From rcartun at sbcglobal.net Mon Sep 2 20:15:06 2024 From: rcartun at sbcglobal.net (richard cartun) Date: Tue, 3 Sep 2024 01:15:06 +0000 (UTC) Subject: [Histonet] Antibody dilution help In-Reply-To: References: Message-ID: <231661194.2881228.1725326106690@mail.yahoo.com> Hi, Charles: You may already have your answer by now, but I wanted to comment on preparing antibody dilutions. Whenever I taught someone (or a group) how to prepare antibody dilutions, I emphasized two things: 1.) simplicity and 2.) consistency.? I have found over the years that many people struggle with dilution calculations because mathematics is involved, and, believe me, we all experience "Brain Fog" at one time or another when doing this.? I have always used a simple equation to calculate dilutions: Dilution Factor = Total Volume (Diluent + Antibody) / Volume of Antibody????????????*** Make sure to use "mL" units for your calculations. To solve this equation you must know two of the three variables ...... correct?? In your situation, the Dilution Factor is "100", and the Volume of the Antibody is "100 microliters" or 0.1 mL? If you multiply the Dilution Factor (100) by the Volume of the Antibody (0.1 mL) you get "10 mL" for the Volume of the Diluent. Paula brought up a good point ...... some people will subtract the Volume of Antibody from the Total Volume (e.g., 99 parts of diluent and 1 part of antibody).? I never do this (opening the door for error) unless the volume of the antibody is equal to or greater than 100 microliters (0.1 mL).? Therefore, in your situation, I would add the 0.1 mL of antibody to 9.9 mL of diluent to yield 10 mL of a 1:100 dilution. Let me ask you a question ....... Do you need 10 mL of diluted antibody?? If so, great, but let's say you only required 1.0 mL of diluted antibody, how would you solve the equation? Dilution Factor (100) = Total Volume (1.0 mL) / "X" mL of Antibody If you solve for "X" you would get 0.01 mL or 10 microliters.? Does that make sense? I only used simple dilutions in my laboratory.? For example, if I need at least 1 mL of antibody: 1:5?? (0.8 mL diluent + 0.2 mL antibody) 1:10?? (0.9 mL diluent + 0.1 mL antibody) 1:20?? (1.0 mL diluent + 0.05 mL antibody 1:50?? (1.0 mL diluent + 0.02 mL antibody) 1:100 ? (1.0 mL diluent + 0.01 mL antibody) ? 1:200?? (1.0 mL diluent + 0.005 mL antibody) 1:500?? (2.5 mL diluent + 0.005 mL antibody) 1:1,000?? (5.0 mL diluent + 0.005 mL antibody) ...... and we never encountered any problems.? If you have multiple people making dilutions in your laboratory, everyone has to do it the same way ...... be consistent!? Remember, the number of slides that you have to test should determine how much antibody you need and the pipettes available to you will determine what volumes you can pipette. I hope this was helpful. Richard W. Cartun, PhD, MSMorphologic Proteomics, LLC(860) 490-7633 Cell On Friday, August 30, 2024 at 10:57:51 AM EDT, Charles Riley via Histonet wrote: Hello everyone, Not sure if it's just burnout or Covid brain but I am having issues with remembering the right way to dilute my antibodies, and all the calculators online seem to give different responses. I have 100 microliters of 1mg/mL antibody and I want to make a 1:100 dilution. How much diluent do I add to do this?? And if anyone has any calculators that they like using that would be awesome too _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Sep 4 12:32:24 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 4 Sep 2024 13:32:24 -0400 Subject: [Histonet] What Did YOU do on Your Summer Vacation and Do You Want to Go BACK? Message-ID: <001301dafef0$686274e0$39275ea0$@earthlink.net> Hi Histopeeps! ? How was your summer vacation? ??? Did you visit any place you?d love to move to? ? Whatever your dream destination, I can help make it a reality! ? There?s still time this year to make that move, or we can plan for fall ? or even 2025. ? I have opportunities in labs across the country, offering drama-free, permanent full-time positions with excellent compensation ?, benefits, and relocation/sign-on bonuses! ? Current openings: * ? Histology Manager * ? Histology Supervisor * ? Lead Histology Tech * ? Histotechnician * ? Histotechnologist Exciting locations: * ? TX: Lead a successful lab! * ? AZ: Lead Tech/Supervisor role * ?? VA: Up to $20K sign-on bonus! * ? FL: I?ll help with your FL license! * ? WA: Great growth opportunity! * ? TN: Histotech & Supervisor roles in Nashville! * ? AZ: Mohs Tech with an amazing doctor! * ? WI: Unique leadership role New opportunities are coming in every day! ? 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If you?ve ever considered moving to Nashville, this is the lab you want to be in! Why You?ll Love It: Work in a BRAND-NEW LAB with WINDOWS! ? Perform routine histology and staining ? Dive into immunohistochemistry ? Grow and learn with cutting-edge techniques and equipment onsite ? What My Client Offers: VERY competitive pay rates and shift differentials ? Platinum benefits ? Relocation assistance ? $10K sign-on bonus! ? Ready to make the move? Let?s chat! Don?t miss out on this fantastic opportunity! For more information Or To explore similar opportunities in different locations, let me know! ? Cell/Text: 407-353-5070 email: relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Mon Sep 9 11:28:46 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 9 Sep 2024 12:28:46 -0400 Subject: [Histonet] ALL of THIS And a Bag of Chips Histopeeps!!! All The Things!! Message-ID: <000001db02d5$588e1e00$09aa5a00$@earthlink.net> ? All of this AND a Bag of Chips! ? That?s what you get when you work with RELIA! ? All the things Histopeeps! ALL THE THINGS! ?? ? Exclusive Opportunities ? Access to Hard-to-Find Positions ? A Great Work Environment ? Fantastic Compensation ? Relocation and Sign-On Bonuses ? A Dedicated Consultant to Support You Throughout the Process Not Quite Ready To Make A Move? ? No Worries! Instead Of An Active Job Search, I Can: ? Tune up your resume ? Help with salary information and negotiation ? ?Coach you through career strategies and situations ?Whatever YOU need, Histopeeps, I am here to help! ???? The BEST part? ALL of this is FREE to you! ? These are the services that RELIA provides as a courtesy to the Histology Profession. ? All you have to do is: ? Send me an email at relia1 at earthlink.net to subscribe to my histology careers bulletin. OR ? Join my group on Facebook: www.facebook.com/groups/histo technologists And you can always reach me on my cell/text at 407-353-5070 ? Let?s make your next career move the best one yet! ? #MondayMotivation #JoinUs #ILoveMyHistopeeps #HistologyIsCool #HistologyLab #Histology #Jobs4MyHistopeeps #HistologyJobs #HistologyCareers ?????? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From chak_bou at yahoo.com Mon Sep 9 11:34:48 2024 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Mon, 9 Sep 2024 16:34:48 +0000 (UTC) Subject: [Histonet] Protocol on cutting control slides References: <1214068405.3000267.1725899688264.ref@mail.yahoo.com> Message-ID: <1214068405.3000267.1725899688264@mail.yahoo.com> everyone, I?m reaching out to see if anyone has a protocol for cutting control slides. We need to document all positive controls used in the IHC lab, along with information on who sectioned the slides and when. I?m considering creating a log sheet for the techs to record the date, microtomy details, and their initials. Any input or existing templates would be greatly appreciated. Thanks in advance! Best regards, Sent from Yahoo Mail for iPhone From relia1 at earthlink.net Tue Sep 10 11:46:52 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 10 Sep 2024 12:46:52 -0400 Subject: [Histonet] It's Team Tuesday Histopeeps! Message-ID: <01c601db03a1$0a81d360$1f857a20$@earthlink.net> Happy Team Tuesday, Histopeeps! ? A great team is the BEST thing to HAVE!! Am I right? ? ICYMI, check out my blog post: Building A Cohesive and Happy Histo Team: 5 Essential Steps! ?? ? Read it here! https://reliasolutionspambarker.wordpress.com/2024/07/09/building-a-cohesive -and-happy-histo-team-5-essential-steps/ I?d love to hear your thoughts on my post and, even more importantly, share your tips for creating a terrific team! ?? Is your team not so terrific? ? Thinking about making a change now or in the future? Let me know ? I can HELP! ? ? Direct Message me on social media ? Call/Text: 407-353-5070 ? Email: Relia1 at Earthlink.Net #Tuesday #TeamTuesday #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? Let?s build amazing teams together! ? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! From relia1 at earthlink.net Wed Sep 11 11:25:01 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 11 Sep 2024 12:25:01 -0400 Subject: [Histonet] =?utf-8?q?=F0=9F=8D=8E_Back_to_School=2C_Back_to_Work!?= =?utf-8?q?_=F0=9F=8D=8E_Experience_that_Back_to_School_Vibe_With_a?= =?utf-8?q?n_Exciting_New_JOB_=29?= Message-ID: <005c01db0467$2a243480$7e6c9d80$@earthlink.net> Hey Histopeeps! ? Remember the thrill of new shoes, fresh clothes, and making new friends? 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Thrive in the heart of the Sonoran Desert! ?Mohs and MORE! Don?t wait! Make the right move at the right time. Contact us today to learn more about our opportunities and how we can help you tune your resume for success. ?? For more information Contact me: Direct Msg me on Social Media Cell/Text: 407-353-5070 email: relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Thu Sep 12 11:08:55 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 12 Sep 2024 12:08:55 -0400 Subject: [Histonet] =?utf-8?q?Spilling_the_TEA_on_a_BRAND_NEW_LAB_NEAR_Bos?= =?utf-8?b?dG9uLCBNQSEg8J+nquKcqA==?= Message-ID: <000e01db052e$12b8d860$382a8920$@earthlink.net> Hey Histopeeps! Happy Thursday! ? I?m excited to spill the tea on a RELIA EXCLUSIVE near Boston in a gorgeous suburb! 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Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From relia1 at earthlink.net Fri Sep 13 11:04:44 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 13 Sep 2024 12:04:44 -0400 Subject: [Histonet] Hey Histopeeps! Maybe You want to "Give it a Think" Message-ID: <018201db05f6$a773a7f0$f65af7d0$@earthlink.net> ?Hey Histopeeps! ? Have You Thought About This? * ? Brand New Lab? * ? Better Hours? * ? More Variety in Tasks? * ??? An Amazing Manager to Lead YOU? * ? A Better Team to Work With? * ? Opportunity to LEAD? * ? More Money? * ? A New Location? Take a moment this weekend to think about it. Could any of these make your life better? I can help make them happen! I Have Clients Nationwide Looking For Talented Histopeeps Like YOU! They Offer Some Or ALL Of These Benefits: * ? Brand New Labs! * ? Better Hours! * ? More Variety in Tasks! * ??? An Amazing Manager to Lead YOU! * ? A Better Team to Work With! * ? Opportunity to LEAD! * ? More Money! * ? A New Location! I have NEW positions coming in daily from all over the U.S. Some of my hottest opportunities are located in Massachusetts, Florida, Tennessee, and Arizona. All opportunities are full-time and permanent, offering excellent compensation, including great benefits and pay, plus sign-on/relocation bonuses. Ready to take the next step? Contact me: * ? Direct Message me on Social Media * ? Cell/Text: 407-353-5070 * ? Email: relia1 at earthlink.net #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! From relia1 at earthlink.net Mon Sep 16 12:25:53 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 16 Sep 2024 13:25:53 -0400 Subject: [Histonet] Histopeeps!! What's YOUR #MondayMove? Message-ID: <035801db085d$7ce82150$76b863f0$@earthlink.net> Hey Histopeeps! ? Happy Monday! What?s YOUR #MondayMove? Let?s set the tone for a fantastic week together! Here are some ideas to get you started: * Get Ready for NOLA! ? * Not going to the NSH/SC in NOLA? * No worries! How about: * Signing up for some online classes? ? * Looking into your state society and what they are doing? ?? * Starting a great book? ? * Giving your resume a tune-up? ? * Exploring new opportunities? ? * Having a chat with me about what?s happening in Histo? ? I?m here to help you make that #MondayMove and get you well on your way to a fantastic week. Let?s connect and make it happen! Why? Because? #Ilovemyhistopeeps and #histologyiscool For more information, contact me: * Direct Message me on Social Media * Cell/Text: 407-353-5070 * Email: relia1 at earthlink.net Have a GREAT day and a FANTASTIC