From relia1 at earthlink.net Mon Jun 2 09:32:23 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 2 Jun 2025 10:32:23 -0400 Subject: [Histonet] ICYMI! A Quick Way to Help Your Lab and Earn a Bonus! Plus Exciting Opportunities!! Message-ID: <008701dbd3cb$2994c8c0$7cbe5a40$@earthlink.net> Happy Friday Histopeeps!! Are things feeling a little hectic in your lab lately? * Concerned About YOUR Time Off Because Of Short Staffing? * Could Your Team Use Some Extra Support? The Kind That Sticks Around And Is Not Gone In 13 Weeks? I?d Love To Step In?And There?s An Exciting Opportunity For You, Too! By referring me to your supervisor, manager, or director, you could help fill a critical role in your lab AND earn a referral bonus. Here?s how it works: Simply email me at relia1 at earthlink.net with the name and email address of your supervisor, manager, or director. If I secure the job and place someone in the position, you?ll receive a referral bonus just like referring a candidate! Histopeeps!! You Can Let Your Supervisor Know You?re Referring Me OR Keep It Confidential. Whatever You?re Comfortable with Works For Me! Let?s work together to help your team thrive. Feel free to reach out anytime?I?m always here to help! Looking for a new opportunity or simply curious about what's out there? Right now, I?m actively working on exciting roles, including: ? Histology Leadership Roles ? HT/HTL roles across all histology modalities ? Entry-level opportunities for new grads My clients are offering competitive compensation, relocation assistance, sign-on bonuses, and an outstanding work environment?these are full-time, permanent positions in some of the best locations across the U.S. Want more details? Just a little curious? Shoot me an email at relia1 at earthlink.net or call/text me at 407-353-5070?I?d be happy to answer any questions you have!? Wishing YOU A Fantastic Day! Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From melissa at alliedsearchpartners.com Thu Jun 5 10:04:09 2025 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Thu, 5 Jun 2025 15:04:09 +0000 Subject: [Histonet] Temp Histotech Jobs Each Corner of the US-FL & WA Message-ID: Hello- I am seeking Histotechs who are interested in working the below mentioned temporary assignments. Can discuss details directly if interested and available. Please respond to this email if you have any questions and interest. Seattle WA Area: 2 months assignment with 1 month extension possible at the end depending on the needs. Tues-Sat 5a-1:30pm shfit. Willing to take a part time schedule on the busiest days-Thu/Fri/Sat or someone that can only work 1 or a couple of those days. Boca Raton Area: 3 month assignment. Mon-Fri 3p-11:30p. Need to be strong in embeddign and cutting. Let me know if interested we can discuss in further detail! From relia1 at earthlink.net Thu Jun 5 12:56:21 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 5 Jun 2025 13:56:21 -0400 Subject: [Histonet] Sun Soaked Opportunities in Florida! Ready for the Beachy Lifestyle? Message-ID: <01eb01dbd643$25e9bdf0$71bd39d0$@earthlink.net> ?? Sun-Soaked Career Opportunities in Florida! ? Hey #Histopeeps! Ready to take your career to paradise? I?ve got exciting full-time positions along Florida?s stunning coastlines! ? ? Leadership, Routine Histology & Specialty Roles ? Top-tier companies offering great pay & benefits ? Relocation & sign-on bonuses available! ? Work hard, then unwind on Florida?s beautiful beaches! ? Reach out for details! DM me or Email: mailto:relia1 at earthlink.net or cell/text: 407-353-5070 Let?s get you into your dream job. ? #Histology #FloridaJobs #CareerMoves #ilovemyhistopeeps #jobs4myhistopeeps #histologyjobs #histologycareers Thanks -Pam From relia1 at earthlink.net Thu Jun 5 12:56:21 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 5 Jun 2025 13:56:21 -0400 Subject: [Histonet] =?utf-8?q?My_Latest_Blog_Post_is_UP!__Who=E2=80=99s_In?= =?utf-8?q?terviewing_Who=3F_A_Guide_for_Healthcare_Hiring_Managers?= =?utf-8?q?=2E?