From relia1 at actsend.com Wed Jun 5 12:26:23 2024 From: relia1 at actsend.com (Pam Barker) Date: Wed, 05 Jun 2024 17:26:23 +0000 Subject: [Histonet] Happy Hump Daaay Histopeeps! We're Halfway Through The Week and The YEAR!! Message-ID: <88.01.30899.FBF90666@jl.mta1vrest.cc.prd.sparkpost> Happy Hump Daaaay Histopeeps! We Are Halfway To The WEEKEND!!! AND Halfway Through 2024!! My Best Clients Are Engaging Me For Amazing Histology Opportunities? For The Summer And The Rest Of 2024! If You Are Even CONSIDERING A Change Big Or Small In 2024 WE NEED TO TALK! I Might Have A Great Opportunity NOW Or LATER When The Timing Is Better. THINKING About Travel Histology? ?????????? THINK AGAIN!! Rates Are Falling And Good Contracts Are Drying Up! ON THE OTHER HAND, You Might Want To Consider A Permanent Position. >>I have Permanent Positions that Pay More Than Travel! >>Some Clients Are Offering Sign On Bonuses Up To 20k I have Fantastic leadership and technical positions! My clients are located Nationwide including: Virginia Florida Georgia Wisconsin Illinois Indiana California Arizona South Dakota And I Am Getting Shiny Brand New Opportunities Coming In EVERYDAY From EVERYWHERE In The U.S.A!! For More INFO: Email Me: Relia1 at Earthlink.Net Call/Text Me :407-353-5070 Have a Great Day! Thanks-Pam! Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community!? Thank You! ?Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ? Click Here to Unsubscribe? RELIA Solutions? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? histonet at lists.utsouthwestern.edu From kate.bummer at seqmatic.com Fri Jun 7 09:52:20 2024 From: kate.bummer at seqmatic.com (Kate Bummer) Date: Fri, 7 Jun 2024 14:52:20 +0000 Subject: [Histonet] What is the best xylene substitute for histology? Message-ID: Hello Histonetters I'm hoping I can get some recommendations for the best xylene substitute for histology that could be used in the following: * Tissue processor * Deparaffinization * Coverslipper * Mounting media that works with that particular xylene substitute Thank you for your help! Kate SeqMatic From jmacdonald at mtsac.edu Fri Jun 7 13:47:43 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Fri, 7 Jun 2024 18:47:43 +0000 Subject: [Histonet] What is the best xylene substitute for histology? In-Reply-To: References: Message-ID: We have used both XS-3 and Clear-Rite 3 with success in processing and staining. They are not as efficient as xylene, so you will need to extend the times a bit to account for that. We have had the most success with Permount. No fading or incompatibility. Jennifer -----Original Message----- From: Kate Bummer via Histonet Sent: Friday, June 7, 2024 7:52 AM To: histonet at lists.utsouthwestern.edu Cc: Kate Bummer Subject: [Histonet] What is the best xylene substitute for histology? EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hello Histonetters I'm hoping I can get some recommendations for the best xylene substitute for histology that could be used in the following: * Tissue processor * Deparaffinization * Coverslipper * Mounting media that works with that particular xylene substitute Thank you for your help! Kate SeqMatic _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Fri Jun 7 17:05:28 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 7 Jun 2024 22:05:28 +0000 Subject: [Histonet] What is the best xylene substitute for histology? In-Reply-To: References: Message-ID: I liked terpineol (mixed isomers). Smells nice and doesn't harden tissues, but I see that it has now become pretty expensive. Mineral oil USP (also called liquid paraffin and mineral oil, heavy) is OK and cheaper than xylene, but it's not miscible with ethanol. You have to dehydrate specimens in isopropanol and have the oil warmer than 44oC. Tert-butanol is miscible with water, ethanol and paraffin; it's somewhat more expensive than xylene and must be used warm because it freezes at 26oC. It's often used for plant histology. A recently proposed safe and economical alternative clearing agent for paraffin embedding is coconut oil, but it causes much more shrinkage than xylene and also induces ugly artifacts (in rats' prostates) if the clearing time is less than 4 hours. See OA Bright et al. 2024. J. Histochem. Cytochem. 72(4): 233-243. https://www.researchgate.net/profile/Ebenezer-Senu/publication/379429541_Clearing_Properties_Between_Coconut_Oil_and_Xylene_in_Histological_Tissue_Processing/links/661013eb2034097c54f61dbd/Clearing-Properties-Between-Coconut-Oil-and-Xylene-in-Histological-Tissue-Processing.pdf Just a few thoughts! John Kiernan. London, Canada ________________________________ From: Kate Bummer via Histonet Sent: June 7, 2024 10:52 AM To: histonet at lists.utsouthwestern.edu Cc: Kate Bummer Subject: [Histonet] What is the best xylene substitute for histology? Hello Histonetters I'm hoping I can get some recommendations for the best xylene substitute for histology that could be used in the following: * Tissue processor * Deparaffinization * Coverslipper * Mounting media that works with that particular xylene substitute Thank you for your help! Kate SeqMatic _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sat Jun 8 13:01:29 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 8 Jun 2024 18:01:29 +0000 Subject: [Histonet] What is the best xylene substitute for histology? Message-ID: As usual, great insights/advice from JK I always learn from you, Sir You are THE person that I "listen" to ( you may have stood on the shoulders of others?? Do tell) I'm always looking for xylene substitutes.....haven't found any as efficient....sigh I run the gamut with my Safety people regularly but.....they don't understand Then they just "disappear" each year.... Not good, I know.... I run a Leica "dipN dunk" processor ( Leica TP1020) Been using it for 20 yrs without problems...phew! However.....many diagnostic labs use ( far more expensive) substitutes effectively.... Homing in to this thread/topic....avidly Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From idimenstein at hotmail.com Sun Jun 9 18:20:35 2024 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Sun, 9 Jun 2024 23:20:35 +0000 Subject: [Histonet] Histonet Digest, Vol 247, Issue 4 In-Reply-To: References: Message-ID: What about Rene J. Buesa Histology without Xyline. Annals of Diagnostic Pathology, 05 Feb 2009, 13(4):246-256 https://doi.org/10.1016/j.anndiagpath.2008.12.005 PMID: 19608083 Share this article Share with emailShare with twitterShare with linkedinShare with facebook ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Sunday, June 9, 2024 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 247, Issue 4 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7C%7C7b54bcf4f9a7489168b408dc88a59c6a%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C638535492143032968%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=K8o7vgEjK2QEZU2ULRvADXqqFCCD1PzMVVXVD4TdHU4%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From relia1 at earthlink.net Mon Jun 10 11:58:55 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 10 Jun 2024 12:58:55 -0400 Subject: [Histonet] Happy Monday!! RELIA Summer Kick OFF!! It Start's THIS WEEK! Message-ID: <019d01dabb57$7b1ce110$7156a330$@earthlink.net> Happy Monday Histopeeps! Let's make this week AWESOME! Stay tuned because Wednesday is: RELIA's Summer KickOFF!! It's my campaign to help you make this the Best Summer EVER! I will only be running this campaign in MY groups for NOW. So please feel free to invite your friends to join ASAP so they don't MISS OUT! So if you don't want to miss it check out the links for my groups: LinkedIN: RELIA Histology Professionals Career Network: https://www.linkedin.com/groups/1773307/ Facebook: Histology - HT or HTL: www.facebook.com/groups/histotechnologists Histology Leadership Network: https://www.facebook.com/groups/1601326346948216 Histology Professionals Career Network: https://www.facebook.com/groups/reliasolutions Have a Fantastic Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Wed Jun 12 12:17:00 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 12 Jun 2024 13:17:00 -0400 Subject: [Histonet] KickOFF Your Summer with RELIA! Message-ID: <000c01dabcec$57645d70$062d1850$@earthlink.net> Happy Wednesday Histopeeps! Today Is The Day! Relia's Summer Kickoff Starts Today!! Stage One! I Have Set Up A Chat In Our Groups. They Are: * Admin Requests * Ask A Recruiter - Career Advice I Will Be Monitoring The Chats And Am Always Available There In The Chats Or Msg Me Directly On Social Media Email: Relia1 at Earthlink.Net Cell/Text: 407-353-5070 Share The Wealth And Tag Your Friends!! ICYMI These Features Are Only Available In These Facebook Groups! Histology Leadership: Https://Www.Facebook.Com/Groups/1601326346948216 Histology Professionals Career Network Https://Www.Facebook.Com/Groups/Reliasolutions Histology Ht Or HTL ASCP Https://Www.Facebook.Com/Groups/Histotechnologists * I Also Have Amazing Opportunities Both Technical And Leadership! * My Opportunities Are With The Best Employers In Histology! * I Don't Do Big Box Anonymous Labs! You Can Find Those Positions If You Want Them. * Most Of My Positions Are Permanent With Small To Medium Size Labs That Offer Growth And Opportunity. I also Work With The Histology Vendors And Some Research Organizations That's Right You Might Never See The Positions I Am Working On Unless You Connect With Me. Msg Me Directly On Social Media Email: Relia1 at Earthlink.Net Cell/Text: 407-353-5070 Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From redrose297 at gmail.com Wed Jun 12 13:41:20 2024 From: redrose297 at gmail.com (warda hassan) Date: Wed, 12 Jun 2024 22:41:20 +0400 Subject: [Histonet] Special stainer Message-ID: Hello, We are planning to purchase a special stainer for our pathology lab. The staining panel mainly consists of liver, renal, bone marrow, skin, and GI samples. Based on your experience, feedback, and troubleshooting, which one would you recommend among these three: Leica, Roche, and Dako? Thank you. From relia1 at actsend.com Thu Jun 13 10:28:13 2024 From: relia1 at actsend.com (Pam Barker) Date: Thu, 13 Jun 2024 15:28:13 +0000 Subject: [Histonet] RELIA Hot Leadership Alert! Opportunities to lead and grow and more! Message-ID: <5A.15.17822.D001B666@kk.mta1vrest.cc.prd.sparkpost> Happy Thursday?Histopeeps? ! I Hope You Have Been Having A Great Week! I Am So Excited!!! I Have These Amazing Opportunities For You And Your Fellow Histopeeps! I Have Opportunities For Leadership And Growth! My Clients Are Looking For: ? Directors ? Managers ? Supervisors ? Lead Techs And Most Importantly My Clients Are Looking For Histotechs Who Are Ready To ? Learn ? Lead ? Grow ? Prosper I Have Opportunities Nationwide Including In: ???????? Florida ???????? Georgia ???????? Wisconsin ???????? South Dakota ???????? Illinois ???????? Virginia ???????? Indiana ???????? Arizona Histopeeps,??I Also Have Amazing Opportunities Both Technical And Leadership Coming In Daily! ? My Opportunities Are With The Best Employers In Histology! ? I Don't Do Big Box Anonymous Labs!? You Can Find Those Positions If You Want Them. ? Most Of My Positions Are Permanent With Small To Medium Size Labs That Offer Growth And Opportunity.? ? I Also Work With The Histology Vendors And Some Research Organizations ? That's Right You Might Never See These Amazing Opportunities Unless You Read My Emails? Because MOST Of Them are RELIA EXCLUSIVES!! Have A Fantastic Day!!! Thanks-Pam? Right Time, Right Place, Right Move With RELIA! Providing Excellent Service Exclusively To The Histology Community!? Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists In Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, Fl 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 Toll Free: (866)60relia Or (866)607-3542 E-Mail: Relia1 at Earthlink.Net? #Ilovemyhistopeeps #Jobs4myhistopeeps #Histologyiscool #Histologyjobs #Histologycareers #Histology Click Here to Unsubscribe? RELIA Solutions? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? histonet at lists.utsouthwestern.edu From relia1 at earthlink.net Fri Jun 14 11:59:58 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 14 Jun 2024 12:59:58 -0400 Subject: [Histonet] =?utf-8?b?8J+MnyBSRUxJQeKAmVMgSGlzdG9wZWVwIFN1bW1lciBT?= =?utf-8?b?cGxhc2ghISDwn4yf?= Message-ID: <000a01dabe7c$4fa35fa0$eeea1ee0$@earthlink.net> ? RELIA?S Histopeep Summer Splash!! ? ? Calling All Histopeeps! ? Are You Ready To Make A Mark In The World Of Histology This Summer? ? We?re Super Excited To Unveil RELIA?s Histopeep Summer Splash! It?s Not Just A Campaign; It?s A Gateway To Your Future In Histological Excellence. ? Circle Monday on your calendar ? that?s when we?re launching into action, and you?re invited to join the adventure! But wait, there?s more! * Weekly AI Histology Art Giveaway! * New Blog Posts covering Pertinent topics for Job Seekers, Employers AND Histopeeps who are happy where they are * Exciting job opportunities that are exclusive to RELIA! * A Reward Referral Program for referring Job Seekers AND Job Openings! * Resume Tune-Ups! * Interview Coaching - Even if you are not pursuing one of my jobs! * Career Strategy - Happy where you are but want to go further there? I can help with that too! ? Engage with us by sharing your histology dreams and questions using #HistologyJourney. Your story could be featured! ? Connect with peers, learn from the best, and discover career paths that will ignite your passion for histology- Here?s HOW! Subscribe to my Histology Careers Bulletin by sending me an email to relia1 at earthlink.net OR Join One of My Groups On Social Media! Facebook * Histology Leadership: * Https://Www.Facebook.Com/Groups/1601326346948216 * Histology Professionals Career Network * Https://Www.Facebook.Com/Groups/Reliasolutions * Histology Ht Or HTL ASCP * Https://Www.Facebook.Com/Groups/Histotechnologists LinkedIN RELIA Histology Professionals Career Network: https://www.linkedin.com/groups/1773307/ Don?t Just Watch From The Sidelines ? Be Part Of The Movement That?s Redefining Careers IN Histological Research And Practice. #FollowFriday #TGIF #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers #HistologyRecruiting #Summer2024 #ScienceJobs #HistologyJourney Have a Great Weekend! Thanks - Pam From relia1 at earthlink.net Mon Jun 17 11:00:07 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 17 Jun 2024 12:00:07 -0400 Subject: [Histonet] RELIA's Histopeep Summer Splash Starts TODAY! Don't Miss OUT! Message-ID: <004401dac0cf$6d9c6420$48d52c60$@earthlink.net> RELIA's Histopeep Summer Splash Starts TODAY! Happy Monday Histopeeps! ! Let's make this week AWESOME! ICYMI: RELIA's Histopeep Summer Splash Starts TODAY! Back By Popular Demand and MANY Recent Requests! RESUME TUNE-UPS Y'all! That's right! I will tune up your resume for you. Things You Might Need A Resume For in 2024! A Job Search A Promotion An InterCompany Move A Publication A Presentation/Workshop A Great Way To See What You have Accomplished! This as always is Free of charge to my histopeeps. Just shoot me an email to relia1 at earthlink.net with a copy of your resume preferably in Word and I will be happy to take a look at it. WHY? Because I Love My Histopeeps! Join one of my groups for even more FUN! For Example in my groups: A Free Histology Art Giveaway! This will only be available to histopeeps in my groups and on my subscribers list: Subscribe to my Histology Careers Bulletin by sending me an email to relia1 at earthlink.net OR Join One of My Groups On Social Media! Facebook ? Histology Leadership: ? Https://Www.Facebook.Com/Groups/1601326346948216 ? Histology Professionals Career Network ? Https://Www.Facebook.Com/Groups/Reliasolutions ? Histology Ht Or HTL ASCP ? Https://Www.Facebook.Com/Groups/Histotechnologists LinkedIN RELIA Histology Professionals Career Network: https://www.linkedin.com/groups/1773307/ WHY? - Because I can't post images on the histonet y'all! Have a great Monday! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Tue Jun 18 11:52:25 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 18 Jun 2024 12:52:25 -0400 Subject: [Histonet] RELIA's Summer Splash For Histopeeps Starts TODAY! Message-ID: <007501dac19f$ef2e9820$cd8bc860$@earthlink.net> Happy Tuesday Treat Histopeeps! I am so excited because today is the DAY we kick off RELIA?s Histopeep Summer Splash! It's my campaign to help you make this the Best Summer EVER! Here?s What We Are Doing: ? Each Week I will be sharing an AI created piece of Histology Art. I hope you like it, save it and share it. ? Each Week I will be sharing some career advice in the form of a blog post or article And Free Resume Tune-ups! I hope you enjoy my emails and YOUR Summer!! This Week?s AI Histology Art is A Histology Stain Beach Ball! Can?t post pics on the histonet but shoot me an email to relia1 at earthlink.net and I will send it to YOU! OR Join my group on Facebook: www.facebook.com/groups/histotechnologists Feel free to like save and share! I will also be featuring my BEST Histology Opportunities!! Here Are Some of My MOST Exciting Opportunities: ? Most of them are RELIA Exclusives! ? All of Them are Permanent Positions! ? My clients offer very competitive compensation including relocation assistance and sign on bonuses up to 20K ? Travel rates are going DOWN and Perm Pay is Going UP! Leadership: ? Florida ? Georgia ? South Dakota ? New Mexico ? Wisconsin HT/HTL or Elig: ?AZ - Arizona ? Mohs ?FL - Florida ? Cancer Dx ?VA - Virginia ? Education/Clinical ?IL - Illinois - Derm ?TX - Texas ? Derm ?TN ? Tennessee ? Clinical Dx ?IN ? Indiana ? Mohs And I have new opportunities coming in daily so stay tuned! OR Drop ME a line and let me know what to BOLO for!! Check It Out! It Never Hurts To Look! For More Info Contact ME! Email: relia1 at earthlink.net Cell/text: 407-353-5070 Here Is This Week?s Splash: ?Free Resume Tune-Ups ? Just Shoot Me An Email To relia1 at earthlink.net With A Copy Of Your Resume! ?Free Histology Art Giveaway ?Stay Tuned for a fantastic Blog Post This WEEK! Have a Fantastic Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! #RELIASummerSplash2024 #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From cforster at umn.edu Tue Jun 18 17:58:51 2024 From: cforster at umn.edu (Colleen Forster) Date: Tue, 18 Jun 2024 17:58:51 -0500 Subject: [Histonet] PEGsylated cells Message-ID: Hello Histonetters, I have been asked about the use of PEG cells and the idea of conjugating with a nano[-article. In sort the goals: 1-visualize PEG 2 coat in an even layer of PEg (if it is not already) 3 - functionalized PEG - attach nanoparticles to either induce cell infiltration or no clots etc.. Since this is a new concept for me I am reaching out to see if anyone in this group has worked with PEG and can give me references, diorection, protocols... As always, thanks in advance~. Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From greg.dobbin at gmail.com Wed Jun 19 07:53:42 2024 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 19 Jun 2024 09:53:42 -0300 Subject: [Histonet] Causes of false positive Congo Red Message-ID: Hello experts, *Some background:* I know that Congo Red can bind nonspecifically to non-amyloid components such as collagen and elastin under certain conditions (eg Carnoys fixative, insufficient differentiation, insufficient alkalinity, etc). However, everything I have been able to read on the topic suggests that over-staining is "easily" differentiated from true amyloid staining by using polarizing light microscopy. That is, true amyloid produces apple green fluorescence while non-amyloid components produce silver/grey color. *My question:* I want to know if anyone has encountered false positive staining that *is apple green* in color? We had a few bone marrow core biopsies that stained bright green but were later found to be negative when stained at another lab. We subsequently threw out all of our working solutions and made up everything fresh and repeated the previous (false positive) specimens and they were indeed negative in our lab as well. *In order to prevent this from happening again, I need to attempt to understand what may have caused this to happen in the first place. * This is where the vast collective knowledge of this group comes in. :-) Can anyone offer some insight as to possible causes? *Our Congo Red method:* Deparaffinize sections and bring them to water. Stain in Hematoxylin for 1 minute Add 0.5ml of 1% Sodium Hydroxide to 50 ml of stock alkaline salt solution. Wash slides in running water Place in *working* alkaline salt solution from step 2 for 20 minutes Add 0.5 ml of 1% Sodium Hydroxide to stock Congo red solution. Start to filter *working* Congo red solution when 15 mins are left in step 6 Place sections in the *working* Congo red from step #8 for 20 minutes. Dehydrate the slides one at a time in 3 changes of absolute ethanol, 6 dips each. Dip the slide 10 times in a coplin of xylene. Continue dehydrating the other slides. Coverslip the slides. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 From jkiernan at uwo.ca Thu Jun 20 00:49:03 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 20 Jun 2024 05:49:03 +0000 Subject: [Histonet] Causes of false positive Congo Red In-Reply-To: References: Message-ID: Greg, your method is incompletely described in your Histonet post, but it looks quite different from the "traditional" Highman's procedure (Arch. Path. 41:559-562). What method were they using "at another lab" to get correct red amyloid that is green (dichroic, not fluorescent) with crossed polars? John Kiernan https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Greg Dobbin via Histonet Sent: June 19, 2024 8:53 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Causes of false positive Congo Red Hello experts, *Some background:* I know that Congo Red can bind nonspecifically to non-amyloid components such as collagen and elastin under certain conditions (eg Carnoys fixative, insufficient differentiation, insufficient alkalinity, etc). However, everything I have been able to read on the topic suggests that over-staining is "easily" differentiated from true amyloid staining by using polarizing light microscopy. That is, true amyloid produces apple green fluorescence while non-amyloid components produce silver/grey color. *My question:* I want to know if anyone has encountered false positive staining that *is apple green* in color? We had a few bone marrow core biopsies that stained bright green but were later found to be negative when stained at another lab. We subsequently threw out all of our working solutions and made up everything fresh and repeated the previous (false positive) specimens and they were indeed negative in our lab as well. *In order to prevent this from happening again, I need to attempt to understand what may have caused this to happen in the first place. * This is where the vast collective knowledge of this group comes in. :-) Can anyone offer some insight as to possible causes? *Our Congo Red method:* Deparaffinize sections and bring them to water. Stain in Hematoxylin for 1 minute Add 0.5ml of 1% Sodium Hydroxide to 50 ml of stock alkaline salt solution. Wash slides in running water Place in *working* alkaline salt solution from step 2 for 20 minutes Add 0.5 ml of 1% Sodium Hydroxide to stock Congo red solution. Start to filter *working* Congo red solution when 15 mins are left in step 6 Place sections in the *working* Congo red from step #8 for 20 minutes. Dehydrate the slides one at a time in 3 changes of absolute ethanol, 6 dips each. Dip the slide 10 times in a coplin of xylene. Continue dehydrating the other slides. Coverslip the slides. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greg.dobbin at gmail.com Thu Jun 20 07:31:50 2024 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 20 Jun 2024 09:31:50 -0300 Subject: [Histonet] Causes of false positive Congo Red In-Reply-To: References: Message-ID: Good day John, Very nice to hear from you again! I have been consulting your textbook in my investigations! Sorry about the brevity of the description of our method. I felt like my post was already too long and it might scare off some would-be contributors! :-) And yes, I incorrectly referred to the dichroic green as "fluorescent"-thank you. Our method follows the Puchtler method described on pages 132-3 in Frieda Carson's "Self-Instructional" textbook (1990) as does the hospital that repeated our false-positive Congo Reds. Note, once we re-made our reagents, our results returned to accurate staining. Greg On Thu, Jun 20, 2024 at 2:49?AM John Kiernan wrote: > Greg, your method is incompletely described in your Histonet post, but it > looks quite different from the "traditional" Highman's procedure (*Arch. > Path*. *41*:559-562). What method were they using "at another > lab" to get correct red amyloid that is green (dichroic, not fluorescent) > with crossed polars? > *John Kiernan* > > https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html > = = = > ------------------------------ > *From:* Greg Dobbin via Histonet > *Sent:* June 19, 2024 8:53 AM > *To:* histonet at lists.utsouthwestern.edu > > *Subject:* [Histonet] Causes of false positive Congo Red > > Hello experts, > *Some background:* > I know that Congo Red can bind nonspecifically to non-amyloid components > such as collagen and elastin under certain conditions (eg Carnoys fixative, > insufficient differentiation, insufficient alkalinity, etc). However, > everything I have been able to read on the topic suggests that > over-staining is "easily" differentiated from true amyloid staining by > using polarizing light microscopy. That is, true amyloid produces apple > green fluorescence while non-amyloid components produce silver/grey color. > > *My question:* > I want to know if anyone has encountered false positive staining that *is > apple green* in color? We had a few bone marrow core biopsies that stained > bright green but were later found to be negative when stained at another > lab. We subsequently threw out all of our working solutions and made up > everything fresh and repeated the previous (false positive) specimens and > they were indeed negative in our lab as well. > > *In order to prevent this from happening again, I need to attempt to > understand what may have caused this to happen in the first place. * > > This is where the vast collective knowledge of this group comes in. :-) > Can anyone offer some insight as to possible causes? > > *Our Congo Red method:* > > > Deparaffinize sections and bring them to water. > > Stain in Hematoxylin for 1 minute > > Add 0.5ml of 1% Sodium Hydroxide to 50 ml of stock alkaline salt solution. > > Wash slides in running water > > Place in *working* alkaline salt solution from step 2 for 20 minutes > > Add 0.5 ml of 1% Sodium Hydroxide to stock Congo red solution. > > Start to filter *working* Congo red solution when 15 mins are left in step > 6 > > Place sections in the *working* Congo red from step #8 for 20 minutes. > > Dehydrate the slides one at a time in 3 changes of absolute ethanol, 6 dips > each. > > Dip the slide 10 times in a coplin of xylene. > > Continue dehydrating the other slides. > > Coverslip the slides. > > *Greg Dobbin* > 1205 Pleasant Grove Rd > Route 220 > York, PE C0A 1P0 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From greg.dobbin at gmail.com Thu Jun 20 07:44:26 2024 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 20 Jun 2024 09:44:26 -0300 Subject: [Histonet] Causes of false positive Congo Red In-Reply-To: References: Message-ID: Hi John, I must apologize again. We used to use the method from Carson's book. We now make up the reagents as follows: *Stock alkaline salt solution* Sodium chloride............. 2g Distilled Water............... 20mL Stir until the salt is dissolved, then with continuous stirring on a magnetic stirrer add 80mL of 100% denatured ethanol. Some salt may precipitate out after the ethanol is added. *Working alkaline salt solution * Stock alkaline stock solution...50 ml 1% Sodium Hydroxide????0.5ml Filter and use within 15 minutes *Stock Congo red solution* Congo red................................ 0.1g Stock alkaline salt solution........ 50mL Stir well with the magnetic stirrer and *let stand overnight or for a minimum of 3 hours* if the slides need to be ready the same day that the order was placed. *Working Congo red (Congo red)* Stock Congo red...................... 50ml Sodium hydroxide 1%............... 0.5ml Filter and use within 15 minutes. On Thu, Jun 20, 2024 at 9:31?AM Greg Dobbin wrote: > Good day John, > Very nice to hear from you again! I have been consulting your textbook in > my investigations! > Sorry about the brevity of the description of our method. I felt like my > post was already too long > and it might scare off some would-be contributors! :-) And yes, I > incorrectly referred to the dichroic green as "fluorescent"-thank you. > > Our method follows the Puchtler method described on pages 132-3 in Frieda > Carson's "Self-Instructional" textbook (1990) as does > the hospital that repeated our false-positive Congo Reds. Note, once we > re-made our reagents, our results returned to accurate staining. > Greg > > On Thu, Jun 20, 2024 at 2:49?AM John Kiernan wrote: > >> Greg, your method is incompletely described in your Histonet post, but it >> looks quite different from the "traditional" Highman's procedure (*Arch. >> Path*. *41*:559-562). What method were they using "at another >> lab" to get correct red amyloid that is green (dichroic, not fluorescent) >> with crossed polars? >> *John Kiernan* >> >> https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html >> = = = >> ------------------------------ >> *From:* Greg Dobbin via Histonet >> *Sent:* June 19, 2024 8:53 AM >> *To:* histonet at lists.utsouthwestern.edu < >> histonet at lists.utsouthwestern.edu> >> *Subject:* [Histonet] Causes of false positive Congo Red >> >> Hello experts, >> *Some background:* >> I know that Congo Red can bind nonspecifically to non-amyloid components >> such as collagen and elastin under certain conditions (eg Carnoys >> fixative, >> insufficient differentiation, insufficient alkalinity, etc). However, >> everything I have been able to read on the topic suggests that >> over-staining is "easily" differentiated from true amyloid staining by >> using polarizing light microscopy. That is, true amyloid produces apple >> green fluorescence while non-amyloid components produce silver/grey color. >> >> *My question:* >> I want to know if anyone has encountered false positive staining that *is >> apple green* in color? We had a few bone marrow core biopsies that stained >> bright green but were later found to be negative when stained at another >> lab. We subsequently threw out all of our working solutions and made up >> everything fresh and repeated the previous (false positive) specimens and >> they were indeed negative in our lab as well. >> >> *In order to prevent this from happening again, I need to attempt to >> understand what may have caused this to happen in the first place. * >> >> This is where the vast collective knowledge of this group comes in. :-) >> Can anyone offer some insight as to possible causes? >> >> *Our Congo Red method:* >> >> >> Deparaffinize sections and bring them to water. >> >> Stain in Hematoxylin for 1 minute >> >> Add 0.5ml of 1% Sodium Hydroxide to 50 ml of stock alkaline salt solution. >> >> Wash slides in running water >> >> Place in *working* alkaline salt solution from step 2 for 20 minutes >> >> Add 0.5 ml of 1% Sodium Hydroxide to stock Congo red solution. >> >> Start to filter *working* Congo red solution when 15 mins are left in >> step 6 >> >> Place sections in the *working* Congo red from step #8 for 20 minutes. >> >> Dehydrate the slides one at a time in 3 changes of absolute ethanol, 6 >> dips >> each. >> >> Dip the slide 10 times in a coplin of xylene. >> >> Continue dehydrating the other slides. >> >> Coverslip the slides. >> >> *Greg Dobbin* >> 1205 Pleasant Grove Rd >> Route 220 >> York, PE C0A 1P0 >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From bcooper at chla.usc.edu Fri Jun 21 16:10:11 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 21 Jun 2024 21:10:11 +0000 Subject: [Histonet] Microscope slide storage cabinets? Message-ID: Happy Friday Histonet! What is everyone using for long term microscope slide storage these days? Our custom slide drawers in electronic shelving units are almost at capacity, and I need to move a LOT of old slides offsite. All of our REALLY old slides are in "Technicon" style metal drawers. Is there anything better on the market? We won't be making cardboard boxes . . . Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From jkiernan at uwo.ca Sat Jun 22 00:25:35 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 22 Jun 2024 05:25:35 +0000 Subject: [Histonet] Causes of false positive Congo Red In-Reply-To: References: Message-ID: Dear Greg, This is the same as on p.126 in my copy of Carson's 2nd edition (1997) and also in the 11th and last (2008) edn of Churukian's "Manual of the Special Stains Laboratory". The only stock solution that can be expected to change with time is the Stock Congo red solution, because solutions of dyes with large anions are unstable in the presence of inorganic salts. Churukian (p.195) said it could be kept for 2 months. The correct staining you got with newly made solutions suggests that your earlier stock Congo red stock solution was too old. Evidently you solved the problem yourself! In their "Troubleshooting Histology Stains" book, Horobin & Bancroft (1998, p.45-47) stressed the need for a freshly made dye solution. They also suggested ignoring "pink background" and checking that it's not dichroic. They also listed various Congo-positive and dichroic materials that aren't amyloid. An unidentified yellow compound is often present in Congo red and it may sometimes cause generalized yellow background staining. John Kiernan https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Greg Dobbin Sent: June 20, 2024 8:44 AM To: John Kiernan ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Causes of false positive Congo Red You don't often get email from greg.dobbin at gmail.com. Learn why this is important Hi John, I must apologize again. We used to use the method from Carson's book. We now make up the reagents as follows: Stock alkaline salt solution Sodium chloride............. 2g Distilled Water............... 20mL Stir until the salt is dissolved, then with continuous stirring on a magnetic stirrer add 80mL of 100% denatured ethanol. Some salt may precipitate out after the ethanol is added. Working alkaline salt solution Stock alkaline stock solution...50 ml 1% Sodium Hydroxide????0.5ml Filter and use within 15 minutes Stock Congo red solution Congo red................................ 0.1g Stock alkaline salt solution........ 50mL Stir well with the magnetic stirrer and let stand overnight or for a minimum of 3 hours if the slides need to be ready the same day that the order was placed. Working Congo red (Congo red) Stock Congo red...................... 50ml Sodium hydroxide 1%............... 0.5ml Filter and use within 15 minutes. On Thu, Jun 20, 2024 at 9:31?AM Greg Dobbin > wrote: Good day John, Very nice to hear from you again! I have been consulting your textbook in my investigations! Sorry about the brevity of the description of our method. I felt like my post was already too long and it might scare off some would-be contributors! :-) And yes, I incorrectly referred to the dichroic green as "fluorescent"-thank you. Our method follows the Puchtler method described on pages 132-3 in Frieda Carson's "Self-Instructional" textbook (1990) as does the hospital that repeated our false-positive Congo Reds. Note, once we re-made our reagents, our results returned to accurate staining. Greg On Thu, Jun 20, 2024 at 2:49?AM John Kiernan > wrote: Greg, your method is incompletely described in your Histonet post, but it looks quite different from the "traditional" Highman's procedure (Arch. Path. 41:559-562). What method were they using "at another lab" to get correct red amyloid that is green (dichroic, not fluorescent) with crossed polars? John Kiernan https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Greg Dobbin via Histonet > Sent: June 19, 2024 8:53 AM To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Causes of false positive Congo Red Hello experts, *Some background:* I know that Congo Red can bind nonspecifically to non-amyloid components such as collagen and elastin under certain conditions (eg Carnoys fixative, insufficient differentiation, insufficient alkalinity, etc). However, everything I have been able to read on the topic suggests that over-staining is "easily" differentiated from true amyloid staining by using polarizing light microscopy. That is, true amyloid produces apple green fluorescence while non-amyloid components produce silver/grey color. *My question:* I want to know if anyone has encountered false positive staining that *is apple green* in color? We had a few bone marrow core biopsies that stained bright green but were later found to be negative when stained at another lab. We subsequently threw out all of our working solutions and made up everything fresh and repeated the previous (false positive) specimens and they were indeed negative in our lab as well. *In order to prevent this from happening again, I need to attempt to understand what may have caused this to happen in the first place. * This is where the vast collective knowledge of this group comes in. :-) Can anyone offer some insight as to possible causes? *Our Congo Red method:* Deparaffinize sections and bring them to water. Stain in Hematoxylin for 1 minute Add 0.5ml of 1% Sodium Hydroxide to 50 ml of stock alkaline salt solution. Wash slides in running water Place in *working* alkaline salt solution from step 2 for 20 minutes Add 0.5 ml of 1% Sodium Hydroxide to stock Congo red solution. Start to filter *working* Congo red solution when 15 mins are left in step 6 Place sections in the *working* Congo red from step #8 for 20 minutes. Dehydrate the slides one at a time in 3 changes of absolute ethanol, 6 dips each. Dip the slide 10 times in a coplin of xylene. Continue dehydrating the other slides. Coverslip the slides. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Jun 24 11:13:06 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 24 Jun 2024 12:13:06 -0400 Subject: [Histonet] Discover ASCP certified Histotechs For Your Team! Message-ID: <002401dac651$66db4e30$3491ea90$@earthlink.net> ??Histopeeps, Listen Up!?? Are you on the hunt for skilled and experienced histology professionals? Look no further?I?ve got you covered! ? ? 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Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions From plucas at biopath.org Tue Jun 25 13:22:48 2024 From: plucas at biopath.org (Paula) Date: Tue, 25 Jun 2024 11:22:48 -0700 Subject: [Histonet] LDT's Regulatiions by the FDA Message-ID: <007901dac72c$b0247a40$106d6ec0$@biopath.org> Hello, Has anyone read about the new regulations for the LDTs (Laboratory Developed Tests) that were passed by the FDA? Does anyone have any comments about it? If the new LDT requirements by the FDA don't get overturned, we are looking at a lot of paperwork, labor, and consulting/attorney expenses for running IHC's in 2025 and forward. I've attached an article in the June issue of Laboratory Economics for your reference. The subject starts on page 1, then continues to pages 3 and 4. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group From kdean70 at hotmail.com Tue Jun 25 20:46:12 2024 From: kdean70 at hotmail.com (Ken M) Date: Wed, 26 Jun 2024 01:46:12 +0000 Subject: [Histonet] HSV1/2, CMV, Adenovirus Message-ID: Hi: Anyone who may have HSV1/2, CMV, Treponoma, Adenovirus tissue blocks we would be happy to hear from you. We can trade almost any other rare block you can think of or even buy them if your lab allows this policy. Our research partners need them. Kevin Kdean70 at hotmail.com From histo at pathlab.us Wed Jun 26 10:12:36 2024 From: histo at pathlab.us (Histology) Date: Wed, 26 Jun 2024 15:12:36 +0000 Subject: [Histonet] LDT's Regulatiions by the FDA In-Reply-To: <007901dac72c$b0247a40$106d6ec0$@biopath.org> References: <007901dac72c$b0247a40$106d6ec0$@biopath.org> Message-ID: <3284931e51484f81a4faa4f3ca194da5@pathlab.us> I don't see the attachment. Can you try again? Mehndi Helgren Lab Manager 757-664-7901 Dominion Pathology Labs. 733 Boush St. Suite 200 Norfolk, VA 23510 -----Original Message----- From: Paula via Histonet Sent: Tuesday, June 25, 2024 2:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] LDT's Regulatiions by the FDA Hello, Has anyone read about the new regulations for the LDTs (Laboratory Developed Tests) that were passed by the FDA? Does anyone have any comments about it? If the new LDT requirements by the FDA don't get overturned, we are looking at a lot of paperwork, labor, and consulting/attorney expenses for running IHC's in 2025 and forward. I've attached an article in the June issue of Laboratory Economics for your reference. The subject starts on page 1, then continues to pages 3 and 4. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Wed Jun 26 10:45:25 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 26 Jun 2024 10:45:25 -0500 Subject: [Histonet] LDT's Regulatiions by the FDA In-Reply-To: <3284931e51484f81a4faa4f3ca194da5@pathlab.us> References: <007901dac72c$b0247a40$106d6ec0$@biopath.org> <3284931e51484f81a4faa4f3ca194da5@pathlab.us> Message-ID: Can I ask you to provide an example of a laboratory developed test? So we're all on the same page. A test that you are not buying from a vendor, but just a totally in house protocol, reagents, etc? Using a vendor's test kit off label? Would a simple special stain like a PAS qualify as a LDT if you are making your own reagents and using the AFIP manual for a protocol? If you, for instance, tweak your PAMS impregnation time because one pathologist likes his a little darker, is that an LDT? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Jun 26, 2024 at 10:22?AM Histology via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I don't see the attachment. Can you try again? > > > Mehndi Helgren > Lab Manager > 757-664-7901 > Dominion Pathology Labs. > 733 Boush St. Suite 200 > Norfolk, VA 23510 > > > > -----Original Message----- > From: Paula via Histonet > Sent: Tuesday, June 25, 2024 2:23 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] LDT's Regulatiions by the FDA > > Hello, > > > > Has anyone read about the new regulations for the LDTs (Laboratory > Developed > Tests) that were passed by the FDA? > > Does anyone have any comments about it? > > If the new LDT requirements by the FDA don't get overturned, we are > looking at a lot of paperwork, labor, and consulting/attorney expenses for > running IHC's in 2025 and forward. > > > > I've attached an article in the June issue of Laboratory Economics for > your reference. The subject starts on page 1, then continues to pages 3 and > 4. > > > > Thank you, > > Paula Lucas > > Lab Manager > > Bio-Path Medical Group > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Wed Jun 26 11:11:02 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 26 Jun 2024 12:11:02 -0400 Subject: [Histonet] A Pre-4th of July Message from Pam Barker at RELIA Solutions - Free Resume Tune-UP! Message-ID: <011b01dac7e3$7129a830$537cf890$@earthlink.net> Happy Humpday Histopeeps! Happy Pre- 4th of July The next BIG Thing IS 4th of July Weekend and it's just a week away! I hope you have an amazing celebration!! **Celebrate Your Career Independence with a FREE Resume Tune-Up!** This 4th of July, declare independence from outdated resumes! While the skies light up with fireworks, let's ignite new possibilities in your career with a **complimentary resume makeover**. Resumes are more than just job-seeking tools; they're your ticket to promotions, impactful presentations, and professional milestones. **4th of July Special - Absolutely FREE!** Let Me Help polish your resume and help you stand out in any professional arena. Seize this opportunity to enhance your career narrative without spending a dime. Contact us now and let's make your resume as dazzling as the fireworks! Email me at mailto:relia1 at earthlink.net with the subject line: I'M IN! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? From jmacdonald at mtsac.edu Wed Jun 26 15:28:47 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 26 Jun 2024 20:28:47 +0000 Subject: [Histonet] LDT's Regulatiions by the FDA In-Reply-To: <3284931e51484f81a4faa4f3ca194da5@pathlab.us> References: <007901dac72c$b0247a40$106d6ec0$@biopath.org> <3284931e51484f81a4faa4f3ca194da5@pathlab.us> Message-ID: Histonet does not allow attachments. -----Original Message----- From: Histology via Histonet Sent: Wednesday, June 26, 2024 8:13 AM To: Paula ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] LDT's Regulatiions by the FDA EXTERNAL SENDER - Exercise caution with requests, links, and attachments. I don't see the attachment. Can you try again? Mehndi Helgren Lab Manager 757-664-7901 Dominion Pathology Labs. 733 Boush St. Suite 200 Norfolk, VA 23510 -----Original Message----- From: Paula via Histonet Sent: Tuesday, June 25, 2024 2:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] LDT's Regulatiions by the FDA Hello, Has anyone read about the new regulations for the LDTs (Laboratory Developed Tests) that were passed by the FDA? Does anyone have any comments about it? If the new LDT requirements by the FDA don't get overturned, we are looking at a lot of paperwork, labor, and consulting/attorney expenses for running IHC's in 2025 and forward. I've attached an article in the June issue of Laboratory Economics for your reference. The subject starts on page 1, then continues to pages 3 and 4. Thank you, Paula Lucas Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Wed Jun 26 18:06:07 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 26 Jun 2024 18:06:07 -0500 Subject: [Histonet] LDT's Regulatiions by the FDA In-Reply-To: References: <007901dac72c$b0247a40$106d6ec0$@biopath.org> <3284931e51484f81a4faa4f3ca194da5@pathlab.us> Message-ID: Seems to me they're going after immunos. To my recollection, there are only a very few kits that have FDA approval. I would think it's going to be more a manufacturer problem than a lab problem. I doubt they would go after the labs when they can go after Roche and Agilent (Ventana and Dako). But I could be wrong. On Wed, Jun 26, 2024 at 3:41?PM Mac Donald, Jennifer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Histonet does not allow attachments. > > -----Original Message----- > From: Histology via Histonet > Sent: Wednesday, June 26, 2024 8:13 AM > To: Paula ; histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] LDT's Regulatiions by the FDA > > EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > I don't see the attachment. Can you try again? > > > Mehndi Helgren > Lab Manager > 757-664-7901 > Dominion Pathology Labs. > 733 Boush St. Suite 200 > Norfolk, VA 23510 > > > > -----Original Message----- > From: Paula via Histonet > Sent: Tuesday, June 25, 2024 2:23 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] LDT's Regulatiions by the FDA > > Hello, > > > > Has anyone read about the new regulations for the LDTs (Laboratory > Developed > Tests) that were passed by the FDA? > > Does anyone have any comments about it? > > If the new LDT requirements by the FDA don't get overturned, we are > looking at a lot of paperwork, labor, and consulting/attorney expenses for > running IHC's in 2025 and forward. > > > > I've attached an article in the June issue of Laboratory Economics for > your reference. The subject starts on page 1, then continues to pages 3 and > 4. > > > > Thank you, > > Paula Lucas > > Lab Manager > > Bio-Path Medical Group > > > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang at gmx.at Thu Jun 27 08:35:57 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 27 Jun 2024 15:35:57 +0200 Subject: [Histonet] restaining of IHC-slides Message-ID: <000001dac896$f360d4f0$da227ed0$@gmx.at> Hi! An already stained IHC-slide for a special antibody with HIER as pretreatment (eg 48 min) should be restained with another antibody. For doublestaining one has to do a second short HIER (eg 8 min) to denaturate the detection-antibodies before the second primary antibody. So a certain amount of HIER should be necessary before restaining. But I am an uncertain about the intensitiy of HIER. Is the HIER of the first staining still "active" and we need just a short time? Or should we perform the whole protocol for the restaining with the danger of tissue-damage? How do you handle this problem? Thanks in advance Gudrun Lang Linz, Austria From Diana.Martinez-Longoria at ecrmc.org Thu Jun 27 11:48:03 2024 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Thu, 27 Jun 2024 16:48:03 +0000 Subject: [Histonet] HTL Certification Message-ID: Good day all, I have a question that hopefully I get some guidance on. I have an HT certification, but I wanted to try to study for the HTL certification, but it has been a very long time since I had to study for the boards therefore, I was wondering if you guys that are so knowledgeable can give me guidance on how to pursue my endeavor. I am a type of person that needs structure and an outline on how to study, so preferably I would need like an online school that can help me for the HTL. Any recommendations? Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm, B.S, A.S Laboratory ? Pathology Department 1415 Ross Ave | El Centro, CA 92243 (: 760.339.7267 | *: Diana.Martinez-Longoria at ecrmc.org P Please consider the environment before printing this e-mail Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. From Toni.Rathborne at RWJBH.org Thu Jun 27 12:08:15 2024 From: Toni.Rathborne at RWJBH.org (Rathborne, Toni) Date: Thu, 27 Jun 2024 17:08:15 +0000 Subject: [Histonet] HTL Certification In-Reply-To: References: Message-ID: <6ee7bacd0fdd4807b358795cb2169145@RWJBH.org> Hi Diana, We have had two students complete the program at University of North Dakota. Both passed their certification exam the first time. You can get more information from this link. https://med.und.edu/histotechnology/admission-requirements.html Best of luck finding a program that works for you! Toni ------------------------------------------------------------------------------------ NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information protected by law. It is intended only for use by the named addressee(s). If you are not the intended recipient of this e-mail, any dissemination, distribution or copying of this e-mail and its attachments, if any, is strictly prohibited. If you have received this transmission in error, please immediately notify the sender by telephone or by reply e-mail, and permanently delete this e-mail and the attachments, if any, and destroy any printouts. -----Original Message----- From: Diana Martinez-Longoria via Histonet Sent: Thursday, June 27, 2024 12:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] HTL Certification *** This is an External Email *** Good day all, I have a question that hopefully I get some guidance on. I have an HT certification, but I wanted to try to study for the HTL certification, but it has been a very long time since I had to study for the boards therefore, I was wondering if you guys that are so knowledgeable can give me guidance on how to pursue my endeavor. I am a type of person that needs structure and an outline on how to study, so preferably I would need like an online school that can help me for the HTL. Any recommendations? Thank you, Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm, B.S, A.S Laboratory ? Pathology Department 1415 Ross Ave | El Centro, CA 92243 (: 760.339.7267 | *: Diana.Martinez-Longoria at ecrmc.org P Please consider the environment before printing this e-mail Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender at the phone number above and promptly destroy this e-mail and its attachments. ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!7ELXDq9wPoZgNkgTBbft5wtckTXNFpD9OGZatKuYtm-cDx9EJTfakY7LkIcV__a7Snf2X2yxhbT-WBzJgg5cdgWhCiJAJ0iwGb0$ From katherine.davoli at pitt.edu Thu Jun 27 13:12:56 2024 From: katherine.davoli at pitt.edu (Davoli, Katherine A) Date: Thu, 27 Jun 2024 18:12:56 +0000 Subject: [Histonet] cloudy cornea after Hartman's fix Message-ID: Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative? I'm used to working with mice where, if this happens I didn't notice. Katherine Davoli, MDiv, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts From jaylundgren at gmail.com Thu Jun 27 15:48:53 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 27 Jun 2024 15:48:53 -0500 Subject: [Histonet] HTL Certification In-Reply-To: <6ee7bacd0fdd4807b358795cb2169145@RWJBH.org> References: <6ee7bacd0fdd4807b358795cb2169145@RWJBH.org> Message-ID: Make flashcards, all your esoteric fixatives with recipes, and all your special stains with reagents and what they stain for. Work them both sides till you can get 100% consistently. For example, so you can answer the questions "What is the oxidation step in a GMS?" as well as "Which of these stains uses silver nitrate?" There WILL be a question about which fixative to use or not use with uric acid/gout. There WILL be a question about fire extinguisher types. There you go, I just gave you two free ones. I wouldn't even worry about the photomicrographs. They're so lousy you end up just guessing anyway. You can miss all the photo questions and still pass. By the way, the convention when listing your credentials is to only list your terminal degree, unless you want to point out some certification in another field, like M.D., MBA, or MSN., J.D., or BSN, MPH. Yours would be B.S., HTL, (ASCP) CM. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Jun 27, 2024 at 12:08?PM Rathborne, Toni via Histonet < histonet at lists.utsouthwestern.edu> wrote: > > Hi Diana, > > We have had two students complete the program at University of North > Dakota. Both passed their certification exam the first time. You can get > more information from this link. > > https://med.und.edu/histotechnology/admission-requirements.html > > Best of luck finding a program that works for you! > > Toni > > > > > ------------------------------------------------------------------------------------ > > NOTICE: This e-mail and its attachments, if any, may contain legally > privileged and/or confidential information protected by law. It is intended > only for use by the named addressee(s). If you are not the intended > recipient of this e-mail, any dissemination, distribution or copying of > this e-mail and its attachments, if any, is strictly prohibited. If you > have received this transmission in error, please immediately notify the > sender by telephone or by reply e-mail, and permanently delete this e-mail > and the attachments, if any, and destroy any printouts. > > -----Original Message----- > From: Diana Martinez-Longoria via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Thursday, June 27, 2024 12:48 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] HTL Certification > > > *** This is an External Email *** > > Good day all, > > > I have a question that hopefully I get some guidance on. I have an HT > certification, but I wanted to try to study for the HTL certification, but > it has been a very long time since I had to study for the boards therefore, > I was wondering if you guys that are so knowledgeable can give me guidance > on how to pursue my endeavor. I am a type of person that needs structure > and an outline on how to study, so preferably I would need like an online > school that can help me for the HTL. Any recommendations? > > > Thank you, > > Diana Martinez-Longoria > > El Centro Regional Medical Center > > Lead Histotechnician (ASCP)cm, B.S, A.S > > Laboratory ? Pathology Department > 1415 Ross Ave | El Centro, CA 92243 > > (: 760.339.7267 | *: Diana.Martinez-Longoria at ecrmc.org > > > > P Please consider the environment before printing this e-mail > > > > Confidentiality Notice: This e-mail is for the sole use of the intended > recipient(s) and may contain confidential and privileged information. Any > unauthorized review, use, disclosure, or distribution is prohibited. If you > are not the intended recipient, please contact the sender at the phone > number above and promptly destroy this e-mail and its attachments. > > > > > ECRMC Confidentiality Notice: This e-mail is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, PLEASE contact the > sender and promptly destroy this e-mail and its attachments. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KU82p_qNqnQ!7ELXDq9wPoZgNkgTBbft5wtckTXNFpD9OGZatKuYtm-cDx9EJTfakY7LkIcV__a7Snf2X2yxhbT-WBzJgg5cdgWhCiJAJ0iwGb0$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From afhenwood at outlook.com Thu Jun 27 18:17:26 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Thu, 27 Jun 2024 23:17:26 +0000 Subject: [Histonet] cloudy cornea after Hartman's fix In-Reply-To: References: Message-ID: Hartman's (also known as Davidson's) fixative is sometimes used to reveal lymph nodes in resections where they appear opaque - white. It is not surprising that the tissues of the eye would react the same. I assume that the alcohol causes the bleaching of the tissue (or is it the acetic acid?) - more research needed. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Davoli, Katherine A via Histonet Sent: 28 June 2024 04:12 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cloudy cornea after Hartman's fix Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative? I'm used to working with mice where, if this happens I didn't notice. Katherine Davoli, MDiv, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Thu Jun 27 18:59:35 2024 From: cforster at umn.edu (Colleen Forster) Date: Thu, 27 Jun 2024 18:59:35 -0500 Subject: [Histonet] restaining of IHC-slides In-Reply-To: <000001dac896$f360d4f0$da227ed0$@gmx.at> References: <000001dac896$f360d4f0$da227ed0$@gmx.at> Message-ID: Gudrun, One you have done the HIER it is done. No need top repeat. The one thing is that both antibodies need to have the same buffer. Fro example both citrate or both EDTA. Colleen Forster HT(ASCP)QIHC On Thu, Jun 27, 2024 at 8:36?AM Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi! > > An already stained IHC-slide for a special antibody with HIER as > pretreatment (eg 48 min) should be restained with another antibody. > > For doublestaining one has to do a second short HIER (eg 8 min) to > denaturate the detection-antibodies before the second primary antibody. > > So a certain amount of HIER should be necessary before restaining. But I am > an uncertain about the intensitiy of HIER. > > Is the HIER of the first staining still "active" and we need just a short > time? > > Or should we perform the whole protocol for the restaining with the danger > of tissue-damage? > > > > How do you handle this problem? > > > > Thanks in advance > > Gudrun Lang > > > > Linz, Austria > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From jkiernan at uwo.ca Fri Jun 28 00:14:28 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 28 Jun 2024 05:14:28 +0000 Subject: [Histonet] cloudy cornea after Hartman's fix In-Reply-To: References: Message-ID: The cornea consists of cells (nuclei with plenty of cytoplasmic protein) and parallel bundles (lamellae) of collagen fibres, also protein. It is wonderful that the normal cornea is transparent. Doesn't any fixative make the cornea opaque? Davidson's (=Hartmann's) fixative contains plenty of formaldehyde (to add to and slowly crosslink all protein molecules, including collagen fibrils) but perhaps not quite enough ethanol to rapidly coagulate soluble proteins in cytoplasm. (Davidson's 22.2% v/v EtOH is slightly more than in sherry, madeira or vermouth; about half of that in whisky, gin etc.) The acetic acid (about 10%) can be expected to make nuclear DNA molecules and their associated proteins shrink into the patterns we see as typical of different cell-types. Most of the many formalin-acetic-alcohol fixatives contain enough ethanol to bring about protein coagulation before the more slowly reacting formaldehyde does its stuff. As Tony points out, more research is needed. John Kiernan https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Tony Henwood via Histonet Sent: June 27, 2024 7:17 PM To: histonet at lists.utsouthwestern.edu ; Davoli, Katherine A Subject: Re: [Histonet] cloudy cornea after Hartman's fix Hartman's (also known as Davidson's) fixative is sometimes used to reveal lymph nodes in resections where they appear opaque - white. It is not surprising that the tissues of the eye would react the same. I assume that the alcohol causes the bleaching of the tissue (or is it the acetic acid?) - more research needed. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Davoli, Katherine A via Histonet Sent: 28 June 2024 04:12 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] cloudy cornea after Hartman's fix Anyone know why the cornea of my pig eye got white/cloudy on dropping it in to Hartman's fixative? I'm used to working with mice where, if this happens I didn't notice. Katherine Davoli, MDiv, HTL(ASCP)cm (they/them/theirs) Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of Ophthalmology 7.373 UPMC Mercy Pavilion 1622 Locust St., Pittsburgh PA 15219 (412) 624-8508 this number cannot receive texts _______________________________________________ From rsrichmond at gmail.com Fri Jun 28 13:26:16 2024 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 28 Jun 2024 14:26:16 -0400 Subject: [Histonet] Cloudy cornea after Hartmann's fixative Message-ID: Hartmann's fixative, so-called, got this name when Dr. William H. Hartmann introduced it in the mid-1960s at Johns Hopkins hospital, to fix tissue fo demonstrate nuclear detail, specifically Barr (sex chromatin) bodies, as directed in Moore and Barr's procedures for staining sex chromatin bodies, published a little before that time. Moore and Barr referred to it as "modified Davidson's fixative". Bill Hartmann's name stuck to it, though it really had nothing to do with him (as he hastened to point out). Bill Hartmann was one of my clinical teachers in surgical pathology at JHH at that time, so I was there for the occasion. He went on to Vanderbilt and other institutions. He died in 2016 at 85. John Kiernan knows more about Davidson's fixative. Probably Davidson never published it. Bob Richmond retired pathologist in Maryville TN From plucas at biopath.org Fri Jun 28 14:38:25 2024 From: plucas at biopath.org (Paula) Date: Fri, 28 Jun 2024 12:38:25 -0700 Subject: [Histonet] Group Purchasing Organizations Message-ID: <003401dac992$bf67a5a0$3e36f0e0$@biopath.org> Hello, I recently had a meeting with our ThermoFisher Scientific account manager, and she was asking me if we would be interested in joining a group purchasing organization (GPO) for our laboratory supplies. Can anyone comment on this subject whether it's worth it or not, if it's a good idea or not? I'm trying to consider the advantages and disadvantages of joining. Thank you in advance, Paula