week! ? #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? Thanks-Pam ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ?????? From relia1 at earthlink.net Tue Sep 17 10:59:41 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 17 Sep 2024 11:59:41 -0400 Subject: [Histonet] SuperStar Opportunities for SuperStar Histotechs!! Message-ID: <007f01db091a$9d8d6290$d8a827b0$@earthlink.net> Hi Histonetters! I?m thrilled to share some amazing opportunities tailored just for Superstar Histotechs like you! ? What makes you a Superstar? It?s all about finding the perfect match between you and your lab, allowing you to shine and excel in your role. My goal is to help you highlight your experience, talents, and skills to secure that Superstar Opportunity! ? Why these opportunities are special: * Career Growth & Learning: Each position offers the chance to advance and develop your skills. ? * Full-Time & Permanent: Enjoy stability with excellent compensation, great benefits, and sign-on/relocation bonuses. ? Ready to take the next step? Here are some of the most dazzling opportunities available: * MA ? Boston Suburbs: BRAND NEW LAB! DAY Shift! ?? * FL ? Ft. Lauderdale: Day Shift no weekends enjoy South Florida! ? * FL ? North Central Florida: 20% shift diff AND GREAT Benefits. ? * TN ? Nashville: Excellent Compensation and WINDOWS!! ? * AZ ? Tucson: Mohs Tech: Great Doc!!! ?? * AZ ? Sierra Vista: Supervisor/Team Lead ? Opportunity to Grow! ? Histonetters! If you are interested in any of these opportunities, Please Contact Me. I Have SO Much More Information To Share About Them! If these locations aren?t quite right for you, no worries! I have opportunities nationwide, with new ones coming in every day. Whether you?re considering a local job change or relocating; Now or in 2025, it?s never too early to prepare for a Superstar Job! ? Contact me today: ? Direct Message me on Social Media ? Cell/Text: 407-353-5070 ? Email: relia1 at earthlink.net Looking forward to helping you find your perfect fit! Best regards, Pam ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ????? From relia1 at earthlink.net Tue Sep 17 11:43:31 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 17 Sep 2024 12:43:31 -0400 Subject: [Histonet] New Blog Post UP! Unleash YOUR Convention Potential. A Practical Guide to Success! Message-ID: <008d01db0920$bbbe0e80$333a2b80$@earthlink.net> Hey Histopeeps!! Happy Tuesday! Here?s my tip for hashtag#TipTuesday ! I have new blog post up! Going to NOLA for the hashtag#NSH /SC Going to a State Meeting? Going to a Comicon? Whatever type of Gathering You Are Planning on Attending Here is my blog post: Unleash Your Convention Potential: Practical Advice for Success! Hey Histopeeps!! Happy Tuesday! Here?s my tip for hashtag#TipTuesday ! I have new blog post up! Going to NOLA for the #NSHSC Going to a State Meeting? Going to a Comicon? Whatever type of Gathering You Are Planning on Attending Here is my blog post: Unleash Your Convention Potential: Practical Advice for Success! Unleash Your Convention Potential: Practical Advice for Success ? Histopeep Huddle ? Reader ? WordPress.com Have a Great Day Histopeeps!! ?????? Here is the link again to copy and paste: https://reliasolutionspambarker.wordpress.com/2024/09/16/unleash-your-conve ntion-potential-practical-advice-for-success/ Thanks-Pam ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ?????? From relia1 at earthlink.net Wed Sep 18 10:21:25 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 18 Sep 2024 11:21:25 -0400 Subject: [Histonet] Exciting New Opportunties in Massachusetts, Tennessee and Florida! Brand NEW LABS and MORE!! Message-ID: <00d301db09de$6ddef6c0$499ce440$@earthlink.net> Hey There Histopeeps! ? ? Massachusetts, Tennessee, Florida! * ? BRAND NEW LAB! * ? Day Shift OR 20% Shift Diff * ? Relo/Sign On Bonuses * ? Full-time and Permanent M-F * ? Opportunity to Learn, Advance, and Grow! My clients in MA, TN, and FL have all these amazing opportunities in common! Just like these states share some fascinating similarities too! ? ? Discover the Common Threads Between Massachusetts, Tennessee, and Florida! ? ?? Rich History: From the American Revolution in Massachusetts to the Civil War in Tennessee and early Spanish settlements in Florida, history buffs have plenty to explore! ? Natural Beauty: Whether it?s the stunning Cape Cod, the majestic Great Smoky Mountains, or the beautiful beaches of Florida, nature lovers are in for a treat. ? Musical Heritage: Massachusetts? classical and folk tunes, Tennessee?s country music heart in Nashville, and Florida?s vibrant Latin, jazz, and hip-hop scenes make for a diverse musical journey. ? Top Universities: Home to prestigious institutions like Harvard, Vanderbilt, and the University of Florida, these states are educational powerhouses. ? Sports Passion: From the Boston Red Sox and New England Patriots to the Tennessee Titans and Miami Dolphins, sports fans have plenty to cheer for! ? Diverse Economies: With strong sectors in healthcare, education, tourism, and more, these states are economic hubs. ? Ready to take the next step in your histology career? ? ?Contact me today! ? Direct Message me on Social Media ? Cell/Text: 407-353-5070 ? Email: relia1 at earthlink.net #FloridaHistologyJobs #MassachusettsHistologyJobs #TennesseeHistologyJobs #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? From nmargaryan88 at gmail.com Sat Sep 21 18:24:44 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Sat, 21 Sep 2024 18:24:44 -0500 Subject: [Histonet] Animal tissue processing time Message-ID: Hello, Could you please share your processing programs to run mouse, rat, rabbit, dog?s organs after full necropsy? Thank you in advance for sharing your different programs, Naira From portera at msu.edu Sun Sep 22 07:33:12 2024 From: portera at msu.edu (Porter, Amy) Date: Sun, 22 Sep 2024 12:33:12 +0000 Subject: [Histonet] Animal tissue processing time In-Reply-To: References: Message-ID: <4BA8D4DE-BB1B-4EE8-B79E-5B728963E638@msu.edu> I don?t have any information with me at home but would recommend you try to pick up a copy of https://theblock.nsh.org/theblock/discussion/vir-animal-tissue-processing-manual?_gl=1*14211cf*_gcl_au*MjA0ODYyODEwNy4xNzI3MDA4MTYz*_ga*NzYyNzgzODUwLjE3MjcwMDgxNjM.*_ga_7J0VYE6519*MTcyNzAwODE2Mi4xLjEuMTcyNzAwODMwNS4wLjAuMzU2MzIzODM.&_ga=2.34506345.936241397.1727008163-762783850.1727008163#bma044f9e3-6053-4fd4-93f8-f71d163ea918 which can be purchased from nsh.org Sent from my iPhone On Sep 21, 2024, at 7:25?PM, Naira Margaryan via Histonet wrote: ?Hello, Could you please share your processing programs to run mouse, rat, rabbit, dog?s organs after full necropsy? Thank you in advance for sharing your different programs, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!y_3KqlpCSlpHMaqGsnmHjKF1WsU64BOFycExt_kkbXd92cHubjAH_WFarjsKS-vWK_2IoVXJC9PnvgO2Ul4koMk0Wtk6bQ$ From gu.lang at gmx.at Mon Sep 23 10:46:32 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Mon, 23 Sep 2024 17:46:32 +0200 Subject: [Histonet] issues with printmate Message-ID: <000901db0dcf$c40e3b60$4c2ab220$@gmx.at> Dear all! I hope someone can give me a hint with my problem. Our printmate cassette-printer was sent to repair because of an old printhead and came back with the lost of his barcode-scan-function. Obviously our technical support is not competent enough to fix the problem. Usually the cursor is placed in the barcode-field and after the scan of our entry-number the figures are split into the responding fields. Now, the number can be scanned, and is filled in the barcode-field, but the splitting doesn't happen. We have a second device and I have tried to compare the settings. And have found no difference. Maybe I have overlooked something. Thanks in advance Gudrun Lang From relia1 at earthlink.net Mon Sep 23 11:35:19 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 23 Sep 2024 12:35:19 -0400 Subject: [Histonet] =?utf-8?q?=F0=9F=8C=9F_Discover_Amazing_Opportunities_?= =?utf-8?q?in_MA=2C_FL=2C_WA=2C_MT=2C_TN=2C_VA_and_AZ__You_Won?= =?utf-8?b?4oCZdCBGaW5kIFRoZXNlIEVsc2V3aGVyZSEg8J+Mnw==?= Message-ID: <02dd01db0dd6$9517a9c0$bf46fd40$@earthlink.net> Histopeeps!!! ? Discover Amazing Opportunities You Won?t Find Elsewhere! ? These are RELIA EXCLUSIVES! My clients are offering FANTASTIC opportunities to grow, learn, mentor, train, and advance! Showcase your skills and expand your expertise. ?? My Hottest Opportunities are Located in: * MASSACHUSETTS: BRAND NEW LAB! ? * FLORIDA: North Central, Southwest, and South Florida! ? * WASHINGTON: Seattle!! ?? All of these opportunities are full-time and permanent, with excellent compensation, benefits, and relocation/sign-on bonuses. ?? I also have great opportunities in: * Tennessee * Virginia * California * Wisconsin * Arizona * Montana ? My phone and email are buzzing with labs looking for amazing histotechs like you! If you are considering a move now or in 2025, we need to chat. ? Know someone who?s looking? You can earn a referral bonus if you refer them to me and I place them! ? For more information, contact me: * Direct message me on social media * Cell/Text: 407-353-5070 ? * Email: relia1 at earthlink.net ? Have a Great DAY! Pam ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #2024NSHConvention #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? From durmtissuebank01 at gmail.com Mon Sep 23 12:31:08 2024 From: durmtissuebank01 at gmail.com (Ni Nie) Date: Mon, 23 Sep 2024 13:31:08 -0400 Subject: [Histonet] HSV1/2 Trepanonoma tissue blocks Message-ID: Our client performing research on virus identification is in urgent need of HSV1/2 and Treponema tissue blocks. We have over 30,000 tissue blocks in our inventory that we would be able to trade including rare H-Pylori and Acid Fast, as well as rare tumors with complete Pathology reports. If you are a professional tissue bank, we would also be willing to purchase these blocks from you. Ni Nie PhD Durham Tissue Bank durmtissuebank01 at gmail.com From relia1 at earthlink.net Tue Sep 24 11:18:24 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 24 Sep 2024 12:18:24 -0400 Subject: [Histonet] FREE Resume Tune UPs and Exciting RELIA Exclusives in Nashville, Boston Suburbs, Ft. Lauderdale and Seattle! Message-ID: <000601db0e9d$625b3340$271199c0$@earthlink.net> Hi Histopeeps! How are you today? ? I hope you?re having a fantastic day! It?s that time again!!! RESUME TUNE-UPS and Exciting Opportunities! ? Need a resume tune-up? I can help! Just drop me an email at relia1 at earthlink.net with a copy of your resume, and I?ll take a look at it FREE of charge! ?? And here are some REALLY fantastic job opportunities!!! My phone has been ringing off the hook since Labor Day! ? If you or anyone you know is interested in more details, reach out to me ASAP on my cell at 407-353-5070 or toll-free at 866-607-3542, or email me at relia1 at earthlink.net. Remember If you?re starting to budget for Christmas shopping, I pay a referral fee if I place someone you refer to me. ?? Here are the current opportunities that I am most excited about: Newest Hottest Opportunities: * TN: Nashville - HT/HTL - $10K sign-on plus relocation! ??? * MA: Boston Suburbs - Research AND Clinical! ?? * WA: Seattle - Derm and DAYS! ?? * FL: Ft. Lauderdale - Dermpath DAYS! ?? * AZ: Sierra Vista - Derm - Lead Tech ?? Histopeeps!! my phone is ringing off the hook with new opportunities nationwide, so let me know if you have another area in mind. ?? Remember, all of these positions are permanent and full-time with excellent benefits. In most cases, clients are assisting with relocation and offering sign-on bonuses! ?? All of them are eager to interview and hire YOU! ? Having Fun at in NOLA? Continue the fun at home networking with your fellow histopeeps in my Facebook Group: www.facebook.com/groups/histotechnologists Thanks and have a great day! Pam ?Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Follow my hashtags to make your DAYS great and your career greater! #2024NSHConvention #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From rsrichmond at gmail.com Tue Sep 24 14:46:59 2024 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 24 Sep 2024 15:46:59 -0400 Subject: [Histonet] Treponema tissue blocks Message-ID: In reply to Ni Nie's request: The best source of Treponema pallidum control tissue used to be necrotic liver from necropsies of syphilitic stillbirths. These were only 25 years in the past when I was a pathology resident in the 1960s. Possibly some of this material is still around. As congenital syphilis returns, there might be such material available today - one such case could supply the world for years, after all. Mycobacterium tuberculosis control tissue can be obtained from necropsies on monkeys with tuberculosis, most commonly rhesus macaques in India. Bob Richmond Maryville TN From relia1 at earthlink.net Wed Sep 25 11:32:47 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 25 Sep 2024 12:32:47 -0400 Subject: [Histonet] RELIA HOT JOB ALERT!!! Exclusive Opportunity in Seattle!! Message-ID: <004301db0f68$8fa0b110$aee21330$@earthlink.net> Hi Histonetters! I hope you?re having a fantastic day! ? I?m reaching out because? I have an exciting new opportunity in Seattle for a Histology Tech. This is a full-time, permanent position with a Dermatopathology Lab, working Monday to Friday during the day. Key Highlights: ? Experience with Mohs and/or IHC is a plus, but you can also learn these skills on the job! ? ? If you?re passionate about IHC, you?ll have the chance to automate the lab?s IHC department. ? ? Use your histology skills to contribute, grow, and learn in a supportive environment. ? Why This Opportunity Stands Out: ? My client is looking for amazing histotechs like you! ? ? Permanent full-time positions with excellent compensation. ? ? Flexibility to finish your travel assignment and take your vacation time. ???? Interested? Contact ME To Get More Information Or Refer A Friend! ? If you or someone you know is interested, please contact me at: Toll-free: 866-607-3542 ? Cell/Text: 407-353-5070 ? Email: relia1 at earthlink.net ? Histonetters, Even if you?re contemplating a move to a different area, let me know. I receive calls for histology professionals at all levels almost daily! ? I truly appreciate you taking the time to read this email. As a token of my gratitude, I?m offering a $250 referral fee for anyone you refer to me that I place. ? Even if you?re contemplating a move to a different area, let me know. I receive calls for histology professionals at all levels almost daily! ? Join your fellow histopeeps for fun and information in my Facebook group: www.facebook.com/groups/histotechnologists ? Have a great day! ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thanks, Pam Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407) 657-2027 Cell: (407) 353-5070 Email: relia1 at earthlink.net Facebook | LinkedIn Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Mon Sep 30 11:30:22 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 30 Sep 2024 12:30:22 -0400 Subject: [Histonet] #MotivationMonday Message-ID: <023401db1356$0caea950$260bfbf0$@earthlink.net> Happy Monday, Histopeeps! ? My go-to hashtag for Mondays? #MotivationMonday! ? New day, new beginnings, fresh outlook, and a fresh attitude. What motivation do you need to kickstart a fantastic week? I?m here to help! Whether it?s resumes, interviews, finding a great new job, or just a friendly ear, I?m just a call, email, or DM away! ? Call/Text: 407-353-5070 ? Email: relia1 at earthlink.net ? DM me on social media Let?s make this week amazing together! ? #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? Thanks-Pam ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ?????? From Amrit.Samra at hli.ubc.ca Mon Sep 30 12:00:19 2024 From: Amrit.Samra at hli.ubc.ca (Amrit Samra) Date: Mon, 30 Sep 2024 10:00:19 -0700 Subject: [Histonet] Histonet Digest, Vol 250, Issue 14 (Away) In-Reply-To: <63DC21C00200008B803483FE@mail.hli.ubc.ca> References: <63DC21C00200008B803483FE@mail.hli.ubc.ca> Message-ID: <66FAD9230200008D000C8A72@mail.hli.ubc.ca> I will be away form September 20th to Sept 27th inclusive. Thank you and see you when I get back :) Amrit From dlh3878 at gmail.com Mon Sep 30 13:24:21 2024 From: dlh3878 at gmail.com (Donielle Hunter) Date: Mon, 30 Sep 2024 14:24:21 -0400 Subject: [Histonet] ISO HSV control blocks Message-ID: Good afternoon from Ohio. My GI lab is in need of HSV control blocks. We are hoping to have a block donated in lieu of slides so we can section it out for more use rather than having to purchase slides. If anyone is able to help please respond. Thanks in advance, DL Hunter