= Message-ID: <01ef01dbd643$26351ed0$729f5c70$@earthlink.net> Dear Histonetters, I hope this message finds you well! I want to share my latest blog post: Who?s Interviewing Who? A Guide for Healthcare Hiring Managers. Hiring in healthcare is evolving! Interviews aren?t just assessments?top candidates are evaluating your workplace, culture, and leadership just as much as you?re assessing their skills. ? How can you attract top healthcare talent? **Create a welcoming environment. **Communicate your mission & growth opportunities. **Be transparent about the role. Want to stand out in today?s job market? Don?t miss our latest guide! Read now: https://www.linkedin.com/pulse/whos-interviewing-who-guide-healthcare-hiring -pam-barker-7blec Here Is A List Of Locations Where Some Of My Best Candidates Are Now Or Want To Move To! ? Washington ? Colorado ? Texas ? North Carolina ? South Carolina ? Georgia ? Maryland ? Washington DC ? Tennessee These are the techs I have talked to just THIS WEEK! I chat with techs daily NATIONWIDE. I know what they want and where they want to be and when! Ready to gear up for Summer 2025 and Q4 2025 hiring? Contact Me! Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 Let me help you navigate the complexities of the histology job market and find the best candidates for your lab. Best regards, Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From gu.lang at gmx.at Mon Jun 9 03:26:43 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 9 Jun 2025 10:26:43 +0200 Subject: [Histonet] amount of nucleic acids in ffpet Message-ID: <000a01dbd918$3bd1cf90$b3756eb0$@gmx.at> Hi all! I came across this sentence in a histo-textbook from Baker 1958: "The amount of nucleic acids (DNA + RNA) in cells is reduced by 10 to 35% by formaldehyde fixation." I wonder, what it means. Do the nucleic acids get lost during fixation? Or is it just an expression for the lack of stainability or proof at that time? Maybe someone has an opinion to share? Kind regards Gudrun From blayjorge at gmail.com Mon Jun 9 07:47:51 2025 From: blayjorge at gmail.com (Jorge A. Santiago-Blay) Date: Mon, 9 Jun 2025 08:47:51 -0400 Subject: [Histonet] Stains that associate differentially to epithelial vs nervous tissue in arthropods, particularly insects Message-ID: Hello: I am looking for positive recommendations on stains that associate differentially to epithelial vs nervous tissue in arthropods, particularly insects. Any leads will be appreciated. Thanks. Sincerely, Jorge From relia1 at earthlink.net Wed Jun 11 12:54:02 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 11 Jun 2025 13:54:02 -0400 Subject: [Histonet] =?utf-8?b?8J+aqCBIaXN0b3RlY2hzIG9uIHRoZSBNb3Zl4oCUTGV0?= =?utf-8?b?4oCZcyBDb25uZWN0ISDwn5qo?= Message-ID: <00a101dbdaf9$dbe45dd0$93ad1970$@earthlink.net> ? Histotechs on the Move?Let?s Connect! ? Hey everyone! Hope your summer is off to a great start! ? The histotech shortage is real, but I?ve got amazing candidates ready to jump into permanent, full-time roles. They?re experienced, ASCP certified or eligible, and eager to make a move?whether it?s for relocation, a better schedule, career growth, or transitioning from travel gigs. ? What they bring to the table: ?? Solid references & great work ethic ?? Realistic salary expectations ?? Ready to start within 2?4 weeks Last time I shared a list like this, nearly every tech landed a job?so don?t miss out! ? These professionals are open to opportunities nationwide, from the Atlantic Coast to the Pacific and everywhere in between. If you?re hiring or just want to chat, hit me up at relia1 at earthlink.net or call 407-353-5070. I?d love to walk you through how my services work! Know someone looking? Tag them or share this post! ? "The secret of getting ahead is getting started." ? Mark Twain #HistotechJobs #HiringNow #CareerMoves #LabLife #Hiring #Histology #ilovemyhistopeeps #jobs4myhistopeeps #HistologyJobs Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From tcampbell at fgamd.com Fri Jun 13 06:57:04 2025 From: tcampbell at fgamd.com (Tasha Campbell) Date: Fri, 13 Jun 2025 11:57:04 +0000 Subject: [Histonet] Leica Slide Racks Message-ID: Hello everyone, I was wondering if anyone knows if they make the manual staining lines that are designed to fit the Leica glass coverslipper 30 slide racks. It is very frustrating that I do IHCs and special stains and to dehydrate the stains I have to put them in the 24 slide racks and then move them over to the leica racks to coverslip. I do have the Leica XL stainer and I have a dehydration program, but I cannot run that program and the H&E program at the same time so its useless basically. Thanks! Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 Phone: 301-695-6800 ext. 144 Fax: 301-695-6891 From durmtissuebank01 at gmail.com Fri Jun 13 17:09:36 2025 From: durmtissuebank01 at gmail.com (Ni Nie) Date: Fri, 13 Jun 2025 18:09:36 -0400 Subject: [Histonet] Expired Tissue Blocks Message-ID: Don't discard your expired tissue blocks after 10 years! They could be worth thousands of dollars to researchers worldwide. We will evaluate what you are about to throw away and either pay you cash if your laboratory policy allows, or we can arrange to provide your lab with an endless supply of consumables including microscope slides, slide folders and dozens of other items. Although our researchers often don't require detailed Path reports for each block we will pay a substantial premium if they are available. A simple spreadsheet of the diagnosis corresponding to the block number is enough. We cannot take them if they have a patient name or any other patient identifier. The courts in the United States have confirmed dozens of times repeatedly that these blocks are the property of your institution unless the patients directly request them. Why discard them when they can be valuable for medical researchers and your lab? Contact DR. Su Suyaun at durmtissuebank01 at gmail.com for details. From relia1 at earthlink.net Mon Jun 16 10:51:49 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 16 Jun 2025 11:51:49 -0400 Subject: [Histonet] =?utf-8?b?IEhpc3RvdGVjaHMgb24gdGhlIE1vdmXigJRMZXTigJlz?= =?utf-8?q?_Connect!?= Message-ID: <041501dbded6$93f00570$bbd01050$@earthlink.net> Histotechs on the Move?Let?s Connect! Hey everyone! Hope your summer is off to a great start! The histotech shortage is real, but I?ve got amazing candidates ready to jump into permanent, full-time roles. They?re experienced, ASCP certified or eligible, and eager to make a move?whether it?s for relocation, a better schedule, career growth, or transitioning from travel gigs. What they bring to the table: Solid references & great work ethic Realistic salary expectations Ready to start within 2?4 weeks Last time I shared a list like this, nearly every tech landed a job?so don?t miss out! These professionals are open to opportunities nationwide, from the Atlantic Coast to the Pacific and everywhere in between. If you?re hiring or just want to chat, hit me up at relia1 at earthlink.net or call 407-353-5070. I?d love to walk you through how my services work! Know someone looking for techs? share this post! Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From cmmathis at wakehealth.edu Mon Jun 16 13:30:45 2025 From: cmmathis at wakehealth.edu (Cathy M. Mathis/Comparative Medicine) Date: Mon, 16 Jun 2025 18:30:45 +0000 Subject: [Histonet] Formic Acid treatment for b-Amyloid staining Message-ID: Is there a reason to using formic acid treatment before beta amyloid staining in IHC?? I have only been using a high pH antigen retrieval solution and that works fine for me. Thank you for your time, Cathy Cathy M. Mathis Research Lab Technician IV Dept. of Pathology \ Comparative Pathology Lab Wake Forest University School of Medicine p 336.716.1538 cmmathis at wakehealth.edu www.wakehealth.edu ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From Valerie.Hannen at parrishmed.com Mon Jun 16 14:52:41 2025 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Mon, 16 Jun 2025 19:52:41 +0000 Subject: [Histonet] Release of Tissue Policy/ form Message-ID: Good Afternoon all!! I am hoping someone can share with me their policy and form that is used in accordance of the policy on the subject of releasing tissue per patient request to the patient? We have had a few incident recently where the patient/ family has asked for placenta and or the fetus ( less than 20 weeks gestation). My concern is that these specimens or any specimen in formalin is the formalin! We have a very old policy and I am not comfortable with it. I would like to use other's policies as a guide (not copy it verbatim). Any suggestions or help would be greatly appreciated. TIA. v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From rcartun at sbcglobal.net Mon Jun 16 19:20:13 2025 From: rcartun at sbcglobal.net (richard cartun) Date: Tue, 