From dlh3878 at gmail.com Wed Nov 1 14:43:19 2023 From: dlh3878 at gmail.com (Donielle Hunter) Date: Wed, 1 Nov 2023 15:43:19 -0400 Subject: [Histonet] SOS In need of IHC controls Message-ID: Hey everyone!! I am the lab manager for a private owned path lab for a GI office Ohio. We are in desperate need of positive controls for HSV I and II and are hoping to start doing DOG-1, c kit, and CD34 on site instead of sending out to a satellite lab. Would anyone have control tissue to spare?? Majority of our controls are from our patient tissue and would be willing to donate what controls we can from our tissue that is at its 10 year point. I used this platform last time we needed controls for our HSV and a generous donor gave us enough to last 3 years! I appreciate any help and thanks in advance DL Hunter From jaylundgren at gmail.com Thu Nov 2 09:58:42 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 2 Nov 2023 09:58:42 -0500 Subject: [Histonet] Microcredentials for Histology In-Reply-To: References: Message-ID: What Jennifer said. Just what kind of jumped-up osteopath college are you people running up there? Shocking. You think they're looking for "microcredentials" at M.D. Anderson or Brigham and Women's? You're not going to help the shortage or the field by lowering standards. Just kill a bunch of patients. You want to attract registered/licensed HT/Ls? PAY MORE MONEY! You call yourself a Medical School (since 2018), act like one. Please show this email to your boss. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) Virus-free.www.avast.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Fri, Oct 27, 2023 at 3:39?PM Mac Donald, Jennifer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > With route 2 the applicants must have at least one year of full-time > acceptable experience working histology with experience in microtomy, > embedding, fixation, staining, and lab operations. They will be required > to provide proof of the employment/experience to the ASCP with their > transcripts. > > -----Original Message----- > From: Kara, Phillip via Histonet > Sent: Friday, October 27, 2023 9:34 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Microcredentials for Histology > > EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > Good morning everyone. > > I recently started a position with the University of North Texas Health > Science Center as their Histology Research Associate. During a quick > meeting with my director he mentioned that he was interested in possibly > setting up a 'microcredentialing' program for Histology since our lab is > now up and running. > Pretty much from what he was telling me it would be similar to when we > were in high school taking those computer classes where we would get the > Microsoft certifications showing we knew how to use Word, Excel, > Powerpoint, ect. > My hope would be to try setting it up in such a way that students when > finished would have a better understanding of what we do in Histology but > also have the basics to possibly sit (depending on how long out program > would last) for the HT exam using the route 2 option ["Both require prior > education in the histology field, either through attending a histology > program, or acquiring > laboratory experience in the field."] My question for the field is 2 fold. > > 1. Is this something that you think could be a second path for people > to get into the field of Histology due to our nation wide shortage? We deal > with med students, pre med, medical research students, and starting a > nursing program here next year. > 2. If this could work what do you think I should focus on? My initial > thoughts were of course sectioning, processing, maybe even some staining. > But what else? > > Interested to hear everyone's thoughts on the topic. > > [ > https://mail.google.com/mail/u/0?ui=2&ik=8e07797374&attid=0.1&permmsgid=msg-f:1778656250049141922&th=18af0e6f89b51ca2&view=fimg&fur=ip&sz=s0-l75-ft&attbid=ANGjdJ8LWmuH5_BzU-y7vwwBOWcW_Ue6DO0UFBd0pTuQUq3tVH-HDPbJqzQxDFkDYKhFdT1mINf6UvGbJXAVg6WgX_U5TGZ-Dbo_vlWhmEqc8vfEpdnm-RoiZo2RO3c&disp=emb > ] > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w: http://www.unthsc.edu/corelabs > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From paula at excaliburpathology.com Thu Nov 2 10:29:24 2023 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 2 Nov 2023 15:29:24 +0000 (UTC) Subject: [Histonet] Microcredentials for Histology In-Reply-To: References: Message-ID: <1649297098.1904635.1698938964109@mail.yahoo.com> Here! Here! I sure would not want the pilot flying a plane I was on to have micro credentials. Like, hey I learned how to raise the landing gear this week.? Standards should be raised, not doing just enough to get by. In histology, all steps in totality create the final product. I still think blocks and slides should be sent in as part of the registry, as we did in the past. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, November 2, 2023 at 10:14:07 AM CDT, Jay Lundgren via Histonet wrote: What Jennifer said. Just what kind of jumped-up osteopath college are you people running up there?? Shocking. You think they're looking for "microcredentials" at M.D. Anderson or Brigham and Women's? You're not going to help the shortage or the field by lowering standards. Just kill a bunch of patients. You want to attract registered/licensed HT/Ls?? PAY MORE MONEY! You call yourself a Medical School (since 2018), act like one. Please show this email to your boss. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) Virus-free.www.avast.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2> On Fri, Oct 27, 2023 at 3:39?PM Mac Donald, Jennifer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > With route 2 the applicants must have at least one year of full-time > acceptable experience working histology with experience in microtomy, > embedding, fixation, staining, and lab operations.? They will be required > to provide proof of the employment/experience to the ASCP with their > transcripts. > > -----Original Message----- > From: Kara, Phillip via Histonet > Sent: Friday, October 27, 2023 9:34 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Microcredentials for Histology > >? EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > Good morning everyone. > > I recently started a position with the University of North Texas Health > Science Center as their Histology Research Associate. During a quick > meeting with my director he mentioned that he was interested in possibly > setting up a 'microcredentialing' program for Histology since our lab is > now up and running. > Pretty much from what he was telling me it would be similar to when we > were in high school taking those computer classes where we would get the > Microsoft certifications showing we knew how to use Word, Excel, > Powerpoint, ect. > My hope would be to try setting it up in such a way that students when > finished would have a better understanding of what we do in Histology but > also have the basics to possibly sit (depending on how long out program > would last) for the HT exam using the route 2 option ["Both require prior > education in the histology field, either through attending a histology > program, or acquiring > laboratory experience in the field."] My question for the field is 2 fold. > >? 1.? Is this something that you think could be a second path for people > to get into the field of Histology due to our nation wide shortage? We deal > with med students, pre med, medical research students, and starting a > nursing program here next year. >? 2.? If this could work what do you think I should focus on? My initial > thoughts were of course sectioning, processing, maybe even some staining. > But what else? > > Interested to hear everyone's thoughts on the topic. > > [ > https://mail.google.com/mail/u/0?ui=2&ik=8e07797374&attid=0.1&permmsgid=msg-f:1778656250049141922&th=18af0e6f89b51ca2&view=fimg&fur=ip&sz=s0-l75-ft&attbid=ANGjdJ8LWmuH5_BzU-y7vwwBOWcW_Ue6DO0UFBd0pTuQUq3tVH-HDPbJqzQxDFkDYKhFdT1mINf6UvGbJXAVg6WgX_U5TGZ-Dbo_vlWhmEqc8vfEpdnm-RoiZo2RO3c&disp=emb > ] > > Phillip Kara, HTL | Senior Research Associate > > University of North Texas Health Science Center > > Division of Research and Innovation > > a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107 > > p: 918-281-9060 > > w: http://www.unthsc.edu/corelabs > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Mon Nov 6 15:30:38 2023 From: cforster at umn.edu (Colleen Forster) Date: Mon, 6 Nov 2023 15:30:38 -0600 Subject: [Histonet] LEAD HISTOTECHNOLOGIST OPPORTUNITY In-Reply-To: References: Message-ID: I would love too but unable to move the whole family! Colleen Forster On Fri, Oct 27, 2023 at 11:20?AM Stephanie L. Thompson via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Come to beautiful New Hampshire, the best beaches, Great White Mountains, > no income or state taxes, quality education, friendly people. Relocation > Costs included. > > Quality is in our DNA -- is it in yours? > > You are a superhero when it comes to patient specimens. You've got > problem-solving instincts, a passion for patient care, and the drive to > keep things running smoothly. You're also looking for great benefits, the > support of an all-star team, and an opportunity to grow your career. > > Join our front line of #HealthcareHeroes! Our mission is to advance the > health and wellbeing of our communities as a leader in clinical laboratory > solutions. > > Location: Exeter, NH > Days: Monday - Friday > 9:00 PM - 5:30 AM > Full-time: Benefit Eligible > > * All you need is: > * > * Bachelor's Degree in the field of Laboratory Science or equivalent > under the 1988 Clinical Laboratory Improvement Amendments (CLIA) guidelines > of high complexity testing. > * State licensure, if applicable. > * Certified or eligible for Board of Certification (BOC) by the > American Society of Clinical Pathologists (ASCP) > * Completion of a Histology program accredited by the National > Accrediting Agency for Clinical Laboratory Sciences (NAACLS) or minimum of > one (1) year experience as a full-time Histology Technician Trainee and > competent in the areas of fixation, processing, embedding, microtomy, > grossing, special stains, immunohistochemistry, and lab operations. > * > * Company: > * Sonic Anatomic Pathology > * > * We'll give you: > * Appreciation for your work > * A feeling of satisfaction that you've helped people > * Opportunity to grow in your profession > * Free lab services for you and your dependents > * Work-life balance, including Paid Time Off and Paid Holidays > * Competitive benefits including medical, dental, and vision > insurance > * Help saving for retirement, with a 401(k) plus a company match > * A sense of belonging - we're a community! > * > > This message contains privileged and confidential information intended > only for the use of the addressee named above. If you are not the intended > recipient of this message you must not disseminate, copy, or take any > action in reliance on it. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From relia1 at earthlink.net Tue Nov 7 12:27:41 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 7 Nov 2023 13:27:41 -0500 Subject: [Histonet] ICYMI Why leaving may be your best option for Career Advancement and Some NEW and Exciting Opportunities. Message-ID: <005c01da11a8$1896b9c0$49c42d40$@earthlink.net> Hi Histonetters!?I hope you are having a fantastic day! If you are someone who wants to move up in your career?NOW?is the perfect time to shine! Why? The staffing shortages open opportunities for?YOU?to ? Learn?MORE ? Do?MORE, ? Get?MORE Experience?AND?Opportunity to further your career if not where you are now then it?IS?out there?somewhere else! I can speak from experience that my clients are giving motivated histotechs opportunities to be hired in at higher level positions based on potential not just immediate need!?(That panic hiring is for travelers) I was surfing the internet and came across this article and had to share it with you: Why Leaving may be the best option for career advancement. https://ivyexec.com/career-advice/2018/leaving-career-advancement-best-opti on/ ?Think about it for a second? ?????????Your next opportunity might be just around the corner. ?????????If you are looking for a position right now, please contact me right away.? ?????????We can talk about my current positions?OR?about a customized search on your behalf.? ?????????If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too.? ?????????To do that, send me an email atmailto:relia1 at earthlink.net??or call me on my cell at?407-353-5070. My Clients are looking for histopeeps to fill roles in: ????????TEACHING!! ????????Leadership ????????IHC ????????Mohs ????????Diagnostics These amazing opportunities with some of the best labs in the USA are located in: ????????Florida ????????Virginia ????????California ????????Georgia ????????Alabama ????????Texas ????????Arizona ????????Illinois ????????Pennsylvania ????????South Carolina ????????Connecticut All of these are full time permanent positions offering the same or BETTER compensation than Travel positions!?? Have doubts about this let me show you how! My clients are offering relocation and or sign on bonuses up to 20K!! And new opportunities coming in?on a daily basis! Most of these are?RELIA?Exclusives!??That?s right you will only see them?HERE! I really appreciate you taking time to read my e-mail Thanks Again! Incidentally, if you have another area in mind let me know so I can be on the lookout. Thanks-Pam I would like to invite you to join my group on Facebook:? ? http://www.facebook.com/groups/histotechnologists It?s a great place to learn, laugh and share with your fellow histopeeps! Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! I hope you have a wonderful week. Remember if you refer someone I place your will earn a referral bonus! Thank You! ?Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIASolutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:?????? (407)353-5070 FAX:?????? (407)678-2788 E-mail:?mailto:relia1 at earthlink.net??? https://www.facebook.com/RELIASolutionsforhistologyprofessionals http://www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell:? (407)353-5070 FAX:???? (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net?? https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From nmargaryan88 at gmail.com Tue Nov 7 15:57:51 2023 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Tue, 7 Nov 2023 15:57:51 -0600 Subject: [Histonet] Microtome block holder Message-ID: Hello, We are thinking to process the dog stifle joints. For that reason we will need large cassette/slides (3x2) and more importantly to find a large block holder for the Leica Microtome. Any advice and suggestions where to purchase all large parts would be appreciated. Thanks in advance, Naira From cforster at umn.edu Tue Nov 7 16:01:12 2023 From: cforster at umn.edu (Colleen Forster) Date: Tue, 7 Nov 2023 16:01:12 -0600 Subject: [Histonet] Microtome block holder In-Reply-To: References: Message-ID: Naira, Contact Lee Dickey with Ted Pella. They carry the whole line of large block adapters for the Leica microtomes. lee_dickey at tedpella.com I have this set up and it works great. Colleen Forster HT(ASCP)QIHC On Tue, Nov 7, 2023 at 3:58?PM Naira Margaryan via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > We are thinking to process the dog stifle joints. For that reason we will > need large cassette/slides (3x2) and more importantly to find a large block > holder for the Leica Microtome. > > Any advice and suggestions where to purchase all large parts would be > appreciated. > > Thanks in advance, > Naira > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From nmargaryan88 at gmail.com Tue Nov 7 16:02:28 2023 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Tue, 7 Nov 2023 16:02:28 -0600 Subject: [Histonet] Microtome block holder In-Reply-To: References: Message-ID: Awesome, thank you so much Colleen, for such quick response, Naira On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster wrote: > Naira, > > Contact Lee Dickey with Ted Pella. They carry the whole line of large > block adapters for the Leica microtomes. > > lee_dickey at tedpella.com > > I have this set up and it works great. > > Colleen Forster HT(ASCP)QIHC > > On Tue, Nov 7, 2023 at 3:58?PM Naira Margaryan via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hello, >> >> We are thinking to process the dog stifle joints. For that reason we will >> need large cassette/slides (3x2) and more importantly to find a large >> block >> holder for the Leica Microtome. >> >> Any advice and suggestions where to purchase