[Histonet] Detergent in heating antigen retrieval

[Alonso Martínez Canabal]

Dear histoneters,
           I have performed heating antigen retrieval with citrate buffer
pH 6 with 0.05% tween-20, however I have seem recipes  with no detergent,
anyone has any experience or knowledge if it is better with or without the
detergent?
          Thank you!

-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833