From edmartin26 at gmail.com Thu May 1 11:54:07 2025 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 1 May 2025 12:54:07 -0400 Subject: [Histonet] Histonet Digest, Vol 257, Issue 11 In-Reply-To: References: Message-ID: Hi John, A few common scenarios of why recoverslipping would be needed may give you the better response you're searching for. An example may be that the lab is diluting their coverslip medium too much and slides look great for a few days, or another scenario, where they forget to prime the tubing in their instruments or the viscosity is too thick, causing air to get dispensed rather than coverslipping medium. Instead of a re-coverslipping percentage, what information are you looking for? Best wishes. Eddie Martin National Institutes of Health On Tue, Apr 29, 2025 at 1:00?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Removing H&E coverslippped slide (John O?Brien) > > > > ---------- Forwarded message ---------- > From: "John O?Brien" > To: histonet at lists.utsouthwestern.edu > Cc: > Bcc: > Date: Mon, 28 Apr 2025 11:50:40 -0700 > Subject: [Histonet] Removing H&E coverslippped slide > > Histonetters > Can you experts in staining and Coverslipping offer the number of times > or % of slides recover slipped to a differ stain, any opinion is > appreciated > John > IMEB Inc > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From edmartin26 at gmail.com Thu May 1 11:57:18 2025 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 1 May 2025 12:57:18 -0400 Subject: [Histonet] Histonet Digest, Vol 257, Issue 10 In-Reply-To: References: Message-ID: Carl, That's really cool that Histonet was storing histology images. Sad to hear this after the fact. I'm wondering what kind of histology images? Were format were you saving them? Thanks, Eddie Martin On Sun, Apr 20, 2025 at 1:00?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Histology images (Carl Hobbs) > > > > ---------- Forwarded message ---------- > From: Carl Hobbs > To: histonet > Cc: > Bcc: > Date: Sat, 19 Apr 2025 17:53:33 +0000 > Subject: [Histonet] Histology images > Hi > I used to upload my Histology images to Histonet Images archive > I have been informed that this no longer exists: hence my images are > "LOST" > I'd be grateful if somebody would suggest where best to upload my images, > to share? > > It's good to share-ily > Carl > > > Carl Hobbs FIBMS > Histology and Imaging Manager > Wolfson SPaRC > Guys Campus, London Bridge > Kings College London > London > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john at imebinc.com Thu May 1 12:29:21 2025 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Thu, 1 May 2025 10:29:21 -0700 Subject: [Histonet] Histonet Digest, Vol 258, Issue 1 In-Reply-To: References: Message-ID: <6014A8CA-3161-48C0-ABAF-6BF31749693B@imebinc.com> Hi Mr. Martin Thank you for responce, I am a supplier of pathology supplies and instruments and we keep getting asked can our coverslipped slides be removed and re stained and recoverslipped, we have developed mounting media that is xylene free and can coverslip straight out of alcohol or coverslip dry , It?s a special plastic mix that dries in 30 seconds with UV light, Problem is it?s difficult to remove coverslip cleanly thus trying determine % of slides to be recoverslipped would help with understanding how it is big or small issue Any help is appreciated Regards John IMEB > On May 1, 2025, at 10:13?AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Re: Histonet Digest, Vol 257, Issue 11 (Eddie Martin) > 2. Re: Histonet Digest, Vol 257, Issue 10 (Eddie Martin) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 1 May 2025 12:54:07 -0400 > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Digest, Vol 257, Issue 11 > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hi John, > > A few common scenarios of why recoverslipping would be needed may give you > the better response you're searching for. An example may be that the lab > is diluting their coverslip medium too much and slides look great for a few > days, or another scenario, where they forget to prime the tubing in their > instruments or the viscosity is too thick, causing air to get > dispensed rather than coverslipping medium. > > Instead of a re-coverslipping percentage, what information are you looking > for? > > Best wishes. > > Eddie Martin > National Institutes of Health > > >> On Tue, Apr 29, 2025 at 1:00?PM >> wrote: >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> Today's Topics: >> >> 1. Removing H&E coverslippped slide (John O?Brien) >> >> >> >> ---------- Forwarded message ---------- >> From: "John O?Brien" >> To: histonet at lists.utsouthwestern.edu >> Cc: >> Bcc: >> Date: Mon, 28 Apr 2025 11:50:40 -0700 >> Subject: [Histonet] Removing H&E coverslippped slide >> >> Histonetters >> Can you experts in staining and Coverslipping offer the number of times >> or % of slides recover slipped to a differ stain, any opinion is >> appreciated >> John >> IMEB Inc >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 2 > Date: Thu, 1 May 2025 12:57:18 -0400 > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Digest, Vol 257, Issue 10 > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Carl, > > That's really cool that Histonet was storing histology images. Sad to > hear this after the fact. I'm wondering what kind of histology images? > Were format were you saving them? > > Thanks, > Eddie Martin > > > > On Sun, Apr 20, 2025 at 1:00?PM > wrote: > >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> Today's Topics: >> >> 1. Histology images (Carl Hobbs) >> >> >> >> ---------- Forwarded message ---------- >> From: Carl Hobbs >> To: histonet >> Cc: >> Bcc: >> Date: Sat, 19 Apr 2025 17:53:33 +0000 >> Subject: [Histonet] Histology images >> Hi >> I used to upload my Histology images to Histonet Images archive >> I have been informed that this no longer exists: hence my images are >> "LOST" >> I'd be grateful if somebody would suggest where best to upload my images, >> to share? >> >> It's good to share-ily >> Carl >> >> >> Carl Hobbs FIBMS >> Histology and Imaging Manager >> Wolfson SPaRC >> Guys Campus, London Bridge >> Kings College London >> London >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 258, Issue 1 > **************************************** From amosbrooks at gmail.com Mon May 5 04:07:09 2025 From: amosbrooks at gmail.com (Amos Brooks) Date: Mon, 5 May 2025 05:07:09 -0400 Subject: [Histonet] Histonet Digest, Vol 258, Issue 2 In-Reply-To: References: Message-ID: Hi, I do not use this mounting media.we use a traditional xylene based resin. We recoverslip very often. Sometimes it is a poorly coverslipped slide or one that ended up with two coverslips on the slide. That happens occasionally. More frequently however is that there is a request to have another stain on a specific slide. So, the coverslip would be removed and either the prior stain is destained or simply stained over the prior one. In the interest of not wasting tissue from the block or when a block is not available due to being either exhausted, sent out to another institution or simply misplaced this is needed on occasion. I would find an inability to remove a coverslip an extreme red flag when it comes to a choice of coverslipping methods. Even if you rarely need this, the one time you do makes the inability to do so a huge problem. For my lab, I would never paint myself into a corner by using a product that eliminates possible downstream applications. Amos Brooks ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 1 May 2025 10:29:21 -0700 > From: John O?Brien > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Digest, Vol 258, Issue 1 > Message-ID: <6014A8CA-3161-48C0-ABAF-6BF31749693B at imebinc.com> > Content-Type: text/plain; charset=utf-8 > > Hi Mr. Martin > Thank you for responce, > I am a supplier of pathology supplies and instruments and we keep getting > asked can our coverslipped slides be removed and re stained and > recoverslipped, we have developed mounting media that is xylene free and > can coverslip straight out of alcohol or coverslip dry , > It?s a special plastic mix that dries in 30 seconds with UV light, > Problem is it?s difficult to remove coverslip cleanly thus trying > determine % of slides to be recoverslipped would help with understanding > how it is big or small issue > Any help is appreciated > Regards > John > IMEB > From Jennifer.Olson at centracare.com Tue May 6 15:29:44 2025 From: Jennifer.Olson at centracare.com (Olson, Jennifer (Carris Lab)) Date: Tue, 6 May 2025 20:29:44 +0000 Subject: [Histonet] staining over IHC stained slide Message-ID: I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. From CObregon at mhs.net Tue May 6 15:37:22 2025 From: CObregon at mhs.net (Obregon, Cecilia) Date: Tue, 6 May 2025 20:37:22 +0000 Subject: [Histonet] staining over IHC stained slide In-Reply-To: References: Message-ID: I do not recommend this. Staining over an already stained IHC can compromise both antigen integrity and staining quality due to previous antigen retrieval. We have however done this on previously stained H&E, just keep in mind you might have to run a modified protocol with 'no-depar' and run a separate slide with a positive control. Thank you Cecilia M. Obregon -----Original Message----- From: Olson, Jennifer (Carris Lab) via Histonet Sent: Tuesday, May 6, 2025 4:30 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] staining over IHC stained slide ====================================================================================== THIS EMAIL ORIGINATED FROM OUTSIDE OF MHS. PLEASE EXERCISE CAUTION WITH ATTACHMENTS, LINKS, OR REQUESTED ACTIONS. ====================================================================================== I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!LqvVzw!QjS3SG1UuL4IAqfDPuQuxeJasW20L88lnc_M-XPw4jTt217GANzqRc6CSOzG87Rqj5KIif0HM_HNhhEjzJ56_IxgDoke$ From rcartun at sbcglobal.net Tue May 6 19:00:33 2025 From: rcartun at sbcglobal.net (richard cartun) Date: Wed, 7 May 2025 00:00:33 +0000 (UTC) Subject: [Histonet] staining over IHC stained slide In-Reply-To: References: Message-ID: <239178017.2284300.1746576033499@mail.yahoo.com> We did