From fpearsa at clemson.edu Fri Mar 1 06:53:08 2024 From: fpearsa at clemson.edu (Frances Pearsall) Date: Fri, 1 Mar 2024 12:53:08 +0000 Subject: [Histonet] Epredia Revos Tissue Processor Message-ID: Hi histo-peeps, I would like to know any one's experience with the Epredia Revos Tissue Processors especially animal related tissues? Thank you, Fran Pearsall From nmargaryan88 at gmail.com Fri Mar 1 09:56:28 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Fri, 1 Mar 2024 09:56:28 -0600 Subject: [Histonet] Epredia Revos Tissue Processor In-Reply-To: References: Message-ID: Hi, I would like to learn as well! Thanks, Naira On Fri, Mar 1, 2024 at 6:59?AM Frances Pearsall via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi histo-peeps, I would like to know any one's experience with the Epredia > Revos Tissue Processors especially animal related tissues? > > Thank you, Fran Pearsall > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From chak_bou at yahoo.com Sun Mar 3 13:03:38 2024 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Sun, 3 Mar 2024 19:03:38 +0000 (UTC) Subject: [Histonet] IHC References: <228177893.2002535.1709492618831.ref@mail.yahoo.com> Message-ID: <228177893.2002535.1709492618831@mail.yahoo.com> Greetings Histonet community, I'm interested in learning if anyone has experience withperforming Von Willebrand factor and alpha smooth muscle actin IHC staining onpig tissues (FFPE). If you do, could you please share the protocol along withdetails about the antibodies used, including ordering cat# and clones? Any assistance you could provide would be immensely appreciated. Best regards, Chakib From relia1 at earthlink.net Mon Mar 4 11:58:49 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 4 Mar 2024 12:58:49 -0500 Subject: [Histonet] ICYMI: 7 Tips for Effective Multi-Generational Leadership Message-ID: <00bb01da6e5d$9cede060$d6c9a120$@earthlink.net> Hello Histonetters, I hope this is the beginning of a productive and successful week for you! I found this great read and wanted to share it with you. 7 Tips ICYMI: 7 Tips for Effective Multi-generational Team Leadership. https://www.themuse.com/advice/tips-for-effective-multigenerational-leadersh ip Histonetters!! If you have any immediate or future needs, please let me know. I am happy to help. I am connected to some of the best techs in the nation and they are NOT job hunting! I can be reached on my cell at 407-353-5070 or toll free at 866-607-3542. Or via email at relia1 at earthlink.net If you would like information on how our services work, I would be happy to send it to you. Have A Great Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at actsend.com Tue Mar 5 11:43:37 2024 From: relia1 at actsend.com (Pam Barker) Date: Tue, 05 Mar 2024 17:43:37 +0000 Subject: [Histonet] It's Tip Tuesday! Need Info on Spring Histology Meetings or Histotechnology Professionals Day? I can help! Message-ID: <92.91.28296.9C957E56@il.mta1vrest.cc.prd.sparkpost> Hi?Histopeeps??! It's Tip Tuesday! And I have a couple for you! Interested in attending a state histology society meeting? v Check your state's website or social media page! v Check in our groups on Facebook and LinkedIn! v All of the info is there! v There are some AMAZING meetings being held this Spring! v It's a fantastic way to connect to your community. v Learn and earn CEUs! And It's FUN!!!! If you need info for your local meeting contact me ASAP I will get it to you! Histopeeps!?Histotechnology Professionals Day is March 10th and that is less than a week away! What are you doing to mark the occasion? Need some ideas? Email me at relia1 at earthlink.net and I will send you some great info on how to celebrate YOUR DAY !! If you are looking for a new opportunity, I can help with that too! If you are looking for a fantastic addition to your team, I can help with that too!! If you need a resume tune-up, I can help with that too! Email me relia1 at earthlink.net Call/text me at 407-353-5070 Have a Fantastic Day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Click Here to Unsubscribe? RELIA Solutions? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? histonet at lists.utsouthwestern.edu From nmargaryan88 at gmail.com Tue Mar 5 12:27:34 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Tue, 5 Mar 2024 12:27:34 -0600 Subject: [Histonet] Modified Davidson solution Message-ID: Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira From katherine.davoli at pitt.edu Tue Mar 5 15:40:17 2024 From: katherine.davoli at pitt.edu (Davoli, Katherine A) Date: Tue, 5 Mar 2024 21:40:17 +0000 Subject: [Histonet] Modified Davidson solution In-Reply-To: References: Message-ID: Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts ________________________________ From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Ckatherine.davoli%40pitt.edu%7C2b8c6695cc2d4e30566908dc3d4201ed%7C9ef9f489e0a04eeb87cc3a526112fd0d%7C1%7C0%7C638452600931337419%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=h5lm3d9fxa3KMnkU7kfLsnULNGoYzsc3BQVtiPtVLVM%3D&reserved=0 From jkiernan at uwo.ca Wed Mar 6 01:32:52 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 6 Mar 2024 07:32:52 +0000 Subject: [Histonet] Modified Davidson solution In-Reply-To: References: Message-ID: The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture acidified with acetic acid. Like all others of that ilk it was intended for making up in the lab soon before using. Storage causes slow deterioration. The ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which has no fixative properties. This change is evident from the fruity smell of the ester, which becomes evident after a week or two. All this has been in the textbooks for more than 50 years. Why doesn't every lab spend perhaps $200 on a couple of books? For obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the publisher, which is $30 less than Amazon's price), but there are other histotechnology books that are very good. A book may cost much less than a big bottle of a deteriorating mixture of questionable value, and you and your staff could learn a lot by reading. John Kiernan = = = ________________________________ From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts ________________________________ From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts ________________________________ From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afhenwood at outlook.com Wed Mar 6 05:25:59 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Wed, 6 Mar 2024 11:25:59 +0000 Subject: [Histonet] Modified Davidson solution In-Reply-To: References: Message-ID: I agree. A great book. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: John Kiernan via Histonet Sent: 06 March 2024 18:32 To: Histonet ; Naira Margaryan ; Davoli, Katherine A Subject: Re: [Histonet] Modified Davidson solution The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture acidified with acetic acid. Like all others of that ilk it was intended for making up in the lab soon before using. Storage causes slow deterioration. The ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which has no fixative properties. This change is evident from the fruity smell of the ester, which becomes evident after a week or two. All this has been in the textbooks for more than 50 years. Why doesn't every lab spend perhaps $200 on a couple of books? For obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the publisher, which is $30 less than Amazon's price), but there are other histotechnology books that are very good. A book may cost much less than a big bottle of a deteriorating mixture of questionable value, and you and your staff could learn a lot by reading. John Kiernan = = = ________________________________ From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts ________________________________ From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Davoli, Katherine A via Histonet Sent: March 5, 2024 4:40 PM To: Histonet ; Naira Margaryan Subject: Re: [Histonet] Modified Davidson solution Hi Naira, I get Hartmann's from Electron Microscopy Sciences Cat# 64133-10 (for 1L, but they have larger sizes I believe) Katherine Davoli, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture and Histology Core Facilities U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts ________________________________ From: Naira Margaryan via Histonet Sent: Tuesday, March 5, 2024 1:27 PM To: Histonet Subject: [Histonet] Modified Davidson solution Hello! What is the best company to buy a ready to use Modified Davidson solution? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Mar 6 10:25:58 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 6 Mar 2024 11:25:58 -0500 Subject: [Histonet] RELIA HOT Job Alert!! An Exciting Management Opportunity!! Message-ID: <04f901da6fe2$f9a98760$ecfc9620$@earthlink.net> Hi Histonetters! I hope you're having a fantastic day. Just a quick email about this amazing opportunity! Why do I think it is amazing? This is the perfect opportunity to STEP UP! This is a role for: * An experienced Histology Manager * An experienced Histology Supervisor ready for more! * An experienced Histology Tech ready to move UP! * This is a Stellar Management Opportunity! Manager, Supervisor or Lead Tech - This is your chance to GROW! Do you long for Small Town Life? How about outdoorsy living? I am working with a lab located in Rapid City, SD that is in need of a Histology Supervisor. They are in need of an experienced histology Manager, Supervisor or Lead Tech ready for the next level, who has strong clinical and leadership skills. They have a team of histotechs eager to be lead!! My client offers an excellent salary, benefits, and relocation assistance. If this is the right job for you RELIA can make it happen! For more information, please contact Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542 or on my cell at 407-353-5070. I also have new opportunities coming in daily nationwide so if you are interested in another area or role, please contact me! ICYMI. If your team is shorthanded, You can earn a referral reward by connecting me with the hiring authority. Contact me and I will fill you in on the details! Have a great day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From cforster at umn.edu Wed Mar 6 15:36:10 2024 From: cforster at umn.edu (Colleen Forster) Date: Wed, 6 Mar 2024 15:36:10 -0600 Subject: [Histonet] Modified Davidson solution In-Reply-To: References: Message-ID: I agree. Making some of these simple solutions that need to be FRESH is the better way to go. As John said, there are ma ny books with these formulas in them. I have used Davidson's for different projects and I make mine in the lab fresh each time tissue is harvested. Colleen Forster HT(ASCP)QIHC On Wed, Mar 6, 2024 at 5:26?AM Tony Henwood via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I agree. > > A great book. > > > Regards, > > > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > > Principal Scientist, the Children?s Hospital at Westmead (Retired) > > Adjunct Fellow, School of Medicine, University of Western Sydney. > > ________________________________ > From: John Kiernan via Histonet > Sent: 06 March 2024 18:32 > To: Histonet ; Naira Margaryan < > nmargaryan88 at gmail.com>; Davoli, Katherine A > Subject: Re: [Histonet] Modified Davidson solution > > The Davidson/Hartmann fixative is just another alcoholic formaldehyde > mixture acidified with acetic acid. Like all others of that ilk it was > intended for making up in the lab soon before using. Storage causes slow > deterioration. The ethanol slowly gets esterified by the acetic acid, > making ethyl acetate, which has no fixative properties. This change is > evident from the fruity smell of the ester, which becomes evident after a > week or two. All this has been in the textbooks for more than 50 years. > Why doesn't every lab spend perhaps $200 on a couple of books? For > obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from > the publisher, which is $30 less than Amazon's price), but there are other > histotechnology books that are very good. A book may cost much less than a > big bottle of a deteriorating mixture of questionable value, and you and > your staff could learn a lot by reading. > John Kiernan > = = = > ________________________________ > From: Davoli, Katherine A via Histonet > Sent: March 5, 2024 4:40 PM > To: Histonet ; Naira Margaryan < > nmargaryan88 at gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm (they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > ________________________________ > From: Naira Margaryan via Histonet > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ________________________________ > From: Davoli, Katherine A via Histonet > Sent: March 5, 2024 4:40 PM > To: Histonet ; Naira Margaryan < > nmargaryan88 at gmail.com> > Subject: Re: [Histonet] Modified Davidson solution > > Hi Naira, I get Hartmann's from Electron Microscopy Sciences > Cat# 64133-10 (for 1L, but they have larger sizes I believe) > > > Katherine Davoli, HTL(ASCP)cm (they/them/theirs) > > Lab Manager, Tissue Culture and Histology Core Facilities > > U. Pitt Dept of Ophthalmology > > 7.373 UPMC Mercy Pavilion > > 1622 Locust St., Pittsburgh PA 15219 > > (412) 624-8508 this number cannot receive texts > > ________________________________ > From: Naira Margaryan via Histonet > Sent: Tuesday, March 5, 2024 1:27 PM > To: Histonet > Subject: [Histonet] Modified Davidson solution > > Hello! > > What is the best company to buy a ready to use Modified Davidson solution? > > Thanks in advance, > Naira > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > http://lists.utsouthwestern.edu/mailman/listinfo/histonet> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From relia1 at earthlink.net Mon Mar 11 12:46:14 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 11 Mar 2024 13:46:14 -0400 Subject: [Histonet] It's Spring Break and timing is EVERYTHING! Histology Recruitment Opportunity! Message-ID: <001901da73dc$03e09460$0ba1bd20$@earthlink.net> Hello Histonetters! I hope you are having a great day! Can you believe it's the middle of March? Spring Break is ON! It's that time of year for trips to new and favorite places .... This time of year also presents a unique opportunity for you as an employer. Many Candidates are willing to do an interview while on their Spring Break as a matter of fact... I always hear from many histotechs this time of year who say it's time for: * A new challenge * A change of scenery * A move closer to family I may have just spoken with someone interested in your area! Histonetters! that's why I'm posting, to see if you have any current or anticipated histology openings you need assistance with. I curate a nationwide network of over 9000 histology professionals that I communicate with regularly. I am aware of who among them is looking for a new position and who is considering a move. I know why they want to make a job change, where they want to live and what their credentials are. I don't advertise on the big job boards! Why duplicate your efforts? I rely on my contacts and expertise to enhance your hiring process. If you would like assistance with an immediate need or would like to discuss working with me in the future I would be happy to contact you or you can contact me at toll free 866-607-3542 or cell 407-353-5070 or relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Wed Mar 13 11:30:44 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 13 Mar 2024 12:30:44 -0400 Subject: [Histonet] ICYMI: Spring Break is ON! Please Read ON for Some Interesting Spring Break Info! Message-ID: <001401da7563$ccbbe910$6633bb30$@earthlink.net> Hi Histopeeps! Happy Hump Daaaaay! ICYMI Spring Break is ON! Did I see you on Social Media traveling to fun and exotic places? I hope you are having a BLAST! If you happen to visit a place you might like to live LET ME KNOW! I might have a great opportunity NOW or LATER when the timing is better for YOU! Thinking about travel histology? Think AGAIN!! Rates Are