17 Jun 2025 00:20:13 +0000 (UTC) Subject: [Histonet] Release of Tissue Policy/ form In-Reply-To: References: Message-ID: <844138244.1233291.1750119613619@mail.yahoo.com> Hi, Valerie! I worked in Pathology at a hospital here in Connecticut for many years and handled all these requests, sometimes in conjunction with our Legal Department.? The State of Connecticut has a statute that states human tissue can only be handled by medical personnel in hospitals, surgery centers, labs, or funeral homes.? Therefore, we would only release patient specimens that were in formalin (mostly fetuses for burial) to a representative from a funeral home.? The patient or family member would have to contact the funeral home for pickup and pay for all associated charges.? We also had a release form that the requestor and the funeral home would have to complete and sign off on.? ? With the exception of a fetus for burial, these requirements almost always resulted in patients canceling their initial request.? I also found that speaking with the requester and educating them on this issue helped, too.? For example, many towns have ordinances that prohibit burying human tissue on the homeowner's property.?? For patients who wanted their "fresh" placenta after giving birth, our hospital policy was for the OB Department to handle it; Pathology did not get involved! I hope this information helps. Richard W. Cartun, MS, PhDMorphologic Proteomics, LLC On Monday, June 16, 2025 at 03:53:06 PM EDT, Hannen, Valerie via Histonet wrote: Good Afternoon all!! I am hoping someone can share with me their policy and form that is used in accordance of the policy on the subject of releasing tissue per patient request to the patient?? We have had a few incident recently where the patient/ family has asked for placenta and or the fetus ( less than 20 weeks gestation). My concern is that these specimens or any specimen in formalin is the formalin! We have a very old policy and I am not comfortable with it. I would like to use other's policies as a guide (not copy it verbatim).? Any suggestions or help would be greatly appreciated. TIA. v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333? Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bonnie.Whitaker at osumc.edu Mon Jun 16 19:22:07 2025 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Tue, 17 Jun 2025 00:22:07 +0000 Subject: [Histonet] Formic Acid treatment for b-Amyloid staining In-Reply-To: References: Message-ID: I used this years ago, before all the different retrieval buffers were readily available. I believe it was from a NovaCastra provided protocol, but I could be mistaken about that. Get Outlook for iOS ________________________________ From: Cathy M. Mathis/Comparative Medicine via Histonet Sent: Monday, June 16, 2025 2:30:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formic Acid treatment for b-Amyloid staining Is there a reason to using formic acid treatment before beta amyloid staining in IHC?? I have only been using a high pH antigen retrieval solution and that works fine for me. Thank you for your time, Cathy Cathy M. Mathis Research Lab Technician IV Dept. of Pathology \ Comparative Pathology Lab Wake Forest University School of Medicine p 336.716.1538 cmmathis at wakehealth.edu https://urldefense.com/v3/__http://www.wakehealth.edu__;!!AU3bcTlGKuA!BgMR6haspl_4gdQf833nb8mMORTdWzzfa2esu2-m8htjKWACWx0I_-SRc86hplyehfCBzhg4IViqQ7Ub_00gWjWxfmz0DggTmile4g$ ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!BgMR6haspl_4gdQf833nb8mMORTdWzzfa2esu2-m8htjKWACWx0I_-SRc86hplyehfCBzhg4IViqQ7Ub_00gWjWxfmz0Dgg-Yhmzsg$ From carl.hobbs at kcl.ac.uk Tue Jun 17 13:14:56 2025 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Tue, 17 Jun 2025 18:14:56 +0000 Subject: [Histonet] Formic Acid treatment for b-Amyloid staining Message-ID: Hi If HIER works best for you, stay with it It is good to do an initial comparison, imho I find that some anti A-beta abs work better after Formic acid AR, others better after Citric acid HIER Some anti A-beta abs also disclose intracellular APP after Citra HIER....never so after Formic acid ( eg: anti Amyloid beta 1-17 Abcam ab11132 will show amyloid plaques and intracellular APP after Citra HIER....after Formic acid AR, only amyloid plaques are seen) Have to admit to having