From jaustin1967 at gmail.com Mon Jul 7 17:45:05 2025 From: jaustin1967 at gmail.com (Michael Bradley) Date: Mon, 7 Jul 2025 18:45:05 -0400 Subject: [Histonet] Clarapath Message-ID: Hello Histonet Does anyone know anything about Clarapath? They have an truely automated microtome called the Sectonstar. Has anyone seen it or demoed it? Thank you From litepath2000 at yahoo.com Tue Jul 8 12:44:43 2025 From: litepath2000 at yahoo.com (LitePath) Date: Tue, 8 Jul 2025 17:44:43 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 260, Issue 1 In-Reply-To: References: Message-ID: <101816606.770915.1751996683315@mail.yahoo.com> i just tiredt add him as friend and submitted as a request,? if you can contact him and ask him to accept, i can go ahead and retrymy venmo profile id is luis chiriboga at luis-chiriboga-4 Luis Chiriboga PhDNSH? DirectorAssistant Professor of Pathology?Associate Professor of Medical Laboratory Science? Please visit the Journal Of Histotechnology Website Please visit the NSH website On Tuesday, July 8, 2025 at 01:00:05 PM EDT, histonet-request at lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. Clarapath (Michael Bradley) Hello Histonet Does anyone know anything about Clarapath?? They have an truely automated microtome called the Sectonstar.? Has anyone seen it or demoed it? Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcokertx at gmail.com Tue Jul 8 12:54:30 2025 From: mcokertx at gmail.com (Michelle Bell) Date: Tue, 8 Jul 2025 17:54:30 +0000 Subject: [Histonet] Clarapath In-Reply-To: References: Message-ID: I have seen the instrument and have talked with them. Great group and very interesting concept. What info are you looking for? ________________________________ From: Michael Bradley via Histonet Sent: Monday, July 7, 2025 5:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Clarapath Hello Histonet Does anyone know anything about Clarapath? They have an truely automated microtome called the Sectonstar. Has anyone seen it or demoed it? Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tcampbell at fgamd.com Wed Jul 9 13:14:11 2025 From: tcampbell at fgamd.com (Tasha Campbell) Date: Wed, 9 Jul 2025 18:14:11 +0000 Subject: [Histonet] Lab Assistant Performance Review Message-ID: Hello Histonetters! Could anyone share the Performance review forms for Lab Assistants? Even histotech would help me. I am not talking about the competencies but the evaluation criteria your lab/hospital uses to base employee raises. Thanks!! Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 Phone: 301-695-6800 ext. 144 Fax: 301-695-6891 From plucas at biopath.org Wed Jul 9 15:16:44 2025 From: plucas at biopath.org (Paula) Date: Wed, 9 Jul 2025 13:16:44 -0700 Subject: [Histonet] VIP Message-ID: <003001dbf10e$654b6e40$2fe24ac0$@biopath.org> Hello, We have two VIP 3000's and we are looking to sell both. One is in working condition, the other one is for parts. If anyone is interested, please contact me. If anyone can suggest whom to contact to sell them to, please let me know. Thank you, Paula Lucas Bio-Path Medical Group 17150 Newhope St #117 Fountain Valley, CA 92708 From gu.lang at gmx.at Thu Jul 10 02:06:20 2025 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 10 Jul 2025 09:06:20 +0200 Subject: [Histonet] Clarapath In-Reply-To: References: Message-ID: <001b01dbf169$2427cd90$6c7768b0$@gmx.at> Hi Michelle, I would like to know, if there are limitations of the autonomous microtome. Hard blocks? Tiny specimens? Underprocessed tissue? Have you got information about this? Thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Michelle Bell via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 8. Juli 2025 19:55 An: Michael Bradley; Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Clarapath I have seen the instrument and have talked with them. Great group and very interesting concept. What info are you looking for? ________________________________ From: Michael Bradley via Histonet Sent: Monday, July 7, 2025 5:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Clarapath Hello Histonet Does anyone know anything about Clarapath? They have an truely automated microtome called the Sectonstar. Has anyone seen it or demoed it? Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at ohiohealth.com Fri Jul 11 10:19:20 2025 From: Kelly.Pairan at ohiohealth.com (Pairan, Kelly) Date: Fri, 11 Jul 2025 15:19:20 +0000 Subject: [Histonet] pH of Roche Reaction Buffer Message-ID: Good Morning, How are you pHing the reaction buffer for the Ventana Benchmark Ultra? Roche doesn't have a published pH for diluted reaction buffer. CAP requirement ANP.22500 states that pH must be tested when a new batch is prepared or received. Are you testing the reaction buffer after you dilute the concentrate (ie: preparing a new batch)? Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: kelly.pairan at ohiohealth.com Work: (614) 566-3575 Cell: (614) 312-0104 From bcooper at chla.usc.edu Fri Jul 11 11:10:29 2025 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 11 Jul 2025 16:10:29 +0000 Subject: [Histonet] pH of Roche Reaction Buffer In-Reply-To: References: Message-ID: Hi Kelly, We measure the pH and document after preparation. We've never had to adjust it; the pH has always fallen within Roche's defined ranges. While the bottle of concentrate itself doesn't mention it, it is printed on the carboy container itself (provided by Roche) and it's in the user manual (page 112 on the version I just looked at). The pH of the diluted wash should be 7.4-7.8. If it falls outside that, contact Roche. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: Pairan, Kelly via Histonet Sent: Friday, July 11, 2025 8:20:06 AM To: 'histonet at lists.utsouthwestern.edu' Subject: [Histonet] pH of Roche Reaction Buffer (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good Morning, How are you pHing the reaction buffer for the Ventana Benchmark Ultra? Roche doesn't have a published pH for diluted reaction buffer. CAP requirement ANP.22500 states that pH must be tested when a new batch is prepared or received. Are you testing the reaction buffer after you dilute the concentrate (ie: preparing a new batch)? Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: kelly.pairan at ohiohealth.com Work: (614) 566-3575 Cell: (614) 312-0104 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1MyA-b1C39589MhhwQL6JkBVVoO2NiPCh_bTw2ge74axqJgmVhSC1NJo0pULgXbc9dTX5XY-URwEdWQOrwcNINcglOY5B4Ec6ouCTPmNSA9Glz1Qp-Pwe18bAGtIRSFF6sUSlDUJPLLfLmBeUDGmnv2guRbUQHdEtdVguiXeSEXuO4E2yHeWpM_oeHHbQqEUgUhAuZUG_8G0FPZoDTF64lX5U5Db3uk9iR7ovOnP3REPFmCQlfsIiX1GT3dOhCL75XGZm7ZucKsCiKTyVepd_vclHreYt2f5a2zi_4DSQ6pFbAdf8L5lGQjJigaWfuGVdG7uKGMKUR9kCOf00dihXEQK9Gqdc0FVnfJ_zUChHrGhA1yhtQb-LIGinhYotFwi755tYwBDWZM_wG_NiKTogMRGWf7AjjkUH8ghqjuTVyih-Z7Ai0yFcvjd2vwGqseDn/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From bcooper at chla.usc.edu Fri Jul 11 17:01:52 2025 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 11 Jul 2025 22:01:52 +0000 Subject: [Histonet] Kappa and Lambda ISH on Leica Bond assistance Message-ID: Happy Friday Histonet! For those of you doing Kappa and Lambda by ISH on the Leica Bond, can you please tell me specially which hybridization protocol, and enzyme concentration/timing you're using? Any tweaks? As these probes are ASR, the vendor can't really offer any technical advice on this matter. But YOU can. Talk to me Histonet! Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From mcokertx at gmail.com Fri Jul 11 17:14:48 2025 From: mcokertx at gmail.com (Michelle Bell) Date: Fri, 11 Jul 2025 22:14:48 +0000 Subject: [Histonet] Clarapath In-Reply-To: <001b01dbf169$2427cd90$6c7768b0$@gmx.at> References: <001b01dbf169$2427cd90$6c7768b0$@gmx.at> Message-ID: Hi Gudrun, It?s a really interesting concept. The demonstration I was able to watch showed large and small specimens. As with any new technology, I am sure their entire process is very controlled (reagents controlled, processing controlled, etc). I do know that they?re using a technology that involves tape over the water method, which I believe most automated microtomes are using at this point. Clarapath presented at the Executive War College this year, which is where I witnessed their demonstration. It?s really fascinating because the operator can work remotely. The only limitation I would see to this is of a malfunction occurs. I found the unit fascinating, like a glimpse into the future. To answer your questions I would say this: Automation requires a tradeoff. A technician can overcome poor quality while a robot may be less capable of this, however, when we have under processed tissue that explodes on a waterbath, that won?t happen with this tape method. The technician still has ALOT of control with their units, and this means microtomy of hard or tiny specimens can be adjusted. All of the automated microtomes promise this. A demo would be the only way to be sure, though. Have a great weekend! M ________________________________ From: Gudrun Lang Sent: Thursday, July 10, 2025 2:06 AM To: 'Michelle Bell' Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Clarapath Hi Michelle, I would like to know, if there are limitations of the autonomous microtome. Hard blocks? Tiny specimens? Underprocessed tissue? Have you got information about this? Thanks Gudrun -----Urspr?ngliche Nachricht----- Von: Michelle Bell via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 8. Juli 2025 19:55 An: Michael Bradley; Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Clarapath I have seen the instrument and have talked with them. Great group and very interesting concept. What info are you looking for? ________________________________ From: Michael Bradley via Histonet Sent: Monday, July 7, 2025 5:58 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Clarapath Hello Histonet Does anyone know anything about Clarapath? They have an truely automated microtome called the Sectonstar. Has anyone seen it or demoed it? Thank you _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet