From jmacdonald at mtsac.edu Tue Jan 2 17:24:48 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 2 Jan 2024 23:24:48 +0000 Subject: [Histonet] Positions for KP Chino Hills Histology In-Reply-To: References: Message-ID: We have three positions that have gone external for KP Chino Hills Histology. Our On-Call positions (per diem) will support staffing LOAs, vacations, and special projects. The hours and schedules may vary depending on the staffing and operational needs. We anticipate growth this year and look forward to building our staffing pool. Jesse Preciado, the Assistant Director of North Holly Histology, may also need potential applicants. * 1 On-Call Histology Technician - Must be HT/HTL certified with experience in embedding and microtomy. * 2 On-Call Laboratory Assistant II - Must have completed college-level general biology, chemistry, human anatomy, and mathematics courses or one year of Histology laboratory experience. Anyone interested can use the link below to apply. https://www.kaiserpermanentejobs.org/ From thisisann at aol.com Wed Jan 3 12:51:42 2024 From: thisisann at aol.com (Ann Specian) Date: Wed, 3 Jan 2024 18:51:42 +0000 (UTC) Subject: [Histonet] Embedding References: <1400990064.6325841.1704307902280.ref@mail.yahoo.com> Message-ID: <1400990064.6325841.1704307902280@mail.yahoo.com> Can anyone let me know if CAP, New York State or ISO have any required credentials for someone to be able to embed human tissue? Sent from the all new AOL app for iOS From jaylundgren at gmail.com Wed Jan 3 13:10:41 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 3 Jan 2024 13:10:41 -0600 Subject: [Histonet] Embedding In-Reply-To: <1400990064.6325841.1704307902280@mail.yahoo.com> References: <1400990064.6325841.1704307902280.ref@mail.yahoo.com> <1400990064.6325841.1704307902280@mail.yahoo.com> Message-ID: My lab uses trained lemurs, so I certainly hope not. Virus-free.www.avast.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Wed, Jan 3, 2024 at 12:51?PM Ann Specian via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Can anyone let me know if CAP, New York State or ISO have any required > credentials for someone to be able to embed human tissue? > > > Sent from the all new AOL app for iOS > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From relia1 at earthlink.net Mon Jan 15 11:44:26 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 15 Jan 2024 12:44:26 -0500 Subject: [Histonet] RELIA HOT RESEARCH Position. Message-ID: <018b01da47da$7d63dc30$782b9490$@earthlink.net> Hi Histonetters/Histopeeps! I hope this is the start to a great week for you! I want to give you a heads up on a few things. First! I am here to help if you need a free resume tune up. And here is a VERY hot Job Alert: RELIA HOT RESEARCH Job Alert!! Hi Histopeeps! I have an amazing research opportunity to share. My client is located in Eastern PA You will be working on a leading edge research team ready to make an amazing contribution to healthcare! This is a Full Time Permanent Day shift M-F Position. Strong routine histology and IHC skills are needed. You can RUN this LAB! My client offers a VERY competitive compensation package including relocation assistance. Yeah it's REALLY cold there now but in a few months it will be GORGEOUS! For more info contact me. PM/DM or email: relia1 at earthlink.net or cell/text 407-353-5070. Have a great day!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Tue Jan 16 12:28:29 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 16 Jan 2024 13:28:29 -0500 Subject: [Histonet] It's Transformation Tuesday - Career Edition! Even if you aren't looking for a job YOU want to READ this! Message-ID: <000401da48a9$ce7aacf0$6b7006d0$@earthlink.net> Hi Histonetters/Histopeeps! It's Transformation Tuesday - Career Edition! I can help you transform your career. It can be baby steps like: * Joining my group on Facebook: www.facebook.com/groups/histotechnologists * Career Coaching to maximize success at your current position. * A Resume Tune-up to see where you are in your career. * A "Salary Check" - Are you where you SHOULD be in compensation? OR We can take a more active approach: * Strategizing and implementing a search based on YOUR needs. * Interview Coaching * Salary Negotiations I will help you with EVERYTHING you need for a Career Transformation! All of my services are FREE to you as a service to the histology community! All you have to do is contact me! You can email: relia1 at earthlink.net or cell/text 407-353-5070. I have some pretty exciting opportunities that I am currently working on: Take a look and let me know what you think! * Is there something on this list you want to take a look at? * Let me know and I will fill you in on all of the details! * Is there something on this list that is "close" to what interests you? * Let me know what is right and what is not and let me find the 100% right one for you! I have opportunities in: * Research * QA/QC * Diagnostics * Mohs * Leadership Opportunities that I represent are with the "Cream of the Crop" of Histology Employers! And GUESS WHAT? My clients know YOU are also the "Cream of the Crop" because I only represent the BEST and BRIGHTEST! That's YOU!! All of the Positions I Represent Are: * Full time and Permanent * Offer excellent compensation packages * Locations throughout the U.S. TELL ME Where you want to be! I am looking forward to hearing from you!! Call/Text 407-353-5070 email: relia1 at earthlink.net Have a Fantastic Day!!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From bcooper at chla.usc.edu Tue Jan 16 15:18:42 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 16 Jan 2024 21:18:42 +0000 Subject: [Histonet] We were introduced to an awesome new product! Message-ID: <751573245129415ea7cfecd93d734d30@chla.usc.edu> Good afternoon HistoNet! You know that product that many of us use to skim our flotation baths in between cutting blocks? You know, those individual sheets in the green box? Well, for years now, I for one have been frustrated when say, a white-pages sized chunk of them come out when I go to grab one! (I know, I dated myself there). Or how the next sheet will retract inside the box, and then you have to surgically extract it to get on with it. Or how this product tears, and you're forced to skim your bath with only a fragment? It's been in every lab I've ever worked in! If you get a chance to try them, Tech-Wipe specialty task wipers are such an amazing upgrade! We have no financial gain here, I'd never even heard of this product until a few weeks ago. None of the issues I mentioned above have happened! Every one of our crew has told me how much they love this new product! These have a little bit of elasticity to them, and that seemingly prevents tears. And they only come out one at a time! Ask your consumables rep if they carry it! I never thought I'd write an email about skimming a waterbath; it's such a mundane task we've done millions of times as Histotechs. But here you go! Happy Tuesday! Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From relia1 at earthlink.net Thu Jan 18 12:00:33 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 18 Jan 2024 13:00:33 -0500 Subject: [Histonet] ICYMI!!!! RELIA HOT Research Job ALERT!! Message-ID: <01d901da4a38$3c162840$b44278c0$@earthlink.net> Hi Histonetters! I hope you are having a great day! ICYMI: This is a RELIA Exclusive!!! Here is the info: Exciting opportunity with GROWING Lab in Harrisburg, PA! Take a leadership role in this leading edge research lab. Get involved in exciting projects! Use or learn digital pathology! This is a full time permanent day shift position. My client offers excellent compensation, benefits and GROWTH! Relo is available as well. Work authorization is required for this position. For more information please contact Pam Barker at mailto:relia1 at earthlink.net OR cell/text 407-353-5070 ?#ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From kdean70 at hotmail.com Fri Jan 19 13:04:16 2024 From: kdean70 at hotmail.com (Ken M) Date: Fri, 19 Jan 2024 19:04:16 +0000 Subject: [Histonet] Aspergillus Message-ID: Can anyone provide me with Aspergillus tissue embedded blocks? Prefer good quality naturally occurring but will accept good quality home grown. We usually use Placenta, but the tissue doesn't matter much. Will buy or trade. Kdean70 at hotmail.com From TNMayer at mdanderson.org Mon Jan 22 10:18:04 2024 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Mon, 22 Jan 2024 16:18:04 +0000 Subject: [Histonet] Aspergillus In-Reply-To: References: Message-ID: Hi Ken, Try mushrooms. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Asst Professor/ Assoc Program Director HTL Program UTMDACC tnmayer at mdanderson.edu off cell: 832-710-1837 off: 713-563-3481 Today's Topics: 1. Aspergillus (Ken M) ---------------------------------------------------------------------- Message: 1 Date: Fri, 19 Jan 2024 19:04:16 +0000 From: Ken M To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Aspergillus Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone provide me with Aspergillus tissue embedded blocks? Prefer good quality naturally occurring but will accept good quality home grown. We usually use Placenta, but the tissue doesn't matter much. Will buy or trade. Kdean70 at hotmail.com ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!jxtxshQXGQFXTrfzFYP1NYCPgsH5ZamxgCrcudSmn5DnvDUxo7w8OQUxWhhr4-PF4L0rwrTQ6qNiZTkHsdSRNeaP5wY_VGWJ8DyfyTJOIec$ ------------------------------ End of Histonet Digest, Vol 242, Issue 6 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From Nancy.Schmitt at mercyhealth.com Mon Jan 22 14:50:00 2024 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Mon, 22 Jan 2024 20:50:00 +0000 Subject: [Histonet] prostate core/Decipher Message-ID: Hello! I am looking for input on how you are treating prostate core biopsy specimens that go to Veracyte for Decipher Prostate testing. We currently cut at 4 microns, 3 levels with 2 unstained at each level. Veracyte requests the block - which would have very little left - or 10 unstains at 5 microns. Are you adjusting you protocol up front for all prostate cases? Going down to 2 levels with additional unstains all at 5 microns? I appreciate your thoughts, Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Dubuque, IA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From jmacdonald at mtsac.edu Tue Jan 23 01:15:47 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 23 Jan 2024 07:15:47 +0000 Subject: [Histonet] California Society for Histotechnology Symposium Message-ID: The CSH is still looking for speakers for their annual education symposium. May 2-5 in Costa Mesa, California. Please contact me if you are interested in presenting a workshop. Thank you, Jennifer MacDonald https://www.californiahistology.org/home https://www.linkedin.com/search/results/all/?keywords=California%20Society%20for%20Histotechnology&origin=GLOBAL_SEARCH_HEADER&sid=q4V From relia1 at earthlink.net Tue Jan 23 11:18:43 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 23 Jan 2024 12:18:43 -0500 Subject: [Histonet] Ready for a Resume Tune-up? It's FREE of charge to ALL Histonetters!! Message-ID: <000f01da4e20$3814acd0$a83e0670$@earthlink.net> Hi Histonetters/Histopeeps! I hope this is a great week for you! I have been getting emails and calls from your fellow histopeeps asking if I am available to help them with their resumes. The Answer is YES! * Going for a promotion? * Prepping for a performance review? * Preparing for a presentation? * Transitioning from Travel to Permanent work? * Job Hunting? Whatever your reason, it is always a good idea to keep your resume updated. And... I will help you. Let me help you get your resume tuned up!! It's free of charge as a service to my Histopeeps!! Here's what you need to do: Reply to this email with I'm in and we can get started. Job Hunting? Here are some of my Hottest opportunities. All of these are permanent positions. Most are RELIA Exclusives All of my clients offer excellent compensation, benefits and most offer relocation/sign on bonuses!! I have some pretty exciting opportunities that I am currently working on: * Take a look and let me know what you think! * Is there something on this list you want to take a look at? * Let me know and I will fill you in on all of the details! * Is there something on this list that is "close" to what interests you? * Let me know what is right and what is not and let me find the 100% right one for you! I have opportunities in: * Research * QA/QC * Diagnostics * Mohs * Leadership Opportunities that I represent are with the "Cream of the Crop" of Histology Employers! And GUESS WHAT? My clients know YOU are also the "Cream of the Crop" because I only represent the BEST and BRIGHTEST! That's YOU!! All of the Positions I Represent Are: * Full time and Permanent * Offer excellent compensation packages * Locations throughout the U.S. TELL ME Where you want to be! I am looking forward to hearing from you!! Call/Text 407-353-5070 email: relia1 at earthlink.net Have a Fantastic Day!!! I look forward to assisting you!! I would like to invite you to join my Facebook group: www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail:relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histology #histologylab #histologyjobs #histologyiscool Follow my hashtags and make your day great and your career greater!! From relia1 at earthlink.net Wed Jan 24 11:48:27 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 24 Jan 2024 12:48:27 -0500 Subject: [Histonet] RELIA HOT Job Alert - Central Coast of California! Message-ID: <000701da4eed$9888d190$c99a74b0$@earthlink.net> Hi Histonetters! I hope you are having a great day. I have an exciting new opportunity to share with you! RELIA HOT Job Alert!! Great Wine Fresh Produce Beautiful Weather! I have a RELIA EXCLUSIVE opportunity on the Central Coast of California. Full time PERM M-F Days!!! Excellent Compensation and Fantastic Team to work with! ASCP HT or HTL and CLIA Qualified to gross is required for this position. For more information contact ME! Pam Barker! email relia1 at earthlink.net or cell/text 407-353-5070. #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From chak_bou at yahoo.com Wed Jan 24 16:34:15 2024 From: chak_bou at yahoo.com (Chakib Boussahmain) Date: Wed, 24 Jan 2024 22:34:15 +0000 (UTC) Subject: [Histonet] Bone samples References: <148232915.285398.1706135655260.ref@mail.yahoo.com> Message-ID: <148232915.285398.1706135655260@mail.yahoo.com> Hi guys, ?I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations. Thank you in advance for your help! Chakib From bcooper at chla.usc.edu Wed Jan 24 17:15:36 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 24 Jan 2024 23:15:36 +0000 Subject: [Histonet] Bone samples In-Reply-To: <148232915.285398.1706135655260@mail.yahoo.com> References: <148232915.285398.1706135655260.ref@mail.yahoo.com> <148232915.285398.1706135655260@mail.yahoo.com> Message-ID: Embed the bones diagonally in your molds if you're able (size depending) as this will allow for the greatest amount of paraffin support. Trim them very slowly, and if need be, place the blocks into the same "slow decal" solution for additional amount of time sufficient to enable better sectioning. Start checking in half hour or 45 minute intervals; rinse the blocks well in running water and attempt to section. If they're not ready, back into decal solution they go. I feel that very wet ice helps to facilitate sectioning better than ice that is drier and fresh out of the freezer. Just be sure to blot the face of the block with gauze before attempting to cut. There's my two cents! Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Chakib Boussahmain via Histonet Sent: Wednesday, January 24, 2024 2:34 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Bone samples (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Hi guys, ?I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations. Thank you in advance for your help! Chakib _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1oOSMT1FuuHaA4EXxjY3i9nIAlLf91ZjdN5e2afysmGGJYW7stpUWSe1_bubEbbdKpoHohJt-UQ7lkDBZ_BwXbYaFsVfbmyQtwDLG4qorqorzByzCfrpkWZnOwj1ZW6oWKhNoWYgB3KojnS_P3jfgs8QItdqAV5QGAqwEewtholWiGRWxd-ggJXBstwd3TKwVx9EdGpjtjB5T3-MrhwB6gYGfUkRWiSu2yt6PL7FbWYjl11MtUyGiXP6EcnRSb4fQZzykJavCYeY0XmEMR6pe-rxP8W-MsmYb7NizNBFhqYFhAbuqVDhn5joQziAtv9KSW6AAMi1oI6aQW2ncTolPPVcXljMP586zzPK9P-9m9eF_j2JwX1WZjXV7N5Du2QCgAl5Rsz3xpqP0b9vzEXZqZ021FHX5HNuoA0X0iKq9tyKn6gc_8OwLotyO2g4bQTZa_FQuxStxGkGG1wOCjwntTrbvdllnoHTF4aKGZMFPiA0/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From idimenstein at hotmail.com Thu Jan 25 21:57:08 2024 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Fri, 26 Jan 2024 03:57:08 +0000 Subject: [Histonet] Histonet Digest, Vol 242, Issue 10 In-Reply-To: References: Message-ID: I would like to add a couple cents to Brian's suggestions, especially how to embed the gross section. The "theory" in presented in my book Izak Dimenstein Grossing Bones: Principles, Technique, and Instruments. It depends on the level of hardness of the bone after decalcification or in the case of undemineralized bone section. Izak ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, January 25, 2024 