[Histonet] Question about 72 hour upper fixation time for HER2

[Whitaker, Bonnie]

Well, I would worry much less about over-fixation than I would under-fixed tissues.  Do you not have the option to have a weekend processing schedule that runs RT formalin?

Is your volume sufficient you could do an analysis of weekend vs non-weekend ER, PR, HER2 rates and see if they are statistically different?  You should be keeping the overall statistics already.  If they are not statistically different, that should ease your mind,

Good luck!
Bonnie Whitaker




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________________________________
From: Tony Auge via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, May 5, 2023 6:50:00 PM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Question about 72 hour upper fixation time for HER2

Hi Histonet,

Does anyone know if the 72 hour upper fixation time for HER2 was calculated
at room temperature? My lab is currently running our formalin at 40°c and
we go 72 hours on the long weekends, so I'm worried it might be overfixed.

Thanks,
Tony

--

Tony Auge HTL (ASCP)CM QIHC
Cell: (651) 373-4768
Email: tony.auge at gmail.com
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