From relia1 at earthlink.net Tue May 2 10:59:19 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 2 May 2023 11:59:19 -0400 Subject: [Histonet] RELIA HOT Job Alert PhD Level Histologist needed for Research company in Philadelphia area. Can you help? Message-ID: <1cd501d97d0f$0f1c61c0$2d552540$@earthlink.net> Hi Histonetters! I hope you are having a fantastic day! I have been engaged on a search for a PhD level histologist for a research lab in the Philadelphia area. My client is seeking someone who holds a PhD and has strong histology and imaging experience. This is a full-time permanent position and my client offers an excellent compensation package, a beautiful lab and a fantastic team that is eager to welcome you. If you would like more information, please contact me. The quickest way to reach me is via email at relia1 at earthlink.net or on my cell at 407-353-5070. I can also be reached toll free at 866-607-3542. Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Wed May 3 11:05:44 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 3 May 2023 12:05:44 -0400 Subject: [Histonet] Is Your Next Histology Hire on THIS List? RELIA HOT Histology Candidate Report! Message-ID: <019701d97dd9$1eeb90b0$5cc2b210$@earthlink.net> Hello Histopeeps, I hope you are having a fantastic week. All rested and recharged from your lab week celebrations? Welcome to my new program RELIA?s HOT HISTOLOGY CANDIDATE PROGRAM Once a month, I will be bringing you a list of my hottest candidates. That way if someone looks interesting you can let me know. I can?t tell you about everyone I am working with but I will give you a monthly list of the best of the best. All you have to do is contact me email/ call regarding: 1. A candidate on my list OR 2. What I should be on the lookout for, for YOU! MAY 2023 HOT HISTOLOGY CANDIDATES: Washington: ?ASCP elig. Mohs Tech NY NJ PA: NY lic ASCP elig Histotech California: ASCP HT/QIHC Supervisor California: ?ASCP HT/QIHC Histotech California: ?ASCP HT/QIHC IHC specialist Florida: ASCP HT/QIHC Supervisor Tennessee: ASCP HTL elig Histotech Wisconsin: ASCP HTL elig Histotech Minnesota: ASCP HTL elig Histotech Carolinas: ASCP HTL elig Histotech Carolinas: ASCP HT Histotech Florida: ?FL licensed QIHC Histotech Wyoming: ASCP certified Histotech Wisconsin: ASCP HT, QIHC Histotech Texas: ASCP HT, QIHC Histotech ? All of these candidates have histology experience and if they are ASCP elig most have exam dates scheduled. These candidates are specifically looking for Permanent Positions. For more information just shoot me an email to relia1 at earthlink.net or give me a call on my cell at 407-353-5070 I can also send you information on how my services work. If you think of anyone else who might have a need, Would you please pass this along? Thanks-Pam Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Thu May 4 10:34:21 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 4 May 2023 11:34:21 -0400 Subject: [Histonet] Tune in and Check it OUT! I will be appearing on Tech Talks Sunday May 7th at 3pm eastern! Message-ID: <014801d97e9d$e69526c0$b3bf7440$@earthlink.net> Hi Histopeeps! I am so excited to share that I have been invited to be a guest on Tech Talk. This a you tube channel created by Shaneka Howard and it's called The Lab Girl Circle. On the episode with me we will be talking all things histology careers and job hunting. It will air live at 3pm eastern on Sunday 5/7. And you can also catch the replay. Here's the link: https://www.youtube.com/@theLabgirl Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From Jessica.Piche at wtbyhosp.org Fri May 5 08:08:16 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Fri, 5 May 2023 13:08:16 +0000 Subject: [Histonet] SMAD4 antibody Message-ID: Good Morning and Happy Friday! Is anyone running Smad4 on the Dako Omnis? If so, would you be willing to share your protocol? We are having a hard time working this one up. Thank you and have a great day! Jessica Piche, HT(ASCP) Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From SThompson4 at sonichealthcareusa.com Fri May 5 09:01:40 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Fri, 5 May 2023 14:01:40 +0000 Subject: [Histonet] Histology Opportunities Message-ID: <6ebcaab7a97342378e480d283f7a136b@sonichealthcareusa.com> Have you ever thought of living in the great state of New Hampshire? Sonic Healthcare USA, our Seacoast Lab in Exeter, has a great opportunity. This is a family friendly atmosphere, love to help people learn, a beautiful lab and the outdoors are even better! The best beaches are not far away and the great White Mountains are located here. Please reach out to me if you would like to have a conversation with the manager. Never hurts to see what is out there and what we have to offer. www.sonichealthcareusa.com Stephanie Thompson - 210-428-1646 Sthompson4 at sonichealthcareusa.com This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From SThompson4 at sonichealthcareusa.com Fri May 5 12:09:40 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Fri, 5 May 2023 17:09:40 +0000 Subject: [Histonet] Histotech opportunity Message-ID: <465fd07b11f244bcaf006fabef5605f9@sonichealthcareusa.com> Forgot to mention - No sales tax or state tax - can things get any better?! Have you ever thought of living in the great state of New Hampshire? Sonic Healthcare USA, our Seacoast Lab in Exeter, has a great opportunity. This is a family friendly atmosphere, love to help people learn, a beautiful lab and the outdoors are even better! The best beaches are not far away and the great White Mountains are located here. Please reach out to me if you would like to have a conversation with the manager. Never hurts to see what is out there and what we have to offer. www.sonichealthcareusa.com> Stephanie Thompson - 210-428-1646 Sthompson4 at sonichealthcareusa.com This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From kstoll at mcw.edu Fri May 5 12:09:31 2023 From: kstoll at mcw.edu (Stoll, Kathryn) Date: Fri, 5 May 2023 17:09:31 +0000 Subject: [Histonet] SMAD4 antibody In-Reply-To: References: Message-ID: Hi Jessica, I have worked up a SMAD4 clone B-8 on the Autostainer Link 48 feel free to reach out if you would like the protocol. Kind Regards, Kathy Kathryn Stoll, HT(ASCP) Manager, Precision Medicine Laboratory (PML) Phone: (414) 955-4573 | E-mail: kstoll at mcw.edu Precision Medicine Lab customer service pmlcs at mcw.edu ph:414-955-2550 fax: 414-955-0088 Medical College of Wisconsin | 8701 W Watertown Plank Road, Milwaukee, WI 53226 -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, May 5, 2023 12:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 234, Issue 5 ATTENTION: This email originated from a sender outside of MCW. Use caution when clicking on links or opening attachments. ________________________________ Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!H8mHWRdzp34!__5Kw2oSVLcN5hJKUmROUxDh3kvPmg6-_E6JkTVITSVHTk7Xmw7I6RUjolmF9FvOk1j4181WaIi8qj_3RP6J6R1MK-9L03We62Ppt-Qy$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. SMAD4 antibody (Piche, Jessica) 2. Histology Opportunities (Stephanie L. Thompson) ---------------------------------------------------------------------- Message: 1 Date: Fri, 5 May 2023 13:08:16 +0000 From: "Piche, Jessica" To: Histonet Subject: [Histonet] SMAD4 antibody Message-ID: Content-Type: text/plain; charset="iso-8859-1" Good Morning and Happy Friday! Is anyone running Smad4 on the Dako Omnis? If so, would you be willing to share your protocol? We are having a hard time working this one up. Thank you and have a great day! Jessica Piche, HT(ASCP) Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ------------------------------ Message: 2 Date: Fri, 5 May 2023 14:01:40 +0000 From: "Stephanie L. Thompson" To: "Histonet at lists.utsouthwestern.edu" Subject: [Histonet] Histology Opportunities Message-ID: <6ebcaab7a97342378e480d283f7a136b at sonichealthcareusa.com> Content-Type: text/plain; charset="us-ascii" Have you ever thought of living in the great state of New Hampshire? Sonic Healthcare USA, our Seacoast Lab in Exeter, has a great opportunity. This is a family friendly atmosphere, love to help people learn, a beautiful lab and the outdoors are even better! The best beaches are not far away and the great White Mountains are located here. Please reach out to me if you would like to have a conversation with the manager. Never hurts to see what is out there and what we have to offer. https://urldefense.com/v3/__http://www.sonichealthcareusa.com__;!!H8mHWRdzp34!__5Kw2oSVLcN5hJKUmROUxDh3kvPmg6-_E6JkTVITSVHTk7Xmw7I6RUjolmF9FvOk1j4181WaIi8qj_3RP6J6R1MK-9L03We61nVY_jp$ Stephanie Thompson - 210-428-1646 Sthompson4 at sonichealthcareusa.com This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!H8mHWRdzp34!__5Kw2oSVLcN5hJKUmROUxDh3kvPmg6-_E6JkTVITSVHTk7Xmw7I6RUjolmF9FvOk1j4181WaIi8qj_3RP6J6R1MK-9L03We62Ppt-Qy$ ------------------------------ End of Histonet Digest, Vol 234, Issue 5 **************************************** From Tom.Walls at propath.com Fri May 5 12:25:35 2023 From: Tom.Walls at propath.com (Tom Walls) Date: Fri, 5 May 2023 17:25:35 +0000 Subject: [Histonet] Histotech opportunity In-Reply-To: <465fd07b11f244bcaf006fabef5605f9@sonichealthcareusa.com> References: <465fd07b11f244bcaf006fabef5605f9@sonichealthcareusa.com> Message-ID: I'm down here in Dallas if anyone's looking for opportunities this way and we're also Sonic Healthcare Pathology Practice? How's it going in the Great NE Stephanie? Thanks! Tom Walls Histology Manager | ProPath ProPath (A Sonic Healthcare Anatomic Pathology Practice) P 214.237.1792 | M 253.439.8402 | A 1355 River Bend Drive, Dallas, TX 75247 http://www.propath.com/ -----Original Message----- From: Stephanie L. Thompson via Histonet Sent: Friday, May 5, 2023 12:10 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Histotech opportunity [CAUTION - EXTERNAL EMAIL: Do not click any links or open any attachments unless you trust the sender and know the content is safe.] Forgot to mention - No sales tax or state tax - can things get any better?! Have you ever thought of living in the great state of New Hampshire? Sonic Healthcare USA, our Seacoast Lab in Exeter, has a great opportunity. This is a family friendly atmosphere, love to help people learn, a beautiful lab and the outdoors are even better! The best beaches are not far away and the great White Mountains are located here. Please reach out to me if you would like to have a conversation with the manager. Never hurts to see what is out there and what we have to offer. http://www.sonichealthcareusa.com/> Stephanie Thompson - 210-428-1646 Sthompson4 at sonichealthcareusa.com This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.auge at gmail.com Fri May 5 17:50:00 2023 From: tony.auge at gmail.com (Tony Auge) Date: Fri, 5 May 2023 15:50:00 -0700 Subject: [Histonet] Question about 72 hour upper fixation time for HER2 Message-ID: Hi Histonet, Does anyone know if the 72 hour upper fixation time for HER2 was calculated at room temperature? My lab is currently running our formalin at 40?c and we go 72 hours on the long weekends, so I'm worried it might be overfixed. Thanks, Tony -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.auge at gmail.com From Bonnie.Whitaker at osumc.edu Fri May 5 18:03:28 2023 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Fri, 5 May 2023 23:03:28 +0000 Subject: [Histonet] Question about 72 hour upper fixation time for HER2 In-Reply-To: References: Message-ID: Well, I would worry much less about over-fixation than I would under-fixed tissues. Do you not have the option to have a weekend processing schedule that runs RT formalin? Is your volume sufficient you could do an analysis of weekend vs non-weekend ER, PR, HER2 rates and see if they are statistically different? You should be keeping the overall statistics already. If they are not statistically different, that should ease your mind, Good luck! Bonnie Whitaker Get Outlook for iOS ________________________________ From: Tony Auge via Histonet Sent: Friday, May 5, 2023 6:50:00 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Question about 72 hour upper fixation time for HER2 Hi Histonet, Does anyone know if the 72 hour upper fixation time for HER2 was calculated at room temperature? My lab is currently running our formalin at 40?c and we go 72 hours on the long weekends, so I'm worried it might be overfixed. Thanks, Tony -- Tony Auge HTL (ASCP)CM QIHC Cell: (651) 373-4768 Email: tony.auge at gmail.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!Epz7EVxum_BBLGlC1F9yPYzvPqZ6cDG6dn0wpGokoRvtLMb4pzfZEcaz-WfshQ7bT2pZMEgcR4PlPiRpDwpYlmTtVTIKV-43NWO13A$ From kdean70 at hotmail.com Sat May 6 10:27:01 2023 From: kdean70 at hotmail.com (Ken M) Date: Sat, 6 May 2023 15:27:01 +0000 Subject: [Histonet] Sections sticking to tweezers Message-ID: Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken From patpxs at gmail.com Sat May 6 21:57:22 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Sun, 7 May 2023 02:57:22 +0000 (UTC) Subject: [Histonet] Sections sticking to tweezers In-Reply-To: References: Message-ID: <1012189872.694609.1683428242683@mail.yahoo.com> Hi Ken, A few questions: 1. What is the melting point of the paraffin you use???2. Is there a specific reason the temperature of your water bath is 38.5 degrees???3. Do your forceps have any type of imperfections? Bumpy, or ridged? Something that could snag on the sections? If there isn't a specific reason for the water bath temperature to be that low (cutting brain sections), I'm thinking that is playing a large part in your sticky sections. It is common to have the water bath 5 - 10 degrees below the melting point of the wax. It helps the sections stretch out from the compression that happens when being cut. Let's see what others have to say about your sticky situation. Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote:Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote: Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From VKurth at uwhealth.org Mon May 8 07:04:06 2023 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Mon, 8 May 2023 12:04:06 +0000 Subject: [Histonet] Sections sticking to tweezers In-Reply-To: References: Message-ID: You may try to turn your water bath down a few degrees and see if this helps and what is the melting point of the paraffin you are using? Most likely a paraffin/heat situation. If I run into this issue, a quick fix is I rinse my forceps into the ice water, wipe, and the sections will not stick. Good Luck. Ginny Kurth UWHEALTH -----Original Message----- From: Ken M via Histonet Sent: Saturday, May 6, 2023 10:27 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sections sticking to tweezers WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Taylor.Rosa at crl.com Mon May 8 08:35:33 2023 From: Taylor.Rosa at crl.com (Rosa, Taylor) Date: Mon, 8 May 2023 13:35:33 +0000 Subject: [Histonet] Sections sticking to tweezers In-Reply-To: <1012189872.694609.1683428242683@mail.yahoo.com> References: <1012189872.694609.1683428242683@mail.yahoo.com> Message-ID: Hi Ken, Try chilling the tips of the forceps (I usually keep mine on the ice bath) - as long as they are cold, they shouldn't stick to the paraffin. Thanks, Taylor C. Rosa, MS Research Scientist I Pathology Services | Charles River 4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703 P: 919.206.7041 | F: 919.206.7001 taylor.rosa at crl.com | www.criver.com -----Original Message----- From: Paula Sicurello Sent: 06-May-2023 10:57 PM To: Ken M ; Ken M via Histonet Subject: Re: [Histonet] Sections sticking to tweezers Hi Ken, A few questions: 1. What is the melting point of the paraffin you use???2. Is there a specific reason the temperature of your water bath is 38.5 degrees???3. Do your forceps have any type of imperfections? Bumpy, or ridged? Something that could snag on the sections? If there isn't a specific reason for the water bath temperature to be that low (cutting brain sections), I'm thinking that is playing a large part in your sticky sections. It is common to have the water bath 5 - 10 degrees below the melting point of the wax. It helps the sections stretch out from the compression that happens when being cut. Let's see what others have to say about your sticky situation. Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote:Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken_______________________________________________Histonet mailing listHistonet at lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet Paula Sicurello On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet wrote: Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas? Ken _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From umbellas at hotmail.com Tue May 9 05:45:30 2023 From: umbellas at hotmail.com (Gerard Spoelstra) Date: Tue, 9 May 2023 18:45:30 +0800 Subject: [Histonet] Sections sticking to tweezers Message-ID: Hi Ken There is no magic answer to this. Just persistence and experience. Dexterity will improve over time. Gerard From relia1 at earthlink.net Tue May 9 12:12:35 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 9 May 2023 13:12:35 -0400 Subject: [Histonet] The Lab Girl Circle and a Customized Career Search Strategy! Message-ID: <01ac01d98299$73963010$5ac29030$@earthlink.net> Hello Histopeeps!! I hope you are having a great day! Have you heard of The Lab Girl Circle? The Lab Girl Circle is a YouTube Channel for all things histology!! The creator of The Lab Girl Circle is Shaneka Howard. Shan interviewed me on Sunday afternoon and we discussed histology careers and I even gave her a few gems from my upcoming article on travel histology. If you want to check out my interview here is the link: https://www.youtube.com/live/biqNOn5OC-M?feature=share I really encourage you to check out her channel as whole. If you would like to be a guest on Tech Talks shoot me back an email and I will connect you with Shan! Histopeeps!! If you or someone you know is looking for a new opportunity... Check it out! * How about a CUSTOMIZED Search for your NEXT Career Move? * You and I will map out what you need for your next career move. * Whether that is Now or Later!! Here?s why my clients are AWESOME: All of these are full time permanent positions My clients are offering stellar compensation, benefits and bonuses up to 15K! Some Are Paying As Much Or More Than Travel Positions! Most are offering some sort of relocation/sign on bonus They are willing to accommodate your travel plans and/or completion of your travel assignment. My clients want to show you the opportunities they have to offer! Do MORE than just CUT and EMBED!! Here are the types of positions I am recruiting for: *Supervisor* *Lead tech* *Dermpath HT/HTL* *GI HT/HTL *Histotechnician/Histotechnologist" HT/HTL or elig. Histotechs needed in: Clinical, Biotech, AP, Oncology and Research Settings! I have BRAND NEW Labs and Established Labs La Here are the locations: Florida California Washington Tennessee Arizona New York Iowa Most of these are RELIA Exclusives! That?s right you will only see them HERE! And New Opportunities are coming in on a daily basis!! Again, ALL of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses up to 15K!! and of course a great team to work with!! I really appreciate you taking the time to read this post and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. * I can be reached toll free at 866-607-3542 866-60RELIA * Text or call me on my cell at 407-353-5070. * Or email me at: relia1 at earthlink.net ? I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #hmuhistopeeps ? From pruegghm at hotmail.com Wed May 10 15:31:36 2023 From: pruegghm at hotmail.com (Patsy Ruegg) Date: Wed, 10 May 2023 20:31:36 +0000 Subject: [Histonet] Histonet Digest, Vol 234, Issue 10 In-Reply-To: References: Message-ID: Aren't they going to offend the mail histotechs with that name? Just kidding. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 pruegghm at hotmail.com Doug Ruegg C720-281-5407 douglasr19 at hotmail.com ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, May 10, 2023 11:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 234, Issue 10 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From SThompson4 at sonichealthcareusa.com Wed May 10 17:08:45 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Wed, 10 May 2023 22:08:45 +0000 Subject: [Histonet] South Carolina Opportunity Message-ID: <731e2eb7ee204e1e8ef4cd9ab50b6ca1@sonichealthcareusa.com> Sonic Healthcare USA is opening up a position for a histology technician in South Carolina at our Biopsy Diagnostics lab. We would like to be able to present some candidates before the position is posted. Let me know if you are interested. Nice lab and great people! Relocation fee for the right candidate. Send your resume to sthompson4 at sonichealthcareusa.com Thank you - Stephanie Thompson This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From relia1 at earthlink.net Thu May 11 12:46:50 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 11 May 2023 13:46:50 -0400 Subject: [Histonet] ICYMI: The Lab Girl Circle and a customized career strategy! Message-ID: <014901d98430$916da940$b448fbc0$@earthlink.net> Hello Histopeeps!! I hope you are having a great day! Have you heard of The Lab Girl Circle? The Lab Girl Circle is a YouTube Channel for all things histology!! The creator of The Lab Girl Circle is Shaneka Howard. Shan interviewed me on Sunday afternoon, and we discussed histology careers, and I even gave her a few gems from my upcoming article on travel histology. If you want to check out my interview here is the link: https://www.youtube.com/live/biqNOn5OC-M?feature=share I really encourage you to check out her channel as whole. If you would like to be a guest on Tech Talks shoot me back an email and I will connect you with Shan! Histopeeps!! If you or someone you know is looking for a new opportunity... Check it out! * How about a CUSTOMIZED Search for your NEXT Career Move? * You and I will map out what you need for your next career move. * Whether that is Now or Later!! Here?s why my clients are AWESOME: * All of these are full time permanent positions. * My clients are offering stellar compensation, benefits, and bonuses up to 15K! * Some Are Paying As Much Or More Than Travel Positions! * Most are offering some sort of relocation/sign on bonus. * They are willing to accommodate your travel plans and/or completion of your travel assignment. * My clients want to show you the opportunities they have to offer! * Do MORE than just CUT and EMBED!! Here are the types of positions I am recruiting for: *Supervisor* *Lead tech* *Dermpath HT/HTL* *GI HT/HTL *Histotechnician/Histotechnologist" HT/HTL or elig. ***Histotechs needed in: Clinical, Biotech, AP, Oncology and Research Settings!*** I have BRAND NEW Labs and Established Labs Here are the locations: * Florida * California * Washington * Tennessee * Arizona * New York * Iowa Most of these are RELIA Exclusives! That?s right you will only see them HERE! And New Opportunities are coming in daily!! Again, ALL these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses up to 15K!! and of course, a great team to work with!! I really appreciate you taking the time to read this post and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So, if you think you or someone you know might be interested, please contact me. * I can be reached toll free at 866-607-3542 or 866-60RELIA * Text or call me on my cell at 407-353-5070. * Or email me at: relia1 at earthlink.net ?I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From Charles.Bacon at baystatehealth.org Tue May 16 15:12:40 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Tue, 16 May 2023 20:12:40 +0000 Subject: [Histonet] New HRBP Message-ID: This message is to announce that Drake Dargon, HR Business Partner, will be leaving Baystate Health effective May 16, 2023. for another opportunity. Drake joined the HRBP team in January 2022, providing support to the Pharmacy, Radiology and Laboratory teams. Chanel Duchaine, HRBP, will be taking these areas as her primary client groups effective immediately. Chanel can be reached at 795-7298 or Chanel.Duchaine at baystatehealth.org Thank you, Chuck Bacon, HTL(ASCP)CM Histology Supervisor Baystate Health System 361 Whitney Ave., Holyoke, MA 01040 p:413-322-4786|f: 413-322-4790 Charles.Bacon at baystatehealth.org From mills42 at gmail.com Thu May 18 13:37:16 2023 From: mills42 at gmail.com (Caroline Miller) Date: Thu, 18 May 2023 11:37:16 -0700 Subject: [Histonet] Sections sticking to tweezers In-Reply-To: References: <1012189872.694609.1683428242683@mail.yahoo.com> Message-ID: Yes +1 to the chilling of the forceps, especially when you are just starting out. I often kept two pairs - one I was using and the other chilling. Also agree about turning your waterbath down a little. good luck! Caroline On Mon, May 8, 2023 at 6:35?AM Rosa, Taylor via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Ken, > > Try chilling the tips of the forceps (I usually keep mine on the ice bath) > - as long as they are cold, they shouldn't stick to the paraffin. > > Thanks, > > Taylor C. Rosa, MS > Research Scientist I > Pathology Services | Charles River > 4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703 > P: 919.206.7041 | F: 919.206.7001 > taylor.rosa at crl.com | www.criver.com > > -----Original Message----- > From: Paula Sicurello > Sent: 06-May-2023 10:57 PM > To: Ken M ; Ken M via Histonet < > histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] Sections sticking to tweezers > > Hi Ken, > A few questions: > 1. What is the melting point of the paraffin you use? 2. Is there a > specific reason the temperature of your water bath is 38.5 degrees? 3. Do > your forceps have any type of imperfections? Bumpy, or ridged? Something > that could snag on the sections? > If there isn't a specific reason for the water bath temperature to be that > low (cutting brain sections), I'm thinking that is playing a large part in > your sticky sections. > It is common to have the water bath 5 - 10 degrees below the melting point > of the wax. It helps the sections stretch out from the compression that > happens when being cut. > Let's see what others have to say about your sticky situation. > Paula Sicurello > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > histonet at lists.utsouthwestern.edu> wrote:Can anyone tell me why my > sections are sticking to my tweezers and turning into one long string of > snot when I try to separate them? I have a good pair of curved stainless > tweezers and I am trying both the front and the back and tapping sharply > while allowing the tweezers to open at the separation point. I can separate > one or two, then it sticks and pulls up 7 or 8 sections into one long slimy > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > when I go to 6 it still does it. Any ideas? > Ken_______________________________________________Histonet mailing > listHistonet at lists.utsouthwestern.eduhttp:// > lists.utsouthwestern.edu/mailman/listinfo/histonet > > Paula Sicurello > > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > histonet at lists.utsouthwestern.edu> wrote: Can anyone tell me why my > sections are sticking to my tweezers and turning into one long string of > snot when I try to separate them? I have a good pair of curved stainless > tweezers and I am trying both the front and the back and tapping sharply > while allowing the tweezers to open at the separation point. I can separate > one or two, then it sticks and pulls up 7 or 8 sections into one long slimy > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > when I go to 6 it still does it. Any ideas? > > Ken > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller, M.Sc Histology, Imaging, and Image Analysis Specialist 415 2187297 https://www.linkedin.com/in/mills42/ From kdean70 at hotmail.com Thu May 18 14:19:38 2023 From: kdean70 at hotmail.com (Ken M) Date: Thu, 18 May 2023 19:19:38 +0000 Subject: [Histonet] What they don't want you to know. Message-ID: How can you make some of your Histology equipment last forever? Water bath- fill the water every morning with water at about the same temperature that you operate. Every one of these water bath units contain a heating element that is the biggest source of failure. The less you make it work means you can extend the life of your unit ten times. Heat the water in a microwave or on the stove first. Embedding Center- Same thing. Melt the wax ahead of time in a less draw intensive "coffee pot." before you put it in the chamber. Don't ever crank the temperature of the unit up to melt the wax fast so you can get to work quicker. The heating elements will fail in three times their life. Replacing the heating element in your embedding center will cost you $600-1000 dollars. Replacing the coffee pot $100. Block Trimmer- same thing. Don't let it run too long when you are not using them. They use the exact same silicone heating element. The less it is on the longer it lasts. Cryostat- put it to sleep at minus 10 each night. on weekends turn it off, open the top and let it dry out over the weekend. Don't believe the crap about the seals drying out if you turn it off. Do they dry out in your car in the Winter when the A/C is not running? From jaylundgren at gmail.com Thu May 18 14:51:51 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 18 May 2023 14:51:51 -0500 Subject: [Histonet] Sections sticking to tweezers In-Reply-To: References: <1012189872.694609.1683428242683@mail.yahoo.com> Message-ID: Forceps have to be cool and dry. You can cool them in the ice bath, but you have to dry them off. If the tiniest bit wet, they will stick to your ribbon.. Not just forceps, everything has to be cool and dry. Your blade, your microtome stage/ blade holder, your forceps, your slides, if any of them are warm, you'll have trouble getting/ picking up a ribbon. If you have a drop of water on the back of your blade holder, your ribbon can bunch up, You have to dry off the face of your block before cutting it. If you have water on the front of your blade holder, it can fold your ribbon over before you can get it to the water bath. If you drip water into your open box of slides, it's best just to go home for the day. I've used cold spray to cool off a microtome blade/holder in a hot room. Because a cold ribbon can hit a hot blade holder/stage and bunch up. But you have to wipe off the frost that forms, because... that's water. To the Original Poster: I think it's unprofessional to refer to a patient's specimen as a string of snot. I realize you're trying to be funny, but that's potentially a life changing Cancer diagnosis you have on your waterbath. Keep that in mind. We're supposed to be Medical Laboratory Professionals, and want to get paid as such, so let's try to use professional language in professional settings, which Histonet is. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, May 18, 2023 at 1:48?PM Caroline Miller via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Yes +1 to the chilling of the forceps, especially when you are just > starting out. I often kept two pairs - one I was using and the other > chilling. Also agree about turning your waterbath down a little. > > good luck! > Caroline > > On Mon, May 8, 2023 at 6:35?AM Rosa, Taylor via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi Ken, > > > > Try chilling the tips of the forceps (I usually keep mine on the ice > bath) > > - as long as they are cold, they shouldn't stick to the paraffin. > > > > Thanks, > > > > Taylor C. Rosa, MS > > Research Scientist I > > Pathology Services | Charles River > > 4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703 > > P: 919.206.7041 | F: 919.206.7001 > > taylor.rosa at crl.com | www.criver.com > > > > -----Original Message----- > > From: Paula Sicurello > > Sent: 06-May-2023 10:57 PM > > To: Ken M ; Ken M via Histonet < > > histonet at lists.utsouthwestern.edu> > > Subject: Re: [Histonet] Sections sticking to tweezers > > > > Hi Ken, > > A few questions: > > 1. What is the melting point of the paraffin you use? 2. Is there a > > specific reason the temperature of your water bath is 38.5 degrees? 3. > Do > > your forceps have any type of imperfections? Bumpy, or ridged? Something > > that could snag on the sections? > > If there isn't a specific reason for the water bath temperature to be > that > > low (cutting brain sections), I'm thinking that is playing a large part > in > > your sticky sections. > > It is common to have the water bath 5 - 10 degrees below the melting > point > > of the wax. It helps the sections stretch out from the compression that > > happens when being cut. > > Let's see what others have to say about your sticky situation. > > Paula Sicurello > > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > > histonet at lists.utsouthwestern.edu> wrote:Can anyone tell me why my > > sections are sticking to my tweezers and turning into one long string of > > snot when I try to separate them? I have a good pair of curved stainless > > tweezers and I am trying both the front and the back and tapping sharply > > while allowing the tweezers to open at the separation point. I can > separate > > one or two, then it sticks and pulls up 7 or 8 sections into one long > slimy > > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > > when I go to 6 it still does it. Any ideas? > > Ken_______________________________________________Histonet mailing > > listHistonet at lists.utsouthwestern.eduhttp:// > > lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Paula Sicurello > > > > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > > histonet at lists.utsouthwestern.edu> wrote: Can anyone tell me why my > > sections are sticking to my tweezers and turning into one long string of > > snot when I try to separate them? I have a good pair of curved stainless > > tweezers and I am trying both the front and the back and tapping sharply > > while allowing the tweezers to open at the separation point. I can > separate > > one or two, then it sticks and pulls up 7 or 8 sections into one long > slimy > > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > > when I go to 6 it still does it. Any ideas? > > > > Ken > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Caroline Miller, M.Sc > Histology, Imaging, and Image Analysis Specialist > 415 2187297 > https://www.linkedin.com/in/mills42/ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren at gmail.com Thu May 18 15:15:42 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 18 May 2023 15:15:42 -0500 Subject: [Histonet] What they don't want you to know. In-Reply-To: References: Message-ID: I came out of the US military and you NEVER turn anything off. Well, the cold plates on the embedding centers, and you empty and clean your water baths, but other than that, you don't even turn the lights off. If, one time, a tech comes from the OR with a specimen at 3 AM, and knocks the 5 headed scope off its table, because he didn't know where the light switch was, you've just wiped out 500 years of your penny pinching savings from the lights. And don't tell me that you've locked the door, because, believe me, they will get the key from security to unload a Megacolon. Not to mention, the tech might get hurt, which is of inestimable cost. Wait until you plug the cryostat in one busy Monday morning and nothing happens. Good luck with your job search. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, May 18, 2023 at 2:19?PM Ken M via Histonet < histonet at lists.utsouthwestern.edu> wrote: > How can you make some of your Histology equipment last forever? > > Water bath- fill the water every morning with water at about the same > temperature that you operate. Every one of these water bath units contain a > heating element that is the biggest source of failure. The less you make it > work means you can extend the life of your unit ten times. Heat the water > in a microwave or on the stove first. > > Embedding Center- Same thing. Melt the wax ahead of time in a less draw > intensive "coffee pot." before you put it in the chamber. Don't ever crank > the temperature of the unit up to melt the wax fast so you can get to work > quicker. The heating elements will fail in three times their life. > Replacing the heating element in your embedding center will cost you > $600-1000 dollars. Replacing the coffee pot $100. > > Block Trimmer- same thing. Don't let it run too long when you are not > using them. They use the exact same silicone heating element. The less it > is on the longer it lasts. > > Cryostat- put it to sleep at minus 10 each night. on weekends turn it off, > open the top and let it dry out over the weekend. Don't believe the crap > about the seals drying out if you turn it off. Do they dry out in your car > in the Winter when the A/C is not running? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mlm11 at cornell.edu Thu May 18 16:55:25 2023 From: mlm11 at cornell.edu (Mary Lou Norman) Date: Thu, 18 May 2023 21:55:25 +0000 Subject: [Histonet] Sections sticking to tweezers In-Reply-To: References: <1012189872.694609.1683428242683@mail.yahoo.com> Message-ID: I have followed some of this thread and I haven't heard this one point: paraffin sticks to paraffin. No matter how dry ( I guess I've put my forceps in the waterbath) or how cold (got nothin' on that), forceps with a bit of paraffin on them could stick to your sections. I've been retired for a while and can't figure out how to get off the histonet, but I do remember that some paraffin is stickier than others. I did eyes and bones at a veterinary school and always used a higher melting point paraffin. A previous good point: waterbath may be too hot for your paraffin. Hot blades? Went through a lot of ice cubes ? Hope this helps!! Happy Thursday! Mary Lou Norman from Freeville, NY ________________________________ From: Jay Lundgren via Histonet Sent: Thursday, May 18, 2023 3:51 PM To: Caroline Miller Cc: Ken M via Histonet ; Rosa, Taylor Subject: Re: [Histonet] Sections sticking to tweezers Forceps have to be cool and dry. You can cool them in the ice bath, but you have to dry them off. If the tiniest bit wet, they will stick to your ribbon.. Not just forceps, everything has to be cool and dry. Your blade, your microtome stage/ blade holder, your forceps, your slides, if any of them are warm, you'll have trouble getting/ picking up a ribbon. If you have a drop of water on the back of your blade holder, your ribbon can bunch up, You have to dry off the face of your block before cutting it. If you have water on the front of your blade holder, it can fold your ribbon over before you can get it to the water bath. If you drip water into your open box of slides, it's best just to go home for the day. I've used cold spray to cool off a microtome blade/holder in a hot room. Because a cold ribbon can hit a hot blade holder/stage and bunch up. But you have to wipe off the frost that forms, because... that's water. To the Original Poster: I think it's unprofessional to refer to a patient's specimen as a string of snot. I realize you're trying to be funny, but that's potentially a life changing Cancer diagnosis you have on your waterbath. Keep that in mind. We're supposed to be Medical Laboratory Professionals, and want to get paid as such, so let's try to use professional language in professional settings, which Histonet is. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, May 18, 2023 at 1:48?PM Caroline Miller via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Yes +1 to the chilling of the forceps, especially when you are just > starting out. I often kept two pairs - one I was using and the other > chilling. Also agree about turning your waterbath down a little. > > good luck! > Caroline > > On Mon, May 8, 2023 at 6:35?AM Rosa, Taylor via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > > Hi Ken, > > > > Try chilling the tips of the forceps (I usually keep mine on the ice > bath) > > - as long as they are cold, they shouldn't stick to the paraffin. > > > > Thanks, > > > > Taylor C. Rosa, MS > > Research Scientist I > > Pathology Services | Charles River > > 4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703 > > P: 919.206.7041 | F: 919.206.7001 > > taylor.rosa at crl.com | www.criver.com > > > > -----Original Message----- > > From: Paula Sicurello > > Sent: 06-May-2023 10:57 PM > > To: Ken M ; Ken M via Histonet < > > histonet at lists.utsouthwestern.edu> > > Subject: Re: [Histonet] Sections sticking to tweezers > > > > Hi Ken, > > A few questions: > > 1. What is the melting point of the paraffin you use? 2. Is there a > > specific reason the temperature of your water bath is 38.5 degrees? 3. > Do > > your forceps have any type of imperfections? Bumpy, or ridged? Something > > that could snag on the sections? > > If there isn't a specific reason for the water bath temperature to be > that > > low (cutting brain sections), I'm thinking that is playing a large part > in > > your sticky sections. > > It is common to have the water bath 5 - 10 degrees below the melting > point > > of the wax. It helps the sections stretch out from the compression that > > happens when being cut. > > Let's see what others have to say about your sticky situation. > > Paula Sicurello > > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > > histonet at lists.utsouthwestern.edu> wrote:Can anyone tell me why my > > sections are sticking to my tweezers and turning into one long string of > > snot when I try to separate them? I have a good pair of curved stainless > > tweezers and I am trying both the front and the back and tapping sharply > > while allowing the tweezers to open at the separation point. I can > separate > > one or two, then it sticks and pulls up 7 or 8 sections into one long > slimy > > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > > when I go to 6 it still does it. Any ideas? > > Ken_______________________________________________Histonet mailing > > listHistonet at lists.utsouthwestern.eduhttp:// > > lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Paula Sicurello > > > > On Sat, May 6, 2023 at 8:27 AM, Ken M via Histonet< > > histonet at lists.utsouthwestern.edu> wrote: Can anyone tell me why my > > sections are sticking to my tweezers and turning into one long string of > > snot when I try to separate them? I have a good pair of curved stainless > > tweezers and I am trying both the front and the back and tapping sharply > > while allowing the tweezers to open at the separation point. I can > separate > > one or two, then it sticks and pulls up 7 or 8 sections into one long > slimy > > string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even > > when I go to 6 it still does it. Any ideas? > > > > Ken > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Caroline Miller, M.Sc > Histology, Imaging, and Image Analysis Specialist > 415 2187297 > https://www.linkedin.com/in/mills42/ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akuvetpatoloji at gmail.com Fri May 19 05:18:07 2023 From: akuvetpatoloji at gmail.com (Vet Patoloji) Date: Fri, 19 May 2023 13:18:07 +0300 Subject: [Histonet] cd34, cd117 (ckit) and dog-1 Message-ID: Hello histonetters, I'm looking for some antibodies that are working on feline (cat) tissues for ihc. I would be very happy if you could help me with this. Best regards. -- Assoc Prof M. Fatih Bozkurt, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine From Nancy.Schmitt at mercyhealth.com Fri May 19 08:09:10 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 19 May 2023 13:09:10 +0000 Subject: [Histonet] baking after coverslipping Message-ID: Hello and Happy Friday! Can you tell me about baking slides to dry the medium? I have not heard of this - we use a Leica CV5030 and then store the slides in order for several days to ensure they are dry before filing. We are experiencing some "dry back" (air under coverslip) issues and had someone recommend baking after coverslipping for 15 minutes and then they are ready to file immediately after being read. We are working with Leica technical on this issue, but this also came up in the process. Package inserts do not lean to this. I have been in the same lab for a long time and will appreciate your feedback! Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From beth.oneil1 at wvumedicine.org Fri May 19 13:23:31 2023 From: beth.oneil1 at wvumedicine.org (O'Neil, Beth) Date: Fri, 19 May 2023 18:23:31 +0000 Subject: [Histonet] Histo Chill tissue freezing bath Message-ID: We were given a brand new, neve used, FTS SP Scientific Histo Chill tissue freezing bath. This will be great for our department since we won't have to deal with liquid nitrogen. Our problem is we have no idea how to use it. The company doesn't provide installation or training just an operator manual. I am unable to find any videos on the internet so I'm reaching out to anyone who has used this piece of equipment and if anyone knows whether it is not even worth trying to use. We would love to use it for freezing our muscle biopsies for neuropath workups. Thank you Beth O'Neil, MT(ASCP)SC, HTLcm, QIHCcm Histology supervisor/technical specialist beth.oneil1 at wvumedicine.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gu.lang at gmx.at Sat May 20 07:46:23 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 20 May 2023 14:46:23 +0200 Subject: [Histonet] history of H&E staining Message-ID: <000001d98b19$15d4a140$417de3c0$@gmx.at> Hi all! Does anybody know, when the H&E stain became that dominant routine-stain in the pathology labs? It was introduced by Wissowzky 1876, but I am curious when our usual histoprocess became worldwide standard. Regards Gudrun Lang From Kelly.Pairan at ohiohealth.com Mon May 22 10:46:39 2023 From: Kelly.Pairan at ohiohealth.com (Pairan, Kelly) Date: Mon, 22 May 2023 15:46:39 +0000 Subject: [Histonet] Collecting CE data from associates Message-ID: Good morning Histoland, I am trying to see how other institutions collect their associates continuing education information and how you store the data? I am new to my organization and this is my first time in an organization that has so many employees. I've been asked to look our process and I figure you all might have some great suggestions. Thanks, Kelly Kelly Pairan, HT(ASCP)CM, HQIPCM Technical Scientist, Anatomic Pathology and Cytology OhioHealth Laboratory Services From mcokertx at gmail.com Mon May 22 15:58:39 2023 From: mcokertx at gmail.com (Michelle Bell) Date: Mon, 22 May 2023 20:58:39 +0000 Subject: [Histonet] baking after coverslipping In-Reply-To: References: Message-ID: We baked our slides in our IHC oven on metal trays (for cytology) overnight so we could file them. 15 minutes wouldn?t have worked, but maybe they are using a hotter oven. As for the air pockets, the baking won?t solve that. This usually (but not always) happens when the ratio of mounting medium to xylene/xylene sub is not high enough (you need more glue or less xylene). I hope this helps! ________________________________ From: Nancy Schmitt via Histonet Sent: Friday, May 19, 2023 9:09:10 AM To: Histonet Subject: [Histonet] baking after coverslipping Hello and Happy Friday! Can you tell me about baking slides to dry the medium? I have not heard of this - we use a Leica CV5030 and then store the slides in order for several days to ensure they are dry before filing. We are experiencing some "dry back" (air under coverslip) issues and had someone recommend baking after coverslipping for 15 minutes and then they are ready to file immediately after being read. We are working with Leica technical on this issue, but this also came up in the process. Package inserts do not lean to this. I have been in the same lab for a long time and will appreciate your feedback! Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SThompson4 at sonichealthcareusa.com Tue May 23 10:18:14 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 23 May 2023 15:18:14 +0000 Subject: [Histonet] Sonic Healthcare USA - South Carolina Message-ID: <3bfd70ed57794a57b0eb5f481faa731c@sonichealthcareusa.com> Great histology position in South Carolina at Biopsy Diagnostics! It is a small lab, very little turnover, so this is rare opportunity. Just a half hour from Savannah. The General Manager has been there since 2008, very family oriented and a beautiful place to live. This is a night shift Monday - Friday, 10:00 p.m. - 6:00 a.m. Follow the link below to apply: https://shusa.wd5.myworkdayjobs.com/External/job/950---BxDx/Histology-Technician_REQ-021695 Or contact be directly at: sthompson4 at sonichealthcareusa.com 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From relia1 at earthlink.net Tue May 23 13:06:31 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 23 May 2023 14:06:31 -0400 Subject: [Histonet] Today through June 5th ... A window is RAPIDLY Closing!!! Message-ID: <00f801d98da1$4e7b8830$eb729890$@earthlink.net> Hello Histopeeps! I hope you are having a fantastic week. WHY is June 5th is a very important date? It is the date that the next round of contracts start for travelers. WHAT does that mean for YOU? It means that some of the best and the brightest histotechs are signing commitments to Travel and won't be available for permanent positions till the end of Summer! HOW can you capitalize on this Very Short Window? CALL ME! EMAIL ME! TEXT ME!! Need a histotech, supervisor or manager? They could be on this list: ICYMI: Welcome to my new program RELIA's HOT HISTOLOGY CANDIDATE PROGRAM Regularly, I will be bringing you a list of my hottest candidates. That way if someone looks interesting you can let me know. I can't tell you about all of the histopeeps I am working with, but I will give you a monthly list of the best of the best. All you have to do is contact me email/ call regarding: 1. A candidate on my list OR 2. What I should be on the lookout for, for YOU! MAY 2023 HOT HISTOLOGY CANDIDATES: * Washington: ASCP elig. Mohs Tech * NY NJ PA: NY lic ASCP elig Histotech * California: ASCP HT/QIHC Supervisor * California: ASCP HT/QIHC Histotech * California: ASCP HT/QIHC IHC specialist * Florida: ASCP HT/QIHC Supervisor * Tennessee: ASCP HTL elig Histotech * Wisconsin: ASCP HTL elig Histotech * Minnesota: ASCP HTL elig Histotech * Carolinas: ASCP HTL elig Histotech * Carolinas: ASCP HT Histotech * Florida: FL licensed QIHC Histotech * Wyoming: ASCP certified Histotech * Wisconsin: ASCP HT, QIHC Histotech * Texas: ASCP HT, QIHC Histotech I also have experienced Field Support Techs and ASCP certified histotechs open to regions: * East Coast * West Coast * Southern States * Pacific Northwest * Central States All of these candidates have histology experience and if they are ASCP elig most have exam dates scheduled. These candidates are specifically looking for Permanent Positions. If you are in another area and have a need please LET ME KNOW!! I have candidates contacting me daily from all over the US! For more information just shoot me an email to relia1 at earthlink.net or give me a call on my cell at 407-353-5070. I can also send you information on how my services work. If you think of anyone else who might have a need, Would you please pass this along? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From jkiernan at uwo.ca Tue May 23 13:30:13 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 23 May 2023 18:30:13 +0000 Subject: [Histonet] history of H&E staining In-Reply-To: <000001d98b19$15d4a140$417de3c0$@gmx.at> References: <000001d98b19$15d4a140$417de3c0$@gmx.at> Message-ID: Gudrun, your question got me looking through more than a dozen older books, several more recent ones and various articles, but with no clear answer! H&E wasn't a routine combination in 1902. Pathology and normal human histology textbooks in the 1950s show pictures that are clearly H&E but with the stain getting scarcely a mention, and this is also true of the most recent (1999) path text on my shelf. Forty-five alum-haematein mixtures were published between 1868 and 1951. Of these, a majority (26) were in the period 1882-1916 and these include the best-known ones: Delafield, Ehrlich, Mayer, Harris etc, but eosin alone was seldom the recommended counterstain before 1890. H&E has never been the "routine" stain outside the fields of human and veterinary histology and pathology. Other staining combinations are preferred for invertebrates, protozoa, plants and bacteria. My guess is that H&E gradually became "routine" for pathology in the period 1910-1930. If someone has access to some non-technical textbooks from those decades they might be able to narrow down the dates. I could go on and on, with references etc, but this reply may already be too long for Histonet. John Kiernan. = = = ________________________________ From: Gudrun Lang via Histonet Sent: May 20, 2023 8:46 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] history of H&E staining Hi all! Does anybody know, when the H&E stain became that dominant routine-stain in the pathology labs? It was introduced by Wissowzky 1876, but I am curious when our usual histoprocess became worldwide standard. Regards Gudrun Lang _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amyleehisto779 at gmail.com Tue May 23 16:07:04 2023 From: amyleehisto779 at gmail.com (Amy Lee) Date: Tue, 23 May 2023 14:07:04 -0700 Subject: [Histonet] Galectin 9 IHC Message-ID: Hello histonetters, Has anyone done Galectin 9 IHC on human FFPE tissue? Which antibody you recommend? Or do you know any CRO that has this Gal 9 IHC service? Thanks, Wendy From denise.long at uconn.edu Wed May 24 07:00:44 2023 From: denise.long at uconn.edu (Long, Denise M.) Date: Wed, 24 May 2023 12:00:44 +0000 Subject: [Histonet] Room temperature optimal for Histology Lab Message-ID: Good morning, I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. Can anyone point me to a source document? Denise M. Long, MS, HTL (ASCP) QIHC University of Connecticut Department of Pathobiology Connecticut Veterinary Medical Diagnostic Laboratory Histology Laboratory Storrs, Connecticut 06269-3089 (860) 486-0851 From jjohnson at sepalabs.com Wed May 24 08:37:08 2023 From: jjohnson at sepalabs.com (Jennifer Johnson McIntyre) Date: Wed, 24 May 2023 13:37:08 +0000 Subject: [Histonet] Histotech Opportunity in South Georgia - SEPA Labs Message-ID: SEPA Labs, since 1992, is a dynamic CAP accredited laboratory serving multiple regional facilities in Georgia, South Carolina, and Florida. We are committed to providing superior quality anatomical pathology services to our patients and physicians, while exceeding service expectations through our dedicated employees. SEPA Labs is currently seeking an experienced Histotechnician for a full-time night shift opportunity. The histotechnician will prepare histologic slides from tissue sections for microscopic examination and diagnosis by a pathologist. The candidate needs to possess a broad knowledge of the technical aspects of histology including but not limited to section cutting, embedding, frozen section, and special staining. This position is onsite at our main laboratory in Brunswick, Georgia. Brunswick is situated in Glynn County on the southern portion of Georgia's scenic coastline and is the gateway of the Golden Isles. Glynn County is comprised of 4 barrier islands - St. Simons Island, Jekyll Island, Sea Island, and Little St. Simons Island - and the mainland containing the city of Brunswick. Brunswick is separated from the barrier islands by the beautiful Intracoastal Waterway. Whether you're looking for serene marshes, coastal wildlife, breathtaking beaches, world-class golf and fishing, historical landmarks, family activities, quaint shopping, festivals or galleries, the Golden Isles has something for everyone. Learn more about SEPA at www.sepalabs.com For more information or to apply, please visit the careers section of the SEPA Labs website or contact Jennifer McIntyre, jjohnson at sepalabs.com. Jennifer (Johnson) McIntyre, SHRM-CP HR Manager, SEPA Labs Phone: (912) 261-2669, ext. 1059 Fax: (912) 261-0753 Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Chief Privacy Officer at PathGroup immediately at (800)-366-5847. Thank you From mcokertx at gmail.com Wed May 24 10:54:02 2023 From: mcokertx at gmail.com (Michelle Bell) Date: Wed, 24 May 2023 15:54:02 +0000 Subject: [Histonet] Room temperature optimal for Histology Lab In-Reply-To: References: Message-ID: Unfortunately, there are no ?best practices? for room conditions that I can find. The only real standard is to follow the manufacturer?s guidelines for reagents AND equipment. There is a reference to ideal ranges on the FDA website, but it?s specifically refers to clinical labs. I look forward to hearing from others on this topic ________________________________ From: Long, Denise M. via Histonet Sent: Wednesday, May 24, 2023 7:00:44 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Room temperature optimal for Histology Lab Good morning, I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. Can anyone point me to a source document? Denise M. Long, MS, HTL (ASCP) QIHC University of Connecticut Department of Pathobiology Connecticut Veterinary Medical Diagnostic Laboratory Histology Laboratory Storrs, Connecticut 06269-3089 (860) 486-0851 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdhannasch at gmail.com Wed May 24 13:27:00 2023 From: jdhannasch at gmail.com (jdhannasch at gmail.com) Date: Wed, 24 May 2023 11:27:00 -0700 Subject: [Histonet] Room temperature optimal for Histology Lab In-Reply-To: References: Message-ID: Hello Denise, I do not have an answer to your question, but as a consideration when it comes to room temperature, it?s important to keep in mind reagent storage temperature ranges if you are a CAP (and possibly CLIA) accredited lab. If you have a reagent that says it should be stored 20C (68F) to 25C (77F) and you set your lab to 67F because (hypothetically) there is scientific literature saying stating that?s the optimal temperature in a histology lab, then when CAP comes and does an inspection, they could site you a deficiency for improper reagent storage. > On May 24, 2023, at 5:00 AM, Long, Denise M. wrote: > > ? > Good morning, > I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. > Can anyone point me to a source document? > > > Denise M. Long, MS, HTL (ASCP) QIHC > University of Connecticut > Department of Pathobiology > Connecticut Veterinary Medical Diagnostic Laboratory > Histology Laboratory > Storrs, Connecticut 06269-3089 > (860) 486-0851 > From denise.long at uconn.edu Wed May 24 13:28:09 2023 From: denise.long at uconn.edu (Long, Denise M.) Date: Wed, 24 May 2023 18:28:09 +0000 Subject: [Histonet] Room temperature optimal for Histology Lab In-Reply-To: References: Message-ID: Thank you! -----Original Message----- From: jdhannasch at gmail.com Sent: Wednesday, May 24, 2023 2:27 PM To: Long, Denise M. Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Room temperature optimal for Histology Lab *Message sent from a system outside of UConn.* Hello Denise, I do not have an answer to your question, but as a consideration when it comes to room temperature, it?s important to keep in mind reagent storage temperature ranges if you are a CAP (and possibly CLIA) accredited lab. If you have a reagent that says it should be stored 20C (68F) to 25C (77F) and you set your lab to 67F because (hypothetically) there is scientific literature saying stating that?s the optimal temperature in a histology lab, then when CAP comes and does an inspection, they could site you a deficiency for improper reagent storage. > On May 24, 2023, at 5:00 AM, Long, Denise M. wrote: > > ? > Good morning, > I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. > Can anyone point me to a source document? > > > Denise M. Long, MS, HTL (ASCP) QIHC > University of Connecticut > Department of Pathobiology > Connecticut Veterinary Medical Diagnostic Laboratory Histology > Laboratory Storrs, Connecticut 06269-3089 > (860) 486-0851 > From TNMayer at mdanderson.org Thu May 25 12:10:00 2023 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 25 May 2023 17:10:00 +0000 Subject: [Histonet] Room Temperature for a Histology Lab Message-ID: Have HVAC check the temp in the center of the room as well as by the vents. We had a problem that the equipment produced so much heat that it caused the ribbons to stick together. Using regulations for instrument and reagent storage can validate your request. I would also take into account the number of techs working in the lab at its busiest. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Assistant Professor/Associate Program Director HTL Program School of Health Professions MD Anderson Cancer Center tnmayer at mdanderson.org 713-563-3481 wk 832-710-1837 cell Today's Topics: 1. Re: Room temperature optimal for Histology Lab (jdhannasch at gmail.com) 2. Re: Room temperature optimal for Histology Lab (Long, Denise M.) ---------------------------------------------------------------------- Hello Denise, I do not have an answer to your question, but as a consideration when it comes to room temperature, it?s important to keep in mind reagent storage temperature ranges if you are a CAP (and possibly CLIA) accredited lab. If you have a reagent that says it should be stored 20C (68F) to 25C (77F) and you set your lab to 67F because (hypothetically) there is scientific literature saying stating that?s the optimal temperature in a histology lab, then when CAP comes and does an inspection, they could site you a deficiency for improper reagent storage. > On May 24, 2023, at 5:00 AM, Long, Denise M. wrote: > > ? > Good morning, > I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. > Can anyone point me to a source document? > > > Denise M. Long, MS, HTL (ASCP) QIHC > University of Connecticut > Department of Pathobiology > Connecticut Veterinary Medical Diagnostic Laboratory Histology > Laboratory Storrs, Connecticut 06269-3089 > (860) 486-0851 > ------------------------------ -----Original Message----- From: jdhannasch at gmail.com Sent: Wednesday, May 24, 2023 2:27 PM To: Long, Denise M. Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Room temperature optimal for Histology Lab *Message sent from a system outside of UConn.* Hello Denise, I do not have an answer to your question, but as a consideration when it comes to room temperature, it?s important to keep in mind reagent storage temperature ranges if you are a CAP (and possibly CLIA) accredited lab. If you have a reagent that says it should be stored 20C (68F) to 25C (77F) and you set your lab to 67F because (hypothetically) there is scientific literature saying stating that?s the optimal temperature in a histology lab, then when CAP comes and does an inspection, they could site you a deficiency for improper reagent storage. > On May 24, 2023, at 5:00 AM, Long, Denise M. wrote: > > ? > Good morning, > I'm looking for any scientific based information about optimal room temperature and humidity ranges for a Histology lab. I understand it all depends on your paraffin, but I need something for my meeting with HVAC. > Can anyone point me to a source document? > > > Denise M. Long, MS, HTL (ASCP) QIHC > University of Connecticut > Department of Pathobiology > Connecticut Veterinary Medical Diagnostic Laboratory Histology > Laboratory Storrs, Connecticut 06269-3089 > (860) 486-0851 > ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!gMrmmwKoMN0yGfI8RErXQoDay8cR9PQfbv_TRxLNxZA5hQwIdoLMJwyMpW8m3ToWDB5Uu8ZKIPyYP948Ffa8VnZOLbU5J9NmAhHWiHQvDGk$ ------------------------------ End of Histonet Digest, Vol 234, Issue 19 ***************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From SThompson4 at sonichealthcareusa.com Tue May 30 09:31:57 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 30 May 2023 14:31:57 +0000 Subject: [Histonet] Histology Technician Message-ID: <04fa8309a12f4bcfa95cb7903ff7997a@sonichealthcareusa.com> Quality is in our DNA -- is it in yours? You are a superhero when it comes to patient specimens. You've got problem-solving instincts, a passion for patient care, and the drive to keep things running smoothly. You're also looking for great benefits, the support of an all-star team, and an opportunity to grow your career. Join our front line of #HealthcareHeroes! Our mission is to advance the health and wellbeing of our communities as a leader in clinical laboratory solutions. Location: Ridgeland, SC Days: Monday - Friday Hours: 10:00 P.M. - 6:00 A.M. Full-time: Benefit Eligible In this role, you will: As a Histology Tech, you will be in lab professional role, preparing histologic slides from human tissue sections for microscopic examination and diagnosis by Pathologist. This is a great opportunity, the turn-over is very low at this lab, so it is rare they have a vacancy. This is because it is a great place to work! Don't miss out, contact me at sthompson4 at sonichealthcareusa.com https://shusa.wd5.myworkdayjobs.com/External/job/950---BxDx/Histology-Technician_REQ-021695 Stephanie Thompson - 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From relia1 at earthlink.net Tue May 30 11:57:50 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 30 May 2023 12:57:50 -0400 Subject: [Histonet] ICYMI Asking for more money to stay... Message-ID: <018901d99317$def55050$9cdff0f0$@earthlink.net> Hi Histopeeps! Word on the street is that you can make more money if you travel than if you work in a permanent position. What if I told you that's not an absolute? The most obvious answer would be to change jobs and I have some fantastic opportunities further along in this email. Before we go there consider this: Here is some great advice from "Ask a Manager" This is NOT about accepting a counteroffer or issuing an ultimatum to your employer with an offer in your back pocket. This is truly for the person who doesn't want to leave and feels they are being underpaid! "How to ask my company to pay me more if they want me to stay" how to ask my company to pay me more if they want me to stay - Ask a Manager Histopeeps!! my clients are looking for histology professionals who want to invest in their career and they are willing to invest in YOU! My clients are offering: ? Full time permanent positions ? Relocation and Sign-on bonuses up to 15K ? Top pay and benefits ? Opportunity to learn, grow and use all of your skills Here is a brief summary of the most exciting opportunities I am working on today! If you are looking for a new opportunity and don't see it here shoot me an email and let me know what you are looking for and I will definitely keep an eye out for you!! RELIA's Current Opportunities! Leadership: FL - Fort Myers: CancerDX; Quality Assurance CA - Bakersfield: Pathology; Histology Manager NY Flushing/Queens Lead Tech HT/HTL and Eligible: CA - Aliso Viejo: CancerDX CA - Bakersfield: Clinical Pathology FL - Fort Myers: CancerDX AZ - Tucson: Mohs Tech TN - Nashville: AP/Clinical Pathology TN Kingsport: Clinical Pathology TN Johnson City: Clinical Pathology WA Seattle: Research SC Greenville: Clinical Pathology GA Brunswick: Clinical Pathology OH Dayton: Dermatopathology Most of these are RELIA Exclusives! That's right you will only see them HERE! And New Opportunities are coming in on a daily basis!! Again, ALL of these clients are offering full time permanent positions with excellent compensation packages including VERY competitive pay rates, fantastic benefits, relocation and/or sign on bonuses and a great team to work with!! You Can Still Go On Your Summer Vacation AND Take A New Position!!! I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So, if you think you or someone you know might be interested, please contact me. ? I can be reached toll free at 866-607-3542. 866-60RELIA ? Text or call me on my cell at 407-353-5070. ? Or email me at: relia1 at earthlink.net I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Wed May 31 10:15:07 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 31 May 2023 11:15:07 -0400 Subject: [Histonet] RELIA HOT Job Alert!! First LOOK: New Lab in South Carolina can you help? Message-ID: <001401d993d2$afad84a0$0f088de0$@earthlink.net> RELIA HOT JOB ALERT!! Brand NEW Lab in South Carolina exciting permanent opportunities with a growing lab! Excellent compensation. For more info email me at relia1 at earthlink.net or cell 407-353-5070 I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam