[Histonet] Plastic embedding and cutting training (Charles Riley)
Carrie Disbrow
cdisbrow at msn.com
Fri Mar 29 10:36:40 CDT 2024
Hi Charles,
I found this. There may be more on Google Scholar using a more specific search.
Plastic Embedding and Sectioning of Xenopus laevis Embryos<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
YouTube<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
* Educational courses<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
14 minutes, 40 seconds<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
Apr 18, 2022<https://www.youtube.com/watch?v=ueIxTHaUkRQ>
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Today's Topics:
1. Plastic embedding and cutting training (Charles Riley)
2. certified/uncertified (Nancy Schmitt)
3. Re: certified/uncertified (Whitaker, Bonnie)
4. Reagent alcohols and tissue processors (Paula Sicurello)
5. Re: [EXT] Reagent alcohols and tissue processors (Kara, Phillip)
6. Re: [EXT] Reagent alcohols and tissue processors
(Paula Sicurello)
7. ThrowBack Thursday - Throwback to the last time you updated
your resume! Day 4 of RELIA Resume Tune-UP Week!
(relia1 at earthlink.net)
8. Re: [EXT] Reagent alcohols and tissue processors (Colleen Forster)
----------------------------------------------------------------------
Message: 1
Date: Wed, 27 Mar 2024 14:35:06 -0400
From: Charles Riley <criley at udel.edu>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Plastic embedding and cutting training
Message-ID:
<CAHMJNCg1bb4ps6HinpHqCToP=ojxv-4w081axcJaK+Zmt4YBrQ at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Hello all,
I am a research histologist at the University of Delaware. My core is
looking to bring on plastic embedding and microtomy within the next year.
I am trying to find an online training course to learn the process and or
if anyone along the East Coast near Delaware would be willing to let me
come tour your facility and shadow you sometime that would be great.
So any assistance or suggestions would be greatly appreciated.
------------------------------
Message: 2
Date: Wed, 27 Mar 2024 19:57:42 +0000
From: Nancy Schmitt <Nancy.Schmitt at mercyhealth.com>
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] certified/uncertified
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Hello-
Looking for current practices with hiring:
if you were to hire a person with BS that has already been trained in histology but is not certified -
would they be hired at same rate as certified tech?
would they be expected to get certified?
It's a slippery slope.......
Thanks
Nancy Schmitt HT, MLT(ASCP)
Confidentiality Notice:
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Message: 3
Date: Wed, 27 Mar 2024 20:09:33 +0000
From: "Whitaker, Bonnie" <Bonnie.Whitaker at osumc.edu>
To: Nancy Schmitt <Nancy.Schmitt at mercyhealth.com>, Histonet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] certified/uncertified
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Hi Nancy,
If someone is certification eligible, we hire them into the same position (for us it's a C2 level Histotechnologist) and they have one year to get certified. The pay would be the bottom off the range, with a raise when they obtain their certification. If they don't have the requisite experience, we hire them in as a C1.
Bonnie Whitaker
Ohio State University
AP Operations Director
614-293-8418
-----Original Message-----
From: Nancy Schmitt via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, March 27, 2024 3:58 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] certified/uncertified
Hello-
Looking for current practices with hiring:
if you were to hire a person with BS that has already been trained in histology but is not certified - would they be hired at same rate as certified tech?
would they be expected to get certified?
It's a slippery slope.......
Thanks
Nancy Schmitt HT, MLT(ASCP)
Confidentiality Notice:
This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
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------------------------------
Message: 4
Date: Thu, 28 Mar 2024 13:04:33 +0000 (UTC)
From: Paula Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Reagent alcohols and tissue processors
Message-ID: <472887967.2172123.1711631073040 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%.
Thanks for your insight,
Paula
Yahoo Mail: Search, Organize, Conquer
------------------------------
Message: 5
Date: Thu, 28 Mar 2024 13:14:13 +0000
From: "Kara, Phillip" <Phillip.Kara at unthsc.edu>
To: HistoNet <histonet at lists.utsouthwestern.edu>, Paula Sicurello
<patpxs at gmail.com>
Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors
Message-ID:
<SN6PR01MB3949BDF29031CE73D5FB69FCE83B2 at SN6PR01MB3949.prod.exchangelabs.com>
Content-Type: text/plain; charset="us-ascii"
So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.
Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.
Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax?
Phillip Kara, HTL | Senior Research Associate
University of North Texas Health Science Center
Division of Research and Innovation
a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
p: 918-281-9060
w: www.unthsc.edu/corelabs<http://www.unthsc.edu/corelabs><http://www.unthsc.edu/corelabs<http://www.unthsc.edu/corelabs>>
________________________________
From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, March 28, 2024 8:04 AM
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [EXT] [Histonet] Reagent alcohols and tissue processors
Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70% but I favor a lower %. One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%.
Thanks for your insight,
Paula
Yahoo Mail: Search, Organize, Conquer
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------------------------------
Message: 6
Date: Thu, 28 Mar 2024 14:14:50 +0000 (UTC)
From: Paula Sicurello <patpxs at gmail.com>
To: Phillip.Kara at unthsc.edu, HistoNet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors
Message-ID: <544715091.2194403.1711635290080 at mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
Hi Kara,
I do like the VIPs.? ?Besides getting rid of the NBF, the alcohols are getting rid of the water as well.
I learned EM first, so my processing thoughts are probably biased by that.
Paula?
Yahoo Mail: Search, Organize, Conquer
On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip<Phillip.Kara at unthsc.edu> wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax?
Phillip Kara, HTL | Senior Research Associate
University of North Texas Health Science Center
Division of Research and Innovation?
a:?3500 Camp Bowie Blvd, Fort Worth, TX 76107
p:?918-281-9060
w:www.unthsc.edu/corelabs
From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, March 28, 2024 8:04 AM
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [EXT] [Histonet] Reagent alcohols and tissue processors?Good Morning,
My question two part:
Best tissue processor ever and why?
What is percentage is your first alcohol step?
I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%.
Thanks for your insight,
Paula
Yahoo Mail: Search, Organize, Conquer
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------------------------------
Message: 7
Date: Thu, 28 Mar 2024 12:18:36 -0400
From: <relia1 at earthlink.net>
To: <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] ThrowBack Thursday - Throwback to the last time
you updated your resume! Day 4 of RELIA Resume Tune-UP Week!
Message-ID: <01ee01da812b$974f1490$c5ed3db0$@earthlink.net>
Content-Type: text/plain; charset="us-ascii"
Hi Histopeeps! Happy Thursday!
Let's Throwback Thursday to the last time you updated your resume!
Yep It's Day 4 of RELIA Resume Tune-up WEEK!
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It's Free and It's simple.
All you have to do is send me an email to relia1 at earthlink.net
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with the subject line: "Im in" !
Histopeeps, Let me help you get your resume tuned up!!
It's Free of charge to my histopeeps!
#ThrowbackThursday
#Thursday
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#histologyjobs
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Thanks-Pam
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Phone: (407)657-2027
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Follow my hashtags to make your day great and your career greater!
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------------------------------
Message: 8
Date: Thu, 28 Mar 2024 11:55:04 -0500
From: Colleen Forster <cforster at umn.edu>
To: Paula Sicurello <patpxs at gmail.com>
Cc: Phillip.Kara at unthsc.edu, HistoNet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors
Message-ID:
<CAGW4+=qpe-V9sdjanFBGptyW7g46F=LMLnqP3uprHUTz7ZHByA at mail.gmail.com>
Content-Type: text/plain; charset="UTF-8"
Paula,
I start my processor at 80%. I am in research and often the samples have
been fixed and are put into 70-80% ethanol for holding or transport.
Starting with 80% steps right in line with the samples. I rarely see a
schedule that starts at 50%. However, I do have a couple labs that ask me
for that. I am fortunate to have a second processor I can use for special
programs and not hold up my main runs.
Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS Histology and IHC Laboratory
On Thu, Mar 28, 2024 at 9:15?AM Paula Sicurello via Histonet <
histonet at lists.utsouthwestern.edu> wrote:
> Hi Kara,
> I do like the VIPs. Besides getting rid of the NBF, the alcohols are
> getting rid of the water as well.
> I learned EM first, so my processing thoughts are probably biased by that.
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
>
> On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip<Phillip.Kara at unthsc.edu>
> wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to
> use, easy maintenance, and very customizable for programed runs.Every lab I
> have been in has always started at 70. It saves the techs extra time mixing
> up dilutions because you can always find RTU 70%. It also helps I have
> never had any issues with the tissues starting at 70. I get the idea of
> starting lower but you are also then going to need to increase your
> processing time which can delay turnaround times.Plus correct me if I am
> wrong but isn't the whole point of the alcohols after NBF to get the NBF
> out of the tissue and ready for xylene and wax?
>
> Phillip Kara, HTL | Senior Research Associate
>
> University of North Texas Health Science Center
>
> Division of Research and Innovation
>
> a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
>
> p: 918-281-9060
>
> w:www.unthsc.edu/corelabs
>
> From: Paula Sicurello via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Thursday, March 28, 2024 8:04 AM
> To: HistoNet <histonet at lists.utsouthwestern.edu>
> Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good
> Morning,
> My question two part:
> Best tissue processor ever and why?
> What is percentage is your first alcohol step?
> I see that lots of places start at 70% but I favor a lower %. One
> because it's gentler on the tissue, and two because 10% NBF precipitates
> out if you start at 70%.
> Thanks for your insight,
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
>
> https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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