included Congo red (Direct red 28) occasionally....stains the smallest deposits; espec. when including fluorescence examination Always most interested to read of your Xp, Cathy NB: I can't open Bonnie's link so, can't comment Best wishes Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From tbraud at redeemerhealth.org Tue Jun 17 15:13:59 2025 From: tbraud at redeemerhealth.org (Terri Braud) Date: Tue, 17 Jun 2025 20:13:59 +0000 Subject: [Histonet] Release of formalin fixed tissue Policy Message-ID: <3707cd3967ac452899755391a173434e@redeemerhealth.org> Here is our policy. The form that is signed for the release has a formalin and biohazard warning. No wet tissue is returned to a patient, but only released to a funeral home. Hope it helps PURPOSE: To define the types of specimens that may be returned to the patient upon request of the patient and/or physician, and to provide appropriate warnings via a signed form (AP-39 attached), to the patient and/or physician of possible biohazard and hazardous chemical exposure. POLICY: Specimens that may be released to patients are limited to: A. Gallstones B. Urinary or salivary calculi C. Foreign bodies D. Teeth E. Surgical Orthopedic Hardware Specimens that may be released for purposes of burial are as follows: A. Products of Conception (POC) to funeral director only B. Any other human tissue to funeral director only PROCEDURE: A. A request is received by lab for the return of a specimen to the patient. The request is either written on the histopathology form received with the specimen or by phone from the OR unit, Floor, patient or physician. B. The histology technician assigned to gross room is notified as well as the grossing pathologist. C. Obtain a Request for Return of Specimen to Patient form (AP-39) and complete required information. D. Prepare specimen as follows and attach form to bag to be read, signed by patient and/or physician and filed with surgical pathology report. E. The pathologist or designee retrieves the specimen and places it into a clean plastic container which is labeled with the patient's name and specimen accession number. F. Pour a sufficient amount of 100% alcohol over the specimen and agitate container to rinse off excess formalin. Pour off all 100% (discard alcohol in waste container). Attach lid and place container in paper bag. Label bag with patient and physician name. WARNING: All specimens must be formalin fixed prior to return to patient. (Hardware must be placed in formalin over night). Only those specimens listed above may be returned. No soft tissue will be returned. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, June 17, 2025 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 259, Issue 9 CAUTION: This is an external email. Stop and think before clicking links or opening attachments. If you have questions or suspicions, call IT Support Center 215-938-3900 or ext. 4HELP. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Formic Acid treatment for b-Amyloid staining (Cathy M. Mathis/Comparative Medicine) 2. Release of Tissue Policy/ form (Hannen, Valerie) 3. Re: Release of Tissue Policy/ form (richard cartun) 4. Re: Formic Acid treatment for b-Amyloid staining (Whitaker, Bonnie) ---------------------------------------------------------------------- Message: 1 Date: Mon, 16 Jun 2025 18:30:45 +0000 From: "Cathy M. Mathis/Comparative Medicine" To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Formic Acid treatment for b-Amyloid staining Message-ID: Content-Type: text/plain; charset="us-ascii" Is there a reason to using formic acid treatment before beta amyloid staining in IHC?? I have only been using a high pH antigen retrieval solution and that works fine for me. Thank you for your time, Cathy Cathy M. Mathis Research Lab Technician IV Dept. of Pathology \ Comparative Pathology Lab Wake Forest University School of Medicine p 336.716.1538 cmmathis at wakehealth.edu www.wakehealth.edu ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. ------------------------------ Message: 2 Date: Mon, 16 Jun 2025 19:52:41 +0000 From: "Hannen, Valerie" To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Release of Tissue Policy/ form Message-ID: Content-Type: text/plain; charset="us-ascii" Good Afternoon all!! I am hoping someone can share with