[Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green

[Carl Hobbs]

Hi Histonet
Pros and cons re these two methods?
I usually carry out PSR for collagen
( I use the Great  John Kiernan's modification )
Collagen is red, all other tissues are yellow
A new Researcher insists on S.red-Fast Green (SrFg) for quantification
HOWEVER, I note that this also contains Picric acid
How does the Picric acid interact with Fast Green?
Is there a yellow in the SrFg method??
Be grateful for incites before I make this

Be most grateful for advice

Carl


Carl Hobbs FIBMS
Histology and Imaging Manager
Sensory, Pain and Regeneration Centre,
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6810