis.palazon <@t> icman.csic.es
Thu Aug 21 02:59:07 CDT 2003
Dear List members.
I need your advice
I stained some slides with sudan black B. I used 4 slides per specimen and I did (delipidized, sudan black B, Bromide-sudan black, and bromide-acetone-sudan black). I have noticed that the slides were clearly black after staining, the bromide treated the most black, but after 1-2 weeks, they are turning green in color. Is the color of sudan black B stained slides not permanent? must the slides be read inmediately? or do this change in color alter the results if I read some recently stained slides and other stained some weeks ago?. Is it possible (adequate) to put the coverlips out and re-stain the slides? I would appreciate some advice from you.
Thanks in advance
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