From Milton.Gomez at nyulangone.org Thu Feb 1 18:40:05 2024 From: Milton.Gomez at nyulangone.org (Gomez, Milton) Date: Fri, 2 Feb 2024 00:40:05 +0000 Subject: [Histonet] Laboratory metrics Message-ID: <9c9afd5e92bd49a9ba62137bc3b8d160@nyulangone.org> Dear Histonetters, What metrics do you use to monitor productivity, oversee QC efforts and improve TAT? Thanks, MG From someperson43 at yahoo.com Fri Feb 2 21:36:42 2024 From: someperson43 at yahoo.com (Verizon wireless) Date: Sat, 3 Feb 2024 03:36:42 +0000 (UTC) Subject: [Histonet] Processing artifact - delayed start In-Reply-To: References: Message-ID: <1948151075.4695153.1706931402608@mail.yahoo.com> Dear Histonet Members, We have terrible processing artifact if tissue sits in the formalin-filled retort (at ambient temperature) for too long (more than 10-12 hours) before a delayed process starts. The longer the wait, the worse it looks. We have Tissue Tek VIP 5 processors, and we process luminal gastrointestinal biopsies exclusively. I've attached some photomicrographs of problem cases on the Histonet Images website (with the same topic title).? This artifact typically affects a few specimens per day (~2% or less), even though everything is done on the same processor; it may affect all tissue portions in a cassette or only some of the tissue in that cassette. Some tissue portions may only have the artifact on one half with the other half looking perfectly fine. The techs sometimes note the tissue feeling "crunchy" at the time of embedding and / or at the time of microtomy. These tissues tend to suffer greater chatter artifact and have trouble sticking to the slides. The sections look just as bad on recuts as the originals. Re-processing does not seem to help at all.? If the cassettes sit in formalin in a container outside of the processor for days before the processor is loaded (with subsequent immediate start), things look perfectly fine. When we have staff around to start the processor immediately upon loading cassettes and empty immediately upon completion, the tissue looks perfectly fine. Our current processing program is as follows: 1. 10% Formalin, 30 minutes, ambient temp, p/v on 2. 10% Formalin, 30 minutes, ambient temp, p/v on 3. 65% Alcohol, 10 minutes, ambient temp, p/v on4. 80% Alcohol, 10 minutes, ambient temp, p/v on5. 95% Alcohol, 7 minutes, ambient temp, p/v on6. 95% Alcohol, 7 minutes, ambient temp, p/v on 7. 100% Alcohol, 10 minutes, ambient temp, p/v on 8. 100% Alcohol, 10 minutes, ambient temp, p/v on 9. Xylene, 15 minutes, ambient temp, p/v on 10. Xylene, 15 minutes, ambient temp, p/v on 11. Paraffin, 10 minutes, 60 degrees C, p/v on12. Paraffin, 10 minutes, 60 degrees C, p/v on13. Paraffin, 15 minutes, 60 degrees C, p/v on14. Paraffin, 15 minutes, 60 degrees C, p/v on Obviously there are times we absolutely need to use delayed start. I would greatly appreciate guidance, and I'll be happy to provide any other details that might be useful. Sincerely, Brian Quigley MDLaboratory Director of a GI Pathology Laboratory From afhenwood at outlook.com Sat Feb 3 01:06:24 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Sat, 3 Feb 2024 07:06:24 +0000 Subject: [Histonet] Processing artifact - delayed start In-Reply-To: <1948151075.4695153.1706931402608@mail.yahoo.com> References: <1948151075.4695153.1706931402608@mail.yahoo.com> Message-ID: I would check the level of the formalin after it has been pumped into the retort. I will wait till the pics are posted Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Verizon wireless via Histonet Sent: Saturday, February 3, 2024 2:36:42 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing artifact - delayed start Dear Histonet Members, We have terrible processing artifact if tissue sits in the formalin-filled retort (at ambient temperature) for too long (more than 10-12 hours) before a delayed process starts. The longer the wait, the worse it looks. We have Tissue Tek VIP 5 processors, and we process luminal gastrointestinal biopsies exclusively. I've attached some photomicrographs of problem cases on the Histonet Images website (with the same topic title). This artifact typically affects a few specimens per day (~2% or less), even though everything is done on the same processor; it may affect all tissue portions in a cassette or only some of the tissue in that cassette. Some tissue portions may only have the artifact on one half with the other half looking perfectly fine. The techs sometimes note the tissue feeling "crunchy" at the time of embedding and / or at the time of microtomy. These tissues tend to suffer greater chatter artifact and have trouble sticking to the slides. The sections look just as bad on recuts as the originals. Re-processing does not seem to help at all. If the cassettes sit in formalin in a container outside of the processor for days before the processor is loaded (with subsequent immediate start), things look perfectly fine. When we have staff around to start the processor immediately upon loading cassettes and empty immediately upon completion, the tissue looks perfectly fine. Our current processing program is as follows: 1. 10% Formalin, 30 minutes, ambient temp, p/v on 2. 10% Formalin, 30 minutes, ambient temp, p/v on 3. 65% Alcohol, 10 minutes, ambient temp, p/v on4. 80% Alcohol, 10 minutes, ambient temp, p/v on5. 95% Alcohol, 7 minutes, ambient temp, p/v on6. 95% Alcohol, 7 minutes, ambient temp, p/v on 7. 100% Alcohol, 10 minutes, ambient temp, p/v on 8. 100% Alcohol, 10 minutes, ambient temp, p/v on 9. Xylene, 15 minutes, ambient temp, p/v on 10. Xylene, 15 minutes, ambient temp, p/v on 11. Paraffin, 10 minutes, 60 degrees C, p/v on12. Paraffin, 10 minutes, 60 degrees C, p/v on13. Paraffin, 15 minutes, 60 degrees C, p/v on14. Paraffin, 15 minutes, 60 degrees C, p/v on Obviously there are times we absolutely need to use delayed start. I would greatly appreciate guidance, and I'll be happy to provide any other details that might be useful. Sincerely, Brian Quigley MDLaboratory Director of a GI Pathology Laboratory _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Bacon at baystatehealth.org Mon Feb 5 07:07:41 2024 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Mon, 5 Feb 2024 13:07:41 +0000 Subject: [Histonet] Processing artifact - delayed start In-Reply-To: <1948151075.4695153.1706931402608@mail.yahoo.com> References: <1948151075.4695153.1706931402608@mail.yahoo.com> Message-ID: We have had 2 reasons why we saw processing issues like this: 1. I recently found out on our VIP 5 they did not turn the level sensors on during install. These sensors are known to error so often, Sakura tells technicians to set the default to off. All the processor can sense is pressure and time. So it may be that the formalin is not filling all the way. You are noticing this on delayed runs, but on those runs are you often stacking trays? If so, isolate the cases in the upper trays and review. 2. We found a clogged line. This happens with the formalin lines if you don?t do the hot water flush often enough. This can be an even bigger issue if you recycle and re-buffer your formalin (we do not). Good luck! Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center -----Original Message----- From: Verizon wireless Sent: Friday, February 2, 2024 10:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing artifact - delayed start Dear Histonet Members, We have terrible processing artifact if tissue sits in the formalin-filled retort (at ambient temperature) for too long (more than 10-12 hours) before a delayed process starts. The longer the wait, the worse it looks. We have Tissue Tek VIP 5 processors, and we process luminal gastrointestinal biopsies exclusively. I've attached some photomicrographs of problem cases on the Histonet Images website (with the same topic title). This artifact typically affects a few specimens per day (~2% or less), even though everything is done on the same processor; it may affect all tissue portions in a cassette or only some of the tissue in that cassette. Some tissue portions may only have the artifact on one half with the other half looking perfectly fine. The techs sometimes note the tissue feeling "crunchy" at the time of embedding and / or at the time of microtomy. These tissues tend to suffer greater chatter artifact and have trouble sticking to the slides. The sections look just as bad on recuts as the originals. Re-processing does not seem to help at all. If the cassettes sit in formalin in a container outside of the processor for days before the processor is loaded (with subsequent immediate start), things look perfectly fine. When we have staff around to start the processor immediately upon loading cassettes and empty immediately upon completion, the tissue looks perfectly fine. Our current processing program is as follows: 1. 10% Formalin, 30 minutes, ambient temp, p/v on 2. 10% Formalin, 30 minutes, ambient temp, p/v on 3. 65% Alcohol, 10 minutes, ambient temp, p/v on4. 80% Alcohol, 10 minutes, ambient temp, p/v on5. 95% Alcohol, 7 minutes, ambient temp, p/v on6. 95% Alcohol, 7 minutes, ambient temp, p/v on 7. 100% Alcohol, 10 minutes, ambient temp, p/v on 8. 100% Alcohol, 10 minutes, ambient temp, p/v on 9. Xylene, 15 minutes, ambient temp, p/v on 10. Xylene, 15 minutes, ambient temp, p/v on 11. Paraffin, 10 minutes, 60 degrees C, p/v on12. Paraffin, 10 minutes, 60 degrees C, p/v on13. Paraffin, 15 minutes, 60 degrees C, p/v on14. Paraffin, 15 minutes, 60 degrees C, p/v on Obviously there are times we absolutely need to use delayed start. I would greatly appreciate guidance, and I'll be happy to provide any other details that might be useful. Sincerely, Brian Quigley MDLaboratory Director of a GI Pathology Laboratory From ruppert.amysue at marshfieldclinic.org Wed Feb 7 01:28:06 2024 From: ruppert.amysue at marshfieldclinic.org (Ruppert, Amysue) Date: Wed, 7 Feb 2024 07:28:06 +0000 Subject: [Histonet] processing artifact - delayed start In-Reply-To: References: Message-ID: I am wondering if you are getting air bubbles/pockets in the cassettes themselves. Are you using mesh cassettes? Are you loading the trays with cassettes into an empty retort and then starting the delayed program? If so, air pockets could be forming in the cassette and around the tissue pieces. Since there is no p/v cycle running yet, those air pockets may be just sitting there and the exposed tissue pieces may be getting dried out. I suggest that once the retort is full and in ambient mode, open retort and move the cassette trays around a bit and see if air bubbles are rising from the cassettes. When the trays of cassettes sit on the bench in formalin waiting to go on the VIP, there may be enough movement when moving the container, etc, that any air pockets are disrupted and the tissues are not exposed to air pockets. good luck, amysue ruppert Marshfield Labs Histology ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Monday, February 5, 2024 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 243, Issue 4 CAUTION: This email originated from outside of the Marshfield Clinic Health System. Do not click links or open attachments unless you recognize the sender and know the content is safe. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!J7HzeEKFbK9hUUY!PGATEy43_xJX9a3K3Na-c7VKlFfxZ5567gwny4UONDtiVRyrbBI5aHBtxx6V0DTFWbIgFABz11BpPHqIM6HdmZ9BO2ad6vok37eGCo6_gU_9gOLs_lTLCQ$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Processing artifact - delayed start (Bacon, Charles) ---------------------------------------------------------------------- Message: 1 Date: Mon, 5 Feb 2024 13:07:41 +0000 From: "Bacon, Charles" To: Verizon wireless , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Processing artifact - delayed start Message-ID: Content-Type: text/plain; charset="utf-8" We have had 2 reasons why we saw processing issues like this: 1. I recently found out on our VIP 5 they did not turn the level sensors on during install. These sensors are known to error so often, Sakura tells technicians to set the default to off. All the processor can sense is pressure and time. So it may be that the formalin is not filling all the way. You are noticing this on delayed runs, but on those runs are you often stacking trays? If so, isolate the cases in the upper trays and review. 2. We found a clogged line. This happens with the formalin lines if you don?t do the hot water flush often enough. This can be an even bigger issue if you recycle and re-buffer your formalin (we do not). Good luck! Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center -----Original Message----- From: Verizon wireless Sent: Friday, February 2, 2024 10:37 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Processing artifact - delayed start Dear Histonet Members, We have terrible processing artifact if tissue sits in the formalin-filled retort (at ambient temperature) for too long (more than 10-12 hours) before a delayed process starts. The longer the wait, the worse it looks. We have Tissue Tek VIP 5 processors, and we process luminal gastrointestinal biopsies exclusively. I've attached some photomicrographs of problem cases on the Histonet Images website (with the same topic title). This artifact typically affects a few specimens per day (~2% or less), even though everything is done on the same processor; it may affect all tissue portions in a cassette or only some of the tissue in that cassette. Some tissue portions may only have the artifact on one half with the other half looking perfectly fine. The techs sometimes note the tissue feeling "crunchy" at the time of embedding and / or at the time of microtomy. These tissues tend to suffer greater chatter artifact and have trouble sticking to the slides. The sections look just as bad on recuts as the originals. Re-processing does not seem to help at all. If the cassettes sit in formalin in a container outside of the processor for days before the processor is loaded (with subsequent immediate start), things look perfectly fine. When we have staff around to start the processor immediately upon loading cassettes and empty immediately upon completion, the tissue looks perfectly fine. Our current processing program is as follows: 1. 10% Formalin, 30 minutes, ambient temp, p/v on 2. 10% Formalin, 30 minutes, ambient temp, p/v on 3. 65% Alcohol, 10 minutes, ambient temp, p/v on4. 80% Alcohol, 10 minutes, ambient temp, p/v on5. 95% Alcohol, 7 minutes, ambient temp, p/v on6. 95% Alcohol, 7 minutes, ambient temp, p/v on 7. 100% Alcohol, 10 minutes, ambient temp, p/v on 8. 100% Alcohol, 10 minutes, ambient temp, p/v on 9. Xylene, 15 minutes, ambient temp, p/v on 10. Xylene, 15 minutes, ambient temp, p/v on 11. Paraffin, 10 minutes, 60 degrees C, p/v on12. Paraffin, 10 minutes, 60 degrees C, p/v on13. Paraffin, 15 minutes, 60 degrees C, p/v on14. Paraffin, 15 minutes, 60 degrees C, p/v on Obviously there are times we absolutely need to use delayed start. I would greatly appreciate guidance, and I'll be happy to provide any other details that might be useful. Sincerely, Brian Quigley MDLaboratory Director of a GI Pathology Laboratory ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!J7HzeEKFbK9hUUY!PGATEy43_xJX9a3K3Na-c7VKlFfxZ5567gwny4UONDtiVRyrbBI5aHBtxx6V0DTFWbIgFABz11BpPHqIM6HdmZ9BO2ad6vok37eGCo6_gU_9gOLs_lTLCQ$ ------------------------------ End of Histonet Digest, Vol 243, Issue 4 **************************************** ______________________________________________________________________ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. From plucas at biopath.org Wed Feb 7 13:35:26 2024 From: plucas at biopath.org (Paula) Date: Wed, 7 Feb 2024 11:35:26 -0800 Subject: [Histonet] Marking Pen Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org> Hello, We have been using KP Marker Plus pens for cassettes. We have 2 Leica Processors and 1 VIP6 processor. The cassettes that go into the VIP6 are smeared and some are almost smeared off completely. The processors have the same solutions in them. If anyone can shed some light as to why there is a difference, and if anyone can recommend a better marking pen for us to try, I would appreciate the feedback. Thank you, Paula From Jessica.Piche at wtbyhosp.org Thu Feb 8 05:30:03 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 8 Feb 2024 11:30:03 +0000 Subject: [Histonet] Marking Pen In-Reply-To: <009e01da59fc$ce13b680$6a3b2380$@biopath.org> References: <009e01da59fc$ce13b680$6a3b2380$@biopath.org> Message-ID: Hi Paula, What is the first solution the cassettes go in when the processor starts? We use Statmark pens when our cassette printer isn't working, and they work well. The only time we have had issues was when we would hand write the cassettes and run them on our small biopsy run which skips the formalin. It seems like the marker ink needs to "fix" with the formalin. Sometimes they smear if they aren't dry enough before they go into formalin too. I hope you figure it out. Maybe see if you can get some samples of different pens and then run some empty cassettes and see what works best for you. Have a good day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: Paula via Histonet Sent: Wednesday, February 7, 2024 2:35 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Marking Pen [EXTERNAL MSG] Hello, We have been using KP Marker Plus pens for cassettes. We have 2 Leica Processors and 1 VIP6 processor. The cassettes that go into the VIP6 are smeared and some are almost smeared off completely. The processors have the same solutions in them. If anyone can shed some light as to why there is a difference, and if anyone can recommend a better marking pen for us to try, I would appreciate the feedback. Thank you, Paula _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D&reserved=0 From SThompson4 at sonichealthcareusa.com Thu Feb 8 11:19:28 2024 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Thu, 8 Feb 2024 17:19:28 +0000 Subject: [Histonet] Lead Histotechnologist Message-ID: Looking for the next step in your career? Sonic Healthcare USA has an opportunity for a Lead Histotechnologist. Quality is in our DNA -- is it in yours? Join our front line of #HealthcareHeroes! Our mission is to advance the health and wellbeing of our communities as a leader in clinical laboratory solutions. Location: Exeter, NH Days: Monday - Friday 9:00 PM - 5:30 AM Full-time: Benefit Eligible In this role, you will: Prepare histologic slides from human tissue sections for microscopic examination and diagnosis by Pathologist. * Exercises independent judgment in dealing with procedural and technical problems. * Examines slides and/or blocks to ensure tissue preparation is meeting laboratory requirements. * Trains or directs Laboratory Assistants and Histology Technicians engaged in laboratory testing and processing techniques. * Prepares sections of human tissue for microscopic