From Jessica.Piche at wtbyhosp.org Thu Apr 3 11:11:00 2025 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 3 Apr 2025 16:11:00 +0000 Subject: [Histonet] ambient room temperature in autopsy suite Message-ID: Good Afternoon Everyone, Does anyone know if there is a regulatory temperature and humidity level for the autopsy suite? I am looking for temperature and humidity ranges. Thank you, Jessica Jessica Pich?, HT(ASCP) Histology Team Leader, Laboratory Waterbury Health 64 Robbins Street Waterbury, Connecticut, 06708 Phone: 203-573-7167 FAX: 203-573-7242 Email:jessica.piche at wtbyhosp.org Waterbury HEALTH From SThompson4 at sonichealthcareusa.com Thu Apr 3 12:04:25 2025 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Thu, 3 Apr 2025 17:04:25 +0000 Subject: [Histonet] Hiring Histology Technician/Technologist - Sonic Healthcare USA - sign-on bonus - relocation assistance Message-ID: <6c26ae65dfca4aaf88785ba66d3610e4@sonichealthcareusa.com> Are you a night owl? Live in beautiful Fall River, MA working at our New England Tissue Issue Lab. Sonic Healthcare USA has been certified as one of the Great Places to Work! Jan 2025 - Jan 2026 LOCATION: 1822 North Main Street, Fall River, MA 02720 HOURS: shift start between 8:00pm-10:00pm; Monday - Friday, (8hrs) FULL TIME: Benefits Eligible **eligible for shift differential** In this role, you will: * Perform a vital part of the patient care process through embedding and preparing slides for routine H & E and special stain procedures * Performs and documents scheduled preventative maintenance * Recognize when troubleshooting is needed for processing, embedding, cutting, staining All you need is: * Associates Degree in Biology or other related science field; Bachelor's of Science in Biology or related science field preferred. * ASCP HT/HTL certified or eligibility to sit for the certification exam required; and must meet State and Federal requirements for the position and duties to be performed. * Previously worked in the Histology field for 1 year (preferred) * At least 1 year of laboratory experience (preferred) Please contact: Stephanie Thompson, sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From Allison.Spriggs-Scott at harrishealth.org Thu Apr 3 16:54:09 2025 From: Allison.Spriggs-Scott at harrishealth.org (Spriggs-Scott, Allison D) Date: Thu, 3 Apr 2025 21:54:09 +0000 Subject: [Histonet] Fw: Thyroid with tumor for BRAF In-Reply-To: References: , Message-ID: ________________________________ From: Spriggs-Scott, Allison D Sent: Thursday, April 3, 2025 4:35 PM To: Histonet at lists.utsouthwestern.edu Subject: Fw: Thyroid with tumor for BRAF ________________________________ From: Spriggs-Scott, Allison D Sent: Thursday, April 3, 2025 3:45 PM To: histonet at lists.utsouthwestern.edu Subject: Thyroid with tumor for BRAF Hello to all in histoland. I am in need of thyroid with tumor for BRAF. Its hard to come by. Any help will be greatly appreciated. Allison Scott HT(ASCP) LBJ Hospital Houston Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From durmtissuebank01 at gmail.com Thu Apr 10 19:18:09 2025 From: durmtissuebank01 at gmail.com (Ni Nie) Date: Thu, 10 Apr 2025 20:18:09 -0400 Subject: [Histonet] Control Slides Message-ID: Can anyone give me their opinion on control slides, both special stains and IHC? Who provides the best quality and gives the best support? Who has them in stock when you need them? We are inquiring about human tissue, not animal or synthetic. Dr. Su Suyuan Durham Tissue Bank From relia1 at earthlink.net Fri Apr 11 11:25:55 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 11 Apr 2025 12:25:55 -0400 Subject: [Histonet] Short on staff? Here's your chance to make a difference AND earn a referral bonus! Message-ID: <000001dbaafe$6790f590$36b2e0b0$@earthlink.net> Hey Histopeeps! Looking for help in your lab? Short on staff? Here's your chance to make a difference AND earn a referral bonus! If you share with me the contact info of your supervisor, manager, or director, and I secure the job and place someone in the role... You'll earn a referral fee-just like referring a candidate! You can choose: * To let your supervisor know you're referring me, OR * Keep it confidential-whatever works for you. It's simple! Just email me at relia1 at earthlink.net with their name and email, and I'll handle the rest. Let's work together to help your team thrive! Have an amazing day, histopeeps! p.s IT's Friday! Yay! Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! From carl.hobbs at kcl.ac.uk Sat Apr 12 12:26:18 2025 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 12 Apr 2025 17:26:18 +0000 Subject: [Histonet] Histology site to upload images to Message-ID: Hi I used to upload my IHC/IF/stains images to Histonet Image archive.? I'm kindly told that it has been taken down....with all my images gone Is there another site that is well-subscribed, that HistoJunkies like me can upload images to? An image archive is a great reference eg: when I get a totally new primary ab ( that I haven't used before/don't know the protein expression pattern) I will use the most excellent HPA to find out info re that protein/expression pattern Unfortunately it is human-specific ( but, if they have images and give their abs a green circle rating, I can trust the expression pattern/choose the most appropriate tissue to test my new abs) I work/have worked? with FS and Pwax sections of mouse/rat/fly, axolotl/hu/chick/Zfish/snake for eg. Thank you for getting here Be most grateful for suggestions Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge? Kings College London London SE1 1UL ? 020 7848 6810 From durmtissuebank01 at gmail.com Mon Apr 14 10:03:10 2025 From: durmtissuebank01 at gmail.com (Ni Nie) Date: Mon, 14 Apr 2025 11:03:10 -0400 Subject: [Histonet] Copper Staining Message-ID: Does anyone have any tricks for Copper staining? Our techs always struggle with it. Sometimes it looks good right after staining, but it fades after a few days. Other times after lengthy staining we just don't get good results. From tbraud at redeemerhealth.org Mon Apr 14 13:02:29 2025 From: tbraud at redeemerhealth.org (Terri Braud) Date: Mon, 14 Apr 2025 18:02:29 +0000 Subject: [Histonet] Copper Stain Message-ID: <9242080c70aa4c55b59baac2c9db5d07@redeemerhealth.org> Always use Ethanol (the drinking kind) not reagent alcohol. It makes a huge difference Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Monday, April 14, 2025 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 257, Issue 6 CAUTION: This is an external email. Stop and think before clicking links or opening attachments. If you have questions or suspicions, call IT Support Center 215-938-3900 or ext. 4HELP. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Copper Staining (Ni Nie) ---------------------------------------------------------------------- Message: 1 Date: Mon, 14 Apr 2025 11:03:10 -0400 From: Ni Nie To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Copper Staining Message-ID: Content-Type: text/plain; charset="UTF-8" Does anyone have any tricks for Copper staining? Our techs always struggle with it. Sometimes it looks good right after staining, but it fades after a few days. Other times after lengthy staining we just don't get good results. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 257, Issue 6 **************************************** ***Please Note: Redeemer Health has now changed its email domain from @holyredeemer . com to @redeemerhealth . org. Please alert your IT/cybersecurity team to ensure our new email domain is safe-listed.*** This email, and any files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. If you are not the intended recipient, please telephone or email the sender and delete this message and any attachment from your system; you must not copy or disclose the contents of this message or any attachment to any other person. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Redeemer Health. Redeemer Health may monitor email traffic data. From afhenwood at outlook.com Mon Apr 14 16:13:01 2025 From: afhenwood at outlook.com (Tony Henwood) Date: Mon, 14 Apr 2025 21:13:01 +0000 Subject: [Histonet] Copper Staining In-Reply-To: References: Message-ID: We have found issues with rhodanine staining: A common problem with this stain is the gradual fading of rhodanine staining over several days, particularly in biopsy specimens from patients with Primary Biliary Cirrhosis and when copper is scanty (Jain et al 1978 Journal of clinical pathology, 31(8), 784-790). A similar problem was noted by Lindquist (1969, Archives of pathology, 87(4), 370-379), using Permount as a mounting medium for the sections. Jain et al (1978 Journal of clinical pathology, 31(8), 784-790) found that the degree of fading differed according to the mounting medium used. This was greatest with Eukitt, less with DPX and Diatex, and least with Ralmount (all products of Raymond A. Lamb). We found that aqueous mountants such as Aquamount preserved staining. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Ni Nie via Histonet Sent: 15 April 2025 01:03 To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Copper Staining Does anyone have any tricks for Copper staining? Our techs always struggle with it. Sometimes it looks good right after staining, but it fades after a few days. Other times after lengthy staining we just don't get good results. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Apr 15 10:29:34 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 15 Apr 2025 11:29:34 -0400 Subject: [Histonet] New Blog Post UP! Unlock Your Workplace Superpower: Why Self-Awareness and Soft Skills Are Your Secret Weapon Message-ID: <012801dbae1b$317e9190$947bb4b0$@earthlink.net> Hi Histonetters! I have a New Blog Post UP! Unlock Your Workplace Superpower: Why Self-Awareness and Soft Skills Are Your Secret Weapon https://www.linkedin.com/pulse/unlock-your-workplace-superpower-why-self-aw areness-pam-barker-2xwwf Also, if you are looking for an amazing opportunity or a fantastic histotech I am just a text, email or phone call away. Have a great day! #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologylife #histologyjobs #histologycareers ???? Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: ? ? ? ? ? (407)353-5070 FAX: ? ? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! From melissa at alliedsearchpartners.com Tue Apr 15 12:03:50 2025 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Tue, 15 Apr 2025 17:03:50 +0000 Subject: [Histonet] Per Diem Histotechs Needed in Urbana, IL Message-ID: Hello- I am needing some Temporary/Fill In Histotechs in and around Urbana, IL to help with coverages in a lab starting ASAP. ASCP not required/just preferred. Must be proficient cutter and embedder for dermatology. The hours needed are 5:30a or 6:30a start time. If you can help fill in and provide coverages in this area of the country please do not hesitate to reach out. Melissa Owens Allied Search Partners melissa at alliedsearchpartners.com 386-339-0839 From szigeti.csaba at med.u-szeged.hu Wed Apr 16 03:05:47 2025 From: szigeti.csaba at med.u-szeged.hu (Dr. Szigeti Csaba) Date: Wed, 16 Apr 2025 10:05:47 +0200 Subject: [Histonet] Fluorescent in situ hybridization Message-ID: <2a4f9f4bc91e225b2e5e67e4074a6dd4@med.u-szeged.hu> Dear Histonetters! I should optimise Fluorescent labneled DNA oligo probe in FISH I would like to enhance the specificity of the signal, My protocol: 2*5 min 2xcSSC wash prehibridization, 0.75% triton, than ON hybridization at 30 Grad Celsius in puffer (FISH oligoprobe 25 bp DNA fluorescent labeled) Post hibr wash: 30 min. 10%formamide in 2x ssc 30 Grad celsius+nissl counterstain. Than rinse in 2x ssc and cover. Result: sometimes weak signal, but very much aspecific dye particles. Would you have some idea in mind to help me in this protocol. E.g: FA fixation at the start is crucial, what you think? Many thanks in advance to all of you! Best regards Csaba Szigeti -- DR. SZIGETI CSABA MSC. PHD. EGYETEMI ADJUNKTUS Szegedi Tudom?nyegyetem ?ltal?nos Orvostudom?nyi Kar Anat?miai Sz?vet-?s Fejl?d?stani Int?zet From relia1 at earthlink.net Wed Apr 16 10:52:16 2025 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 16 Apr 2025 11:52:16 -0400 Subject: [Histonet] RELIA HOT JOB ALERT!! Exciting Opportunities in Nashville, TN!! Message-ID: <002f01dbaee7$98e84980$cab8dc80$@earthlink.net> Calling all Histopeeps! Nashville is on fire?hotter than Nashville chicken! My client a cutting-edge lab in Nashville is in GROWTH MODE, and we?re hiring for full-time, permanent DAY shift positions. This is your chance to step into a role that offers: Top-notch compensation Generous shift differentials Fantastic benefits Relocation and sign-on bonuses And above all, incredible growth opportunities! Join one of the most STELLAR labs in Nashville, where you?ll live and work in one of the most vibrant, exciting cities in the country! Don?t miss this opportunity to elevate your career. Let?s talk! text/call at 407-353-5070, or email me at Relia1 at Earthlink.Net. Thanks-Pam "The secret of getting ahead is getting started." - Mark Twain. Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ???? From jkiernan at uwo.ca Wed Apr 16 23:33:19 2025 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 17 Apr 2025 04:33:19 +0000 Subject: [Histonet] Churukian's Manual PDF Message-ID: Sadly, the last version (2009) of the late Chuck Churukian's Manual of the Special Stains Laboratory is now no longer available from the University of Rochester's Pathology website. Does anyone have a copy of the complete PDF file? The Biological Stain Commission would like to make this available to everyone. If you have a copy of the complete PDF, please contact me (jkiernan at uwo.ca) to arrange how to do an upload. John Kiernan Trustee, Biological Stain Commission, Inc. = = = From dclunie at dclunie.com Thu Apr 17 09:29:45 2025 From: dclunie at dclunie.com (=?UTF-8?Q?David_Clunie?=) Date: Thu, 17 Apr 2025 14:29:45 +0000 Subject: [Histonet] Churukian's Manual PDF In-Reply-To: References: <7cd9d9a9-69b3-4a69-8db3-0e7f942945d0@dclunie.com> Message-ID: <010001964427df66-f62adab8-f853-4177-bad5-a9d24464545d-000000@email.amazonses.com> Hi John Are you referring to this: https://www.urmc.rochester.edu/MediaLibraries/URMCMedia/urmc-labs/pathology/about-us/images/Method-of-Histochemical-Stains-Diagnostic-Application.pdf It is also archived: https://web.archive.org/web/20240710002832/https://www.urmc.rochester.edu/MediaLibraries/URMCMedia/urmc-labs/pathology/about-us/images/Method-of-Histochemical-Stains-Diagnostic-Application.pdf David On 4/17/25 12:33 AM, John Kiernan via Histonet wrote: > Sadly, the last version (2009) of the late Chuck Churukian's Manual of the Special Stains Laboratory is now no longer available from the University of Rochester's Pathology website. > > Does anyone have a copy of the complete PDF file? The Biological Stain Commission would like to make this available to everyone. If you have a copy of the complete PDF, please contact me (jkiernan at uwo.ca) to arrange how to do an upload. > > John Kiernan > Trustee, Biological Stain Commission, Inc. > = = = > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sat Apr 19 12:53:33 2025 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 19 Apr 2025 17:53:33 +0000 Subject: [Histonet] Histology images Message-ID: Hi I used to upload my Histology images to Histonet Images archive I have been informed that this no longer exists: hence my images are "LOST" I'd be grateful if somebody would suggest where best to upload my images, to share? It's good to share-ily Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge? Kings College London London From john at imebinc.com Mon Apr 28 13:50:40 2025 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Mon, 28 Apr 2025 11:50:40 -0700 Subject: [Histonet] Removing H&E coverslippped slide Message-ID: Histonetters Can you experts in staining and Coverslipping offer the number of times or % of slides recover slipped to a differ stain, any opinion is appreciated John IMEB Inc