From relia1 at earthlink.net Thu Aug 1 11:25:55 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 1 Aug 2024 12:25:55 -0400 Subject: [Histonet] Happy Thursday Histonetters!! Message-ID: <019f01dae42f$8423b760$8c6b2620$@earthlink.net> Happy Thursday, Histopeeps! ? Can you believe it?s already August 1st? ? ICYMI - Throwback Thursday: If you missed out on the RELIA Histopeep Summer Splash 2024, there?s still time to join us! Dive into: Histology Art ? Resume Tune-Ups ? Histology Haiku ?? Blog Posts ? Career Advice ? Fantastic Job Opportunities ? Reach out to me: Message me on social media ? Email: relia1 at earthlink.net ? Call/Text: 407-353-5070 ? Check out the hashtag: #RELIASummerSplash2024 ? Catch up and have a fantastic day! ? Thanks, Pam #Thursday #ThrowbackThursday #RELIASummerSplash2024 #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers From rfisher0126 at msn.com Thu Aug 1 13:39:01 2024 From: rfisher0126 at msn.com (Renee Fisher) Date: Thu, 1 Aug 2024 18:39:01 +0000 Subject: [Histonet] IHC help Message-ID: Does anyone have a technique that will keep mice skin tissue on the slide through the retrieval process. We are using positive charged slides, but still have tissue lifting. We do digital imaging and AI the lifting of the tissue affects the AI. Get Outlook for iOS From edmartin26 at gmail.com Thu Aug 1 16:12:50 2024 From: edmartin26 at gmail.com (Eddie Martin) Date: Thu, 1 Aug 2024 17:12:50 -0400 Subject: [Histonet] Response to Considering whether starting your own repository due to high quantity of tissues already in your posession that are being discarded and alternately used for profitable gain In-Reply-To: References: Message-ID: Hello Curt, Regarding your question: So running a ref lab for years I've acquired a rather large supply of old blocks of various tissues, I'm considering turning this into a tissue bank/repository with the purpose of selling blocks for research and control purposes... anyone have any experience, what are the requirements to be compliant??? I have zero experience with this. I'm guessing some release forms, maybe patient consent forms (IMPOSSIBLE TO GET THOSE FOR BLOCKS THAT ARE 10 YRS OLD)... release of liability forms... I'm starting at zero so any insight is appreciated! *Note 1: I'm neither a pathologist, a lawyer or any kind of adequate legal counsel. The response below is my opinion only, and my own understanding of the HIPAA act, and does not reflect the views of the institutions I work at or the employers I work for: I kindly suggest you seek permission from your company's medical-legal office, as they and not yourself individually are covered entities. Any instance of accessing a patient's medical diagnostic report for non-testing purposes that is also not assigned to you by a clinician/pathologist of accessing medically necessary information to perform tasks covered and protected by the covered entity, including testing necessary to perform for the patient's medical care and continuation of care is a breach of a patient's PHI information, whether accessed via hardcopy or electronic or any other kind of format. I can easily think of four Federal Acts in place protecting PHI: ** HIPAA, HITECH, FERPA, GINA *** Then individual State Laws that each may have even more strict guidelines in place to protect PHI. I get that you are trying to repurpose pathology materials, but established repositories are regulated by the FDA in addition to other federal and State agencies. Seeking the permission of your company's medical-legal department is highly suggested before accessing anything. My understanding of the intricacies of what I'm posting is limited in scope and understanding. From my understanding of the HIPAA Act above, each instance of breach must be reported to your company within sixty days of becoming aware of the breach, unless your local and State authorities have more stringent laws in place; and may subject your employer and yourself to breaching the confidentiality, integrity and availability made available in accessing the PHI, that also outlines standards and penalties for non-compliance. In the event the quantity of breaches exceeds 500 individuals impacted, the covered entity must also report the breach to the Department of Health and Human Services and provide the information to prominent media outlets to inform possible patients impacted by the breach within the jurisdiction the breach had occurred. Very respectfully, Eddie Martin, HT, HTL, QIHC On Thu, Aug 1, 2024 at 1:00?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Tissue repository (Curt Tague) > 2. Re: Tissue repository (Jay Lundgren) > 3. Re: Tissue repository (richard cartun) > 4. Re: Tissue repository (Curt Tague) > 5. Happy Thursday Histonetters!! (relia1 at earthlink.net) > > > > ---------- Forwarded message ---------- > From: Curt Tague > To: "Histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Wed, 31 Jul 2024 21:02:20 +0000 > Subject: [Histonet] Tissue repository > Hi all, > > So running a ref lab for years I've acquired a rather large supply of old > blocks of various tissues, I'm considering turning this into a tissue > bank/repository with the purpose of selling blocks for research and control > purposes... anyone have any experience, what are the requirements to be > compliant??? I have zero experience with this. I'm guessing some release > forms, maybe patient consent forms (IMPOSSIBLE TO GET THOSE FOR BLOCKS THAT > ARE 10 YRS OLD)... release of liability forms... I'm starting at zero so > any insight is appreciated! > > Best! > > Curt > > > > > > ---------- Forwarded message ---------- > From: Jay Lundgren > To: Curt Tague > Cc: "Histonet at lists.utsouthwestern.edu" > > Bcc: > Date: Wed, 31 Jul 2024 17:26:44 -0500 > Subject: Re: [Histonet] Tissue repository > Sorry Igor, but if you can't get informed consent, it's not legal or > ethical to sell patients' surgical specimens. There are laws in all 50 > states. The body or parts thereof have to be explicitly donated. > > If you have informed consent, you can sell them for a large profit, as lots > of non-transplant tissue banks do. That's a multi-million dollar, highly > profitable industry. How could it not be, getting something for free, and > selling it for top dollar. > > You might be thinking, "who's going to know?" In my experience, lots of > people are nosy and vindictive for no good reason. You could end up in > prison. Consult an attorney. > > Sorry to ruin your retirement plans. > > Sincerely, > > Jay A. Lundgren, M.S., HTL (ASCP) > > On Wed, Jul 31, 2024 at 4:02?PM Curt Tague via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi all, > > > > So running a ref lab for years I've acquired a rather large supply of old > > blocks of various tissues, I'm considering turning this into a tissue > > bank/repository with the purpose of selling blocks for research and > control > > purposes... anyone have any experience, what are the requirements to be > > compliant??? I have zero experience with this. I'm guessing some release > > forms, maybe patient consent forms (IMPOSSIBLE TO GET THOSE FOR BLOCKS > THAT > > ARE 10 YRS OLD)... release of liability forms... I'm starting at zero so > > any insight is appreciated! > > > > Best! > > > > Curt > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ---------- Forwarded message ---------- > From: richard cartun > To: "Histonet at lists.utsouthwestern.edu" , > Curt Tague > Cc: > Bcc: > Date: Thu, 1 Aug 2024 02:00:26 +0000 (UTC) > Subject: Re: [Histonet] Tissue repository > Hi Curt, > Does your lab have an in-house legal department? If so, have you > consulted with them about this? The attorneys for the hospital I worked in > wanted nothing to do with providing patient tissue to outside interests who > were willing to pay for it. > > Richard Cartun > > On Wednesday, July 31, 2024 at 05:02:57 PM EDT, Curt Tague via > Histonet wrote: > > Hi all, > > So running a ref lab for years I've acquired a rather large supply of old > blocks of various tissues, I'm considering turning this into a tissue > bank/repository with the purpose of selling blocks for research and control > purposes... anyone have any experience, what are the requirements to be > compliant??? I have zero experience with this. I'm guessing some release > forms, maybe patient consent forms (IMPOSSIBLE TO GET THOSE FOR BLOCKS THAT > ARE 10 YRS OLD)... release of liability forms... I'm starting at zero so > any insight is appreciated! > > Best! > > Curt > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ---------- Forwarded message ---------- > From: Curt Tague > To: richard cartun , " > Histonet at lists.utsouthwestern.edu" > Cc: > Bcc: > Date: Thu, 1 Aug 2024 02:28:58 +0000 > Subject: Re: [Histonet] Tissue repository > Thanks Richard, that?s a concern too, hospitals are certainly going to > avoid any exposure. > > Thanks again! > Curt > > Get Outlook for iOS > ________________________________ > From: richard cartun > Sent: Wednesday, July 31, 2024 7:00:26 PM > To: Histonet at lists.utsouthwestern.edu ; > Curt Tague > Subject: Re: [Histonet] Tissue repository > > Hi Curt, > > Does your lab have an in-house legal department? If so, have you > consulted with them about this? The attorneys for the hospital I worked in > wanted nothing to do with providing patient tissue to outside interests who > were willing to pay for it. > > Richard Cartun > > On Wednesday, July 31, 2024 at 05:02:57 PM EDT, Curt Tague via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi all, > > So running a ref lab for years I've acquired a rather large supply of old > blocks of various tissues, I'm considering turning this into a tissue > bank/repository with the purpose of selling blocks for research and control > purposes... anyone have any experience, what are the requirements to be > compliant??? I have zero experience with this. I'm guessing some release > forms, maybe patient consent forms (IMPOSSIBLE TO GET THOSE FOR BLOCKS THAT > ARE 10 YRS OLD)... release of liability forms... I'm starting at zero so > any insight is appreciated! > > Best! > > Curt > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet< > https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwMFaQ&c=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM&r=2t7_gY_QgCsQnEhIbW0hGqXohzrIjiJRpXu4w5m7YEQ&m=JFfG7LfX5Aox_H49afQM7lStvCjkqj80OHLlP4WkOuSUjz6eGyXp5-nqhUbjtaxH&s=bBdY3ntKhWN79UmtmLaJ3e2ylDnuah9MlCcGLW-EqFc&e= > > > > > > > ---------- Forwarded message ---------- > From: > To: > Cc: > Bcc: > Date: Thu, 1 Aug 2024 12:25:55 -0400 > Subject: [Histonet] Happy Thursday Histonetters!! > Happy Thursday, Histopeeps! ? > > Can you believe it?s already August 1st? ? > > ICYMI - Throwback Thursday: If you missed out on the RELIA Histopeep > Summer Splash 2024, there?s still time to join us! > > Dive into: > > Histology Art ? > > Resume Tune-Ups ? > > Histology Haiku ?? > > Blog Posts ? > > Career Advice ? > > Fantastic Job Opportunities ? > > Reach out to me: > > Message me on social media ? > > Email: relia1 at earthlink.net ? > > Call/Text: 407-353-5070 ? > > Check out the hashtag: #RELIASummerSplash2024 ? > > Catch up and have a fantastic day! ? > > Thanks, Pam > > #Thursday #ThrowbackThursday #RELIASummerSplash2024 #Ilovemyhistopeeps > #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs > #histologycareers > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcokertx at gmail.com Thu Aug 1 19:10:52 2024 From: mcokertx at gmail.com (Michelle Bell) Date: Fri, 2 Aug 2024 00:10:52 +0000 Subject: [Histonet] IHC help In-Reply-To: References: Message-ID: If you?re doing online retrieval/deparaffinization, try deparafinizing offline. I found the paraffin we loved had was higher in polymers and resulted in the tissue being prone to lifting. As soon as we started deparafinizing BEFORE putting the slides on the instrument, we had greater (not perfect, mind you) success. ________________________________ From: Renee Fisher via Histonet Sent: Thursday, August 1, 2024 2:39:01 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC help Does anyone have a technique that will keep mice skin tissue on the slide through the retrieval process. We are using positive charged slides, but still have tissue lifting. We do digital imaging and AI the lifting of the tissue affects the AI. Get Outlook for iOS _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs at kcl.ac.uk Fri Aug 2 13:21:39 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Fri, 2 Aug 2024 18:21:39 +0000 Subject: [Histonet] IHC help Message-ID: Hi I have same problem with Pwax Ms/rat skin, ear, knee joint sections I always lose the bone/cartilage but......everything else is retained by using HIER at 90C for a time optimised by your lab Sure, collagen does NOT like HIER You know this already, I am sure Maybe your abs will work on alternative AR? eg: Proteinase K? This will NOT disrupt tissue sections, provided you don't over-digest I have posted sensitivity comparisons on Histonet images...I think....giving anti GFP as an example Good luck ? Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From akemiat3377 at gmail.com Fri Aug 2 15:19:45 2024 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Fri, 2 Aug 2024 13:19:45 -0700 Subject: [Histonet] Bill DeSalvo and ASSIST Tracking Message-ID: <7A40235C-5DAF-4A59-8F18-5F4E94235078@gmail.com> Hi Histo Land: Happy TGIF! I have a quick question; I am trying to get a hold of Bill DeSalvo. I wanted to ask him about interfacing ASSIST Tracking Software with our IntelliPath LIS. I called his old cell number I had from over 15 years ago, but I think it isn?t any good any more. Anybody want to share his current contact information? Not sure if he is working at Quest Sonora anymore, or he retired. If any of you in Histoland are using these systems and want to chime in, please do. Have a great weekend! Best regards, Akemi Akemi Allison-Tacha BS, HT/HTL (ASCP) Laboratory Manager at Golden State Dermatology 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 Cell: (408) 335-9994 Email: aallison at gsdermca.com Website: www.goldenstatedermatology.com From relia1 at earthlink.net Mon Aug 5 12:01:12 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 5 Aug 2024 13:01:12 -0400 Subject: [Histonet] New Blog Post UP! From Great to Greatest: How to Select The Best Histotech Among TOP Candidates. Message-ID: <001901dae759$14d7bf00$3e873d00$@earthlink.net> Hey Histopeeps! New Blog Alert! Struggling with Histology Hiring Challenges? I?ve got you covered! Check out my latest blog post on selecting the best histotech among top candidates. * Read it here: * https://reliasolutionspambarker.wordpress.com/2024/08/05/from-great-to-great est-how-to-select-the-best-histotech-among-top-candidates/ Need a GREAT tech? I?m here to help! Contact Me: Pam Barker #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From PKRichar at gundersenhealth.org Tue Aug 6 11:24:22 2024 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Tue, 6 Aug 2024 16:24:22 +0000 Subject: [Histonet] Kimwipes In-Reply-To: References: Message-ID: With Kimwipes being discontinued has anyone found a suitable replacement? Best Wishes Pam Richardson Clinical Manager Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen Health System? We're becoming Emplify Health! Learn more at emplifyhealth.org. Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 +++++++++++++++++++++++++++ National Histology Professionals Day 3/10/2024 Pathologists' Assistant Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical Laboratory Professionals Week April 14-20, 2024 National Cytotechnology Day 5/13/2024 From paula at excaliburpathology.com Tue Aug 6 11:47:44 2024 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Tue, 6 Aug 2024 16:47:44 +0000 (UTC) Subject: [Histonet] Kimwipes In-Reply-To: References: Message-ID: <1240640227.3639493.1722962864694@mail.yahoo.com> I have been using Georgia Pacific - Pacific Blue Basic AccuWipe Disposable Delicate Task Wiper. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, August 6, 2024 at 11:31:11 AM CDT, Richardson, Pam K via Histonet wrote: With Kimwipes being discontinued has anyone found a suitable replacement? Best Wishes Pam Richardson Clinical Manager Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen Health System? We're becoming Emplify Health! Learn more at emplifyhealth.org. Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 +++++++++++++++++++++++++++ National Histology Professionals Day 3/10/2024 Pathologists' Assistant Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical Laboratory Professionals Week April 14-20, 2024 National Cytotechnology Day 5/13/2024 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jaylundgren at gmail.com Tue Aug 6 11:51:13 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 6 Aug 2024 11:51:13 -0500 Subject: [Histonet] Kimwipes In-Reply-To: References: Message-ID: NOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!! We used to use phonebook pages cut in half, worked better than kimwipes, but phonebooks are hard to find these days. I've worked at labs where they used tri-fold paper towels. Wasteful, but they work. Check Grainger? Or Statlab? I know there are other brands of industrial task wipes out there. Gotta prevent those floaters! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Aug 6, 2024 at 11:24?AM Richardson, Pam K via Histonet < histonet at lists.utsouthwestern.edu> wrote: > With Kimwipes being discontinued has anyone found a suitable replacement? > > Best Wishes > > Pam Richardson > Clinical Manager > Laboratory Anatomic Pathology and Microbiology > Bellin and Gundersen Health System > > We're becoming Emplify Health! Learn more at emplifyhealth.org. > > > > Email: pkrichar at gundersenhealth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > +++++++++++++++++++++++++++ > > National Histology Professionals Day 3/10/2024 > Pathologists' Assistant Day 4/14/2024 > Administrative Professionals Day 4/24/2024 > Medical Laboratory Professionals Week April 14-20, 2024 > National Cytotechnology Day 5/13/2024 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bcooper at chla.usc.edu Tue Aug 6 11:52:55 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 6 Aug 2024 16:52:55 +0000 Subject: [Histonet] Kimwipes In-Reply-To: <1240640227.3639493.1722962864694@mail.yahoo.com> References: <1240640227.3639493.1722962864694@mail.yahoo.com> Message-ID: We've been using TechWipes; we get them from Creative Waste Solutions. Hadn't heard that KimWipes were going away! Thanks, Brian -----Original Message----- From: Paula Keene Pierce via Histonet Sent: Tuesday, August 6, 2024 9:48 AM To: histonet at lists.utsouthwestern.edu; Richardson, Pam K Subject: Re: [Histonet] Kimwipes (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. I have been using Georgia Pacific - Pacific Blue Basic AccuWipe Disposable Delicate Task Wiper. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://http://secure-web.cisco.com/1HyLm5r1qvHqcH0RFKn2RyAuJR-nCGdH2ysqv7A9kksQN8rQq_bwup5RRofWSBloHKDigln3kikAZPF_Kb836XTfTk2MxuW0nlIuscSnBpe2rEdIjJOys64Pi9gvSIj6c1wEhhKSPhRNxWz9XyutBSuPXBwQep9OqJIx0nNUIag7ep1ef75F0zwHR8OJtDFnbstcnGxs_p1CFiVWtUyQRA_TWAmMfPGQQThhBLyWDE81D4sKdJ72zT8htRnsD7SAksSzAeU9erEnRsgWlXLD0rRk8mxmVU0005OfcrUbACOZxvR1khrgilczxLGOWLDqgdecX4_ni3nSqUdqsF8F-EovDFklZeNrHcVf_7uvjDHxkmDUI5hN5iHOCjYYIpWwpvQlDdL1VtUGGoVqf_odufjbfNmRq6RgZ26pLuHTnzNc4DbwR5NV3r0T09H5_m2xM/http%3A%2F%2Fwww.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Tuesday, August 6, 2024 at 11:31:11 AM CDT, Richardson, Pam K via Histonet wrote: With Kimwipes being discontinued has anyone found a suitable replacement? Best Wishes Pam Richardson Clinical Manager Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen Health System? We're becoming Emplify Health! Learn more at emplifyhealth.org. Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 +++++++++++++++++++++++++++ National Histology Professionals Day 3/10/2024 Pathologists' Assistant Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical Laboratory Professionals Week April 14-20, 2024 National Cytotechnology Day 5/13/2024 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1d7-7O4gDwqjvXutPnP8azC3ktLc8TCf4lvq2MW-qMg3nWwQgVYESkVFVADPHkydjyBc1ZfWghhI6pHZ9EzJkQ1l8W7K-ct5I39-0lSbYDF03bJMNJoRyZp1qrxFcwDKe3AUnIyTFOHnTPVDuJoTOyt989Xe706wO7TEHCgX9vpU0JWsS_cu-dbKd8wGa7bHdf8O87HybWIsUbkS8LPCwVX10Dj1zGTioMPW5CL2nDuaiD4SXAIKPJOjaujhXg5apzfhVG7TgQTfOn8aQwEV3IC69vcs1IhTXWDFMFiWs6Pv3rEsaXOhkpSTuFJgGzRFkXJmjRJ7HyaGV_UmmOJiab0JJUCTiC0YhzZ_eJx12CW8RtMb7J2hJTSCJxzo8CkbTmUnGuZL3xkVdA_JeXiN4lJF0y9KUepMiw25UKBjGcQ3kp1ccrQyLINTmFLyzcAtw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1d7-7O4gDwqjvXutPnP8azC3ktLc8TCf4lvq2MW-qMg3nWwQgVYESkVFVADPHkydjyBc1ZfWghhI6pHZ9EzJkQ1l8W7K-ct5I39-0lSbYDF03bJMNJoRyZp1qrxFcwDKe3AUnIyTFOHnTPVDuJoTOyt989Xe706wO7TEHCgX9vpU0JWsS_cu-dbKd8wGa7bHdf8O87HybWIsUbkS8LPCwVX10Dj1zGTioMPW5CL2nDuaiD4SXAIKPJOjaujhXg5apzfhVG7TgQTfOn8aQwEV3IC69vcs1IhTXWDFMFiWs6Pv3rEsaXOhkpSTuFJgGzRFkXJmjRJ7HyaGV_UmmOJiab0JJUCTiC0YhzZ_eJx12CW8RtMb7J2hJTSCJxzo8CkbTmUnGuZL3xkVdA_JeXiN4lJF0y9KUepMiw25UKBjGcQ3kp1ccrQyLINTmFLyzcAtw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From PKRichar at gundersenhealth.org Tue Aug 6 12:48:18 2024 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Tue, 6 Aug 2024 17:48:18 +0000 Subject: [Histonet] Kimwipes update In-Reply-To: References: Message-ID: Thank you for your help. Kimwipes are not being discontinued, our supplier is no longer carrying them. Best Wishes Pam Richardson Clinical Manager Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen Health System? We're becoming Emplify Health! Learn more at emplifyhealth.org. Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 +++++++++++++++++++++++++++ National Histology Professionals Day 3/10/2024 Pathologists' Assistant Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical Laboratory Professionals Week April 14-20, 2024 National Cytotechnology Day 5/13/2024 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, August 6, 2024 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 249, Issue 4 Attention: You have received this email from someone outside of Gundersen. Please use caution responding, opening attachments or clicking on links. Report this email using the "Report Phish" button in Outlook if you are uncertain about it, if it asks for an account validation, password reset, or claims to be a security alert. 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From Jessica.Piche at wtbyhosp.org Tue Aug 6 12:59:11 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 6 Aug 2024 17:59:11 +0000 Subject: [Histonet] Kimwipes update In-Reply-To: References: Message-ID: Whew, that is a relief!!!! ? I was taking notes or all the different suggestions and everything....love the KimWipe!!! Jessica Pich?, HT(ASCP) Histology Team Leader, Laboratory Waterbury Health 64 Robbins Street Waterbury, Connecticut, 06708 Phone: 203-573-7167 FAX: 203-573-7242 Email:jessica.piche at wtbyhosp.org Waterbury HEALTH ________________________________ From: Richardson, Pam K via Histonet Sent: Tuesday, August 6, 2024 1:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Kimwipes update [EXTERNAL MSG] Thank you for your help. Kimwipes are not being discontinued, our supplier is no longer carrying them. Best Wishes Pam Richardson Clinical Manager Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen Health System We're becoming Emplify Health! Learn more at emplifyhealth.org. Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 +++++++++++++++++++++++++++ National Histology Professionals Day 3/10/2024 Pathologists' Assistant Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical Laboratory Professionals Week April 14-20, 2024 National Cytotechnology Day 5/13/2024 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, August 6, 2024 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 249, Issue 4 Attention: You have received this email from someone outside of Gundersen. Please use caution responding, opening attachments or clicking on links. Report this email using the "Report Phish" button in Outlook if you are uncertain about it, if it asks for an account validation, password reset, or claims to be a security alert. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://secure-web.cisco.com/1RjD5s3dXUtunyCJ9Rlte20h3UNB3p1trZ8dUwGDuchCPSSKF1nUqdN6V5Qbb0yOv7vA8rysdDUYmb6At1HT6mKP3FPODhdEr1CtbVVp4VysvC3s0zjHyQ2_gu7le6Q_UsKqARcRWpB5801HIQFyaW4dZzTzxu5_koABpr0-_IrRRLx23Fbyh0JVTbXqeHg0mBqnW8qIlGP4JB3or2qGZMwXWIyUWuaF1mLkwbAscvDiUlRmsX97CKAubPmO1ybM7WP_JnQYQLvDoqf_OgEB2kFiRAcceruPJ0wuq2VGFharwRX66Qjakh54JI7RDZLmrkpJW8FJRBTI9EOTTH0853A/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Piche at wtbyhosp.org Tue Aug 6 13:02:17 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 6 Aug 2024 18:02:17 +0000 Subject: [Histonet] Charged slides Message-ID: Hello Everyone, Does anyone have any suggestions for charged slides that don't have the x or + at the bottom of the slide? We do not love them for FNA smears, etc. I am having a hard time finding any. Thank you, Jessica Jessica Pich?, HT(ASCP) Histology Team Leader, Laboratory Waterbury Health 64 Robbins Street Waterbury, Connecticut, 06708 Phone: 203-573-7167 FAX: 203-573-7242 Email:jessica.piche at wtbyhosp.org Waterbury HEALTH From jmacdonald at mtsac.edu Tue Aug 6 18:31:46 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 6 Aug 2024 23:31:46 +0000 Subject: [Histonet] Kimwipes In-Reply-To: References: Message-ID: I tell my students about using phone book pages. They look at me in doubt. -----Original Message----- From: Jay Lundgren via Histonet Sent: Tuesday, August 6, 2024 9:51 AM To: Richardson, Pam K Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Kimwipes EXTERNAL SENDER - Exercise caution with requests, links, and attachments. NOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!! We used to use phonebook pages cut in half, worked better than kimwipes, but phonebooks are hard to find these days. I've worked at labs where they used tri-fold paper towels. Wasteful, but they work. Check Grainger? Or Statlab? I know there are other brands of industrial task wipes out there. Gotta prevent those floaters! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Aug 6, 2024 at 11:24?AM Richardson, Pam K via Histonet < histonet at lists.utsouthwestern.edu> wrote: > With Kimwipes being discontinued has anyone found a suitable replacement? > > Best Wishes > > Pam Richardson > Clinical Manager > Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen > Health System > > We're becoming Emplify Health! Learn more at emplifyhealth.org. > > > > Email: pkrichar at gundersenhealth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > +++++++++++++++++++++++++++ > > National Histology Professionals Day 3/10/2024 Pathologists' Assistant > Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical > Laboratory Professionals Week April 14-20, 2024 National > Cytotechnology Day 5/13/2024 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists/ > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjma > cdonald%40mtsac.edu%7Cae16a7202a154c84705408dcb63811b3%7Ccc4d4bf20a9e4 > 240aedea7d1d688f935%7C0%7C0%7C638585599133394769%7CUnknown%7CTWFpbGZsb > 3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D% > 7C0%7C%7C%7C&sdata=PuxfOSldjP7b44ejXDMDCtn0lUC8EiV%2BPCdeRqZrv4c%3D&re > served=0 > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eddessa at emory.edu Tue Aug 6 19:09:51 2024 From: eddessa at emory.edu (Dessasau, Evan D.) Date: Wed, 7 Aug 2024 00:09:51 +0000 Subject: [Histonet] [External] Re: Kimwipes In-Reply-To: References: Message-ID: What about VWR Light Duty Tissue Wipers 82003-820. This is what we have on hand at the moment. Very similar size box to Kimwipes. Also, PathSUPPLY has a similar product. Thank you, E-van //////// Emory Univ. EPC Histology 2122A 954 Gatewood Rd., Atlanta,Ga. 30329 404-727-7744(on campus 7-7744) Lab -----Original Message----- From: Mac Donald, Jennifer via Histonet Sent: Tuesday, August 6, 2024 7:32 PM To: Jay Lundgren ; Richardson, Pam K Cc: histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Kimwipes I tell my students about using phone book pages. They look at me in doubt. -----Original Message----- From: Jay Lundgren via Histonet Sent: Tuesday, August 6, 2024 9:51 AM To: Richardson, Pam K Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Kimwipes EXTERNAL SENDER - Exercise caution with requests, links, and attachments. NOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!! We used to use phonebook pages cut in half, worked better than kimwipes, but phonebooks are hard to find these days. I've worked at labs where they used tri-fold paper towels. Wasteful, but they work. Check Grainger? Or Statlab? I know there are other brands of industrial task wipes out there. Gotta prevent those floaters! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Tue, Aug 6, 2024 at 11:24?AM Richardson, Pam K via Histonet < histonet at lists.utsouthwestern.edu> wrote: > With Kimwipes being discontinued has anyone found a suitable replacement? > > Best Wishes > > Pam Richardson > Clinical Manager > Laboratory Anatomic Pathology and Microbiology Bellin and Gundersen > Health System > > We're becoming Emplify Health! Learn more at emplifyhealth.org. > > > > Email: pkrichar at gundersenhealth.org > Phone: 608 775-4133 > Fax: 608 775-6136 > Interdepartmental Mail Stop: H04-007 > +++++++++++++++++++++++++++ > > National Histology Professionals Day 3/10/2024 Pathologists' Assistant > Day 4/14/2024 Administrative Professionals Day 4/24/2024 Medical > Laboratory Professionals Week April 14-20, 2024 National > Cytotechnology Day 5/13/2024 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists/ > .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjma > cdonald%40mtsac.edu%7Cae16a7202a154c84705408dcb63811b3%7Ccc4d4bf20a9e4 > 240aedea7d1d688f935%7C0%7C0%7C638585599133394769%7CUnknown%7CTWFpbGZsb > 3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D% > 7C0%7C%7C%7C&sdata=PuxfOSldjP7b44ejXDMDCtn0lUC8EiV%2BPCdeRqZrv4c%3D&re > served=0 > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.a.heyward at odont.uio.no Thu Aug 8 07:10:11 2024 From: c.a.heyward at odont.uio.no (Catherine Anne Heyward) Date: Thu, 8 Aug 2024 12:10:11 +0000 Subject: [Histonet] Re IHC help In-Reply-To: References: Message-ID: Hi, If HIER at 90 degrees C is causing tissue lifting, you could try a lower temperature for longer. I was recommended 60 degrees overnight for bone/cartilage and have had far fewer problems with this gentler treatment. Perhaps this would suit the skin samples too. Best wishes, Cath Catherine Heyward Ph.D Senior technician - Oral Research Laboratory Local senior health and safety representative - Faculty for Dentistry Tel: +47 9009 3113 Oral Research Laboratory Institute for Clinical Dentistry Postbox 1109 Blindern 0317 Oslo Geitmyrsveien 69/71 0455 Oslo From Robyn.L.Vazquez at kp.org Thu Aug 8 08:24:21 2024 From: Robyn.L.Vazquez at kp.org (Robyn L Vazquez) Date: Thu, 8 Aug 2024 13:24:21 +0000 Subject: [Histonet] Re IHC help In-Reply-To: References: Message-ID: Hello, Another part of the process is to wick the tissue and place in front of a fan for about 10mins before placing in the 60 degrees oven. Just a suggestion. Robyn -----Original Message----- From: Catherine Anne Heyward via Histonet Sent: Thursday, August 8, 2024 5:10 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Re IHC help Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ Hi, If HIER at 90 degrees C is causing tissue lifting, you could try a lower temperature for longer. I was recommended 60 degrees overnight for bone/cartilage and have had far fewer problems with this gentler treatment. Perhaps this would suit the skin samples too. Best wishes, Cath Catherine Heyward Ph.D Senior technician - Oral Research Laboratory Local senior health and safety representative - Faculty for Dentistry Tel: +47 9009 3113 Oral Research Laboratory Institute for Clinical Dentistry Postbox 1109 Blindern 0317 Oslo Geitmyrsveien 69/71 0455 Oslo _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!uluFqOasxhMtFqcdQizIWWk3gEV5N-vhhkx-nNFSSY2AuvGV5-cKJhkZfAyZsyMJWRekG8-wVKadQf2eThGlF5vTOY_d9e5cjTWt$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. From akemiat3377 at gmail.com Thu Aug 8 11:22:43 2024 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 8 Aug 2024 09:22:43 -0700 Subject: [Histonet] Oven Temps and baking times used for IHC's Message-ID: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> Good morning Histo Peeps! I am taking a poll on what various labs are using for temperature and times for IHC?s slides which are baked in the oven. It has been my experience while working at Biocare Medical, we would 1st wick excess water off the slides, then tap the rack of slides on paper; then subsequently place the rack of slides in a 36 degree oven for 30 minutes, then up the temperature to 60-62 degrees for an additional time of 30-60 minutes. Sorry, my brain cells can?t remember which length of time in 60 degrees, I left Biocare Medical in 2006. My ex, David Tacha, Ph.D, HT/HTL gave several workshops on this subject internationally. The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes their IHC slides at 62 degrees for 2 hours. I have consulted w/ some labs who bake their IHC slides at 76 degrees for 20 minutes. It has been my experience that higher than 62 degree temps will make the Ab lose their antigenicity. CK Ab?s love a higher temp but most other Ab?s do not. Any thoughts, information, and papers on this subject would be greatly appreciated. Thank you and have a wonderful upcoming weekend! Best regards, Akemi Akemi Allison-Tacha BS, HT/HTL (ASCP) Pathology Laboratory Manager at Golden State Dermatology 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 Cell: (408) 335-9994 Email: aallison at gsdermca.com Website: www.goldenstatedermatology.com From nmargaryan88 at gmail.com Thu Aug 8 19:36:59 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Thu, 8 Aug 2024 19:36:59 -0500 Subject: [Histonet] Oven Temps and baking times used for IHC's In-Reply-To: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> References: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> Message-ID: Interesting. I never baked my slides. Left air dry o/n on room. Next day started with deparaffinization steps 2x30min. My most AR where in the citrate buffer in the water bath with 92C(if I remember correctly) for 37 minutes then 12 minutes cooling at room temperature. Never had a problem. Naira On Thu, Aug 8, 2024 at 11:28?AM Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning Histo Peeps! > > I am taking a poll on what various labs are using for temperature and > times for IHC?s slides which are baked in the oven. It has been my > experience while working at Biocare Medical, we would 1st wick excess water > off the slides, then tap the rack of slides on paper; then subsequently > place the rack of slides in a 36 degree oven for 30 minutes, then up the > temperature to 60-62 degrees for an additional time of 30-60 minutes. > Sorry, my brain cells can?t remember which length of time in 60 degrees, I > left Biocare Medical in 2006. My ex, David Tacha, Ph.D, HT/HTL gave > several workshops on this subject internationally. > > The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes > their IHC slides at 62 degrees for 2 hours. I have consulted w/ some labs > who bake their IHC slides at 76 degrees for 20 minutes. It has been my > experience that higher than 62 degree temps will make the Ab lose their > antigenicity. CK Ab?s love a higher temp but most other Ab?s do not. > > Any thoughts, information, and papers on this subject would be greatly > appreciated. Thank you and have a wonderful upcoming weekend! > > Best regards, > Akemi > > Akemi Allison-Tacha BS, HT/HTL (ASCP) > Pathology Laboratory Manager at Golden State Dermatology > 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 > Cell: (408) 335-9994 > Email: aallison at gsdermca.com > Website: www.goldenstatedermatology.com < > http://www.goldenstatedermatology.com/> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From bcooper at chla.usc.edu Thu Aug 8 19:53:48 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Fri, 9 Aug 2024 00:53:48 +0000 Subject: [Histonet] Oven Temps and baking times used for IHC's In-Reply-To: References: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> Message-ID: We wick our sections dry and then incubate for 15 to 20 minutes @ 70-75 degrees in a convection oven. We tried lower temps and longer baking times for many of our markers, but we didn't see any discernable staining difference. Thank you, Brian Cooper, HT, QIHC (ASCP) Histology/EM Supervisor Children's Hospital Los Angeles 323-361-3357 bcooper at chla.usc.edu Sent from my mobile ________________________________ From: Naira Margaryan via Histonet Sent: Thursday, August 8, 2024 5:36:59 PM To: Eileen Akemi Allison Cc: Histonet Subject: Re: [Histonet] Oven Temps and baking times used for IHC's (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Interesting. I never baked my slides. Left air dry o/n on room. Next day started with deparaffinization steps 2x30min. My most AR where in the citrate buffer in the water bath with 92C(if I remember correctly) for 37 minutes then 12 minutes cooling at room temperature. Never had a problem. Naira On Thu, Aug 8, 2024 at 11:28?AM Eileen Akemi Allison via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good morning Histo Peeps! > > I am taking a poll on what various labs are using for temperature and > times for IHC's slides which are baked in the oven. It has been my > experience while working at Biocare Medical, we would 1st wick excess water > off the slides, then tap the rack of slides on paper; then subsequently > place the rack of slides in a 36 degree oven for 30 minutes, then up the > temperature to 60-62 degrees for an additional time of 30-60 minutes. > Sorry, my brain cells can't remember which length of time in 60 degrees, I > left Biocare Medical in 2006. My ex, David Tacha, Ph.D, HT/HTL gave > several workshops on this subject internationally. > > The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes > their IHC slides at 62 degrees for 2 hours. I have consulted w/ some labs > who bake their IHC slides at 76 degrees for 20 minutes. It has been my > experience that higher than 62 degree temps will make the Ab lose their > antigenicity. CK Ab's love a higher temp but most other Ab's do not. > > Any thoughts, information, and papers on this subject would be greatly > appreciated. Thank you and have a wonderful upcoming weekend! > > Best regards, > Akemi > > Akemi Allison-Tacha BS, HT/HTL (ASCP) > Pathology Laboratory Manager at Golden State Dermatology > 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 > Cell: (408) 335-9994 > Email: aallison at gsdermca.com > Website: http://secure-web.cisco.com/1c2QmUrfZNr8Bk_dX1wcdt15_RG3XovSgOjHunQHzv9ZGKJFRCOVCOGitmP_p9KKQvtWfViacz0Y4a6mHflg3ff9hBhJX09HFCqosEu4Vp_Nn5R61lyqlBoKFws1kLdO4wv62JI65Y23YgvTn53yrRobTnhaE7utyvsSezBtyURZKMQ7yqRWpWote12HKwzQyFvfAMYbDirup1F8AjntLKg9bbP3RB6qnlbXKNgdOnyAq61gaxSSWVJE6qac1PTm_R2_4ERYjiVNQ4TP9lE6Ftnc-Omh1s6xehplfsq9cRBfm2ERST2RUYd2-n9Gcr9x4bKIIHw0dm6xyjTIX96hjAu6RGxn8ZEWez5Aj0hgyFx9FlTQgfZ25RRmzpyuP_l8pXv0rBxvEvuBoaKvbSEWo6Ni4ul1RJOcucw0mxwU-AfikLk11X_peTgcLpGqFK-G9/http%3A%2F%2Fwww.goldenstatedermatology.com < > http://secure-web.cisco.com/1qqZEgTRgnSk-m7eOKE8Mb09khGqTkT6q4XiPpY6z1XZ4RGCg_aG4kUhX0mC4jEq7Nb9KyA_gJmfO7zBugTvWhN2xnQFuoaH4GP93p7j9s0UkTX4_h3spvBHaC10bb7s79w6gNvLYvnPzTW-b0tqfV4lbfKw2XVwXt1p0yPYONO7uRqnsXMZpcTQQK05-mPWO4rOaEbdG1O0JDjFSNXrt3OXiVFzZD1Zi2tD75OtYV73QL0A_1j2aM6fQH5U0eZjJQMMQAw9pmTO8O2kSTuo1rX0bITgDWpz6DTcAZ6CDS5BNUjjGzT63LIC27_nBR9QGEwWbv9lu8LJyPFD0f0Mi8mxjYWjjdI2NKtniSZVenno_d3iWt3EtLNzj4wDVlOhc9karp32QBV4cvodXvVGPIE0oc35HqIE7quYi9sUUqW1ov6XIkh-8LsM8m0eikD_s/http%3A%2F%2Fwww.goldenstatedermatology.com%2F> > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://secure-web.cisco.com/1PE2q0-i3FjHb2WHqMAgBhuoM-oCsLrbndWUPcbyvs-kDBoR4pCju7UNRg0gPtmaYBmTtf7ZVM0iCT40FzRzvyBZD30Uj8HrTsfV9Rp57knXr_cXSz-6RzhX_wHC96N3JDyG74DpOxCGz56Pccneq93C8r2hqP2GdAlgxbXrryB9U1lbQ4K4qKvSsyvZWBLeg7pHqRTXx6qu-GgJ9tlsNNlmV7VLn94QkWPlW8sboYlE1KaQG7z7QOgysNdEmsX-3GeLXgqy5o0b6roS7PfxRnqY9eX2bRGO5dxqG9w52DOSKZkKhkoCzY0g8VfZDhkw2xLAPBJgvj0eTb8UeDJ9SkwU-TsfGbi0eoEalbiy6T37SyZeSGvEYFAsl7Z2d41tAcwnA_yOsSwo7Mt2PnoxmsTHcIMVmjW4X6OOoBGZ7eNKbj1ZvMRbvJBEY_EcPSYcgHhaUN2BQW-PinZKri60Nzw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1PE2q0-i3FjHb2WHqMAgBhuoM-oCsLrbndWUPcbyvs-kDBoR4pCju7UNRg0gPtmaYBmTtf7ZVM0iCT40FzRzvyBZD30Uj8HrTsfV9Rp57knXr_cXSz-6RzhX_wHC96N3JDyG74DpOxCGz56Pccneq93C8r2hqP2GdAlgxbXrryB9U1lbQ4K4qKvSsyvZWBLeg7pHqRTXx6qu-GgJ9tlsNNlmV7VLn94QkWPlW8sboYlE1KaQG7z7QOgysNdEmsX-3GeLXgqy5o0b6roS7PfxRnqY9eX2bRGO5dxqG9w52DOSKZkKhkoCzY0g8VfZDhkw2xLAPBJgvj0eTb8UeDJ9SkwU-TsfGbi0eoEalbiy6T37SyZeSGvEYFAsl7Z2d41tAcwnA_yOsSwo7Mt2PnoxmsTHcIMVmjW4X6OOoBGZ7eNKbj1ZvMRbvJBEY_EcPSYcgHhaUN2BQW-PinZKri60Nzw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From gu.lang at gmx.at Fri Aug 9 02:44:03 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 9 Aug 2024 09:44:03 +0200 Subject: [Histonet] Oven Temps and baking times used for IHC's In-Reply-To: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> References: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> Message-ID: <000901daea2f$e8703080$b9509180$@gmx.at> Hallo! Sections are dried at room-temperature vertically to get rid of the water beneath the sections. For automated staining they aren't baked any more. For semi-manual staining with HIER in the pressure cooker they were baked at 60?C for 30-60 min. Dry temperatures above 70?C influence the results of IHC. Williams JH, Mepham BL, Wright DH (1997) Tissuepreparation for immunocytochemistry J Clin Pathol 50:422?428 Gudrun Lang Kepleruniversit?tsklinik Linz, Austria -----Urspr?ngliche Nachricht----- Von: Eileen Akemi Allison via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 8. August 2024 18:23 An: Histonet Betreff: [Histonet] Oven Temps and baking times used for IHC's Good morning Histo Peeps! I am taking a poll on what various labs are using for temperature and times for IHC?s slides which are baked in the oven. It has been my experience while working at Biocare Medical, we would 1st wick excess water off the slides, then tap the rack of slides on paper; then subsequently place the rack of slides in a 36 degree oven for 30 minutes, then up the temperature to 60-62 degrees for an additional time of 30-60 minutes. Sorry, my brain cells can?t remember which length of time in 60 degrees, I left Biocare Medical in 2006. My ex, David Tacha, Ph.D, HT/HTL gave several workshops on this subject internationally. The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes their IHC slides at 62 degrees for 2 hours. I have consulted w/ some labs who bake their IHC slides at 76 degrees for 20 minutes. It has been my experience that higher than 62 degree temps will make the Ab lose their antigenicity. CK Ab?s love a higher temp but most other Ab?s do not. Any thoughts, information, and papers on this subject would be greatly appreciated. Thank you and have a wonderful upcoming weekend! Best regards, Akemi Akemi Allison-Tacha BS, HT/HTL (ASCP) Pathology Laboratory Manager at Golden State Dermatology 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 Cell: (408) 335-9994 Email: aallison at gsdermca.com Website: www.goldenstatedermatology.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Schieberl at commonspirit.org Fri Aug 9 07:51:34 2024 From: Karen.Schieberl at commonspirit.org (Karen Schieberl CA-San Francisco) Date: Fri, 9 Aug 2024 05:51:34 -0700 Subject: [Histonet] Oven Temps and baking times used for IHC's In-Reply-To: <000901daea2f$e8703080$b9509180$@gmx.at> References: <3C266496-B0D3-474E-9B35-8DACBE6C7A79@gmail.com> <000901daea2f$e8703080$b9509180$@gmx.at> Message-ID: I tap and pat my slides dry on a Kimwipe. Place them n my Gemini Autostainer which has an onboard heater. I heat the slides for 20 minutes and then they get deparaffinized. Then I proceed with HIER if needed and then on to the Intellipath. Never had an issue, NEW EMAIL:Karen.Schieberl at UCSF.edu Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org NEW EMAIL:Karen.Schieberl at UCSF.edu Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Fri, Aug 9, 2024 at 12:44?AM Gudrun Lang via Histonet < histonet at lists.utsouthwestern.edu> wrote: > USE CAUTION - EXTERNAL EMAIL > > ...................................................................... > Hallo! > Sections are dried at room-temperature vertically to get rid of the water > beneath the sections. For automated staining they aren't baked any more. > For semi-manual staining with HIER in the pressure cooker they were baked > at 60?C for 30-60 min. > > Dry temperatures above 70?C influence the results of IHC. > Williams JH, Mepham BL, Wright DH (1997) Tissuepreparation for > immunocytochemistry J Clin Pathol 50:422?428 > > Gudrun Lang > Kepleruniversit?tsklinik Linz, Austria > > -----Urspr?ngliche Nachricht----- > Von: Eileen Akemi Allison via Histonet [mailto: > histonet at lists.utsouthwestern.edu] > Gesendet: Donnerstag, 8. August 2024 18:23 > An: Histonet > Betreff: [Histonet] Oven Temps and baking times used for IHC's > > Good morning Histo Peeps! > > I am taking a poll on what various labs are using for temperature and > times for IHC?s slides which are baked in the oven. It has been my > experience while working at Biocare Medical, we would 1st wick excess water > off the slides, then tap the rack of slides on paper; then subsequently > place the rack of slides in a 36 degree oven for 30 minutes, then up the > temperature to 60-62 degrees for an additional time of 30-60 minutes. > Sorry, my brain cells can?t remember which length of time in 60 degrees, I > left Biocare Medical in 2006. My ex, David Tacha, Ph.D, HT/HTL gave > several workshops on this subject internationally. > > The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes > their IHC slides at 62 degrees for 2 hours. I have consulted w/ some labs > who bake their IHC slides at 76 degrees for 20 minutes. It has been my > experience that higher than 62 degree temps will make the Ab lose their > antigenicity. CK Ab?s love a higher temp but most other Ab?s do not. > > Any thoughts, information, and papers on this subject would be greatly > appreciated. Thank you and have a wonderful upcoming weekend! > > Best regards, > Akemi > > Akemi Allison-Tacha BS, HT/HTL (ASCP) > Pathology Laboratory Manager at Golden State Dermatology > 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 > Cell: (408) 335-9994 > Email: aallison at gsdermca.com > Website: > https://urldefense.com/v3/__http://www.goldenstatedermatology.com__;!!CqLityr3mSQ!GtA3HJeb3ubrs0dJExQ3QO06oguerC0OqF_MpfH4J560Uz79tOThzFX4RufC3TLF7LvZ3Kba9FgSncKO5Zv-DZIK-jhgxi5QiQ$ > < > https://urldefense.com/v3/__http://www.goldenstatedermatology.com/__;!!CqLityr3mSQ!GtA3HJeb3ubrs0dJExQ3QO06oguerC0OqF_MpfH4J560Uz79tOThzFX4RufC3TLF7LvZ3Kba9FgSncKO5Zv-DZIK-jjg6yuZ7w$ > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GtA3HJeb3ubrs0dJExQ3QO06oguerC0OqF_MpfH4J560Uz79tOThzFX4RufC3TLF7LvZ3Kba9FgSncKO5Zv-DZIK-jjUGLGqLw$ > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GtA3HJeb3ubrs0dJExQ3QO06oguerC0OqF_MpfH4J560Uz79tOThzFX4RufC3TLF7LvZ3Kba9FgSncKO5Zv-DZIK-jjUGLGqLw$ > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From relia1 at earthlink.net Fri Aug 9 11:10:23 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 9 Aug 2024 12:10:23 -0400 Subject: [Histonet] Happy Friday Histopeeps! I Have Some Exciting News! Message-ID: <01ec01daea76$a44a8a80$ecdf9f80$@earthlink.net> Happy Friday, Histopeeps! ? I?ve got some incredible RELIA EXCLUSIVE Histology opportunities just for you! These Positions Are Unique To Us?You Won?t Find Them With Any Other Recruiters! What?s On Offer? VERY Competitive Compensation Packages ? Relocation Assistance ? Sign-On Bonuses Up To $20K! ? These Are Full-Time, Permanent Roles, And While There Are Many Opportunities For Histotechs Right Now? These Are Truly Special! HERE THEY ARE: WA - Washington: Histology Supervisor ? 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Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From tora.bardal at ntnu.no Mon Aug 12 06:18:26 2024 From: tora.bardal at ntnu.no (Tora Bardal) Date: Mon, 12 Aug 2024 11:18:26 +0000 Subject: [Histonet] toluidin blue Message-ID: To all the good histochemist/ chemists ... I usually make up toluidine blue for staining Epon and Technovit in borate buffer, 1 % konc. Due to HSE I'm interested in substituting the sodium borate buffer with another buffer. Any knowledge out there?? Recommendations? Tora Tora Bardal Senior Engineer NTNU Sealab 7491 Trondheim Tlf: 73 59 09 38 Mobil: 970 25256 Vare- og bes?ksadresse: NTNU Sealab Bratt?rkaia 17C 7010 Trondheim From jaylundgren at gmail.com Mon Aug 12 18:50:58 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Mon, 12 Aug 2024 18:50:58 -0500 Subject: [Histonet] toluidin blue In-Reply-To: References: Message-ID: IMHO, Borax ain't that dangerous, my Granny put it in her laundry. However... Toluidine Blue is basic, it stains acidic tissue components. It will stain differently at different pHs. It gives increased metachromatic staining at pH 4. What pH buffer system are you looking for? Sodium phosphate, monobasic 4.0 gm, Sodium phosphate, dibasic 6.5 gm, qs to 1L with diH2O gives pH 6.8. Ammonia solution and ammonium chloride in equal molar concentration gives you pH 9.25. Though if they won't let you play with borax, they probably won't let you play with ammonia. Ethanoic acid and sodium ethanoate in equal molar concentration gives you pH 4.76. They probably don't want you handling glacial acetic acid either. If you want to get fancy, make McIlvaine buffer, any pH you want from 2.2 to 8 by mixing 2 stock solutions of disodium phosphate and citric acid. You could do a range, and let the pathologist pick their favorite staining. Both are common food additives, so safe, unless you rub them in your eyes. Wikipedia has a table. I don't have to tell you that for any new protocol, you should run 15-20 independent controls. Cartilage or bone marrow is a good control for Toluidine Blue. Beware of moose, a moose once bit my sister, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Aug 12, 2024 at 6:26?AM Tora Bardal via Histonet < histonet at lists.utsouthwestern.edu> wrote: > To all the good histochemist/ chemists ... > > I usually make up toluidine blue for staining Epon and Technovit in borate > buffer, 1 % konc. > Due to HSE I'm interested in substituting the sodium borate buffer with > another buffer. > Any knowledge out there?? Recommendations? > > Tora > > Tora Bardal > Senior Engineer > NTNU Sealab > 7491 Trondheim > Tlf: 73 59 09 38 Mobil: 970 25256 > > Vare- og bes?ksadresse: > NTNU Sealab > Bratt?rkaia 17C > 7010 Trondheim > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From redrose297 at gmail.com Tue Aug 13 04:35:53 2024 From: redrose297 at gmail.com (warda hassan) Date: Tue, 13 Aug 2024 13:35:53 +0400 Subject: [Histonet] MDM2 Message-ID: Dear all We are using Dako Omnis, FISH MDM2, the template we are using built-in from the system. Unfortunately, after five hours ( as per Dako representative), our results are negative, including the commercial positive control from Bio SB. Can you please help in sharing any troubleshooting procedures for the above test? Thanking you in advance From SThompson4 at sonichealthcareusa.com Tue Aug 13 13:48:25 2024 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 13 Aug 2024 18:48:25 +0000 Subject: [Histonet] LAS VEGAS!!! Message-ID: <21a38ce9b0f943b6aa59f31f1fa9b609@sonichealthcareusa.com> Quality is in our DNA Quality is in our DNA -- is it in yours? You put the pro in medical laboratory professional. You've got problem-solving instincts, a passion for patient care, and the technical training to deliver quality results. You're also looking for great benefits, the support of an all-star team, and an opportunity to grow your career. Join our front line of #HealthcareHeroes! Our mission is to advance the health and wellbeing of our communities as a leader in clinical laboratory solutions. 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We'll give you: * Appreciation for your work * A feeling of satisfaction that you've helped people * Opportunity to grow in your profession * Free lab services for you and your dependents * Work-life balance, including Paid Time Off and Paid Holidays * Competitive benefits including medical, dental, and vision insurance * Help saving for retirement, with a 401(k) plus a company match * A sense of belonging - we're a community! https://shusa.wd5.myworkdayjobs.com/External/job/Las-Vegas-NV/Gross-Room-Technician---Las-Vegas--Nevada_REQ-026094 Contact: sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From SThompson4 at sonichealthcareusa.com Tue Aug 13 13:53:45 2024 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 13 Aug 2024 18:53:45 +0000 Subject: [Histonet] ***Competitive Sign On Bonus Offered*** Message-ID: <8464cf4518d34180bb965d9a8118926d@sonichealthcareusa.com> Sonic Healthcare USA ***Competitive Sign On Bonus Offered*** Location: Reno, Nevada Days: Monday - Friday Hours: 11:00 am - 7:30 pm Full-time: Benefit Eligible Responsibilities: * Prepares sections of human tissue for microscopic examination and patient diagnosis, using techniques to gross (dissect tissue) non-complex tissue specimens * May embed (orient specimen in paraffin block) * Section (cut thin sections of tissue), stain (enhance contrast of tissue and highlight specific features of interest with routine hematoxylin and eosin stains), and mount tissue (adhere tissue onto glass slides), from surgical procedures * Performs recuts and additional stains including manual special stain and automated immunohistochemistry stains * Operates and performs maintenance on laboratory equipment including tissue processors, embedding stations, automated H&E stainer and film coverslipper, microtomes, and IHC stainers Requirements: * Bachelor Degree in the field of Laboratory Science or equivalent under the 1988 Clinical Laboratory Improvement Amendments (CLIA) guidelines of high complexity testing. * 3+ years' full time grossing experience is preferred. * Eligible for State licensure * Completion of a Histology program accredited by the National Accrediting Agency for Clinical Laboratory Sciences (NAACLS) or minimum of one (1) year experience as a Histology Technician and competent in the areas of embedding, microtomy, grossing (preferred), special stains, and immunohistochemistry https://shusa.wd5.myworkdayjobs.com/External/job/Reno-NV/Histology-Technologist-Grossing---Reno--Nevada_REQ-025467 sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From relia1 at earthlink.net Wed Aug 14 12:04:47 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 14 Aug 2024 13:04:47 -0400 Subject: [Histonet] Seeking Autonomy in your Career? Run Your Own Lab, Let Your Specialties Shine and More!! Message-ID: <01fe01daee6c$13f9af20$3bed0d60$@earthlink.net> Hi Histonetters ?, Are you dreaming of having more autonomy in your career? Maybe you want to: ? Run Your Own Lab? ? Work in Vendor Support? ? Specialize in areas like IHC or Mohs? ? Take on a Leadership Role as a Lead Tech, Supervisor, or Manager? Here are my most exciting and newest roles: ? AL ? Alabama: Run Your Own Lab! ? WA ? Washington: Histology Supervisor ? VA ? Virginia: IHC Specialist ? FL ? Florida: AP Supervisor Mid-West: Lead Service Engineer Vendor Support ? I have fantastic roles for leadership and growth, including: ? Directors ? 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If you or any of your friends are interested or even curious about a new opportunity Contact Me! ? ? ? Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 That?s right, you might never see these amazing opportunities unless you read my emails because most of them are RELIA exclusives! ? Have a fantastic day! ? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From akemiat3377 at gmail.com Thu Aug 15 17:09:25 2024 From: akemiat3377 at gmail.com (Eileen Akemi Allison) Date: Thu, 15 Aug 2024 15:09:25 -0700 Subject: [Histonet] Validation form for Sakura Tape Coverslipper Message-ID: Hello out there HistoPeeps: I am reaching out to any of you who would be gracious enough to share your Validation Form for the Sakura Tape Coverslipper. I do not have one in my files and would be in debt to you for your kindness. We are getting a refurbished tape coverslipper in the next few days and I will need the Validation Form sooner than later. I know Sakura now has on their website ?Sakura University? which explains all the operations for their equipment, but I need a Validation form which they don?t have. Thank you in advance for your assistance, and have an awesome day! Best regards, Akemi Akemi Allison-Tacha BS, HT/HTL (ASCP) Pathology Laboratory Manager at Golden State Dermatology 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598 (925) 278-7592 Ext: 10079 Mobil:: (408) 335-9994 Email: aallison at gsdermca.com Website: www.goldenstatedermatology.com From relia1 at earthlink.net Tue Aug 20 11:14:58 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 20 Aug 2024 12:14:58 -0400 Subject: [Histonet] New Opportunities Comin In HOT!!! Check it OUT!! Message-ID: <006401daf31c$1aa75a20$4ff60e60$@earthlink.net> Hi Histopeeps! ?, I hope you?re having a fantastic day and an amazing start to your week! I am ? COMIN? IN HOT with new histology opportunities! ? These clients rarely have openings because they are such ? GREAT places to WORK! ? They offer excellent compensation, benefits, and opportunities to grow, learn, and advance! Maybe you want to: * Run Your Own Lab? * Work in Vendor Support? * Specialize in areas like IHC or Mohs? * Take on a Leadership Role as a Lead Tech, Supervisor, or Manager? Here Are My Most Exciting And Newest Roles: * AL ? Alabama: Run Your Own Lab! * WA ? Washington: Histology Supervisor * VA ? Virginia: IHC Specialist * FL ? Florida: AP Supervisor Mid-West: Lead Service Engineer Vendor Support ? I have fantastic roles for leadership and growth, including: * Directors * Managers * Supervisors * Lead Techs * IHC Specialist Most importantly, my clients are looking for Histotechs ready to learn, lead, grow, and prosper in: * Immunohistochemistry ? * Mohs ? * Dermatopathology ? * GI ? * CancerDx ? * Vendor Support ?? * We have opportunities nationwide, including in: * Florida ? * Alabama ? * Virginia ?? * Wisconsin ? * Illinois ? * Arizona ? * South Dakota ? * Montana ?? All of these opportunities are full-time and permanent. My clients offer outstanding compensation and benefits, including relocation and/or sign-on bonuses up to $20K! Histopeeps! I also have incredible technical and leadership opportunities COMIN? IN HOT daily! ? * ? My opportunities are with the best employers in histology! * ? I don?t work with big box anonymous labs; you can find those positions elsewhere. * ? RELIA?s positions are permanent with small to medium-sized labs that offer growth and opportunity. * ? I also collaborate with histology vendors and some research organizations. ? If you or any of your friends are interested or even curious about a new opportunity, Contact Me! ? ?? Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 You might never see these amazing opportunities unless you connect with ME! because most of them are RELIA exclusives! ? Have a fantastic day! ? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Wed Aug 21 11:56:19 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 21 Aug 2024 12:56:19 -0400 Subject: [Histonet] RELIA HOT Job Alert! Exciting Management Opportunity in Dallas, TX! Giddyy UP and Step UP? Message-ID: <026101daf3eb$0d6ca8e0$2845faa0$@earthlink.net> Hi Histonetters! I hope you?re having a fantastic day. ? I wanted to share an amazing opportunity with you! Why Giddy UP? Because it?s Dallas! ?? Why do I think it is amazing? This is the perfect chance to STEP UP! ? This Role Is Ideal For: * An experienced Histology Manager * An experienced Histology Supervisor ready for more! Histonetters!! I am working with a well-respected lab in Dallas, TX, known for its great reputation in the histology community. They are in need of a Histology Lab Manager. They seek an experienced Histology Manager or Supervisor ready for the next level, with strong clinical and leadership skills. They have a team of histotechs eager to continue their legacy of excellence with YOU! My client offers an excellent compensation package. If this sounds like the right job for you, RELIA can make it happen! For more information, please contact me, Pam Barker, at: E-mail: relia1 at earthlink.net Toll-free: 866-607-3542 Cell/Text: 407-353-5070 I also have new opportunities coming in daily nationwide, so if you are interested in another area or role, please reach out! ICYMI? If your team is shorthanded, you can earn a referral reward by connecting me with the hiring authority. Contact me, and I will fill you in on the details! Have a great day! Thanks, Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ? Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Thu Aug 22 12:08:08 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 22 Aug 2024 13:08:08 -0400 Subject: [Histonet] =?utf-8?b?8J+MnkhPVCBKb2JzIGluIEZMT1JJREEhIPCfjJ4=?= Message-ID: <017001daf4b5$dda113f0$98e33bd0$@earthlink.net> Hey Histopeeps!! Exciting NEW opportunities in Florida across multiple locations! ? Leadership and Tech positions available with excellent compensation, amazing benefits, and generous relocation assistance. What more could you ask for? * How About An Incredible Team To Work With, * Plenty Of Opportunities To Grow And Learn, * And Beautiful Communities To Call Home. These are Permanent Full-Time Positions! For more info, contact me: Message me on social media Email: relia1 at earthlink.net Cell/Text: 407-353-5070 #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobs4myhistopeeps #histologyjobs #histologycareers ?????? _____ Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Fri Aug 23 10:53:17 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 23 Aug 2024 11:53:17 -0400 Subject: [Histonet] We Made it Through The Dog Days of Summer Histopeeps! Message-ID: <019901daf574$99cd48f0$cd67dad0$@earthlink.net> Hi Histopeeps! ?? The Dog Days of Summer are here! But guess what? We made it through! As we set our sights on Labor Day, I hope you?re soaking up these last sunny days of summer. Have a fantastic weekend and stay tuned for some exciting updates next week! ??? ICYMI: I have exciting permanent positions nationwide! * My clients offer generous compensation packages and excellent, friendly teams to work with. * They aren?t understaffed because they know how to treat their techs right! ?? These opportunities are available due to: * Growth ? * New Labs ? * Retirements ? People stay at these labs because they are truly great places to work. If you?re even considering a job change between now and January 2025, we NEED to connect. How about a brief chat, text exchange, or email exchange? That?s ALL it takes to stay in the loop. ?? Contact Me! Direct Message me on Social Media Cell/Text: 407-353-5070 Email: relia1 at earthlink.net Let?s make the most of these dog days of summer together! Thanks - Pam ??????? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Tue Aug 27 12:52:48 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 27 Aug 2024 13:52:48 -0400 Subject: [Histonet] Summer is almost OVER! Free Resume Tune ups and Exciting and Exclusive Opportunities for you and your friends! Message-ID: <01b201daf8a9$eeb65860$cc230920$@earthlink.net> Hi Histopeeps ?, I hope you?re having a fantastic day and an amazing start to your week! The Summer is ALMOST over! Up NEXT ? Labor Day Weekend! The Last Of The Summer?s BBQ?s Beach Days And Getaways. As you enjoy the last of the Summer it may be the perfect time for me to Tune Up Your Resume! Resumes Are Not Just For Job Hunting. * Going For A Promotion? * Giving A Presentation * Volunteeering * Or A Great Way For You To See How Much You Have Accomplished. Shoot me an email to relia1 at earthlink.net with a copy of your resume. (preferably in Word) and I will take a look at it. Histopeeps! If you are curious about new opportunities or actively looking. Either way let me know so I can be on the lookout (BOLO) I am working with some amazing labs ? ? These clients rarely have openings because they are such ?? GREAT places to WORK! ? They offer excellent compensation, benefits, and opportunities to grow, learn, and advance! Maybe you want to: * Run Your Own Lab? * Work in Vendor Support? * Specialize in areas like IHC or Mohs? * Take on a Leadership Role as a Lead Tech, Supervisor, or Manager? ? I have fantastic roles for leadership and growth, including: * Directors * Managers * Supervisors * Lead Techs * IHC Specialist Most importantly, my clients are looking for Histotechs ready to learn, lead, grow, and prosper in: * Immunohistochemistry ? * Mohs ? * Dermatopathology ? * GI ? * CancerDx ? * Vendor Support ?? We have opportunities nationwide, including in: * Florida ? * Tennessee ? * Washington State ? * Alabama ? * Virginia ?? * Wisconsin ? * Illinois ? * Arizona ? * South Dakota ? * Montana ?? All of these opportunities are full-time and permanent. My clients offer outstanding compensation and benefits, including relocation and/or sign-on bonuses. {CONTACT.SALUTATION}, I also have incredible technical and leadership opportunities COMIN? IN HOT?daily! ? ? My opportunities are with the best employers in histology! ? I don?t work with big box anonymous labs; you can find those positions elsewhere. ? RELIA?s positions are permanent with small to medium-sized labs that offer growth and opportunity. ? I also collaborate with histology vendors and some research organizations. ? If you or any of your friends are interested or even curious about a new opportunity, Contact Me! ? ?? Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 You might never see these amazing opportunities unless you read my emails because most are RELIA Exclusives! ? Have a fantastic day! ? Thanks, Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker ?Pam Barker President/Senior Recruiting Specialist-Histology RELIA? Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From relia1 at earthlink.net Wed Aug 28 11:51:55 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 28 Aug 2024 12:51:55 -0400 Subject: [Histonet] Navigating the Histology Hiring Landscape: Challenges and Solutions. New Blog Post UP! Message-ID: <000401daf96a$981f03f0$c85d0bd0$@earthlink.net> Hi Histonetters, As we approach the latter part of 2024 and look ahead to the first quarter of 2025, the hiring landscape for histotechnologists remains challenging. The ongoing critical shortage of histotechs makes it more important than ever to plan your hiring strategies well in advance. ? As a matter of fact, I just wrote an article on this subject! ? Here are the links: LinkedIn: https://shorturl.at/Uh6IQ WordPress: Navigating the Histology Hiring Landscape: Challenges and Solutions | Histopeep Huddle (wordpress.com) Here is what I can do to HELP! ? At RELIA Solutions, I specialize in the nationwide permanent placement of histology professionals, and I?m here to help you navigate these challenges effectively. Here?s why planning ahead is crucial: * Stay Ahead of the Competition: Secure top candidates before they are snapped up by other employers. ? * Ensure Continuity of Services: Maintain high standards of patient care by avoiding staffing gaps. ? * Leverage Expertise: Benefit from my deep understanding of the histology job market and extensive network. ? * Customized Recruitment Strategies: Tailored solutions to meet your specific needs. ? Histonetters, here is a list of locations where some of my best candidates are now or want to move to! ? * Colorado * Florida * Texas * North Carolina * South Carolina * Georgia * Maryland * Washington DC * California * Tennessee * Washington State These are the techs I have talked to just THIS WEEK! * I chat with techs daily nationwide. * I know what they want, where they want to be, and when! ?? Let me help you navigate the complexities of the histology job market and find the best candidates for your lab. Contact Me! ? ? ? Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 Best regards, Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ???? From Kelly.Pairan at ohiohealth.com Thu Aug 29 14:03:31 2024 From: Kelly.Pairan at ohiohealth.com (Pairan, Kelly) Date: Thu, 29 Aug 2024 19:03:31 +0000 Subject: [Histonet] Issue with PAS Fungus in Nails Message-ID: Good afternoon, We are currently having an issue where our PAS Fungus in our nail specimens are too light more often than not. The control tissue (which is not nail) is staining just fine. We have not switched the time in sodium hydroxide (no more than 30 minutes) or the concentration (20%). A subsequent GMS shows organism so we know they should be positive. The organisms that are within the nail are pale but the ones outside the organism are fine. We have tested out two different kits with the same results. Does anyone have any suggestions of how to troubleshoot this? We are using a commercially prepared kit and they were received in June. The Schiff is kept in the fridge per the directions. All other kit components are kept at room temperature. Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: kelly.pairan at ohiohealth.com Work: (614) 566-3575 Cell: (614) 312-0104 From jmacdonald at mtsac.edu Thu Aug 29 14:10:19 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 29 Aug 2024 19:10:19 +0000 Subject: [Histonet] Issue with PAS Fungus in Nails In-Reply-To: References: Message-ID: Sodium hydroxide is a strong oxidizing agent. It might be oxidizing the chitin beyond the aldehyde stage. Get Outlook for iOS ________________________________ From: Pairan, Kelly via Histonet Sent: Thursday, August 29, 2024 12:03:31 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Issue with PAS Fungus in Nails EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Good afternoon, We are currently having an issue where our PAS Fungus in our nail specimens are too light more often than not. The control tissue (which is not nail) is staining just fine. We have not switched the time in sodium hydroxide (no more than 30 minutes) or the concentration (20%). A subsequent GMS shows organism so we know they should be positive. The organisms that are within the nail are pale but the ones outside the organism are fine. We have tested out two different kits with the same results. Does anyone have any suggestions of how to troubleshoot this? We are using a commercially prepared kit and they were received in June. The Schiff is kept in the fridge per the directions. All other kit components are kept at room temperature. Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: kelly.pairan at ohiohealth.com Work: (614) 566-3575 Cell: (614) 312-0104 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7Cjmacdonald%40mtsac.edu%7Cff70c28e966b44b95d8b08dcc85d59f9%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638605550497172577%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=jMQwfFh66Brtcit%2Fy%2BDTjY%2BMkJHePJBzK4VrEZ5i8CM%3D&reserved=0 From criley at udel.edu Fri Aug 30 09:57:18 2024 From: criley at udel.edu (Charles Riley) Date: Fri, 30 Aug 2024 10:57:18 -0400 Subject: [Histonet] Antibody dilution help Message-ID: Hello everyone, Not sure if it's just burnout or Covid brain but I am having issues with remembering the right way to dilute my antibodies, and all the calculators online seem to give different responses. I have 100 microliters of 1mg/mL antibody and I want to make a 1:100 dilution. How much diluent do I add to do this? And if anyone has any calculators that they like using that would be awesome too From patpxs at gmail.com Fri Aug 30 10:12:34 2024 From: patpxs at gmail.com (Paula Sicurello) Date: Fri, 30 Aug 2024 15:12:34 +0000 (UTC) Subject: [Histonet] Antibody dilution help In-Reply-To: References: Message-ID: <1917162660.1551561.1725030754199@mail.yahoo.com> Charles, The easiet way to remember it is this:? you want a 1:100 dilution - so you want 1 part antibody in 100 parts of diluent.? I was taught that meant add 1 part antibody to 100 parts of diluent.??However, there are some who add 1 part anitbody to 99 parts diluent.Thus you end up with 1 part antibody in 100 parts total. Some folks read 1:100 as 1 part in 100, some folks read it as add 1 part to 99 to make 100 parts. confused yet? ?I found this Let's see what others have to say. Sincerely, Paula Sicurello On Friday, August 30, 2024 at 07:57:36 AM PDT, Charles Riley via Histonet wrote: Hello everyone, Not sure if it's just burnout or Covid brain but I am having issues with remembering the right way to dilute my antibodies, and all the calculators online seem to give different responses. I have 100 microliters of 1mg/mL antibody and I want to make a 1:100 dilution. How much diluent do I add to do this?? And if anyone has any calculators that they like using that would be awesome too _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Fri Aug 30 11:19:07 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 30 Aug 2024 12:19:07 -0400 Subject: [Histonet] Happy Labor Day Histonetters!! And ICYMI a recap from earlier this week. Message-ID: <002b01dafaf8$5821bb50$086531f0$@earthlink.net> ? Happy Labor Day Weekend! ? As we celebrate the hard work and dedication of all professionals, I want to give a special shoutout to YOU! My amazing network of histology experts. Your commitment to excellence is truly inspiring! ?? ICYMI? As you enjoy the last of the Summer it may be the perfect time for me to Tune Up Your Resume! Resumes Are Not Just For Job Hunting. ? Going For A Promotion? ? Giving A Presentation ? Volunteeering ? Or A Great Way For You To See How Much You Have Accomplished. Shoot me an email to relia1 at earthlink.net ?with a copy of your resume. (preferably in Word) and I will take a look at it. If you are curious about new opportunities or actively looking. Either way let me know so I can be on the lookout >>?(BOLO) I am working with some amazing labs ? ? These clients rarely have openings because they are such ? GREAT places to WORK! ? They offer excellent compensation, benefits, and opportunities to grow, learn, and advance! Maybe you want to: ? Run Your Own Lab? ? Work in Vendor Support? ? Specialize in areas like IHC or Mohs? ? Take on a Leadership Role as a Lead Tech, Supervisor, or Manager? ? I have fantastic roles for leadership and growth, including: ? Directors ? Managers ? Supervisors ? Lead Techs ? IHC Specialist Most importantly, my clients are looking for Histotechs ready to learn, lead, grow, and prosper in: ? Immunohistochemistry?? ? Mohs?? ? Dermatopathology?? ? GI?? ? CancerDx?? ? Vendor Support ?? We have opportunities nationwide, including in: ? Florida?? ? Tennessee ? ? Washington State ? ? Alabama?? ? Virginia ?? ? Wisconsin?? ? Illinois?? ? Arizona?? ? South Dakota ? ? Montana??? All of these opportunities are full-time and permanent. My clients offer outstanding compensation and benefits, including relocation and/or sign-on bonuses. {CONTACT.SALUTATION}, I also have incredible technical and leadership opportunities COMIN? IN HOT?daily! ? ? My opportunities are with the best employers in histology! ? I don?t work with big box anonymous labs; you can find those positions elsewhere. ? RELIA?s positions are permanent with small to medium-sized labs that offer growth and opportunity. ? I also collaborate with histology vendors and some research organizations. ? If you or any of your friends are interested or even curious about a new opportunity, Contact Me! ???? Email: relia1 at earthlink.net Toll Free: 866-607-3542 Cell/Text: 407-353-5070 You might never see these amazing opportunities unless you read my emails because most are RELIA Exclusives!?? Have a fantastic Labor Day Weekend! ? Thanks, Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ????