From afhenwood at outlook.com Wed May 1 02:21:17 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Wed, 1 May 2024 07:21:17 +0000 Subject: [Histonet] Histonet Digest, Vol 245, Issue 20 In-Reply-To: References: Message-ID: A few years ago there was an article on the Block - "What is Denatured Alcohol and What are the Implications For Histopathology?" that might be useful. The URL is https://www.nsh.org/blogs/tony-henwood/2020/02/11/what-is-denatured-alcohol-and-what-are-the-implica?_gl=1*1mgiiv6*_ga*MTkyMzY5MTQ4Mi4xNzExNDA1ODQ2*_ga_7J0VYE6519*MTcxNDU0NzgyNy4xMC4xLjE3MTQ1NDc4NzcuMC4xLjEzNzYzNTQ4NjQ.&_ga=2.47649445.329273616.1714547828-1923691482.1711405846 Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: John O?Brien via Histonet Sent: 01 May 2024 01:49 To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 245, Issue 20 Ethyl Alcohol, Reagent 100% alcohol is what is normally used in Pathology processing and slide staining IMEB Inc offer all grades of Reagent alcohol, pure ethyl is a controlled alcohol regulated and taxed by government Regards John > On Apr 30, 2024, at 10:18?AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Alcohol (Naira Margaryan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 29 Apr 2024 18:49:04 -0500 > From: Naira Margaryan > To: Histonet > Subject: [Histonet] Alcohol > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hello, > > What type of ALCOHOL ETHYL you?re using in your histology lab.? > > We are thinking to get 20-30-gal drum. > > Could you please help me with that? > > > > Your suggestion is appreciated, > > Naira > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 245, Issue 20 > ***************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Wed May 1 13:12:45 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Wed, 1 May 2024 18:12:45 +0000 Subject: [Histonet] Alcohol Message-ID: For 50 yrs I have used Industrial methylated spirit ( 74OP IMS) It is cheaper than ethyl alcohol....does the same job as it is ethyl alcohol containing a part of methyl alcohol No duty charged Until recently I would buy 25 L drum Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From relia1 at earthlink.net Mon May 6 13:42:12 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 6 May 2024 14:42:12 -0400 Subject: [Histonet] RELIA WAY OUT WEST Hot Job Alert! Message-ID: <04f001da9fe5$1d095910$571c0b30$@earthlink.net> RELIA WAY OUT WEST Hot Job Alert! Hey Histopeeps! I know there are a couple of you out there who have said "If you ever get a permanent position in ________ Let ME know!" Well if that place happened to be ****MONTANA!***** I Got YOU! I have an amazing opportunity in Montana it is a full time permanent day shift position in a private lab. OR How about: ****Arizona**** ****South Dakota**** ****California***** For more info: Contact me - Pam Barker **Msg me on Social Media** **email relia1 at earthlink.net** **cell/text 407-353-5070** From criley at udel.edu Wed May 8 08:43:24 2024 From: criley at udel.edu (Charles Riley) Date: Wed, 8 May 2024 09:43:24 -0400 Subject: [Histonet] Ihc control tissues Message-ID: Hello all, I am trying to put together a positive and negative tissue control list for the following antibodies at my research core. I came over from a clinical pathology background and we used tonsils for a lot of these antibodies. Since rats and mice don't have tonsils I am looking for something they do have that can be used if possible to keep the controls as similar to the samples as possible. BCL-2 Caspase-3 CD117 CD62E (e-selectin) Collagen II Desmin GFAP HMB45 Ki67 p53 s100 From vtolley25 at gmail.com Wed May 8 09:52:23 2024 From: vtolley25 at gmail.com (Val T) Date: Wed, 8 May 2024 07:52:23 -0700 Subject: [Histonet] Ihc control tissues In-Reply-To: References: Message-ID: <0C6A1D8C-B5A9-40D8-B088-4AD2FAA4E68B@gmail.com> Hey Charles- Our research lab prepares a ?multimouse? control. We embed 10 organs on the same slide and it serves as a +/- control for just about every IHC marker. However, if you don?t want to do that- here are some tissues that would make nice controls. > BCL-2- spleen, lymph nodes > Caspase-3- spleen, lymph nodes > CD117- skin, spleen, brain, lymph nodes > CD62E (e-selectin), bone marrow, colon > Collagen II, cartilage > Desmin, heart, skeletal muscle > GFAP, brain, spinal cord > HMB45, skin > Ki67, spleen, bowel > p53, tumors, colon > s100, colon, muscle Brain can serve as a negative control on just about all these. CD117 will have some expression in the cerebellum, but will be negative in most other areas. Heart can be a negative control for GFAP. Val > On May 8, 2024, at 6:59?AM, Charles Riley via Histonet wrote: > > ?Hello all, > > I am trying to put together a positive and negative tissue control list for > the following antibodies at my research core. I came over from a clinical > pathology background and we used tonsils for a lot of these antibodies. > Since rats and mice don't have tonsils I am looking for something they do > have that can be used if possible to keep the controls as similar to the > samples as possible. > > BCL-2 > Caspase-3 > CD117 > CD62E (e-selectin) > Collagen II > Desmin > GFAP > HMB45 > Ki67 > p53 > s100 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at udel.edu Wed May 8 09:55:48 2024 From: criley at udel.edu (Charles Riley) Date: Wed, 8 May 2024 10:55:48 -0400 Subject: [Histonet] Ihc control tissues In-Reply-To: <0C6A1D8C-B5A9-40D8-B088-4AD2FAA4E68B@gmail.com> References: <0C6A1D8C-B5A9-40D8-B088-4AD2FAA4E68B@gmail.com> Message-ID: Awesome thank you Val. I like the multi control option. Can save money in the end by using less reagents On Wed, May 8, 2024 at 10:52?AM Val T wrote: > Hey Charles- > > Our research lab prepares a ?multimouse? control. We embed 10 organs on > the same slide and it serves as a +/- control for just about every IHC > marker. > However, if you don?t want to do that- here are some tissues that would > make nice controls. > > > BCL-2- spleen, lymph nodes > > Caspase-3- spleen, lymph nodes > > CD117- skin, spleen, brain, lymph nodes > > CD62E (e-selectin), bone marrow, colon > > Collagen II, cartilage > > Desmin, heart, skeletal muscle > > GFAP, brain, spinal cord > > HMB45, skin > > Ki67, spleen, bowel > > p53, tumors, colon > > s100, colon, muscle > > Brain can serve as a negative control on just about all these. CD117 will > have some expression in the cerebellum, but will be negative in most other > areas. > Heart can be a negative control for GFAP. > Val > > > On May 8, 2024, at 6:59?AM, Charles Riley via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > ?Hello all, > > > > I am trying to put together a positive and negative tissue control list > for > > the following antibodies at my research core. I came over from a > clinical > > pathology background and we used tonsils for a lot of these antibodies. > > Since rats and mice don't have tonsils I am looking for something they do > > have that can be used if possible to keep the controls as similar to the > > samples as possible. > > > > BCL-2 > > Caspase-3 > > CD117 > > CD62E (e-selectin) > > Collagen II > > Desmin > > GFAP > > HMB45 > > Ki67 > > p53 > > s100 > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cforster at umn.edu Wed May 8 12:46:24 2024 From: cforster at umn.edu (Colleen Forster) Date: Wed, 8 May 2024 12:46:24 -0500 Subject: [Histonet] Ihc control tissues In-Reply-To: References: <0C6A1D8C-B5A9-40D8-B088-4AD2FAA4E68B@gmail.com> Message-ID: Charles, I am in a research only lab as well. We create TMA's as control blocks using the same idea that Val uses. Multiple tissues per block for all IHC and also good for special stains. You can make nicely organized TMA's manually very cost effectively as well. Feel free to reach out to me if interested in more information. Colleen Forster HT(ASCP)QIHC cforster at umn.edu On Wed, May 8, 2024 at 9:56?AM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Awesome thank you Val. I like the multi control option. Can save money in > the end by using less reagents > > On Wed, May 8, 2024 at 10:52?AM Val T wrote: > > > Hey Charles- > > > > Our research lab prepares a ?multimouse? control. We embed 10 organs on > > the same slide and it serves as a +/- control for just about every IHC > > marker. > > However, if you don?t want to do that- here are some tissues that would > > make nice controls. > > > > > BCL-2- spleen, lymph nodes > > > Caspase-3- spleen, lymph nodes > > > CD117- skin, spleen, brain, lymph nodes > > > CD62E (e-selectin), bone marrow, colon > > > Collagen II, cartilage > > > Desmin, heart, skeletal muscle > > > GFAP, brain, spinal cord > > > HMB45, skin > > > Ki67, spleen, bowel > > > p53, tumors, colon > > > s100, colon, muscle > > > > Brain can serve as a negative control on just about all these. CD117 will > > have some expression in the cerebellum, but will be negative in most > other > > areas. > > Heart can be a negative control for GFAP. > > Val > > > > > On May 8, 2024, at 6:59?AM, Charles Riley via Histonet < > > histonet at lists.utsouthwestern.edu> wrote: > > > > > > ?Hello all, > > > > > > I am trying to put together a positive and negative tissue control list > > for > > > the following antibodies at my research core. I came over from a > > clinical > > > pathology background and we used tonsils for a lot of these antibodies. > > > Since rats and mice don't have tonsils I am looking for something they > do > > > have that can be used if possible to keep the controls as similar to > the > > > samples as possible. > > > > > > BCL-2 > > > Caspase-3 > > > CD117 > > > CD62E (e-selectin) > > > Collagen II > > > Desmin > > > GFAP > > > HMB45 > > > Ki67 > > > p53 > > > s100 > > > _______________________________________________ > > > Histonet mailing list > > > Histonet at lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From relia1 at actsend.com Wed May 8 13:14:00 2024 From: relia1 at actsend.com (Pam Barker) Date: Wed, 08 May 2024 18:14:00 +0000 Subject: [Histonet] A Letter To My Histopeeps. I Can Keep A Secret. Can YOU? Message-ID: <1D.91.40028.8E0CB366@ic.mta1vrest.cc.prd.sparkpost> Hello?Histopeeps I hope you are having a great day. Did you know you might not even see the best opportunities? What I am seeing is about 50% of the time The "PLUM" Jobs Don't Get Posted! ???????? It's Networking! ???????? Word of Mouth! ???????? Working with Recruiters! Ask Yourself- Which option is your safest bet for a confidential search? ??????????????????????A Recruiter!???????????????????????????? The Majority Of The Opportunities I Represent Are NEVER?Advertised! These are CONFIDENTIAL Searches! Confidential Because: ? It's A New Facility ? No One On Staff Can do the job ? A Reorganization Opportunities Like: v Management - Manager, Supervisor, Lead Tech v Vendor Opportunities - Leadership, Sales, Support v Quality Assurance/Quality Control v Education When I Get One Of These Positions, I Go To The People Who Have Told Me What THEIR Next Opportunity Looks Like! AFTER I speak with them I put it in our Histopeep Career Bulletin! Histopeeps Want First DIBS? Help ME, Help YOU! Shoot me a quick email OR schedule a call and Tell Me YOUR Next Opportunity NEEDS to Look Like! Have a great day and Thanks for Taking the Time! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? Click Here to Unsubscribe RELIA Solutions histonet at lists.utsouthwestern.edu 5703 Red Bug Lake Road #330
Winter Springs, fl 32708 From relia1 at earthlink.net Wed May 8 13:14:23 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 8 May 2024 14:14:23 -0400 Subject: [Histonet] A Letter To My Histopeeps. I Can Keep A Secret. Can You? Message-ID: <000201daa173$8ec13a70$ac43af50$@earthlink.net> Hello Histopeeps! I hope you are having a great day. Did you know you might not even see the best opportunities? What I am seeing is about 50% of the time The "PLUM" Jobs Don't Get Posted! * It's Networking! * Word of Mouth! * Working with Recruiters! Ask Yourself- Which option is your safest bet for a confidential search? A Recruiter! The majority of the leadership opportunities that I work on are NEVER advertised! These are CONFIDENTIAL Searches! Confidential Because: * It's A New Facility * No One On Staff Can do the job * A Reorganization Opportunities Like: * Management - Manager, Supervisor, Lead Tech * Vendor Opportunities - Leadership, Sales, Support * Quality Assurance/Quality Control * Education When I Get One Of These Positions, I Go To The People Who Have Told Me What THEIR Next Opportunity Looks Like! After I speak with them I put it in our Histopeep Career Bulletin. Want First DIBS? Help ME, Help YOU! Shoot me a quick email OR schedule a call and Tell Me YOUR Next Opportunity NEEDS to Look Like! Have a great day and Thanks for Taking the Time! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net From igonzalezm3 at correo.uss.cl Fri May 10 19:53:25 2024 From: igonzalezm3 at correo.uss.cl (=?iso-8859-1?Q?IGNACIO_GONZ=C1LEZ_MASSONI?=) Date: Sat, 11 May 2024 00:53:25 +0000 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Message-ID: Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist From jkiernan at uwo.ca Fri May 10 23:03:53 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 11 May 2024 04:03:53 +0000 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy In-Reply-To: References: Message-ID: If you apply the ammonia to the cut surface of the paraffin block, I suspect that it softens the tissue in the same way as applying water: by entering interstices of the tissue that are not occupied by paraffin molecules. I never tried ammonia for this purpose but in the 1960s to early '70s I occasionally used a proprietary product called Mollifex, which I see is still sold. In 1972 or '73 an elderly technician told me that water was just as good, and I soon found out that he was right. Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. John Kiernan Emeritus, UWO, London, Canada https://publish.uwo.ca/~jkiernan/ = = = ________________________________ From: IGNACIO GONZ?LEZ MASSONI via Histonet Sent: May 10, 2024 8:53 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmargaryan88 at gmail.com Sat May 11 00:01:42 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Sat, 11 May 2024 00:01:42 -0500 Subject: [Histonet] Anti static for paraffin sections Message-ID: Hello, Wam interested to know what you are using to reduce static of paraffin sections. Please share your experience and expertise. Thanks in advance, Naira From jaylundgren at gmail.com Sat May 11 15:40:41 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Sat, 11 May 2024 15:40:41 -0500 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy In-Reply-To: References: Message-ID: I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > If you apply the ammonia to the cut surface of the paraffin block, I > suspect that it softens the tissue in the same way as applying water: by > entering interstices of the tissue that are not occupied by paraffin > molecules. > I never tried ammonia for this purpose but in the 1960s to early '70s I > occasionally used a proprietary product called Mollifex, which I see is > still sold. In 1972 or '73 an elderly technician told me that water was > just as good, and I soon found out that he was right. Indeed, water had the > advantage of working in 15-30 minutes rather than taking several hours. > John Kiernan > Emeritus, UWO, London, Canada > https://publish.uwo.ca/~jkiernan/ > = = = > ________________________________ > From: IGNACIO GONZ?LEZ MASSONI via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: May 10, 2024 8:53 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy > > Dear friends at Histonet, > > I hope this message finds you well. I am reaching out to seek your > expertise on a matter that has piqued my interest in the field of histology. > > I am currently delving into the process of preparing FFPE (formalin-fixed, > paraffin-embedded) tissues for microtomy. Specifically, I am curious about > the role of ammonia in softening these tissues before sectioning. From my > understanding, ammonia serves as an alkaline agent that helps neutralize > formalin's effects and facilitates the removal of paraffin, thus aiding in > the softening of the tissue. > > However, I would greatly appreciate if you could provide a more detailed > explanation of the chemical interactions at play here. How exactly does > ammonia interact with the tissue components to achieve the desired > softening effect? Moreover, are there any best practices or safety > precautions that one should be aware of when using ammonia in this context? > > Your insights on this topic would be invaluable to me and would greatly > enhance my understanding of the intricacies involved in histological > preparations. > > Thank you for your time and assistance. > > Warm regards from Santiago of Chile > > Ignacio, Medical Technologist > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan at uwo.ca Sun May 12 00:31:35 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 12 May 2024 05:31:35 +0000 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy In-Reply-To: References: Message-ID: Jay, you are 100% right about "pseudo-scientific mumbo jumbo" for the mechanism. But have you (or anyone else) compared ammonia-water with ordinary pure water for hydrating/softening? Water (with or without dissolved ammonia) won't go through solid wax. The embedded tissue has to be exposed by cutting into the block. Whiffs of ammonia are unpleasant. If they upset some people in the lab, that's a good reason to try odorless water instead. John Kiernan Emeritus, UWO, London, Canada https://publish.uwo.ca/~jkiernan/ = = = From: Jay Lundgren Sent: May 11, 2024 4:40 PM To: John Kiernan Cc: histonet at lists.utsouthwestern.edu ; IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet > wrote: If you apply the ammonia to the cut surface of the paraffin block, I suspect that it softens the tissue in the same way as applying water: by entering interstices of the tissue that are not occupied by paraffin molecules. I never tried ammonia for this purpose but in the 1960s to early '70s I occasionally used a proprietary product called Mollifex, which I see is still sold. In 1972 or '73 an elderly technician told me that water was just as good, and I soon found out that he was right. Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. John Kiernan Emeritus, UWO, London, Canada https://publish.uwo.ca/~jkiernan/ = = = ________________________________ From: IGNACIO GONZ?LEZ MASSONI via Histonet > Sent: May 10, 2024 8:53 PM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Jay Lundgren Sent: May 11, 2024 4:40 PM To: John Kiernan Cc: histonet at lists.utsouthwestern.edu ; IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet > wrote: If you apply the ammonia to the cut surface of the paraffin block, I suspect that it softens the tissue in the same way as applying water: by entering interstices of the tissue that are not occupied by paraffin molecules. I never tried ammonia for this purpose but in the 1960s to early '70s I occasionally used a proprietary product called Mollifex, which I see is still sold. In 1972 or '73 an elderly technician told me that water was just as good, and I soon found out that he was right. Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. John Kiernan Emeritus, UWO, London, Canada https://publish.uwo.ca/~jkiernan/ = = = ________________________________ From: IGNACIO GONZ?LEZ MASSONI via Histonet > Sent: May 10, 2024 8:53 PM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Sun May 12 17:37:05 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Sun, 12 May 2024 17:37:05 -0500 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy In-Reply-To: References: Message-ID: Pleasant is not a mission parameter. From afhenwood at outlook.com Sun May 12 18:22:31 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Sun, 12 May 2024 23:22:31 +0000 Subject: [Histonet] Inquiry on Tissue Softening for Microtomy In-Reply-To: References: Message-ID: My preference is cold water or Mollifex on faced blocks. Ammonia is definitely obnoxious (reminds me of wet nappies ?) Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Jay Lundgren via Histonet Sent: 13 May 2024 08:37 To: John Kiernan Cc: histonet at lists.utsouthwestern.edu ; IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy Pleasant is not a mission parameter. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dclunie at dclunie.com Mon May 13 07:13:50 2024 From: dclunie at dclunie.com (=?UTF-8?Q?David_Clunie?=) Date: Mon, 13 May 2024 12:13:50 +0000 Subject: [Histonet] Crowdsourcing codes for standard staining methods to improve digital pathology communication References: <7c717a6e-def2-4cc8-a3ae-24ced89afe31@dclunie.com> Message-ID: <0100018f71df9b4f-5ebc14ec-2d6b-4fd1-9409-da32d15c4944-000000@email.amazonses.com> There is an ongoing effort to standardize and code staining methods (esp. for use in populating DICOM WSI metadata and HL7 messages from the AP-LIS to the scanner in IHE DPIA profile). If anyone is interested in contributing, see the crowdsourced spreadsheet at: https://tinyurl.com/stainmap Please feel free to contribute. E-mail me if it is not obvious what to do or how to use the spreadsheet. David From TNMayer at mdanderson.org Tue May 14 11:25:27 2024 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 14 May 2024 16:25:27 +0000 Subject: [Histonet] [EXTERNAL] Histonet Digest, Vol 246, Issue 7 In-Reply-To: References: Message-ID: Jay, I would think that the alkaline properties of the ammonia slow the swelling of the tissues unlike plain water. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Asst Professor/ Assoc Program Director HTL Program UTMDACC tnmayer at mdanderson.edu off cell: 832-710-1837 off: 713-563-3481 * My workday may look different from your workday. Please do not feel obligated to respond outside of your normal working hours. Today's Topics: 1. Re: Inquiry on Tissue Softening for Microtomy (Jay Lundgren) 2. Re: Inquiry on Tissue Softening for Microtomy (John Kiernan) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 May 2024 15:40:41 -0500 From: Jay Lundgren To: John Kiernan Cc: "histonet at lists.utsouthwestern.edu" , IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy Message-ID: Content-Type: text/plain; charset="UTF-8" I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > If you apply the ammonia to the cut surface of the paraffin block, > I suspect that it softens the tissue in the same way as applying > water: by entering interstices of the tissue that are not occupied by > paraffin molecules. > I never tried ammonia for this purpose but in the 1960s to early > '70s I occasionally used a proprietary product called Mollifex, which > I see is still sold. In 1972 or '73 an elderly technician told me that > water was just as good, and I soon found out that he was right. > Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. > John Kiernan > Emeritus, UWO, London, Canada > https://urldefense.com/v3/__https://publish.uwo.ca/*jkiernan/__;fg!!Pf > beBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAY > jEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeH0EH0M$ > = = = > ________________________________ > From: IGNACIO GONZ?LEZ MASSONI via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: May 10, 2024 8:53 PM > To: histonet at lists.utsouthwestern.edu > > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy > > Dear friends at Histonet, > > I hope this message finds you well. I am reaching out to seek your > expertise on a matter that has piqued my interest in the field of histology. > > I am currently delving into the process of preparing FFPE > (formalin-fixed, > paraffin-embedded) tissues for microtomy. Specifically, I am curious > about the role of ammonia in softening these tissues before > sectioning. From my understanding, ammonia serves as an alkaline agent > that helps neutralize formalin's effects and facilitates the removal > of paraffin, thus aiding in the softening of the tissue. > > However, I would greatly appreciate if you could provide a more > detailed explanation of the chemical interactions at play here. How > exactly does ammonia interact with the tissue components to achieve > the desired softening effect? Moreover, are there any best practices > or safety precautions that one should be aware of when using ammonia in this context? > > Your insights on this topic would be invaluable to me and would > greatly enhance my understanding of the intricacies involved in > histological preparations. > > Thank you for your time and assistance. > > Warm regards from Santiago of Chile > > Ignacio, Medical Technologist > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1 > f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1 > f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ > ------------------------------ Message: 2 Date: Sun, 12 May 2024 05:31:35 +0000 From: John Kiernan To: Jay Lundgren Cc: "histonet at lists.utsouthwestern.edu" , IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy Message-ID: Content-Type: text/plain; charset="utf-8" Jay, you are 100% right about "pseudo-scientific mumbo jumbo" for the mechanism. But have you (or anyone else) compared ammonia-water with ordinary pure water for hydrating/softening? Water (with or without dissolved ammonia) won't go through solid wax. The embedded tissue has to be exposed by cutting into the block. Whiffs of ammonia are unpleasant. If they upset some people in the lab, that's a good reason to try odorless water instead. John Kiernan Emeritus, UWO, London, Canada https://urldefense.com/v3/__https://publish.uwo.ca/*jkiernan/__;fg!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeH0EH0M$ = = = From: Jay Lundgren Sent: May 11, 2024 4:40 PM To: John Kiernan Cc: histonet at lists.utsouthwestern.edu ; IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet > wrote: If you apply the ammonia to the cut surface of the paraffin block, I suspect that it softens the tissue in the same way as applying water: by entering interstices of the tissue that are not occupied by paraffin molecules. I never tried ammonia for this purpose but in the 1960s to early '70s I occasionally used a proprietary product called Mollifex, which I see is still sold. In 1972 or '73 an elderly technician told me that water was just as good, and I soon found out that he was right. Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. John Kiernan Emeritus, UWO, London, Canada https://urldefense.com/v3/__https://publish.uwo.ca/*jkiernan/__;fg!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeH0EH0M$ = = = ________________________________ From: IGNACIO GONZ?LEZ MASSONI via Histonet > Sent: May 10, 2024 8:53 PM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ ________________________________ From: Jay Lundgren Sent: May 11, 2024 4:40 PM To: John Kiernan Cc: histonet at lists.utsouthwestern.edu ; IGNACIO GONZ?LEZ MASSONI Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. Go soak a solid block of paraffin in pure NH4OH for 24 hours, it won't do anything. I was told as a student at AFIP, "It opens the pores of the tissue so water can get in." In other words, pseudo-scientific mumbo jumbo. It works though. Somebody needs to get a $150,000,000 NIH grant and do a research project on how ammonia water hydrates tissue. I worked with some lovely Hmong people in California that called it "crying water". Using it can cause a big interpersonal problem with certain people in the lab though. Interestingly, hypersensitivity to smells is one of the symptoms of autism. Hypersensitivity to smells is also highly correlated with bipolar disorder and heightened emotional reactivity. Sooo... Jay A. Lundgren, M.S., HTL (ASCP) On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet > wrote: If you apply the ammonia to the cut surface of the paraffin block, I suspect that it softens the tissue in the same way as applying water: by entering interstices of the tissue that are not occupied by paraffin molecules. I never tried ammonia for this purpose but in the 1960s to early '70s I occasionally used a proprietary product called Mollifex, which I see is still sold. In 1972 or '73 an elderly technician told me that water was just as good, and I soon found out that he was right. Indeed, water had the advantage of working in 15-30 minutes rather than taking several hours. John Kiernan Emeritus, UWO, London, Canada https://urldefense.com/v3/__https://publish.uwo.ca/*jkiernan/__;fg!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeH0EH0M$ = = = ________________________________ From: IGNACIO GONZ?LEZ MASSONI via Histonet > Sent: May 10, 2024 8:53 PM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Inquiry on Tissue Softening for Microtomy Dear friends at Histonet, I hope this message finds you well. I am reaching out to seek your expertise on a matter that has piqued my interest in the field of histology. I am currently delving into the process of preparing FFPE (formalin-fixed, paraffin-embedded) tissues for microtomy. Specifically, I am curious about the role of ammonia in softening these tissues before sectioning. From my understanding, ammonia serves as an alkaline agent that helps neutralize formalin's effects and facilitates the removal of paraffin, thus aiding in the softening of the tissue. However, I would greatly appreciate if you could provide a more detailed explanation of the chemical interactions at play here. How exactly does ammonia interact with the tissue components to achieve the desired softening effect? Moreover, are there any best practices or safety precautions that one should be aware of when using ammonia in this context? Your insights on this topic would be invaluable to me and would greatly enhance my understanding of the intricacies involved in histological preparations. Thank you for your time and assistance. Warm regards from Santiago of Chile Ignacio, Medical Technologist _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$ ------------------------------ End of Histonet Digest, Vol 246, Issue 7 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From tkngflght at yahoo.com Tue May 14 16:13:42 2024 From: tkngflght at yahoo.com (Cheryl) Date: Tue, 14 May 2024 16:13:42 -0500 Subject: [Histonet] De-mummification procedure? References: Message-ID: Hi and help? A tech on another forum is dealing with a catastrophe. Does anyone have the glycerin de-mummification procedure? Haven?t used it in decades but with what those samples went through it may be their last chance as any sort of readable slides. TIA!! Cheryl Please excuse typos-sent from a phone. From jaylundgren at gmail.com Wed May 15 14:06:05 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 15 May 2024 14:06:05 -0500 Subject: [Histonet] De-mummification procedure? In-Reply-To: References: Message-ID: The Mummy's major weakness is fire, a common weakness among the undead. Since mummies tend to be dry and coated with various oils and resins, the revenant tends to burn very well. Thus fire is the only way to destroy the Mummy forever (aka de-mummification). Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, May 14, 2024 at 4:21?PM Cheryl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi and help? A tech on another forum is dealing with a catastrophe. > > Does anyone have the glycerin de-mummification procedure? Haven?t used it > in decades but with what those samples went through it may be their last > chance as any sort of readable slides. > > TIA!! > > Cheryl > > > Please excuse typos-sent from a phone. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From SThompson4 at sonichealthcareusa.com Wed May 15 14:35:33 2024 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Wed, 15 May 2024 19:35:33 +0000 Subject: [Histonet] Histology Supervisor Opportunity Message-ID: <51f5d615fd1440599c8bfd0453c38329@sonichealthcareusa.com> Come to the Big Apple, New York, New York!! SONIC HEALTHCARE USA IS LOOKING FOR A HISTOLOGY SUPERVISOR Location: Rye Brook, NY Days: Monday - Friday Hours: 8:00 AM - 5:00 PM Full-time: Benefit Eligible In this role, you will: Manage day to day operation of the histology department (limited processing laboratory). Assign, oversee, and review the work of employees. * Manage the day to day operations of the department. This includes: * Staff supervision including but not limited to training, competency assessment * Perform interviews and all hiring * Annual employee performance evaluations * Produce, monitor and maintain productivity, quality and TAT metrics * Prepare and revise SOP's and manuals * Oversee and perform histology work - grossing, embedding, cutting, staining, QA * Maintain all quality and service standards * Adhere to department budget * Validation of methodology and instrumentation. * Laboratory Inspection and preparation All you need is: * BS/BA Degree with minimum 1-3 years of histology supervisory experience. Must meet all state and local licensing requirements where the laboratory is located (NY State) and must meet all College of American Pathologists (CAP) and CLIA requirements. (HT)ASCP or similar nationally recognized certification preferred. * NYS License Company: Sonic Anatomic Pathology We'll give you: * Appreciation for your work * A feeling of satisfaction that you've helped people * Opportunity to grow in your profession * Free lab services for you and your dependents * Work-life balance, including Paid Time Off and Paid Holidays * Competitive benefits including medical, dental, and vision insurance * Help saving for retirement, with a 401(k) plus a company match * A sense of belonging - we're a community! We also want you to know: This role will have routine access to Protected Health Information (PHI). Employees will be trained on reasonable safeguards and are expected to maintain strict confidentiality, as well as abide by all applicable privacy and security standards. Employees are expected only to access PHI when it is required to fulfill job duties. Salary Range: $95,000 - $112,000/annual will be based on commensurate with experience; geographic differentials to the pay range may apply. Please contact: Stephanie Thompson, sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From Johanna.Haddock at propath.com Thu May 16 16:16:37 2024 From: Johanna.Haddock at propath.com (Johanna Haddock) Date: Thu, 16 May 2024 21:16:37 +0000 Subject: [Histonet] Paraffin Dispenser- Temp Set up In-Reply-To: References: Message-ID: Hello, CAP ANP.23130 states, "The temperature of the paraffin dispenser and baths must be correct for the type of paraffin used." Are you correcting the paraffin dispenser temperature to match your paraffin product specifications (i.e. melting point of 50-54 ?C)? Thanks, Johanna Haddock MBA, MT(ASCP) Director, Quality Management/Safety officer | Sonic Healthcare USA Anatomic Pathology Division Sonic Healthcare USA (Anatomic Pathology Division) P 214.237.1623 | F 214-237-1731 | A 1355 River Bend Drive, Dallas, TX 75247 http://www.propath.com/ E-MAIL CONFIDENTIALITY NOTICE: The information transmitted in this e-mail and in any replies and forwards are for the sole use of the above individual(s) or entities and may contain proprietary, privileged and/or highly confidential information. Any unauthorized dissemination, review, distribution or copying of these communications is strictly prohibited. If this e-mail has been transmitted to you in error, please notify and return the original message to the sender immediately at the above listed address. Thank you for your cooperation. -----Original Message----- From: Jay Lundgren via Histonet Sent: Tuesday, April 30, 2024 12:08 PM To: Naira Margaryan Cc: Histonet Subject: Re: [Histonet] Alcohol EXTERNAL EMAIL: This email originated from outside of the organization. Do not click any links or open any attachments unless you trust the sender and know the content is safe. Call StatLab, 1-800-442-3573 On Mon, Apr 29, 2024 at 6:49?PM Naira Margaryan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > What type of ALCOHOL ETHYL you?re using in your histology lab.? > > We are thinking to get 20-30-gal drum. > > Could you please help me with that? > > > > Your suggestion is appreciated, > > Naira > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists/ > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjoh > anna.haddock%40propath.com%7C671c7fec62094410c7ca08dc693a264a%7Ceab7e4 > b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638500945681052995%7CUnknown%7CTW > FpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6 > Mn0%3D%7C0%7C%7C%7C&sdata=n5V5UEmZHR8uhshBqKXbtBaiQ%2BFJQIoL2xZ0BnnrAB > 4%3D&reserved=0 > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Thu May 16 23:16:47 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Fri, 17 May 2024 04:16:47 +0000 (UTC) Subject: [Histonet] De-mummification procedure? In-Reply-To: References: Message-ID: <920715350.912439.1715919407202@mail.yahoo.com> Jay, Groaning and eye rolling..... Sincerely, Paula Sicurello On Wednesday, May 15, 2024 at 12:06:24 PM PDT, Jay Lundgren via Histonet wrote: The Mummy's major weakness is fire, a common weakness among the undead. Since mummies tend to be dry and coated with various oils and resins, the revenant tends to burn very well.? Thus fire is the only way to destroy the Mummy forever (aka de-mummification). Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, May 14, 2024 at 4:21?PM Cheryl via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi and help?? A tech on another forum is dealing with a catastrophe. > > Does anyone have the glycerin de-mummification procedure?? Haven?t used it > in decades but with what those samples went through it may be their last > chance as any sort of readable slides. > > TIA!! > > Cheryl > > > Please excuse typos-sent from a phone. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christopher-otto at uiowa.edu Fri May 17 12:04:15 2024 From: christopher-otto at uiowa.edu (Otto, Christopher M) Date: Fri, 17 May 2024 17:04:15 +0000 Subject: [Histonet] Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: Hello everyone! I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block. I've chilled the blocks and I'm sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge blade. The paraffin surrounding the agarose sections normally, but the agarose portion of the block causes the blade to "skip" across it slightly and even chip out as if my blade isn't snug in the blade holder (it is). If I do get a tiny portion of agarose on my section to float out on the waterbath it flies away (like adding ETOH to a waterbath with sections already on it.) Everyone I have asked about this says the agarose should section normally with the paraffin like any other FFPE blocks. Any ideas on why this agarose is behaving this way for me? Thank you in advance! From jaylundgren at gmail.com Fri May 17 14:31:44 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 17 May 2024 14:31:44 -0500 Subject: [Histonet] Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: Where did the agarose come from? On Fri, May 17, 2024 at 12:09?PM Otto, Christopher M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > Hello everyone! > > I'm having trouble sectioning tissue array blocks where the array is in > agarose embedded into a paraffin block. I've chilled the blocks and I'm > sectioning on a rotary microtome, at 5 microns, with a high profile > Accuedge blade. The paraffin surrounding the agarose sections normally, but > the agarose portion of the block causes the blade to "skip" across it > slightly and even chip out as if my blade isn't snug in the blade holder > (it is). If I do get a tiny portion of agarose on my section to float out > on the waterbath it flies away (like adding ETOH to a waterbath with > sections already on it.) Everyone I have asked about this says the > agarose should section normally with the paraffin like any other FFPE > blocks. Any ideas on why this agarose is behaving this way for me? Thank > you in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From christopher-otto at uiowa.edu Fri May 17 15:05:48 2024 From: christopher-otto at uiowa.edu (Otto, Christopher M) Date: Fri, 17 May 2024 20:05:48 +0000 Subject: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: I didn't take part in the preparation of the agarose or the processing. Was pretty much just handed the blocks and asked to make slides. Get Outlook for Android ________________________________ From: Jay Lundgren Sent: Friday, May 17, 2024 2:31:44 PM To: Otto, Christopher M Cc: histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block You don't often get email from jaylundgren at gmail.com. Learn why this is important Where did the agarose come from? On Fri, May 17, 2024 at 12:09?PM Otto, Christopher M via Histonet > wrote: Hello everyone! I'm having trouble sectioning tissue array blocks where the array is in agarose embedded into a paraffin block. I've chilled the blocks and I'm sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge blade. The paraffin surrounding the agarose sections normally, but the agarose portion of the block causes the blade to "skip" across it slightly and even chip out as if my blade isn't snug in the blade holder (it is). If I do get a tiny portion of agarose on my section to float out on the waterbath it flies away (like adding ETOH to a waterbath with sections already on it.) Everyone I have asked about this says the agarose should section normally with the paraffin like any other FFPE blocks. Any ideas on why this agarose is behaving this way for me? Thank you in advance! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Fri May 17 15:06:34 2024 From: cforster at umn.edu (Colleen Forster) Date: Fri, 17 May 2024 15:06:34 -0500 Subject: [Histonet] Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32?PM Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Where did the agarose come from? > > On Fri, May 17, 2024 at 12:09?PM Otto, Christopher M via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > > > > Hello everyone! > > > > I'm having trouble sectioning tissue array blocks where the array is in > > agarose embedded into a paraffin block. I've chilled the blocks and I'm > > sectioning on a rotary microtome, at 5 microns, with a high profile > > Accuedge blade. The paraffin surrounding the agarose sections normally, > but > > the agarose portion of the block causes the blade to "skip" across it > > slightly and even chip out as if my blade isn't snug in the blade holder > > (it is). If I do get a tiny portion of agarose on my section to float > out > > on the waterbath it flies away (like adding ETOH to a waterbath with > > sections already on it.) Everyone I have asked about this says the > > agarose should section normally with the paraffin like any other FFPE > > blocks. Any ideas on why this agarose is behaving this way for me? Thank > > you in advance! > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From christopher-otto at uiowa.edu Mon May 20 07:47:11 2024 From: christopher-otto at uiowa.edu (Otto, Christopher M) Date: Mon, 20 May 2024 12:47:11 +0000 Subject: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: Hi Colleen, they are blocks of spheroids so they are pretty small. I don't know how they were processed or how the agarose was prepared. The samples you are talking about, that you ran a short run on, how did that behave when you tried to section on the microtome? Thanks -Chris ________________________________ From: Colleen Forster Sent: Friday, May 17, 2024 3:06 PM To: Jay Lundgren Cc: Otto, Christopher M ; histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block You don't often get email from cforster at umn.edu. Learn why this is important Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32?PM Jay Lundgren via Histonet > wrote: Where did the agarose come from? On Fri, May 17, 2024 at 12:09?PM Otto, Christopher M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > > Hello everyone! > > I'm having trouble sectioning tissue array blocks where the array is in > agarose embedded into a paraffin block. I've chilled the blocks and I'm > sectioning on a rotary microtome, at 5 microns, with a high profile > Accuedge blade. The paraffin surrounding the agarose sections normally, but > the agarose portion of the block causes the blade to "skip" across it > slightly and even chip out as if my blade isn't snug in the blade holder > (it is). If I do get a tiny portion of agarose on my section to float out > on the waterbath it flies away (like adding ETOH to a waterbath with > sections already on it.) Everyone I have asked about this says the > agarose should section normally with the paraffin like any other FFPE > blocks. Any ideas on why this agarose is behaving this way for me? Thank > you in advance! > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From b-frederick at northwestern.edu Mon May 20 08:00:02 2024 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Mon, 20 May 2024 13:00:02 +0000 Subject: [Histonet] Agarose embedded tissue arrays embedded in paraffin block In-Reply-To: References: Message-ID: Exactly, I process the agar,not the tissue in it.... Cells,organoids, spheroids. Bernice -----Original Message----- From: Colleen Forster via Histonet Sent: Friday, May 17, 2024 3:07 PM To: Jay Lundgren Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block Chistopher, Were these small pieces of tissue? If they are in an agar (such as histogel) they would need to have been processed overnight to ensure the agar is completely dehydrated during processing. The sample, no matter how small, is protected and processes perfectly. IF you ran a short run with the agar it will not have [processed properly. I was never able to salvage those samples. It took me a couple times to figure out the solution. Just a thought. Colleen Forster HT(ASCP)QIHC On Fri, May 17, 2024 at 2:32?PM Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Where did the agarose come from? > > On Fri, May 17, 2024 at 12:09?PM Otto, Christopher M via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > > > > > Hello everyone! > > > > I'm having trouble sectioning tissue array blocks where the array > > is in agarose embedded into a paraffin block. I've chilled the > > blocks and I'm sectioning on a rotary microtome, at 5 microns, with > > a high profile Accuedge blade. The paraffin surrounding the agarose > > sections normally, > but > > the agarose portion of the block causes the blade to "skip" across > > it slightly and even chip out as if my blade isn't snug in the blade > > holder (it is). If I do get a tiny portion of agarose on my section > > to float > out > > on the waterbath it flies away (like adding ETOH to a waterbath with > > sections already on it.) Everyone I have asked about this says the > > agarose should section normally with the paraffin like any other > > FFPE blocks. Any ideas on why this agarose is behaving this way for > > me? Thank you in advance! > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/ > > listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eR > > ALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOK > > Z8ESaaEB3125Zq$ > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/li > stinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6 > YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaa > EB3125Zq$ > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SEM0o5vbbKuzHCJUAy39eRALW6YwlWuJkF7rTMW9nJ_DRMWtgXqBRmD4yuNChwCHd4H_uCmrPk4dybLFdX60UH_YOKZ8ESaaEB3125Zq$ From relia1 at earthlink.net Mon May 20 11:29:35 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 20 May 2024 12:29:35 -0400 Subject: [Histonet] Happy Monday! What Can I Do to HELP YOU? Message-ID: <042e01daaad2$e83c4910$b8b4db30$@earthlink.net> Happy Monday Histonetters/ Histopeeps! Let's tackle this week like a caffeinated squirrel chasing after its last acorn! What's your secret superpower for surviving the start of the week? REMEMBER>>>> If there is EVER anything I can do for YOU... Resume Tune UP! Interview PREP! Curious about TRENDS? NEED Career Advice? Help with Salary Negotiations? There is NEVER a Charge For These Services Even If You Find A Job On YOUR Own! Contact ME! Msg me on Social Media Email: relia1 at earthlink.net cell/text 407-353-5070 Here are the locations on SOME Of My HOTTEST Opportunities: Leadership AND Staff Positions!! Florida Virginia South Dakota Arizona Texas Georgia Wisconsin This IS a Partial List! If you have another location in mind: LET ME KNOW! YOU Won't Find a Recruiter WHO Care More About You than ME XOXO!! All Of These Are Full Time Positions with the Cream of The Crop of Employers! Some Of These You Might Not See Elsewhere. WHY? Because They are RELIA Exclusives! My Clients Offer Excellent Compensation, Benefits and Relocation Assistance and Sign-On Bonuses Up To 20K. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Tue May 21 12:03:14 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 21 May 2024 13:03:14 -0400 Subject: [Histonet] Happy Travel Tuesday!!! Message-ID: <00fe01daaba0$c5735d00$505a1700$@earthlink.net> Happy Travel Tuesday Histonetters/Histopeeps!! The First of June is just about a week AWAY! Do YOU Have Plans THIS Summer? Traveling To Fun And Even Exotic Places? I Hope You Have A Blast! If You Happen To Visit A Place You Might Like To Live LET ME KNOW! I Might Have A Great Opportunity NOW OR Later When The Timing Is Better. Thinking About Travel Histology? Think AGAIN! Rates Are FALLING And Good Contracts Are Drying Up! On The Other Hand, Have YOU considered a PERMANENT Position? Some Of My Positions Pay Even More Than Travel! Some Of My Clients Are Offering Sign On Bonuses Up To 20k Email Me At Relia1 at Earthlink.Net Call/Text Me At 407-353-5070 Have A Great Tuesday!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at actsend.com Tue May 21 12:32:13 2024 From: relia1 at actsend.com (Pam Barker) Date: Tue, 21 May 2024 17:32:13 +0000 Subject: [Histonet] Happy Travel Tuesday! What are YOU Doing For YOUR Summer Vacation? Message-ID: Happy Travel Tuesday?Histopeeps !! The First of June is just about a week AWAY! Do YOU Have Plans THIS Summer? Traveling To Fun And Even Exotic Places? I Hope You ?Have A Blast! ?Histopeeps,?Here's A Thought>>? ? ? ? ? ? ? ? ? ? ? ? If You Happen To Visit A Place You Might Like To Live ?????????? LET ME KNOW! I Might Have A Great Opportunity NOW OR Later When The Timing Is Better. Thinking About Travel Histology? Think AGAIN! Rates Are FALLING And Good Contracts Are Drying Up! On The Other Hand, Have YOU considered a PERMANENT Position? Some Of My Positions Pay Even More Than Travel! Some Of My Clients Are Offering Sign On Bonuses Up To 20k Email Me At Relia1 at Earthlink.Net Call/Text Me At 407-353-5070 Have A Great Tuesday!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Click Here to Unsubscribe RELIA Solutions histonet at lists.utsouthwestern.edu 5703 Red Bug Lake Road #330
Winter Springs, fl 32708 From SThompson4 at sonichealthcareusa.com Tue May 21 15:28:33 2024 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 21 May 2024 20:28:33 +0000 Subject: [Histonet] Salt Lake City, Utah - Histology Technician Message-ID: Sonic Healthcare USA is hiring a Histology Technician Location: Salt Lake City, Utah - Days: Monday - Friday Hours: 5:00 AM- 1:00 PM Full-Time/Benefit Eligible In this role, you will: Perform a vital part of the patient care process through embedding and preparing slides for routine H & E and special stain procedures Performs and documents scheduled preventative maintenance Recognize when troubleshooting is needed for processing, embedding, cutting, staining Work in a fast-paced laboratory environment with biological and chemical hazards Champion safety, compliance, and quality control All you need is: At minimum graduation from a school of Histotechnology accredited by CAHEA/NAACLS AND HT (ASCP) 1 year of laboratory training or experience performing high complexity testing Certification from the American Society of Clinical Pathologists or equivalent Strong reading, writing, and analytical skills Ability to operate general laboratory equipment, including but not limited to: telephones, computers, automated analyzers, centrifuges, microscopes, manual and automated pipettes, and audible alarms We'll give you: Appreciation for your work A feeling of satisfaction that you've helped people Opportunity to grow in your profession Free lab services for you and your dependents Work-life balance, including Paid Time Off and Paid Holidays Competitive benefits including medical, dental, and vision insurance Help saving for retirement, with a 401(k) plus a company match Please contact: Stephanie Thompson sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From relia1 at actsend.com Fri May 24 10:36:36 2024 From: relia1 at actsend.com (Pam Barker) Date: Fri, 24 May 2024 15:36:36 +0000 Subject: [Histonet] Happy Memorial Day Weekend Histopeeps!! Message-ID: <76.9C.26764.404B0566@kf.mta1vrest.cc.prd.sparkpost> It's Memorial Day Weekend Histopeeps! Happy Memorial Day Histopeeps!?? Whatever your plans are I hope you make the most of them. Let?s honor our fallen heroes while cherishing the simple joys of summer. ??? Thanks-Pam? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net? ? Click Here to Unsubscribe RELIA Solutions histonet at lists.utsouthwestern.edu 5703 Red Bug Lake Road #330
Winter Springs, fl 32708 From mtompkins at emsdiasum.com Fri May 24 18:26:37 2024 From: mtompkins at emsdiasum.com (Michael Tompkins) Date: Fri, 24 May 2024 23:26:37 +0000 Subject: [Histonet] histopathology of archeological specimens In-Reply-To: <76.9C.26764.404B0566@kf.mta1vrest.cc.prd.sparkpost> References: <76.9C.26764.404B0566@kf.mta1vrest.cc.prd.sparkpost> Message-ID: Hi team, A colleague at the University of Arizona is looking for anyone with an interest in histopathology of archeological specimens. Please contact Sharon Dial at sdial at arizona.edu if you have an interest in this area. The specimen is a coyote tibia with a possible fungal lesion suggestive of coccidioidomycosis. Michael Tompkins MS CRM | Territory Account Manager- West mtompkins at emsdiasum.com Cell: 480-622-7965 Office: 800-523-5874 Fax: 215-412-8450 http://www.emsdiasum.com/ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at actsend.com Tue May 28 12:06:53 2024 From: relia1 at actsend.com (Pam Barker) Date: Tue, 28 May 2024 17:06:53 +0000 Subject: [Histonet] RELIA Histology Careers Bulletin. READ this TOMORROW! Message-ID: Hello Histopeeps READ THIS TOMORROW!? Or today if you have time! I know how crazy a Monday can be especially THIS Monday!!??After A Holiday Weekend! I have some exciting opportunities, and most are ? RELIA EXCLUSIVES, so that?s why I am contacting YOU.? Several of these opportunities are confidential searches. If you are even considering a change Shoot Me An Email! Let Me Know WHERE and WHAT you are or might be interested in and I WILL Tell you if any of these confidential searches fit what YOU are looking for! I Have Exciting Opportunities At All Levels Of Expertise! Here Are Some Of The Locations: ???????? Florida ???????? Illinois ???????? Indiana ???????? Arizona ???????? Virginia ???????? Texas ???????? New Mexico ???????? South Dakota ???????? Georgia I also have confidential opportunities in a few OTHER places And New Opportunities coming in DAILY! These are full time PERM positions and the pay is VERY competitive! Most of my clients offer relo/sign on bonuses up to 20K! My Clients Are Paying The SAME Or MORE Than Travel Jobs! My Question Is Do You Know Of Anyone Who Might Be Interested In A New Opportunity? Or Histopeeps? Would you be interested in a new opportunity? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place.? So, If You Think You Or Someone You Know Might Be Interested- Please Contact Me! I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Click Here to Unsubscribe? RELIA Solutions? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? histonet at lists.utsouthwestern.edu From melissa at alliedsearchpartners.com Wed May 29 14:07:55 2024 From: melissa at alliedsearchpartners.com (Melissa Owens) Date: Wed, 29 May 2024 19:07:55 +0000 Subject: [Histonet] MOHS Histotech Temp Coverage Needed in Denver Message-ID: Hello- I have a need for Temp MOHS coverage in Denver, CO area. Below are dates. This would be an AM shift doing 6-8 cases with the MOHS Surgeon each day listed below. Will take someone to do 1 or all of those dates below. Please email directly if you have those dates available during the AM/Morning/Day shift hours. Must have recent MOHS experience and comfortable with showing up and completing the job with no training. July 18 August 15, 22 and 29 September 5, 12, 19 and 26 October 3, 10, 24 and 31 November 7 and 14 December 5, 12, 19 and 26 Thank you! Melissa, Allied Search Partners From c.tague at pathologyarts.com Wed May 29 14:16:14 2024 From: c.tague at pathologyarts.com (Curt Tague) Date: Wed, 29 May 2024 19:16:14 +0000 Subject: [Histonet] 100% ethanol in tissue processing Message-ID: Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt From katherine.davoli at pitt.edu Wed May 29 14:22:25 2024 From: katherine.davoli at pitt.edu (Davoli, Katherine A) Date: Wed, 29 May 2024 19:22:25 +0000 Subject: [Histonet] 100% ethanol in tissue processing In-Reply-To: References: Message-ID: Totally can be used. I use ethanol rather than isopropanol in my processor with 3 stations of 100% ethanol right before the xylenes. Katherine Davoli, BA, HTL(ASCP)cm (they/them/theirs) Supervisor & Lab Manager Tissue Culture & Histology Core U of Pitt, Dept of Ophthalmology 7th Floor, UPMC Mercy Pavilion 1622 Locust St, Pittsburgh PA 15219 (412) 624-8508 katherine.davoli at pitt.edu ________________________________ From: Curt Tague via Histonet Sent: Wednesday, May 29, 2024 3:16:14 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] 100% ethanol in tissue processing Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Ckatherine.davoli%40pitt.edu%7Cfb581a9f4476489279e408dc8013e0bd%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C638526070067152010%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=5b7IfmJ%2F2HerlDdF5QU2iaGflkkeRJ8FF3CLKzAKOIk%3D&reserved=0 From paula at excaliburpathology.com Wed May 29 14:43:59 2024 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Wed, 29 May 2024 19:43:59 +0000 (UTC) Subject: [Histonet] 100% ethanol in tissue processing In-Reply-To: References: Message-ID: <1904226131.4814640.1717011839130@mail.yahoo.com> Hi, pure ethanol is drinking alcohol (Everclear, moonshine) and is used in electron microscopy. To denature it you can add 100% isopropanol. Unless the distributer made a mistake,?I am sure you were charged a pretty penny. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Wednesday, May 29, 2024 at 02:38:26 PM CDT, Curt Tague via Histonet wrote: Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald at mtsac.edu Wed May 29 17:50:05 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 29 May 2024 22:50:05 +0000 Subject: [Histonet] 100% ethanol in tissue processing In-Reply-To: References: Message-ID: Absolutely can be used on the tissue processor or for staining. -----Original Message----- From: Curt Tague via Histonet Sent: Wednesday, May 29, 2024 12:16 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] 100% ethanol in tissue processing EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at pathologyarts.com Wed May 29 18:12:04 2024 From: c.tague at pathologyarts.com (Curt Tague) Date: Wed, 29 May 2024 23:12:04 +0000 Subject: [Histonet] 100% ethanol in tissue processing In-Reply-To: References: Message-ID: My mistake, it?s methanol?. No bueno for tissue? Curt Get Outlook for iOS ________________________________ From: Mac Donald, Jennifer Sent: Wednesday, May 29, 2024 3:50:05 PM To: Curt Tague ; Histonet at lists.utsouthwestern.edu Subject: RE: 100% ethanol in tissue processing Absolutely can be used on the tissue processor or for staining. -----Original Message----- From: Curt Tague via Histonet Sent: Wednesday, May 29, 2024 12:16 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] 100% ethanol in tissue processing EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=xxu_LXkpPKCAe9P_kzXI7q9zsKCFyArunXxppJEo5id6FvQUUNcbma3PfyW68f1O&s=in66vWsozwvAeChBgTJlM8ot_HrKxoikVr2MvJTre_I&e= From gu.lang at gmx.at Thu May 30 01:45:11 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 30 May 2024 08:45:11 +0200 Subject: [Histonet] =?utf-8?q?=C2=A0Re=3A__100=25_ethanol_in_tissue_proces?= =?utf-8?q?sing?= In-Reply-To: References: Message-ID: If there is a cytology lab near you, they will take use of it. -- Diese Nachricht wurde von meinem Android Mobiltelefon mit GMX Mail gesendet. Am 30.05.24, 01:21 schrieb Curt Tague via Histonet : My mistake, it?s methanol?. No bueno for tissue? Curt Get Outlook for iOS<[1]https://aka.ms/o0ukef> ________________________________ From: Mac Donald, Jennifer Sent: Wednesday, May 29, 2024 3:50:05 PM To: Curt Tague ; Histonet at lists.utsouthwestern.edu Subject: RE: 100% ethanol in tissue processing Absolutely can be used on the tissue processor or for staining. -----Original Message----- From: Curt Tague via Histonet Sent: Wednesday, May 29, 2024 12:16 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] 100% ethanol in tissue processing EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hi all, stupid question (despite what all the college professors said, yes, I think there are some stupid questions and this is one of them, I should know)! Someone dropped off a bunch of pure ethanol, they don't need it, I said I'd help dispose it... can't be used for processing can it? I seem to recall damaging some tissue many years ago when using ethanol... your thoughts? Maybe just clean cycles on the processors... Thanks, Curt _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu [2]https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouth western.edu_mailman_listinfo_histonet&d=DwIFAg&c=euGZstcaTDllvimEN8b 7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7Y EQ&m=xxu_LXkpPKCAe9P_kzXI7q9zsKCFyArunXxppJEo5id6FvQUUNcbma3PfyW68f1 O&s=in66vWsozwvAeChBgTJlM8ot_HrKxoikVr2MvJTre_I&e= _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu [3]http://lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. https://aka.ms/o0ukef 2. https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwIFAg&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=xxu_LXkpPKCAe9P_kzXI7q9zsKCFyArunXxppJEo5id6FvQUUNcbma3PfyW68f1O&s=in66vWsozwvAeChBgTJlM8ot_HrKxoikVr2MvJTre_I&e= 3. http://lists.utsouthwestern.edu/mailman/listinfo/histonet