me their policy and form that is used in accordance of the policy on the subject of releasing tissue per patient request to the patient? We have had a few incident recently where the patient/ family has asked for placenta and or the fetus ( less than 20 weeks gestation). My concern is that these specimens or any specimen in formalin is the formalin! We have a very old policy and I am not comfortable with it. I would like to use other's policies as a guide (not copy it verbatim). Any suggestions or help would be greatly appreciated. TIA. v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com ------------------------------ Message: 3 Date: Tue, 17 Jun 2025 00:20:13 +0000 (UTC) From: richard cartun To: "Histonet at lists.utsouthwestern.edu" , "Hannen, Valerie" Subject: Re: [Histonet] Release of Tissue Policy/ form Message-ID: <844138244.1233291.1750119613619 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi, Valerie! I worked in Pathology at a hospital here in Connecticut for many years and handled all these requests, sometimes in conjunction with our Legal Department.? The State of Connecticut has a statute that states human tissue can only be handled by medical personnel in hospitals, surgery centers, labs, or funeral homes.? Therefore, we would only release patient specimens that were in formalin (mostly fetuses for burial) to a representative from a funeral home.? The patient or family member would have to contact the funeral home for pickup and pay for all associated charges.? We also had a release form that the requestor and the funeral home would have to complete and sign off on.? ? With the exception of a fetus for burial, these requirements almost always resulted in patients canceling their initial request.? I also found that speaking with the requester and educating them on this issue helped, too.? For example, many towns have ordinances that prohibit burying human tissue on the homeowner's property.?? For patients who wanted their "fresh" placenta after giving birth, our hospital policy was for the OB Department to handle it; Pathology did not get involved! I hope this information helps. Richard W. Cartun, MS, PhDMorphologic Proteomics, LLC On Monday, June 16, 2025 at 03:53:06 PM EDT, Hannen, Valerie via Histonet wrote: Good Afternoon all!! I am hoping someone can share with me their policy and form that is used in accordance of the policy on the subject of releasing tissue per patient request to the patient?? We have had a few incident recently where the patient/ family has asked for placenta and or the fetus ( less than 20 weeks gestation). My concern is that these specimens or any specimen in formalin is the formalin! We have a very old policy and I am not comfortable with it. I would like to use other's policies as a guide (not copy it verbatim).? Any suggestions or help would be greatly appreciated. TIA. v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333? Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Tue, 17 Jun 2025 00:22:07 +0000 From: "Whitaker, Bonnie" To: "Cathy M. Mathis/Comparative Medicine" , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Formic Acid treatment for b-Amyloid staining Message-ID: Content-Type: text/plain; charset="us-ascii" I used this years ago, before all the different retrieval buffers were readily available. I believe it was from a NovaCastra provided protocol, but I could be mistaken about that. Get Outlook for iOS ________________________________ From: Cathy M. Mathis/Comparative Medicine via Histonet Sent: Monday, June 16, 2025 2:30:45 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Formic Acid treatment for b-Amyloid staining Is there a reason to using formic acid treatment before beta amyloid staining in IHC?? I have only been using a high pH antigen retrieval solution and that works fine for me. Thank you for your time, Cathy Cathy M. Mathis Research Lab Technician IV Dept. of Pathology \ Comparative Pathology Lab Wake Forest University School of Medicine p 336.716.1538 cmmathis at wakehealth.edu https://urldefense.com/v3/__http://www.wakehealth.edu__;!!AU3bcTlGKuA!BgMR6haspl_4gdQf833nb8mMORTdWzzfa2esu2-m8htjKWACWx0I_-SRc86hplyehfCBzhg4IViqQ7Ub_00gWjWxfmz0DggTmile4g$ ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. 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From Valerie.Hannen at parrishmed.com Wed Jun 18 10:54:00 2025 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 18 Jun 2025 15:54:00 +0000 Subject: [Histonet] [EXTERNAL] Re: Release of Tissue Policy/ form In-Reply-To: <844138244.1233291.1750119613619@mail.yahoo.com> References: <844138244.1233291.1750119613619@mail.yahoo.com> Message-ID: Richard, Thank you so much!!\ v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From: richard cartun Sent: Monday, June 16, 2025 8:20 PM To: Histonet at lists.utsouthwestern.edu; Hannen, Valerie Subject: [EXTERNAL] Re: [Histonet] Release of Tissue Policy/ form ________________________________ CAUTION: This email originated from outside of Parrish Healthcare. Please do not click on links or open attachments unless you recognize the sender and know the content is safe. If you are unsure or have questions, please contact the IT Department, ext. 6167, or submit an IT ticket. Actual Sender Address: rcartun at sbcglobal.net Begin Original Message: Hi, Valerie! I worked in Pathology at a hospital here in Connecticut for many years and handled all these requests, sometimes in conjunction with our Legal Department. The State of Connecticut has a statute that states human tissue can only be handled by medical personnel in hospitals, surgery centers, labs, or funeral homes. Therefore, we would only release patient specimens that were in formalin (mostly fetuses for burial) to a representative from a funeral home. The patient or family member would have to contact the funeral home for pickup and pay for all associated charges. We also had a release form that the requestor and the funeral home would have to complete and sign off on. With the exception of a fetus for burial, these requirements almost always resulted in patients canceling their initial request. I also found that speaking with the requester and educating them on this issue helped, too. For example, many towns have ordinances that prohibit burying human tissue on the homeowner's property. For patients who wanted their "fresh" placenta after giving birth, our hospital policy was for the OB Department to handle it; Pathology did not get involved! I hope this information helps. Richard W. Cartun, MS, PhD Morphologic Proteomics, LLC On Monday, June 16, 2025 at 03:53:06 PM EDT, Hannen, Valerie via Histonet > wrote: Good Afternoon all!! I am hoping someone can share with me their policy and form that is used in accordance of the policy on the subject of releasing tissue per patient request to the patient? We have had a few incident recently where the patient/ family has asked for placenta and or the fetus ( less than 20 weeks gestation). My concern is that these specimens or any specimen in formalin is the formalin! We have a very old policy and I am not comfortable with it. I would like to use other's policies as a guide (not copy it verbatim). Any suggestions or help would be greatly appreciated. TIA. v/r, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Jun 18 13:35:37 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 18 Jun 2025 14:35:37 -0400 Subject: [Histonet] =?utf-8?q?The_HOT_Histo_Summer_Continues_with_Exciting?= =?utf-8?q?_Leadership_Opportunities_in_Histology_=E2=80=93_Texas?= =?utf-8?q?=2C_Florida=2C_and_California!?= Message-ID: <020701dbe07f$ca9fa490$5fdeedb0$@earthlink.net> Hey there Histonetters! I hope you're having a fantastic day! The HOT Histo Summer is in full swing!! I'm thrilled to share some incredible leadership opportunities that could be the perfect next step for you?or someone you know: ? ? Start fresh and lead your very own brand-new lab in Texas ? ? 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Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net From tkngflght at yahoo.com Mon Jun 23 16:01:55 2025 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 23 Jun 2025 16:01:55 -0500 Subject: [Histonet] =?utf-8?q?Open_position-_Tx_Children=E2=80=99s?= References: <6C0CD010-A3D2-405F-8629-D7EE4D118F8B.ref@yahoo.com> Message-ID: <6C0CD010-A3D2-405F-8629-D7EE4D118F8B@yahoo.com> All - looking for an early AM tech for my bright happy crew at one of the best children?s hospitals in the country: Texas Childrens Hospital. Embed cut stain fs specials grossing and lots of variety across each day. The crew is smart, happy and yes - fun! Being super careful on this hire as I want to retire eventually from this amazing culture of great work and awesome people. Hit me up or apply on Indeed. (The indeed post has a mistake - HT(ASCP) is required, but the HTL is listed). From tkngflght at yahoo.com Mon Jun 23 16:01:55 2025 From: tkngflght at yahoo.com (Cheryl) Date: Mon, 23 Jun 2025 16:01:55 -0500 Subject: [Histonet] =?utf-8?q?Open_position-_Tx_Children=E2=80=99s?= References: <6C0CD010-A3D2-405F-8629-D7EE4D118F8B.ref@yahoo.com> Message-ID: <6C0CD010-A3D2-405F-8629-D7EE4D118F8B@yahoo.com> All - looking for an early AM tech for my bright happy crew at one of the best children?s hospitals in the country: Texas Childrens Hospital. Embed cut stain fs specials grossing and lots of variety across each day. The crew is smart, happy and yes - fun! Being super careful on this hire as I want to retire eventually from this amazing culture of great work and awesome people. Hit me up or apply on Indeed. (The indeed post has a mistake - HT(ASCP) is required, but the HTL is listed). From relia1 at earthlink.net Tue Jun 24 10:59:53 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 24 Jun 2025 11:59:53 -0400 Subject: [Histonet] New Blog post UP! Don't Sleep on you Career This Summer! Plus exciting opportunities! Message-ID: <00c401dbe521$07863900$1692ab00$@earthlink.net> Hi Histopeeps! ?? Summer isn?t just for vacations?it?s prime time for career transformation. Whether you're aiming for a promotion, planning a big pivot, or even considering relocation, this season offers a golden opportunity to make strategic moves while others hit pause. In my latest blog post, I?m sharing why summer is your secret advantage in the job market?and how histology professionals (yes, you #histopeeps!) can leverage the season to shine. >From reduced competition and increased interview flexibility to tons of networking chances and fresh clarity, now is the moment to act. ? Read: ?Don't Sleep on Your Career This Summer?Make It Your Season of Growth!? https://www.linkedin.com/pulse/dont-sleep-your-career-summer-pam-barker-gj3 qf Let?s make the sunshine work for your future. ? I have excellent opportunities in some of the most desirable areas of the U.S.?including TX, VA, CA, FL, TN, and more! ? Contact me for more info: 407-353-5070 ? Email: relia1 at earthlink.net Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?(407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From Johanna.Haddock at propath.com Thu Jun 26 13:42:30 2025 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Thu, 26 Jun 2025 18:42:30 +0000 Subject: [Histonet] Processor - Temp settings Message-ID: Hello, Are you correcting the temperature in processors to match your paraffin product specifications (i.e., melting point of 50-54 ?C)? An external inspector pointed this out. Thanks, Johanna From gu.lang at gmx.at Fri Jun 27 00:31:32 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 27 Jun 2025 07:31:32 +0200 Subject: [Histonet] Processor - Temp settings In-Reply-To: References: Message-ID: <000c01dbe724$bec2adb0$3c480910$@gmx.at> Processor temperature should be set at 2 degrees above melting temperature. With low melting paraffin as you have, it has the advantage of lesser heat-damage to tissue. But I personally think 60?C are ok and you have the advantage of more soluble paraffin for a better infiltration. There is no damage of paraffin at that temperature and in the usual duration of use. Which temperature are you using now? Gudrun -----Urspr?ngliche Nachricht----- Von: Johanna Haddock via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 26. Juni 2025 20:43 An: Histonet Betreff: [Histonet] Processor - Temp settings Hello, Are you correcting the temperature in processors to match your paraffin product specifications (i.e., melting point of 50-54 ?C)? An external inspector pointed this out. Thanks, Johanna _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amrit.Samra at hli.ubc.ca Fri Jun 27 12:00:54 2025 From: Amrit.Samra at hli.ubc.ca (Amrit Samra) Date: Fri, 27 Jun 2025 10:00:54 -0700 Subject: [Histonet] Histonet Digest, Vol 259, Issue 13 In-Reply-To: References: Message-ID: <685ECE460200008D000CE2D2@mail.hli.ubc.ca> I will be away from June26th and back July 2nd.