Falling And Good Contracts Are Drying Up! So Histopeeps, you might want to consider a permanent position... ?Some of my positions pay even MORE than travel contracts! ?My Clients are the CREAM of the CROP! The best histology labs in the U.S. ?Some of my clients are offering sign on bonuses up to 20K ?All of my clients offer EXCELLENT compensation packages including relocation! Here is some Info on my Current Opportunities. I have opportunities in: ? Education ? Leadership ? IHC ? Derm ? GI ? Mohs ? DX I have opportunities located in: ? Virginia ? Florida ? Georgia ? Alabama ? South Dakota ? California I also have new opportunities coming in daily nationwide so if you are interested in another area or role, please contact me! ICYMI? If your team is shorthanded, You can earn a referral reward by connecting me with the hiring authority. Contact me and I will fill you in on the details! You can reach me at relia1 at earthlink.net OR Cell/text 407-353-5070 p.s. I invite you to network with your fellow histopeeps in my facebook group www.facebook.com/groups/histotechnologists Have a great day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From andrea at ka-recruiting.com Wed Mar 13 12:04:14 2024 From: andrea at ka-recruiting.com (Andrea Costello) Date: Wed, 13 Mar 2024 13:04:14 -0400 Subject: [Histonet] You Don't Want to Miss Out on These Histology Openings In-Reply-To: References: Message-ID: Hello Histonet! I just wanted to send you a quick email updating you on all of our active histology positions! As a reminder, all of our positions are *permanent. *As such, they all offer highly competitive hourly rates, opportunities for growth and advancement and comprehensive benefits packages! Benefits include (but are not limited to): - Healthcare, Dental and Pharmacy Benefits - 403(B) and 401(K) Options - Generous PTO and Holiday Time Off - Tuition Reimbursement - Sign On Bonus and/or Relocation Assistance Our Current Openings: - Florida - Northern: Histotech - Derm Grossing Experience Required - Florida - Northern: Histotech - MOHS Experience Preferred - Georgia - Eastern: Histotech - Second or Third Shift - Illinois - Greater Chicago - Histotech - Second or Third Shift - Illinois - Greater Chicago: IHC Specialist- Second or Third Shift - Missouri - Greater Kansas City: Histology Supervisor - North Carolina - Western: Histotech - Nights - New Hampshire - Central: Histotech - Evening or Nights - Nevada - Western - Histotech/ Grossing Position - Late Days - New York - Central New York - Histotech - Days - New York - Finger Lakes - Histotech - Days - New York - Hudson Valley - Histology Manager - New York - Long Island - Histotech - Full or Part Time - Multiple Shifts Available! - New York - Westchester - Grossing Technician - Days or Nights - South Carolina - Western - Histotech - Evening Shift - Virginia - Coastal - Histotechnician - Evenings or Nights - Virginia - Coastal - Senior Histology Educator - Days Looking forward to connecting with you soon! Andrea Costello K.A. Recruiting, Inc. 10 Post Office Square 8th Floor SOUTH Boston, MA 02109 *P: (617) 746-2745* *F: (617) 507-8009* *E: andrea at ka-recruiting.com * Link to my calendar: https://calendly.com/andreaka/15min On Wed, Mar 13, 2024 at 1:00?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. ICYMI: Spring Break is ON! Please Read ON for Some > Interesting Spring Break Info! (relia1 at earthlink.net) > > > > ---------- Forwarded message ---------- > From: > To: > Cc: > Bcc: > Date: Wed, 13 Mar 2024 12:30:44 -0400 > Subject: [Histonet] ICYMI: Spring Break is ON! Please Read ON for Some > Interesting Spring Break Info! > Hi Histopeeps! > > Happy Hump Daaaaay! > > ICYMI Spring Break is ON! > > Did I see you on Social Media traveling to fun and exotic places? > > I hope you are having a BLAST! > > If you happen to visit a place you might like to live > > LET ME KNOW! > > I might have a great opportunity NOW > > or > > LATER when the timing is better for YOU! > > Thinking about travel histology? > > Think AGAIN!! > > Rates Are Falling And Good Contracts Are Drying Up! > > So Histopeeps, you might want to consider a permanent position... > > ?Some of my positions pay even MORE than travel contracts! > > ?My Clients are the CREAM of the CROP! > > The best histology labs in the U.S. > > ?Some of my clients are offering sign on bonuses up to 20K > > ?All of my clients offer EXCELLENT compensation packages including > relocation! > > Here is some Info on my Current Opportunities. > > I have opportunities in: > > ? Education > > ? Leadership > > ? IHC > > ? Derm > > ? GI > > ? Mohs > > ? DX > > I have opportunities located in: > > ? Virginia > > ? Florida > > ? Georgia > > ? Alabama > > ? South Dakota > > ? California > > I also have new opportunities coming in daily nationwide so if you are > interested in another area or role, please contact me! > > ICYMI? If your team is shorthanded, You can earn a referral reward by > connecting me with the hiring authority. Contact me and I will fill you in > on the details! > > You can reach me at relia1 at earthlink.net > > OR > > Cell/text 407-353-5070 > > p.s. I invite you to network with your fellow histopeeps in my facebook > group www.facebook.com/groups/histotechnologists > > > Have a great day! > > Thanks-Pam > > > > > > Right Time, Right Place, Right Move with RELIA! > > Providing excellent service exclusively to the Histology Community! > > > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5717 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > > Toll free: (866)60RELIA or (866)607-3542 > E-mail: relia1 at earthlink.net > > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > > > www.linkedin.com/in/reliasolutions > > Follow my hashtags to make your day great and your career greater! > > #ilovemyhistopeeps > > #jobs4myhistopeeps > > #histologyiscool > > #histologyjobs > > #histologycareers > > #histology > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From modz9636 at gmail.com Wed Mar 13 12:37:40 2024 From: modz9636 at gmail.com (M.O.) Date: Wed, 13 Mar 2024 10:37:40 -0700 Subject: [Histonet] vasculature staining in 3D Message-ID: Happy Wednesday! I have an image in my head and I cannot find any image that matches what I saw - cleared 3D tissue, maybe it was lungs or a heart with blue vessels/veins stained. I've done skeletal staining on mouse embryos with alcian blue and alizarin red and was wondering if there is something similar for soft tissue and vessels (not fluorescent). Is this something that exists? I've seen vascular casting, but couldn't find images of cleared tissue with stained vessels. Thank you for any hints to my mystery! -Merissa From paula at excaliburpathology.com Wed Mar 13 12:53:35 2024 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Wed, 13 Mar 2024 17:53:35 +0000 (UTC) Subject: [Histonet] vasculature staining in 3D In-Reply-To: References: Message-ID: <137038063.3065406.1710352415971@mail.yahoo.com> Hi, I have done retinal flat mounts to visualize vasculature with lectins and used chromagenic instead of fluoro. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Wednesday, March 13, 2024 at 12:44:36 PM CDT, M.O. via Histonet wrote: Happy Wednesday! I have an image in my head and I cannot find any image that matches what I saw - cleared 3D tissue, maybe it was lungs or a heart with blue vessels/veins stained. I've done skeletal staining on mouse embryos with alcian blue and alizarin red and was wondering if there is something similar for soft tissue and vessels (not fluorescent). Is this something that exists? I've seen vascular casting, but couldn't find images of cleared tissue with stained vessels. Thank you for any hints to my mystery! -Merissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mashalsmom at gmail.com Wed Mar 13 14:19:28 2024 From: mashalsmom at gmail.com (Emily Horst) Date: Wed, 13 Mar 2024 15:19:28 -0400 Subject: [Histonet] C4d Message-ID: Hello, Does anyone in here run C4d (SP91) on the Benchmark Ultras and would be willing to share your protocol? I am having a really hard time getting this stain to where the pathologists want it. Thank you Emily Horst Histology Supervisor University of Cincinnati Medical Center From Diana.Martinez-Longoria at ecrmc.org Thu Mar 14 07:58:30 2024 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 14 Mar 2024 12:58:30 +0000 Subject: [Histonet] Tissue Processor Down Question Message-ID: <31d176fcd762472294877ebba56d8ef3@ecrmc.org> Good morning, Our VIP5 tissue processor went down and our tissue cassettes were in clearing solution. What is the best media to put them while we wait for the tissue processor to be repaired, please let me know? Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. From Karen.Schieberl at commonspirit.org Thu Mar 14 08:39:39 2024 From: Karen.Schieberl at commonspirit.org (Karen Schieberl CA-San Francisco) Date: Thu, 14 Mar 2024 06:39:39 -0700 Subject: [Histonet] MMR Message-ID: Good Morning, I am looking for literature on minimum and maximum formalin time for breast tissue for MMR testing. I know about this time for ER/PR, HER2 and fish testing. I need the literature for the MMR fixation time on Breast tissue. I thought that you do not even do MMR on breast tissue. Your help is greatly appreciated. Thanks, Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From gu.lang at gmx.at Thu Mar 14 09:28:26 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 14 Mar 2024 15:28:26 +0100 Subject: [Histonet] Tissue Processor Down Question In-Reply-To: <31d176fcd762472294877ebba56d8ef3@ecrmc.org> References: <31d176fcd762472294877ebba56d8ef3@ecrmc.org> Message-ID: <000401da761b$e162bcf0$a42836d0$@gmx.at> Depending on the time the cassettes were in clearing medium, I would transfer them into molten paraffin at least for double of the time in the processor. And then embed etc. Try a few blocks to see, if infiltration was sufficient. If cutting is hampered, prolong the time in paraffin. Gudrun -----Urspr?ngliche Nachricht----- Von: Diana Martinez-Longoria via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 14. M?rz 2024 13:59 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Tissue Processor Down Question Wichtigkeit: Hoch Good morning, Our VIP5 tissue processor went down and our tissue cassettes were in clearing solution. What is the best media to put them while we wait for the tissue processor to be repaired, please let me know? Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diana.Martinez-Longoria at ecrmc.org Thu Mar 14 09:49:15 2024 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 14 Mar 2024 14:49:15 +0000 Subject: [Histonet] Tissue Processor Down Question In-Reply-To: <000401da761b$e162bcf0$a42836d0$@gmx.at> References: <31d176fcd762472294877ebba56d8ef3@ecrmc.org> <000401da761b$e162bcf0$a42836d0$@gmx.at> Message-ID: <26657c5b265a4a6b9146722968fa8635@ecrmc.org> Okay, thank you for your advice. Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA? 92243 760.339.7267: Fax: 760-3394570 ?Diana.Martinez-Longoria at ecrmc.org -----Original Message----- From: Gudrun Lang [mailto:gu.lang at gmx.at] Sent: Thursday, March 14, 2024 7:28 AM To: Diana Martinez-Longoria Cc: histonet at lists.utsouthwestern.edu Subject: AW: [Histonet] Tissue Processor Down Question Warning! This email was sent from outside your organization. Do not click or open attachments if you don't recognize the sender. Depending on the time the cassettes were in clearing medium, I would transfer them into molten paraffin at least for double of the time in the processor. And then embed etc. Try a few blocks to see, if infiltration was sufficient. If cutting is hampered, prolong the time in paraffin. Gudrun -----Urspr?ngliche Nachricht----- Von: Diana Martinez-Longoria via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 14. M?rz 2024 13:59 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Tissue Processor Down Question Wichtigkeit: Hoch Good morning, Our VIP5 tissue processor went down and our tissue cassettes were in clearing solution. What is the best media to put them while we wait for the tissue processor to be repaired, please let me know? Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. From tabbott at pennstatehealth.psu.edu Thu Mar 14 12:49:41 2024 From: tabbott at pennstatehealth.psu.edu (Abbott, Tanya) Date: Thu, 14 Mar 2024 17:49:41 +0000 Subject: [Histonet] Teaching Message-ID: I am looking for any information anyone can offer regarding using/teaching Histology techniques in a high school setting. Any input is greatly appreciated! Feel free to email me, thanks! Tanya Abbott arhs85 at yahoo.com From vtolley25 at gmail.com Fri Mar 15 09:42:31 2024 From: vtolley25 at gmail.com (Val T) Date: Fri, 15 Mar 2024 07:42:31 -0700 Subject: [Histonet] MMR In-Reply-To: <84DFA3B0-3B52-4919-8345-6272FE2CF02D@gmail.com> References: <84DFA3B0-3B52-4919-8345-6272FE2CF02D@gmail.com> Message-ID: <8162866B-7C55-4E26-89C1-3AD9A7BB702A@gmail.com> > Hey Karen > > CAP doesn?t have a guideline for MMR (yet), like they do with breast markers. However, there are some publications and antibody vendor inserts that address how formalin time affects MMR protein expression. (See links below). > Ventana assay recommends 6-48hr window. > > MMR panel is really starting to branch outside the colon and endometrial cancer umbrella. It is being used on a lot of different tumor types (including breast) for both therapeutic and prognostic indicators. See study: "Impact of formalin fixation on mismatch repair? Also see: MMR IHC Panel Interpretation Guide from Ventana > > Val > > >> On Mar 14, 2024, at 6:49?AM, Karen Schieberl CA-San Francisco via Histonet wrote: >> >> ?Good Morning, >> I am looking for literature on minimum and maximum formalin time for breast >> tissue for MMR testing. I know about this time for ER/PR, HER2 and fish >> testing. I need the literature for the MMR fixation time on Breast >> tissue. >> >> I thought that you do not even do MMR on breast tissue. >> >> Your help is greatly appreciated. >> >> Thanks, >> Karen Schieberl HT ASCP >> >> Lead Histology Technician >> >> St. Mary's Medical Center >> >> 450 Stanyan St. >> >> San Francisco, Ca. 94117 >> >> 415-750-5751 >> >> karen.schieberl@ commonspirit.org >> >> >> >> >> Caution: This email message, including all content and attachments, is >> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The >> information contained in this email message is intended only for the use of >> the recipient(s) named above. If the reader of this message is not the >> intended recipient or an agent responsible for delivering it to the >> intended recipient, you have received this document in error. Any further >> review, dissemination, distribution, or copying of this message is strictly >> prohibited. If you have received this communication in error, please >> notify us immediately by reply email. Thank you >> >> Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at udel.edu Mon Mar 18 07:22:07 2024 From: criley at udel.edu (Charles Riley) Date: Mon, 18 Mar 2024 08:22:07 -0400 Subject: [Histonet] Inflammatory testing options Message-ID: Other than H&E and Giemsa stains, what can be used to show inflammation in paraffin sections? From patpxs at gmail.com Mon Mar 18 08:15:43 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Mon, 18 Mar 2024 13:15:43 +0000 (UTC) Subject: [Histonet] A Question for DOD Histology Labs References: <327576238.4560468.1710767743752.ref@mail.yahoo.com> Message-ID: <327576238.4560468.1710767743752@mail.yahoo.com> Good Morning and Happy Monday, If you work at a Department of Defense (DOD) Histology lab, can you tell me what make and model of cassette printer and slide writer you use? Do they have an ATO? We are in the market for both and want to know what y'all are using.?Plus how do you like whatever it is you are using? Thank you, Paula Yahoo Mail: Search, Organize, Conquer From igor.deyneko at gmail.com Mon Mar 18 15:43:27 2024 From: igor.deyneko at gmail.com (Igor) Date: Mon, 18 Mar 2024 16:43:27 -0400 Subject: [Histonet] Questions about education Message-ID: Greetings , fellow Histonetters, I'm reaching our for advice on education. I've worked in research histology ages ago, set up a lab and did everything from organ harvesting and grossing to processing, staining ( routine, special, IHC, and IF). Then I went the management route and have been in lab operations since. I wanted to go back and obtain ASCP certification and get either an HTL or MLS/MLT certification in order to be eligibleto work in a diagnostic CLIA-accredited laboratory. My undergrad has been in biology, my grad degrees were in operations and management. I live in Boston. Any recommendations on how to proceed, best place to get the information, the best route, training colleges/programs of where to get the education and experience that I could contact would be greatly appreciated!!! Thank you in advance for the information! Igor From relia1 at earthlink.net Tue Mar 19 11:56:32 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 19 Mar 2024 12:56:32 -0400 Subject: [Histonet] Timing is Everything! Is it Time for a Change OR ICYMI: Should I stay or Should I go? Message-ID: <000b01da7a1e$65c75220$3155f660$@earthlink.net> Hi Histonetters! I hope you are having a fantastic day! If you are someone who wants to move up in your career NOW is the perfect time to shine! Why? The staffing shortages ARE opening opportunities for YOU to Learn MORE. Do MORE. Get MORE Experience AND Opportunity to further your career! If not at your current position then it IS out theresomewhere else! I can speak from experience! My clients are giving motivated histotechs opportunities to be hired in at higher level positions based on potential not just immediate need! My clients are offering Amazing Compensation Packages including relocation and Sign On Bonuses up to 20K The Travel Market is getting weaker. Alot of competition for the few great contracts out there. Think about it for a second... Your next opportunity might be just around the corner. If you are looking for a position right now, please contact me RIGHT AWAY! Let's chat about my current positions OR about a customized search for YOU! email me: relia1 at earthlink.net call/text 407-353-5070 If you still aren't convinced we need to chat check out this article! ICYMI 20 reasons it's time to quit your job ASAP https://www.themuse.com/advice/10-signs-you-should-quit-your-job-asap If you aren't looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. To do that, send me an email:relia1 at earthlink.net me on my cell at 407-353-5070. My Clients are looking for histopeeps to fill roles in: TEACHING!! Leadership IHC Mohs Diagnostics These amazing opportunities with some of the best labs in the USA! Here are SOME of the locations: Florida California Virginia Oregon South Dakota Georgia All of these are full time permanent positions offering the same or BETTER compensation than Travel positions! Have doubts about this let me show you how! My clients are offering relocation and or sign on bonuses up to 20K!! And new opportunities coming in on a daily basis! Most of these are RELIA Exclusives! That's right you will only see them HERE! I really appreciate you taking time to read my e-mail Thanks Again! Incidentally, if you have a specific area in mind let me know so I can be on the lookout. Thanks-Pam I would like to invite you to join my group on Facebook: http://www.facebook.com/groups/histotechnologists It's a great place to learn, laugh and share with your fellow histopeeps! **Remember if you refer someone I place your will earn a referral bonus! **Remember if you refer a job opening and I fill it you will earn a referral bonus!!! Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! I hope you have a wonderful week. Thanks-Pam Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Mon Mar 25 10:37:03 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 25 Mar 2024 11:37:03 -0400 Subject: [Histonet] Check Out These Leading Staffing Indicators for Histology! Message-ID: <00c201da7eca$50933850$f1b9a8f0$@earthlink.net> Hello Histonetters, I hope this is the start to a fantastic week! I wanted to let you know about a couple of leading indicators for histology staffing. 1. The next round of contracts for travelers ends in April! Contracts are drying up and they want to go HOME! 2. I am getting lots of questions from techs like: What states have licensure reqs, or Help me with my resume. I wanted to give you a heads up because it has been so tough lately for so many labs to find histotechs and that's why I wanted to share this small window of opportunity with you. It's only March and my phone is ringing off the hook and my email is overflowing!! *Lucrative Travel Contracts are rare! *Travelers are ready to return to permanent work! *Histotechs in Permanent positions are READY TOO! What does this mean to YOU? *Techs are NOT waiting until Summer to make that move. *Some are ready ASAP *Some are ready in March, April, May, June... Please let me know if I can help with a histology position NOW or in the FUTURE. If you are curious how my services work, I can send you info. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From denise.long at uconn.edu Tue Mar 26 09:44:03 2024 From: denise.long at uconn.edu (Long, Denise M.) Date: Tue, 26 Mar 2024 14:44:03 +0000 Subject: [Histonet] Dako Autostainer Plus Message-ID: Does anyone have the computer with Dako software on it, that will run an old Dako Autostainer Plus IHC instrument? Our computer died but the instrument is still in working order. Agilent/Dako no longer supports or repairs this instrument or the computer that runs it, so they are of no assistance. Y'all are our only hope....! Denise M. Long, MS, HT, HTL (ASCP) QIHC University of Connecticut Department of Pathobiology and Veterinary Science Connecticut Veterinary Medical Diagnostic Laboratory Histology Laboratory 61 N. Eagleville Road Storrs, CT 06269-3089 (860) 424-6715 From relia1 at earthlink.net Tue Mar 26 11:35:55 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 26 Mar 2024 12:35:55 -0400 Subject: [Histonet] Your Resume is YOUR Personal BRAND. Let's Tune it UP! Message-ID: <001c01da7f9b$adbb90a0$0932b1e0$@earthlink.net> Hi Histonetters! I hope this is a great week for you! I have been getting emails and calls from your fellow histopeeps asking if I am available to help them with their resumes. The Answer is YES! * Prepping for a performance review? * Preparing for a presentation? * Transitioning between travel and perm work? * Going for a promotion? * Going Back to School? * Going to a Networking Event? * Going to give a Workshop? * Going to Run for Office? * Going to Volunteer for a Cause? Your Updated RESUME is an Investment in YOUR Personal Brand! It's Free and It's simple. All you have to do is send me an email to relia1 at earthlink.net with the subject line: "Im in" ! Histopeeps, Let me help you get your resume tuned up!! It's Free of charge to my histopeeps! Job Hunting? Here are some of my Hottest opportunities. All of these are permanent positions. Most are RELIA Exclusives All of my clients offer excellent compensation, benefits and most offer relocation/sign on bonuses!! I have some pretty exciting opportunities that I am currently working on: Take a look and let me know what you think! Is there something on this list you want to take a look at? Let me know and I will fill you in on all of the details! Is there something on this list that is "close" to what interests you? Let me know what is right and what is not and let me find the 100% right one for you! I have opportunities in: Research/Drug Discovery QA/QC Diagnostics Immunohistochemistry Education Leadership These Stellar Positions are located in: Southwest Florida Los Angeles, CA San Diego, CA Rapid City, SD Atlanta, GA Birmingham, AL Virginia Beach, VA Charlottesville, VA Opportunities that I represent are with the "Cream of the Crop" of Histology Employers! And GUESS WHAT? My clients know YOU are also the "Cream of the Crop" because I only represent the BEST and BRIGHTEST! That's YOU!! All of the Positions I Represent Are: Full time and Permanent Offer excellent compensation packages Locations throughout the U.S. TELL ME Where you want to be! I am looking forward to hearing from you!! Call/Text 407-353-5070 email: relia1 at earthlink.net Have a Fantastic Day!!! I would like to invite you to join my Facebook group: www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From jmyers1 at aol.com Tue Mar 26 14:35:34 2024 From: jmyers1 at aol.com (Jmyers1) Date: Tue, 26 Mar 2024 19:35:34 +0000 (UTC) Subject: [Histonet] Dako Autostainer Plus In-Reply-To: References: Message-ID: <1471206242.1571097.1711481734201@mail.yahoo.com> Hi Denise:I've had extensive experience with the AutoStainer line of instruments, originally manufactured by LabVision and offered by a number IHC product vendors.? If memory serves me, the most important aspect of any (desktop-style) PC that you intend to use as a replacement for the original PC, is that it has, within its 'guts' the motion control board, which interfaces with the instrument through that very broad cable that connects it to the the PC. Provided that the replacement PC's motherboard can accept that old motion control board, it seems possible that it will connect with the instrument...but the next biggest challenge is whether or not the application software can be transferred from the old PC to the new PC. I suspect that others who are familiar with this instrument will also chime in here.? Also consider contacting the folks at Affordable IHC Solutions:?https://www.affordableihcinstruments.com/Best Wishes,Joe Myers, M.S, CT/QIHC(ASCP) Automated IHC/ISH slide staining systems: current technologies and other considerations - PubMed ********************************************************* On Tuesday, March 26, 2024 at 10:55:06 AM EDT, Long, Denise M. via Histonet wrote: Does anyone have the computer with Dako software on it,? that will run an old Dako Autostainer Plus IHC instrument? Our computer died but the instrument is still in working order. Agilent/Dako no longer supports or repairs this instrument or the computer that runs it, so they are of no assistance. Y'all are our only hope....! Denise M. Long, MS, HT, HTL (ASCP) QIHC University of Connecticut Department of Pathobiology and Veterinary Science Connecticut Veterinary Medical Diagnostic Laboratory Histology Laboratory 61 N. Eagleville Road Storrs, CT? 06269-3089 (860) 424-6715 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Mar 27 11:01:20 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 27 Mar 2024 12:01:20 -0400 Subject: [Histonet] Win The DAY Wednesday! How about a free Resume Tune-UP Day # of RELIA Resume Tune-UP Week! Message-ID: <012701da8060$037e1020$0a7a3060$@earthlink.net> Hi Histopeeps! Happy Wednesday! Lets Win The Day this Wednesday! This is DAY 3 of RELIA Resume Tune up WEEK! I have already tuned up some resumes for some amazing histopeeps and am ready for more! REMEMBER! There are lots of reasons to have an updated resume! **Prepping for a performance review? **Preparing for a presentation? **Transitioning between travel and perm work? **Going for a promotion? **Going Back to School? **Going to a Networking Event? **Going to give a Workshop? **Going to Run for Office? **Going to Volunteer for a Cause? Your Updated RESUME is an Investment in YOUR Personal Brand! It's Free and It's simple. All you have to do is send me an email to relia1 at earthlink.net with the subject line: "Im in" ! Histopeeps, Let me help you get your resume tuned up!! It's Free of charge to my histopeeps! #Wednesday #HappyWednesday #WednesdayWins #humpday #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From criley at udel.edu Wed Mar 27 13:35:06 2024 From: criley at udel.edu (Charles Riley) Date: Wed, 27 Mar 2024 14:35:06 -0400 Subject: [Histonet] Plastic embedding and cutting training Message-ID: Hello all, I am a research histologist at the University of Delaware. My core is looking to bring on plastic embedding and microtomy within the next year. I am trying to find an online training course to learn the process and or if anyone along the East Coast near Delaware would be willing to let me come tour your facility and shadow you sometime that would be great. So any assistance or suggestions would be greatly appreciated. From Nancy.Schmitt at mercyhealth.com Wed Mar 27 14:57:42 2024 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Wed, 27 Mar 2024 19:57:42 +0000 Subject: [Histonet] certified/uncertified Message-ID: Hello- Looking for current practices with hiring: if you were to hire a person with BS that has already been trained in histology but is not certified - would they be hired at same rate as certified tech? would they be expected to get certified? It's a slippery slope....... Thanks Nancy Schmitt HT, MLT(ASCP) Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From Bonnie.Whitaker at osumc.edu Wed Mar 27 15:09:33 2024 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Wed, 27 Mar 2024 20:09:33 +0000 Subject: [Histonet] certified/uncertified In-Reply-To: References: Message-ID: Hi Nancy, If someone is certification eligible, we hire them into the same position (for us it's a C2 level Histotechnologist) and they have one year to get certified. The pay would be the bottom off the range, with a raise when they obtain their certification. If they don't have the requisite experience, we hire them in as a C1. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Nancy Schmitt via Histonet Sent: Wednesday, March 27, 2024 3:58 PM To: Histonet Subject: [Histonet] certified/uncertified Hello- Looking for current practices with hiring: if you were to hire a person with BS that has already been trained in histology but is not certified - would they be hired at same rate as certified tech? would they be expected to get certified? It's a slippery slope....... Thanks Nancy Schmitt HT, MLT(ASCP) Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!Dzt8CrcZjYAK_3cE_nkD-2iArUt8vOjDA9R_FhIhNCRDNQ2IS-H3HbYFOgVH4Fe4LZKh1SRYDgqvKhKlkbcTq4fUqMxHRKx-OHIySw$ From patpxs at gmail.com Thu Mar 28 08:04:33 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 28 Mar 2024 13:04:33 +0000 (UTC) Subject: [Histonet] Reagent alcohols and tissue processors References: <472887967.2172123.1711631073040.ref@mail.yahoo.com> Message-ID: <472887967.2172123.1711631073040@mail.yahoo.com> Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer From Phillip.Kara at unthsc.edu Thu Mar 28 08:14:13 2024 From: Phillip.Kara at unthsc.edu (Kara, Phillip) Date: Thu, 28 Mar 2024 13:14:13 +0000 Subject: [Histonet] [EXT] Reagent alcohols and tissue processors In-Reply-To: <472887967.2172123.1711631073040@mail.yahoo.com> References: <472887967.2172123.1711631073040.ref@mail.yahoo.com> <472887967.2172123.1711631073040@mail.yahoo.com> Message-ID: So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs. Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times. Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 p: 918-281-9060 w: www.unthsc.edu/corelabs ________________________________ From: Paula Sicurello via Histonet Sent: Thursday, March 28, 2024 8:04 AM To: HistoNet Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70% but I favor a lower %. One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 From patpxs at gmail.com Thu Mar 28 09:14:50 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 28 Mar 2024 14:14:50 +0000 (UTC) Subject: [Histonet] [EXT] Reagent alcohols and tissue processors In-Reply-To: References: <472887967.2172123.1711631073040.ref@mail.yahoo.com> <472887967.2172123.1711631073040@mail.yahoo.com> Message-ID: <544715091.2194403.1711635290080@mail.yahoo.com> Hi Kara, I do like the VIPs.? ?Besides getting rid of the NBF, the alcohols are getting rid of the water as well. I learned EM first, so my processing thoughts are probably biased by that. Paula? Yahoo Mail: Search, Organize, Conquer On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation? a:?3500 Camp Bowie Blvd, Fort Worth, TX 76107 p:?918-281-9060 w:www.unthsc.edu/corelabs From: Paula Sicurello via Histonet Sent: Thursday, March 28, 2024 8:04 AM To: HistoNet Subject: [EXT] [Histonet] Reagent alcohols and tissue processors?Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 From relia1 at earthlink.net Thu Mar 28 11:18:36 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 28 Mar 2024 12:18:36 -0400 Subject: [Histonet] ThrowBack Thursday - Throwback to the last time you updated your resume! Day 4 of RELIA Resume Tune-UP Week! Message-ID: <01ee01da812b$974f1490$c5ed3db0$@earthlink.net> Hi Histopeeps! Happy Thursday! Let's Throwback Thursday to the last time you updated your resume! Yep It's Day 4 of RELIA Resume Tune-up WEEK! I have already tuned up some resumes for some amazing histopeeps and am ready for more! REMEMBER! There are lots of reasons to have an updated resume! **Prepping for a performance review? **Preparing for a presentation? **Transitioning between travel and perm work? **Going for a promotion? **Going Back to School? **Going to a Networking Event? **Going to give a Workshop? **Going to Run for Office? **Going to Volunteer for a Cause? Your Updated RESUME is an Investment in YOUR Personal Brand! It's Free and It's simple. All you have to do is send me an email to relia1 at earthlink.net with the subject line: "Im in" ! Histopeeps, Let me help you get your resume tuned up!! It's Free of charge to my histopeeps! #ThrowbackThursday #Thursday #ThursdayThoughts #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From cforster at umn.edu Thu Mar 28 11:55:04 2024 From: cforster at umn.edu (Colleen Forster) Date: Thu, 28 Mar 2024 11:55:04 -0500 Subject: [Histonet] [EXT] Reagent alcohols and tissue processors In-Reply-To: <544715091.2194403.1711635290080@mail.yahoo.com> References: <472887967.2172123.1711631073040.ref@mail.yahoo.com> <472887967.2172123.1711631073040@mail.yahoo.com> <544715091.2194403.1711635290080@mail.yahoo.com> Message-ID: Paula, I start my processor at 80%. I am in research and often the samples have been fixed and are put into 70-80% ethanol for holding or transport. Starting with 80% steps right in line with the samples. I rarely see a schedule that starts at 50%. However, I do have a couple labs that ask me for that. I am fortunate to have a second processor I can use for special programs and not hold up my main runs. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory On Thu, Mar 28, 2024 at 9:15?AM Paula Sicurello via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Kara, > I do like the VIPs. Besides getting rid of the NBF, the alcohols are > getting rid of the water as well. > I learned EM first, so my processing thoughts are probably biased by that. > Paula > > Yahoo Mail: Search, Organize, Conquer > > On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip > wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to > use, easy maintenance, and very customizable for programed runs.Every lab I > have been in has always started at 70. It saves the techs extra time mixing > up dilutions because you can always find RTU 70%. It also helps I have > never had any issues with the tissues starting at 70. I get the idea of > starting lower but you are also then going to need to increase your > processing time which can delay turnaround times.Plus correct me if I am > wrong but isn't the whole point of the alcohols after NBF to get the NBF > out of the tissue and ready for xylene and wax? > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w:www.unthsc.edu/corelabs > > From: Paula Sicurello via Histonet > Sent: Thursday, March 28, 2024 8:04 AM > To: HistoNet > Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good > Morning, > My question two part: > Best tissue processor ever and why? > What is percentage is your first alcohol step? > I see that lots of places start at 70% but I favor a lower %. One > because it's gentler on the tissue, and two because 10% NBF precipitates > out if you start at 70%. > Thanks for your insight, > Paula > > Yahoo Mail: Search, Organize, Conquer > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From cdisbrow at msn.com Fri Mar 29 10:36:40 2024 From: cdisbrow at msn.com (Carrie Disbrow) Date: Fri, 29 Mar 2024 15:36:40 +0000 Subject: [Histonet] Plastic embedding and cutting training (Charles Riley) In-Reply-To: References: Message-ID: Hi Charles, I found this. There may be more on Google Scholar using a more specific search. Plastic Embedding and Sectioning of Xenopus laevis Embryos YouTube * Educational courses 14 minutes, 40 seconds Apr 18, 2022 ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, March 28, 2024 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 244, Issue 15 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Plastic embedding and cutting training (Charles Riley) 2. certified/uncertified (Nancy Schmitt) 3. Re: certified/uncertified (Whitaker, Bonnie) 4. Reagent alcohols and tissue processors (Paula Sicurello) 5. Re: [EXT] Reagent alcohols and tissue processors (Kara, Phillip) 6. Re: [EXT] Reagent alcohols and tissue processors (Paula Sicurello) 7. ThrowBack Thursday - Throwback to the last time you updated your resume! Day 4 of RELIA Resume Tune-UP Week! (relia1 at earthlink.net) 8. Re: [EXT] Reagent alcohols and tissue processors (Colleen Forster) ---------------------------------------------------------------------- Message: 1 Date: Wed, 27 Mar 2024 14:35:06 -0400 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Plastic embedding and cutting training Message-ID: Content-Type: text/plain; charset="UTF-8" Hello all, I am a research histologist at the University of Delaware. My core is looking to bring on plastic embedding and microtomy within the next year. I am trying to find an online training course to learn the process and or if anyone along the East Coast near Delaware would be willing to let me come tour your facility and shadow you sometime that would be great. So any assistance or suggestions would be greatly appreciated. ------------------------------ Message: 2 Date: Wed, 27 Mar 2024 19:57:42 +0000 From: Nancy Schmitt To: Histonet Subject: [Histonet] certified/uncertified Message-ID: Content-Type: text/plain; charset="us-ascii" Hello- Looking for current practices with hiring: if you were to hire a person with BS that has already been trained in histology but is not certified - would they be hired at same rate as certified tech? would they be expected to get certified? It's a slippery slope....... Thanks Nancy Schmitt HT, MLT(ASCP) Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ------------------------------ Message: 3 Date: Wed, 27 Mar 2024 20:09:33 +0000 From: "Whitaker, Bonnie" To: Nancy Schmitt , Histonet Subject: Re: [Histonet] certified/uncertified Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Nancy, If someone is certification eligible, we hire them into the same position (for us it's a C2 level Histotechnologist) and they have one year to get certified. The pay would be the bottom off the range, with a raise when they obtain their certification. If they don't have the requisite experience, we hire them in as a C1. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Nancy Schmitt via Histonet Sent: Wednesday, March 27, 2024 3:58 PM To: Histonet Subject: [Histonet] certified/uncertified Hello- Looking for current practices with hiring: if you were to hire a person with BS that has already been trained in histology but is not certified - would they be hired at same rate as certified tech? would they be expected to get certified? It's a slippery slope....... Thanks Nancy Schmitt HT, MLT(ASCP) Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!Dzt8CrcZjYAK_3cE_nkD-2iArUt8vOjDA9R_FhIhNCRDNQ2IS-H3HbYFOgVH4Fe4LZKh1SRYDgqvKhKlkbcTq4fUqMxHRKx-OHIySw$ ------------------------------ Message: 4 Date: Thu, 28 Mar 2024 13:04:33 +0000 (UTC) From: Paula Sicurello To: HistoNet Subject: [Histonet] Reagent alcohols and tissue processors Message-ID: <472887967.2172123.1711631073040 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer ------------------------------ Message: 5 Date: Thu, 28 Mar 2024 13:14:13 +0000 From: "Kara, Phillip" To: HistoNet , Paula Sicurello Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors Message-ID: Content-Type: text/plain; charset="us-ascii" So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs. Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times. Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 p: 918-281-9060 w: www.unthsc.edu/corelabs> ________________________________ From: Paula Sicurello via Histonet Sent: Thursday, March 28, 2024 8:04 AM To: HistoNet Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70% but I favor a lower %. One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 ------------------------------ Message: 6 Date: Thu, 28 Mar 2024 14:14:50 +0000 (UTC) From: Paula Sicurello To: Phillip.Kara at unthsc.edu, HistoNet Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors Message-ID: <544715091.2194403.1711635290080 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Hi Kara, I do like the VIPs.? ?Besides getting rid of the NBF, the alcohols are getting rid of the water as well. I learned EM first, so my processing thoughts are probably biased by that. Paula? Yahoo Mail: Search, Organize, Conquer On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs.Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never had any issues with the tissues starting at 70. I get the idea of starting lower but you are also then going to need to increase your processing time which can delay turnaround times.Plus correct me if I am wrong but isn't the whole point of the alcohols after NBF to get the NBF out of the tissue and ready for xylene and wax? Phillip Kara, HTL | Senior Research Associate University of North Texas Health Science Center Division of Research and Innovation? a:?3500 Camp Bowie Blvd, Fort Worth, TX 76107 p:?918-281-9060 w:www.unthsc.edu/corelabs From: Paula Sicurello via Histonet Sent: Thursday, March 28, 2024 8:04 AM To: HistoNet Subject: [EXT] [Histonet] Reagent alcohols and tissue processors?Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%? but I favor a lower %.? One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for your insight, Paula Yahoo Mail: Search, Organize, Conquer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 ------------------------------ Message: 7 Date: Thu, 28 Mar 2024 12:18:36 -0400 From: To: Subject: [Histonet] ThrowBack Thursday - Throwback to the last time you updated your resume! Day 4 of RELIA Resume Tune-UP Week! Message-ID: <01ee01da812b$974f1490$c5ed3db0$@earthlink.net> Content-Type: text/plain; charset="us-ascii" Hi Histopeeps! Happy Thursday! Let's Throwback Thursday to the last time you updated your resume! Yep It's Day 4 of RELIA Resume Tune-up WEEK! I have already tuned up some resumes for some amazing histopeeps and am ready for more! REMEMBER! There are lots of reasons to have an updated resume! **Prepping for a performance review? **Preparing for a presentation? **Transitioning between travel and perm work? **Going for a promotion? **Going Back to School? **Going to a Networking Event? **Going to give a Workshop? **Going to Run for Office? **Going to Volunteer for a Cause? Your Updated RESUME is an Investment in YOUR Personal Brand! It's Free and It's simple. All you have to do is send me an email to relia1 at earthlink.net with the subject line: "Im in" ! Histopeeps, Let me help you get your resume tuned up!! It's Free of charge to my histopeeps! #ThrowbackThursday #Thursday #ThursdayThoughts #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ------------------------------ Message: 8 Date: Thu, 28 Mar 2024 11:55:04 -0500 From: Colleen Forster To: Paula Sicurello Cc: Phillip.Kara at unthsc.edu, HistoNet Subject: Re: [Histonet] [EXT] Reagent alcohols and tissue processors Message-ID: Content-Type: text/plain; charset="UTF-8" Paula, I start my processor at 80%. I am in research and often the samples have been fixed and are put into 70-80% ethanol for holding or transport. Starting with 80% steps right in line with the samples. I rarely see a schedule that starts at 50%. However, I do have a couple labs that ask me for that. I am fortunate to have a second processor I can use for special programs and not hold up my main runs. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory On Thu, Mar 28, 2024 at 9:15?AM Paula Sicurello via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Kara, > I do like the VIPs. Besides getting rid of the NBF, the alcohols are > getting rid of the water as well. > I learned EM first, so my processing thoughts are probably biased by that. > Paula > > Yahoo Mail: Search, Organize, Conquer > > On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip > wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to > use, easy maintenance, and very customizable for programed runs.Every lab I > have been in has always started at 70. It saves the techs extra time mixing > up dilutions because you can always find RTU 70%. It also helps I have > never had any issues with the tissues starting at 70. I get the idea of > starting lower but you are also then going to need to increase your > processing time which can delay turnaround times.Plus correct me if I am > wrong but isn't the whole point of the alcohols after NBF to get the NBF > out of the tissue and ready for xylene and wax? > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w:www.unthsc.edu/corelabs > > From: Paula Sicurello via Histonet > Sent: Thursday, March 28, 2024 8:04 AM > To: HistoNet > Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good > Morning, > My question two part: > Best tissue processor ever and why? > What is percentage is your first alcohol step? > I see that lots of places start at 70% but I favor a lower %. One > because it's gentler on the tissue, and two because 10% NBF precipitates > out if you start at 70%. > Thanks for your insight, > Paula > > Yahoo Mail: Search, Organize, Conquer > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cphillip.kara%40unthsc.edu%7Cbb0d8a2788464841512508dc4f27b344%7C70de199207c6480fa318a1afcba03983%7C0%7C0%7C638472279141493958%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=BaFYUI%2FlU5jHIS8xsMkXDj9At%2FONw0U27DhTT6WbYBw%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 244, Issue 15 ***************************************** From relia1 at actsend.com Fri Mar 29 11:16:44 2024 From: relia1 at actsend.com (Pam Barker) Date: Fri, 29 Mar 2024 16:16:44 +0000 Subject: [Histonet] Have A Wonderful Holiday Weekend and ICYMI Here's the info on State Meetings!! Message-ID: <90.5B.42321.C69E6066@kf.mta1vrest.cc.prd.sparkpost> Hi Histopeeps ! It?s Finally Friday!? Good Friday for some! I wish you well!??We can all agree?? It?s a great time to have a Wonderful Weekend! I hope you do! ICYMI! Interested in attending a state histology society meeting? Check your state's website or social media page! Check in our groups on Facebook and LinkedIn! All of the info is there! There are some AMAZING meetings being held this Spring! It's a fantastic way to connect to your community. Learn and earn CEUs! And It's FUN!!!! If you need info for your local meeting contact me ASAP! I will get it to you! There are meetings being held in: ? Florida ? Georgia ? Texas ? Massachusetts ? Ohio ? Wisconsin/Illinois (These are the ones that I know of) And the National Meeting is in New Orleans in September! Histopeeps!!!? Check It Out! ? If you are looking for a new opportunity, I can help with that too! ? If you are looking for a fantastic addition to your team, I can help with that too!! ? If you need a resume tune-up, I can help with that too! Email me relia1 at earthlink.net Call/text me at 407-353-5070. Have a Wonderful Weekend! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone:????? (407)657-2027 Cell:?????? (407)353-5070 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology? Click Here to Unsubscribe? 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