From relia1 at earthlink.net Wed Sep 6 11:32:58 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 6 Sep 2023 12:32:58 -0400 Subject: [Histonet] RELIA HOT Job Alert!! Exciting NEW Opportunity - Mohs in Arizona!! Message-ID: <031801d9e0df$ccbede90$663c9bb0$@earthlink.net> RELIA HOT JOB ALERT!!! Mohs Tech needed in Arizona!! Histopeeps! Ever thought of moving to AZ? Do you love Mohs or want to do more Mohs? This might be the position for you! For more info: email me - relia1 at earthlink.net or cell/text: 407-353-5070 #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From criley at udel.edu Thu Sep 7 15:34:47 2023 From: criley at udel.edu (Charles Riley) Date: Thu, 7 Sep 2023 16:34:47 -0400 Subject: [Histonet] Alizaren Red and von kossa staining Message-ID: Does anyone have any methods to soften bone without decalifier solutions to perform microtomy for these tests on E 18.5, P7, and 930 mouse skulls From michael.gudo at morphisto.de Fri Sep 8 02:36:18 2023 From: michael.gudo at morphisto.de (Dr. Michael Gudo (Morphisto GmbH)) Date: Fri, 8 Sep 2023 09:36:18 +0200 Subject: [Histonet] Alizaren Red and von kossa staining In-Reply-To: References: Message-ID: Dear Charles, Can you provide more details about your needs? We might have gentler decalcifying solutions than the usual ones. Do you need histology services for your mouse skulls? We can handle a wide range of routine and special histological needs. Could you tell us where on the skull you plan to use Alizarin Red and Kossa Staining? Best, Michael > Am 07.09.2023 um 22:34 schrieb Charles Riley via Histonet : > > Does anyone have any methods to soften bone without decalifier solutions to > perform microtomy for these tests on E 18.5, P7, and 930 mouse skulls > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ******************************************************************************************************** MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Schumannstrasse 144 63069 Offenbach am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.gudo at morphisto.de Internet: http://www.morphisto.de/ Vertretungsberechtigter Gesch?ftsf?hrer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gem?? ? 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. From Nancy.Schmitt at mercyhealth.com Tue Sep 12 12:07:25 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Tue, 12 Sep 2023 17:07:25 +0000 Subject: [Histonet] Mohs Message-ID: Hello- Is there any kind of grandfathering for HT certificate people cutting Mohs? The surgeon does all inking and cutting. Thank you Nancy Schmitt, MLT(ASCP) CM HT CM Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From patpxs at gmail.com Tue Sep 12 21:11:14 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Wed, 13 Sep 2023 02:11:14 +0000 (UTC) Subject: [Histonet] Formalin pH References: <517524818.599308.1694571074195.ref@mail.yahoo.com> Message-ID: <517524818.599308.1694571074195@mail.yahoo.com> Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello From bcooper at chla.usc.edu Tue Sep 12 21:19:08 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 13 Sep 2023 02:19:08 +0000 Subject: [Histonet] Formalin pH In-Reply-To: <517524818.599308.1694571074195@mail.yahoo.com> References: <517524818.599308.1694571074195.ref@mail.yahoo.com>, <517524818.599308.1694571074195@mail.yahoo.com> Message-ID: Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: Paula Sicurello via Histonet Sent: Tuesday, September 12, 2023 7:12:05 PM To: HistoNet Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From jkiernan at uwo.ca Tue Sep 12 22:58:24 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 13 Sep 2023 03:58:24 +0000 Subject: [Histonet] Formalin pH In-Reply-To: <517524818.599308.1694571074195@mail.yahoo.com> References: <517524818.599308.1694571074195.ref@mail.yahoo.com> <517524818.599308.1694571074195@mail.yahoo.com> Message-ID: You can check the pH with a pH meter. This is the most accurate way, but the meter's electrode must be calibrated against at least two standard (usually bought) buffer solutions, such as 4.0 and 7.0. If you don't have a pH meter or the know-how to use one properly, you can use indicator papers, which are inexpensive but deteriorate with storage. With a few "books" of indicator papers covering the range 3 to 8 you should be able to get a value accurate to the nearest 0.2 pH units. I'm assuming that by "formalin" you mean the concentrated solution that is nominally 37-40% by weight of formaldehyde gas in water. Its pH falls with time. For more information about formalin, see https://www.cambridge.org/core/services/aop-cambridge-core/content/view/911F1870567E2487D56A51140EB8EA17/S1551929500057060a.pdf/div-class-title-formaldehyde-formalin-paraformaldehyde-and-glutaraldehyde-what-they-are-and-what-they-do-div.pdf. It's a free download, with >600 citations according to Google Scholar. John Kiernan Emeritus, UWO, London, Canada = = = ________________________________ From: Paula Sicurello via Histonet Sent: September 12, 2023 10:11 PM To: HistoNet Subject: [Histonet] Formalin pH Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ewj at pigs.ag Wed Sep 13 07:22:37 2023 From: ewj at pigs.ag (ewj at pigs.ag) Date: Wed, 13 Sep 2023 20:22:37 +0800 Subject: [Histonet] Formalin pH In-Reply-To: References: <517524818.599308.1694571074195.ref@mail.yahoo.com> , <517524818.599308.1694571074195@mail.yahoo.com> Message-ID: Sooner or later the people have got to rise up? and tell those brain-dead paper-shuffling regulators to just stuff it. ?Their mindless interference is? just wasting time and interfering with people's ability to keep their own garden. -----Original Message----- From: Cooper, Brian via Histonet Reply-To: Cooper, Brian To: Paula Sicurello, HistoNet Subject: Re: [Histonet] Formalin pH Date: Today 10:19 AM Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: Paula Sicurello via Histonet Sent: Tuesday, September 12, 2023 7:12:05 PM To: HistoNet Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Hello Histoteckies, What are y'all doing regarding the CAP requirement to monitor the pH of formalin? We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. Looking forward to your replies. Toodles! Sincerely, Paula Sicurello _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From beth.oneil1 at wvumedicine.org Wed Sep 13 07:17:53 2023 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth) Date: Wed, 13 Sep 2023 12:17:53 +0000 Subject: [Histonet] Fw: Reminder Anatomic Pathology Rounds today Wednesday 9/13 In-Reply-To: References: Message-ID: From: Smittle, Anne Sent: Wednesday, September 13, 2023 7:59 AM Subject: FW: Reminder Anatomic Pathology Rounds today Wednesday 9/13 Good morning, MS2 PALM Rounds today beginning around 1:00ish today in the Gross Room, continuing on into Histology Labs and finishing up in the Residents Library looking at current cases with Dr. Williams. Thank you for your assistance with the med school students ---next week two Rounds on Tuesday and Wednesday, Anne Smittle PALM Course Coordinator West Virginia University 2190 HSCN Morgantown, WV 26505 (304) 293-3779 asmittle at hsc.wvu.edu -----Original Message----- From: asmittle at hsc.wvu.edu Sent: Wednesday, September 13, 2023 7:57 AM To: Smittle, Anne Subject: Reminder Anatomic Pathology Rounds today 9/13 Reminder AP Rounds today. Check instructions on the Sign Up List for AP Rounds. Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From kdean70 at hotmail.com Wed Sep 13 12:24:20 2023 From: kdean70 at hotmail.com (Ken M) Date: Wed, 13 Sep 2023 17:24:20 +0000 Subject: [Histonet] IHC/Special staining Message-ID: Does anyone know of any lab with excess capacity good quality and fast turnaround that could do IHC/special staining on the side? We are out of capacity, and we don't always have the antibodies that we need. Kevin kdean70 at hotmail.com From Charles.Bacon at baystatehealth.org Wed Sep 13 15:31:56 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Wed, 13 Sep 2023 20:31:56 +0000 Subject: [Histonet] Formalin pH In-Reply-To: References: Message-ID: We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "ewj at pigs.ag" wrote: > > ?Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -----Original Message----- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcooper at chla.usc.edu > > ________________________________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePelpt2pjY$ > > From afhenwood at outlook.com Wed Sep 13 18:01:53 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Wed, 13 Sep 2023 23:01:53 +0000 Subject: [Histonet] Formalin pH In-Reply-To: References: Message-ID: Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous ?smell? test. At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Other advantages are that the fixative is more readily distinguished from other colourless solutions such as saline, thus preventing accidental misuse, and formalin spills are more easily recognised. Hew-Shue N (1991) ?Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin? J Histotechnol 14(4):257-260. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Bacon, Charles via Histonet Sent: Thursday, 14 September 2023 6:42 AM To: ewj at pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "ewj at pigs.ag" wrote: > > ?Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -----Original Message----- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcooper at chla.usc.edu > > ________________________________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePelpt2pjY$ > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kelly.Pairan at ohiohealth.com Thu Sep 14 08:57:53 2023 From: Kelly.Pairan at ohiohealth.com (Pairan, Kelly) Date: Thu, 14 Sep 2023 13:57:53 +0000 Subject: [Histonet] Small Inexpensive Stainer for Special Stains Message-ID: Good Morning, One of my sites is looking to automate a few of their specials stains. They do not have a high throughput and are looking for something small, reliable and budget friendly.They are not looking for something as high tech as an Artisan or a Benchmark at this time. They are looking to automate Masson's Trichrome, Giemsa, PAS-F and Iron right now. Does anyone know of an instrument that meets this criteria? Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist Anatomic Pathology and Cytology OhioHealth Laboratory Systems Suite 210 North Medical Building 3535 Olentangy River Rd Columbus, OH 43214 Email: kelly.pairan at ohiohealth.com Work: (614) 566-3575 Cell: (614) 312-0104 From pslath at gwu.edu Thu Sep 14 09:37:32 2023 From: pslath at gwu.edu (Patricia Latham) Date: Thu, 14 Sep 2023 10:37:32 -0400 Subject: [Histonet] Small Inexpensive Stainer for Special Stains In-Reply-To: References: Message-ID: Thank you for asking - I am also looking for recommendations on a small stainer. Pat Latham On Thu, Sep 14, 2023 at 9:58?AM Pairan, Kelly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, > One of my sites is looking to automate a few of their specials stains. > They do not have a high throughput and are looking for something small, > reliable and budget friendly.They are not looking for something as high > tech as an Artisan or a Benchmark at this time. They are looking to > automate Masson's Trichrome, Giemsa, PAS-F and Iron right now. Does anyone > know of an instrument that meets this criteria? > > Thanks, > Kelly > > Kelly Pairan, HT(ASCP)CM, HQIPCM > Technical Scientist Anatomic Pathology and Cytology > OhioHealth Laboratory Systems > Suite 210 North Medical Building > 3535 Olentangy River Rd > Columbus, OH 43214 > > Email: kelly.pairan at ohiohealth.com > Work: (614) 566-3575 > Cell: (614) 312-0104 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Thu Sep 14 09:59:22 2023 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 14 Sep 2023 14:59:22 +0000 (UTC) Subject: [Histonet] Small Inexpensive Stainer for Special Stains In-Reply-To: References: Message-ID: <233834317.1287042.1694703562119@mail.yahoo.com> Just a thought, but H&E stainers have multiple programs to code. Having a second machine to use for specials would be an option. Maybe even bring slides to water and use different rows of containers for different stains. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, September 14, 2023 at 09:52:10 AM CDT, Patricia Latham via Histonet wrote: Thank you for asking - I am also looking for recommendations on a small stainer. Pat Latham On Thu, Sep 14, 2023 at 9:58?AM Pairan, Kelly via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Good Morning, > One of my sites is looking to automate a few of their specials stains. > They do not have a high throughput and are looking for something small, > reliable and budget friendly.They are not looking for something as high > tech as an Artisan or a Benchmark at this time.? They are looking to > automate Masson's Trichrome, Giemsa, PAS-F and Iron right now.? Does anyone > know of an instrument that meets this criteria? > > Thanks, > Kelly > > Kelly Pairan, HT(ASCP)CM, HQIPCM > Technical Scientist Anatomic Pathology and Cytology > OhioHealth Laboratory Systems > Suite 210 North Medical Building > 3535 Olentangy River Rd > Columbus, OH 43214 > > Email: kelly.pairan at ohiohealth.com > Work: (614) 566-3575 > Cell: (614) 312-0104 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.tague at pathologyarts.com Thu Sep 14 13:18:26 2023 From: c.tague at pathologyarts.com (Curt Tague) Date: Thu, 14 Sep 2023 18:18:26 +0000 Subject: [Histonet] Old blocks for IHC controls Message-ID: Forgive me if this has already been discussed ad nauseum... what are your thoughts on using old blocks for IHC control, like normal breast for CK7 or AE1/AE3... if we test the tissue and it works is that sufficient or is there some added steps and requirements I should be aware of? We have blocks up to 15 years old, looks like there is a lot of good tissue we can use if age isn't an issue. Best and thanks! Curt From scrochiere at hotmail.com Fri Sep 15 15:02:44 2023 From: scrochiere at hotmail.com (Steven Crochiere) Date: Fri, 15 Sep 2023 20:02:44 +0000 Subject: [Histonet] Retic help Message-ID: Any advice on getting my retic stain to work properly is appreciated. Currently coming out a washed out gray overall, with proper staining at the periphery of the section. Thanks Sent via the Samsung Galaxy, an AT&T 4G LTE smartphone Get Outlook for Android From jkiernan at uwo.ca Sat Sep 16 00:50:15 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 16 Sep 2023 05:50:15 +0000 Subject: [Histonet] Formalin pH In-Reply-To: References: Message-ID: Thank you Tony, for drawing attention to that excellent 1991 paper in the Journal of Histotechnology. It was published in the years when the NSH's journal was not included in Current Contents and was not taken by most libraries. Sadly, Norman Hew-Shue died in Toronto in 2022. Papers in J. Histotechnol. (including all back-issues) are now easily available as PDF downloads from libraries that subscribe to the many journals published by Taylor and Francis. That's probably most academic and biomedical libraries. Members of the NSH still get the paper journal and have access to the archive by way of an obscure members-only link on the society's web site; it is very difficult to find. Why won't the NSH make its journal conspicuous and easily accessible to all by way of a single click from https://www.nsh.org/home? Grrr! John Kiernan. = = = ________________________________ From: Tony Henwood via Histonet Sent: September 13, 2023 7:01 PM To: Bacon, Charles ; ewj at pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous ?smell? test. At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Other advantages are that the fixative is more readily distinguished from other colourless solutions such as saline, thus preventing accidental misuse, and formalin spills are more easily recognised. Hew-Shue N (1991) ?Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin? J Histotechnol 14(4):257-260. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Bacon, Charles via Histonet Sent: Thursday, 14 September 2023 6:42 AM To: ewj at pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "ewj at pigs.ag" wrote: > > ?Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -----Original Message----- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcooper at chla.usc.edu > > ________________________________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePelpt2pjY$ > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Tony Henwood via Histonet Sent: September 13, 2023 7:01 PM To: Bacon, Charles ; ewj at pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous ?smell? test. At an acidic pH (5.2) the intense indicator colour is yellow whereas at pH 6.8, the colour is purple. A saturated solution of the dye is prepared, and 2 to 4 drops are added to 10 litres of neutral buffered formalin. Other advantages are that the fixative is more readily distinguished from other colourless solutions such as saline, thus preventing accidental misuse, and formalin spills are more easily recognised. Hew-Shue N (1991) ?Bromcresol Purple as a Colored Marker and pH Indicator for Ten Percent Neutral Buffered Forrnalin? J Histotechnol 14(4):257-260. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Bacon, Charles via Histonet Sent: Thursday, 14 September 2023 6:42 AM To: ewj at pigs.ag Cc: HistoNet Subject: Re: [Histonet] Formalin pH We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "ewj at pigs.ag" wrote: > > ?Sooner or later the people have got to rise up > and tell those brain-dead paper-shuffling regulators > to just stuff it. Their mindless interference is > just wasting time and interfering with people's > ability to keep their own garden. > > -----Original Message----- > From: Cooper, Brian via Histonet > Reply-To: Cooper, Brian > To: Paula Sicurello, HistoNet > Subject: Re: [Histonet] Formalin pH > Date: Today 10:19 AM > > Ask your vendor to send a certificate of analysis that includes the pH. That's what we did and now they come with each lot. We do have to bug them for them occasionally. > > I'm betting your vendor has at least heard about this from other customers so it should be on their radar at the very least. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcooper at chla.usc.edu > > ________________________________ > From: Paula Sicurello via Histonet > Sent: Tuesday, September 12, 2023 7:12:05 PM > To: HistoNet > Subject: [Histonet] Formalin pH (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** > This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/1dY8pbLOSpzQKfWx-yvsO5rujytRiLi5FacpaIq-afFO3eWHoj801ibzodIvkn6kWJvH0G2K0mQEZsm-bweaI-0WiwrKEp9XDD-CIrZCerqewH6wfWut7f2aExx7-0Qp1t67zvWQEZovO1QSD1rx0Pyyf0mc80Xc9xXtgj0R1PDbB8vEDPEepcTWafNdNrZKsWKkUwZlF-2tvTQzsn9RxxaQkzzP3rKjsm9Sd35YA9q2N3LM1LbKMIK3GMBAEFn-4HOieXd2nYDQiB3I8SNmwcRDaEoCsyfVtXFrBlJaPmty9xCB6U_ao9WhiJsiDs0mKiphCHZl4SRW8cWwawwDQfNLVDcfj-ajpmEc1E_8mhQwbpcLwmjVDpeOEm7JKweDGFRg3cAe7cz-_6LgHJAxTkvDl6B6uUzM9ak7s-E1aIYOLRgnPn61BtAnF5gQ6ncGtrYsdaqVIpAjc_3ZQEHXIGQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePe6Pt9wgc$ > > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!YyUGwYyrMdYlhUlXnDMq68DTxOX1KGg3ZgSeT5GCab42ZrSt48hyrjyqzddx8zyiZ1dZlpGtq26xTL_0DeXre8ERyLwEfuZBEZQRRePelpt2pjY$ > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Bacon at baystatehealth.org Sat Sep 16 14:14:31 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Sat, 16 Sep 2023 19:14:31 +0000 Subject: [Histonet] Retic help In-Reply-To: References: Message-ID: Hi Steven, We ran into this issue and it was the pH of the water. We were using our sterile line from the facility but the pH was too high. We used tap and it worked fine. This will also effect your stock solutions if you make them yourself so be aware of that as well. Sent from my iPhone > On Sep 15, 2023, at 4:02 PM, Steven Crochiere wrote: > > ?Any advice on getting my retic stain to work properly is appreciated. Currently coming out a washed out gray overall, with proper staining at the periphery of the section. Thanks > > Sent via the Samsung Galaxy, an AT&T 4G LTE smartphone > Get Outlook for Android > From mashalsmom at gmail.com Sat Sep 16 17:43:55 2023 From: mashalsmom at gmail.com (Emily Horst) Date: Sat, 16 Sep 2023 18:43:55 -0400 Subject: [Histonet] Formalin pH In-Reply-To: <517524818.599308.1694571074195@mail.yahoo.com> References: <517524818.599308.1694571074195.ref@mail.yahoo.com> <517524818.599308.1694571074195@mail.yahoo.com> Message-ID: Yes we are doing the same thing as Brian?s lab. Getting the certificates from the vendor. Emily Horst On Tue, Sep 12, 2023 at 10:17 PM Paula Sicurello via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello Histoteckies, > What are y'all doing regarding the CAP requirement to monitor the pH of > formalin? > We buy tons and tons of the 5 gallon cubitainers and we are still debating > over how to check the pH. > > Looking forward to your replies. > Toodles! > Sincerely, > > Paula Sicurello > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From VKurth at uwhealth.org Mon Sep 18 07:37:38 2023 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Mon, 18 Sep 2023 12:37:38 +0000 Subject: [Histonet] Retic help In-Reply-To: References: Message-ID: Same here, we moved to a new location and the PH of the water was too acidic, the DI water would remove the silver and leave it yellowed. Some solutions that I was making that usually would take hours to go into solution on the stir plate went immediately. Good Luck! Ginny Kurth, HT, (ASCP)CM -----Original Message----- From: Bacon, Charles via Histonet Sent: Saturday, September 16, 2023 2:15 PM To: Steven Crochiere Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Retic help WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hi Steven, We ran into this issue and it was the pH of the water. We were using our sterile line from the facility but the pH was too high. We used tap and it worked fine. This will also effect your stock solutions if you make them yourself so be aware of that as well. Sent from my iPhone > On Sep 15, 2023, at 4:02 PM, Steven Crochiere wrote: > > ?Any advice on getting my retic stain to work properly is appreciated. > Currently coming out a washed out gray overall, with proper staining > at the periphery of the section. Thanks > > Sent via the Samsung Galaxy, an AT&T 4G LTE smartphone Get Outlook for > Android F%2Furldefense.com%2Fv3%2F__https%3A%2F%2Faka.ms%2FAAb9ysg__%3B!!P_vj- > BUkwjF5!dOCebh0fsgbQLocXqZQoEkoPW29mjmeyWFMjdmTqxRxruZpOFJeoFUn2zBThp7 > _vjNri_BS80eZ93nTL4cvhj2wvSg733KSLdYSW6rRFJZR9dJI%24&data=05%7C01%7Cvk > urth%40uwhealth.org%7C93920bbfc9de414d4f7d08dbb6e93ecf%7C0fd7902a3b4f4 > 9b0b1edaaa4d2b4f5f1%7C0%7C0%7C638304885145340771%7CUnknown%7CTWFpbGZsb > 3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D% > 7C3000%7C%7C%7C&sdata=XXvYoIirNxenSMzwNALbSCVl2IIfU98jrSPYUrXBehU%3D&r > eserved=0 > > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Mon Sep 18 11:27:12 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 18 Sep 2023 12:27:12 -0400 Subject: [Histonet] Travel Histology - Here's the SCOOP! Message-ID: <042701d9ea4c$fb5d2560$f2177020$@earthlink.net> Hi Histopeeps! Travel Histology - Here's The Scoop! I have exciting news. I just published my article on Travel Histology If you would like to read it just shoot me an email to relia1 at earthlink.net and I will send it to you. Wanna network with your fellow histotechs? Join my group on Facebook! www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From acanabal at ciencias.unam.mx Tue Sep 19 20:07:04 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Tue, 19 Sep 2023 19:07:04 -0600 Subject: [Histonet] p17 mice brain sections Message-ID: Hello, Have a great afternoon. I have done HAR with citrate buffer (pH 6.0) with tween, basically all my professional life of some 15 years. Several publications, using mice brain sections 40-50 microns thickness from cryostat (30% sucrose). Today I tried to do some p17 brain sections and the sections did not only fell off, but were completely destroyed. That never happened to me, I am wonder if anyone can have any idea of what happened? Thank you so much -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From rmccormick10 at yahoo.com Wed Sep 20 10:39:33 2023 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Wed, 20 Sep 2023 15:39:33 +0000 (UTC) Subject: [Histonet] Long term museum specimen storage References: <1566968759.3666182.1695224373409.ref@mail.yahoo.com> Message-ID: <1566968759.3666182.1695224373409@mail.yahoo.com> Hi All, I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in them). The specimens are currently in Jore's Fixative:?100 mL Distilled water 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium Chloride Preserving specimens is new to me. I've never heard of Jore's fixative before and I'm wondering if I could?get some advice, please???Do these specimens need to be replaced with the?same?solution? Could we rinse the specimen and replace the solution with 70% Alcohol? OR would 10% NBF be better to store the specimens in (or something al together different)? We have a varying display of specimens - anywhere from a small porcine optic nerve to a large equine granulosa cell tumor. Realizing it may be different based on the size of the specimen, approximately how often should the solution be changed ? Thank you so much! Any help or insight is much appreciated.? Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm Histology Diagnostic?Lab Supervisor College of Veterinary Medicine Texas A&M University From acanabal at ciencias.unam.mx Wed Sep 20 14:24:55 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Wed, 20 Sep 2023 13:24:55 -0600 Subject: [Histonet] Detergent in heating antigen retrieval Message-ID: Dear histoneters, I have performed heating antigen retrieval with citrate buffer pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, anyone has any experience or knowledge if it is better with or without the detergent? Thank you! -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From edmartin26 at gmail.com Wed Sep 20 20:24:14 2023 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 20 Sep 2023 21:24:14 -0400 Subject: [Histonet] Histonet Digest, Vol 237, Issue 4 In-Reply-To: References: Message-ID: Thought on alternative for Sudan Black. I don't use this stain...an alternative is an Oil Red O stain. Oil Red O is done on frozen sections...but you can also deparaffinize FFPE sections to water, and then perform your Oil Red O stain. I hope this helps. Very Respectfully, Eddie Martin Eddie Martin, HTL, QIHC The National Institutes of Health Bone Marrow Service 10 Center Drive Building 10, Room 2C360 Bethesda, MD 20892 Office: 301-594-2054 On Thu, Aug 10, 2023 at 12:59?PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Happy Hump Day Histopeeps!! Summer is Almost OVER!!! Here are > some exciting new opportunities for you and your friends! > (relia1 at earthlink.net) > 2. Sudan Black B (Betsy Molinari) > > > > ---------- Forwarded message ---------- > From: > To: > Cc: > Bcc: > Date: Wed, 9 Aug 2023 14:13:39 -0400 > Subject: [Histonet] Happy Hump Day Histopeeps!! Summer is Almost OVER!!! > Here are some exciting new opportunities for you and your friends! > Happy Hump Day Histopeeps!! > > It is hard to believe the summer is almost over. The kids are going back > to school and Labor Day is just a few weeks away! > > A few more weeks after that ?Halloween; Then Thanksgiving, Christmas, and > New Year?s Day!! > > ? Looking for a new job in the Fall? > > ? Planning a job change after the holidays? > > ? Considering making a move in 2024? > > Let?s Get The Ball Rolling!!! > > Histonetters! > > **Are you a histo tech looking for something better? > > **Are you a new or recent graduate of a histology school? > > **Are you a traveler transitioning into a permanent position? > > **Are you a Histotech looking to advance into leadership? > > **Are you a Supervisor looking to advance to lab management? > > Let?s Get The Ball Rolling!!! > > All you have to do is contact me at relia1 at earthlink.net > or call/text me at 407-353-5070 or call me > at > 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. > > > > If you know of anyone else who might be interested in subscribing to > RELIA?s Histology Careers Bulletin, please feel free to pass this along to > them. > > I have exciting opportunities in: > > ? Leadership > > ? Tech Support > > ? Mohs > > ? GI > > ? Derm > > ? Clinical > > ? AP > > ? Research > > These Opportunities are located in: > > ? Florida ? Brand New Lab! > > ? Washington > > ? South Carolina > > ? Arizona > > ? California > > ? Massachusetts > > ? Wisconsin > > ? Tennessee > > ? Georgia > > ? Alabama > > I invite you to join my group on Facebook: > > www.facebook.com/groups/histotechnologists > > > Have a Great Week! > > > > > > Thanks-Pam > > Right Time, Right Place, Right Move with RELIA! > > Providing excellent service exclusively to the Histology Community! > > Thank You! > > Pam M. Barker > > Pam Barker > > President/Senior Recruiting Specialist-Histology > > RELIA Solutions > > Specialists in Allied Healthcare Recruiting > > 5717 Red Bug Lake Road #330 > > Winter Springs, FL 32708-4969 > > Phone: (407)657-2027 > > Cell: (407)353-5070 > > FAX: (407)678-2788 > > Toll free: (866)60RELIA or (866)607-3542 > > E-mail: relia1 at earthlink.net > > https://www.facebook.com/RELIASolutionsforhistologyprofessionals > > www.linkedin.com/in/reliasolutions > > > I invite you to join my group on Facebook: > > www.facebook.com/groups/histotechnologists > > > Follow my hashtags to make your day great and your career greater! > > #ilovemyhistopeeps > > #jobs4myhistopeeps > > #histologyiscool > > #histologyjobs > > #histologycareers > > #histology > > > > > > > > > > > > ---------- Forwarded message ---------- > From: Betsy Molinari > To: Histonet > Cc: > Bcc: > Date: Thu, 10 Aug 2023 14:57:31 +0000 > Subject: [Histonet] Sudan Black B > Hi, > I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. > The biopsy is in a paraffin block. They are looking to better report and > understand the IHC. I am totally unfamiliar with this stain. I did some > reading but have been unable to find a protocol for paraffin sections. I > found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't > have that edition. Any ideas would be greatly appreciated . > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 1101 Bates St. > Houston, Texas 77030 > 832-355-6524 > > Betsy Molinari, HT (ASCP) > Sr. Histology Research Technician > CV Pathology Research > > The Texas Heart Institute (r) > 6770 Bertner Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: BMolinari at texasheart.org > texasheart.org | texasheartmedical.org< > https://www.texasheartmedical.org/> | facebook< > https://www.facebook.com/Texas.Heart.Institute> | twitter< > https://twitter.com/Texas_Heart> > > CONFIDENTIALITY NOTICE: This email and attachments contain information > that may be confidential or privileged. If you are not the intended > recipient, notify the sender at once and delete this message completely > from your information system. Further use, disclosure, or copying of > information contained in this email is not authorized, and any such action > should not be construed as a waiver of privilege or other confidentiality > protections. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Thu Sep 21 00:20:13 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 21 Sep 2023 05:20:13 +0000 Subject: [Histonet] Long term museum specimen storage In-Reply-To: <1566968759.3666182.1695224373409@mail.yahoo.com> References: <1566968759.3666182.1695224373409.ref@mail.yahoo.com> <1566968759.3666182.1695224373409@mail.yahoo.com> Message-ID: I don't know anything about "Jore's fixative" or the rationale of using a very hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride and sulphate ions. If brown stuff is now bleeding out of your museum specimens, Jore Juice evidently isn't a good preservative. According to Chapter 26 in the late Charles Culling's excellent book (3rd edn 1974; ISBN: 0407729011) the fixative/preservative for a museum specimen is optimized to preserve colour, which is the red or reddish-brown of haemoglobin and myoglobin. This usually is achieved with Kaiserling's fluid, which contains formalin, potassium acetate and also potassium nitrate (1.5% w/v) as an oxidant. Another approach involves treating specimens with carbon monoxide to convert all haemoglobin etc to a red carboxy derivative. If your museum specimens have already lost all their meaningful colours, a neutral buffered aqueous formaldehyde may be the best that you can provide to preserve the sizes and shapes. 70% alcohol will cause some shrinkage, and it may not be as easy to seal this solvent into a museum container as a watery diluted formalin. John Kiernan = = = ________________________________ From: Rhonda McCormick via Histonet Sent: September 20, 2023 11:39 AM To: Histonet Subject: [Histonet] Long term museum specimen storage Hi All, I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in them). The specimens are currently in Jore's Fixative: 100 mL Distilled water 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium Chloride Preserving specimens is new to me. I've never heard of Jore's fixative before and I'm wondering if I could get some advice, please? Do these specimens need to be replaced with the same solution? Could we rinse the specimen and replace the solution with 70% Alcohol? OR would 10% NBF be better to store the specimens in (or something al together different)? We have a varying display of specimens - anywhere from a small porcine optic nerve to a large equine granulosa cell tumor. Realizing it may be different based on the size of the specimen, approximately how often should the solution be changed ? Thank you so much! Any help or insight is much appreciated. Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm Histology Diagnostic Lab Supervisor College of Veterinary Medicine Texas A&M University _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu Sep 21 06:28:58 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 21 Sep 2023 13:28:58 +0200 Subject: [Histonet] Detergent in heating antigen retrieval In-Reply-To: References: Message-ID: <001001d9ec7e$d2102c50$763084f0$@gmx.at> Hi, I think the answer is as so often: it depends. Detergens solves membranes partly and leads to a higher permeabilization of the tissue. Some antigens may take advantage of that, some may not need it. Higher permeability is good for detection with high molecular complexes. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Alonso Mart?nez Canabal via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 20. September 2023 21:25 An: Histonet Betreff: [Histonet] Detergent in heating antigen retrieval Dear histoneters, I have performed heating antigen retrieval with citrate buffer pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, anyone has any experience or knowledge if it is better with or without the detergent? Thank you! -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks at gmail.com Thu Sep 21 11:40:40 2023 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 21 Sep 2023 12:40:40 -0400 Subject: [Histonet] p17 mice brain sections (Alonso Mart?nez Canabal) In-Reply-To: References: Message-ID: Hi, The thicker the section the more likely it will be to fall off. Frozen sections already love to fall off slides. You should cut them a lot thinner. 4 to 10 um should be the thickness, especially for frozens. If you want very thick sections as you describe, you would be better off cutting them and transferring them directly to an 8 well plate with PBS and doing the IHC as a floating section. Cheers, Amos Brooks > > Message: 1 > Date: Tue, 19 Sep 2023 19:07:04 -0600 > From: Alonso Mart?nez Canabal > To: Histonet > Subject: [Histonet] p17 mice brain sections > Message-ID: > wDugsDPPX5YTkxXUaipmwctzjcAZwf_d4A at mail.gmail.com> > Content-Type: text/plain; charset="UTF-8" > > Hello, > Have a great afternoon. I have done HAR with citrate buffer (pH 6.0) > with tween, basically all my professional life of some 15 years. Several > publications, using mice brain sections 40-50 microns thickness from > cryostat (30% sucrose). Today I tried to do some p17 brain sections and the > sections did not only fell off, but were completely destroyed. > That never happened to me, I am wonder if anyone can have any idea of > what happened? > > Thank you so much > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > From annigyg at gmail.com Thu Sep 21 12:36:48 2023 From: annigyg at gmail.com (Anne van Binsbergen) Date: Thu, 21 Sep 2023 19:36:48 +0200 Subject: [Histonet] Jores fluid - Histonet Digest, Vol 238, Issue 10 In-Reply-To: References: Message-ID: Hello gang Jores has several references found in a Google search, including a post right here on the Histonet from 2002. It contains Chloral Hydrate which is very difficult to find. Do a search and have a look. Several suggestions. AnnieinArabia (now retired in Africa) Sent from my iPhone > On 21 Sep 2023, at 7:07?PM, histonet-request at lists.utsouthwestern.edu wrote: > > ?Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Detergent in heating antigen retrieval (Alonso Mart?nez Canabal) > 2. Re: Histonet Digest, Vol 237, Issue 4 (Eddie Martin) > 3. Re: Long term museum specimen storage (John Kiernan) > 4. Re: Detergent in heating antigen retrieval (Gudrun Lang) > 5. p17 mice brain sections (Alonso Mart?nez Canabal) (Amos Brooks) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 20 Sep 2023 13:24:55 -0600 > From: Alonso Mart?nez Canabal > To: Histonet > Subject: [Histonet] Detergent in heating antigen retrieval > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Dear histoneters, > I have performed heating antigen retrieval with citrate buffer > pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, > anyone has any experience or knowledge if it is better with or without the > detergent? > Thank you! > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > > > ------------------------------ > > Message: 2 > Date: Wed, 20 Sep 2023 21:24:14 -0400 > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Histonet Digest, Vol 237, Issue 4 > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Thought on alternative for Sudan Black. I don't use this stain...an > alternative is an Oil Red O stain. Oil Red O is done on frozen > sections...but you can also deparaffinize FFPE sections to water, and then > perform your Oil Red O stain. > > I hope this helps. > > Very Respectfully, > Eddie Martin > > Eddie Martin, HTL, QIHC > The National Institutes of Health > Bone Marrow Service > 10 Center Drive > Building 10, Room 2C360 > Bethesda, MD 20892 > Office: 301-594-2054 > > >> On Thu, Aug 10, 2023 at 12:59?PM >> wrote: >> >> Send Histonet mailing list submissions to >> histonet at lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request at lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner at lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> Today's Topics: >> >> 1. Happy Hump Day Histopeeps!! Summer is Almost OVER!!! Here are >> some exciting new opportunities for you and your friends! >> (relia1 at earthlink.net) >> 2. Sudan Black B (Betsy Molinari) >> >> >> >> ---------- Forwarded message ---------- >> From: >> To: >> Cc: >> Bcc: >> Date: Wed, 9 Aug 2023 14:13:39 -0400 >> Subject: [Histonet] Happy Hump Day Histopeeps!! Summer is Almost OVER!!! >> Here are some exciting new opportunities for you and your friends! >> Happy Hump Day Histopeeps!! >> >> It is hard to believe the summer is almost over. The kids are going back >> to school and Labor Day is just a few weeks away! >> >> A few more weeks after that ?Halloween; Then Thanksgiving, Christmas, and >> New Year?s Day!! >> >> ? Looking for a new job in the Fall? >> >> ? Planning a job change after the holidays? >> >> ? Considering making a move in 2024? >> >> Let?s Get The Ball Rolling!!! >> >> Histonetters! >> >> **Are you a histo tech looking for something better? >> >> **Are you a new or recent graduate of a histology school? >> >> **Are you a traveler transitioning into a permanent position? >> >> **Are you a Histotech looking to advance into leadership? >> >> **Are you a Supervisor looking to advance to lab management? >> >> Let?s Get The Ball Rolling!!! >> >> All you have to do is contact me at relia1 at earthlink.net >> or call/text me at 407-353-5070 or call me >> at >> 866-607-3542 my office is open M-F 7am-8pm EST or by appointment. >> >> >> >> If you know of anyone else who might be interested in subscribing to >> RELIA?s Histology Careers Bulletin, please feel free to pass this along to >> them. >> >> I have exciting opportunities in: >> >> ? Leadership >> >> ? Tech Support >> >> ? Mohs >> >> ? GI >> >> ? Derm >> >> ? Clinical >> >> ? AP >> >> ? Research >> >> These Opportunities are located in: >> >> ? Florida ? Brand New Lab! >> >> ? Washington >> >> ? South Carolina >> >> ? Arizona >> >> ? California >> >> ? Massachusetts >> >> ? Wisconsin >> >> ? Tennessee >> >> ? Georgia >> >> ? Alabama >> >> I invite you to join my group on Facebook: >> >> www.facebook.com/groups/histotechnologists >> >> >> Have a Great Week! >> >> >> >> >> >> Thanks-Pam >> >> Right Time, Right Place, Right Move with RELIA! >> >> Providing excellent service exclusively to the Histology Community! >> >> Thank You! >> >> Pam M. Barker >> >> Pam Barker >> >> President/Senior Recruiting Specialist-Histology >> >> RELIA Solutions >> >> Specialists in Allied Healthcare Recruiting >> >> 5717 Red Bug Lake Road #330 >> >> Winter Springs, FL 32708-4969 >> >> Phone: (407)657-2027 >> >> Cell: (407)353-5070 >> >> FAX: (407)678-2788 >> >> Toll free: (866)60RELIA or (866)607-3542 >> >> E-mail: relia1 at earthlink.net >> >> https://www.facebook.com/RELIASolutionsforhistologyprofessionals >> >> www.linkedin.com/in/reliasolutions >> >> >> I invite you to join my group on Facebook: >> >> www.facebook.com/groups/histotechnologists >> >> >> Follow my hashtags to make your day great and your career greater! >> >> #ilovemyhistopeeps >> >> #jobs4myhistopeeps >> >> #histologyiscool >> >> #histologyjobs >> >> #histologycareers >> >> #histology >> >> >> >> >> >> >> >> >> >> >> >> ---------- Forwarded message ---------- >> From: Betsy Molinari >> To: Histonet >> Cc: >> Bcc: >> Date: Thu, 10 Aug 2023 14:57:31 +0000 >> Subject: [Histonet] Sudan Black B >> Hi, >> I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. >> The biopsy is in a paraffin block. They are looking to better report and >> understand the IHC. I am totally unfamiliar with this stain. I did some >> reading but have been unable to find a protocol for paraffin sections. I >> found a reference to Sheehan & Hrapchak (1973) but unfortunately I don't >> have that edition. Any ideas would be greatly appreciated . >> >> Betsy Molinari HT (ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 1101 Bates St. >> Houston, Texas 77030 >> 832-355-6524 >> >> Betsy Molinari, HT (ASCP) >> Sr. Histology Research Technician >> CV Pathology Research >> >> The Texas Heart Institute (r) >> 6770 Bertner Avenue, MC 1-283 >> Houston, TX 77030 >> >> Office: 832-355-6524 | Fax: 832-355-6812 >> Email: BMolinari at texasheart.org >> texasheart.org | texasheartmedical.org< >> https://www.texasheartmedical.org/> | facebook< >> https://www.facebook.com/Texas.Heart.Institute> | twitter< >> https://twitter.com/Texas_Heart> >> >> CONFIDENTIALITY NOTICE: This email and attachments contain information >> that may be confidential or privileged. If you are not the intended >> recipient, notify the sender at once and delete this message completely >> from your information system. Further use, disclosure, or copying of >> information contained in this email is not authorized, and any such action >> should not be construed as a waiver of privilege or other confidentiality >> protections. >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 3 > Date: Thu, 21 Sep 2023 05:20:13 +0000 > From: John Kiernan > To: Histonet , Rhonda McCormick > > Subject: Re: [Histonet] Long term museum specimen storage > Message-ID: > > > Content-Type: text/plain; charset="iso-8859-1" > > I don't know anything about "Jore's fixative" or the rationale of using a very hypertonic unbuffered 4% formaldehyde with magnesium, sodium, chloride and sulphate ions. If brown stuff is now bleeding out of your museum specimens, Jore Juice evidently isn't a good preservative. > > According to Chapter 26 in the late Charles Culling's excellent book (3rd edn 1974; ISBN: 0407729011) the fixative/preservative for a museum specimen is optimized to preserve colour, which is the red or reddish-brown of haemoglobin and myoglobin. This usually is achieved with Kaiserling's fluid, which contains formalin, potassium acetate and also potassium nitrate (1.5% w/v) as an oxidant. Another approach involves treating specimens with carbon monoxide to convert all haemoglobin etc to a red carboxy derivative. > > If your museum specimens have already lost all their meaningful colours, a neutral buffered aqueous formaldehyde may be the best that you can provide to preserve the sizes and shapes. 