From melissa at alliedsearchpartners.com Wed Apr 1 07:58:09 2026 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 1 Apr 2026 12:58:09 +0000 Subject: [Histonet] Discussion on managing coverage gaps for MOHS Surgeries Message-ID: Hi everyone, I wanted to open a discussion around something I'm seeing more frequently across MOHS with my dermatology clients and MOHS Surgeon clients. Traditional paraffin histology has a bit more leeway than MOHS histology when it comes to being suddenly short staffed. How is everyone in the dermatopathology lab currently handling last-minute coverage gaps in MOHS or understaffed for MOHS Techs? Cross-training internal staff? Adjusting schedules on the fly? Building in backup coverage? Limiting case load when short-staffed? Would love to hear what's working (and what's not) across different practices for any dermatopathology lab out there. I think this is an area where sharing approaches could really help everyone navigate the day-to-day challenges more effectively. Looking forward to the discussion. Best, Melissa-Founder/CEO Allied Search Partners From jmacdonald at mtsac.edu Wed Apr 1 14:06:32 2026 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 1 Apr 2026 19:06:32 +0000 Subject: [Histonet] =?windows-1252?q?California_Society_for_Histotechnolog?= =?windows-1252?q?y_Annual_Meeting_=96_May_14=9617=2C_2026?= Message-ID: Fixation is key? for tissue and connections. Join the California Society for Histotechnology for an engaging conference focused on advancing histotechnology through education and collaboration. Date: May 14?17, 2026 Location: Costa Mesa Highlights include: * Hands-on workshops * Practical, real-world techniques * Networking with fellow histology professionals Don?t let this opportunity float away?register today: https://www.californiahistology.org/events Jennifer MacDonald From relia1 at earthlink.net Tue Apr 7 09:49:07 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 7 Apr 2026 10:49:07 -0400 Subject: [Histonet] Meet Your Next Histotech in Under 14 Days Message-ID: <008501dcc69d$b1ca69f0$155f3dd0$@earthlink.net> Hi there Histonetters! Hope you?re doing well today. If your lab is starting to think about spring or summer staffing, I?ve got a strong group of histotechs who can move quickly many of them can land in the right role in under 14 days. I keep things simple and steady with: ? Direct, targeted outreach (no job board noise) ? Pre?screened candidates who fit your shift, skill needs, and culture ? Clear communication so you always know what?s happening ? Current Candidate Snapshot ?and in most cases, ready to move for April or early May. ? Histology Managers & Supervisors FL, TN, CA, NC, TX, AZ, AL Open to relocation for the right opportunity ? Histology Technicians & Technologists ASCP?certified ? CLIA?qualified to gross FL, TX, AL, AZ, VA, PA, OK, KY, IN Flexible on location depending on schedule, culture, and growth ? What makes this group different: ? Proven, reliable performers ? Strong technical depth ? Represented exclusively through RELIA ?? And when they decide to move, things tend to move fast, often under 14 days from first conversation to offer. If you?re looking ahead to April hires, or just want a clearer sense of what?s out there, this is a great time to talk. I?m happy to share profiles, timelines, or simply walk through what might fit your lab. Warmly, Pam The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From Sandy at hradvantageconsultants.com Tue Apr 7 11:45:43 2026 From: Sandy at hradvantageconsultants.com (Sandy Benghiat) Date: Tue, 7 Apr 2026 16:45:43 +0000 Subject: [Histonet] Seeking a QA/Compliance Director in Miami, FL. Message-ID: This is a Leadership Role. Primary role is ensuring that day-to-day clinical trial operations are conducted in compliance with study protocols. * $140k-150k Salary Range. * Resumes Pl. Forward to: Sandy at HRadvantageconsultants.com Thank you! From relia1 at earthlink.net Wed Apr 8 11:59:36 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 8 Apr 2026 12:59:36 -0400 Subject: [Histonet] Should I Stay or Should I Go? A Career Litmus Test for Histotechs Message-ID: <000001dcc779$165b88a0$431299e0$@earthlink.net> Hi there Histonetters, If you?ve been feeling the shift in the histology world lately, you?re not imagining it. Travelers are seeing contracts change, and permanent techs are feeling the weight of uncertainty. None of this means you need to make a sudden move ? but it is a good time to check in with yourself. I just published a new blog that walks through a simple litmus test to help you get clarity on whether staying where you are still serves you ? or whether it might be time to explore new options. Read the blog here: ? https://www.linkedin.com/pulse/should-i-stay-go-career-litmus-test-histology-pam-barker-12k7f/ I?m working with labs nationwide on HT/HTL roles, leadership positions, and specialty opportunities that aren?t always posted publicly. If you?re starting to wonder what else might be out there ? or whether now is the right time to explore ? reach out anytime. I?m always happy to share what I?m seeing in the market and talk through options that fit your experience and goals. If you?re at a crossroads or simply want clarity, I?m here. Sometimes the next step isn?t a job change. Sometimes it?s just a conversation. Warmly, Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From gu.lang at gmx.at Sat Apr 11 11:18:32 2026 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 11 Apr 2026 18:18:32 +0200 Subject: [Histonet] question regarding pH in retrieval solutions Message-ID: <000301dcc9ce$d82595a0$8870c0e0$@gmx.at> Hi all! Although I am rather long in the business, I came across a question, that I wanted to share with you. With IHC-HIER one uses high-pH tris-buffers at about pH 8-9 and low-pH citrate-buffers at pH 6. Tris-buffers are sensible to changes in temperature ? the higher the temperature, the lower the pH. A tris-buffer of pH 8,5 at roomtemperatur should have about pH 6,5 at 98?C (if my KI-friend calculates correctly). The citrate-buffer is not affected by temperature. So as a result the actual pH in the retrieval-solution would be rather the same. That makes me thinking... Where is the difference? Why do high-pH buffer work mostly better? Am I totally wrong with my assumptions? I would be happy about any input. kind regards Gudrun Lang Gudrun Lang Biomedizinische Analytikerin Austria From carl.hobbs at kcl.ac.uk Sat Apr 11 13:06:40 2026 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 11 Apr 2026 18:06:40 +0000 Subject: [Histonet] re question regarding pH in retrieval solutions (Gudrun Lang) Message-ID: I hope I set my email to plain text...will find out! To paraphrase The Excellent Leonard Cohen's song: Nobody knows ? Chuckle Citric acid HIER soln is not a buffer if only Citric acid is used NaOH is used to adjust pH to 6 To make the Citrate a buffer, one would have to use Citric acid and sodium citrate I use just Citric acid adjusted to pH6 using NaOH TRIS is, as it is buffered using HCL to pH9 Do any buffers behave as buffers, at such high temperatures? After Shi, Catoretti was The Man to nail it....imho Imho, TRIS v Citra HIER rarely work equally well One or the other ( or neither ) works on Pwax sections I have no XP of TRIS v Citra working equally with any antibody I have not achieved ever, a better result incorporating EDTA into my TRIS pH9 So, for my lab: no "EDTA- sequestering of calcium bound to proteins" is effective My basic understanding is that high heat induces a dipole moment in many proteins( twisting without fragmentation) thus epitopes are "revealed", for a time Need the high heat because the proteins are Formalin-fixed ( if I leave my HIER sections overnight before probing with primary antibody I have a sig loss- of antigenicity) If I perform HIER on Formalin - fixed cryosections, I can obtain a similar immunoreactivity if I subject sections to 90C HIER....only after an extended HIER time However, never as high a dilution factor for primary ab I posted images on the excellent IHC site, several yrs ago....sadly no longer in existence Well, that is my "rather long in the business" opinion, Gudrun Discuss? Best wishes Carl From gu.lang at gmx.at Sun Apr 12 03:36:02 2026 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 12 Apr 2026 10:36:02 +0200 Subject: [Histonet] re question regarding pH in retrieval solutions Message-ID: <000301dcca57$66420340$32c609c0$@gmx.at> Hi Carl, I am always slightly overhelmed by your answers with my humble school-English ?. We work with the Roche instruments and detectionsystems. They describe the high-pH AR-solution as "buffer on Tris-basis" and the low-pH AR-solution as "citrate-buffer". So I have to believe, that those reagens are actually buffers. And according to literature pH plays a role in the retrieval-process. Maybe I need some lessons in chemistry about pH, dissoziation of H+ in buffers and water. Apparently even pure water changes pH with high temperatures and gets slighty acidic. Boiling in hot water also does the AR-job to a certain degree. Boiling brakes short-range-bonds in proteins and leads to denaturation (and/or koagulation). Denaturation should also play an important part in AR. Boiling causes also the reversal of the binding of methylol-adducts from formaldehyd - and therefore frees the epitopes. With the calcium-binding-theory I have troubles, because I don't see where the calcium comes from. There are some antigens (like E-Cadherin) that need calcium for stabilization of the tertiary-structure. But are there so many of them? But why do high-pH and low-pH buffers work different, if the actual working pH is the same? One idea is, that it is not the boiling-part but the cooling-part. How the proteins fold again during the cool-down would presumably depend on the pH-milieu. best wishes Gudrun -----Urspr?ngliche Nachricht----- Von: Carl Hobbs via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Samstag, 11. April 2026 20:07 An: histonet Betreff: [Histonet] re question regarding pH in retrieval solutions (Gudrun Lang) I hope I set my email to plain text...will find out! To paraphrase The Excellent Leonard Cohen's song: Nobody knows ? Chuckle Citric acid HIER soln is not a buffer if only Citric acid is used NaOH is used to adjust pH to 6 To make the Citrate a buffer, one would have to use Citric acid and sodium citrate I use just Citric acid adjusted to pH6 using NaOH TRIS is, as it is buffered using HCL to pH9 Do any buffers behave as buffers, at such high temperatures? After Shi, Catoretti was The Man to nail it....imho Imho, TRIS v Citra HIER rarely work equally well One or the other ( or neither ) works on Pwax sections I have no XP of TRIS v Citra working equally with any antibody I have not achieved ever, a better result incorporating EDTA into my TRIS pH9 So, for my lab: no "EDTA- sequestering of calcium bound to proteins" is effective My basic understanding is that high heat induces a dipole moment in many proteins( twisting without fragmentation) thus epitopes are "revealed", for a time Need the high heat because the proteins are Formalin-fixed ( if I leave my HIER sections overnight before probing with primary antibody I have a sig loss- of antigenicity) If I perform HIER on Formalin - fixed cryosections, I can obtain a similar immunoreactivity if I subject sections to 90C HIER....only after an extended HIER time However, never as high a dilution factor for primary ab I posted images on the excellent IHC site, several yrs ago....sadly no longer in existence Well, that is my "rather long in the business" opinion, Gudrun Discuss? Best wishes Carl _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Sun Apr 12 11:26:42 2026 From: amosbrooks at gmail.com (Amos Brooks) Date: Sun, 12 Apr 2026 12:26:42 -0400 Subject: [Histonet] question regarding pH in retrieval solutions In-Reply-To: References: Message-ID: Hi, I always adjust the pH of the solutions at room temperature. What happens to the pH as the temperature changes should at least be consistent. So as long as there is a consistent starting point and temperature change to result in good antigen retrieval, I presume it is a reproducible process whatever the final pH ends up being. If the end result is consistent then I have done everything possible to maintain it. This is more or less an acceptance of a variable that I can't really control for. In the words of Karl Paul Reinhold Niebuhr: "The victorious man in the day of crisis is the man who has the serenity to accept what he cannot help and the courage to change what must be altered. All the Best, Amos Brooks ---------------------------------------------------------------------- > Message: 1 > Date: Sat, 11 Apr 2026 18:18:32 +0200 > From: "Gudrun Lang" > To: > Subject: [Histonet] question regarding pH in retrieval solutions > Message-ID: <000301dcc9ce$d82595a0$8870c0e0$@gmx.at> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all! > > Although I am rather long in the business, I came across a question, that I > wanted to share with you. > > With IHC-HIER one uses high-pH tris-buffers at about pH 8-9 and low-pH > citrate-buffers at pH 6. > > Tris-buffers are sensible to changes in temperature ? the higher the > temperature, the lower the pH. > > > > A tris-buffer of pH 8,5 at roomtemperatur should have about pH 6,5 at 98?C > (if my KI-friend calculates correctly). > > The citrate-buffer is not affected by temperature. > > > > So as a result the actual pH in the retrieval-solution would be rather the > same. That makes me thinking... > > Where is the difference? Why do high-pH buffer work mostly better? Am I > totally wrong with my assumptions? > > > > I would be happy about any input. > > kind regards > > Gudrun Lang > > From carl.hobbs at kcl.ac.uk Sun Apr 12 12:20:50 2026 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sun, 12 Apr 2026 17:20:50 +0000 Subject: [Histonet] Histonet Digest, Vol 269, Issue 6 In-Reply-To: References: Message-ID: I agree, Amos Empiricism rules, in this context HIER mechanism is still ???....tho we have figured it out, to great effect! Maybe, to paraphrase: "The victorious person in the day of crisis is the person who has the serenity to accept what they cannot help and the courage to change what must be altered." However, how do we know what cannot be helped? Paradigm shifts are always required? Luverly to read Histonet exchanges again! Best wishes Carl ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: 12 April 2026 18:00 To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 269, Issue 6 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Ccarl.hobbs%40kcl.ac.uk%7C3eb3ad6ca33a45d172d408de98b57e0f%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C639116102454698583%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=BwAlpZn%2BZ%2BGSPSTcvC2Z5sLo5b%2BWMya7yqkgXxI787k%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. re question regarding pH in retrieval solutions (Gudrun Lang) (Carl Hobbs) 2. Re: re question regarding pH in retrieval solutions (Gudrun Lang) 3. question regarding pH in retrieval solutions (Amos Brooks) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Apr 2026 18:06:40 +0000 From: Carl Hobbs To: histonet Subject: [Histonet] re question regarding pH in retrieval solutions (Gudrun Lang) Message-ID: Content-Type: text/plain; charset="iso-8859-1" I hope I set my email to plain text...will find out! To paraphrase The Excellent Leonard Cohen's song: Nobody knows ? Chuckle Citric acid HIER soln is not a buffer if only Citric acid is used NaOH is used to adjust pH to 6 To make the Citrate a buffer, one would have to use Citric acid and sodium citrate I use just Citric acid adjusted to pH6 using NaOH TRIS is, as it is buffered using HCL to pH9 Do any buffers behave as buffers, at such high temperatures? After Shi, Catoretti was The Man to nail it....imho Imho, TRIS v Citra HIER rarely work equally well One or the other ( or neither ) works on Pwax sections I have no XP of TRIS v Citra working equally with any antibody I have not achieved ever, a better result incorporating EDTA into my TRIS pH9 So, for my lab: no "EDTA- sequestering of calcium bound to proteins" is effective My basic understanding is that high heat induces a dipole moment in many proteins( twisting without fragmentation) thus epitopes are "revealed", for a time Need the high heat because the proteins are Formalin-fixed ( if I leave my HIER sections overnight before probing with primary antibody I have a sig loss- of antigenicity) If I perform HIER on Formalin - fixed cryosections, I can obtain a similar immunoreactivity if I subject sections to 90C HIER....only after an extended HIER time However, never as high a dilution factor for primary ab I posted images on the excellent IHC site, several yrs ago....sadly no longer in existence Well, that is my "rather long in the business" opinion, Gudrun Discuss? Best wishes Carl ------------------------------ Message: 2 Date: Sun, 12 Apr 2026 10:36:02 +0200 From: "Gudrun Lang" To: "'Carl Hobbs'" Cc: Subject: Re: [Histonet] re question regarding pH in retrieval solutions Message-ID: <000301dcca57$66420340$32c609c0$@gmx.at> Content-Type: text/plain; charset="utf-8" Hi Carl, I am always slightly overhelmed by your answers with my humble school-English ?. We work with the Roche instruments and detectionsystems. They describe the high-pH AR-solution as "buffer on Tris-basis" and the low-pH AR-solution as "citrate-buffer". So I have to believe, that those reagens are actually buffers. And according to literature pH plays a role in the retrieval-process. Maybe I need some lessons in chemistry about pH, dissoziation of H+ in buffers and water. Apparently even pure water changes pH with high temperatures and gets slighty acidic. Boiling in hot water also does the AR-job to a certain degree. Boiling brakes short-range-bonds in proteins and leads to denaturation (and/or koagulation). Denaturation should also play an important part in AR. Boiling causes also the reversal of the binding of methylol-adducts from formaldehyd - and therefore frees the epitopes. With the calcium-binding-theory I have troubles, because I don't see where the calcium comes from. There are some antigens (like E-Cadherin) that need calcium for stabilization of the tertiary-structure. But are there so many of them? But why do high-pH and low-pH buffers work different, if the actual working pH is the same? One idea is, that it is not the boiling-part but the cooling-part. How the proteins fold again during the cool-down would presumably depend on the pH-milieu. best wishes Gudrun -----Urspr?ngliche Nachricht----- Von: Carl Hobbs via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Samstag, 11. April 2026 20:07 An: histonet Betreff: [Histonet] re question regarding pH in retrieval solutions (Gudrun Lang) I hope I set my email to plain text...will find out! To paraphrase The Excellent Leonard Cohen's song: Nobody knows ? Chuckle Citric acid HIER soln is not a buffer if only Citric acid is used NaOH is used to adjust pH to 6 To make the Citrate a buffer, one would have to use Citric acid and sodium citrate I use just Citric acid adjusted to pH6 using NaOH TRIS is, as it is buffered using HCL to pH9 Do any buffers behave as buffers, at such high temperatures? After Shi, Catoretti was The Man to nail it....imho Imho, TRIS v Citra HIER rarely work equally well One or the other ( or neither ) works on Pwax sections I have no XP of TRIS v Citra working equally with any antibody I have not achieved ever, a better result incorporating EDTA into my TRIS pH9 So, for my lab: no "EDTA- sequestering of calcium bound to proteins" is effective My basic understanding is that high heat induces a dipole moment in many proteins( twisting without fragmentation) thus epitopes are "revealed", for a time Need the high heat because the proteins are Formalin-fixed ( if I leave my HIER sections overnight before probing with primary antibody I have a sig loss- of antigenicity) If I perform HIER on Formalin - fixed cryosections, I can obtain a similar immunoreactivity if I subject sections to 90C HIER....only after an extended HIER time However, never as high a dilution factor for primary ab I posted images on the excellent IHC site, several yrs ago....sadly no longer in existence Well, that is my "rather long in the business" opinion, Gudrun Discuss? Best wishes Carl _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Ccarl.hobbs%40kcl.ac.uk%7C3eb3ad6ca33a45d172d408de98b57e0f%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C639116102454722308%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=HSO8PDOkFkwDORtagv65tkIshW738MXdAKXNKhPVt2Y%3D&reserved=0 ------------------------------ Message: 3 Date: Sun, 12 Apr 2026 12:26:42 -0400 From: Amos Brooks To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] question regarding pH in retrieval solutions Message-ID: Content-Type: text/plain; charset="UTF-8" Hi, I always adjust the pH of the solutions at room temperature. What happens to the pH as the temperature changes should at least be consistent. So as long as there is a consistent starting point and temperature change to result in good antigen retrieval, I presume it is a reproducible process whatever the final pH ends up being. If the end result is consistent then I have done everything possible to maintain it. This is more or less an acceptance of a variable that I can't really control for. In the words of Karl Paul Reinhold Niebuhr: "The victorious man in the day of crisis is the man who has the serenity to accept what he cannot help and the courage to change what must be altered. All the Best, Amos Brooks ---------------------------------------------------------------------- > Message: 1 > Date: Sat, 11 Apr 2026 18:18:32 +0200 > From: "Gudrun Lang" > To: > Subject: [Histonet] question regarding pH in retrieval solutions > Message-ID: <000301dcc9ce$d82595a0$8870c0e0$@gmx.at> > Content-Type: text/plain; charset="iso-8859-1" > > Hi all! > > Although I am rather long in the business, I came across a question, that I > wanted to share with you. > > With IHC-HIER one uses high-pH tris-buffers at about pH 8-9 and low-pH > citrate-buffers at pH 6. > > Tris-buffers are sensible to changes in temperature ? the higher the > temperature, the lower the pH. > > > > A tris-buffer of pH 8,5 at roomtemperatur should have about pH 6,5 at 98?C > (if my KI-friend calculates correctly). > > The citrate-buffer is not affected by temperature. > > > > So as a result the actual pH in the retrieval-solution would be rather the > same. That makes me thinking... > > Where is the difference? Why do high-pH buffer work mostly better? Am I > totally wrong with my assumptions? > > > > I would be happy about any input. > > kind regards > > Gudrun Lang > > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://eur03.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Ccarl.hobbs%40kcl.ac.uk%7C3eb3ad6ca33a45d172d408de98b57e0f%7C8370cf1416f34c16b83c724071654356%7C0%7C0%7C639116102454740469%7CUnknown%7CTWFpbGZsb3d8eyJFbXB0eU1hcGkiOnRydWUsIlYiOiIwLjAuMDAwMCIsIlAiOiJXaW4zMiIsIkFOIjoiTWFpbCIsIldUIjoyfQ%3D%3D%7C0%7C%7C%7C&sdata=WYFITHkefX0wvfK7QQibXxUxbm1pcGJo5VCiqQ6Zl1o%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 269, Issue 6 **************************************** From jkiernan at uwo.ca Sun Apr 12 15:01:19 2026 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 12 Apr 2026 20:01:19 +0000 Subject: [Histonet] re question regarding pH in retrieval solutions In-Reply-To: <000301dcca57$66420340$32c609c0$@gmx.at> References: <000301dcca57$66420340$32c609c0$@gmx.at> Message-ID: In connection with the pH of boiling-hot solutions, I asked Google the questions: "How do you measure the pH of a boiling hot solution? Is there a standard buffer for hot solutions?" The AI answer was far too long (4 or 5 screenfuls) for a Histonet reply, beginning with: Measuring the pH of a boiling hot solution (near 100C) is difficult because temperature drastically changes both the solution's chemical properties and the sensor's physical response. You must use specialized high-temperature sensors and account for the fact that "neutral" pH at 100C is approximately 6.14, not 7.00. 1. How to Measure Boiling Solutions. High-Temperature Electrodes: Standard pH probes are usually rated only up to 60-80C. For boiling solutions, you must use a glass body electrode with high-temperature electrolyte and a specialized reference system designed to resist "poisoning" or drying out at high heat. .... If you ask the same question, you should get the same long answer, which has some references at the end. I didn't follow these up. John Kiernan, London, Ontario, Canada. = = = ________________________________ From: Gudrun Lang via Histonet Sent: Sunday, April 12, 2026 3:36 AM To: 'Carl Hobbs' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] re question regarding pH in retrieval solutions Hi Carl, I am always slightly overhelmed by your answers with my humble school-English ?. We work with the Roche instruments and detectionsystems. They describe the high-pH AR-solution as "buffer on Tris-basis" and the low-pH AR-solution as "citrate-buffer". So I have to believe, that those reagens are actually buffers. And according to literature pH plays a role in the retrieval-process. Maybe I need some lessons in chemistry about pH, dissoziation of H+ in buffers and water. Apparently even pure water changes pH with high temperatures and gets slighty acidic. Boiling in hot water also does the AR-job to a certain degree. Boiling brakes short-range-bonds in proteins and leads to denaturation (and/or koagulation). Denaturation should also play an important part in AR. Boiling causes also the reversal of the binding of methylol-adducts from formaldehyd - and therefore frees the epitopes. With the calcium-binding-theory I have troubles, because I don't see where the calcium comes from. There are some antigens (like E-Cadherin) that need calcium for stabilization of the tertiary-structure. But are there so many of them? But why do high-pH and low-pH buffers work different, if the actual working pH is the same? One idea is, that it is not the boiling-part but the cooling-part. How the proteins fold again during the cool-down would presumably depend on the pH-milieu. best wishes Gudrun -----Urspr?ngliche Nachricht----- Von: Carl Hobbs via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Samstag, 11. April 2026 20:07 An: histonet Betreff: [Histonet] re question regarding pH in retrieval solutions (Gudrun Lang) I hope I set my email to plain text...will find out! To paraphrase The Excellent Leonard Cohen's song: Nobody knows ? Chuckle Citric acid HIER soln is not a buffer if only Citric acid is used NaOH is used to adjust pH to 6 To make the Citrate a buffer, one would have to use Citric acid and sodium citrate I use just Citric acid adjusted to pH6 using NaOH TRIS is, as it is buffered using HCL to pH9 Do any buffers behave as buffers, at such high temperatures? After Shi, Catoretti was The Man to nail it....imho Imho, TRIS v Citra HIER rarely work equally well One or the other ( or neither ) works on Pwax sections I have no XP of TRIS v Citra working equally with any antibody I have not achieved ever, a better result incorporating EDTA into my TRIS pH9 So, for my lab: no "EDTA- sequestering of calcium bound to proteins" is effective My basic understanding is that high heat induces a dipole moment in many proteins( twisting without fragmentation) thus epitopes are "revealed", for a time Need the high heat because the proteins are Formalin-fixed ( if I leave my HIER sections overnight before probing with primary antibody I have a sig loss- of antigenicity) If I perform HIER on Formalin - fixed cryosections, I can obtain a similar immunoreactivity if I subject sections to 90C HIER....only after an extended HIER time However, never as high a dilution factor for primary ab I posted images on the excellent IHC site, several yrs ago....sadly no longer in existence Well, that is my "rather long in the business" opinion, Gudrun Discuss? Best wishes Carl _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa at alliedsearchpartners.com Mon Apr 13 08:01:55 2026 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Mon, 13 Apr 2026 13:01:55 +0000 Subject: [Histonet] Anatomic Pathology Jobs from Allied Search Partners Message-ID: Happy Monday- Just wanted to put some of our Anatomic Pathology jobs out here. Please contact me if you are interested in learning more. 1-Central California: Seeking Travel/Locums or Permanent ASCP Certified/Accredited Pathology Assistant Schooled -Pathologist Assistant 2-Just south of Poughkeepsie, NY: Seeking a Pathologist Assistant (can be non-certified PA/experience in grossing only needed) for Per Diem work 3-Westchester County, NY: Seeking Pathologist Assistant Permanent/Full Time (can be non-certified PA/experienced in grossing only needed) 4-Concord, CA area: Seeking MOHS Tech for Permanent/Full Time (1 year experience required). 5-Westchester County, NY: Advancement opportunity for a Histotech or Cytotech with 6+ years experience- team lead, chief, or supervisory experience to step into an Anatomic Pathology Management role 6-Westchester County, NY: Full Time Permanent Histotech Have a great week! Melissa Owens Allied Search Partners From Nilufa.Sultana at uts.edu.au Mon Apr 13 08:59:10 2026 From: Nilufa.Sultana at uts.edu.au (Nilufa Sultana) Date: Mon, 13 Apr 2026 13:59:10 +0000 Subject: [Histonet] Please unsubscribe me Message-ID: Please unsubscribe me from this list. Thank you Kind regards Nilufa Sultana Get Outlook for iOS UTS CRICOS Provider Code: 00099F DISCLAIMER: This email message and any accompanying attachments may contain confidential information. If you are not the intended recipient, do not read, use, disseminate, distribute or copy this message or attachments. If you have received this message in error, please notify the sender immediately and delete this message. Any views expressed in this message are those of the individual sender, except where the sender expressly, and with authority, states them to be the views of the University of Technology Sydney. Before opening any attachments, please check them for viruses and defects. Think. Green. Do. Please consider the environment before printing this email. From annigyg at gmail.com Mon Apr 13 12:25:39 2026 From: annigyg at gmail.com (Anne van Binsbergen) Date: Mon, 13 Apr 2026 19:25:39 +0200 Subject: [Histonet] MOHS quick IHC Message-ID: <78E25405-037F-455B-BE24-7823ECCC0CB3@gmail.com> Hello you lot - it?s been a minute Getting a bit tired of only seeing US job opportunities on this platform. Starting to look like a Linked-in for Histo Hopefully some of the old guard are around to help/advise on some real Histology issues. So I have retired from my ?annieinarabia? days and am now ?annieinafrica? again I was retired for 3 years because of Covid and I have un-retired and hooked up with a MOHS-mad dermatologist. MOHS rocks and now I?m sniffing around for CK 5(AE1/AE3) fast (10mins @ RT) IHC for frozen sections. Anyone out there have any dealings with NovoDiax? Missed you all. A lot. Would love to hear from you. Annie in Africa From relia1 at earthlink.net Wed Apr 15 10:56:01 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 15 Apr 2026 11:56:01 -0400 Subject: [Histonet] This Wednesday: A Better Way to Work With Recruiters Message-ID: <001001dcccf0$5e485bd0$1ad91370$@earthlink.net> Hi there Histonetters, Just a quick little note for mid-week. Things are a bit quieter right now, and you may see some postings out there, but not all of them are places I?d ever feel good sending you. I?d rather wait than put something in front of you that isn?t truly worth your time. If this year follows the same rhythm as 2025, the really strong openings tend to show up around May and June. So just stay tuned. There?s usually a moment where everything moves at once, and I want you in a good spot when it does. While things are calm, I put together a short guide on working with recruiters in a way that actually supports your career. It?s simple, clear, and meant to make the whole process feel a little easier. You can read it here: ? https://www.linkedin.com/pulse/better-way-work-recruiters-pam-barker-p8m5c If the link isn't live you can copy and paste it into your browser or Visit my page on LinkedIn: https://www.linkedin.com/in/reliasolutions/ If you ever want to talk through what you?re hoping for next, I?m here. Warmly, Pam "The secret of getting ahead is getting started." - Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407 353 5070 866 60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407 353 5070 866 60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From melissa at alliedsearchpartners.com Thu Apr 16 07:14:58 2026 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 16 Apr 2026 12:14:58 +0000 Subject: [Histonet] Histology Information & Finding it on Social Media Message-ID: Hello- Did you know that 11.6 billion people are on social media 2+ hours a day? Social media and online communities have become a huge part of how professionals learn, connect, troubleshoot and have camaraderie in our global community. I just started a new Facebook group called H&E Cafe-more of a fun, real-talk space for histology lab life. The goal is to create a social media space where histology professionals can unite. Please feel free to log in to Facebook, search up H&E Caf? and join us in building an information platform for histology on social media. Thank you, Melissa Allied Search Partners From rsrichmond at gmail.com Thu Apr 16 13:27:04 2026 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 16 Apr 2026 14:27:04 -0400 Subject: [Histonet] Histonet Digest, Vol 269, Issue 10 In-Reply-To: References: Message-ID: I looked up H&E Cafe in Facebook and found an entirely different Facebook site. On Thu, Apr 16, 2026 at 1:03?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Histology Information & Finding it on Social Media (Melissa Owens) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 16 Apr 2026 12:14:58 +0000 > From: Melissa Owens > To: "'histonet at lists.utsouthwestern.edu'" > > Subject: [Histonet] Histology Information & Finding it on Social Media > Message-ID: > < > MN2PR11MB42887864882CC0AD27D184EFC3232 at MN2PR11MB4288.namprd11.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > Hello- > > Did you know that 11.6 billion people are on social media 2+ hours a day? > Social media and online communities have become a huge part of how > professionals learn, connect, troubleshoot and have camaraderie in our > global community. > > I just started a new Facebook group called H&E Cafe-more of a fun, > real-talk space for histology lab life. The goal is to create a social > media space where histology professionals can unite. > > Please feel free to log in to Facebook, search up H&E Caf? and join us in > building an information platform for histology on social media. > > Thank you, > > Melissa > Allied Search Partners > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 269, Issue 10 > ***************************************** > From nmargaryan88 at gmail.com Thu Apr 16 13:37:09 2026 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Thu, 16 Apr 2026 13:37:09 -0500 Subject: [Histonet] Histonet Digest, Vol 269, Issue 10 In-Reply-To: References: Message-ID: Agree! There is nothing about histology, but a lot of food and coffee On Thu, Apr 16, 2026 at 1:33?PM Bob Richmond via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I looked up H&E Cafe in Facebook and found an entirely different Facebook > site. > > On Thu, Apr 16, 2026 at 1:03?PM > > wrote: > > > Send Histonet mailing list submissions to > > histonet at lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request at lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner at lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. Histology Information & Finding it on Social Media (Melissa Owens) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Thu, 16 Apr 2026 12:14:58 +0000 > > From: Melissa Owens > > To: "'histonet at lists.utsouthwestern.edu'" > > > > Subject: [Histonet] Histology Information & Finding it on Social Media > > Message-ID: > > < > > > MN2PR11MB42887864882CC0AD27D184EFC3232 at MN2PR11MB4288.namprd11.prod.outlook.com > > > > > > > Content-Type: text/plain; charset="iso-8859-1" > > > > Hello- > > > > Did you know that 11.6 billion people are on social media 2+ hours a day? > > Social media and online communities have become a huge part of how > > professionals learn, connect, troubleshoot and have camaraderie in our > > global community. > > > > I just started a new Facebook group called H&E Cafe-more of a fun, > > real-talk space for histology lab life. The goal is to create a social > > media space where histology professionals can unite. > > > > Please feel free to log in to Facebook, search up H&E Caf? and join us in > > building an information platform for histology on social media. > > > > Thank you, > > > > Melissa > > Allied Search Partners > > > > > > ------------------------------ > > > > Subject: Digest Footer > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > > > End of Histonet Digest, Vol 269, Issue 10 > > ***************************************** > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Mon Apr 20 10:12:14 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 20 Apr 2026 11:12:14 -0400 Subject: [Histonet] Happy Medical Laboratory Professionals Week - A moment for people who make healthcare possible Message-ID: <000901dcd0d8$134fc320$39ef4960$@earthlink.net> Hello Histopeeps and Histonetters!, Happy Medical Laboratory Professionals Week! This week, we celebrate the lab professionals whose quiet, precise work makes healthcare possible. Histotechs, lab techs, supervisors, and managers bring clarity to every diagnosis. I shared a short reflection on that, along with a simple guide to making the most of Lab Week. Read it here: https://www.linkedin.com/pulse/quiet-professionals-powerful-impact-honoring-medical-week-pam-barker-ih6re Thank you for the skill, dedication, and professionalism you bring to the bench every day. If you ever need support ? whether it?s exploring new opportunities or strengthening your team, I?m here. Warm regards, Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From relia1 at earthlink.net Wed Apr 22 12:02:27 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 22 Apr 2026 13:02:27 -0400 Subject: [Histonet] =?utf-8?q?The_Work_Behind_the_Answers_=E2=80=93_Opport?= =?utf-8?q?unities_for_Your_Next_Step?= Message-ID: <000001dcd279$ced47c60$6c7d7520$@earthlink.net> Hi Histopeeps and Histonetters! Lab Week is a reminder of how much precision, focus, and care goes into every result, and professionals like you are the reason patients get the answers they need. Your Work Matters More Than Most People Ever See, And It Doesn?t Go Unnoticed. If you?re considering a change or simply curious about what?s out there, I have several strong opportunities available ? including one I?m highlighting this week. RELIA Spotlight Opportunity ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Run Your Own Dermpath/Mohs Lab ? Seattle, Wa * Full?Time, Permanent Role. * Excellent Physicians, * Strong Compensation * The Chance To Truly Own The Workflow. ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? Additional Leadership & HT/HTL Roles Openings across: ? Florida ? California ? Tennessee ? Wisconsin ? Washington More roles are coming online over the next 30?45 days, so if you have a location in mind, now is a great time to let me know. If you?d like details, a confidential conversation, or help mapping out your next step, I?m here. Warm regards, Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From relia1 at earthlink.net Fri Apr 24 09:36:29 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 24 Apr 2026 10:36:29 -0400 Subject: [Histonet] =?utf-8?q?=F0=9F=8E=89_Happy_Friday=2E_Happy_Lab_Week?= =?utf-8?q?=2E_You_earned_this_celebration=2E?= Message-ID: <000401dcd3f7$bfab7520$3f025f60$@earthlink.net> Hi There Histonetters! I hope this wraps up an amazing Lab Week 2026 for you! All week long, behind every slide, every stain, every ?can you take a look at this,? there?s been a whole crew of lab pros showing up with skill, grit, humor, and the kind of excellence that keeps patient care moving. Today is your victory lap. Here?s to the people who fire up the microtome like it?s second nature? Pull off the clutch recuts? Solve problems before anyone else even knows they?re there? Keep the vibe steady when the day goes sideways? And still find a way to laugh through the chaos. Histology doesn?t run on luck ? it runs on you. The steady hands. The sharp eyes. The quiet leaders who make the whole system work. This week wasn?t just about celebrating the profession ? it was about celebrating the people who give it heart, soul, and momentum. Here?s to the teamwork, the grit, the wins, the wild moments, and the humans who make patient care possible in ways most will never see. You didn?t just get through Lab Week ? you crushed it. Thanks-Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From melissa at alliedsearchpartners.com Fri Apr 24 12:34:14 2026 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Fri, 24 Apr 2026 17:34:14 +0000 Subject: [Histonet] Temp and Permanent South Florida Job Openings Message-ID: Hello- I have a Temp need for a client in Boca area. We have a day shift or evening shift need for a histotech temporarily. Please reach out if you have availability. I also have a Miami, FL area permanent role for Histotech with Electron Microscopy experience. Please reach out directly for details. Thank you, Melissa, Allied Search Partners From carl.hobbs at kcl.ac.uk Fri Apr 24 13:56:47 2026 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Fri, 24 Apr 2026 18:56:47 +0000 Subject: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green Message-ID: Hi Histonet Pros and cons re these two methods? I usually carry out PSR for collagen ( I use the Great John Kiernan's modification ) Collagen is red, all other tissues are yellow A new Researcher insists on S.red-Fast Green (SrFg) for quantification HOWEVER, I note that this also contains Picric acid How does the Picric acid interact with Fast Green? Is there a yellow in the SrFg method?? Be grateful for incites before I make this Be most grateful for advice Carl Carl Hobbs FIBMS Histology and Imaging Manager Sensory, Pain and Regeneration Centre, Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From paula at excaliburpathology.com Fri Apr 24 14:40:03 2026 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Fri, 24 Apr 2026 19:40:03 +0000 (UTC) Subject: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green In-Reply-To: References: Message-ID: <150140669.2820831.1777059603312@mail.yahoo.com> Hi, everything that stains yellow with SrPa will be green with SrFg. With structures staining red and green, a person that is color blind would have difficulty determining the difference. This is reason aniline blue is used with trichrome as an alternative to green. However, the birefringence of the collagen under polarized light should be the same.? Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, April 24, 2026 at 02:06:36 PM CDT, Carl Hobbs via Histonet wrote: Hi Histonet Pros and cons re these two methods? I usually carry out PSR for collagen ( I use the Great? John Kiernan's modification ) Collagen is red, all other tissues are yellow A new Researcher insists on S.red-Fast Green (SrFg) for quantification HOWEVER, I note that this also contains Picric acid How does the Picric acid interact with Fast Green? Is there a yellow in the SrFg method?? Be grateful for incites before I make this Be most grateful for advice Carl Carl Hobbs FIBMS Histology and Imaging Manager Sensory, Pain and Regeneration Centre, Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Sat Apr 25 00:42:07 2026 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 25 Apr 2026 05:42:07 +0000 Subject: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green In-Reply-To: <150140669.2820831.1777059603312@mail.yahoo.com> References: <150140669.2820831.1777059603312@mail.yahoo.com> Message-ID: I never tried the PSR+fast green FCF method. It's worth remembering that birefringence under polarized light is seen only with bundles of collagen fibres. Individual thin collagen fibres (reticular fibres) and basement membranes (as in renal glomeruli) stain red but are not birefringent. PSR is much easier than traditional (silver) methods. Thanks, Paula Pierce for bringing up red-green colour blindness. It's something I'd never thought of in the context of Mallory-type staining. John Kiernan = = = ________________________________ From: Paula Keene Pierce via Histonet Sent: Friday, April 24, 2026 2:40 PM To: histonet ; Carl Hobbs Subject: Re: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green Hi, everything that stains yellow with SrPa will be green with SrFg. With structures staining red and green, a person that is color blind would have difficulty determining the difference. This is reason aniline blue is used with trichrome as an alternative to green. However, the birefringence of the collagen under polarized light should be the same. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Friday, April 24, 2026 at 02:06:36 PM CDT, Carl Hobbs via Histonet wrote: Hi Histonet Pros and cons re these two methods? I usually carry out PSR for collagen ( I use the Great John Kiernan's modification ) Collagen is red, all other tissues are yellow A new Researcher insists on S.red-Fast Green (SrFg) for quantification HOWEVER, I note that this also contains Picric acid How does the Picric acid interact with Fast Green? Is there a yellow in the SrFg method?? Be grateful for incites before I make this Be most grateful for advice Carl Carl Hobbs FIBMS Histology and Imaging Manager Sensory, Pain and Regeneration Centre, Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Sat Apr 25 02:22:07 2026 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 25 Apr 2026 09:22:07 +0200 Subject: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green In-Reply-To: References: Message-ID: <000601dcd484$3a246eb0$ae6d4c10$@gmx.at> Hi Carl, I try a guess. In trichrome-stains the molecular-weight and the dye-structure are important. In PSR it is rather a bichrome-stain with picroacid as small dye and siriusred as big dye, with affinitiy to collagen fibers. Usually the small dye binds to cytoplasmic proteins, the big one to collagen. In the Picro-Sirius red-Fast Green stain are three dyes involved, and Picroacid is the smallest of them, then comes Fast Green and then Sirius red. The structure of Fast Green is related to Acid Fuchsin, that is also used as cytoplasma-dye in trichromes (and is smaller than Fast Green). I think the Picroacid would be the first to stain cytoplasma but competes with Fast Green and is replaced by the green dye during incubation. - But it is still important for holding the acidic pH in the staining solution. I never have done the Sirius Red - Fast Green stain, but in theory it would be like that. ; ) I hope anyone, who knows better, will correct any mistake. kind regards Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Carl Hobbs via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 24. April 2026 20:57 An: histonet Betreff: [Histonet] PicroSirius red ( PSR) versus Sirius red - Fast Green Hi Histonet Pros and cons re these two methods? I usually carry out PSR for collagen ( I use the Great John Kiernan's modification ) Collagen is red, all other tissues are yellow A new Researcher insists on S.red-Fast Green (SrFg) for quantification HOWEVER, I note that this also contains Picric acid How does the Picric acid interact with Fast Green? Is there a yellow in the SrFg method?? Be grateful for incites before I make this Be most grateful for advice Carl Carl Hobbs FIBMS Histology and Imaging Manager Sensory, Pain and Regeneration Centre, Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Apr 27 11:49:36 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 27 Apr 2026 12:49:36 -0400 Subject: [Histonet] A Role That Might Interest You. Message-ID: <008701dcd665$d671b790$835526b0$@earthlink.net> Hi there Histonetters, Happy Monday. I?m reaching out because I have a leadership opportunity that might be a strong next step for someone with your background. I?m working with a derm/Mohs group in Seattle we are looking for someone to: Run Your Own Derm/Mohs Lab. * Full Autonomy, Great Physicians, * A Setup Where Your Experience Really Matters. * It?s A Full-Time, Permanent Role * Excellent Compensation * A Very Collaborative Environment. Histopeeps, I?m curious how something like this feels to you. Seattle has been a popular move for many techs, but everyone?s situation is different. I?m just opening the door in case this is something you?d consider or want to hear more about. If you?d like details, I?m happy to share more or talk through what you?re looking for next. If someone in your circle might be a great fit, feel free to pass this along ? Warmly, Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From relia1 at earthlink.net Wed Apr 29 12:35:45 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 29 Apr 2026 13:35:45 -0400 Subject: [Histonet] Thinking About A Job Change? Read this First! Message-ID: <003101dcd7fe$9e3794e0$daa6bea0$@earthlink.net> Hi there Histonetters, Hope you're doing well. If you?re considering a change later this year, this is a great time to get organized. Many candidates are using this stretch to: ? Update resumes and ASCP documentation ? Clarify what they want next ? Check in with references ? Make sure everything is ready for the next opportunity I also just published a new blog post that walks through how to prepare for a job change in histology. It?s a quick, practical read that covers what to consider, how to stay ready, and whether travel or permanent roles make the most sense for you right now. Read it Here: https://www.linkedin.com/feed/update/urn:li:activity:7455291484016390144/ If you?re looking now or in the very near future, I also have several full-time permanent openings that may be a fit. These roles offer excellent compensation, strong benefits, and relocation assistance. Current opportunities include: * Mohs Techs * IHC Specialists, * Molecular and Flow, * Supervisor/Manager Lead Tech roles * Histotechnician and Histotechnologist roles. My active openings are in: Oklahoma, Tennessee, Florida, California Washington Wisconsin, with new locations coming in daily. If something looks close but not quite right, whether it?s the role or the location, let me know so I can BOLO ? be on the lookout for YOU. If you?d like help reviewing your resume or talking through what you?re aiming for, I?m here anytime. Thanks-Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results. From SThompson4 at sonichealthcareusa.com Wed Apr 29 17:32:19 2026 From: SThompson4 at sonichealthcareusa.com (Stephanie Thompson) Date: Wed, 29 Apr 2026 22:32:19 +0000 Subject: [Histonet] Supervisor Opportunity in Beachwood Ohio - Sonic Healthcare USA Message-ID: Great opportunity for someone with a histology background and supervisory experience. Relocation is available. Location: Beachwood, Ohio Days: Monday - Friday Full Time/Benefit Eligible In this role, you will: * Lead laboratory operations with a focus on identifying areas of opportunity and implementing action plans. * Support the Regional Manager with organizational goals and objectives, including developing and inspiring your team of employees. * Interact with a variety of clients, patients, employees, and business units, with a commitment to customer-focused service. * Review business indicators, optimize processes, and maximize profitability. * Champion safety, compliance, and quality control. All you need is: * Strong knowledge of laboratory operations. * Pathology knowledge. * Histology background a plus Education: * Bachelors? degree in a field of science * Previous experience in a clinical laboratory or other service organization. We?ll give you: * Appreciation for your work * A feeling of satisfaction that you?ve helped people * Opportunity to grow within the organization * Free lab services for you and your eligible dependents * Work-life balance, including Paid Time Off and Paid Holidays * Competitive benefits including medical, dental, and vision insurance * Help saving for retirement, with a 401(k) that includes a generous company match Send your resume to: sthompson4 at sonichealthcareusa.com From relia1 at earthlink.net Thu Apr 30 11:34:00 2026 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 30 Apr 2026 12:34:00 -0400 Subject: [Histonet] IHC Opportunity in Tulsa Message-ID: <000201dcd8bf$28536760$78fa3620$@earthlink.net> Hi there, Histopeeps and Histonetters! I hope you are having a fantastic day! I?m working on a RELIA Exclusive IHC Technologist role with a strong, growing lab in Tulsa, OK. I?m reaching out because I?ve been hearing from techs in your area who are open to a move here for the right fit. The role includes full IHC workflow, antibody optimization, QC, troubleshooting, and supporting newer staff. The lab is also expanding, with future needs in Flow, Molecular, and HT/HTL. If You?d Like Details On The Team, Compensation, Or Relocation Support, I?m Happy To Share More. And If You Know Someone Who May Be Interested, Feel Free To Pass My Info Along. relia1 at earthlink.net | 407?353?5070 Have a Great Day! Thanks-Pam "The secret of getting ahead is getting started." ? Mark Twain Pam Barker Histology Recruitment | RELIA Solutions Winter Park, FL 407?353?5070 866?60RELIA relia1 at earthlink.net Trusted expertise for labs that need results.