[Histonet] Marine histology

[Kathleen Roberts]

Never mind-it turned out that this is a baby seahorse, quite small (I
originally thought it was one of the big ones, split into several
cassettes)-he won't take long at all to decalcify, and we figure that we
can process it the same way as we do the rest of our samples.

Thanks anyway-
Kathleen

Kathleen Roberts wrote:

> Good morning Histonetters-
>
> I just got a seahorse from one of our university vets-he wants it
> processed & stained with H& E.  It was fixed in Formalde-fresh buffered
> formalin for nine days, and it needs to be decalcified.  Would anybody
> happen to know how long it would take a seahorse to decalcify?  I've
> done fish before, but not seahorses.  Any advice on processing would be
> welcome as well.
>
> (Yes, I know about the book Dr. Callis wrote on animal tissue
> processing; it hasn't gotten here yet!  :o)
>
> Thanks for all your help-
> Kathleen Roberts
> Principal Lab Technician
> Molecular Pathology Core Facility
> Dept of Pharmacology & Toxicology
> Ernest Mario School of Pharmacy
> Rutgers University
> 41 B Gordon Rd
> Piscataway, NJ 08854
>
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