examination and patient diagnosis, using techniques to gross (dissect tissue), embed (orient specimen in paraffin block), section (cut thin sections of tissue), stain (enhance contrast of tissue and highlight specific features of interest with routine hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass slides), from surgical procedures. * Performs recuts and additional stains including special and immunohistochemistry stains, as requested by a Pathologist. * Operates computerized laboratory equipment to fix, dehydrate, and infiltrate with wax, tissue specimens to be preserved for study by Pathologist. * May label requisitions, specimen containers, cassettes and/or slides and affixes coverslip to slides. * Maintains laboratory equipment and tracks all routine maintenance and quality controls performed. * Files, retrieves, and distributes blocks, slides, and pathology reports. * Operates, cleans, and sterilizes laboratory equipment, glassware, instruments, and workstation. * Disposes of hazardous chemical waste per regulatory guidelines. * Maintains strictest confidentiality. * Complies with all State, Federal, professional regulations as well as company and departmental rules, polices, and procedural manuals. * Adherence to CAP, CLIA, State Regulations, HIPAA, Safety and OSHA Regulations. All you need is: * High School diploma or equivalent required. Associates or Bachelors of Science degree and completion of histotechnology program required. Certification as a histotechnician (HT) or histotechnologist (HTL) by American Society of Clinical Pathology (ASCP) preferred. * State licensure, if applicable. * Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) * Completion of a Histology program accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year experience as a full-time Histology Technician Trainee and competent in the areas of fixation, processing, embedding, microtomy, grossing, special stains, immunohistochemistry, and lab operations. Company: Sonic Anatomic Pathology Please feel free to contact me: Stephanie Thompson - 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From kathleenbess at sbcglobal.net Thu Feb 8 13:29:39 2024 From: kathleenbess at sbcglobal.net (KATHLEEN FERNANDEZ) Date: Thu, 8 Feb 2024 13:29:39 -0600 Subject: [Histonet] Histonet Digest, Vol 243, Issue 6 In-Reply-To: References: Message-ID: <3F34A6AB-DFAB-4E64-94BB-14D64D7C239D@sbcglobal.net> Paula, We are using the KP?s at our lab too and have been noticing that they are smearing! We are having issues on our slides, since we hand write still. This just recently started happening. Maybe they changed their formula? Kathy Sent from my iPhone > On Feb 8, 2024, at 12:01?PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Marking Pen (Paula) > 2. Re: Marking Pen (Piche, Jessica) > 3. Lead Histotechnologist (Stephanie L. Thompson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 7 Feb 2024 11:35:26 -0800 > From: "Paula" > To: > Subject: [Histonet] Marking Pen > Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > > > We have been using KP Marker Plus pens for cassettes. We have 2 Leica > Processors and 1 VIP6 processor. The cassettes that go into the VIP6 are > smeared and some are almost smeared off completely. The processors have the > same solutions in them. > > > > If anyone can shed some light as to why there is a difference, and if anyone > can recommend a better marking pen for us to try, I would appreciate the > feedback. > > > > Thank you, > > Paula > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 Feb 2024 11:30:03 +0000 > From: "Piche, Jessica" > To: "histonet at lists.utsouthwestern.edu" > , Paula > Subject: Re: [Histonet] Marking Pen > Message-ID: > > > Content-Type: text/plain; charset="us-ascii" > > Hi Paula, > > What is the first solution the cassettes go in when the processor starts? We use Statmark pens when our cassette printer isn't working, and they work well. The only time we have had issues was when we would hand write the cassettes and run them on our small biopsy run which skips the formalin. It seems like the marker ink needs to "fix" with the formalin. Sometimes they smear if they aren't dry enough before they go into formalin too. I hope you figure it out. Maybe see if you can get some samples of different pens and then run some empty cassettes and see what works best for you. > > Have a good day. > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory > Histology Team Leader > 203-573-7167 > ________________________________ > From: Paula via Histonet > Sent: Wednesday, February 7, 2024 2:35 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Marking Pen > > [EXTERNAL MSG] > > Hello, > > > > We have been using KP Marker Plus pens for cassettes. We have 2 Leica > Processors and 1 VIP6 processor. The cassettes that go into the VIP6 are > smeared and some are almost smeared off completely. The processors have the > same solutions in them. > > > > If anyone can shed some light as to why there is a difference, and if anyone > can recommend a better marking pen for us to try, I would appreciate the > feedback. > > > > Thank you, > > Paula > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D&reserved=0 > > > ------------------------------ > > Message: 3 > Date: Thu, 8 Feb 2024 17:19:28 +0000 > From: "Stephanie L. Thompson" > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Lead Histotechnologist > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Looking for the next step in your career? > > Sonic Healthcare USA has an opportunity for a Lead Histotechnologist. > > Quality is in our DNA -- is it in yours? > > Join our front line of #HealthcareHeroes! Our mission is to advance the health and wellbeing of our communities as a leader in clinical laboratory solutions. > > Location: Exeter, NH > Days: Monday - Friday > 9:00 PM - 5:30 AM > Full-time: Benefit Eligible > > In this role, you will: > > Prepare histologic slides from human tissue sections for microscopic examination and diagnosis by Pathologist. > > * Exercises independent judgment in dealing with procedural and technical problems. > * Examines slides and/or blocks to ensure tissue preparation is meeting laboratory requirements. > * Trains or directs Laboratory Assistants and Histology Technicians engaged in laboratory testing and processing techniques. > * Prepares sections of human tissue for microscopic examination and patient diagnosis, using techniques to gross (dissect tissue), embed (orient specimen in paraffin block), section (cut thin sections of tissue), stain (enhance contrast of tissue and highlight specific features of interest with routine hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass slides), from surgical procedures. > * Performs recuts and additional stains including special and immunohistochemistry stains, as requested by a Pathologist. > * Operates computerized laboratory equipment to fix, dehydrate, and infiltrate with wax, tissue specimens to be preserved for study by Pathologist. > * May label requisitions, specimen containers, cassettes and/or slides and affixes coverslip to slides. > * Maintains laboratory equipment and tracks all routine maintenance and quality controls performed. > * Files, retrieves, and distributes blocks, slides, and pathology reports. > * Operates, cleans, and sterilizes laboratory equipment, glassware, instruments, and workstation. > * Disposes of hazardous chemical waste per regulatory guidelines. > * Maintains strictest confidentiality. > * Complies with all State, Federal, professional regulations as well as company and departmental rules, polices, and procedural manuals. > * Adherence to CAP, CLIA, State Regulations, HIPAA, Safety and OSHA Regulations. > > All you need is: > > * High School diploma or equivalent required. Associates or Bachelors of Science degree and completion of histotechnology program required. Certification as a histotechnician (HT) or histotechnologist (HTL) by American Society of Clinical Pathology (ASCP) preferred. > * State licensure, if applicable. > * Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) > * Completion of a Histology program accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year experience as a full-time Histology Technician Trainee and competent in the areas of fixation, processing, embedding, microtomy, grossing, special stains, immunohistochemistry, and lab operations. > > Company: > Sonic Anatomic Pathology > > Please feel free to contact me: > Stephanie Thompson - 210-428-1646 > > > > This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 243, Issue 6 > **************************************** From paula at excaliburpathology.com Thu Feb 8 13:49:47 2024 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 8 Feb 2024 19:49:47 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 243, Issue 6 In-Reply-To: <3F34A6AB-DFAB-4E64-94BB-14D64D7C239D@sbcglobal.net> References: <3F34A6AB-DFAB-4E64-94BB-14D64D7C239D@sbcglobal.net> Message-ID: <222021138.64537.1707421788546@mail.yahoo.com> When I need to handwrite on slides or cassettes, I use Sakura Pigma Micron archival ink pens. They come in different tip sizes and colors and are found at any office supply store. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, February 8, 2024 at 01:43:43 PM CST, KATHLEEN FERNANDEZ via Histonet wrote: Paula, We are using the KP?s at our lab too and have been noticing that they are smearing! We are having issues on our slides, since we hand write still. This just recently started happening. Maybe they changed their formula? Kathy Sent from my iPhone > On Feb 8, 2024, at 12:01?PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to >? ? histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >? ? http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to >? ? histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at >? ? histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > >? 1. Marking Pen (Paula) >? 2. Re: Marking Pen (Piche, Jessica) >? 3. Lead Histotechnologist (Stephanie L. Thompson) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 7 Feb 2024 11:35:26 -0800 > From: "Paula" > To: > Subject: [Histonet] Marking Pen > Message-ID: <009e01da59fc$ce13b680$6a3b2380$@biopath.org> > Content-Type: text/plain;? ? charset="us-ascii" > > Hello, > > > > We have been using KP Marker Plus pens for cassettes.? We have 2 Leica > Processors and 1 VIP6 processor.? The cassettes that go into the VIP6 are > smeared and some are almost smeared off completely.? The processors have the > same solutions in them. > > > > If anyone can shed some light as to why there is a difference, and if anyone > can recommend a better marking pen for us to try, I would appreciate the > feedback. > > > > Thank you, > > Paula > > > > ------------------------------ > > Message: 2 > Date: Thu, 8 Feb 2024 11:30:03 +0000 > From: "Piche, Jessica" > To: "histonet at lists.utsouthwestern.edu" >? ? ,? ? Paula > Subject: Re: [Histonet] Marking Pen > Message-ID: >? ? >? ? > Content-Type: text/plain; charset="us-ascii" > > Hi Paula, > > What is the first solution the cassettes go in when the processor starts? We use Statmark pens when our cassette printer isn't working, and they work well. The only time we have had issues was when we would hand write the cassettes and run them on our small biopsy run which skips the formalin. It seems like the marker ink needs to "fix" with the formalin. Sometimes they smear if they aren't dry enough before they go into formalin too. I hope you figure it out. Maybe see if you can get some samples of different pens and then run some empty cassettes and see what works best for you. > > Have a good day. > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory > Histology Team Leader > 203-573-7167 > ________________________________ > From: Paula via Histonet > Sent: Wednesday, February 7, 2024 2:35 PM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Marking Pen > > [EXTERNAL MSG] > > Hello, > > > > We have been using KP Marker Plus pens for cassettes.? We have 2 Leica > Processors and 1 VIP6 processor.? The cassettes that go into the VIP6 are > smeared and some are almost smeared off completely.? The processors have the > same solutions in them. > > > > If anyone can shed some light as to why there is a difference, and if anyone > can recommend a better marking pen for us to try, I would appreciate the > feedback. > > > > Thank you, > > Paula > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C873b23e6f59c40f09ac508dc2813f9d3%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638429313478635896%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=eiD1an%2F2n73QFobrsXKdiC2Kk8h6AE78xAtW9DIRiAM%3D&reserved=0 > > > ------------------------------ > > Message: 3 > Date: Thu, 8 Feb 2024 17:19:28 +0000 > From: "Stephanie L. Thompson" > To: "Histonet at lists.utsouthwestern.edu" >? ? > Subject: [Histonet] Lead Histotechnologist > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Looking for the next step in your career? > > Sonic Healthcare USA has an opportunity for a Lead Histotechnologist. > > Quality is in our DNA -- is it in yours? > > Join our front line of #HealthcareHeroes! Our mission is to advance the health and wellbeing of our communities as a leader in clinical laboratory solutions. > > Location: Exeter, NH > Days: Monday - Friday > 9:00 PM - 5:30 AM > Full-time: Benefit Eligible > > In this role, you will: > > Prepare histologic slides from human tissue sections for microscopic examination and diagnosis by Pathologist. > >? *? Exercises independent judgment in dealing with procedural and technical problems. >? *? Examines slides and/or blocks to ensure tissue preparation is meeting laboratory requirements. >? *? Trains or directs Laboratory Assistants and Histology Technicians engaged in laboratory testing and processing techniques. >? *? Prepares sections of human tissue for microscopic examination and patient diagnosis, using techniques to gross (dissect tissue), embed (orient specimen in paraffin block), section (cut thin sections of tissue), stain (enhance contrast of tissue and highlight specific features of interest with routine hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass slides), from surgical procedures. >? *? Performs recuts and additional stains including special and immunohistochemistry stains, as requested by a Pathologist. >? *? Operates computerized laboratory equipment to fix, dehydrate, and infiltrate with wax, tissue specimens to be preserved for study by Pathologist. >? *? May label requisitions, specimen containers, cassettes and/or slides and affixes coverslip to slides. >? *? Maintains laboratory equipment and tracks all routine maintenance and quality controls performed. >? *? Files, retrieves, and distributes blocks, slides, and pathology reports. >? *? Operates, cleans, and sterilizes laboratory equipment, glassware, instruments, and workstation. >? *? Disposes of hazardous chemical waste per regulatory guidelines. >? *? Maintains strictest confidentiality. >? *? Complies with all State, Federal, professional regulations as well as company and departmental rules, polices, and procedural manuals. >? *? Adherence to CAP, CLIA, State Regulations, HIPAA, Safety and OSHA Regulations. > > All you need is: > >? *? High School diploma or equivalent required. Associates or Bachelors of Science degree and completion of histotechnology program required. Certification as a histotechnician (HT) or histotechnologist (HTL) by American Society of Clinical Pathology (ASCP) preferred. >? *? State licensure, if applicable. >? *? Certified or eligible for Board of Certification (BOC) by the American Society of Clinical Pathologists (ASCP) >? *? Completion of a Histology program accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year experience as a full-time Histology Technician Trainee and competent in the areas of fixation, processing, embedding, microtomy, grossing, special stains, immunohistochemistry, and lab operations. > > Company: > Sonic Anatomic Pathology > > Please feel free to contact me: > Stephanie Thompson - 210-428-1646 > > > > This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 243, Issue 6 > **************************************** _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmargaryan88 at gmail.com Thu Feb 8 15:25:47 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Thu, 8 Feb 2024 15:25:47 -0600 Subject: [Histonet] Modified Davidson Message-ID: Hello, Could you please share your best recipe for the Modified Davidson? Thanks in advance, Naira From afhenwood at outlook.com Thu Feb 8 16:31:52 2024 From: afhenwood at outlook.com (Tony Henwood) Date: Thu, 8 Feb 2024 22:31:52 +0000 Subject: [Histonet] Modified Davidson In-Reply-To: References: Message-ID: There are several formulations (some are probably typos), but this seems to be one commonly cited. Modified Davidson's Fixative: (Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicologic pathology, 30(4), 524-533.) 37?40% formaldehyde 30ml Absolute ethanol 15ml Glacial acetic acid 5ml Distilled Water 50ml Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Naira Margaryan via Histonet Sent: Friday, February 9, 2024 8:25:47 AM To: Histonet Subject: [Histonet] Modified Davidson Hello, Could you please share your best recipe for the Modified Davidson? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Thu Feb 8 23:46:51 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 9 Feb 2024 05:46:51 +0000 Subject: [Histonet] Modified Davidson In-Reply-To: References: Message-ID: The "Davidson's" name is, as Tony says, applied to various mixtures. In these, formalin is acidified with acetic acid in solutions that also contain some alcohol (typically about 33% v/v) but probably not enough to contribute to fixation. The mixture quoted from Latendresse et al. 2002 has only 15% alcohol - about the same as in a good dry sherry. (My gastric mucosa is not yet fixed.) The Histonet archives must surely still contain Dr Bob Richmond's contributions about "Davidson's fixative" in the 1990s or 2000s. Enough from me for now! John Kiernan London, Canada = = = ________________________________ From: Tony Henwood via Histonet Sent: February 8, 2024 5:31 PM To: Naira Margaryan ; Histonet Subject: Re: [Histonet] Modified Davidson There are several formulations (some are probably typos), but this seems to be one commonly cited. Modified Davidson's Fixative: (Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicologic pathology, 30(4), 524-533.) 37?40% formaldehyde 30ml Absolute ethanol 15ml Glacial acetic acid 5ml Distilled Water 50ml Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. ________________________________ From: Naira Margaryan via Histonet Sent: Friday, February 9, 2024 8:25:47 AM To: Histonet Subject: [Histonet] Modified Davidson Hello, Could you please share your best recipe for the Modified Davidson? Thanks in advance, Naira _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEB2AE at uvahealth.org Fri Feb 9 04:40:40 2024 From: JEB2AE at uvahealth.org (Brazie, Jeneanne E *HS) Date: Fri, 9 Feb 2024 10:40:40 +0000 Subject: [Histonet] tissue cassettes Message-ID: Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes to sit dry (no wax) while in the embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic?? From tpodawiltz at yahoo.com Fri Feb 9 08:33:41 2024 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Fri, 9 Feb 2024 14:33:41 +0000 (UTC) Subject: [Histonet] tissue cassettes In-Reply-To: References: Message-ID: <862745378.212546.1707489221220@mail.yahoo.com> Without seeing the blocks, that sounds more like cold molds being used, more Then, whether or not the tissues are kept in a dry, hot, well, or a wet well. Sent from Yahoo Mail for iPad On Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet wrote: Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes? to sit dry (no wax) while in the embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic?? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Fri Feb 9 09:52:57 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 9 Feb 2024 16:52:57 +0100 Subject: [Histonet] tissue cassettes In-Reply-To: References: Message-ID: <000601da5b70$0e3833c0$2aa89b40$@gmx.at> Hi, Since we have turned to embedding centers in the late 80ies we let the cassettes sit in the centers without additional paraffin. We only see such "jumping out" tissue, when the cassettes are not warmed (let the lid open) and the tissue renders too cold. As a result tissue and paraffin don't combine well enough. Maybe it is a matter of embedding technique? Too little paraffin in the mold before setting the tissue in? Cold embedding molds? Slow handling? Gudrun -----Urspr?ngliche Nachricht----- Von: Brazie, Jeneanne E *HS via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 9. Februar 2024 11:41 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] tissue cassettes Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes to sit dry (no wax) while in the embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic?? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VKurth at uwhealth.org Fri Feb 9 10:04:44 2024 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Fri, 9 Feb 2024 16:04:44 +0000 Subject: [Histonet] tissue cassettes In-Reply-To: <862745378.212546.1707489221220@mail.yahoo.com> References: <862745378.212546.1707489221220@mail.yahoo.com> Message-ID: I am old school and prefer them dry, lol. I agree with Thomas, that shouldn't have that affect. Ginny -----Original Message----- From: Thomas Podawiltz via Histonet Sent: Friday, February 9, 2024 8:34 AM To: Brazie, Jeneanne E *HS ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tissue cassettes WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Without seeing the blocks, that sounds more like cold molds being used, more Then, whether or not the tissues are kept in a dry, hot, well, or a wet well. Sent from Yahoo Mail for iPad On Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet wrote: Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes to sit dry (no wax) while in the embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic?? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Fri Feb 9 12:13:18 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 9 Feb 2024 13:13:18 -0500 Subject: [Histonet] RELIA HOT Job Alert - A Stellar Management Opportunity in an Unusual place. Message-ID: <01ce01da5b83$a8b52c60$fa1f8520$@earthlink.net> Hi Histonetters/Histopeeps! I have a Stellar Management Opportunity in South Dakota! Do you long for the outdoorsy life? How about Small-Town living? Do you love shows like Yellowstone so much you want to move out west? Are you ready for the next step in your career - Leadership? Then WE NEED TO TALK!! I am working with a client in Rapid City South Dakota in need of a histology manager. If you are: A Lead Tech A Histology Supervisor A Histology Manager You want to check this out! ASCP certified and willing to do some bench work and manage a small staff. This is a full time permanent position. My client is offering an excellent compensation package. For more information, please shoot me an email to relia1 at earthlink.net Thanks - Pam #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From rsrichmond at gmail.com Fri Feb 9 12:55:03 2024 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 9 Feb 2024 13:55:03 -0500 Subject: [Histonet] Histonet Digest, Vol 243, Issue 7 In-Reply-To: References: Message-ID: > > Re: [Histonet] Modified Davidson > > The formula for "modified Davidson's fixative" that I used was three parts tap water, three parts reagent alcohol, two parts 37% formaldehyde ("strong formalin"), one part glacial acetic acid. Best mixed under a fume hood. At 85 I must be one of the last left standing with much experience with it. I learned it when I was a resident at Johns Hopkins around 1970, when surgical pathologist Bill Hartmann introduced it at the request of medical geneticist Victor McCusick, who wanted it for fixing skin biopsy specimens to look for nuclear sex chromatin bodies ("Barr bodies") as specified by Moore & Barr in their 1950s publications on this important discovery. The fixative got quite popular with the GYN pathologists, who ran an entirely separate service, replacing their old "Vandegrift's fixative" for all their specimens. From there it seems to have spread to other pathology services, eventually becoming various proprietary fixatives such as "O-Fix". You could always identify it by the characteristic "airplane dope" (for boys)" or "nail polish remover" (for girls) aroma, as ethyl acetate accumulated in the aging fixative as the alcohol and acetic acid slowly esterified. The "modified" referred to the omission of glycerol from what was supposed to be Davidson's original formula. John Kiernan some years ago noted on Histonet that the formula wasn't entirely rational, and was very tolerant of various modifications. He said then that when he retired he'd try to find Davidson's original source among Davidson's unpublished papers. I think Davidson's fixative became largely obsolete for two reasons. Better embedding waxes gave much improved nuclear detail over plain old paraffin wax (what I remember long ago). And it isn't compatible with immunohistochemical techniques. Bob Richmond Maryville, Tennessee From carl.hobbs at kcl.ac.uk Fri Feb 9 13:12:16 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Fri, 9 Feb 2024 19:12:16 +0000 Subject: [Histonet] tissue cassettes Message-ID: I'm interested but don't understand the variation and it's + effect I take my cassettes out of the processor and immediately place into the molten wax bath of the embedder ( if I'm embedding immediately; if not I let the cassettes/tissues therein go cold until a later embedding) When embedding immediately, after 30 mins, I remove tissue from cassette into a heated metal mold filled with molten wax All done quickly, to keep all wax molten If my people take too long, the wax around the tissue sets so when the tissue is placed into the molten wax, in mold, they sometimes get that effect of set wax-interface artefact....the section immediately pulls apart from the surrounding wax, on the waterbath This can cause the section to fold Do enlighten me Thanks Carl Never too old to learn! Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From jmacdonald at mtsac.edu Fri Feb 9 18:40:18 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Sat, 10 Feb 2024 00:40:18 +0000 Subject: [Histonet] tissue cassettes In-Reply-To: References: Message-ID: We embed from cold and this happens if the tissue is not brought up to the temperature and the wax is melted. -----Original Message----- From: Carl Hobbs via Histonet Sent: Friday, February 9, 2024 11:12 AM To: histonet Subject: Re: [Histonet] tissue cassettes EXTERNAL SENDER - Exercise caution with requests, links, and attachments. I'm interested but don't understand the variation and it's + effect I take my cassettes out of the processor and immediately place into the molten wax bath of the embedder ( if I'm embedding immediately; if not I let the cassettes/tissues therein go cold until a later embedding) When embedding immediately, after 30 mins, I remove tissue from cassette into a heated metal mold filled with molten wax All done quickly, to keep all wax molten If my people take too long, the wax around the tissue sets so when the tissue is placed into the molten wax, in mold, they sometimes get that effect of set wax-interface artefact....the section immediately pulls apart from the surrounding wax, on the waterbath This can cause the section to fold Do enlighten me Thanks Carl Never too old to learn! Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Sat Feb 10 10:31:32 2024 From: patpxs at gmail.com (patpxs) Date: Sat, 10 Feb 2024 08:31:32 -0800 Subject: [Histonet] tissue cassettes In-Reply-To: Message-ID: <65c7a4e5.170a0220.46f26.9ccf@mx.google.com> Hi Carl,The bon-bon effect.? This can happen if the wax used for processing is different from the wax used for infiltration or when the tissue gets cold before embedding.Either way, because the tissue is cold - the embedding wax doesn't blend with the tissue wax, creating the hard outer wax shell.??The simplest way to avoid the bon-bon effect is to make sure the tissue is as warm as the wax it's being embedded in.??Letting the cassettes go cold isn't the issue here, it's not letting the tissue warm up before embedding that is.? I call it "freezing" the tissue/cassette, since that's what the wax is doing, just to mess with people's heads.? That helps remind people that the wax gets cold quickly.Some folks may disagree with me about that.If the embedding wax is a different from the infiltration wax it's kind of the same thing.? The waxes need to co-mingle a little while so they can blend together before freezing the block.Just a few ideas...PaulaSent from Samsung tablet -------- Original message --------From: Carl Hobbs via Histonet Date: 2/9/24 11:12 AM (GMT-08:00) To: histonet Subject: Re: [Histonet] tissue cassettes I'm interested but don't understand the variation and it's + effectI take my cassettes out of the processor and immediately place into the molten wax bath of the embedder ( if I'm embedding immediately; if not I let the cassettes/tissues therein go cold until a later embedding)When embedding immediately, after 30 mins, I remove tissue from cassette into a heated metal mold filled with molten waxAll done quickly, to keep all wax moltenIf my people take too long, the wax around the tissue sets so when the tissue is placed into the molten wax, in mold, they sometimes get that effect of set wax-interface artefact....the section immediately pulls apart from the surrounding wax, on the waterbathThis can cause the section to foldDo enlighten meThanksCarlNever too old to learn!Carl Hobbs FIBMSHistology and Imaging ManagerWolfson SPaRCGuys Campus, London BridgeKings College LondonLondonSE1 1UL020 7848 6810_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Sat Feb 10 10:42:20 2024 From: patpxs at gmail.com (patpxs) Date: Sat, 10 Feb 2024 08:42:20 -0800 Subject: [Histonet] tissue cassettes In-Reply-To: Message-ID: <65c7a76d.630a0220.5f691.c796@mx.google.com> I'm old, old school too.? I prefer the paraffin pool myself, it helps prevent the bon-bon effect.? I had a vendor rep tell me the holding bins were designed for molten paraffin.Probably what happened over the years is that if there's too much molten wax in the bin, it over flows when the basket of cassettes is put in.Ah, displacement, just like when you get into an over full bathtub, water (or wax) everywhere.?Now a days I think the dry holding tank is the rage.? As long as the tissue stays warm, it probably doesn't matter too much.?Ciao bellas,PaulaSent from Samsung tablet -------- Original message --------From: "Kurth, Virginia L via Histonet" Date: 2/9/24 8:04 AM (GMT-08:00) To: Thomas Podawiltz , "Brazie, Jeneanne E *HS" , histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] tissue cassettes I am old school and prefer them dry, lol.? I agree with Thomas, that shouldn't have that affect.Ginny-----Original Message-----From: Thomas Podawiltz via Histonet Sent: Friday, February 9, 2024 8:34 AMTo: Brazie, Jeneanne E *HS ; histonet at lists.utsouthwestern.eduSubject: Re: [Histonet] tissue cassettesWARNING: This email appears to have originated outside of the UW Health email system.DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.Without seeing the blocks, that sounds more like cold molds being used, more Then, whether or not the tissues are kept in a dry, hot, well, or a wet well.Sent from Yahoo Mail for iPadOn Friday, February 9, 2024, 6:00 AM, Brazie, Jeneanne E *HS via Histonet wrote:Hello :) I am encountering push back in our lab when I fill the embedding units with melted paraffin in the embedding wells. The techs here like for the tissue cassettes? to sit dry (no wax) while in the? embedding units. I find that the tissue rolls out of the sections while cutting because of a layering effect between the tissue and the paraffin its embedded in. I have communicated this but they tell me I'm "old school". Does anyone have any thoughts or opinions on this topic??_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Sat Feb 10 13:22:48 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Sat, 10 Feb 2024 19:22:48 +0000 Subject: [Histonet] tissue cassettes Message-ID: Why would anyone use a different wax for infiltrating and embedding? Yes....keep them specimens molten until embedded Thanks for your input, Paula Time flies like an arrow Fruit flies like.... a banana BonBon-illy Carl From patpxs at gmail.com Sat Feb 10 21:18:46 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Sun, 11 Feb 2024 03:18:46 +0000 (UTC) Subject: [Histonet] tissue cassettes In-Reply-To: References: Message-ID: <466720764.757327.1707621526672@mail.yahoo.com> Why use a different wax for embedding?? Cost. Those who don't fully understand the art & science of histology think a wax is a wax is a wax. Bon-bon, Paula Sent from Yahoo Mail on Android On Sat, Feb 10, 2024 at 11:23 AM, Carl Hobbs via Histonet wrote: Why would anyone use a different wax for infiltrating and embedding? Yes....keep them specimens molten until embedded Thanks for your input, Paula Time flies? like an arrow Fruit? flies like.... a banana BonBon-illy Carl _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carlos.torresvega at gmail.com Mon Feb 12 11:39:55 2024 From: carlos.torresvega at gmail.com (Carlos Torres Vega) Date: Mon, 12 Feb 2024 11:39:55 -0600 Subject: [Histonet] Asking for CV-5000 cover slipper sewrvice software. Message-ID: Does anybody have the software for service a Leica CV-5000 cover slippers. I will appreciate a lot if any body can share it to me. Thanks a lot From relia1 at earthlink.net Mon Feb 12 11:45:19 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 12 Feb 2024 12:45:19 -0500 Subject: [Histonet] ASCP certified and experienced tech relocating to IL. Can you Help? Message-ID: <000001da5ddb$4125d3b0$c3717b10$@earthlink.net> Hello Histonetters/Histopeeps! I hope this is the start to a great week for you. I am dropping you a quick line because I am working with an amazing histotech that is relocating to IL. My candidate is ASCP certified and experienced in both routine and Mohs histology. Please let me know if you or anyone you know could use a tech like this. If you are curious how my services work, I can send you info. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From carlos.torresvega at gmail.com Mon Feb 12 12:11:54 2024 From: carlos.torresvega at gmail.com (Carlos Torres Vega) Date: Mon, 12 Feb 2024 12:11:54 -0600 Subject: [Histonet] Fwd: Asking for CV-5000 cover slipper sewrvice software. References: Message-ID: Enviado desde mi iPhone Inicio del mensaje reenviado: > De: Carlos Torres Vega > Fecha: 12 de febrero de 2024, 11:39:55?a.m. GMT-6 > Para: histonet at lists.utsouthwestern.edu > Asunto: Asking for CV-5000 cover slipper sewrvice software. > > ? > Does anybody have the software for service a Leica CV-5000 cover slippers. > I will appreciate a lot if any body can share it to me. > Thanks a lot From luzwig at gmail.com Mon Feb 12 17:49:18 2024 From: luzwig at gmail.com (Luz Linares) Date: Mon, 12 Feb 2024 15:49:18 -0800 Subject: [Histonet] decalcification Message-ID: Does anybody have a good protocol for decalcified teeth? I will really appreciated Luz From gu.lang at gmx.at Tue Feb 13 08:41:50 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 13 Feb 2024 15:41:50 +0100 Subject: [Histonet] microwave processing Message-ID: <000d01da5e8a$c882e410$5988ac30$@gmx.at> Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstra?e 25 4040 Linz From ewj at pigs.ag Wed Feb 14 21:36:52 2024 From: ewj at pigs.ag (ewj at pigs.ag) Date: Thu, 15 Feb 2024 11:36:52 +0800 Subject: [Histonet] microwave processing In-Reply-To: <000d01da5e8a$c882e410$5988ac30$@gmx.at> References: <000d01da5e8a$c882e410$5988ac30$@gmx.at> Message-ID: <15507b3854cc005818cfd582bfd29663af30b6d8.camel@pigs.ag> We have a sakura VIP5 but we have been processing most of our histopath with DIY-modified microwave and no xylene for several years now. ?We still use the old VIP5 in xylene mode for large batches of stuff just to save labor time. I have no idea what others do. ? E. Wayne Johnson DVM Enable AgTech Beijing -----Original Message----- From: Gudrun Lang via Histonet Reply-To: Gudrun Lang To: histonet at lists.utsouthwestern.edu Subject: [Histonet] microwave processing Date: Tue 10:41 PM Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstra?e 25 4040 Linz _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcokertx at gmail.com Thu Feb 15 11:41:33 2024 From: mcokertx at gmail.com (Michelle Bell) Date: Thu, 15 Feb 2024 17:41:33 +0000 Subject: [Histonet] microwave processing In-Reply-To: <15507b3854cc005818cfd582bfd29663af30b6d8.camel@pigs.ag> References: <000d01da5e8a$c882e410$5988ac30$@gmx.at> <15507b3854cc005818cfd582bfd29663af30b6d8.camel@pigs.ag> Message-ID: From the vendor perspective, we have definitely seen an increase in implementation of our processors. Going to the xylene-free, isopropanol clearing approach has lead to converts because the process is more gentle (xylene can harden dense tissues and over clear small specimens). Before coming to work on the vendor side, I have been using this technology for over 20 years :) ________________________________ From: ewj--- via Histonet Sent: Wednesday, February 14, 2024 9:36:52 PM To: Gudrun Lang ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] microwave processing We have a sakura VIP5 but we have been processing most of our histopath with DIY-modified microwave and no xylene for several years now. We still use the old VIP5 in xylene mode for large batches of stuff just to save labor time. I have no idea what others do. E. Wayne Johnson DVM Enable AgTech Beijing -----Original Message----- From: Gudrun Lang via Histonet Reply-To: Gudrun Lang To: histonet at lists.utsouthwestern.edu Subject: [Histonet] microwave processing Date: Tue 10:41 PM Dear histonetters! I have a question for those who have an insight in the whole landscape of pathologies. I am reading the microwave application book of Dr. Leong (2009). He writes very enthusiastic about fixation and processing with mircrowaves. I know there are microwave processors for continous workflow on the market. Now I am curious, how many pathologies use this technology. What do you think? A few percent or rising numbers? Thanks in advance and kind regards Gudrun Gudrun Lang Landgutstra?e 25 4040 Linz _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdean70 at hotmail.com Sat Feb 24 15:15:39 2024 From: kdean70 at hotmail.com (Ken M) Date: Sat, 24 Feb 2024 21:15:39 +0000 Subject: [Histonet] Control Slides Message-ID: May I ask, in all your opinions, Who is supplying the best control slides for clinical settings? Both Special Stain and IHC? What would you like to see in your control slides? What don't you want to see- long lead times, quality? As for Research, what are your requirements? From rsrichmond at gmail.com Sun Feb 25 18:10:59 2024 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 25 Feb 2024 19:10:59 -0500 Subject: [Histonet] Control Slides (Ken M) Message-ID: Here's the opinion of an 85 year old pathologist who in the course of his training spent a year doing histochemical research. In my experience, pathologists aren't allowed much input in the selection of controls. Anyone doing special stains needs to be able to evaluate the control slides for adequacy. Control tissue needs to be fixed and processed in the same way as the tissue being investigated. Some stains (like trichromes) need controls only to keep the inspectors happy, while others need a control run and looked at every time. Stains for micro-organisms require controls, for example. These controls should be tissue sections with active infection, not bugs embedded in gelatin. These controls are hard to get. Acid-fast stains require a control for the particular organism being looked for. Usually Mycobacterium tuberculosis. The best controls I've ever seen were lungs from rhesus monkeys with active tuberculosis, which a lot of them imported for research purposes had. The veterinary pathologists used to do these necropsies. Obviously one monkey can keep you supplied for a lifetime. Myco. leprae is a notoriously fickle stainer, and controls are hard to get, since very few animals can be infected with human leprosy. One leprous armadillo liver can supply a laboratory for many years. I think atypical mycobacteria usually get tuberculosis controls. The chromic acid methenamine silver stain is best controlled with the particular fungus you're looking for. The most demanding control is histoplasma in old inactive lung lesions. Candida is probably too easy. PAS really isn't a satisfactory fungus stain. Tissue Gram stains are vastly over-rated, and should be done away with. I think most people use a diseased appendix. If you're still doing a Giemsa or toluidine blue stain for Helicobacter, it should be controlled with a known positive for that organism. Amyloid controls have to be repeatedly tested to make sure they work. Human amyloidosis is a rare disease, and autopsy material these days is much rarer. I wonder if amyloid from animal controls has ever come into use. (Amyloidosis is supposed to be rather easily produced in mice.) I've never been able to get an answer to this question. Supposedly amyloid in sections still in wax on the slide (as controls are commonly prepared) isn't stable for more than a month. Bob Richmond Maryville TN I don't have enough technical experience with immunohistochemistry to have any ideas. From relia1 at actsend.com Mon Feb 26 12:04:57 2024 From: relia1 at actsend.com (Pam Barker) Date: Mon, 26 Feb 2024 18:04:57 +0000 Subject: [Histonet] Can you believe it's been 19 years? Check it out!!! Message-ID: <19.34.23217.9C2DCD56@gx.mta1vrest.cc.prd.sparkpost> Hi Histopeeps, How are you doing today?? This is the start to an exciting week for me! This week we are celebrating RELIA?s 19th year in business. Thank You!! For the past 19 years we have worked exclusively in the nationwide permanent placement of histology professionals.? I have felt so privileged to have helped so many people with their careers and look forward to helping many more! We strive to be the best at making those perfect matches between labs and histopeeps! I know your inbox is precious real estate and I appreciate you taking the time to read my emails. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA?Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:???????(407)353-5070 FAX:???????(407)678-2788 E-mail:?relia1 at earthlink.net??? https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histology #histologylab #histologyjobs #hmuhistopeeps Click Here to Unsubscribe? RELIA Solutions? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ? ?? histonet at lists.utsouthwestern.edu From modz9636 at gmail.com Tue Feb 27 11:50:55 2024 From: modz9636 at gmail.com (M.O.) Date: Tue, 27 Feb 2024 09:50:55 -0800 Subject: [Histonet] Leak under Sakura Tissue-Tek TEC 5 embedding machine Message-ID: Hello, Has anyone encountered a leak of liquid paraffin under their embedding machine? We have a TEC 5 and I'm not sure where to start in trying to locate the leak. If anyone has any insights, that would be great! Thank you, Merissa From travis at imebinc.com Tue Feb 27 12:31:56 2024 From: travis at imebinc.com (travis at imebinc.com) Date: Tue, 27 Feb 2024 10:31:56 -0800 Subject: [Histonet] Leak under Sakura Tissue-Tek TEC 5 embedding machine In-Reply-To: References: Message-ID: <008101da69ab$3e5171e0$baf455a0$@imebinc.com> Hello, It's a pretty common issue that shouldn't impact your embedding. The TEC 5 is not air tight, so when its moved with paraffin filled to the rim, some of it spills and falls into the cracks. Over time it builds up inside the unit by melting and getting hard again. Until one day it moves all the way through unit and leaks out at the lowest point (normally under one of the two chambers). When we refurbished these units we open them up to find them filled with paraffin but, it doesn?t really cause any problems other than a mess under the unit. ? Travis O?Brien International Medical Equipment, Inc. 170 Vallecitos De Oro San Marcos, CA 92069 800.543.8496 Phone 760.761.0859 Fax www.imebinc.com -----Original Message----- From: M.O. via Histonet Sent: Tuesday, February 27, 2024 9:51 AM To: Histonet Subject: [Histonet] Leak under Sakura Tissue-Tek TEC 5 embedding machine Hello, Has anyone encountered a leak of liquid paraffin under their embedding machine? We have a TEC 5 and I'm not sure where to start in trying to locate the leak. If anyone has any insights, that would be great! Thank you, Merissa _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Tue Feb 27 13:46:25 2024 From: plucas at biopath.org (Paula) Date: Tue, 27 Feb 2024 11:46:25 -0800 Subject: [Histonet] preordering giemsa stains Message-ID: <000001da69b5$a735d110$f5a17330$@biopath.org> Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager From Jessica.Piche at wtbyhosp.org Wed Feb 28 05:06:02 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Wed, 28 Feb 2024 11:06:02 +0000 Subject: [Histonet] preordering giemsa stains In-Reply-To: <000001da69b5$a735d110$f5a17330$@biopath.org> References: <000001da69b5$a735d110$f5a17330$@biopath.org> Message-ID: Hi Paula, We do H.pylori on all specimens related to stomach upfront. We also do Ab Pas too. I think it is fairly common in lots of labs. Have a good day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: Paula via Histonet Sent: Tuesday, February 27, 2024 2:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] preordering giemsa stains [EXTERNAL MSG] Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C8c79a99470e944836df908dc37ccd260%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638446600040215608%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=KMVmHS4sxVL51GsP2QAidVLRrPWcKk4eJiZyE41ZFsA%3D&reserved=0 From relia1 at earthlink.net Wed Feb 28 11:13:59 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 28 Feb 2024 12:13:59 -0500 Subject: [Histonet] A Special Announcement! Introducing RELIA's NEW Reciprocal Referral Reward Program. New ways to Earn MORE! A RELIA EXCLUSIVE!! Message-ID: <012f01da6a69$8568d000$903a7000$@earthlink.net> Hi Histonetters!! I hope you are having a great day! Did you know some of my very best hires are with labs where I know histopeeps? Not Surprising! You guys are the BEST! So I figured let?s change things up a bit and see if we can get some more of those good vibes flowing! Introducing the RELIA Reciprocal Referral Program! A Reward Program EXCLUSIVE to RELIA! ? Our original referral program is still in place: If you refer someone to me that I place you will earn a referral bonus. THE RELIA RECIPROCAL REFERRAL Program works like this: If you have an open histology position in your lab and connect me with the right people resulting in one of my histopeeps coming to work you will earn a referral bonus. All you have to do is send me an email and let me know you have an opening along with the email address of your manager. This can be a confidential referral OR I can give you full credit to your manager for stepping up to help out with staffing! Whichever you prefer. OR In the emails that you receive from me you will see a list of locations where some of my best histopeeps want to be. If you have an opening in your lab in that area and connect me with the right people resulting in a histopeep on the list coming to work in your lab you will earn a referral bonus. For example: I post in my email: Histotech ? Atlanta and you are working in and have an opening in your lab in Atlanta if you put me in touch with your hiring manager and my histopeep gets hired you earn a referral bonus. All you have to do is send me an email and let me know you have an opening along with the email address of your manager. This can be a confidential referral OR I can give you the full credit to your manager for stepping up to help out with staffing! Whichever you prefer. The reciprocity is that a fellow histopeep gets a position in a great lab (because well YOU work there) and you will earn a referral bonus. I also have some great permanent positions if you or someone you know is interested in a new opportunity. Here are the locations for POSITIONS: ? South Dakota ? Florida ? California ? Virginia ? Alabama ? Georgia Here are the locations that I have HISTOPEEPS Looking: ? Ilinois ? Indiana ? Iowa ? California ? Florida ? Arizona ? Pennsylvania ? New Jersey/New York ? Texas ? Virginia ? Oregon ? North Carolina ? South Carolina For more information on my current openings or the candidates I am working with please contact me. You can reach me at mailto:relia1 at earthlink.net OR cell/text 407-353-5070 Have a Great Day! Thanks ? Pam! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From Diana.Martinez-Longoria at ecrmc.org Wed Feb 28 15:46:45 2024 From: Diana.Martinez-Longoria at ecrmc.org (Diana Martinez-Longoria) Date: Wed, 28 Feb 2024 21:46:45 +0000 Subject: [Histonet] Question Regarding HM355S Automatic Microtome Message-ID: <4738f68e9b42425eb217752962d3d42d@ecrmc.org> Hello All, I am reaching out to seek help regarding if anyone is currently or have in the past used the HM355S Automatic Microtome? What are the pros and cons? Thank you in Advance! Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. From Jessica.Piche at wtbyhosp.org Thu Feb 29 05:49:31 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 29 Feb 2024 11:49:31 +0000 Subject: [Histonet] Question Regarding HM355S Automatic Microtome In-Reply-To: <4738f68e9b42425eb217752962d3d42d@ecrmc.org> References: <4738f68e9b42425eb217752962d3d42d@ecrmc.org> Message-ID: Hi Diana, We are not currently using the HM355S in our lab, but we did demo it and were not fans. I would see if you can demo one to see if you like it. We had the Thermo Shandon Finesse and Finesse Me models and we loved them but the HM355S didn't appear to be designed the same way and we ended up getting the Leica Autocuts and love them!! Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: Diana Martinez-Longoria via Histonet Sent: Wednesday, February 28, 2024 4:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question Regarding HM355S Automatic Microtome [EXTERNAL MSG] Hello All, I am reaching out to seek help regarding if anyone is currently or have in the past used the HM355S Automatic Microtome? What are the pros and cons? Thank you in Advance! Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjessica.piche%40wtbyhosp.org%7C0013885952c2481ecedd08dc38a6cc32%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638447536260334923%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=bMagf%2BdmymCmdU%2BuwO4jgHD2CwhHNbUR3xHoEQjKVcY%3D&reserved=0 From Robyn.L.Vazquez at kp.org Thu Feb 29 08:38:01 2024 From: Robyn.L.Vazquez at kp.org (Robyn L Vazquez) Date: Thu, 29 Feb 2024 14:38:01 +0000 Subject: [Histonet] Question Regarding HM355S Automatic Microtome In-Reply-To: References: <4738f68e9b42425eb217752962d3d42d@ecrmc.org> Message-ID: Hello, I have been using this microtome for the past 12 years. Love it! Regular maintenances and it will last a longtime. Just changed the motherboard (expensive) and back in business. Can not thing of any cons all good. Robyn -----Original Message----- From: Piche, Jessica via Histonet Sent: Thursday, February 29, 2024 3:50 AM To: histonet at lists.utsouthwestern.edu; Diana Martinez-Longoria Subject: Re: [Histonet] Question Regarding HM355S Automatic Microtome Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ Hi Diana, We are not currently using the HM355S in our lab, but we did demo it and were not fans. I would see if you can demo one to see if you like it. We had the Thermo Shandon Finesse and Finesse Me models and we loved them but the HM355S didn't appear to be designed the same way and we ended up getting the Leica Autocuts and love them!! Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: Diana Martinez-Longoria via Histonet Sent: Wednesday, February 28, 2024 4:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question Regarding HM355S Automatic Microtome [EXTERNAL MSG] Hello All, I am reaching out to seek help regarding if anyone is currently or have in the past used the HM355S Automatic Microtome? What are the pros and cons? Thank you in Advance! Diana Martinez-Longoria El Centro Regional Medical Center Lead Histotechnician (ASCP)cm Laboratory - Pathology Department 1415 Ross Ave | El Centro, CA 92243 760.339.7267: Fax: 760-3394570 Diana.Martinez-Longoria at ecrmc.org ECRMC Confidentiality Notice: This e-mail is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, PLEASE contact the sender and promptly destroy this e-mail and its attachments. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__https://nam12.safelinks.protection.outlook.com/?url=http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet&data=05*7C02*7Cjessica.piche*40wtbyhosp.org*7C0013885952c2481ecedd08dc38a6cc32*7Cd4e8b650c4e4424481b4a11e2edb35a9*7C0*7C0*7C638447536260334923*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C0*7C*7C*7C&sdata=bMagf*2BdmymCmdU*2BuwO4jgHD2CwhHNbUR3xHoEQjKVcY*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUl!!BZ50a36bapWJ!uLGojk6U__2fXmVuxVEieYhquFGGVX64j5hf3NXofERXFi8V6AwopH6Khs5dlmLE9Df1OTbuANEzRW6jLBzgCoaVwPkGSFnMYx08$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!uLGojk6U__2fXmVuxVEieYhquFGGVX64j5hf3NXofERXFi8V6AwopH6Khs5dlmLE9Df1OTbuANEzRW6jLBzgCoaVwPkGSCI40waQ$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. From gu.lang at gmx.at Thu Feb 29 08:48:04 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 29 Feb 2024 15:48:04 +0100 Subject: [Histonet] preordering giemsa stains In-Reply-To: <000001da69b5$a735d110$f5a17330$@biopath.org> References: <000001da69b5$a735d110$f5a17330$@biopath.org> Message-ID: <002401da6b1e$4d908a00$e8b19e00$@gmx.at> In my place it is ordered on demand, but the pathologists usually perfer IHC for Hp. Gudrun -----Urspr?ngliche Nachricht----- Von: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 27. Februar 2024 20:46 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] preordering giemsa stains Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick at northwestern.edu Thu Feb 29 14:14:56 2024 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Thu, 29 Feb 2024 20:14:56 +0000 Subject: [Histonet] preordering giemsa stains In-Reply-To: <002401da6b1e$4d908a00$e8b19e00$@gmx.at> References: <000001da69b5$a735d110$f5a17330$@biopath.org> <002401da6b1e$4d908a00$e8b19e00$@gmx.at> Message-ID: We used to Alcian Yellow... Bernice -----Original Message----- From: Gudrun Lang via Histonet Sent: Thursday, February 29, 2024 8:48 AM To: 'Paula' Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] preordering giemsa stains In my place it is ordered on demand, but the pathologists usually perfer IHC for Hp. Gudrun -----Urspr?ngliche Nachricht----- Von: Paula via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 27. Februar 2024 20:46 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] preordering giemsa stains Hello, Is it a common practice to preorder stains that involve the stomach searching for H pylori? Or, is it more common to order as needed? Thank you for anyone's feedback. Paula Lucas Lab Manager _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!X7dgilKf4-E3EhKyDtv4fhEtpCU8Wn2T2DDP5BXwZz1QBzrRzg4fSvYjkrZlIXOzt4OeVj_obLymNiZvAoblSsCoe5C7VBQN6GFIavSx$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!X7dgilKf4-E3EhKyDtv4fhEtpCU8Wn2T2DDP5BXwZz1QBzrRzg4fSvYjkrZlIXOzt4OeVj_obLymNiZvAoblSsCoe5C7VBQN6GFIavSx$ From djemge11 at gmail.com Thu Feb 29 16:15:40 2024 From: djemge11 at gmail.com (Donna Emge) Date: Thu, 29 Feb 2024 16:15:40 -0600 Subject: [Histonet] Question Regarding HM355S Automatic Microtome Message-ID: Diana, I have used the HM355S at two different labs and liked it. Make sure to demo it or purchase it with the ?E? type disposable blade carrier. No one liked the ?ER? type blade carrier. You can see the difference between them in the operator manual -?just do a browser search for the manual. I also really liked using the foot pedal to stop and start. The memory feature makes fast work of. trimming blocks. When you put the next block in the clamp, push the memory button and it retracts to the exact position needed to start trimming the block. Donna Emge