From Jeffrey.Rinker at SanfordHealth.org Mon Apr 1 07:57:35 2024 From: Jeffrey.Rinker at SanfordHealth.org (Rinker,Jeff) Date: Mon, 1 Apr 2024 12:57:35 +0000 Subject: [Histonet] [EXTERNAL] Re: [EXT] Reagent alcohols and tissue processors In-Reply-To: References: <472887967.2172123.1711631073040.ref@mail.yahoo.com> <472887967.2172123.1711631073040@mail.yahoo.com> <544715091.2194403.1711635290080@mail.yahoo.com> Message-ID: First, I would like to say the Paloris is the best processor out there. This answers the second question too, I think. The standard that I have always used is 70% but with machines coming out now that are so good at monitoring the concertation's of the alcohols it is interesting to watch. ________________________________ From: Colleen Forster via Histonet Sent: Thursday, March 28, 2024 11:55 AM To: Paula Sicurello Cc: HistoNet Subject: [EXTERNAL] Re: [Histonet] [EXT] Reagent alcohols and tissue processors Paula, I start my processor at 80%. I am in research and often the samples have been fixed and are put into 70-80% ethanol for holding or transport. Starting with 80% steps right in line with the samples. I rarely see a schedule that starts at 50%. However, I do have a couple labs that ask me for that. I am fortunate to have a second processor I can use for special programs and not hold up my main runs. Colleen Forster HT(ASCP)QIHC University of Minnesota BLS Histology and IHC Laboratory On Thu, Mar 28, 2024 at 9:15?AM Paula Sicurello via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Kara, > I do like the VIPs. Besides getting rid of the NBF, the alcohols are > getting rid of the water as well. > I learned EM first, so my processing thoughts are probably biased by that. > Paula > > Yahoo Mail: Search, Organize, Conquer > > On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip > wrote: So for your first one I love the Sakura Tissue-Tek VIP. Easy to > use, easy maintenance, and very customizable for programed runs.Every lab I > have been in has always started at 70. It saves the techs extra time mixing > up dilutions because you can always find RTU 70%. It also helps I have > never had any issues with the tissues starting at 70. I get the idea of > starting lower but you are also then going to need to increase your > processing time which can delay turnaround times.Plus correct me if I am > wrong but isn't the whole point of the alcohols after NBF to get the NBF > out of the tissue and ready for xylene and wax? > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w:http://www.unthsc.edu/corelabs > > From: Paula Sicurello via Histonet > Sent: Thursday, March 28, 2024 8:04 AM > To: HistoNet > Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good > Morning, > My question two part: > Best tissue processor ever and why? > What is percentage is your first alcohol step? > I see that lots of places start at 70% but I favor a lower %. One > because it's gentler on the tissue, and two because 10% NBF precipitates > out if you start at 70%. > Thanks for your insight, > Paula > > Yahoo Mail: Search, Organize, Conquer > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From carl.hobbs at kcl.ac.uk Mon Apr 1 12:23:36 2024 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Mon, 1 Apr 2024 17:23:36 +0000 Subject: [Histonet] [EXT] Reagent alcohols and tissue Message-ID: I ask Researchers to give me cassettes in 90% alcohol as I start my "dipN dunk" processor ( Leica TP 1020) at 90% They fix, rinse in tap water, dist water, x2 70% alcohol, then bring to me in 90% alcohol This way, less likely to contaminate my 90% alc with their inadequate alcohol rinses. Been doin it for 30 yrs....OK so far ? Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From Phillip.Kara at unthsc.edu Tue Apr 2 08:46:50 2024 From: Phillip.Kara at unthsc.edu (Kara, Phillip) Date: Tue, 2 Apr 2024 13:46:50 +0000 Subject: [Histonet] IHC Validation Question Message-ID: Morning Histo Friends. Got a quick question for you. I am getting ready to go over to a new start up lab that will be doing all IHC staining in house. I have set up a lab before and done validations but that was on equipment and special stains which I will be doing again and feel pretty good about it. Any tips and tricks for validating IHCs? We will be using the Benchmark Ultra (please no solicitation we are all set). We are hoping not to have to buy too many controls so I have been looking to try finding some in house I can use to help save the cost. If anyone has any good advice for this to I will be all ears. Thanks in advance. Phillip Kara Senior Research Associate UNT HSC From Jessica.Piche at wtbyhosp.org Tue Apr 2 11:12:02 2024 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 2 Apr 2024 16:12:02 +0000 Subject: [Histonet] Premade Eosin Message-ID: Good Afternoon, I hope everyone is having a great week so far! Can anyone share what pre-made eosin brands they are using and recommend? We use Eosin Y. We make it ourselves and want to look into pre-made. Thank you in advance. Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From relia1 at earthlink.net Tue Apr 2 11:27:15 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 2 Apr 2024 12:27:15 -0400 Subject: [Histonet] New Blog Post UP! Essential Tips for Working With Recruiters in 2024! Message-ID: <00bf01da851a$a111e780$e335b680$@earthlink.net> Hi Histpeeps! It's Tip Tuesday! And I would like to invite you to check out my new blog post: Essential Tips for Working With A Recruiter in 2024! https://reliasolutionspambarker.wordpress.com/ Would love to hear what you think of it! I have exciting PERMANENT Positions NATIONWIDE! My clients are the CREAM of the CROP! They are paying the same or more than TRAVEL! Sign ON Bonuses up to 20K!! Just contact me - DM, email:relia1 at earthlink.net cell/text 407-353-5070 Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From bcooper at chla.usc.edu Tue Apr 2 11:44:17 2024 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Tue, 2 Apr 2024 16:44:17 +0000 Subject: [Histonet] Premade Eosin In-Reply-To: References: Message-ID: <20ceb226fb214093890b4e8ca3419262@chla.usc.edu> When we're not using our Ventana HE600 (their proprietary products), we use Eosin Y 515 from Leica. Our frozen section room uses Protocol Eosin-Y from Fisher. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Piche, Jessica via Histonet Sent: Tuesday, April 2, 2024 9:12 AM To: Histonet Subject: [Histonet] Premade Eosin (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good Afternoon, I hope everyone is having a great week so far! Can anyone share what pre-made eosin brands they are using and recommend? We use Eosin Y. We make it ourselves and want to look into pre-made. Thank you in advance. Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1euGCCUcb2FQpOeZDRnbuwbsEui_NYo_Xrwyzo6j5p-Xqd4P056Cv0WXWjzU-rUCUMsNEnU_HA3aQhuPRHdOqW8yIusd-ru6jqYbGYakiBQI04j5qnqTvCvGcxl0nQoJk3MbABBy5grgp8wcJvCErIdO5p0NliUt6DY47VFNw7hTvj0_nm6Sy3lEsZjZYwl0Br2GX2LuSL8jgGg3kw9hsJjAVnb_zbQAxpj2o09BU7c9s7PK7KOcXKqmLfDa33XzuHWWLScx_k4z3tDzI2DEnWZwdE9wOR0WJBVtzvNuwzY3qJzQMBng9sObf6z7PM2fmgVnx8oGzZpU5jZuSkvkF7uAnWgiCuuB0iKwOQ_jlVRVjbhtJ8T9INuewn2gL9Byqvz6Zl_kRXsCNLLk9NDPRlNNKv8MoUwVNL77Fn6h70wYWJwfpt3mJHDxYgF5G8LRs/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From greg.dobbin at gmail.com Tue Apr 2 12:17:24 2024 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Tue, 2 Apr 2024 14:17:24 -0300 Subject: [Histonet] IHC Validation Question Message-ID: Hi Kara, Use multi-tissue block controls with normal tissues that are treated exactly the same (pre-analytically speaking) as your routine surgical specimens. Consult NordiQC.org for recommended normal control tissue for each IHC marker. The most used multi-tissue control block in my lab has pieces of tonsil, pancreas, small bowel and liver. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 From relia1 at earthlink.net Wed Apr 3 10:25:57 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 3 Apr 2024 11:25:57 -0400 Subject: [Histonet] Getaway and Learn and Play in Orlando, FL Here is the info for the FSH Spring Meeting! Message-ID: <019501da85db$3ac87a40$b0596ec0$@earthlink.net> Hi Histonetters! I hope you are having a great day! How would you like to Getaway and Learn and Play in Orlando, FL? The Florida Society of Histotechnology Spring Meeting is May 3rd and 4th in Orlando!! * Connect with your fellow histopeeps! * Learn and earn CEUs! * Have a Great Time with Lunches and door prizes and A Happy HOUR! Want more info? Visit the FSH website to register: www.fshgroup.org * For schedule and more details check their social media on Facebook and Instagram! * For questions contact: Andrea Gualtieri at andreaghtl at gmail.com I will be doing a roundtable on Saturday "Ask a Recruiter"! I would love to see you there! I would love to have you join my Facebook Group. It's a group for histotechs to connect, ask questions and have fun. There are even some cool etsy people with some amazing histology swag in my group! Here is the link: www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From Jennifer.Wooten at tricore.org Wed Apr 3 15:37:41 2024 From: Jennifer.Wooten at tricore.org (Wooten, Jennifer) Date: Wed, 3 Apr 2024 20:37:41 +0000 Subject: [Histonet] RFID Tracking Message-ID: Good afternoon, Does anyone use RFID to track specimens in their laboratories? Thank you, Jennifer Wooten, BA, BS, HTL (ASCP)CM ......................................................................................................................... STATEMENT OF CONFIDENTIALITY:This message originates from TriCore, Rhodes Group, or an affiliate organization or representative. Please note that the information contained in this message, including any attachments, is confidential and intended for the use of the named recipient. If the reader of this message is any other than the individual or entity named above or an agent responsible for delivery, you are hereby notified that any inappropriate dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately. Any errant copy of this message must be deleted or destroyed. NOTE: The confidentiality of the information disclosed to you may be protected by law, including Section 24-2B-7 NMSA. State law prohibits you from making further disclosure of such information without specific written consent of the person to whom the information pertains or as otherwise permitted by state law. From relia1 at earthlink.net Fri Apr 5 11:18:26 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 5 Apr 2024 12:18:26 -0400 Subject: [Histonet] FIRST Look! RELIA HOT Job Alert! Exciting Opportunity North of Orlando - A RELIA Exclusive!! Message-ID: <014b01da8774$e48860f0$ad9922d0$@earthlink.net> Hi Histonetters! I hope you are having a great Friday! I have a BRAND New Exciting Opportunity that I want to share with you! I know there are histopeeps out there trying to get to Central Florida! Here's your chance! Exciting Opportunity North of Orlando! FIRST Look!! RELIA HOT JOB ALERT!!! A RELIA EXCLUSIVE!!! Full time PERM and 1st Shift!! Want more info? Contact me - Pam Barker email:relia1 at earthlink.net cell/text 407-353-5070 Have a Fantastic Weekend!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From albert.dotson at duke.edu Mon Apr 8 14:14:06 2024 From: albert.dotson at duke.edu (Bert Dotson) Date: Mon, 8 Apr 2024 19:14:06 +0000 Subject: [Histonet] RFID Tracking In-Reply-To: References: Message-ID: Hi Jennifer, We piloted RFID tracking of completed slides about 10 or more years ago and could not find any value in it. We are currently piloting RFID tracking of specimens from our offsite clinics. This seems to be more promising. Regards, Bert Bert Dotson, MBA, HTL (ASCP) Anatomic Pathology IT Analyst, DUHS Clinical Labs albert.dotson at duke.edu ? The information in this electronic mail is sensitive, protected information intended only for the addressee(s). Any other person, including anyone who believes he/she might have received it due to an addressing error, is requested to notify the sender immediately by return electronic mail, and to delete it without further reading or retention. The information is not to be forwarded or shared in any manner unless in compliance with the Duke University policies on confidentiality and/or with the approval of the sender. -----Original Message----- From: Wooten, Jennifer Sent: Wednesday, April 3, 2024 4:38 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] RFID Tracking Good afternoon, Does anyone use RFID to track specimens in their laboratories? Thank you, Jennifer Wooten, BA, BS, HTL (ASCP)CM ......................................................................................................................... STATEMENT OF CONFIDENTIALITY:This message originates from TriCore, Rhodes Group, or an affiliate organization or representative. Please note that the information contained in this message, including any attachments, is confidential and intended for the use of the named recipient. If the reader of this message is any other than the individual or entity named above or an agent responsible for delivery, you are hereby notified that any inappropriate dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately. Any errant copy of this message must be deleted or destroyed. NOTE: The confidentiality of the information disclosed to you may be protected by law, including Section 24-2B-7 NMSA. State law prohibits you from making further disclosure of such information without specific written consent of the person to whom the information pertains or as otherwise permitted by state law. From relia1 at earthlink.net Tue Apr 9 11:43:57 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 9 Apr 2024 12:43:57 -0400 Subject: [Histonet] ASCP and CLIA qualified histotech available for your area! Message-ID: <004301da8a9d$1efee210$5cfca630$@earthlink.net> Hello Histonetters , I hope you are doing well. I wanted to touch base because I know how difficult it is to find histotechs and I am working with another great tech that could be a great addition to your team. Here is her profile: * She is ASCP certified CLIA Qualified to Gross and has experience in hospital, research and independent lab settings. * She has also functioned in lead tech roles. * She is looking for a pay rate of about 30 per hour (cost of living considered). * She is willing to work any shift but prefers 1st or 2nd. I wanted to reach out to you because somebody is going to snap her up and I wanted to give you first shot. Let me know if you would like more information. Thanks for taking the time to read my email! Have a Great DAY! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From marielachertoff at gmail.com Wed Apr 10 09:28:17 2024 From: marielachertoff at gmail.com (Mariela Chertoff) Date: Wed, 10 Apr 2024 11:28:17 -0300 Subject: [Histonet] Golgi-cox staining Message-ID: Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar From jkiernan at uwo.ca Wed Apr 10 23:22:02 2024 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 11 Apr 2024 04:22:02 +0000 Subject: [Histonet] Golgi-cox staining In-Reply-To: References: Message-ID: I suggest asking Dr Sami Zaqout, the corresponding author of the paper you cited. A German email address is given on the left side of the first page of the free PDF file for which you provided a web link: https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/ful. He is easy to find with Google and he is now at Qatar University. It's the most thorough description of Golgi-Cox staining that I've seen, and the pictures of results look superb. Vibratome sectioning evidently is better and quicker than embedding in nitrocellulose and cutting with a sliding microtome! The two authors' use of the word "cryoprotectant" was unfortunate in the context of a technique that does not involve freezing. John Kiernan Emeritus, Anatomy, University of Western Ontario, Canada https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Mariela Chertoff via Histonet Sent: April 10, 2024 10:28 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Golgi-cox staining Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick at northwestern.edu Thu Apr 11 09:30:47 2024 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Thu, 11 Apr 2024 14:30:47 +0000 Subject: [Histonet] FW: Golgi-cox staining In-Reply-To: References: Message-ID: From: Rizaldy P Scott Sent: Wednesday, April 10, 2024 4:33 PM To: Bernice Frederick Subject: Re: [Histonet] Golgi-cox staining Hi Bernice, What they should have done ideally is to store the uncut agarose blocks submerged in whatever buffer they used to rinse the brains out prior to agarose embedding. They can probably salvage the dried-out brains by rehydrating in their buffer or 30% sucrose solution overnight (or store at 4 degrees as needed) and re-embed in agarose only when they are ready to cut. I can't guarantee however if artefacts due to drying might result but it's worth the shot. Best regards, Riz Rizaldy P. Scott, M.S., Ph.D. Research Associate Professor of Pathology Scientific Director Mouse Histology & Phenotyping Laboratory Robert H. Lurie Comprehensive Cancer Center Northwestern University Feinberg School of Medicine ?Olson Pavilion, Room 8-335, 710 N. Fairbanks Ct., Chicago, IL 60611 ?? rizaldy.scott at northwestern.edu ? +1-(312)-503-2695 https://www.feinberg.northwestern.edu/sites/mhpl/ www.cancer.northwestern.edu ________________________________ From: Bernice Frederick > Sent: Wednesday, April 10, 2024 13:40 To: Rizaldy P Scott > Subject: FW: [Histonet] Golgi-cox staining Can you help this person? Bernice -----Original Message----- From: Mariela Chertoff via Histonet > Sent: Wednesday, April 10, 2024 9:28 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Golgi-cox staining Hi all We made the Golgi cox staining and due to a problem with the vibratome, we left the tissue several days embedded in agarose and they get dryed, It is possoble to recover the brains? it is better to repeat the agarose embebbing o it is better to put the brains in crioprerervate solution to rehidrated and after that put them in agarosa again? We are following the Zaquot protocol https://urldefense.com/v3/__https://www.frontiersin.org/articles/10.3389/fnana.2016.00038/full__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848hoVaCzp$ Thanks in advance for your reply Mariela Chertoff, PhD Laboratorio de Neuroepigenetica - QB75 Departamento de Qu?mica Biol?gica Facultad de Ciencias Exactas y Naturales - UBA Ciudad Universitaria Pabell?n II Piso 4 Ciudad Aut?noma de Buenos Aires C1428EGA - Argentina Tel: 54 11 5285-8680/1/2 email:marielachertoff at gmail.com marielachertoff at qb.fcen.uba.ar > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!QkwV4_RJl5cyhElJgSA-8lRMmOSmkwVoHzRrMg8-ZXGX0yUzNCxM_pm09t45KaZWo04kpjD-tBduP59_0DHjxeEvlRMxvl848ty2UFsq$ From relia1 at earthlink.net Thu Apr 11 12:18:42 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 11 Apr 2024 13:18:42 -0400 Subject: [Histonet] ICYMI: YES! You Should Ask For A Raise Via Email and Here's HOW! Plus A "Plan B" Message-ID: <024101da8c34$4ea6c030$ebf44090$@earthlink.net> Hey Histonetters! I hope you are having a great day! I have been hearing from a lot of your fellow histopeeps asking for strategies on how to ask for a raise. I saw this great article - I could not have written it better myself LOL! So why reinvent the wheel? ICYMI: How to Ask for a Rais VIA Email - a Step by Step Guide! And a few Interesting Opportunities! https://www.themuse.com/advice/how-to-ask-for-a-raise-via-email If you are unsuccessful in your quest for a raise... OR Are Interested in a New Opportunity... Here Are The Exciting Opportunities that I am currently working on. Leadership and Specialty positions: IHC Tech CA - S.F. Bay Area Mohs Tech AZ - Tucson Histo Supervisor SD - Rapid City Histo Supervisor GA - Atlanta Here are the Tech Positions: HistoTech VA - Salem Histotech VA - Virginia Beach Histotech AL - Birmingham Histotech FL - Ft. Myers IHC Tech VA - Virginia Beach Mohs Tech AZ - Tucson Histotech TN - Chattanooga Histotech VA - Charlottesville You can Use your histology skills to contribute, grow and learn! My clients are in need of amazing histotechs Like YOU!! My clients are offering exciting opportunities that are permanent full-time positions with excellent compensation and benefits, and relocation/sign on bonuses up to 20K! You can FINISH your Travel Assignment! You can TAKE your vacation time! Would you be interested in more information on any these positions? OR Do you know of anyone who might be interested? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. If you or someone you know might be interested, please contact me. I can be reached: toll free at 866-607-3542 cell/text407-353-5070 email me at relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas, please let me know. I am getting calls for histology professionals at all levels practically everywhere on almost DAILY basis!! Join your fellow histopeeps for fun and information at my Facebook group just for you!! www.facebook.com/groups/histotechnologists I just need 25 more members to get to 5K!!! Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From dogsloveus2 at yahoo.com Fri Apr 12 15:06:49 2024 From: dogsloveus2 at yahoo.com (dogsloveus2) Date: Fri, 12 Apr 2024 20:06:49 +0000 (UTC) Subject: [Histonet] Histonet Digest, Vol 245, Issue 9 In-Reply-To: References: Message-ID: <1584658668.9445095.1712952409618@mail.yahoo.com> Hi Pam! Just curious about the position in Salem, Virginia.? Is it a permanent position?? Do you have details on it??? Thanks!? Tara Yahoo Mail: Search, Organize, Conquer On Fri, Apr 12, 2024 at 12:11 PM, histonet-request at lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to ??? histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit ??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to ??? histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at ??? histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: ? 1. ICYMI: YES! You Should Ask For A Raise Via Email and??? Here's ? ? ? HOW! Plus A "Plan B" (relia1 at earthlink.net) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 Apr 2024 13:18:42 -0400 From: To: Subject: [Histonet] ICYMI: YES! You Should Ask For A Raise Via Email ??? and??? Here's HOW! Plus A "Plan B" Message-ID: <024101da8c34$4ea6c030$ebf44090$@earthlink.net> Content-Type: text/plain;??? charset="us-ascii" Hey Histonetters! I hope you are having a great day! I have been hearing from a lot of your fellow histopeeps asking for strategies on how to ask for a raise. I saw this great article - I could not have written it better myself LOL! So why reinvent the wheel? ICYMI: How to Ask for a Rais VIA Email - a Step by Step Guide!? And a few Interesting Opportunities! https://www.themuse.com/advice/how-to-ask-for-a-raise-via-email If you are unsuccessful in your quest for a raise... OR Are Interested in a New Opportunity... Here Are The Exciting Opportunities that I am currently working on. Leadership and Specialty positions: IHC Tech? ? ? ? ? CA - S.F. Bay Area Mohs Tech? ? ? ? AZ - Tucson Histo Supervisor? SD - Rapid City Histo Supervisor? GA - Atlanta Here are the Tech Positions: HistoTech? ? ? ? VA - Salem Histotech? ? ? ? VA - Virginia Beach Histotech? ? ? ? AL - Birmingham Histotech? ? ? ? FL - Ft. Myers IHC Tech? ? ? ? ? VA - Virginia Beach Mohs Tech? ? ? ? AZ - Tucson Histotech? ? ? ? TN - Chattanooga Histotech? ? ? ? VA - Charlottesville You can Use your histology skills to contribute, grow and learn! My clients are in need of amazing histotechs Like YOU!! My clients are offering exciting opportunities that are permanent full-time positions with excellent compensation and benefits, and relocation/sign on bonuses up to 20K! You can FINISH your Travel Assignment! You can TAKE your vacation time! Would you be interested in more information on any these positions? OR Do you know of anyone who might be interested? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. If you or someone you know might be interested, please contact me. I can be reached: toll free at 866-607-3542 cell/text407-353-5070 email me at relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas, please let me know.? I am getting calls for histology professionals at all levels? practically everywhere on? almost DAILY basis!! Join your fellow histopeeps for fun and information at my Facebook group just for you!! www.facebook.com/groups/histotechnologists I just need 25 more members to get to 5K!!! Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? ? ? (407)353-5070 FAX:? ? ? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail:? relia1 at earthlink.net? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 245, Issue 9 **************************************** From relia1 at earthlink.net Mon Apr 15 11:31:43 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 15 Apr 2024 12:31:43 -0400 Subject: [Histonet] Happy Lab Week! ICYMI: Some Exciting and Exclusive Opportunities! Message-ID: <000901da8f52$67241880$356c4980$@earthlink.net> Hi Histopeeps! Happy Lab Week!! Be careful what you search for! If you search "Lab Week" you might get labrador retrievers instead of lab professionals. I know... It happened to me! That's why I couldn't resist this pic! I hope you have a GREAT day and A Fantastic WEEK with lots of appreciation, snacks and swag!! ICYMI: I have some amazing NEW and EXCLUSIVE Opportunities in >Arizona >California >Florida >Tennessee >Virginia >South Dakota >Georgia >Alabama MY clients are looking for: **Talented Histotechs** **Amazing IHC Techs** **Inspiring Leaders** Contact me for more info! email me relia1 at earthlink.net cell/text 407-353-5070 #labweek2024 #happylabweek #MondayMotivation #Monday #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Mon Apr 15 11:37:13 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 15 Apr 2024 12:37:13 -0400 Subject: [Histonet] Repost with pic as attachment? Happy Lab Week Histonetters!! and ICYMI: Some exciting NEW Job opportunities! Message-ID: <001801da8f53$2c00abf0$840203d0$@earthlink.net> Hi Histopeeps! Happy Lab Week!! Be careful what you search for! If you search "Lab Week" you might get labrador retrievers instead of lab professionals. I know... It happened to me! That's why I couldn't resist this pic! I hope you have a GREAT day and A Fantastic WEEK with lots of appreciation, snacks and swag!! ICYMI: I have some amazing NEW and EXCLUSIVE Opportunities in Arizona California Florida Tennessee Virginia South Dakota Georgia Alabama My clients are looking for Talented Histotechs Amazing IHC Techs Inspiring Leaders Contact me for more info! message me email me relia1 at earthlink.net cell/text 407-353-5070 #labweek2024 #happylabweek #MondayMotivation #Monday #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From mward at wakehealth.edu Tue Apr 16 08:42:40 2024 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Tue, 16 Apr 2024 13:42:40 +0000 Subject: [Histonet] How are you documenting precision in IHC validations Message-ID: We are working on IHC antibody validations and I want to know if there is a minimum number of slides you must run to demonstrate precision? I have not been able to find a definitive number or procedure. What is everyone doing for this? Thanks in advance for everyone's help. Martha Ward Atrium Health Wake Forest Baptist ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From relia1 at actsend.com Tue Apr 16 10:03:12 2024 From: relia1 at actsend.com (Pam Barker) Date: Tue, 16 Apr 2024 15:03:12 +0000 Subject: [Histonet] Here are your 2024 Lab Week Logos to use and Share! And some exciting New and Exclusive Opportunities!! Message-ID: <7D.B1.16559.0339E166@if.mta1vrest.cc.prd.sparkpost> Hi?Histopeeps ! Happy Lab Week! I hope you are enjoying lots of snacks and swag and most of all APPRECIATION! Because you deserve it. Here are 2 of the logos for Medical Laboratory Professionals Week for you to enjoy and share! I want to show you my appreciation by reminding you of all of things I can, want to and will do for YOU. Why?? Because I LOVE my histopeeps! Referral program: ???????? 250 for referring a candidate that I place with one of my clients ???????? 250 for referring a job opening that I place one of my candidates in. Complimentary Career Services: ???????? Need help with a resume? ???????? Coaching for an interview or promotion? ???????? Salary Negotiation or asking for a raise? ???????? Transitioning From travel to perm or vice versa? ???????? Information about goings on in the field of histology ???????? Looking for a NEW Opportunity? I can help with ALL of this for YOU! Histopeeps?! ?Here is a list of my current exciting opportunities. ALL of these are Permanent Positions. Most are RELIA Exclusives! My clients off as much or more than travel assignments with relo/sign ons up to 20K! Leadership and Specialty positions: ???????? Mohs Tech? ? ? ? ? ? ? ?AZ - Tucson ???????? Histo Supervisor? SD - Rapid City ???????? Histo Supervisor? GA - Atlanta Here are the Tech Positions: ???????? Histotech???????? VA - Virginia Beach ???????? Mohs Tech???????AZ - Tucson ???????? Histotech???????? AL - Birmingham ???????? Histotech???????? FL - Ft. Myers ???????? IHC Tech????????? VA - Virginia Beach ???????? Mohs Tech???????AZ - Tucson ???????? Histotech???????? TN - Chattanooga ???????? Histotech???????? VA - Charlottesville You can Use your histology skills to contribute, grow and learn! ? My clients are in need of amazing histotechs Like YOU!! ? My clients are offering exciting opportunities that are permanent full-time positions ? with excellent compensation and benefits, and relocation/sign on bonuses up to 20K! ? You can FINISH your Travel Assignment! ? You can TAKE your vacation time! Would you be interested in more information on any these positions? OR Do you know of anyone who might be interested? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. If you or someone you know might be interested, please contact me. I can be reached: ? toll free at 866-607-3542 ? cell/text407-353-5070 ? email me at relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas, please let me know.? I am getting calls for histology professionals at all levels? practically everywhere on? almost DAILY basis!! Have a Fantastic Day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community!? Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology RELIA Solutions? 5703 Red Bug Lake Road #330
Winter Springs, fl 32708 ? Click Here to Unsubscribe From mward at wakehealth.edu Tue Apr 16 12:36:38 2024 From: mward at wakehealth.edu (Martha Ward-Pathology) Date: Tue, 16 Apr 2024 17:36:38 +0000 Subject: [Histonet] Precision in IHC validations Message-ID: Thanks to everyone that has responded to my question. We specifically interested in the procedure for this CAP question: COM.40350 Validation of Test Performance Specifications - Modified FDA-cleared/ approved Tests and LDTs Prior to clinical use of each modified FDA-cleared or approved test and laboratory developed tests (LDTs), the laboratory has performed a validation study and prepared a written assessment of each of the following test method performance specifications, as applicable, using a sufficient number of characterized samples: ? Analytical accuracy ? Analytical precision ? Reportable range ? Analytical sensitivity (lower detection limit) ? Analytical specificity ? Any other performance characteristic required to ensure analytical test performance Do you comment at all regarding precision?..do you run the same slide each day over 3 days, or something like that? NOTE 3: Precision is validated by repeat measurement of samples at varying concentrations or activities within-run and between-run over a period of time. We are just not sure what number of repeats is best?.. ________________________________ This electronic message is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From relia1 at earthlink.net Wed Apr 17 09:33:23 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 17 Apr 2024 10:33:23 -0400 Subject: [Histonet] My apologies re: Lab Week Logos Message-ID: <013001da90d4$3db7e7a0$b927b6e0$@earthlink.net> Hi Histonetters, I posted yesterday about Lab Week Logos. I realize that I can't post images on histonet. If you want the logos just shoot me an email. I have the official ASCP logo for lab week and Also another one that says Peace Love Lab. Let me know if you want the images Happy Lab Week! Have a Great Day! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From jmacdonald at mtsac.edu Fri Apr 19 01:28:18 2024 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Fri, 19 Apr 2024 06:28:18 +0000 Subject: [Histonet] California Society for Histotechnology Symposium Message-ID: Our California Society for Histotechnology Symposium is coming up. This is a great way to earn those CEs and network with fellow histotechs. Please share this information with your colleagues. You will find more information on our website. The deadline for hotel reservations is April 23. Thank you, Jennifer MacDonald CSH Education Chair From wcavett at umc.edu Sat Apr 20 18:14:41 2024 From: wcavett at umc.edu (Will Cavett) Date: Sat, 20 Apr 2024 23:14:41 +0000 Subject: [Histonet] CYP.04510 Message-ID: Hello. What are labs doing to be compliant with this CAP regulation? Any ideas and suggestions would be greatly appreciated. Get Outlook for iOS Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From relia1 at earthlink.net Mon Apr 22 12:21:56 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 22 Apr 2024 13:21:56 -0400 Subject: [Histonet] It's Motivation Monday and there's still time to sign up and attend your state's histology meetings! Message-ID: <004501da94d9$93cd4b90$bb67e2b0$@earthlink.net> Hi Histonetters! State Histo Meetings!!!! There is still time to participate! Check your state society and nearby state society websites for info! Also some are even offering a VIRTUAL option! That's my Monday Motivation to remind you of what a fantastic way to network and learn with your fellow histotechs! If you can't find info on nearby or virtual meetings contact me and I will get the info for you! Why? Because I LOVE MY HISTOPEEPS! Have a GREAT Day! #Monday #MondayMotivation #Ilovemyhistopeeps #histologyiscool #histologylab #histology #jobsformyhistopeeps #histologyjobs #histologycareers Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! From relia1 at earthlink.net Wed Apr 24 12:03:37 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 24 Apr 2024 13:03:37 -0400 Subject: [Histonet] Ya Know That ONE Place YOU always wanted to LIVE? Is it on this LIST? Message-ID: <000901da9669$5a478520$0ed68f60$@earthlink.net> Hi Histonetters! Happy Wednesday! Yeah it's hump day but Wednesday is my favorite day of the week because I get most of my greatest opportunities! I am seeing a trend. I am hearing from clients in places that have VERY LOW TURNOVER! I KNOW there are histopeeps out there JUST WAITING for the RIGHT Opportunity in The PERFECT LOCATION! I am here to tell you it can happen anytime! Here are some examples of the areas I am working on RIGHT NOW! Montana Arizona South Dakota Oregon Indiana Virginia Tennesee Alabama Georgia Florida And new opportunities coming in all of the time! Is there a location I should add to my list? These are permanent full time positions and my clients offer excellent compensation, relo/sign on bonuses up to 20K and a fantastic team to work with! If you would like more info... Contact ME! email: relia1 at earthlink.net cell/text 407-353-5070 Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From thisisann at aol.com Wed Apr 24 13:59:37 2024 From: thisisann at aol.com (Ann Specian) Date: Wed, 24 Apr 2024 18:59:37 +0000 (UTC) Subject: [Histonet] Epredia xylene substitute References: <1779091641.2783754.1713985177046.ref@mail.yahoo.com> Message-ID: <1779091641.2783754.1713985177046@mail.yahoo.com> We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene?substitute that they could share? Sent from the all new AOL app for iOS From john at imebinc.com Thu Apr 25 12:13:37 2024 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Thu, 25 Apr 2024 10:13:37 -0700 Subject: [Histonet] Histonet Digest, Vol 245, Issue 17 In-Reply-To: References: Message-ID: <8FAB6CDA-A571-4889-8E1F-87287F5D9FD5@imebinc.com> Very slow drying of all substitutes > On Apr 25, 2024, at 10:11?AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ya Know That ONE Place YOU always wanted to LIVE? Is it on > this LIST? (relia1 at earthlink.net) > 2. Epredia xylene substitute (Ann Specian) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 24 Apr 2024 13:03:37 -0400 > From: > To: > Subject: [Histonet] Ya Know That ONE Place YOU always wanted to LIVE? > Is it on this LIST? > Message-ID: <000901da9669$5a478520$0ed68f60$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters! > > Happy Wednesday! Yeah it's hump day but > > Wednesday is my favorite day of the week because I get most of > > my greatest opportunities! > > I am seeing a trend. > > I am hearing from clients in places that have VERY LOW TURNOVER! > > I KNOW there are histopeeps out there JUST WAITING for the RIGHT Opportunity > in > > The PERFECT LOCATION! > > I am here to tell you it can happen anytime! > > Here are some examples of the areas I am working on > > RIGHT NOW! > > Montana > > Arizona > > South Dakota > > Oregon > > Indiana > > Virginia > > Tennesee > > Alabama > > Georgia > > Florida > > And new opportunities coming in all of the time! > > Is there a location I should add to my list? > > These are permanent full time positions and my clients offer excellent > compensation, relo/sign on bonuses up to 20K > > and a fantastic team to work with! > > If you would like more info... > > Contact ME! > > email: relia1 at earthlink.net > > cell/text 407-353-5070 > > > > Thanks-Pam > > > > Right Time, Right Place, Right Move with RELIA! > > Providing excellent service exclusively to the Histology Community! > > > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5717 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > > Toll free: (866)60RELIA or (866)607-3542 > E-mail: relia1 at earthlink.net > > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > > > www.linkedin.com/in/reliasolutions > > #ilovemyhistopeeps > > #jobs4myhistopeeps > > #histologyiscool > > #histologyjobs > > #histologycareers > > #histology > > > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 24 Apr 2024 18:59:37 +0000 (UTC) > From: Ann Specian > To: > Subject: [Histonet] Epredia xylene substitute > Message-ID: <1779091641.2783754.1713985177046 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene?substitute that they could share? > > > Sent from the all new AOL app for iOS > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 245, Issue 17 > ***************************************** From Karen.Schieberl at commonspirit.org Thu Apr 25 12:43:26 2024 From: Karen.Schieberl at commonspirit.org (Karen Schieberl CA-San Francisco) Date: Thu, 25 Apr 2024 10:43:26 -0700 Subject: [Histonet] Premaking slides Message-ID: Good Morning, I am not sure if there is a CAP or Joint commission Policy on pre making slides for intraoperative consultation. Can someone let me know if there is one? I have looked but was unable to find one. It just seems like errors can be made especially if you have multiple patients or for some reason the information on the slide does not match the patient. Any help would be greatly appreciated. Thanks, Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From rsrichmond at gmail.com Thu Apr 25 14:52:35 2024 From: rsrichmond at gmail.com (Bob Richmond) Date: Thu, 25 Apr 2024 15:52:35 -0400 Subject: [Histonet] Histonet Digest, Vol 245, Issue 17 In-Reply-To: References: Message-ID: Ann Specian (where?) asks about Epredia Xylene Substitute: >>We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene?substitute that they could share?<< Well, what?s in it? You?re asking your readers to look it up for you. I Googled it, and found the SDS (materials safety data sheet) buried many pages down in the Epredia web site: https://epredia.webdamdb.com/bp/#/folder/7477836/104813679 It?s identified only as ?naphtha?. That?s a petroleum cut that?s heavier than gasoline, more like kerosene. Dry-cleaners used it before they went to less flammable alternatives. So it?s an ?aliphatic? clearing agent, but a very crude one. If you want an aliphatic (rather than a turpentine-like lemon-smelling preparation), I?d find one specially formulated for tissue processing, rather than this repurposed cleaning fluid. Bob Richmond Maryville, TN On Thu, Apr 25, 2024 at 1:14?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Ya Know That ONE Place YOU always wanted to LIVE? Is it on > this LIST? (relia1 at earthlink.net) > 2. Epredia xylene substitute (Ann Specian) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 24 Apr 2024 13:03:37 -0400 > From: > To: > Subject: [Histonet] Ya Know That ONE Place YOU always wanted to LIVE? > Is it on this LIST? > Message-ID: <000901da9669$5a478520$0ed68f60$@earthlink.net> > Content-Type: text/plain; charset="us-ascii" > > Hi Histonetters! > > Happy Wednesday! Yeah it's hump day but > > Wednesday is my favorite day of the week because I get most of > > my greatest opportunities! > > I am seeing a trend. > > I am hearing from clients in places that have VERY LOW TURNOVER! > > I KNOW there are histopeeps out there JUST WAITING for the RIGHT > Opportunity > in > > The PERFECT LOCATION! > > I am here to tell you it can happen anytime! > > Here are some examples of the areas I am working on > > RIGHT NOW! > > Montana > > Arizona > > South Dakota > > Oregon > > Indiana > > Virginia > > Tennesee > > Alabama > > Georgia > > Florida > > And new opportunities coming in all of the time! > > Is there a location I should add to my list? > > These are permanent full time positions and my clients offer excellent > compensation, relo/sign on bonuses up to 20K > > and a fantastic team to work with! > > If you would like more info... > > Contact ME! > > email: relia1 at earthlink.net > > cell/text 407-353-5070 > > > > Thanks-Pam > > > > Right Time, Right Place, Right Move with RELIA! > > Providing excellent service exclusively to the Histology Community! > > > > Thank You! > Pam M. Barker > > Pam Barker > President/Senior Recruiting Specialist-Histology > RELIA Solutions > Specialists in Allied Healthcare Recruiting > 5717 Red Bug Lake Road #330 > Winter Springs, FL 32708-4969 > Phone: (407)657-2027 > Cell: (407)353-5070 > FAX: (407)678-2788 > > Toll free: (866)60RELIA or (866)607-3542 > E-mail: relia1 at earthlink.net > > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > > > www.linkedin.com/in/reliasolutions > > #ilovemyhistopeeps > > #jobs4myhistopeeps > > #histologyiscool > > #histologyjobs > > #histologycareers > > #histology > > > > > > > > ------------------------------ > > Message: 2 > Date: Wed, 24 Apr 2024 18:59:37 +0000 (UTC) > From: Ann Specian > To: > Subject: [Histonet] Epredia xylene substitute > Message-ID: <1779091641.2783754.1713985177046 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > We are thinking of changing from xylene to this substitute. Does anybody > have any Processing protocols using Epredia Xylene?substitute that they > could share? > > > Sent from the all new AOL app for iOS > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 245, Issue 17 > ***************************************** > From jaylundgren at gmail.com Thu Apr 25 15:19:51 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 25 Apr 2024 15:19:51 -0500 Subject: [Histonet] Premaking slides In-Reply-To: References: Message-ID: You mean for frozens? On Thu, Apr 25, 2024 at 12:44?PM Karen Schieberl CA-San Francisco via Histonet wrote: > Good Morning, > I am not sure if there is a CAP or Joint commission Policy on pre making > slides for intraoperative consultation. Can someone let me know if there > is one? I have looked but was unable to find one. It just seems like > errors can be made especially if you have multiple patients or for some > reason the information on the slide does not match the patient. Any help > would be greatly appreciated. > > Thanks, > Karen Schieberl HT ASCP > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.schieberl@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karen.Schieberl at commonspirit.org Thu Apr 25 15:30:42 2024 From: Karen.Schieberl at commonspirit.org (Karen Schieberl CA-San Francisco) Date: Thu, 25 Apr 2024 13:30:42 -0700 Subject: [Histonet] Premaking slides In-Reply-To: References: Message-ID: This does apply to Frozen and Touch preps. Karen Schieberl HT ASCP Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.schieberl@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Thu, Apr 25, 2024 at 1:20?PM Jay Lundgren wrote: > *USE CAUTION - EXTERNAL EMAIL* > ------------------------------ > You mean for frozens? > > On Thu, Apr 25, 2024 at 12:44?PM Karen Schieberl CA-San Francisco via > Histonet wrote: > >> Good Morning, >> I am not sure if there is a CAP or Joint commission Policy on pre making >> slides for intraoperative consultation. Can someone let me know if there >> is one? I have looked but was unable to find one. It just seems like >> errors can be made especially if you have multiple patients or for some >> reason the information on the slide does not match the patient. Any help >> would be greatly appreciated. >> >> Thanks, >> Karen Schieberl HT ASCP >> >> Lead Histology Technician >> >> St. Mary's Medical Center >> >> 450 Stanyan St. >> >> San Francisco, Ca. 94117 >> >> 415-750-5751 >> >> karen.schieberl@ commonspirit.org >> >> >> >> >> Caution: This email message, including all content and attachments, is >> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The >> information contained in this email message is intended only for the use >> of >> the recipient(s) named above. If the reader of this message is not the >> intended recipient or an agent responsible for delivering it to the >> intended recipient, you have received this document in error. Any further >> review, dissemination, distribution, or copying of this message is >> strictly >> prohibited. If you have received this communication in error, please >> notify us immediately by reply email. Thank you >> >> Caution: This email is both proprietary and confidential, and not >> intended for transmission to (or receipt by) any unauthorized person(s). If >> you believe that you have received this email in error, do not read any >> attachments. Instead, kindly reply to the sender stating that you have >> received the message in error. Then destroy it and any attachments. Thank >> you. >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > From jaylundgren at gmail.com Thu Apr 25 16:16:14 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 25 Apr 2024 16:16:14 -0500 Subject: [Histonet] Premaking slides In-Reply-To: References: Message-ID: I mean, you're not going to hand the pathologist an unlabelled slide, obviously. This leaves you two options, label the slide before the tissue is on it, or afterward. Labelling the slide before is easier and quicker. I'm gonna label my slides *immediately* before cutting the frozen, or squishing the tissue. Patient name at the very least, if it hasn't been accessioned yet. But you don't want to leave any labeled blank slides laying around, that's asking for trouble. If you label too many slides, for instance, discard them immediately. If you are doing multiple cases at once, you are doing it wrong. I know a lot of places operate like this, but frozens are supposed to be scheduled for a reason. I mean, you might get an occasional emergency frozen for an ectopic pregnancy, but that's rare. There is a scheduler in surgery, they should be doing their job. Or maybe the head of Pathology needs to talk to the head of Surgery and explain how risky it is to be dealing with multiple frozens at once. Especially if it's the same tissue type. I'll probably get a lot of flak for this viewpoint, "Oh that's impossible at my institution!". But it's not. I've dealt with this as manager at several institutions and it can be fixed. It's not going to make you any friends in Surgery, but it's ultimately about patient safety. It's just that most hospitals are terrified of surgeons and will let them do whatever they want. How about the surgeon that leaves the building before the pathologist calls the results back? That's my favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Apr 25, 2024 at 3:31?PM Karen Schieberl CA-San Francisco < Karen.Schieberl at commonspirit.org> wrote: > This does apply to Frozen and Touch preps. > Karen Schieberl HT ASCP > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.schieberl@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > > On Thu, Apr 25, 2024 at 1:20?PM Jay Lundgren > wrote: > >> *USE CAUTION - EXTERNAL EMAIL* >> ------------------------------ >> You mean for frozens? >> >> On Thu, Apr 25, 2024 at 12:44?PM Karen Schieberl CA-San Francisco via >> Histonet wrote: >> >>> Good Morning, >>> I am not sure if there is a CAP or Joint commission Policy on pre making >>> slides for intraoperative consultation. Can someone let me know if there >>> is one? I have looked but was unable to find one. It just seems like >>> errors can be made especially if you have multiple patients or for some >>> reason the information on the slide does not match the patient. Any help >>> would be greatly appreciated. >>> >>> Thanks, >>> Karen Schieberl HT ASCP >>> >>> Lead Histology Technician >>> >>> St. Mary's Medical Center >>> >>> 450 Stanyan St. >>> >>> San Francisco, Ca. 94117 >>> >>> 415-750-5751 >>> >>> karen.schieberl@ commonspirit.org >>> >>> >>> >>> >>> Caution: This email message, including all content and attachments, is >>> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The >>> information contained in this email message is intended only for the use >>> of >>> the recipient(s) named above. If the reader of this message is not the >>> intended recipient or an agent responsible for delivering it to the >>> intended recipient, you have received this document in error. Any >>> further >>> review, dissemination, distribution, or copying of this message is >>> strictly >>> prohibited. If you have received this communication in error, please >>> notify us immediately by reply email. Thank you >>> >>> Caution: This email is both proprietary and confidential, and not >>> intended for transmission to (or receipt by) any unauthorized person(s). If >>> you believe that you have received this email in error, do not read any >>> attachments. Instead, kindly reply to the sender stating that you have >>> received the message in error. Then destroy it and any attachments. Thank >>> you. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >> From wcavett at umc.edu Thu Apr 25 17:30:22 2024 From: wcavett at umc.edu (Will Cavett) Date: Thu, 25 Apr 2024 22:30:22 +0000 Subject: [Histonet] [EXTERNAL]Re: Premaking slides In-Reply-To: References: Message-ID: Hello Karen: At my institution, which is a teaching facility and a level one trauma center, these are scheduled. The pathologists felt this was best for patient safety. The slides are labeled per procedure and not labeled before so we don?t have a lot of slides labeled. We have trained the staff collecting to inform us of location change when they come out with the sample. This helps our staff tremendously. I hope this helps. Will Cavett, II -----Original Message----- From: Jay Lundgren via Histonet Sent: Thursday, April 25, 2024 4:16 PM To: Karen Schieberl CA-San Francisco Cc: Histonet Subject: [EXTERNAL]Re: [Histonet] Premaking slides I mean, you're not going to hand the pathologist an unlabelled slide, obviously. This leaves you two options, label the slide before the tissue is on it, or afterward. Labelling the slide before is easier and quicker. I'm gonna label my slides *immediately* before cutting the frozen, or squishing the tissue. Patient name at the very least, if it hasn't been accessioned yet. But you don't want to leave any labeled blank slides laying around, that's asking for trouble. If you label too many slides, for instance, discard them immediately. If you are doing multiple cases at once, you are doing it wrong. I know a lot of places operate like this, but frozens are supposed to be scheduled for a reason. I mean, you might get an occasional emergency frozen for an ectopic pregnancy, but that's rare. There is a scheduler in surgery, they should be doing their job. Or maybe the head of Pathology needs to talk to the head of Surgery and explain how risky it is to be dealing with multiple frozens at once. Especially if it's the same tissue type. I'll probably get a lot of flak for this viewpoint, "Oh that's impossible at my institution!". But it's not. I've dealt with this as manager at several institutions and it can be fixed. It's not going to make you any friends in Surgery, but it's ultimately about patient safety. It's just that most hospitals are terrified of surgeons and will let them do whatever they want. How about the surgeon that leaves the building before the pathologist calls the results back? That's my favorite. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Apr 25, 2024 at 3:31?PM Karen Schieberl CA-San Francisco < Karen.Schieberl at commonspirit.org> wrote: > This does apply to Frozen and Touch preps. > Karen Schieberl HT ASCP > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.schieberl@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, > is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. > The information contained in this email message is intended only for > the use of the recipient(s) named above. If the reader of this message > is not the intended recipient or an agent responsible for delivering > it to the intended recipient, you have received this document in > error. Any further review, dissemination, distribution, or copying of > this message is strictly prohibited. If you have received this > communication in error, please notify us immediately by reply email. > Thank you > > > On Thu, Apr 25, 2024 at 1:20?PM Jay Lundgren > wrote: > >> *USE CAUTION - EXTERNAL EMAIL* >> ------------------------------ >> You mean for frozens? >> >> On Thu, Apr 25, 2024 at 12:44?PM Karen Schieberl CA-San Francisco via >> Histonet wrote: >> >>> Good Morning, >>> I am not sure if there is a CAP or Joint commission Policy on pre >>> making slides for intraoperative consultation. Can someone let me know if there >>> is one? I have looked but was unable to find one. It just seems like >>> errors can be made especially if you have multiple patients or for >>> some reason the information on the slide does not match the patient. >>> Any help would be greatly appreciated. >>> >>> Thanks, >>> Karen Schieberl HT ASCP >>> >>> Lead Histology Technician >>> >>> St. Mary's Medical Center >>> >>> 450 Stanyan St. >>> >>> San Francisco, Ca. 94117 >>> >>> 415-750-5751 >>> >>> karen.schieberl@ commonspirit.org >>> >>> >>> >>> >>> Caution: This email message, including all content and attachments, >>> is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. >>> The information contained in this email message is intended only for >>> the use of the recipient(s) named above. If the reader of this >>> message is not the intended recipient or an agent responsible for >>> delivering it to the intended recipient, you have received this >>> document in error. Any further review, dissemination, distribution, >>> or copying of this message is strictly prohibited. If you have >>> received this communication in error, please notify us immediately >>> by reply email. Thank you >>> >>> Caution: This email is both proprietary and confidential, and not >>> intended for transmission to (or receipt by) any unauthorized >>> person(s). If you believe that you have received this email in >>> error, do not read any attachments. Instead, kindly reply to the >>> sender stating that you have received the message in error. Then >>> destroy it and any attachments. Thank you. >>> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lis/ >>> ts.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C02%7 >>> Cwcavett%40umc.edu%7C114a86a8bd074fdd75a408dc656cff1e%7C78a0681ef0be >>> 47e280498616858818a5%7C0%7C0%7C638496806170633367%7CUnknown%7CTWFpbG >>> Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn >>> 0%3D%7C0%7C%7C%7C&sdata=axjzt7ETFB%2B6b8imYZf%2F5RcM9RTakJP7eVF8bkBx >>> JiI%3D&reserved=0 >>> >> rldefense.proofpoint.com%2Fv2%2Furl%3Fu%3Dhttp-3A__lists.utsouthwest >>> ern.edu_mailman_listinfo_histonet%26d%3DDwMFaQ%26c%3DYFhW2PYwN3hsZho >>> CqLOPHsIEIPQ6qDXkZ40AlEYUG9c%26r%3DfnkbvT8D25g0nM9qsmusaIIGXMRtGYAvO >>> TiZHWzeQ6Lb9v86eSROCr3zok0ftoBM%26m%3DeA5A20TalbYgTy_IUtHpfZKSkMDgrx >>> idp1yLTLOdzc_eUqtXw-WEoDUNIDfp67bn%26s%3Dm-Ly3i0Wyosg9X_182mk74S2YBl >>> EVEHVH-NM2S-O5oY%26e%3D&data=05%7C02%7Cwcavett%40umc.edu%7C114a86a8b >>> d074fdd75a408dc656cff1e%7C78a0681ef0be47e280498616858818a5%7C0%7C0%7 >>> C638496806170642819%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQ >>> IjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C0%7C%7C%7C&sdata=YKUr%2 >>> B9cHqlABfMqdZka%2Bc6U8YdWeCcyrQoA6dxfYjZ0%3D&reserved=0> >>> >> _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From wcavett at umc.edu Thu Apr 25 17:39:16 2024 From: wcavett at umc.edu (Will Cavett) Date: Thu, 25 Apr 2024 22:39:16 +0000 Subject: [Histonet] [EXTERNAL] Epredia xylene substitute In-Reply-To: <1779091641.2783754.1713985177046@mail.yahoo.com> References: <1779091641.2783754.1713985177046.ref@mail.yahoo.com> <1779091641.2783754.1713985177046@mail.yahoo.com> Message-ID: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -----Original Message----- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From thisisann at aol.com Thu Apr 25 19:07:01 2024 From: thisisann at aol.com (Ann Specian) Date: Fri, 26 Apr 2024 00:07:01 +0000 (UTC) Subject: [Histonet] [EXTERNAL] Epredia xylene substitute In-Reply-To: References: <1779091641.2783754.1713985177046.ref@mail.yahoo.com> <1779091641.2783754.1713985177046@mail.yahoo.com> Message-ID: <1866064876.3287859.1714090021960@mail.yahoo.com> Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel,?We are looking for Processing protocols that have already been tried and?true. If you have any,? if you could share them, it would be greatly appreciated. Sent from the all new AOL app for iOS On Thursday, April 25, 2024, 6:39 PM, Will Cavett wrote: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -----Original Message----- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. From gu.lang at gmx.at Sun Apr 28 01:19:14 2024 From: gu.lang at gmx.at (Gudrun Lang) Date: Sun, 28 Apr 2024 08:19:14 +0200 Subject: [Histonet] [EXTERNAL] Epredia xylene substitute In-Reply-To: <1866064876.3287859.1714090021960@mail.yahoo.com> References: <1779091641.2783754.1713985177046.ref@mail.yahoo.com> <1779091641.2783754.1713985177046@mail.yahoo.com> <1866064876.3287859.1714090021960@mail.yahoo.com> Message-ID: <001a01da9933$fe99b900$fbcd2b00$@gmx.at> Hi Ann, we have used ShellSol for years in our VIPs until our supplier stopped it. That's also a kind of naphta with aliphatic hydrogencarbons. After that wie changed to the Sakura-substitute with the same protocol. For standard processing we had/have two stations with 60 min each at 40 ?C. For fat processing the time is doubled. That works for our specimens well enough. Gudrun -----Urspr?ngliche Nachricht----- Von: Ann Specian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 26. April 2024 02:07 An: Will Cavett Cc: Histonet Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel, We are looking for Processing protocols that have already been tried and true. If you have any, if you could share them, it would be greatly appreciated. Sent from the all new AOL app for iOS On Thursday, April 25, 2024, 6:39 PM, Will Cavett wrote: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -----Original Message----- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From thisisann at aol.com Sun Apr 28 07:34:09 2024 From: thisisann at aol.com (Ann Specian) Date: Sun, 28 Apr 2024 12:34:09 +0000 (UTC) Subject: [Histonet] [EXTERNAL] Epredia xylene substitute In-Reply-To: <001a01da9933$fe99b900$fbcd2b00$@gmx.at> References: <1779091641.2783754.1713985177046.ref@mail.yahoo.com> <1779091641.2783754.1713985177046@mail.yahoo.com> <1866064876.3287859.1714090021960@mail.yahoo.com> <001a01da9933$fe99b900$fbcd2b00$@gmx.at> Message-ID: <1695214507.3836247.1714307649088@mail.yahoo.com> Thank you! On Sunday, April 28, 2024 at 02:19:24 AM EDT, Gudrun Lang wrote: Hi Ann, we have used ShellSol for years in our VIPs until our supplier stopped it. That's also a kind of naphta with aliphatic hydrogencarbons. After that wie changed to the Sakura-substitute with the same protocol. For standard processing we had/have two stations with 60 min each at 40 ?C. For fat processing the time is doubled. That works for our specimens well enough. Gudrun -----Urspr?ngliche Nachricht----- Von: Ann Specian via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Freitag, 26. April 2024 02:07 An: Will Cavett Cc: Histonet Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel, We are looking for Processing protocols that have already been tried and true. If you have any,? if you could share them, it would be greatly appreciated. Sent from the all new AOL app for iOS On Thursday, April 25, 2024, 6:39 PM, Will Cavett wrote: I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps. Will Cavett, II -----Original Message----- From: Ann Specian via Histonet Sent: Wednesday, April 24, 2024 2:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL][Histonet] Epredia xylene substitute We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share? Sent from the all new AOL app for iOS _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmargaryan88 at gmail.com Mon Apr 29 18:49:04 2024 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Mon, 29 Apr 2024 18:49:04 -0500 Subject: [Histonet] Alcohol Message-ID: Hello, What type of ALCOHOL ETHYL you?re using in your histology lab.? We are thinking to get 20-30-gal drum. Could you please help me with that? Your suggestion is appreciated, Naira From relia1 at earthlink.net Tue Apr 30 12:02:06 2024 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 30 Apr 2024 13:02:06 -0400 Subject: [Histonet] Hey Histopeeps! Are you READY for SUMMER? Here are some Recruiting Insider Tips to get you READY! Message-ID: <000001da9b20$222f12a0$668d37e0$@earthlink.net> Hello Histonetters, I hope you are having a great day! Can you believe it's already the beginning of May? SUMMER IS ALMOST HERE!!! It's that time of year for proms, graduations, weddings.... Major Life Changes... This time of year, also presents a unique opportunity for you as an employer. As we and our family members celebrate these changes it also inspires many of us to seek new horizons ourselves. I always hear from many histotechs this time of year who say it's time for: A new challenge A change of scenery A move closer to family Did I just speak with someone interested in your area! Histonetters! that's why I'm touching base, to see if you have any current or anticipated histology openings you need assistance with. I curate a nationwide network of over 9000 histology professionals that I communicate with regularly. I am aware of who among them is looking for a new position and who is considering a move. I know why they want to make a job change, where they want to live and what their credentials are. I don't advertise on the big job boards! Why duplicate your efforts? I rely on my contacts and expertise to enhance your hiring process. If you would like: Assistance with an immediate need or Would like to discuss working with me in the future, or Would like for me to send some info on my services, For more information You can contact me. Toll Free: 866-607-3542 Cell/Text: 407-353-5070 email: relia1 at earthlink.net Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net From jaylundgren at gmail.com Tue Apr 30 12:08:03 2024 From: jaylundgren at gmail.com (Jay Lundgren) Date: Tue, 30 Apr 2024 12:08:03 -0500 Subject: [Histonet] Alcohol In-Reply-To: References: Message-ID: Call StatLab, 1-800-442-3573 On Mon, Apr 29, 2024 at 6:49?PM Naira Margaryan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > What type of ALCOHOL ETHYL you?re using in your histology lab.? > > We are thinking to get 20-30-gal drum. > > Could you please help me with that? > > > > Your suggestion is appreciated, > > Naira > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From john at imebinc.com Tue Apr 30 12:49:25 2024 From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=) Date: Tue, 30 Apr 2024 10:49:25 -0700 Subject: [Histonet] Histonet Digest, Vol 245, Issue 20 In-Reply-To: References: Message-ID: Ethyl Alcohol, Reagent 100% alcohol is what is normally used in Pathology processing and slide staining IMEB Inc offer all grades of Reagent alcohol, pure ethyl is a controlled alcohol regulated and taxed by government Regards John > On Apr 30, 2024, at 10:18?AM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Alcohol (Naira Margaryan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Mon, 29 Apr 2024 18:49:04 -0500 > From: Naira Margaryan > To: Histonet > Subject: [Histonet] Alcohol > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hello, > > What type of ALCOHOL ETHYL you?re using in your histology lab.? > > We are thinking to get 20-30-gal drum. > > Could you please help me with that? > > > > Your suggestion is appreciated, > > Naira > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 245, Issue 20 > *****************************************