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 242, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7C%7Cdc6c1c914a754597635808dc1dcf76b6%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C638418024131379854%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=mn6kBIQ%2B9xch9nT77gZUzVG5Cm6b8gfU9r%2Fvte%2BgPFQ%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From gu.lang at gmx.at Fri Jan 26 02:31:43 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 26 Jan 2024 09:31:43 +0100 Subject: [Histonet] Bone samples In-Reply-To: <148232915.285398.1706135655260@mail.yahoo.com> References: <148232915.285398.1706135655260.ref@mail.yahoo.com> <148232915.285398.1706135655260@mail.yahoo.com> Message-ID: <001701da5032$1828e0d0$487aa270$@gmx.at> Hi, In my opinion the hardness of the decalcified blocks is often rather due to the paraffin-processing than the residual calcium. Especially when the tissue is decalcified really long. The hardness comes from the dehydration and "cooking" of collagen fibers. So additional decal will not help reducing the calcium, but helps to reintroduce water into the collagen-grid. And this is helpful for softening and cutting. For myself, I often scratch the paraffin on the blocksurface away to face the bone directly to the water. Then I let them "swim" on my waterbath, until the surface is turned rather milky. After cooling again I cut in very very small steps to trim the surface. Sometimes it needs repeated swimming and cooling (and patience) to get a rather acceptable section. It is advantageous to pick them up on adhesive slides and let them dry in an 60?C oven to get rid of any residual water under the section. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: Chakib Boussahmain via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 24. J?nner 2024 23:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Bone samples Hi guys, I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations. Thank you in advance for your help! Chakib _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From simmca at UPMC.EDU Fri Jan 26 09:35:11 2024 From: simmca at UPMC.EDU (Simmons, Christopher) Date: Fri, 26 Jan 2024 15:35:11 +0000 Subject: [Histonet] Bone samples In-Reply-To: <001701da5032$1828e0d0$487aa270$@gmx.at> References: <148232915.285398.1706135655260.ref@mail.yahoo.com> <148232915.285398.1706135655260@mail.yahoo.com> <001701da5032$1828e0d0$487aa270$@gmx.at> Message-ID: Ion Exchange Decal containers will change your experience forever. -----Original Message----- From: Gudrun Lang via Histonet Sent: Friday, January 26, 2024 3:32 AM To: 'Chakib Boussahmain' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Bone samples Hi, In my opinion the hardness of the decalcified blocks is often rather due to the paraffin-processing than the residual calcium. Especially when the tissue is decalcified really long. The hardness comes from the dehydration and "cooking" of collagen fibers. So additional decal will not help reducing the calcium, but helps to reintroduce water into the collagen-grid. And this is helpful for softening and cutting. For myself, I often scratch the paraffin on the blocksurface away to face the bone directly to the water. Then I let them "swim" on my waterbath, until the surface is turned rather milky. After cooling again I cut in very very small steps to trim the surface. Sometimes it needs repeated swimming and cooling (and patience) to get a rather acceptable section. It is advantageous to pick them up on adhesive slides and let them dry in an 60?C oven to get rid of any residual water under the section. Hope this helps Gudrun -----Urspr?ngliche Nachricht----- Von: Chakib Boussahmain via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 24. J?nner 2024 23:34 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Bone samples Hi guys, I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations. Thank you in advance for your help! Chakib _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!NHLzug!Jh6cF0ugJABQPV4zi-V-Y0UPbZ4bUroLeBNYiAxwTdz6uiNtFvuO5RlsEtEwCqK5OzDLLv9GdeiF4tdq52dWnRiMVbxh3w$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!NHLzug!Jh6cF0ugJABQPV4zi-V-Y0UPbZ4bUroLeBNYiAxwTdz6uiNtFvuO5RlsEtEwCqK5OzDLLv9GdeiF4tdq52dWnRiMVbxh3w$ From relia1 at earthlink.net Tue Jan 30 11:35:34 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 30 Jan 2024 12:35:34 -0500 Subject: [Histonet] Hi Histonetters!! It's Tip Tuesday! Message-ID: <004901da53a2$bb65f6c0$3231e440$@earthlink.net> Hi Histonetters !! It's Tip Tuesday. And I have a tip for you! Using a recruiter for your job search can make your job search more successful! How you ask? There are many ways but here is one of the most critical! Because they can help you negotiate a better salary than you can on your own! And that's just one way... Follow me on Social Media (FB, LI, IG) for more tips on working with recruiters! "PamBarkerRELIASolutions" or "PamatRELIA" If you are currently conducting or contemplating a job search, I can help. Just shoot me an email to relia1 at earthlink.net or call text 407-353-5070 Histonetters, I have some pretty exciting opportunities that I am currently working on: * Take a look and let me know what you think! * Is there something on this list you want to take a look at? * Let me know and I will fill you in on all of the details! * Is there something on this list that is "close" to what interests you? * Let me know what is right and what is not and let me find the 100% right one for you! 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Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From afoshey at mcw.edu Tue Jan 30 14:14:20 2024 From: afoshey at mcw.edu (Foshey, Annette) Date: Tue, 30 Jan 2024 20:14:20 +0000 Subject: [Histonet] Tissue falling off slides Message-ID: I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the slides the tissue still lifts off the slides usually it is where there is tumor. Any suggestions would be greatly appreciated. Kindest Regards, Annette Foshey, HT Histology Technologist, Precision Medicine Laboratory (PML) Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 From rcartun at sbcglobal.net Wed Jan 31 09:29:16 2024 From: rcartun at sbcglobal.net (richard cartun) Date: Wed, 31 Jan 2024 15:29:16 +0000 (UTC) Subject: [Histonet] Tissue falling off slides In-Reply-To: References: Message-ID: <599549743.3503003.1706714956087@mail.yahoo.com> Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight.? On some of the slides the tissue still lifts off the slides usually it is where there is tumor.? Any suggestions would be greatly appreciated. Kindest Regards, Annette Foshey, HT Histology Technologist, Precision Medicine Laboratory (PML) Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Jan 31 12:26:36 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 31 Jan 2024 13:26:36 -0500 Subject: [Histonet] ICYMI Here is some of the WORST Career Advice YOU will ever see! And Exciting NEW Opportunities - RELIA Exclusives!!! Message-ID: <026301da5473$07068150$151383f0$@earthlink.net> Hi Histonetters!! I hope you?re having a great day! I saw this article online. I would usually share with my recruiter group and once I read it, I KNEW I had to share it with you! ICYMI Here is some of the WORST Career Advice YOU will ever see! (The Don'ts!) https://www.businessinsider.com/heres-the-worst-career-advice-youve-ever-rec eived-2016-6 I also have some exciting opportunities. Right NOW, and new positions are coming in daily! Even if you aren?t looking maybe, you know someone who might be. And things change so quickly you never know when you might be. Ready For A Change. So, if you have a second, please take a look at my newest and best opportunities. Please drop me a line if you are interested in any of them or even if you are looking for something else somewhere else. I Will Help You. 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Barker President/Senior Recruiting Specialist-Histology RELIA? Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Wed Jan 31 12:32:59 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 31 Jan 2024 13:32:59 -0500 Subject: [Histonet] IOops! Something went wrong - Re-Post with link! Histonet] ICYMI Here is some of the WORST Career Advice YOU will ever see! And Exciting NEW Opportunities - RELIA Exclusives!!! Message-ID: <027801da5473$eb931c70$c2b95550$@earthlink.net> Hi Histonetters!! I hope you?re having a great day! I saw this article online. I would usually share with my recruiter group and once I read it, I KNEW I had to share it with you! ICYMI Here is some of the WORST Career Advice YOU will ever see! (The Don'ts!) https://www.businessinsider.com/heres-the-worst-career-advice-youve-ever-re ceived-2016-6 I also have some exciting opportunities. Right NOW, and new positions are coming in daily! Even if you aren?t looking maybe, you know someone who might be. And things change so quickly you never know when you might be. Ready For A Change. So, if you have a second, please take a look at my newest and best opportunities. Please drop me a line if you are interested in any of them or even if you are looking for something else somewhere else. I Will Help You. I Have Exciting Opportunities In: * Kentucky * Florida * California * Pennsylvania * Alabama * The Carolinas With New Positions Coming In For: * Virginia * Tennessee * Texas * Louisiana * Ohio * Indiana All of my clients offer excellent compensation, benefits. and some offer relocation assistance and or sign on bonuses. All of these jobs are full time & permanent. Some are RELIA EXCLUSIVES and Some are Brand NEW LABS! NEW POSITIONS ARE COMING IN DAILY NATIONWIDE!!! So, Drop me a line and let me know where you want to go!! If you or anyone you know is interested in hearing more about any of these opportunities, please contact me. Remember if I place someone you refer to me you will earn a referral bonus!!! I can be reached anytime on my cell/text at 407-353-5070 or toll free at 866-607-3542 or e-mail me at mailto:relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker President/Senior Recruiting Specialist-Histology RELIA? Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: mailto:relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals http://www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From kdean70 at hotmail.com Wed Jan 31 17:06:40 2024 From: kdean70 at hotmail.com (Ken M) Date: Wed, 31 Jan 2024 23:06:40 +0000 Subject: [Histonet] Tissue falling off slides In-Reply-To: <599549743.3503003.1706714956087@mail.yahoo.com> References: <599549743.3503003.1706714956087@mail.yahoo.com> Message-ID: 1) Try tap water 2) Try hydrophilic IHC slides, (I will send you samples for free) Ken Kdean70 at hotmail.com -----Original Message----- From: richard cartun via Histonet Sent: Wednesday, January 31, 2024 10:29 AM To: histonet at lists.utsouthwestern.edu; Foshey, Annette Subject: Re: [Histonet] Tissue falling off slides Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the slides the tissue still lifts off the slides usually it is where there is tumor. Any suggestions would be greatly appreciated. Kindest Regards, Annette Foshey, HT Histology Technologist, Precision Medicine Laboratory (PML) Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgonzalez at origene.com Wed Jan 31 20:58:58 2024 From: rgonzalez at origene.com (Rachel Gonzalez) Date: Thu, 1 Feb 2024 02:58:58 +0000 Subject: [Histonet] Tissue falling off slides In-Reply-To: References: <599549743.3503003.1706714956087@mail.yahoo.com> Message-ID: Hi I used to have that problem. A senior histologist from BioCare recommended to air dry the slides overnight and then bake them. I have not had the problem since. Rachel From: Ken M via Histonet Sent: Wednesday, January 31, 2024 6:07 PM To: richard cartun ; histonet at lists.utsouthwestern.edu; Foshey, Annette Subject: Re: [Histonet] Tissue falling off slides 1) Try tap water 2) Try hydrophilic IHC slides, (I will send you samples for free) Ken Kdean70 at hotmail.com -----Original Message----- From: richard cartun via Histonet > Sent: Wednesday, January 31, 2024 10:29 AM To: histonet at lists.utsouthwestern.edu; Foshey, Annette > Subject: Re: [Histonet] Tissue falling off slides Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet > wrote: I work with whole mount prostate blocks that I cut and stain for IHC manually. I use superfrost plus slides that I purchase from the Brain Research Laboratories. I airdry the slides after sectioning and then they are placed in a rack and dried in a 60 degree oven overnight. On some of the slides the tissue still lifts off the slides usually it is where there is tumor. Any suggestions would be greatly appreciated. Kindest Regards, Annette Foshey, HT Histology Technologist, Precision Medicine Laboratory (PML) Phone: (414) 955-7537 | E-mail: afoshey at mcw.edu> Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. 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