70% alcohol will cause some shrinkage, and it may not be as easy to seal this solvent into a museum container as a watery diluted formalin. > > John Kiernan > = = = > ________________________________ > From: Rhonda McCormick via Histonet > Sent: September 20, 2023 11:39 AM > To: Histonet > Subject: [Histonet] Long term museum specimen storage > > Hi All, > I am looking to replace the fixative for veterinary specimens that have been preserved as "museum specimens". They are kept in jars in a glass case outside our lab, however, some of the fixative is starting to turn brown (and we've pulled a few jars that have some slight cracks in them). > The specimens are currently in Jore's Fixative: 100 mL Distilled water > 10 mL 40% Formaldehyde2 g Magnesium Sulfate2 g Sodium Sulfate1 g Sodium Chloride > Preserving specimens is new to me. I've never heard of Jore's fixative before and I'm wondering if I could get some advice, please? Do these specimens need to be replaced with the same solution? Could we rinse the specimen and replace the solution with 70% Alcohol? OR would 10% NBF be better to store the specimens in (or something al together different)? We have a varying display of specimens - anywhere from a small porcine optic nerve to a large equine granulosa cell tumor. Realizing it may be different based on the size of the specimen, approximately how often should the solution be changed ? > Thank you so much! Any help or insight is much appreciated. > Rhonda McCormickRhonda McCormick BS, HT (ASCP)cm > Histology Diagnostic Lab Supervisor > > College of Veterinary Medicine > Texas A&M University > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 4 > Date: Thu, 21 Sep 2023 13:28:58 +0200 > From: "Gudrun Lang" > To: 'Alonso Mart?nez Canabal' > Cc: > Subject: Re: [Histonet] Detergent in heating antigen retrieval > Message-ID: <001001d9ec7e$d2102c50$763084f0$@gmx.at> > Content-Type: text/plain; charset="utf-8" > > Hi, > I think the answer is as so often: it depends. Detergens solves membranes partly and leads to a higher permeabilization of the tissue. Some antigens may take advantage of that, some may not need it. > Higher permeability is good for detection with high molecular complexes. > > Gudrun Lang > > -----Urspr?ngliche Nachricht----- > Von: Alonso Mart?nez Canabal via Histonet [mailto:histonet at lists.utsouthwestern.edu] > Gesendet: Mittwoch, 20. September 2023 21:25 > An: Histonet > Betreff: [Histonet] Detergent in heating antigen retrieval > > Dear histoneters, > I have performed heating antigen retrieval with citrate buffer > pH 6 with 0.05% tween-20, however I have seem recipes with no detergent, > anyone has any experience or knowledge if it is better with or without the > detergent? > Thank you! > > -- > Dr. Alonso Mart?nez Canabal PhD > Profesor Asociado "C" > Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM > Investigador Nacional "I" > 56224833 > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Thu, 21 Sep 2023 12:40:40 -0400 > From: Amos Brooks > To: "histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] p17 mice brain sections (Alonso Mart?nez Canabal) > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > Hi, > The thicker the section the more likely it will be to fall off. Frozen > sections already love to fall off slides. You should cut them a lot > thinner. 4 to 10 um should be the thickness, especially for frozens. > If you want very thick sections as you describe, you would be better > off cutting them and transferring them directly to an 8 well plate with PBS > and doing the IHC as a floating section. > > Cheers, > Amos Brooks > > >> >> Message: 1 >> Date: Tue, 19 Sep 2023 19:07:04 -0600 >> From: Alonso Mart?nez Canabal >> To: Histonet >> Subject: [Histonet] p17 mice brain sections >> Message-ID: >> > wDugsDPPX5YTkxXUaipmwctzjcAZwf_d4A at mail.gmail.com> >> Content-Type: text/plain; charset="UTF-8" >> >> Hello, >> Have a great afternoon. I have done HAR with citrate buffer (pH 6.0) >> with tween, basically all my professional life of some 15 years. Several >> publications, using mice brain sections 40-50 microns thickness from >> cryostat (30% sucrose). Today I tried to do some p17 brain sections and the >> sections did not only fell off, but were completely destroyed. >> That never happened to me, I am wonder if anyone can have any idea of >> what happened? >> >> Thank you so much >> >> -- >> Dr. Alonso Mart?nez Canabal PhD >> Profesor Asociado "C" >> Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM >> Investigador Nacional "I" >> 56224833 >> > > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 238, Issue 10 > ***************************************** From Lesley.Bechtold at jax.org Fri Sep 22 08:47:42 2023 From: Lesley.Bechtold at jax.org (Lesley Bechtold) Date: Fri, 22 Sep 2023 13:47:42 +0000 Subject: [Histonet] Short Benchmarking Survey In-Reply-To: References: Message-ID: Dear Histonetters, Periodically, the Histology Service at The Jackson Laboratory carries out benchmarking surveys to see where we fit with other Histology Labs. If you are also interested in benchmarking, please answer the questions below and return them to me directly. I will send the de-identified results to everyone who answers our survey. If you have any questions, please feel free to contact me. Many thanks in advance. Lesley Bechtold lesley.bechtold at jax.org Associate Director, Histopathology Sciences The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 www.jax.org 1. Name of Institution 2. Type of institution (i.e., hospital, university, pharmaceutical company, etc.)? 3. Faculty Size? 4. Number of groups or individuals using the Histology Service 5. Do you charge for your services? 6. Labor rate (per hour)? 7. Labor rate calculation method (i.e., Blended labor rate of materials + labor? Accounting for unbillable time or using a flat average? Do you charge for labor only or do you charge for materials only?) 8. Number of employees (full-time and part-time)? 9. Paraffin blocks - number per year? 10. Paraffin blocks - price per block? 11. H&Es - number per year? 12. H&Es - price per slide? 13. Do you do IHC? Manual or automated? 14. Average turn-around time - from tissue to slide (hours, days, weeks)? 15. Annual total expenditures? 16. Annual total recovery if fees are charged? --- The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From umbellas at hotmail.com Tue Sep 26 05:44:49 2023 From: umbellas at hotmail.com (Gerard Spoelstra) Date: Tue, 26 Sep 2023 18:44:49 +0800 Subject: [Histonet] Stored frozen fatty muscle tissue Message-ID: Hi Histonetters I?ve been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower the temperature. They are only small biopsies, but are heavily marbled. Any ideas? Gerard Spoelstra From relia1 at earthlink.net Tue Sep 26 10:07:01 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 26 Sep 2023 11:07:01 -0400 Subject: [Histonet] BRAND NEW LABS IN PA OH and IL RELIA EXCLUSIVES!! Message-ID: <004101d9f08b$1b7da600$5278f200$@earthlink.net> Histopeeps, I have some exciting opportunities with brand new and recently opened labs. This is YOUR chance to do more and learn more. These are permanent full time positions. My clients offer compensation packages that RIVAL the current offerings in TRAVEL! For more information: Private message me, email me at relia1 at earthlink.net or call/text me at 407-353-5070 Have a great day! Thanks- Pam Barker #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From jmacdonald at mtsac.edu Tue Sep 26 11:32:32 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 26 Sep 2023 16:32:32 +0000 Subject: [Histonet] Stored frozen fatty muscle tissue In-Reply-To: References: Message-ID: Are you allowing them to come up to the temperature of the cryostat before cutting? -----Original Message----- From: Gerard Spoelstra via Histonet Sent: Tuesday, September 26, 2023 3:45 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Stored frozen fatty muscle tissue EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hi Histonetters I've been trying to cut frozen fatty muscle tissue from 2018/2019 stored at -80C. I managed to cut some sections from the same tissue on fresh frozen tissue in 2018. I suspect that storage may contribute to the difficulty to cut this. I have yet touse dry ice to further lower the temperature. They are only small biopsies, but are heavily marbled. Any ideas? Gerard Spoelstra _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jsagasser at gandhigi.com Tue Sep 26 13:04:59 2023 From: jsagasser at gandhigi.com (Jacque Sagasser) Date: Tue, 26 Sep 2023 18:04:59 +0000 Subject: [Histonet] Electronic requisitions Message-ID: Hello Does anyone use electronic requisitions? If so, would you mind describing your system for staying compliant with CLIA? Is a paper requisition sent in addition to the electronic requisition? If not, how does that work with accessioning and matching the specimens and patient information? We currently use a paper system and the requisitions are filed and kept for 10 years. We would like to move to an electronic system, but I am not sure how to implement it, while staying compliant and not complicating the process. We do not have a bar code system set up. I could scan the paper requestions into the patient file, but that would be more work than just continuing to file them. I am also running out of room to file the requisitions though, so I am trying to explore my options. Thank you in advance for your input. ?Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagasser at gandhigi.com 937-795-1099 phone 937-519-1321 fax From tbraud at holyredeemer.com Thu Sep 28 08:39:36 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 28 Sep 2023 13:39:36 +0000 Subject: [Histonet] [EXTERNAL] Histonet Digest, Vol 238, Issue 13 In-Reply-To: References: Message-ID: We have something that is a bit of a hybrid that we call a "dummy" requisition. A person "orders" a "test" for pathology or cytology in the hospital information system. The form they fill out has all of the information that would appear on a paper requisition. When completed, it prints to a printer in Pathology and is matched to the specimens in the department (this could print to where the specimens are collected). The printed copy becomes the requisition. The pathology dummy test is autoresulted as "see pathology report". The actual accessioning of the specimen into the LIS creates the true pathology test with result(report) pending. Advantages: One can make required fields (such as clinical history) so that they must be filled out, which captures information required by CAP. Cons: A pain when something needs to be corrected. We usually have them cancel the order and submit another order. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 -----Original Message----- Today's Topics: 1. Electronic requisitions (Jacque Sagasser) From: Jacque Sagasser Subject: [Histonet] Electronic requisitions Hello Does anyone use electronic requisitions? If so, would you mind describing your system for staying compliant with CLIA? Is a paper requisition sent in addition to the electronic requisition? If not, how does that work with accessioning and matching the specimens and patient information? We currently use a paper system and the requisitions are filed and kept for 10 years. We would like to move to an electronic system, but I am not sure how to implement it, while staying compliant and not complicating the process. We do not have a bar code system set up. I could scan the paper requestions into the patient file, but that would be more work than just continuing to file them. I am also running out of room to file the requisitions though, so I am trying to explore my options. Thank you in advance for your input. Jacque R. Sagasser, HT (ASCP)cm Gandhi GI Pathology, LLC 999 Brubaker Drive Suite 1 Kettering OH 45429 jsagasser at gandhigi.com 937-795-1099 phone 937-519-1321 fax From Lesley.Bechtold at jax.org Thu Sep 28 09:21:32 2023 From: Lesley.Bechtold at jax.org (Lesley Bechtold) Date: Thu, 28 Sep 2023 14:21:32 +0000 Subject: [Histonet] Please fill out our benchmarking survey Message-ID: Dear Histonetters, Periodically, the Histology Service at The Jackson Laboratory carries out benchmarking surveys to see where we fit with other Histology Labs. If you are also interested in benchmarking, please answer the questions below and return them to me directly. I will send out the de-identified results to everyone who answers our survey. You may not be able to fill out all the questions but any data is helpful. The survey will close down on Friday, Oct. 6th. If you have any questions, please feel free to contact me. Feel free to forward this survey onto anyone you think would benefit from this data. Many thanks in advance. Lesley Bechtold lesley.bechtold at jax.org Associate Director, Histopathology Sciences The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 www.jax.org 1. Name of Institution 2. Type of institution (i.e., hospital, university, pharmaceutical company, etc.)? 3. Faculty Size? 4. Number of groups or individuals using the Histology Service 5. Do you charge for your services? 6. Labor rate (per hour)? 7. Labor rate calculation method (i.e., Blended labor rate of materials + labor? Accounting for unbillable time or using a flat average? Do you charge for labor only or do you charge for materials only?) 8. Number of employees (full-time and part-time)? 9. Paraffin blocks - number per year? 10. Paraffin blocks - price per block? 11. H&Es - number per year? 12. H&Es - price per slide? 13. Do you do IHC? Manual or automated? 14. Average turn-around time - from tissue to slide (hours, days, weeks)? 15. Annual total expenditures? 16. Annual total recovery if fees are charged? --- The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible. From relia1 at earthlink.net Fri Sep 29 11:42:54 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 29 Sep 2023 12:42:54 -0400 Subject: [Histonet] RELIA Hot Job Alert! Fantastic opportunity in Houston, TX including a 10K sign on bonus and RELO! Message-ID: <018801d9f2f4$00091ec0$001b5c40$@earthlink.net> Hello Histopeeps, How are you? I hope you are having a wonderful day and are looking forward to a nice weekend! I have a exciting opportunity in Houston,TEXAS! Work on the leading edge of Cancer Diagnostics! Use your IHC and histology skills to contribute, grow and learn! I am working with one of my BEST clients in Texas that is in need of a histopeep like you! My client is offering an exciting opportunity that is a permanent full-time position with excellent compensation and benefits, including a 10K sign on bonus and relocation assistance. Histopeep, would you be interested in this position? OR Do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So, if you think you or someone you know might be interested please contact me. I can be reached at toll free at 866-607-3542, or cell/text407-353-5070 or email me at relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas, please let me know. I am getting calls for histology professionals at all levels practically everywhere on almost DAILY basis!! Join your fellow histopeeps for fun and information at my Facebook group just for you!! www.facebook.com/groups/histotechnologists Have a Great Weekend! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology