From relia1 at earthlink.net Mon Mar 3 11:22:25 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 3 Mar 2025 12:22:25 -0500 Subject: [Histonet] =?utf-8?q?=F0=9F=8C=9F_ICYMI!!_Celebrate_Histotechnolo?= =?utf-8?q?gy_Professionals_Day_=F0=9F=8C=9F?= Message-ID: <002001db8c60$d67df690$8379e3b0$@earthlink.net> ? ICYMI!! Celebrate Histotechnology Professionals Day ? March 10th marks the 15th anniversary of Histotechnology Professionals Day! ? In honor of our dedicated histotech professionals, we've curated a list of must-have resources to support and inspire you all year long. ? ? Check them out here: https://reliasolutionspambarker.wordpress.com/2025/02/26/celebrate-histotech nology-professionals-day-on-march-10th-with-this-must-have-resource-list Let's celebrate your hard work, dedication, and amazing contributions to science and medicine! Cheers to many more years of innovation and excellence in histotechnology! ? #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologycareers #histologyjobs #hpd ? Don't forget to share and spread the love! ? Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From relia1 at earthlink.net Mon Mar 3 11:22:28 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 3 Mar 2025 12:22:28 -0500 Subject: [Histonet] =?utf-8?q?=F0=9F=8C=9F_ICYMI!!_Celebrate_Histotechnolo?= =?utf-8?q?gy_Professionals_Day_=F0=9F=8C=9F?= Message-ID: <002401db8c60$d733b200$859b1600$@earthlink.net> ? ICYMI!! Celebrate Histotechnology Professionals Day ? March 10th marks the 15th anniversary of Histotechnology Professionals Day! ? In honor of our dedicated histotech professionals, we've curated a list of must-have resources to support and inspire you all year long. ? ? Check them out here: https://reliasolutionspambarker.wordpress.com/2025/02/26/celebrate-histotech nology-professionals-day-on-march-10th-with-this-must-have-resource-list Let's celebrate your hard work, dedication, and amazing contributions to science and medicine! Cheers to many more years of innovation and excellence in histotechnology! ? #ilovemyhistopeeps #histologyiscool #histologycareers #histologyjobs #hpd ? Don't forget to share and spread the love! ? ? ICYMI!! Celebrate Histotechnology Professionals Day ? : https://reliasolutionspambarker.wordpress.com/2025/02/26/celebrate-histotech nology-professionals-day-on-march-10th-with-this-must-have-resource-list Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From relia1 at earthlink.net Tue Mar 4 11:40:53 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 4 Mar 2025 12:40:53 -0500 Subject: [Histonet] Happy Mardi Gras Histonetters! ICYMI here's the link to our HPD Resource Page and Some HOT JOBS!! Message-ID: <000201db8d2c$946adb70$bd409250$@earthlink.net> Dear Histonetters, ? Happy Mardi Gras Histopeeps! ? I hope you?re having a Fantastic Day!! ? ICYMI here?s the link to RELIA?s Histotechnology Professionals Day Resource Page: Celebrate Histotechnology Professionals Day on March 10th with This Must-Have Resource List! | Histopeep Huddle ? ? I Have Some Amazing Opportunities! ? Explore roles in: Immunohistochemistry/Dermpath/GI/CancerDX and Leadership roles! ? Locations include: * Arizona ?? * California ? * Washington ? * Tennessee ? * Kentucky ? * Virginia ?? * North Carolina ?? * Florida ?? * And MORE! ? All of these opportunities are full-time and permanent. They offer excellent compensation, including bonuses up to $20K and relocation assistance. ? ? For more information, contact me! ? Direct Message me on Social Media Cell/Text: 407-353-5070 Email: relia1 at earthlink.net Let's celebrate Mardi Gras together and explore these fantastic opportunities! ?? Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From Nancy.Stedman at buschgardens.com Wed Mar 5 07:39:48 2025 From: Nancy.Stedman at buschgardens.com (Stedman, Nancy) Date: Wed, 5 Mar 2025 13:39:48 +0000 Subject: [Histonet] Any thoughts on mesh vs. paper biopsy bags? Message-ID: Hi Histonetters! Does anyone have any thoughts on nylon mesh vs. paper biopsy bags, specifically ease of use and durability? The paper bags seem like a cheaper and more environmentally friendly alternative to nylon.. are there any downsides? Thank you - Nancy Stedman From tford at wallisderm.com Wed Mar 5 11:06:22 2025 From: tford at wallisderm.com (Tracie Ford) Date: Wed, 5 Mar 2025 11:06:22 -0600 Subject: [Histonet] Balsam Bottle Message-ID: Hello, Just checking to see if anyone has an old Basam Bottle they are not using anymore and is looking to get rid of it? I am willing to purchase. I have a new histotech that loves to coverslip out of this bottle and I have been unable to locate her one. Thank you, Tracie Ford, HT (ASCP) Laboratory Supervisor Wallis Dermatology Associates 3535 N. 4th Street Suite 400 Longview, Texas 75605 Lab 903-918-8004 Fax 903-663-2454 Cell 903-235-9269 From tbraud at redeemerhealth.org Wed Mar 5 13:03:13 2025 From: tbraud at redeemerhealth.org (Terri Braud) Date: Wed, 5 Mar 2025 19:03:13 +0000 Subject: [Histonet] Balsam Bottle Message-ID: Balsam bottles can be bought directly from EMS or Fisher. I sent you the order info directly. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, March 5, 2025 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 256, Issue 3 CAUTION: This is an external email. Stop and think before clicking links or opening attachments. If you have questions or suspicions, call IT Support Center 215-938-3900 or ext. 4HELP. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Any thoughts on mesh vs. paper biopsy bags? (Stedman, Nancy) 2. Balsam Bottle (Tracie Ford) ---------------------------------------------------------------------- Message: 1 Date: Wed, 5 Mar 2025 13:39:48 +0000 From: "Stedman, Nancy" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Any thoughts on mesh vs. paper biopsy bags? Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Histonetters! Does anyone have any thoughts on nylon mesh vs. paper biopsy bags, specifically ease of use and durability? The paper bags seem like a cheaper and more environmentally friendly alternative to nylon.. are there any downsides? Thank you - Nancy Stedman ------------------------------ Message: 2 Date: Wed, 5 Mar 2025 11:06:22 -0600 From: Tracie Ford To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Balsam Bottle Message-ID: Content-Type: text/plain; charset="UTF-8" Hello, Just checking to see if anyone has an old Basam Bottle they are not using anymore and is looking to get rid of it? I am willing to purchase. I have a new histotech that loves to coverslip out of this bottle and I have been unable to locate her one. Thank you, Tracie Ford, HT (ASCP) Laboratory Supervisor Wallis Dermatology Associates 3535 N. 4th Street Suite 400 Longview, Texas 75605 Lab 903-918-8004 Fax 903-663-2454 Cell 903-235-9269 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 256, Issue 3 **************************************** ***Please Note: Redeemer Health has now changed its email domain from @holyredeemer . com to @redeemerhealth . org. Please alert your IT/cybersecurity team to ensure our new email domain is safe-listed.*** This email, and any files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. If you are not the intended recipient, please telephone or email the sender and delete this message and any attachment from your system; you must not copy or disclose the contents of this message or any attachment to any other person. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Redeemer Health. Redeemer Health may monitor email traffic data. From amyleehisto779 at gmail.com Fri Mar 7 19:34:20 2025 From: amyleehisto779 at gmail.com (Amy Lee) Date: Fri, 7 Mar 2025 17:34:20 -0800 Subject: [Histonet] LIMS Message-ID: Hello, Could anyone recommend a LIMS for a histology lab? The ratio of samples we received from clinical and research is about 7:3. The major workflow we are looking at is accession, microtomy, staining, imaging, archiving, delivery, reporting. If you have a user-friendly LIMS, please recommend. Thanks in advance. Amy From plucas at biopath.org Mon Mar 10 10:52:38 2025 From: plucas at biopath.org (Paula) Date: Mon, 10 Mar 2025 08:52:38 -0700 Subject: [Histonet] Prisma stainer freezing Message-ID: <007d01db91d4$741418d0$5c3c4a70$@biopath.org> Hi everyone, Has anyone had issues with the Sakura Prisma stainer "freezing" during a program? Ours is not on a service contract, but we do have an outside company that PM's our machine, and he is going to look into it. We have to turn it off, then on but then all the programs are wiped out, so the baskets are left in their remaining buckets to finish off manually. I just want to see if anyone had issues like this. Thank you in advance, Paula From john at imebinc.com Mon Mar 10 12:15:51 2025 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Mon, 10 Mar 2025 10:15:51 -0700 Subject: [Histonet] Histonet Digest, Vol 256, Issue 6 In-Reply-To: References: Message-ID: <571D38F0-D911-4747-8788-F8BC728786AD@imebinc.com> Sakura prisma stainer needs a new mother board Not sure if Sakura sells one IMEB Inc > On Mar 10, 2025, at 10:12?AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Prisma stainer freezing (Paula) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 10 Mar 2025 08:52:38 -0700 > From: "Paula" > To: > Subject: [Histonet] Prisma stainer freezing > Message-ID: <007d01db91d4$741418d0$5c3c4a70$@biopath.org> > Content-Type: text/plain; charset="us-ascii" > > Hi everyone, > > > > Has anyone had issues with the Sakura Prisma stainer "freezing" during a > program? Ours is not on a service contract, but we do have an outside > company that PM's our machine, and he is going to look into it. We have to > turn it off, then on but then all the programs are wiped out, so the baskets > are left in their remaining buckets to finish off manually. > > > > I just want to see if anyone had issues like this. > > > > Thank you in advance, > > Paula > > > > > > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 256, Issue 6 > **************************************** From kilosos2 at aol.com Mon Mar 10 12:43:17 2025 From: kilosos2 at aol.com (kilosos2 at aol.com) Date: Mon, 10 Mar 2025 17:43:17 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 256, Issue 6 In-Reply-To: References: Message-ID: <1921705427.1252567.1741628597081@mail.yahoo.com> The stainer is is Windows 2000. Maybe some files or the hard drive it may have is corrupt? Does it stop randomly or in certain positions? On Monday, March 10, 2025 at 01:00:10 PM EDT, histonet-request at lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Prisma stainer freezing (Paula) ---------------------------------------------------------------------- Message: 1 Date: Mon, 10 Mar 2025 08:52:38 -0700 From: "Paula" To: Subject: [Histonet] Prisma stainer freezing Message-ID: <007d01db91d4$741418d0$5c3c4a70$@biopath.org> Content-Type: text/plain;??? charset="us-ascii" Hi everyone, Has anyone had issues with the Sakura Prisma stainer "freezing" during a program?? Ours is not on a service contract, but we do have an outside company that PM's our machine, and he is going to look into it.? We have to turn it off, then on but then all the programs are wiped out, so the baskets are left in their remaining buckets to finish off manually. I just want to see if anyone had issues like this. Thank you in advance, Paula ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 256, Issue 6 **************************************** From john at imebinc.com Mon Mar 10 13:41:54 2025 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Mon, 10 Mar 2025 11:41:54 -0700 Subject: [Histonet] Validation of parriffin Message-ID: <283996C9-C4B9-4AEE-B697-1F4423676E9C@imebinc.com> Histonetters ?When a new paraffin is introduced to a Tissue processor is validation required? If so what is protocol? Thank you IMEB Inc From Donna.Willis at BSWHealth.org Mon Mar 10 14:29:38 2025 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Mon, 10 Mar 2025 19:29:38 +0000 Subject: [Histonet] Validation of parriffin In-Reply-To: <283996C9-C4B9-4AEE-B697-1F4423676E9C@imebinc.com> References: <283996C9-C4B9-4AEE-B697-1F4423676E9C@imebinc.com> Message-ID: Yes. That would be a process change. You also have to re-validate IHC. Donna Willis HT/HTL (ASCP) Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 1st Floor Roberts Building 3500 Gaston Ave. Dallas, Texas 75246 214-820-2465 office 214-725-6184 cell -----Original Message----- From: John O?Brien via Histonet Sent: Monday, March 10, 2025 1:42 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Validation of parriffin CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Histonetters ?When a new paraffin is introduced to a Tissue processor is validation required? If so what is protocol? Thank you IMEB Inc _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!HTroVlJ4ETZBdZ5WFcQxPDNHEIf2uMMQ8bEB-GPKb11SIchj7JEt22uNPeELfLjTRHYaFpQwJnY7eHdV9C_ZcsOZaM45ADTVSg7ooA$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From gu.lang at gmx.at Tue Mar 11 01:54:07 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 11 Mar 2025 07:54:07 +0100 Subject: [Histonet] sources for IHC-results in normal tissue Message-ID: <000301db9252$63437360$29ca5a20$@gmx.at> Dear histonetters! I am looking for additional sources for IHC-results in normal tissues with detailed descriptions. It should serve for learning about high, low and non-expressors in normal tissues. As far I use the informations on NordiQC, the Agilent Atlas of Controls and partly the protein atlas. Does anyone know further reliable and available sources? Thanks in advance and kind regards Gudrun Lang Gudrun Lang Landgutstra?e 25 4040 Linz From afhenwood at outlook.com Fri Mar 14 02:23:35 2025 From: afhenwood at outlook.com (Tony Henwood) Date: Fri, 14 Mar 2025 07:23:35 +0000 Subject: [Histonet] Validation of parriffin In-Reply-To: <283996C9-C4B9-4AEE-B697-1F4423676E9C@imebinc.com> References: <283996C9-C4B9-4AEE-B697-1F4423676E9C@imebinc.com> Message-ID: Hi John, Following is the process we used when a change in wax was needed (different supplier, change in formulation etc). Validation of tissue processing waxes Changing processing and embedding waxes could improve or badly affect your histology quality. Your lab has years of experience with a particular wax and have developed and optimised stains (including immunohistochemistry) on tissues embedded in this wax. To change requires re-validation of all these stains. 1. Compare data sheet with old wax datasheet (melting point, additives). 2. Wax Purity Test If components of the wax are not soluble in the clearing agent, then tissue samples will not be effectively infiltrated during processing nor will they dewax adequately for staining methods. The following test procedure has been recommended: 1. Take 1 gram of wax and mix in 20ml of xylene 2. Record time for full dissolution 3. Check for any undissolved residues and contaminants 1. Occurrence of nuclear birefringence Incomplete wax removal can cause several problems including birefringence of cell nuclei (Vlachos 1968, Stain Technol. 43: 89?95), so-called pink disease in which the distribution of stains is patchy and distinct nuclear margins are lost, and weak to false negative immunohistochemical staining (Henwood 2012, Biotechnic & Histochemistry, 87(1), 46-50). Although there may be several causes of pink disease, one common cause is the incomplete removal of wax. It is commonly accepted that birefringent cell nuclei are due to incomplete wax removal by xylene (Vlachos 1968). Vlachos (1968) also noted that nuclear staining was weaker in areas that showed increased birefringence. Using routine dewaxing and rehydration methods followed by observation using polarisation microscopy can indicate differences in wax properties that might require changes in dewaxing procedures prior to routine staining. 1. Tissue Wax Test It is important to ensure that use of the wax results in well-processed blocks that are easy to section. Whenever a new wax is being assessed it is important that several tissues are used. I would recommend: * Brain ? a tissue rich in lipids and delicate neural fibres, can 6-7?m sections be easily sectioned? * Liver ? regular polyhedral cells bound in cords, that will show any variation in infiltration and possible shrinkage across the tissue * Skin ? a mix of keratinised squamous cells, elastic and connective tissue * Tonsil or lymph node ? how do the lymphoid cells appear especially when thin 2?m sections are cut? * Appendix ? a mix of delicate epithelial cells, collagen and muscle. Do stained sections exhibit folding of the epithelial cells due to excessive shrinkage? * Kidney ? what is the quality of 2?m sections? 1. It is important to compare the new wax with the old (ie run the tests in parallel). Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: John O?Brien via Histonet Sent: 11 March 2025 07:41 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Validation of parriffin Histonetters ?When a new paraffin is introduced to a Tissue processor is validation required? If so what is protocol? Thank you IMEB Inc _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Fri Mar 14 11:05:59 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 14 Mar 2025 12:05:59 -0400 Subject: [Histonet] Early Bird Gets The Worm! Message-ID: <000c01db94fa$faf3d420$f0db7c60$@earthlink.net> Hey Histopeeps! Early bird gets the worm and the resume tune -up! It?s that time again starting Monday Free Resume Tune-ups but you can get in first! Just shoot me an email to relia1 at earthlink.net and we can get started! Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From blayjorge at gmail.com Sat Mar 22 21:37:21 2025 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Sat, 22 Mar 2025 22:37:21 -0400 Subject: [Histonet] Protocols for differential staining of secretory and nervous tissues on insects for light and transmission electron microscopy Message-ID: Hello: I am looking for successful protocols to differentially stain secretory and nervous tissues on insects for light and transmission electron microscopy. If anyone knows of such protocols, please, feel free to contact me off the list. Thanks. Sincerely, *Jorge* From tcampbell at fgamd.com Fri Mar 28 06:23:49 2025 From: tcampbell at fgamd.com (Tasha Campbell) Date: Fri, 28 Mar 2025 11:23:49 +0000 Subject: [Histonet] Part Time Help Frederick MD Message-ID: Hello all, I run a GI lab in Frederick MD and need a histotech that would be able to work afternoons/evenings for a few hours each day to embed/cut/stain. If anyone is interested, send me an email with your resume. Thank you!!!! Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 Phone: 301-695-6800 ext. 144 Fax: 301-695-6891 From carl.hobbs at kcl.ac.uk Sat Mar 29 12:51:40 2025 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 29 Mar 2025 17:51:40 +0000 Subject: [Histonet] Uploading images to Histonet Image archive Message-ID: Hi? I have been uploading my images ( histology ) to Histonet Images for years I cannot now access it, using the same link I get this message: "Sorry, you have been blocked You are unable to access histosearch.com" I'd be interested to know why If they have stopped doing this, I assume that all my images have been deleted? If so, is there another Histology site that allows image uploads? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From John.Shelton at UTSouthwestern.edu Sun Mar 30 08:56:11 2025 From: John.Shelton at UTSouthwestern.edu (John Shelton) Date: Sun, 30 Mar 2025 13:56:11 +0000 Subject: [Histonet] Uploading images to Histonet Image archive In-Reply-To: References: Message-ID: Howdy Carl and all Histonet Subscribers, The Image Archive that some of you utilized and affectionately associated with Histonet was a functional offering of Histosearch.com, a commercial site hosted from private servers in Florida, USA, and not a part of Histonet. Histoserach.com mined Histonet?s post-archive and mirrored it, thereby presenting an outward feel and association with Histonet. Similarly, Histosearch.com hosted links to UT Southwestern?s Histonet subscription page and UT Southwestern?s Histonet Ownership page, furthering the presentation of a formal relationship. As you may have gleaned from the incorporation of past participle in the preceding description, the content on Histosearch.com is no longer functional, with the domain?s owners allowing it to go dark. We are saddened about the loss of image sharing functionality, and saddened that the loss is now being associated with Histonet. As we at UT Southwestern move forward with challenges that face Histonet, an image sharing archive is on the wishlist. On a personal note, Carl, we all thank you for your dedicated contributions to the global histology community. Your words of wisdom and posts on your personal bench practices are appreciated; they have helped a great many. John M Shelton Senior Database Analyst Histonet List Owner & Moderator UT Southwestern Histo Pathology Core 5323 Harry Hines Blvd Dallas, Texas 75390-8573 (214)929-6264 Visit the Core's website at: https://www.utsouthwestern.edu/research/core-facilities/histo-pathology/ Visit Histonet Archives at: http://lists.utsouthwestern.edu/pipermail/histonet/ ________________________________ From: zzz_Tag_histonet-lists Sent: Saturday, March 29, 2025 12:51 PM To: zzz_Tag_histonet-lists Subject: [Histonet] Uploading images to Histonet Image archive Hi I have been uploading my images ( histology ) to Histonet Images for years I cannot now access it, using the same link I get this message: "Sorry, you have been blocked You are unable to access histosearch.com" I'd be interested to know why If they have stopped doing this, I assume that all my images have been deleted? If so, is there another Histology site that allows image uploads? Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ UT Southwestern Medical Center The future of medicine, today. From vavalos at dermatologyaz.com Mon Mar 31 13:17:27 2025 From: vavalos at dermatologyaz.com (Vanessa Avalos) Date: Mon, 31 Mar 2025 18:17:27 +0000 Subject: [Histonet] Competency Policies & Procedures Message-ID: We are working on updating our Procedure Manual. Would anyone out there be willing to share their competency policy & evaluation form for techs and for performing KOH tests? Looking for ideas on creating one tailored to our practice. Thank you, V. Avalos AD HISTO