this a lot when I was working at Hartford Hospital.? This was especially useful when the lesion or cells of interest in the paraffin block were consumed during microtomy.? The pathologist would select previously tested slides that were negative, or had minimal immunoreactivity, and then reorder the IHC test(s) in the computer, specifying which slide to use for which antibody.? New labels were prepared, and we would then carefully remove the coverslip and then rehydrate to tap water.? There is no need to remove the eosin (which will come out in the alcohol) or hematoxylin (which you want anyway).? No need to quench endogenous peroxidase again, but you will need to perform or repeat the appropriate heat-induced epitope retrieval (HIER) when indicated.? If there is immunoreactivity present in the original slide, you could re-test using a different chromogen to facilitate interpretation.? Obviously, if there is no immunoreactivity in the lesion or cells of interest in the repeat slide, and there is no internal control in the specimen to validate the negative result, the negative test result cannot be reported. Richard W. Cartun, MS, PhDMorphologic Proteomics, LLCAvon, CT? USA On Tuesday, May 6, 2025 at 04:30:18 PM EDT, Olson, Jennifer (Carris Lab) via Histonet wrote: I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at pathologyarts.com Wed May 7 10:18:52 2025 From: c.tague at pathologyarts.com (Curt Tague) Date: Wed, 7 May 2025 15:18:52 +0000 Subject: [Histonet] staining over IHC stained slide In-Reply-To: <239178017.2284300.1746576033499@mail.yahoo.com> References: <239178017.2284300.1746576033499@mail.yahoo.com> Message-ID: Very insightful response, thank you! Get Outlook for iOS ________________________________ From: richard cartun via Histonet Sent: Tuesday, May 6, 2025 5:00:33 PM To: histonet at lists.utsouthwestern.edu ; Olson, Jennifer (Carris Lab) Subject: Re: [Histonet] staining over IHC stained slide We did this a lot when I was working at Hartford Hospital. This was especially useful when the lesion or cells of interest in the paraffin block were consumed during microtomy. The pathologist would select previously tested slides that were negative, or had minimal immunoreactivity, and then reorder the IHC test(s) in the computer, specifying which slide to use for which antibody. New labels were prepared, and we would then carefully remove the coverslip and then rehydrate to tap water. There is no need to remove the eosin (which will come out in the alcohol) or hematoxylin (which you want anyway). No need to quench endogenous peroxidase again, but you will need to perform or repeat the appropriate heat-induced epitope retrieval (HIER) when indicated. If there is immunoreactivity present in the original slide, you could re-test using a different chromogen to facilitate interpretation. Obviously, if there is no immunoreactivity in the lesion or cells of interest in the repeat slide, and there is no internal control in the specimen to validate the negative result, the negative test result cannot be reported. Richard W. Cartun, MS, PhDMorphologic Proteomics, LLCAvon, CT USA On Tuesday, May 6, 2025 at 04:30:18 PM EDT, Olson, Jennifer (Carris Lab) via Histonet wrote: I have a pathologist who is inquiring about staining over and already IHC stained slide. He would like to stain over using a different antibody that has been stained. He doesn't have a specific case or antibody, he is just wondering if there are any histology labs out there that do this? Any suggestions? Thank you - Confidentiality Notice: This e-mail and any attachment may contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this transmission in error, please notify the sender immediately, reply to this transmission, or contact the CentraCare Information Systems Network Security staff by calling the IS Help Desk for assistance at 320-251-2700, ext. 54540, and delete these documents. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=HKQvKuj2FgpDyQ2Txjw7zPeScXjrUkgrQ3HZD5448fo--6pvAuuFyUos2q5yhIjb&s=Uxu3ZdXA9WFE4yaLbn123OsV8H2Z7Y485KbyvfAqm-0&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIGaQ&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=HKQvKuj2FgpDyQ2Txjw7zPeScXjrUkgrQ3HZD5448fo--6pvAuuFyUos2q5yhIjb&s=Uxu3ZdXA9WFE4yaLbn123OsV8H2Z7Y485KbyvfAqm-0&e= From amosbrooks at gmail.com Wed May 7 13:47:39 2025 From: amosbrooks at gmail.com (Amos Brooks) Date: Wed, 7 May 2025 14:47:39 -0400 Subject: [Histonet] staining over IHC stained slide In-Reply-To: References: Message-ID: Hi, I do this frequently. It works great with some antibodies, but I have found some are less than cooperative about it. If you are concerned about it, and it would make sense if you were, it would be a good idea to take the control slide that was used and try it on that before doing it on patient test tissue. As I say thoi it usually works fine. Give it a try. Amos > > > -------------------------------------------------------------------- > > Message: 1 > Date: Tue, 6 May 2025 20:29:44 +0000 > From: "Olson, Jennifer (Carris Lab)" > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] staining over IHC stained slide > Message-ID: > < > BN8PR20MB264498E2F7984E71B2A8503FE789A at BN8PR20MB2644.namprd20.prod.outlook.com > > > > Content-Type: text/plain; charset="iso-8859-1" > > I have a pathologist who is inquiring about staining over and already IHC > stained slide. He would like to stain over using a different antibody that > has been stained. He doesn't have a specific case or antibody, he is just > wondering if there are any histology labs out there that do this? > > Any suggestions? > > Thank you - > > From jmacdonald at mtsac.edu Thu May 8 00:04:16 2025 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 8 May 2025 05:04:16 +0000 Subject: [Histonet] Use of Hg fixatives Message-ID: Is anyone still using any mercury fixatives, such as B5, Zenker, or Helly? Thanks, Jennifer Jennifer MacDonald Mt. San Antonio College From relia1 at earthlink.net Tue May 13 12:14:55 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 13 May 2025 13:14:55 -0400 Subject: [Histonet] Exclusive Opportunities in Beautiful Florida!! Message-ID: <007e01dbc42a$8d63a570$a82af050$@earthlink.net> Hi Histonetters! I hope this is the start to a fantastic week for you!! So Many Ways To Experience Our Beautiful State And I Have Exclusive Full Time Permanent Positions With Some Of The Best Companies Here In Florida And They Offer All Of The Things That Florida Has To Offer. Check It Out! The Florida License Is Easier Than Ever To Get And I Will Help You. My Clients Are Looking For Leaders, Experienced Techs And New GRADS! These Positions Are Full Time, Perm And Offer Relocation And Or A Sign On Bonus. I Have Opportunities In: South Florida SW Florida North Central Florida The Teams Are Well- Managed And Eager To Work With You. All You Have To Do Is Contact ME! Msg Me on Social Media OR E-Mail: mailto:relia1 at earthlink.net OR Cell/Text: 407-353-5070 Florida Not Your Bag? I Have Opportunities Nationwide Tell Me Where You Want To Go Or Stay And What You Want To Do. Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From relia1 at earthlink.net Thu May 15 12:42:44 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 15 May 2025 13:42:44 -0400 Subject: [Histonet] Histology Days and South Beach Nites!!! A RELIA Exclusive Opportunity in Southern Florida! Message-ID: <000001dbc5c0$c4105900$4c310b00$@earthlink.net> Hey Histopeeps! ? Histology Days and South Beach Nites! ? ? Intrigued? Sound like FUN? ? Check it out: I?m working on an EXCLUSIVE Opportunity in a Private Lab in South Florida! ? ?? Full-time, permanent M-F 8-5 ? Party on South Beach on the WEEKEND! ?? If you?re a Work Hard, Play Hard Histopeep, this could be the one for you! ? I can help you get your Florida license!! ? Want more info for you or your friends? ? ? Contact me: Cell/Text: 407-353-5070 ? Email: relia1 at earthlink.net ? ? Don?t miss out! Reach out today! ? Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?(407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From relia1 at earthlink.net Tue May 20 12:21:12 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 20 May 2025 13:21:12 -0400 Subject: [Histonet] =?utf-8?q?=F0=9F=8C=9E_Memorial_Day_Weekend_is_almost_?= =?utf-8?q?here=2C_histopeeps!_=F0=9F=8C=9F?= Message-ID: <003e01dbc9ab$9634e0c0$c29ea240$@earthlink.net> Hi Histonetters! As summer approaches, it?s the perfect time to think about fresh starts, exciting changes, and new opportunities. Every year, I hear from histology professionals ready to take the leap?whether it?s a new challenge, a different workplace, or a move to a dream location? Are you considering: ?? Exploring new career options? ?? Finding a role that truly fits your skills and lifestyle? ?? Moving closer to family or embracing a new adventure? I connect histotechs with Exclusive Opportunities that aren?t listed on big job boards. Let?s chat before the holiday weekend or anytime next week to get ahead of summer?s hiring surge! ? Reach out today: ? Toll-Free: 866-607-3542 ? Cell/Text: 407-353-5070 ? Email: relia1 at earthlink.net Let?s kick off summer the right way?with exciting new possibilities! Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From cforster at umn.edu Wed May 21 11:50:44 2025 From: cforster at umn.edu (Colleen Forster) Date: Wed, 21 May 2025 11:50:44 -0500 Subject: [Histonet] Pentachrome stain questions Message-ID: Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From relia1 at actsend.com Thu May 22 10:35:03 2025 From: relia1 at actsend.com (Pam Barker) Date: Thu, 22 May 2025 15:35:03 +0000 Subject: [Histonet] It's Memorial Day Weekend! Message-ID: Happy?Memorial?Day?Histonetters!?? Whatever your plans are I hope you make the most of them. Let?s honor our fallen heroes while cherishing the simple joys of summer. Looking forward to staying in touch. Pam ?Thank You! ?Pam M. Barker? 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Winter Springs, fl 32708 From gu.lang at gmx.at Thu May 22 12:59:04 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 22 May 2025 19:59:04 +0200 Subject: [Histonet] Pentachrome stain questions In-Reply-To: References: Message-ID: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick at northwestern.edu Thu May 22 14:22:20 2025 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Thu, 22 May 2025 19:22:20 +0000 Subject: [Histonet] Pentachrome stain questions In-Reply-To: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> References: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> Message-ID: I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ From colleen.forster at biosiminnovations.com Thu May 22 15:43:03 2025 From: colleen.forster at biosiminnovations.com (Colleen Forster) Date: Thu, 22 May 2025 20:43:03 +0000 Subject: [Histonet] Pentachrome stain questions In-Reply-To: References: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> Message-ID: Bernice, I am aware of this. That is the question. If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step? I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand. Colleen Forster ________________________________ From: Bernice Frederick via Histonet Sent: Thursday, May 22, 2025 2:22 PM To: Gudrun Lang ; 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions [Caution: This email originated from outside the organization.] I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIAL: This email (and any attachment) is confidential and may be protected by legal privilege, confidentiality agreements, and common or statutory law. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of this email or any attachment is prohibited. If you have received this email in error, please notify us at once by returning it to the sender and then deleting this copy from your system. Thank you. From jkiernan at uwo.ca Fri May 23 00:02:16 2025 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 23 May 2025 05:02:16 +0000 Subject: [Histonet] Pentachrome stain questions In-Reply-To: References: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> Message-ID: Why does anyone want to do the "pentachrome" method, which provided 4 (not 5) colours even in a perfectly stained section? See Lee Luna's "Manual of Histologic Staining Methods of the AFIP" (1968, p.95-97). Two of the dyes prescribed by HZ Movat (Arch. Path. 80:289-295) in the original 17-step method probably are no longer available. Russell's modification (21 steps!) is associated with a photo in the late Freida Carson's book. The intense red of a vessel wall makes the tiny surrounding (black) elastin fibres almost invisible. Freida's book provides various better and well illustrated elastin staining methods. Any correctly used alcian blue dye from a batch recently certified by the Biological Stain Commission will remain in place after minimal treatment with anything slightly alkaline, such as hard tap water. Check this out at https://biologicalstaincommission.org/current-issues/ Current Issues | The Biological Stain Commission Several issues have arisen since 2002, the year of publication of the 10th edition of Conn?s Biological Stains and of a detailed account of tests then used in the Commission?s assay laboratory (Penney et al. 2002), The following notes (alphabetically by names of dyes) refer to new tests, revised standards, and substances added to the list of stains for which certification is available. biologicalstaincommission.org John Kiernan = = = ________________________________ From: Colleen Forster via Histonet Sent: May 22, 2025 4:43 PM To: Gudrun Lang ; 'Colleen Forster' ; Bernice Frederick Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Bernice, I am aware of this. That is the question. If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step? I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand. Colleen Forster ________________________________ From: Bernice Frederick via Histonet Sent: Thursday, May 22, 2025 2:22 PM To: Gudrun Lang ; 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions [Caution: This email originated from outside the organization.] I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIAL: This email (and any attachment) is confidential and may be protected by legal privilege, confidentiality agreements, and common or statutory law. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of this email or any attachment is prohibited. If you have received this email in error, please notify us at once by returning it to the sender and then deleting this copy from your system. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Colleen Forster via Histonet Sent: May 22, 2025 4:43 PM To: Gudrun Lang ; 'Colleen Forster' ; Bernice Frederick Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Bernice, I am aware of this. That is the question. If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step? I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand. Colleen Forster ________________________________ From: Bernice Frederick via Histonet Sent: Thursday, May 22, 2025 2:22 PM To: Gudrun Lang ; 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions [Caution: This email originated from outside the organization.] I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIAL: This email (and any attachment) is confidential and may be protected by legal privilege, confidentiality agreements, and common or statutory law. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of this email or any attachment is prohibited. If you have received this email in error, please notify us at once by returning it to the sender and then deleting this copy from your system. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From colleen.forster at biosiminnovations.com Fri May 23 10:46:50 2025 From: colleen.forster at biosiminnovations.com (Colleen Forster) Date: Fri, 23 May 2025 15:46:50 +0000 Subject: [Histonet] Pentachrome stain questions In-Reply-To: References: <000601dbcb43$3c02deb0$b4089c10$@gmx.at> Message-ID: Thank you, John. You finally answered my initial question. In your opinion, does the pentachrome actually give you the old vs new collagen answer? I have a pathologist who orders the pentachrome like most doctors order an H/E stain. I will be doing a lot of these unless I can show a good alternative. As Always, Colleen Forster ________________________________ From: John Kiernan Sent: Friday, May 23, 2025 12:02 AM To: Gudrun Lang ; 'Colleen Forster' ; Bernice Frederick ; Colleen Forster Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Why does anyone want to do the "pentachrome" method, which provided 4 (not 5) colours even in a perfectly stained section? See Lee Luna's "Manual of Histologic Staining Methods of the AFIP" (1968, p.95-97). Two of the dyes prescribed by HZ Movat (Arch. Path. 80:289-295) in the original 17-step method probably are no longer available. Russell's modification (21 steps!) is associated with a photo in the late Freida Carson's book. The intense red of a vessel wall makes the tiny surrounding (black) elastin fibres almost invisible. Freida's book provides various better and well illustrated elastin staining methods. Any correctly used alcian blue dye from a batch recently certified by the Biological Stain Commission will remain in place after minimal treatment with anything slightly alkaline, such as hard tap water. Check this out at https://biologicalstaincommission.org/current-issues/ Current Issues | The Biological Stain Commission Several issues have arisen since 2002, the year of publication of the 10th edition of Conn?s Biological Stains and of a detailed account of tests then used in the Commission?s assay laboratory (Penney et al. 2002), The following notes (alphabetically by names of dyes) refer to new tests, revised standards, and substances added to the list of stains for which certification is available. biologicalstaincommission.org John Kiernan = = = ________________________________ From: Colleen Forster via Histonet Sent: May 22, 2025 4:43 PM To: Gudrun Lang ; 'Colleen Forster' ; Bernice Frederick Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Bernice, I am aware of this. That is the question. If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step? I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand. Colleen Forster ________________________________ From: Bernice Frederick via Histonet Sent: Thursday, May 22, 2025 2:22 PM To: Gudrun Lang ; 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions [Caution: This email originated from outside the organization.] I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIAL: This email (and any attachment) is confidential and may be protected by legal privilege, confidentiality agreements, and common or statutory law. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of this email or any attachment is prohibited. If you have received this email in error, please notify us at once by returning it to the sender and then deleting this copy from your system. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Colleen Forster via Histonet Sent: May 22, 2025 4:43 PM To: Gudrun Lang ; 'Colleen Forster' ; Bernice Frederick Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Bernice, I am aware of this. That is the question. If in fact, you need that solution to transform the Alcian Blue into a permanent state, how can it work with protocols that don't require that step? I used the Carson protocol because I was trying several to find one that kept the blue. But I want to understand the "why" here. When you are doing these 100 slides at a time you need it to be reliable and I want to understand. Colleen Forster ________________________________ From: Bernice Frederick via Histonet Sent: Thursday, May 22, 2025 2:22 PM To: Gudrun Lang ; 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions [Caution: This email originated from outside the organization.] I am using the Cancer Diagnostics kit with no issues and it does not use alkaline alcohol at all. Even other procedures I've see do not either. There is one in one of the Freida Carson books that should tell you everything you need to know. Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, May 22, 2025 12:59 PM To: 'Colleen Forster' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Pentachrome stain questions Hi Colleen, can you give us more details of the pentrachrome stain you use? Maybe there is a better chance for help. Gudrun -----Urspr?ngliche Nachricht----- Von: Colleen Forster via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 21. Mai 2025 18:51 An: histonet-request Betreff: [Histonet] Pentachrome stain questions Ok special stains guru's, I have a couple questions about reagents for the Pentachrome stain. Some protocols call for heated alkaline alcohol, some call for this solution at room temp. and some protocols don't use this solution at all. Can some please help me understand these 3 questions: 1. What is the purpose of this solution? 2. Is heat only to accelerate the process or necessary? 3. How can other protocols not use it at all? I have been reading papers, surfing the net trying to get a straight understanding. Any help to shed light would be greatly appreciated~ -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!RqZRyUlHzLCOCfk-NTRLYPRM-k4K5ESqu5VOf2mStFyiRGrtaiRnqsuBYwzZq8XJ3eRy5z-eiojwpb5_kNkyh7NJ_HfUpQDHUK_bNcUj$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIAL: This email (and any attachment) is confidential and may be protected by legal privilege, confidentiality agreements, and common or statutory law. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of this email or any attachment is prohibited. If you have received this email in error, please notify us at once by returning it to the sender and then deleting this copy from your system. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From elaineahoffman55 at yahoo.com Fri May 23 13:54:48 2025 From: elaineahoffman55 at yahoo.com (Elaine allison Hoffman) Date: Fri, 23 May 2025 18:54:48 +0000 (UTC) Subject: [Histonet] Removed from receiving emails References: <441492075.458142.1748026488582.ref@mail.yahoo.com> Message-ID: <441492075.458142.1748026488582@mail.yahoo.com> To whom it may concern: Wish to be removed from the Histology email list. I am no longer working in the laboratory field. I?m retired now. Thank you, Elaine A. Hoffman? Sent from Yahoo Mail for iPhone From jack.gyger at roche.com Wed May 28 08:25:15 2025 From: jack.gyger at roche.com (Gyger, Jack) Date: Wed, 28 May 2025 09:25:15 -0400 Subject: [Histonet] Histonet Digest, Vol 258, Issue 11 In-Reply-To: References: Message-ID: Please remove me from the Histology email list. I am no longer working in the laboratory field. On Sat, May 24, 2025 at 1:00?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Removed from receiving emails (Elaine allison Hoffman) > > > > ---------- Forwarded message ---------- > From: Elaine allison Hoffman > To: "Histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Fri, 23 May 2025 18:54:48 +0000 (UTC) > Subject: [Histonet] Removed from receiving emails > > > To whom it may concern: > Wish to be removed from the Histology email list. I am no longer working > in the laboratory field. I?m retired now. > Thank you, > Elaine A. Hoffman > Sent from Yahoo Mail for iPhone > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbireir at yahoo.com Thu May 29 03:09:19 2025 From: mbireir at yahoo.com (MANAHIL EL BIREIR) Date: Thu, 29 May 2025 12:09:19 +0400 Subject: [Histonet] Bone marrow trephine decalcification References: <20856ABC-9576-4A50-BE47-C0F51CB4EA49.ref@yahoo.com> Message-ID: <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> I hope this email finds you well. I am reaching out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results. To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis. Kind regards, Manahil Sent from my iPhone From VKurth at uwhealth.org Thu May 29 10:56:17 2025 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Thu, 29 May 2025 15:56:17 +0000 Subject: [Histonet] Merkel cell polyomavirus Message-ID: Hello Is anyone running the Merkel cell Polyomavirus ( CM2B4) on an Ultra? Thanks. Ginny Kurth, HT (ASCP) Developmental Specialist UW Health Surgical Pathology From georgina.gibson at merckgroup.com Thu May 29 22:02:52 2025 From: georgina.gibson at merckgroup.com (Georgina Gibson) Date: Fri, 30 May 2025 03:02:52 +0000 Subject: [Histonet] Bone marrow trephine decalcification In-Reply-To: <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> References: <20856ABC-9576-4A50-BE47-C0F51CB4EA49.ref@yahoo.com> <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> Message-ID: Hi Manahil, I look after our pathology products for Merck Life Science. We have a great gentle ready to use decal (EDTA based) many people use for Bone Marrow Trephines which gives good results for when need to do molecular work. It is one of our bestselling products in Australia. The bone marrow floats to the top once reaches the end point too. I am not sure if allowed to send product information here, please feel free to email me directly and I can give you more info if interested. Regards Georgina Gibson Field Application Specialist ? Tissue Diagnostics and Flavours & Fragrances Life Science | Solution Scientists [Image] Merck Life Science Pty Ltd | Ground Floor, Building 1 | 885 Mountain Highway | Bayswater | VIC 3153 | Australia Mobile: +61 (0)438 201 101 | E-mail: georgina.gibson at merckgroup.com | Web: www.merck.com.au Work days: Monday - Wednesday Orders: Phone (AU): 1800 800 097 | E-mail: CustomerSupport.ANZ at merckgroup.com | Online: www.SigmaAldrich.com Experience our products at www.sigmaaldrich.com ________________________________ From: MANAHIL EL BIREIR via Histonet Sent: Thursday, May 29, 2025 6:09:19 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bone marrow trephine decalcification I hope this email finds you well. I am reaching out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results. To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis. Kind regards, Manahil Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Eu8ikxSnpXkBCg!bZ777y6OglxSGhNt9PKu-ICop_JLeIS5gEFBSy0CaQoVNfje6NKXucoJ37oxeRGzr0rUiOn5CCZhO8Foa6htpTDW4qCxVGH6fPCO8h4$ This message and any attachment are confidential and may be privileged or otherwise protected from disclosure. If you are not the intended recipient, you must not copy this message or attachment or disclose the contents to any other person. If you have received this transmission in error, please notify the sender immediately and delete the message and any attachment from your system. Merck KGaA, Darmstadt, Germany and any of its subsidiaries do not accept liability for any omissions or errors in this message which may arise as a result of E-Mail-transmission or for damages resulting from any unauthorized changes of the content of this message and any attachment thereto. Merck KGaA, Darmstadt, Germany and any of its subsidiaries do not guarantee that this message is free of viruses and does not accept liability for any damages caused by any virus transmitted therewith. Click merckgroup.com/disclaimer to access the German, French, Spanish, Portuguese, Turkish, Polish and Slovak versions of this disclaimer. Please find our Privacy Statement information by clicking here: merckgroup.com/privacy-statements-by-location From rcartun at sbcglobal.net Thu May 29 22:03:01 2025 From: rcartun at sbcglobal.net (richard cartun) Date: Fri, 30 May 2025 03:03:01 +0000 (UTC) Subject: [Histonet] Bone marrow trephine decalcification In-Reply-To: <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> References: <20856ABC-9576-4A50-BE47-C0F51CB4EA49.ref@yahoo.com> <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> Message-ID: <1181859193.563792.1748574181904@mail.yahoo.com> Correct ...... you cannot use acid decal if you want to do molecular testing.? We switched to EDTA for small biopsy specimens that needed decalcifying prior to processing.? It is also important that the specimen be adequately fixed before decalcification.? I am no longer at Hartford Hospital, but I will try to find out where they purchase their EDTA now.? Note that you cannot use EDTA for large specimens (e.g., femoral head) because it takes way too long.? You can also carefully separate all the soft tissue fragments from bone, and process them separately without decalcification. Richard Cartun On Thursday, May 29, 2025 at 04:10:03 AM EDT, MANAHIL EL BIREIR via Histonet wrote: I hope this email finds you well. I am reaching? out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results. To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis. Kind regards, Manahil Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Fri May 30 02:43:45 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 30 May 2025 09:43:45 +0200 Subject: [Histonet] Bone marrow trephine decalcification In-Reply-To: <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> References: <20856ABC-9576-4A50-BE47-C0F51CB4EA49.ref@yahoo.com> <20856ABC-9576-4A50-BE47-C0F51CB4EA49@yahoo.com> Message-ID: <000001dbd136$9345b5a0$b9d120e0$@gmx.at> Hi! For "neutral" decalcification you can mix an 10-20% EDTA solution and add 4% NaOH to a pH about 7,1-7,4. There is no difference, if you use the salt or the acid, as long as the pH is checked. We fix 3mm-thick bone trephines over night in 4% NBF, then put them for about 30 hours (8 am one day to 3 pm next day afternoon) in 20% EDTA (pH 7,2). We let it sit on a magnetic stirrer with 40?C to enhance decalcification. After rinsing in tapwater the cassettes go into paraffin-processing. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: MANAHIL EL BIREIR via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 29. Mai 2025 10:09 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Bone marrow trephine decalcification I hope this email finds you well. I am reaching out regarding the optimisation of our bone marrow trephine specimen decalcification process. Currently, we are utilizing a ready-to-use acid rapid decalcification method. However, our Molecular Department has observed that the acid affects the quality of molecular results. To enhance our process and ensure optimal molecular sequencing outcomes, we would greatly appreciate it if you could share your bone marrow trephine decalcification protocol with us. Additionally, which decalcification reagent you use to mitigate potential adverse effects on molecular analysis. Kind regards, Manahil Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet