[Histonet] baking after coverslipping

[Michelle Bell]

We baked our slides in our IHC oven on metal trays (for cytology) overnight so we could file them.  15 minutes wouldn’t have worked, but maybe they are using a hotter oven.  As for the air pockets, the baking won’t solve that.  This usually (but not always) happens when the ratio of mounting medium to xylene/xylene sub is not high enough (you need more glue or less xylene).  I hope this helps!
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From: Nancy Schmitt via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, May 19, 2023 9:09:10 AM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] baking after coverslipping

Hello and Happy Friday!
Can you tell me about baking slides to dry the medium? I have not heard of this - we use a Leica CV5030 and then store the slides in order for several days to ensure they are dry before filing.  We are experiencing some "dry back"  (air under coverslip) issues and had someone recommend baking after coverslipping for 15 minutes and then they are ready to file immediately after being read.  We are working with Leica technical on this issue, but this also came up in the process.   Package inserts do not lean to this.  I have been in the same lab for a long time and will appreciate your feedback!
Nancy Schmitt, MLT(ASCP) CM HT CM
Pathology Support Services Supervisor

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