all large parts would be >> appreciated. >> >> Thanks in advance, >> Naira >> > _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >> > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > > Minneapolis, MN 55455 > > 612-626-1930 > > > > > > > From cforster at umn.edu Tue Nov 7 16:03:21 2023 From: cforster at umn.edu (Colleen Forster) Date: Tue, 7 Nov 2023 16:03:21 -0600 Subject: [Histonet] Microtome block holder In-Reply-To: References: Message-ID: You are very welcome! Colleen On Tue, Nov 7, 2023 at 4:02?PM Naira Margaryan wrote: > Awesome, thank you so much Colleen, for such quick response, > Naira > > On Tue, Nov 7, 2023 at 4:01 PM Colleen Forster wrote: > >> Naira, >> >> Contact Lee Dickey with Ted Pella. They carry the whole line of large >> block adapters for the Leica microtomes. >> >> lee_dickey at tedpella.com >> >> I have this set up and it works great. >> >> Colleen Forster HT(ASCP)QIHC >> >> On Tue, Nov 7, 2023 at 3:58?PM Naira Margaryan via Histonet < >> histonet at lists.utsouthwestern.edu> wrote: >> >>> Hello, >>> >>> We are thinking to process the dog stifle joints. For that reason we will >>> need large cassette/slides (3x2) and more importantly to find a large >>> block >>> holder for the Leica Microtome. >>> >>> Any advice and suggestions where to purchase all large parts would be >>> appreciated. >>> >>> Thanks in advance, >>> Naira >>> >> _______________________________________________ >>> Histonet mailing list >>> Histonet at lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>> >> >> -- >> Colleen Forster HT(ASCP)QIHC >> BLS Histology and IHC Laboratory >> Jackson Hall, Room 2-155 >> 321 Church St. SE >> >> Minneapolis, MN 55455 >> >> 612-626-1930 >> >> >> >> >> >> >> -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From mbireir at yahoo.com Tue Nov 7 18:59:09 2023 From: mbireir at yahoo.com (MANAHIL EL BIREIR) Date: Wed, 8 Nov 2023 04:59:09 +0400 Subject: [Histonet] Orientation for new Staff References: <7257A0DF-78AB-4001-9285-EB2754998AAC.ref@yahoo.com> Message-ID: <7257A0DF-78AB-4001-9285-EB2754998AAC@yahoo.com> Hi everyone, Could any share intradepartmental orientation or task?s checklist for new arrival staff. Thanks, Manahil From tcampbell at fgamd.com Wed Nov 8 13:43:54 2023 From: tcampbell at fgamd.com (Campbell, Tasha) Date: Wed, 8 Nov 2023 19:43:54 +0000 Subject: [Histonet] Microwave Processing Message-ID: <9ee459d3934142b2b1382b11527a808f@fgamd.com> Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 From bcooper at chla.usc.edu Wed Nov 8 14:36:27 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 8 Nov 2023 20:36:27 +0000 Subject: [Histonet] Microwave Processing In-Reply-To: <9ee459d3934142b2b1382b11527a808f@fgamd.com> References: <9ee459d3934142b2b1382b11527a808f@fgamd.com> Message-ID: <052df977594b4979ae7ca13123b85075@chla.usc.edu> Hi Tasha, Maybe look into getting a line conditioner for your tissue processors. Your tissue processor vendor may have some suggestions. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Campbell, Tasha via Histonet Sent: Wednesday, November 8, 2023 11:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1iXiR7DgxMp8b_BUwsOCVLYh5BrZ18oRqXPXSrsq6yyJTaeSklH-M9e_nxo0H1edrZXY8yW2gB8uBPQFlrkycg81-6fiZfp9m4ew3W1GIWPzMJxjQlg5CqwTVlRq0Cyjnm_YOVGfYZOiimFv4mFXsvQ4s6Z5mi6VGMQ9l9EV181iFfWy_EdrhCytI8lcrPR5T3NjeUxeI0fA9cFJZ7LWxqU--onyetKWST0IMK268d0AjEQ7OSLKvdYXVmYyVJAzxfipn9ViDmsCqXJRxf4uITFghZAI2yDQKiuWScym6vAcxOar9XYPwUrdH57HihsGPipJmQxk7mxyMmOsxjl-Hao_xLjG2Vk5YaQwnVbDu_3Wghi1tH8eSyr9-aN3yczudiSqKuNpUfLXBO5tnIwNkVhpnspiPX0-LrFtTxl5TTZGGeAUdxUxy3O4osa6kJqifCnXi6k8ZX46q32cw2YH4vw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From tcampbell at fgamd.com Wed Nov 8 14:39:48 2023 From: tcampbell at fgamd.com (Campbell, Tasha) Date: Wed, 8 Nov 2023 20:39:48 +0000 Subject: [Histonet] Microwave Processing In-Reply-To: <052df977594b4979ae7ca13123b85075@chla.usc.edu> References: <9ee459d3934142b2b1382b11527a808f@fgamd.com>, <052df977594b4979ae7ca13123b85075@chla.usc.edu> Message-ID: <1FCFAA6C-A31C-4A73-B938-AE7BE4CBC791@fgamd.com> What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > ?Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > Department of Pathology and Laboratory Medicine > Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcooper at chla.usc.edu > > -----Original Message----- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://url.emailprotection.link/?bXyKyZCpvAJAuWZpEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Swkd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0PATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdLT_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qflag8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnLis5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH911zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K547D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4~ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From bcooper at chla.usc.edu Wed Nov 8 14:41:48 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 8 Nov 2023 20:41:48 +0000 Subject: [Histonet] Microwave Processing In-Reply-To: <1FCFAA6C-A31C-4A73-B938-AE7BE4CBC791@fgamd.com> References: <9ee459d3934142b2b1382b11527a808f@fgamd.com>, <052df977594b4979ae7ca13123b85075@chla.usc.edu> <1FCFAA6C-A31C-4A73-B938-AE7BE4CBC791@fgamd.com> Message-ID: <5f65c3ccc7f54d1aab7185d94ed7b867@chla.usc.edu> They may make a combo UPS/line conditioner. That would be ideal. Line conditioners stabilize and protect against power spikes/surges. Thanks, Brian -----Original Message----- From: Campbell, Tasha Sent: Wednesday, November 8, 2023 12:40 PM To: Cooper, Brian Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Microwave Processing (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: tcampbell at fgamd.com. What does a line conditioner do? The company was bought out recently and basically has no employees so I have no one to help me. Tasha Campbell Sent from my iPhone > On Nov 8, 2023, at 3:36 PM, Cooper, Brian wrote: > > Hi Tasha, > > Maybe look into getting a line conditioner for your tissue processors. Your tissue processor vendor may have some suggestions. > > Thanks, > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of > Pathology and Laboratory Medicine Children's Hospital Los Angeles > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > Ph: 323.361.3357 > bcooper at chla.usc.edu > > -----Original Message----- > From: Campbell, Tasha via Histonet > Sent: Wednesday, November 8, 2023 11:44 AM > To: histonet at lists.utsouthwestern.edu > Subject: [Histonet] Microwave Processing (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. > > > Tasha Campbell, B.S., HTL (ASCP) > Frederick Gastroenterology Associates > 7109 Guilford Dr. Suite 300 > Frederick, MD 21704 > 301-695-6800 ext. 144 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://secure-web.cisco.com/1xOy41N6xvg0UteDOIpjvHf_CApfMcmUGNNj458SM > -CGccqMtGH-FueUgDT2-PuUzi4tvd_GkWrTORVTZWS5BZJgE72NR2xJJTk01-cOaT-nzZM > tUbO3ZfdoGwdKFXuKi0Qq7wY6p0enhF_HVv0kxEW-8mnROFvKHXVmVK3ntIMO6OhYNRZVw > X-VTPtEydHHpARUr1BSXsHA3MtHNTBt4HykE0OXOK8asWk8RQW_yKcYjWxSEEwH_vD1ocX > Up48AzfKg8xNdL56OW30ndSRD5hmp0Gpp-mLx0fN5_ET34tkBsdiHLyQg0kk3pJOLACTze > dmSiOPdFtx_ZRJ-ytfdE1V08WztA5BHIJhSrt44_FhbVF5KKndReERS2HveVBcsPKFebUL > FXvEP72PvfysukCulHG3OpPnMFY_IXE2fkNA8_EQhQGsOmCVyg7Y9AICeRTtXwI9DytSpM > f7XCTujLDQ/https%3A%2F%2Furl.emailprotection.link%2F%3FbXyKyZCpvAJAuWZ > pEARN9oWjGEc0NHCYoC4VTztcJcf6GYOHMwDA04MoeflfYb1xPjKWMnZkaOCqeOtNoZ4Sw > kd-VyGHnZDhhsZd6fJhf9axt9VVVZ50GIwj4DnibSEV8tf2M7CLjuHYF9MkMPooCQ4Mt0P > ATuuXc0T64axJSX2gX7INjxTgJxGB_LdNp_wf2pRolRjTD6LT6OcXK7vrZgrIgSwnTljdL > T_anURFohI0k-BfXgqgcHF6KIYIK23zWTmIg3tG0Sbiybk0fQ_cSufokcCBEdzJ7X8qfla > g8Qfau0FsEpgdw5sDb49u1tC5rIVFbE7XPwklXgZA1K2hCImmIcuRZyO_TcmopBwK598tM > 1u4L5MHzmYR6AXF4ClgS7r2ofMK0RsiZIcnPq2daWo7Pfh6fs7eV1avQ-x0wojgsvi_XnL > is5CwLvu6oaRjdbCzHjtxYS2nfdzJYUEqHClxcA7Twh0DlyJ3vEf8iqCEdWX5p0nW9EvH9 > 11zINBjt0lUDflMBMXT4tkDigoynpB7nJH3RZDt9yBFTZ4HQSoYKUJcatXnLiZ2qw-tIY1 > GRcxdriVyv_XEubDtu2IsBa-S69JOaJXnnuwrK2EcEgHbXlTs-ZunA8_KfVoyBf6bj3K54 > 7D0LehZYi4ZTn3_l8jMqlPyw8-4eJ1SiU_iyIh4%7E > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From duraine at bcm.edu Wed Nov 8 16:47:30 2023 From: duraine at bcm.edu (Duraine, Lita R) Date: Wed, 8 Nov 2023 22:47:30 +0000 Subject: [Histonet] Microwave Processing In-Reply-To: <9ee459d3934142b2b1382b11527a808f@fgamd.com> References: <9ee459d3934142b2b1382b11527a808f@fgamd.com> Message-ID: Hello all, I use microwave processing exclusively. First of all, are you making sure that each reagent is completely extracted before the next reagent is administered? I know the tissue cannot be completely dry, but all residue reagent should be siphoned off before the next one goes in. This is especially important when dehydrating the tissue with ethanol, acetone, or polypropylene reagents. I usually do at least 3 resin changes if not 4. Curing the resin is another issue I've seen. Even though protocol says to cure for three days, I have found curing for a week is better. Especially important to cure the resin blocks longer in humid, rainy, or cold environments. Another issue is the diamond knives. Make sure that your clearance angle is correct and the water in the boat meets the knife edge, if you are doing TEM. Check those things and happy sectioning. Lita Sent from my Verizon, Samsung Galaxy smartphone -------- Original message -------- From: "Campbell, Tasha via Histonet" Date: 11/8/23 1:44 PM (GMT-06:00) To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Microwave Processing Is there anyone that is super familiar with microwave processing? I really need some help. I am having trouble with my tissue and I think it's the processing. I don't know what else it would be. We moved to a new building so I don't know if the power could be stronger and its changing the way it processes or what. I have tried messing with the program times and temps but I cannot figure it out. My blocks keep having what looks like knife lines but it's not the microtomes. The only change has been moving into a brand new building. Any help would be appreciated! Thanks. Tasha Campbell, B.S., HTL (ASCP) Frederick Gastroenterology Associates 7109 Guilford Dr. Suite 300 Frederick, MD 21704 301-695-6800 ext. 144 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=DwICAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Z-Quiwr_oQfGYLNUELwQ1A&m=V2zfrROQEcGKlRUW4kujQYF33S2YA8Y2zc14D8heDFEQhQs5URz_aaD3SVRB-BEY&s=Abfh6qOZ3XnmvUsraeSWPUfFcn1Ow8IUH6TreF8Ivnk&e= From psjayalakshmy at gmail.com Thu Nov 9 10:32:46 2023 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Thu, 9 Nov 2023 22:02:46 +0530 Subject: [Histonet] Faded H&E tissue section Message-ID: Hello, I would like to know how to effectively restain a faded H&E tissue section. The color becomes dull when re stained. Somebody please advise. Prof. Jayalakshmy. P S From amosbrooks at gmail.com Thu Nov 9 13:33:39 2023 From: amosbrooks at gmail.com (Amos Brooks) Date: Thu, 9 Nov 2023 14:33:39 -0500 Subject: [Histonet] Faded H&E tissue section Message-ID: Hi, Don't decolonize. Soak the coverslip off and let it sit in xylene to make absolutely sure all the mounting media is off then simply re-stain it. If there is any mounting media left on it will interfere with the staining. Unless there's something wrong with either the section or the reagents, it should work just fine. Amos Brooks Message: 6 Date: Thu, 9 Nov 2023 22:02:46 +0530 From: "jayalakshmy p.s" To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Faded H&E tissue section Message-ID: Content-Type: text/plain; charset="UTF-8" Hello, I would like to know how to effectively restain a faded H&E tissue section. The color becomes dull when re stained. Somebody please advise. Prof. Jayalakshmy. P S From afhenwood at outlook.com Thu Nov 9 19:28:50 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Fri, 10 Nov 2023 01:28:50 +0000 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: You could treat the decoverslipped section in 1% periodic acid (same as used in the PAS technique) for 30 minutes prior to H&E staining. This might improve the H&E staining. I believe Lee Luna suggested this but for the life of me, I can?t find the reference! Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: jayalakshmy p.s via Histonet Sent: Friday, 10 November 2023 3:43 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Faded H&E tissue section Hello, I would like to know how to effectively restain a faded H&E tissue section. The color becomes dull when re stained. Somebody please advise. Prof. Jayalakshmy. P S _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psjayalakshmy at gmail.com Thu Nov 9 20:11:19 2023 From: psjayalakshmy at gmail.com (jayalakshmy p.s) Date: Fri, 10 Nov 2023 07:41:19 +0530 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: Thanks, I'll check it out. On Fri, Nov 10, 2023, 6:58 AM Tony Henwood wrote: > You could treat the decoverslipped section in 1% periodic acid (same as > used in the PAS technique) for 30 minutes prior to H&E staining. This > might improve the H&E staining. > > > > I believe Lee Luna suggested this but for the life of me, I can?t find the > reference! > > > > Regards, > > > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > > Principal Scientist, the Children?s Hospital at Westmead (Retired) > > Adjunct Fellow, School of Medicine, University of Western Sydney. > > > > *From: *jayalakshmy p.s via Histonet > *Sent: *Friday, 10 November 2023 3:43 AM > *To: *histonet at lists.utsouthwestern.edu > *Subject: *[Histonet] Faded H&E tissue section > > > > Hello, > I would like to know how to effectively restain a faded H&E tissue section. > The color becomes dull when re stained. Somebody please advise. > Prof. Jayalakshmy. P S > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From akemiat3377 at gmail.com Thu Nov 9 20:30:20 2023 From: akemiat3377 at gmail.com (Akemi Allison) Date: Thu, 9 Nov 2023 18:30:20 -0800 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn?t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E?s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat?s tips which he didn?t publish, but I shared them in Frieda?s 4th Ed. Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet wrote: > > ?Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H&E staining. This >> might improve the H&E staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can?t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children?s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *histonet at lists.utsouthwestern.edu >> *Subject: *[Histonet] Faded H&E tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H&E tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afhenwood at outlook.com Fri Nov 10 03:49:08 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Fri, 10 Nov 2023 09:49:08 +0000 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: I thought he may have reported this in Histologic. This trick certainly works. You can notice the nuclear clarity on GIT and to a lesser extent skin biopsies stained with PAS. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Akemi Allison Sent: Friday, 10 November 2023 1:30 PM To: jayalakshmy p.s Cc: Tony Henwood; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Faded H&E tissue section Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn?t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E?s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat?s tips which he didn?t publish, but I shared them in Frieda?s 4th Ed. Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet wrote: > > ?Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H&E staining. This >> might improve the H&E staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can?t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children?s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *histonet at lists.utsouthwestern.edu >> *Subject: *[Histonet] Faded H&E tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H&E tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Fri Nov 10 12:40:30 2023 From: rsrichmond at gmail.com (Bob Richmond) Date: Fri, 10 Nov 2023 13:40:30 -0500 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: > > > I recall learning the technique of using 1% periodic acid to brighten up > dismal hematoxylin staining, when I was a resident at Johns Hopkins in > 1970. I don't know where the histochemist I learned it from got it, though Bob Richmond Maryville TN From akemiat3377 at gmail.com Fri Nov 10 18:36:23 2023 From: akemiat3377 at gmail.com (Akemi Allison) Date: Fri, 10 Nov 2023 16:36:23 -0800 Subject: [Histonet] =?utf-8?q?John_Caggiano=E2=80=99s_passing?= Message-ID: <60B4329C-CD1D-42C9-B04C-8BFC5D163F4B@gmail.com> Dear HistoPeeps: I have some sad news to share with the histology/pathology community. Joe Caggiano informed me Monday that his brother John passed away this last Sunday night. John Caggiano, founder, President and CEO of Poly Scientific R & D Corp, died quietly in his sleep after a long illness. John and Poly Scientific were early and longtime supporters of NSH, (National Society for Histotechnologists). He was always supportive, generous, and it was a pleasure to spend time with him. John was a great friend and he will be greatly missed. RIP John. Regards, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone From POWELL_SA at mercer.edu Fri Nov 10 19:38:15 2023 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Sat, 11 Nov 2023 01:38:15 +0000 Subject: [Histonet] =?windows-1252?q?John_Caggiano=92s_passing?= In-Reply-To: <60B4329C-CD1D-42C9-B04C-8BFC5D163F4B@gmail.com> References: <60B4329C-CD1D-42C9-B04C-8BFC5D163F4B@gmail.com> Message-ID: Oh my goodness, my heart is so heavy. I love John and Joe and all the Caggiano family. They were so helpful with all the state societies and supporteted us consistently through our early years and on into the later ones as well. They are such a part of our profession and I will miss John so much. So sorry Joe and Caggiano family. Thanks for letting us know. Shirley Powell Now "Retired" HTL ________________________________ From: Akemi Allison via Histonet Sent: Friday, November 10, 2023 4:36 PM To: Histonet Subject: [Histonet] John Caggiano?s passing Dear HistoPeeps: I have some sad news to share with the histology/pathology community. Joe Caggiano informed me Monday that his brother John passed away this last Sunday night. John Caggiano, founder, President and CEO of Poly Scientific R & D Corp, died quietly in his sleep after a long illness. John and Poly Scientific were early and longtime supporters of NSH, (National Society for Histotechnologists). He was always supportive, generous, and it was a pleasure to spend time with him. John was a great friend and he will be greatly missed. RIP John. Regards, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7Cdcafb0b7b3b74456adb908dbe24e5454%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C638352598280996691%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0F1reuYTxvo6vCHShUGD9MEnZO1w5NUl4FbAdZbevmE%3D&reserved=0 From jkiernan at uwo.ca Sat Nov 11 00:42:43 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 11 Nov 2023 06:42:43 +0000 Subject: [Histonet] Faded H&E tissue section In-Reply-To: References: Message-ID: I found the Histo-Logic archive on one of Sakura's web pages. Lee Luna was the editor for several years. Nothing there before about 1980 periodic acid for re-working H&E. The archive is huge and many contributions look good. See https://www.sakuraus.com/getattachment/103878b9-9854-469d-8203-9d9b1c45d850/761 but bear in mind that this is from a company. It's not a peer-reviewed scientific journal. John Kiernan. = = = ________________________________ From: Tony Henwood via Histonet Sent: November 10, 2023 4:49 AM To: Akemi Allison ; jayalakshmy p.s Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Faded H&E tissue section I thought he may have reported this in Histologic. This trick certainly works. You can notice the nuclear clarity on GIT and to a lesser extent skin biopsies stained with PAS. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Akemi Allison Sent: Friday, 10 November 2023 1:30 PM To: jayalakshmy p.s Cc: Tony Henwood; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Faded H&E tissue section Hi Tony: I actually gave that tip to Lee back in 1979 when he came to OHSU to give a seminar to the Oregon Histology Society. I found out that when I was doing GMA and the pathologists didn?t like Gills Hematoxylin. They loved my PAS on GMA so I tried using 1% Periodic Acid before using Harris Hematoxylin for H&E?s on GMA and it turned out beautifully. Guess he shared my tip with the rest of our society. He and I became closer se friends and we shared several tips, including his Movat?s tips which he didn?t publish, but I shared them in Frieda?s 4th Ed. Best, Akemi Allison-Tacha, BS, HT/HTL (ASCP) Sent from my iPhone > On Nov 9, 2023, at 6:11 PM, jayalakshmy p.s via Histonet wrote: > > ?Thanks, I'll check it out. > >> On Fri, Nov 10, 2023, 6:58 AM Tony Henwood wrote: >> >> You could treat the decoverslipped section in 1% periodic acid (same as >> used in the PAS technique) for 30 minutes prior to H&E staining. This >> might improve the H&E staining. >> >> >> >> I believe Lee Luna suggested this but for the life of me, I can?t find the >> reference! >> >> >> >> Regards, >> >> >> >> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) >> >> Principal Scientist, the Children?s Hospital at Westmead (Retired) >> >> Adjunct Fellow, School of Medicine, University of Western Sydney. >> >> >> >> *From: *jayalakshmy p.s via Histonet >> *Sent: *Friday, 10 November 2023 3:43 AM >> *To: *histonet at lists.utsouthwestern.edu >> *Subject: *[Histonet] Faded H&E tissue section >> >> >> >> Hello, >> I would like to know how to effectively restain a faded H&E tissue section. >> The color becomes dull when re stained. Somebody please advise. >> Prof. Jayalakshmy. P S >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Mon Nov 13 09:04:11 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 13 Nov 2023 15:04:11 +0000 Subject: [Histonet] John Caggiano's passing Message-ID: <3a32c9f59fc5489497f96d1c9fba605b@holyredeemer.com> I just returned from a week off to see the notice of John Caggiano's passing. My deepest condolences to his family and friends. His unwavering commitment and support to the field of Histotechnology can't be understated. RIP, you will be missed. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion ***Please Note: Dec. 1, 2023, Redeemer Health will be updating its email domain from @holyredeemer . com to @redeemerhealth . org. Please alert your IT/cybersecurity team to ensure our new email domain is safe-listed.*** This email, and any files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. If you are not the intended recipient, please telephone or email the sender and delete this message and any attachment from your system; you must not copy or disclose the contents of this message or any attachment to any other person. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Redeemer Health. Redeemer Health may monitor email traffic data. From relia1 at earthlink.net Mon Nov 13 09:47:44 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Mon, 13 Nov 2023 10:47:44 -0500 Subject: [Histonet] Histology Educator needed for growing program in Florida! Can you help? Message-ID: <00a501da1648$befa9300$3cefb900$@earthlink.net> Hi Histonetters! I hope this is the beginning of a fantastic week for you! I have an educator position in Florida, and I need your help. This is a full time permanent salaried position, and my client is offering an excellent compensation package and an amazing team to work with. ASCP certification is required to obtain Florida licensure which is required for the position. The help I need is this: Do you know of anyone who might be interested in this position? OR Would you be interested in this position? If you are a histo tech who loves histology that wants to get off the bench and inspire and educate the next generation, we need to talk. These opportunities don't come along very often so even if you are just mildly curious please contact me. Just shoot me an email to relia1 at earthlink.net or you can reach me on my cell/text at 407-353-5070. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From generashun_net at yahoo.com Fri Nov 17 12:07:57 2023 From: generashun_net at yahoo.com (Robert Herrera) Date: Fri, 17 Nov 2023 18:07:57 +0000 (UTC) Subject: [Histonet] Microwave Tissue Processors References: <1895963580.4077163.1700244477279.ref@mail.yahoo.com> Message-ID: <1895963580.4077163.1700244477279@mail.yahoo.com> Quick question; are there any facilities utilizing ?microwave tissue processors?? If so, can I get the name and number of the company that is servicing the microwave. We have three that need servicing. Thank YouRobert Sent from Yahoo Mail for iPhone From Donna.Willis at BSWHealth.org Fri Nov 17 12:28:33 2023 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Fri, 17 Nov 2023 18:28:33 +0000 Subject: [Histonet] Microwave Tissue Processors In-Reply-To: <1895963580.4077163.1700244477279@mail.yahoo.com> References: <1895963580.4077163.1700244477279.ref@mail.yahoo.com> <1895963580.4077163.1700244477279@mail.yahoo.com> Message-ID: Who is the manufacturer of the unit that you are using? Donna Willis HT/HTL (ASCP) Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave. Dallas, Texas 75246 214-820-2465 office 214-725-6184 cell -----Original Message----- From: Robert Herrera via Histonet Sent: Friday, November 17, 2023 12:08 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Microwave Tissue Processors CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Quick question; are there any facilities utilizing ?microwave tissue processors?? If so, can I get the name and number of the company that is servicing the microwave. We have three that need servicing. Thank YouRobert Sent from Yahoo Mail for iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!C7apPYp7EPf3krE_yL_y5gYnktTiCAQj2fCokelY5_7zXWxqVoVZn6QItyHncVwJVEb4xdXBIbDzt3AizepZJjAHvVt_egKH$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From amorissette at liberty.edu Sat Nov 18 14:09:45 2023 From: amorissette at liberty.edu (Morissette, Amie) Date: Sat, 18 Nov 2023 20:09:45 +0000 Subject: [Histonet] Identifying the Ideal Mobility Scooter: A Comprehensive Guide Message-ID: Choosing a suitable mobility scooter is a significant decision for individuals with mobility issues, as it greatly impacts their independence and lifestyle. This guide aims to aid you in thoroughly examining the array of mobility scooters available, ensuring that you select one that best fits your specific requirements and enhances your mobility. Determining Your Mobility Needs The initial step in finding the right mobility scooter involves a careful assessment of your individual mobility needs. Consider the typical environments you'll navigate, your daily routines, and any specific physical challenges you face. If you primarily encounter smooth, paved pathways, a compact scooter may be sufficient. In contrast, for navigating more challenging, uneven terrains, a scooter with a stronger build and longer battery life would be more suitable. Categories of Mobility Scooters * Compact/Travel Scooters: These are ideal for active individuals or those with limited storage space, as they are lightweight and can be easily disassembled for transport. * 3-Wheel Scooters: Known for better maneuverability and more leg space, these scooters are great for indoor use and smooth outdoor surfaces. * 4-Wheel Scooters: Providing increased stability, these scooters are better suited for outdoor use, particularly on uneven grounds. * Heavy-Duty Scooters: Designed for larger individuals, these models have higher weight capacities and are built for enhanced durability and performance. Key Features to Evaluate * Battery Duration: Important for users who need to operate their scooter for extended periods without frequent recharging. * Comfort Aspects: Adjustable seating, armrests, and steering controls are crucial for comfort, especially for long-term use. * Weight Tolerance: The scooter should be capable of comfortably supporting your weight. * Portability Features: For those who travel often, a scooter that is foldable or easy to disassemble can be very useful. * Wheel and Suspension Design: Essential for a smooth ride across various terrains. * Safety Measures: Features like anti-tip wheels, lights, and reflective elements are important for ensuring safe operation. Budget Considerations and Insurance Mobility scooters come in various price ranges, influenced by their features and construction. It's important to find a balance between your needs and your financial capacity. Additionally, it?s worth checking with your insurance provider, as some plans, like Medicare Part B, may cover part of the cost if the scooter is medically necessary. Selecting a Reputable Supplier It's crucial to buy from a trustworthy supplier. Look for suppliers that offer strong customer support and maintenance services. A recommended place to start is www.everestmobility.com, which offers a broad selection of mobility scooters along with expert advice. Test Rides and Customer Reviews If possible, test drive a variety of models to better understand their comfort and handling. Additionally, consulting customer reviews and seeking advice from existing users can provide further insights. Conclusion Selecting the right mobility scooter is a decision that profoundly affects your daily mobility and quality of life. By thoroughly understanding your needs, exploring different models, and considering essential features in light of your budget, you can make an informed choice. For a wide selection and expert guidance, resources like www.everestmobility.com are invaluable. In summary, the process of choosing the best mobility scooter involves a personalized approach, detailed research, and expert consultation. With the right scooter, you can significantly improve your independence and daily experience. From Zhengmei.Mao at uth.tmc.edu Mon Nov 20 15:09:07 2023 From: Zhengmei.Mao at uth.tmc.edu (Mao, Zhengmei) Date: Mon, 20 Nov 2023 21:09:07 +0000 Subject: [Histonet] RA Lamb microwriter cassette printer Message-ID: Hello, I have a Thermo Scientific RA Lamb microwriter E22.01 MWI cassette printer with Shandon Barcode Writer software. I'm not able to find a manual or any info on how to use the printer/software. Could anyone knowledgeable with this system kindly help? Many thanks, Mei M UTHealth From acanabal at ciencias.unam.mx Wed Nov 22 16:29:47 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Wed, 22 Nov 2023 16:29:47 -0600 Subject: [Histonet] DAB freezing Message-ID: Hi everyone, I am doing free-floating immunohistochemistry with brain slices. That is only the context. The issue is that I always prepare my DAB (Hydrochloride sigma powder) aliquot it and store it in -20?C. But recently, once I thaw it the DAB quality decreases a lot, to almost not have any signal (I am used to almost black neurons with NiSO4). But all the protocols that I have found say that is okay to make aliquots and store them like that. Is there a stybilizer or something that I could use to make the stock solution last? or my only option is to prepare it always immediately before use? Thank you! -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 From carl.hobbs at kcl.ac.uk Fri Nov 24 02:12:02 2023 From: carl.hobbs at kcl.ac.uk (Carl Hobbs) Date: Fri, 24 Nov 2023 08:12:02 +0000 Subject: [Histonet] DAB freezing (Alonso Mart?nez Canabal) Message-ID: I do manual IHC-DAB regularly DAB shouldn't "go off" after freezing unless you have somehow oxidised it during mixing/aliquoting. It would then show as a dark brown aliquot I state this because I still make up my own DAB from dry powder ( have been doing for > 20yrs) SIgma D5637 I dissolve, aliquot and freeze, as you do. An aliquot may be stored at -20C for 2yrs before I defrost/use it If I run out of 50microL aliquots I will defrost a 1ml aliquot, re-aliquot into 50 microL ( when I am doing IHC on a few slides, I make up a small working DAB vol.) Perhaps your H2O2 ( substrate) stock has "gone off"? That will give you a weaker/negative DAB result NB: left-over working DAB solution can be stored at 4C for at least 7 days and still be perfectly usable ( NOT re-usable) If I have only 5-10 slides I will do the DAB step in the humidity chamber, applying DAB as I would antibody (only into the hydrophobic pen-ringed section) Good luck and...please let us know the answer when you solve your problem Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 From tbraud at holyredeemer.com Fri Nov 24 14:37:09 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 24 Nov 2023 20:37:09 +0000 Subject: [Histonet] Weak DAB from frozen Message-ID: I agree with Carl Hobbs: "Perhaps your H2O2 ( substrate) stock has 'gone off'? That will give you a weaker/negative DAB result." If your frozen DAB aliquots keep the same color, I would be very suspect of the Hydrogen Peroxide. It can become unstable very quickly. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Today's Topics: 1. Re: DAB freezing (Alonso Mart?nez Canabal) (Carl Hobbs) ---------------------------------------------------------------------- Message: 1 From: Carl Hobbs Re: [Histonet] DAB freezing (Alonso Mart?nez Canabal) I do manual IHC-DAB regularly DAB shouldn't "go off" after freezing unless you have somehow oxidised it during mixing/aliquoting. It would then show as a dark brown aliquot I state this because I still make up my own DAB from dry powder ( have been doing for > 20yrs) SIgma D5637 I dissolve, aliquot and freeze, as you do. An aliquot may be stored at -20C for 2yrs before I defrost/use it If I run out of 50microL aliquots I will defrost a 1ml aliquot, re-aliquot into 50 microL ( when I am doing IHC on a few slides, I make up a small working DAB vol.) Perhaps your H2O2 ( substrate) stock has "gone off"? That will give you a weaker/negative DAB result NB: left-over working DAB solution can be stored at 4C for at least 7 days and still be perfectly usable ( NOT re-usable) If I have only 5-10 slides I will do the DAB step in the humidity chamber, applying DAB as I would antibody (only into the hydrophobic pen-ringed section) Good luck and...please let us know the answer when you solve your problem Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 ***Please Note: Dec. 1, 2023, Redeemer Health will be updating its email domain from @holyredeemer . com to @redeemerhealth . org. Please alert your IT/cybersecurity team to ensure our new email domain is safe-listed.*** This email, and any files transmitted with it, are confidential and intended solely for the use of the individual or entity to whom they are addressed. The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. If you are not the intended recipient, please telephone or email the sender and delete this message and any attachment from your system; you must not copy or disclose the contents of this message or any attachment to any other person. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Redeemer Health. Redeemer Health may monitor email traffic data. From umbellas at hotmail.com Mon Nov 27 01:22:02 2023 From: umbellas at hotmail.com (Gerard Spoelstra) Date: Mon, 27 Nov 2023 15:22:02 +0800 Subject: [Histonet] Frozen marbled muscle failing to cut Message-ID: Hi I resolved the problem with my earlier post from the 26th of September by cutting the muscle at a lower temperature -18C. The muscle has previously been embedded in OCT immersed in Isopentane and the into liquid nitrogen and stored in a freezer at -80C. The tissue is Wagyu muscle some of which is highly marbled. The higher marble tissue will not cut. It just leaves hole where the tissue should be. I suspect that the OCT has failed to infiltrate the more highly marbled muscle. This sometimes occurs with brain in paraffin embedded sections. After reprocessing the problem is resolved. I don?t know if it?s possible to solve this in this case. Any Ideas? Thanks Gerard Spoelstra From Valerie.Hannen at parrishmed.com Wed Nov 29 14:40:32 2023 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Wed, 29 Nov 2023 20:40:32 +0000 Subject: [Histonet] CAP Cytopathology checklist question Message-ID: Hello Everyone, I am hoping you can help me. I am very confused by one of the questions in our CAP Cytopathology question. How are you handling/ answering this question in the NON-GYNCOLOGIC CYTOPATHLOOGY Section? The question is CYP.07666, Unsatisfactory Specimens- Non-gynecologic CytoPathology: It states " The Laboratory follows defined criteria for identification and reporting of unsatisfactory non-gynecologic specimens, as applicable". It shows that I must write a policy for this question. Our reports do state the reason for being deemed a "unsatisfactory" specoimen. Thank you in advance!! Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From LNormington at uwhealth.org Thu Nov 30 08:07:51 2023 From: LNormington at uwhealth.org (Normington, Lacy) Date: Thu, 30 Nov 2023 14:07:51 +0000 Subject: [Histonet] CAP Cytopathology checklist question In-Reply-To: References: Message-ID: Hello Valerie, Here is the policy we created to meet this question. Standardized criteria for specimen adequacy are applied where available. This includes the Paris System for Reporting Urinary Cytology, the Bethesda System for reporting Anal/Rectal Cytology, and the Bethesda System for reporting Thyroid Cytopathology. The additional criteria below apply to all other non-gynecological specimen types. 1. A specimen is unsatisfactory if there's an absence of abnormal cells AND any of the following criteria are met: * The complete absence of cellular material. * Insufficient cellular material present to verify the source of the specimen (i.e. mesothelial cells in a serous fluid, alveolar macrophages in a BAL, etc.). * The absence of lymphocytes in a lymph node FNA * The presence of benign cellular material on an FNA that cannot explain a clinically suspicious mass or lesion. 2. All unsatisfactory non-gynecologic specimens include a description of the specimen's limitations (i.e. low cellularity). References 1. Rosenthal D, Wojcik EM, Kurtycz DF. The Paris system for reporting urinary cytology. Switzerland: Springer International Publishing; 2016. 2. Nayar R, Wilbur DC. The Bethesda system for reporting cervical cytology - definitions, criteria, and explanatory notes. 3rd ed. Switzerland: Springer International Publishing; 2015. 3. Ali SZ, Cibas ES. The Bethesda System for Reporting Thyroid Cytopathology. New York, NY: Springer; 2010. -----Original Message----- From: Hannen, Valerie via Histonet Sent: Wednesday, November 29, 2023 2:41 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP Cytopathology checklist question WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello Everyone, I am hoping you can help me. I am very confused by one of the questions in our CAP Cytopathology question. How are you handling/ answering this question in the NON-GYNCOLOGIC CYTOPATHLOOGY Section? The question is CYP.07666, Unsatisfactory Specimens- Non-gynecologic CytoPathology: It states " The Laboratory follows defined criteria for identification and reporting of unsatisfactory non-gynecologic specimens, as applicable". It shows that I must write a policy for this question. Our reports do state the reason for being deemed a "unsatisfactory" specoimen. Thank you in advance!! Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com http://www.parrishmed.com/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Valerie.Hannen at parrishmed.com Thu Nov 30 09:39:02 2023 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 30 Nov 2023 15:39:02 +0000 Subject: [Histonet] CAP Cytopathology checklist question In-Reply-To: References: Message-ID: Good Morning Lacy, Thank you for the sharing your policy, it will be very helpful in creating my own. -----Original Message----- From: Normington, Lacy Sent: Thursday, November 30, 2023 9:08 AM To: Hannen, Valerie ; Histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] RE: CAP Cytopathology checklist question ***WARNING*** This message came from an external source. Please do not click links or open attachments if unexpected or unusual due to high security risk. Actual Sender Address: LNormington at uwhealth.org Begin Original Message: Hello Valerie, Here is the policy we created to meet this question. Standardized criteria for specimen adequacy are applied where available. This includes the Paris System for Reporting Urinary Cytology, the Bethesda System for reporting Anal/Rectal Cytology, and the Bethesda System for reporting Thyroid Cytopathology. The additional criteria below apply to all other non-gynecological specimen types. 1. A specimen is unsatisfactory if there's an absence of abnormal cells AND any of the following criteria are met: * The complete absence of cellular material. * Insufficient cellular material present to verify the source of the specimen (i.e. mesothelial cells in a serous fluid, alveolar macrophages in a BAL, etc.). * The absence of lymphocytes in a lymph node FNA * The presence of benign cellular material on an FNA that cannot explain a clinically suspicious mass or lesion. 2. All unsatisfactory non-gynecologic specimens include a description of the specimen's limitations (i.e. low cellularity). References 1. Rosenthal D, Wojcik EM, Kurtycz DF. The Paris system for reporting urinary cytology. Switzerland: Springer International Publishing; 2016. 2. Nayar R, Wilbur DC. The Bethesda system for reporting cervical cytology - definitions, criteria, and explanatory notes. 3rd ed. Switzerland: Springer International Publishing; 2015. 3. Ali SZ, Cibas ES. The Bethesda System for Reporting Thyroid Cytopathology. New York, NY: Springer; 2010. -----Original Message----- From: Hannen, Valerie via Histonet Sent: Wednesday, November 29, 2023 2:41 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] CAP Cytopathology checklist question WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Hello Everyone, I am hoping you can help me. I am very confused by one of the questions in our CAP Cytopathology question. How are you handling/ answering this question in the NON-GYNCOLOGIC CYTOPATHLOOGY Section? The question is CYP.07666, Unsatisfactory Specimens- Non-gynecologic CytoPathology: It states " The Laboratory follows defined criteria for identification and reporting of unsatisfactory non-gynecologic specimens, as applicable". It shows that I must write a policy for this question. Our reports do state the reason for being deemed a "unsatisfactory" specoimen. Thank you in advance!! Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com http://www.parrishmed.com/ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CyM303mrztY!epXyHa2swgUEum3FfKIgyfHrR3vfS5AdYqZj8uYr3HdXg5J0i2EDxNjNWQjcuhVunPIW7J2oYsBetLIrQ1pJ4E_DhcQpPA$ From relia1 at earthlink.net Thu Nov 30 12:05:26 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 30 Nov 2023 13:05:26 -0500 Subject: [Histonet] ICYMI: Epic Holiday Party FAIL! And Some exciting new job opportunities for now or 2024! Your choice! Message-ID: <001e01da23b7$dad65d80$90831880$@earthlink.net> Hi Histopeeps, I hope this will be an amazing week for you! It's Holiday party time. I found this article online and it made me laugh so I had to share it. In 3 words: Epic Party Fail! "I will confront you by Wednesday of this week" https://www.askamanager.org/2021/12/i-will-confront-you-by-wednesday-of-this -week-2.html Do you have a funny Holiday party story? If so, I would love to hear/read it!! I know looking at a new job opportunity is the furthest thing from your mind this week but always on my mind wink wink... Histopeeps, I do have some exciting new opportunities and my clients are willing to wait for you until after the holidays or after your contract ends. All of these are full time permanent positions, and my clients offer excellent compensation packages including in most cases a sign on bonus and or relocation assistance. And all of them have fantastic teams ready to welcome you!! My current openings: IL - Chicago Mohs Tech Full time excellent opportunity! AZ - Tucson Mohs Tech Full time excellent opportunity! TX - Dallas Mohs Tech Full time excellent opportunity! GA - Brunswick HT/HTL GA - Atlanta HT/HTL VA - Charlottesville HT/HTL and 15K sign on bonus! TX - Houston IHC specialist Cancer Research. FL - Ft. Myers Quality Specialist. Yes, It's December and clients are STILL calling with exciting new opportunities! Pam, if you are looking for something new locally or in another area and you don't see it listed here, PLEASE let me know. I got several of these positions in the past few hours!!! I have new positions coming all of the time and would love to be on the lookout for YOU! Remember, refer someone and I place them you earn a referral bonus! Help Your Friends and Help Me and Get Rewarded! If you or someone you know would like more information, please contact me. I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Happy Holidays!! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From mtighe at trudeauinstitute.org Thu Nov 30 14:23:39 2023 From: mtighe at trudeauinstitute.org (Mike Tighe) Date: Thu, 30 Nov 2023 20:23:39 +0000 Subject: [Histonet] Control slides and/or blocks Message-ID: I am curious to know if there is any interest in positive/negative control tissue blocks or slides (FFPE) with any number of pathogens. We are an infectious disease research center and we are always looking to expand our contract research services. We work mostly with mouse models but from time to time we also work with hamster and ferret tissues. Pathogens include SARS-COV2, Influenza virus, Zika virus, Dengue virus, as well as mycobacterium tuberculosis and many other bacterial pathogens. Thanks in advance for any thoughts on the subject! Mike From tgenade at protonmail.com Thu Nov 30 20:46:16 2023 From: tgenade at protonmail.com (Tyrone Genade) Date: Fri, 01 Dec 2023 02:46:16 +0000 Subject: [Histonet] O.C.T. what MW PVA and PEG? Message-ID: Hello, I'm using a cryoprotection protocol that involves 3-stage cryoprotection of 15% glucose O/N, then 30% + 50% OCT O/N and then finally O/N in OCT. Compared to previous protocols this works very well -- even when cutting through eyes and lenses (which had previously given a lot of grief). My issue is that preparing the 30% + 50% OCT is a schlep. The OCT puts up a lot of resistance against mixing with the 60% sucrose. It would be much simpler if I could prepare 30% sucrose with powdered PVA and PEG. Does anyone know what MW polymers of PVA and PEG to use and what concentrations to approximate commercial (Scigen Tissue-Plus) OCT? Thanks Tyrone Genade Ph.D. Quillen College of Medicine ETSU Johnson City, TN From jkiernan at uwo.ca Thu Nov 30 23:47:35 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Fri, 1 Dec 2023 05:47:35 +0000 Subject: [Histonet] O.C.T. what MW PVA and PEG? In-Reply-To: References: Message-ID: Your method makes no sense! It looks like something informally passed along among students and technicians who have never read a book. Cryoprotection means preventing formation of ice crystals or, as it's usually done, minimizing their size. Sucrose is a cryoprotectant; the higher the concentration the better, but strong solutions penetrate the tissue slowly. For fixed specimens dissolve the sucrose in water. (For unfixed, use an isotonic phosphate buffer, but that's only if a day or so at 4C is OK for your needs. Very fast freezing may be needed, especially for muscle.) OCT is not a cryoprotectant; it's goop that surrounds the specimen and enters cracks and spaces but it does not penetrate. OCT serves to hold each section together during transfer from the cryostat's knife onto a glass slide or coverslip. For some more information (much of it derived from Histonet) have a look at the Biological Stain Commission's FAQ, at https://biologicalstaincommission.org/faqlist.htm#CRYPRO. John Kiernan https://www.schulich.uwo.ca/anatomy//people/faculty/emeriti/kiernan_john.html = = = ________________________________ From: Tyrone Genade via Histonet Sent: November 30, 2023 9:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] O.C.T. what MW PVA and PEG? Hello, I'm using a cryoprotection protocol that involves 3-stage cryoprotection of 15% glucose O/N, then 30% + 50% OCT O/N and then finally O/N in OCT. Compared to previous protocols this works very well -- even when cutting through eyes and lenses (which had previously given a lot of grief). My issue is that preparing the 30% + 50% OCT is a schlep. The OCT puts up a lot of resistance against mixing with the 60% sucrose. It would be much simpler if I could prepare 30% sucrose with powdered PVA and PEG. Does anyone know what MW polymers of PVA and PEG to use and what concentrations to approximate commercial (Scigen Tissue-Plus) OCT? Thanks Tyrone Genade Ph.D. Quillen College of Medicine ETSU Johnson City, TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Tyrone Genade via Histonet Sent: November 30, 2023 9:46 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] O.C.T. what MW PVA and PEG? Hello, I'm using a cryoprotection protocol that involves 3-stage cryoprotection of 15% glucose O/N, then 30% + 50% OCT O/N and then finally O/N in OCT. Compared to previous protocols this works very well -- even when cutting through eyes and lenses (which had previously given a lot of grief). My issue is that preparing the 30% + 50% OCT is a schlep. The OCT puts up a lot of resistance against mixing with the 60% sucrose. It would be much simpler if I could prepare 30% sucrose with powdered PVA and PEG. Does anyone know what MW polymers of PVA and PEG to use and what concentrations to approximate commercial (Scigen Tissue-Plus) OCT? Thanks Tyrone Genade Ph.D. Quillen College of Medicine ETSU Johnson City, TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet