From Jennifer.Wooten at tricore.org Wed Mar 1 16:00:55 2023 From: Jennifer.Wooten at tricore.org (Wooten, Jennifer) Date: Wed, 1 Mar 2023 22:00:55 +0000 Subject: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? In-Reply-To: References: Message-ID: Per the grossing definition in ANP.11610 below, grossing is defined as a tissue examination requiring judgment and knowledge of anatomy. This includes the dissection of the specimen, selection of tissue, and any level of examination/description of the tissue including color, weight, measurement or other characteristics of the tissue. CLIA Regulation 42 CFR Part 493.1489 would apply for high complexity testing personnel qualifications. CAP regulations: ANP.11600 Gross Examination - Qualifications All macroscopic tissue gross examinations are performed by a qualified pathologist or pathology resident, or another qualified physician (see note), or under the supervision of a qualified pathologist. ANP.11605 Gross Examination - Non-Pathologist When individuals other than a pathologist or pathology resident assist in gross examinations, the extent of their activities and the nature of supervision (direct vs. indirect) is defined in a written protocol. ANP.11610 Gross Examination - High Complexity Testing Qualifications If individuals other than a pathologist or pathology resident (or an individual who meets the grossing subspecialty qualifications listed under ANP.11600) assist in gross examinations, such individuals qualify as high complexity testing personnel. NOTE: Grossing is defined as a tissue examination requiring judgment and knowledge of anatomy. This includes the dissection of the specimen, selection of tissue, and any level of examination/description of the tissue including color, weight, measurement or other characteristics of the tissue. The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under the CLIA regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a chemical or biological science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes the following: ? 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes six semester hours of chemistry, six semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination, AND ? Laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, NAACLS, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least three months of recorded laboratory training in each specialty in which the individual performs high complexity testing. Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Quality Assurance Auditor | Quality Systems | Tricore Mobile: 505.440.0158 www.tricore.org -----Original Message----- From: Michelle Bell Sent: Monday, February 27, 2023 4:31 PM To: Donna Emge ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? This is so hard to unpack everything that could be wrong with this situation. The weights the lab assistants take would lead me to the conclusion that this would be part of grossing, because it is reportable. It?s such a slippery slope when you start delegating tasks associated with high complexity testing. If I were the manager, I would reach out to your accrediting agency for guidance and support. ________________________________ From: Donna Emge via Histonet Sent: Monday, February 27, 2023 9:39:08 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? I hope someone here can help. I recently visited a lab that has the lab assistants weigh placentas and document, cut the membrane off in one piece around the placenta, cut the umbilical cord off 1? from the placenta, then weigh again, document the weight again, and add the formalin for the PAs to gross the next day. They consider what the lab assistants are doing as processing, not part of grossing. Is this correct? The PAs and Pathologists would like the lab assistants to do this at our lab. 1. What is the CLIA and CAP actual definition of grossing? Where do I find this? 2. Is what the lab assistants are doing at that lab actually a part of grossing, or are they just processing the placentas by the regulatory definition? Thank you in advance, *Donna Emge, HT (ASCP)* UChicago Medicine | AdventHealth GL Regional Laboratory Manager | Histology Donna.Emge(@)AdventHealth.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!NYT1fPLx!-REi1U3hlLxXlU6VOU2zwjYQ4fWIJK2brz5nJtcx7tWq0cso3vmhg5HNNLAFII6asKDDsm1DiKZHQEtQTNAI3avdXcd8YWljJubdJslT_ff7yig$ ......................................................................................................................... STATEMENT OF CONFIDENTIALITY:This message originates from TriCore, Rhodes Group, or an affiliate organization or representative. Please note that the information contained in this message, including any attachments, is confidential and intended for the use of the named recipient. If the reader of this message is any other than the individual or entity named above or an agent responsible for delivery, you are hereby notified that any inappropriate dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately. Any errant copy of this message must be deleted or destroyed. NOTE: The confidentiality of the information disclosed to you may be protected by law, including Section 24-2B-7 NMSA. State law prohibits you from making further disclosure of such information without specific written consent of the person to whom the information pertains or as otherwise permitted by state law. From jaylundgren at gmail.com Wed Mar 1 17:04:37 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Wed, 1 Mar 2023 17:04:37 -0600 Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. Message-ID: Who sells that block deparaffinizer doohickey that everyone has in their lab. What brand is it? Leica? I don't have one in front of me right now. Thanks, Jay A. Lundgren, M.S., HTL (ASCP) Histotechs are the foot soldiers in the battle against Cancer. From mcokertx at gmail.com Wed Mar 1 21:47:10 2023 From: mcokertx at gmail.com (Michelle Bell) Date: Thu, 2 Mar 2023 03:47:10 +0000 Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. In-Reply-To: References: Message-ID: I don?t know the brand, but they are called paratrimmers. Multiple vendors carry they such as Newcomer or Mercedes. My techs loved them! ________________________________ From: Jay Lundgren via Histonet Sent: Wednesday, March 1, 2023 6:04:37 PM To: histonet Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. Who sells that block deparaffinizer doohickey that everyone has in their lab. What brand is it? Leica? I don't have one in front of me right now. Thanks, Jay A. Lundgren, M.S., HTL (ASCP) Histotechs are the foot soldiers in the battle against Cancer. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera at msu.edu Thu Mar 2 05:29:53 2023 From: portera at msu.edu (Porter, Amy) Date: Thu, 2 Mar 2023 11:29:53 +0000 Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. In-Reply-To: References: Message-ID: <829B3229-A564-4E61-B4EA-F1A9BF2F5E60@msu.edu> Thermofisher Sent from my iPhone > On Mar 1, 2023, at 10:47 PM, Michelle Bell via Histonet wrote: > > ?I don?t know the brand, but they are called paratrimmers. Multiple vendors carry they such as Newcomer or Mercedes. My techs loved them! > ________________________________ > From: Jay Lundgren via Histonet > Sent: Wednesday, March 1, 2023 6:04:37 PM > To: histonet > Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. > > Who sells that block deparaffinizer doohickey that everyone has in their > lab. What brand is it? Leica? I don't have one in front of me right now. > > Thanks, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > Histotechs are the foot soldiers in the battle against Cancer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!2YwyT7w2UAncV4GcUjd53DoC7t5_4G4D6TcCZDz2nsj7pD-aUwTyeJF8Yq5P5XRcCnfTb9LSoBZaqLsbl_RnS9tmyYTgKw$ > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!HXCxUKc!2YwyT7w2UAncV4GcUjd53DoC7t5_4G4D6TcCZDz2nsj7pD-aUwTyeJF8Yq5P5XRcCnfTb9LSoBZaqLsbl_RnS9tmyYTgKw$ From karen.heckford at commonspirit.org Thu Mar 2 07:18:31 2023 From: karen.heckford at commonspirit.org (Karen Heckford CA-San Francisco) Date: Thu, 2 Mar 2023 05:18:31 -0800 Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. In-Reply-To: References: Message-ID: I got mine through Thermo Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you On Wed, Mar 1, 2023 at 3:04?PM Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > USE CAUTION - EXTERNAL EMAIL > > ...................................................................... > Who sells that block deparaffinizer doohickey that everyone has in their > lab. What brand is it? Leica? I don't have one in front of me right now. > > Thanks, > > Jay A. Lundgren, M.S., HTL (ASCP) > > > > Histotechs are the foot soldiers in the battle against Cancer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GHohhm5t61fx5WIqk10A1j1agEowfqUHV4ta3OZ5US9GoWlK5ZYYYzLLhsql0_vxxCDE4T0OtBxGKbplHxK4mah8O7khxSPV-w$ > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From patpxs at gmail.com Thu Mar 2 08:38:17 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 2 Mar 2023 14:38:17 +0000 (UTC) Subject: [Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab. In-Reply-To: References: Message-ID: <1060884019.3363305.1677767897980@mail.yahoo.com> Ted Pella, Inc.? (Support small business)? they sell EM, and Histology items. Paula Sicurello On Thu, Mar 2, 2023 at 5:19 AM, Karen Heckford CA-San Francisco via Histonet wrote: I got mine through Thermo Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you On Wed, Mar 1, 2023 at 3:04?PM Jay Lundgren via Histonet < histonet at lists.utsouthwestern.edu> wrote: > USE CAUTION - EXTERNAL EMAIL > > ...................................................................... > Who sells that block deparaffinizer doohickey that everyone has in their > lab.? What brand is it?? Leica?? I don't have one in front of me right now. > >? Thanks, > >? ? ? Jay A. Lundgren, M.S., HTL (ASCP) > > > > Histotechs are the foot soldiers in the battle against Cancer. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!CqLityr3mSQ!GHohhm5t61fx5WIqk10A1j1agEowfqUHV4ta3OZ5US9GoWlK5ZYYYzLLhsql0_vxxCDE4T0OtBxGKbplHxK4mah8O7khxSPV-w$ > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katie at puyallupderm.com Thu Mar 2 12:53:31 2023 From: katie at puyallupderm.com (Katie Riley) Date: Thu, 2 Mar 2023 10:53:31 -0800 Subject: [Histonet] Alarm system In-Reply-To: References: Message-ID: <1677783213707.6ddbde1706d90bc1@puyallupderm.com> We want to add an alarm/pager system to our tissue processor. Does anyone have a system that they like? If so, can you please tell me the make and who installed it for you? Thanks so much! Katie Riley-Hamilton H.T., M.T.QA Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at PuyallupDerm.com 2622 South Meridian Puyallup, WA 98373 (253) 266-0799 (253) 841-2453 x. 321 Sent from Mail for Windows From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, March 2, 2023 10:01 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 232, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From Charles.Bacon at baystatehealth.org Fri Mar 3 13:05:07 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Fri, 3 Mar 2023 19:05:07 +0000 Subject: [Histonet] Alarm system In-Reply-To: <1677783213707.6ddbde1706d90bc1@puyallupderm.com> References: <1677783213707.6ddbde1706d90bc1@puyallupderm.com> Message-ID: ViewPoint - Mesa Labs Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Katie Riley Sent: Thursday, March 2, 2023 1:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Alarm system We want to add an alarm/pager system to our tissue processor. Does anyone have a system that they like? If so, can you please tell me the make and who installed it for you? Thanks so much! Katie Riley-Hamilton H.T., M.T.QA Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic katie at PuyallupDerm.com 2622 South Meridian Puyallup, WA 98373 (253) 266-0799 (253) 841-2453 x. 321 Sent from Mail for Windows From: histonet-request at lists.utsouthwestern.edu Sent: Thursday, March 2, 2023 10:01 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 232, Issue 1 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!P_vj-BUkwjF5!cnH-_jdwKvTpyI019AyKjh4Q1bYi1vf168LuaR9YoFkwI_AARmqeiGuOnJ2csR62p_IA6jxWX5b7-_hzExoq6ZLqTt_2wOzqNXg7NY7TcS-_96A$ or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From redrose297 at gmail.com Mon Mar 6 19:10:11 2023 From: redrose297 at gmail.com (warda hassan) Date: Tue, 7 Mar 2023 05:10:11 +0400 Subject: [Histonet] IHC systems Message-ID: Dear all We are planning to purchase a new IHC system. May I request your valuable feedback, observation and troubleshooting encountered while handling Leica Bond 3? Would you prefer Leica or Roche for the quality of staining, consumables supply and user-friendly lean management with help in TAT improvement? Kind Regards Thanking you for helping out W Anwar From plucas at biopath.org Tue Mar 7 08:44:59 2023 From: plucas at biopath.org (Paula) Date: Tue, 7 Mar 2023 06:44:59 -0800 Subject: [Histonet] Leica Helicobacter pylori Message-ID: <002a01d95103$65a95c10$30fc1430$@biopath.org> Hello, good morning Has anyone tried this antibody (7 ml predilute) on your system? We have the Bond III and I was hoping to gain some information on where to start the staining parameters for this antibody. Thank you, Paula From rfisher0126 at msn.com Thu Mar 9 08:51:22 2023 From: rfisher0126 at msn.com (Renee Fisher) Date: Thu, 9 Mar 2023 14:51:22 +0000 Subject: [Histonet] Mice/Rat brain Message-ID: We are having issues with mice and rat brain sections. There are folds in the tissue. We just can?t seem to get a flat section without folds. Any suggestions? Sent from my iPhone From patpxs at gmail.com Thu Mar 9 09:04:30 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 9 Mar 2023 15:04:30 +0000 (UTC) Subject: [Histonet] Mice/Rat brain In-Reply-To: References: Message-ID: <1435932590.1558718.1678374270270@mail.yahoo.com> Hi Renee,? Brains are notorious for being difficult.? Processing has to be just so, embedding has to be just so, and sectioning?? Well, just plain tricky. It sounds like your water bath may be a smidge too cool.? Brains are picky that way.? Water bath too warm?? Poof!? The section explodes.? Too cool? Wrinkles.?? Can you use vibratome sections or do you need FFPE sections? Paula Sicurello On Thu, Mar 9, 2023 at 6:51 AM, Renee Fisher via Histonet wrote: We are having issues with mice and rat brain sections. There are folds in the tissue. We just can?t seem to get a flat section without folds. Any suggestions? Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA at mercer.edu Thu Mar 9 09:06:02 2023 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Thu, 9 Mar 2023 15:06:02 +0000 Subject: [Histonet] Georgia Society for Histotechnology 50th Anniversary In-Reply-To: References: Message-ID: Hi Histotechs, Please join us at Sea Palms Resort on St Simons Island, GA for our 50th anniversary meeting. The links to the program and registration are below. Rooms will go fast so make your reservations soon. The hotel rates and phone number to call are on our website at www.georgiahistotech.org These are the Program Schedule link and the Registration Form link. 50th Anniversary Program Schedule 50th Anniversary Registration Form Look forward to seeing ya'll there. Shirley Shirley Powell, HTL(ASCP) Technical Director Histology Curricular Support Laboratory Pathology Department Mercer University School of Medicine powell_sa at mercer.edu From VKurth at uwhealth.org Thu Mar 9 09:32:41 2023 From: VKurth at uwhealth.org (Kurth, Virginia L) Date: Thu, 9 Mar 2023 15:32:41 +0000 Subject: [Histonet] Mice/Rat brain In-Reply-To: References: Message-ID: I remember adding a couple drops of ETOH to the water bath; it's been a while so I am not sure the exact protocol. (it will spin the tissue). Virginia Kurth, HT, (ASCP)CM UW Health Surgical Pathology Madison, WI 53792 -----Original Message----- From: Renee Fisher via Histonet Sent: Thursday, March 9, 2023 8:51 AM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Mice/Rat brain WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. We are having issues with mice and rat brain sections. There are folds in the tissue. We just can't seem to get a flat section without folds. Any suggestions? Sent from my iPhone _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cvkurth%40uwhealth.org%7C0f7664315e804bbb2b9708db20add725%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638139703238228760%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=lxsmJRolw0b9WLAjz%2FGAi9lof5uL2WXOK1zZevHuHNg%3D&reserved=0 From criley at udel.edu Thu Mar 9 13:00:23 2023 From: criley at udel.edu (Charles Riley) Date: Thu, 9 Mar 2023 14:00:23 -0500 Subject: [Histonet] Alcian blue van gieson double stain Message-ID: I am working with a researcher and they saw an article where someone performed an Alcian Blue and elastic van Gieson double stain. If anyone has done this before can you provide your protocol. I am trying to figure out the best way to perform this process to get the best staining results. Here are my questions/thoughts 1. Is it better to run the Alcian blue as usual and then perform the van gieson stain? 2. Will the differentiating solution in the van gieson stain affect the alcian blue staining in any way? From paula at excaliburpathology.com Thu Mar 9 13:18:55 2023 From: paula at excaliburpathology.com (Paula Keene Pierce) Date: Thu, 9 Mar 2023 19:18:55 +0000 (UTC) Subject: [Histonet] Alcian blue van gieson double stain In-Reply-To: References: Message-ID: <351568128.1365458.1678389535149@mail.yahoo.com> Hi, if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com A sharp knife is nothing without a sharp eye. - Klingon Proverb On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet wrote: I am working with a researcher and they saw an article where someone performed an Alcian Blue? and elastic van Gieson double stain.? If anyone has done this before can you provide your protocol.? I am trying to figure out the best way to perform this process to get the best staining results. Here are my questions/thoughts 1. Is it better to run the Alcian blue as usual and then perform the van gieson stain? 2. Will the differentiating solution in the van gieson stain affect the alcian blue staining in any way? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akemiat3377 at gmail.com Thu Mar 9 13:35:03 2023 From: akemiat3377 at gmail.com (Akemi Allison) Date: Thu, 9 Mar 2023 11:35:03 -0800 Subject: [Histonet] Alcian blue van gieson double stain In-Reply-To: <351568128.1365458.1678389535149@mail.yahoo.com> References: <351568128.1365458.1678389535149@mail.yahoo.com> Message-ID: Just curious, why not do a Movat?s Pentachome? It gives you everything you are looking for and more. Best regards, Akemi Allison-Tacha, BS, Ht, HTL Akemiat3377 at gmail.com Sent from my iPhone > On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet wrote: > > ?Hi, > if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson. > Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com > > A sharp knife is nothing without a sharp eye. - Klingon Proverb > > On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet wrote: > > I am working with a researcher and they saw an article where someone > performed an Alcian Blue and elastic van Gieson double stain. If anyone > has done this before can you provide your protocol. I am trying to figure > out the best way to perform this process to get the best staining results. > Here are my questions/thoughts > > 1. Is it better to run the Alcian blue as usual and then perform the van > gieson stain? > 2. Will the differentiating solution in the van gieson stain affect the > alcian blue staining in any way? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tonyreilly55 at gmail.com Thu Mar 9 22:02:45 2023 From: tonyreilly55 at gmail.com (Tony Reilly) Date: Fri, 10 Mar 2023 14:02:45 +1000 Subject: [Histonet] Alcian blue van gieson double stain In-Reply-To: References: Message-ID: Hi Charles I have done this stain many times as an abbreviated version of Movats Pentachrome. You are correct in thinking the van gieson will affect the AB stain due to the acid. As in the Movat stain do AB first then place slides in alcoholic ammonia for 1 hour to convert AB to insoluble Monastral blue then finish with the VVG. Regards Tony Reilly Sent from my iPhone > On 10 Mar 2023, at 5:49 am, Akemi Allison via Histonet wrote: > > ?Just curious, why not do a Movat?s Pentachome? It gives you everything you are looking for and more. > Best regards, > Akemi Allison-Tacha, BS, Ht, HTL > Akemiat3377 at gmail.com > > Sent from my iPhone > >> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet wrote: >> >> ?Hi, >> if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend doing the Verhoff elastic stain first, then the Alcian blue, and then the Van Gieson. >> Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com >> >> A sharp knife is nothing without a sharp eye. - Klingon Proverb >> >> On Thursday, March 9, 2023 at 01:05:14 PM CST, Charles Riley via Histonet wrote: >> >> I am working with a researcher and they saw an article where someone >> performed an Alcian Blue and elastic van Gieson double stain. If anyone >> has done this before can you provide your protocol. I am trying to figure >> out the best way to perform this process to get the best staining results. >> Here are my questions/thoughts >> >> 1. Is it better to run the Alcian blue as usual and then perform the van >> gieson stain? >> 2. Will the differentiating solution in the van gieson stain affect the >> alcian blue staining in any way? >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ltazwell at aol.com Fri Mar 10 06:26:02 2023 From: ltazwell at aol.com (Mr. Lawrence Tazwell Jr.) Date: Fri, 10 Mar 2023 12:26:02 +0000 (UTC) Subject: [Histonet] TAke me off this list References: <1614547190.1111814.1678451162592.ref@mail.yahoo.com> Message-ID: <1614547190.1111814.1678451162592@mail.yahoo.com> From kdean70 at hotmail.com Wed Mar 15 11:51:03 2023 From: kdean70 at hotmail.com (Ken M) Date: Wed, 15 Mar 2023 16:51:03 +0000 Subject: [Histonet] Problems with Melan A staining Message-ID: One of my histotech friends is having issues with Melan A staining. Using MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue control was stained strongly positive while the positive melanoma tissue control was stained weakly positive. He couldn?t figure out why after several tries to reduce background. Can someone give me some suggestions? Thank you very much! Ken From tbraud at holyredeemer.com Wed Mar 15 12:53:25 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Wed, 15 Mar 2023 17:53:25 +0000 Subject: [Histonet] background staining Melan A Message-ID: <22273c18d1a34a2a93b8298ffd1af5af@holyredeemer.com> My first instinct is to ask what detection system you are using. If you are using an Avidin-Biotin detection, kidney is notorious for having excess biotin that must be blocked, or better yet, use a polymer based detection system. That needs to be answered before further trouble shooting. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion Today's Topics: 1. Problems with Melan A staining (Ken M) ---------------------------------------------------------------------- Message: 1 Date: Wed, 15 Mar 2023 16:51:03 +0000 From: Ken M Subject: [Histonet] Problems with Melan A staining One of my histotech friends is having issues with Melan A staining. Using MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue control was stained strongly positive while the positive melanoma tissue control was stained weakly positive. He couldn?t figure out why after several tries to reduce background. Can someone give me some suggestions? Thank you very much! Ken From bianca at alliedsearchpartners.com Wed Mar 15 14:59:11 2023 From: bianca at alliedsearchpartners.com (Bianca Shalagin) Date: Wed, 15 Mar 2023 19:59:11 +0000 Subject: [Histonet] Perm Histotech jobs in Boston, MA Message-ID: Opportunity Alert Histotech Positions available in Boston, MA Allied Search Partners is currently conducting a search for: Histotech - Boston, MA Permanent position Competitive pay All Shift Available Must be ASCP certified or eligible. Bianca Shalagin, Recruiter Allied Search Partners (386) 204-7886 From bianca at alliedsearchpartners.com Wed Mar 15 15:51:50 2023 From: bianca at alliedsearchpartners.com (Bianca Shalagin) Date: Wed, 15 Mar 2023 20:51:50 +0000 Subject: [Histonet] Perm Histotech jobs in Statesboro's, GA and Charleston, SC Message-ID: Opportunity Alert Histotech Positions available in Charleston, SC - Savannah, GA - Billings, MT Allied Search Partners is currently conducting a search for: Histotech - Charleston, SC Permanent position Pay: $30-$34/hr + $2 shift differential for evening shift + $2 if OIHC certified Shift: M-F 2:30pm - 11:00pm Must be ASCP certified or eligible. Histotech - Savannah, GA Area Permanent Position Pay: $30-$34/hr + $2 shift differential for evening shift + $2 if OIHC certified Shift: M-F 7:00am - 3:30pm Must be ASCP certified or eligible. Titus Tyson, Recruiter Allied Search Partners (386) 204-0265 From relia1 at earthlink.net Thu Mar 16 10:34:01 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 16 Mar 2023 11:34:01 -0400 Subject: [Histonet] Travel Histology Fact vs. Fiction and much more! Message-ID: <002501d9581c$bc7b0e10$35712a30$@earthlink.net> Hi Histonetters!! I hope you had a fantastic Histotechnology Professionals Day last Friday. I hope you were celebrated as the hero you are! I want to thank you again for allowing me to be a part of your community since 1995! Since COVID I have received so many inquiries about travel histology and since I don't work in Travel - I work exclusively in Permanent Placement so I couldn't answer those questions and decided to ask for YOU. I am putting the finishing touches on my informal study: Travel Histology Fact vs. Fiction and I will be sending in in this bulletin in the coming weeks so BOLO - Be On The Lookout! If you are a traveler, have been a traveler or know a traveler please weigh in with your experiences. You can do so by replying to this email or shooting one to me directly at relia1 at earthlink.net or give me a call at 407-353-5070. I have heard some amazing stories about the best of travel and some pretty unbelievable stories about the worst. I am really looking forward to sharing my findings with you! I am currently working with some amazing career opportunities. My clients are offering: * Sign on Bonuses and Relocation Assistance * Excellent Benefits and PTO * The opportunity to grow and learn new things * The opportunity to make connections with your team members * Brand New and Growing Labs * The opportunity to MAKE A DIFFERENCE! All of these are full time permanent positions, and my clients offer excellent compensation packages including in most cases a sign on bonus and or relocation assistance. And all of them have fantastic teams ready to welcome you!! LOCATIONS: * Wyoming * Iowa * Pennsylvania * Arizona * South Carolina * Virginia * Florida * California My clients need you for: * Leadership * Quality Assurance * Cancer DX * GI * Derm * Immunohistochemistry * Pathologist's Assistant /Grossing Histotech If you are looking for something new locally or in another area and you don't see it listed here, PLEASE let me know. I got several of these positions in the past few hours!!! I have new positions coming all of the time and would love to be on the lookout for YOU! Remember, refer someone and I place them you earn a referral bonus! Help Your Friends and Help Me and Get Rewarded! If you or someone you know would like more information, please contact me. You can reach me toll free at 866-607-3542 or call/text my cell at 407-353-5070 or email me at relia1 at earthlink.net . I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Have a fantastic Week my friend! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #hmuhistopeeps From c.tague at pathologyarts.com Fri Mar 17 12:00:41 2023 From: c.tague at pathologyarts.com (Curt Tague) Date: Fri, 17 Mar 2023 17:00:41 +0000 Subject: [Histonet] Ventana Special Stain Platform Message-ID: Simple question for you all, is there a market for these machines, I have 2 that we used for only a few months then shelved, I'd like to find a home for them. Before I go through the effort of searching for an aftermarket vendor I am really just curious if anyone uses these things? Thanks, Curt From doolee at shands.ufl.edu Fri Mar 17 16:11:20 2023 From: doolee at shands.ufl.edu (Dooley, Elaine O.) Date: Fri, 17 Mar 2023 21:11:20 +0000 Subject: [Histonet] coverslip machines Message-ID: <3d52bea06cce4d039b31ebe9f2be48e7@shands.ufl.edu> Hi everyone, Does anyone know of a cover slipping machine other than the Roche HE 600, in which the slides are loaded in 20 folder type trays. The slides are not picked up by the machine, rather the coverslip is applied to the slides in the folder. Elaine Dooley Shands Labs at Rocky Point 352-265-0111 ext 72117 Or does anyone have good luck putting slides from a Ventana ultra with clear plastic overlays on an automated coverslipper? Our old coverslippers always had problems with the labels sticking to the robotic arm that lifts the slide. From tbraud at holyredeemer.com Mon Mar 20 08:10:10 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 20 Mar 2023 13:10:10 +0000 Subject: [Histonet] Coverslip Instruments Message-ID: We use the Tissue Tek Glas with our Ventana labeled slides with no problem. The key is to insure the label does not hang off the edge in the slightest. However, it does not put them in a tray, however, that function only takes about 10 seconds. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion Message: 2 Date: Fri, 17 Mar 2023 21:11:20 +0000 From: "Dooley, Elaine O." Subject: [Histonet] coverslip machines Hi everyone, Does anyone know of a cover slipping machine other than the Roche HE 600, in which the slides are loaded in 20 folder type trays. The slides are not picked up by the machine, rather the coverslip is applied to the slides in the folder. Elaine Dooley Shands Labs at Rocky Point 352-265-0111 ext 72117 Or does anyone have good luck putting slides from a Ventana ultra with clear plastic overlays on an automated coverslipper? Our old coverslippers always had problems with the labels sticking to the robotic arm that lifts the slide. From Jessica.Piche at wtbyhosp.org Mon Mar 20 10:24:19 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Mon, 20 Mar 2023 15:24:19 +0000 Subject: [Histonet] Specimens for Gout Message-ID: Good Morning Everyone, I hope you all had a nice weekend. What are people doing to process gout specimens? Are you fixing them in Absolute alcohol (and for how long), and then loading on tissue processor starting in xylene? I'm hearing different things and am curious about what other people are doing. Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From jmacdonald at mtsac.edu Mon Mar 20 14:04:54 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 20 Mar 2023 19:04:54 +0000 Subject: [Histonet] Specimens for Gout In-Reply-To: References: Message-ID: They should be fixed in absolute alcohol and can go on the processor at the absolute alcohol step, or they can go into the xylene if completely fixed and dehydrated. -----Original Message----- From: Piche, Jessica via Histonet Sent: Monday, March 20, 2023 8:24 AM To: Histonet Subject: [Histonet] Specimens for Gout EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Good Morning Everyone, I hope you all had a nice weekend. What are people doing to process gout specimens? Are you fixing them in Absolute alcohol (and for how long), and then loading on tissue processor starting in xylene? I'm hearing different things and am curious about what other people are doing. Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C993300dce3b140492cc308db29574000%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638149226908396581%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=8%2ByWCg9z2rE13ijuOa1kIlMhBLT7xWxxrUbscr9ZHrk%3D&reserved=0 From criley at udel.edu Tue Mar 21 13:30:34 2023 From: criley at udel.edu (Charles Riley) Date: Tue, 21 Mar 2023 14:30:34 -0400 Subject: [Histonet] IHC staining of tendons and cartilage Message-ID: Hello all, I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself during each process. If anyone does staining like this routinely and has some pointers/tricks to try to get the samples to adhere to the slides better it would be greatly appreciated. I have tried using charged slides from a variety of vendors and get similar results across the board. From POWELL_SA at mercer.edu Tue Mar 21 13:56:43 2023 From: POWELL_SA at mercer.edu (Shirley A. Powell) Date: Tue, 21 Mar 2023 18:56:43 +0000 Subject: [Histonet] IHC staining of tendons and cartilage In-Reply-To: References: Message-ID: Hi Charles, Shirley Powell here in humid Georgia. I ran an IHC reference lab here for many years. I had a problem with using charged slides for a lot of the tissues I processed. I used manual and automation methods. My tissues were washing off a lot. I changed to an adhesive for the water bath called Sta-On and I think Surgipath was the company that made it. Surgipath was bought out by Leica but they still sold it. Sta-On was the best adhesive I had found and that worked for me for many years. Whenever I do IHC that is what I use, especially bone, cartilage, bloody specimens, autopsy tissues, they stay on better. Some other companies may be selling it now, like VWR/Avantor. Just Google it. Shirley Shirley Powell, HTL(ASCP) Technical Director Histology Curricular Support Laboratory Pathology Department Mercer University School of Medicine powell_sa at mercer.edu Phone: 478-301-2374 https://medicine.mercer.edu/ -----Original Message----- From: Charles Riley via Histonet Sent: Tuesday, March 21, 2023 2:31 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC staining of tendons and cartilage Hello all, I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself during each process. If anyone does staining like this routinely and has some pointers/tricks to try to get the samples to adhere to the slides better it would be greatly appreciated. I have tried using charged slides from a variety of vendors and get similar results across the board. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C148071115eac460740a508db2a3a6b5e%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C638150202637381264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zZqlEosD%2BmC9X%2FyAsT1k05BSBojNv2uveLaj7nEf730%3D&reserved=0 From jkiernan at uwo.ca Tue Mar 21 16:47:38 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 21 Mar 2023 21:47:38 +0000 Subject: [Histonet] IHC staining of tendons and cartilage In-Reply-To: References: Message-ID: You might like to look at this 1999 article from Microscopy Today, about keeping sections on slides. https://publish.uwo.ca/~jkiernan/adhesivs.htm John Kiernan London, Canada. = = = ________________________________ From: Shirley A. Powell via Histonet Sent: March 21, 2023 2:56 PM To: Charles Riley Cc: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] IHC staining of tendons and cartilage Hi Charles, Shirley Powell here in humid Georgia. I ran an IHC reference lab here for many years. I had a problem with using charged slides for a lot of the tissues I processed. I used manual and automation methods. My tissues were washing off a lot. I changed to an adhesive for the water bath called Sta-On and I think Surgipath was the company that made it. Surgipath was bought out by Leica but they still sold it. Sta-On was the best adhesive I had found and that worked for me for many years. Whenever I do IHC that is what I use, especially bone, cartilage, bloody specimens, autopsy tissues, they stay on better. Some other companies may be selling it now, like VWR/Avantor. Just Google it. Shirley Shirley Powell, HTL(ASCP) Technical Director Histology Curricular Support Laboratory Pathology Department Mercer University School of Medicine powell_sa at mercer.edu Phone: 478-301-2374 https://medicine.mercer.edu/ -----Original Message----- From: Charles Riley via Histonet Sent: Tuesday, March 21, 2023 2:31 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] IHC staining of tendons and cartilage Hello all, I am in a new position and it will potentially require doing a lot of IHC testing on cartilage and tendon samples. I have done some practice runs on my automated stainer and manually and am running into issues with the tissue sections falling off completely or folding over on itself during each process. If anyone does staining like this routinely and has some pointers/tricks to try to get the samples to adhere to the slides better it would be greatly appreciated. I have tried using charged slides from a variety of vendors and get similar results across the board. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpowell_sa%40mercer.edu%7C148071115eac460740a508db2a3a6b5e%7C4fb34d2889b247109bcc30824d17fc30%7C0%7C0%7C638150202637381264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=zZqlEosD%2BmC9X%2FyAsT1k05BSBojNv2uveLaj7nEf730%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Christopher.Hagon at act.gov.au Tue Mar 21 17:16:57 2023 From: Christopher.Hagon at act.gov.au (Hagon, Christopher (Health)) Date: Tue, 21 Mar 2023 22:16:57 +0000 Subject: [Histonet] Tracking of blocks onto processors Message-ID: UNOFFICIAL Hi Histonetters, Just wondering if or how others are tracking/scanning racks of blocks onto processors. In this day and age of traceability with barcodes, we scan each block in each rack before loading onto a processor and recording which processor that rack went on to. Has anyone got a more efficient way of doing this? Assigning at grossing/cut-up is an option, but is an extra step for the PA/registrar and relies on knowing which processor is in use that day. Looking in the crystal ball, will next gen processors automatically scan blocks as the first step? Load blocks, start the run and it produces a report of the blocks on that run? Interested to hear peoples thoughts. Cheers, Chris Hagon - Pathology Digital Solutions Division, ACT Health Directorate ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- From idimenstein at hotmail.com Tue Mar 21 21:41:11 2023 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Wed, 22 Mar 2023 02:41:11 +0000 Subject: [Histonet] Histonet Digest, Vol 232, Issue 11 In-Reply-To: References: Message-ID: >From my Grossing Technology book: Gouty tissue contains monosodium urate crystals. Tissue specimens with gout diagnosis must be submitted in either Carnoy?s fixative (chloroform, acetic acid, and absolute ethanol, now almost abandoned in regular practice) or in absolute ethanol. Fixation cannot be in formalin, as formalin contains water which may dissolve any urate crystals that are present (uric acid is even more soluble in formalin than in water). Once received, the specimen must be processed from 100% ethanol, skipping not only formalin, but also the graded alcohol series in the tissue processor. Some laboratories start with 70% alcohol in microwave-assisted processing. However, urate crystals, particularly in large deposits (tophi), often survive formalin fixation and routine processing anyway. If a gout specimen is received in formalin, it can be recovered by drying the fixative on a slide in an oven and mounting it unstained for Diff-Quick stain. Unstained slide can be viewed under polarized light. By using red filter under polarized light uric acid crystals can also be distinguished from calcium pyrophosphate dihydrate crystals (pseudogout). The rest is in the histotechnology realm. Izak Dimenstein ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, March 21, 2023 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 232, Issue 11 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From Donna.Willis at BSWHealth.org Wed Mar 22 07:29:06 2023 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 22 Mar 2023 12:29:06 +0000 Subject: [Histonet] Tracking of blocks onto processors In-Reply-To: References: Message-ID: Chris, We have been tracking blocks since we went live with Vantage over 10 years ago. Roche (Ventana then) built a custom program for us to use at that time because they had not yet implemented it into Vantage. In the past 2 years Roche has updated their software to allow documenting this information. There are several options of how the scanning is performed. For our lab we scan before we place the block into the formalin holding tanks, right after the PA/Resident/Grossing Tech have placed the tissue into the block. I prefer it done there because I do not want my technicians exposed an extra time to formalin if the scanning is done right before the baskets are placed on the processors. We have a manual log that is used to document the time the cassette are placed in and taken out of the processors. We also track our blocks and slides into storage. I hope this helps. Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, March 21, 2023 5:17 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Tracking of blocks onto processors CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. UNOFFICIAL Hi Histonetters, Just wondering if or how others are tracking/scanning racks of blocks onto processors. In this day and age of traceability with barcodes, we scan each block in each rack before loading onto a processor and recording which processor that rack went on to. Has anyone got a more efficient way of doing this? Assigning at grossing/cut-up is an option, but is an extra step for the PA/registrar and relies on knowing which processor is in use that day. Looking in the crystal ball, will next gen processors automatically scan blocks as the first step? Load blocks, start the run and it produces a report of the blocks on that run? Interested to hear peoples thoughts. Cheers, Chris Hagon - Pathology Digital Solutions Division, ACT Health Directorate ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. You should not copy or use it for any purpose, nor disclose its contents to any other person. ----------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!D9KQ_WYXlaKyGu0_GWULeJZiOiQNKYW9LvDtdi3_5IZkKU3jPn3dc11ipwa621HHvBQFcIpyS0RPtErYJaRQNf_GiX-6SwOw$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From bcooper at chla.usc.edu Wed Mar 22 10:30:04 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 22 Mar 2023 15:30:04 +0000 Subject: [Histonet] Tracking of blocks onto processors In-Reply-To: References: Message-ID: <7b477ea31d6148df995f2feb6ef7a75d@chla.usc.edu> Hi Chris, At our institution, we're currently utilizing CoPath Automatic Barcoding and Tracking (AB&T). Similar to Vantage, each step of the workload is tracked from accessioning ? grossing ? embedding? microtomy? staining? QC (or case assembly) and then delivery to pathologist. For every cassette that grossed by our PA's the day before, that information shows up on an automatically printed report for our techs to verify that the actual block exists the next day (when they are embedded and cut) or being accounted for at the time of filing. At 10 AM, a 2nd report will print highlighting any block that has yet to be cut (these blocks will either be in the grossed or embedded status). Usually, this final report is simply a blank page, because we typically have all of our routine workload cut before this time. If anything IS present on this report, it indicates that there is a cassette we didn't embed, have yet to cut, or worst case scenario, lost. This report is basically a red flag warning--it's a way to tell if anything is missing, and that you'd better locate that missing sample ASAP. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Willis, Donna G via Histonet Sent: Wednesday, March 22, 2023 5:29 AM To: Hagon, Christopher (Health) ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Tracking of blocks onto processors (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Chris, We have been tracking blocks since we went live with Vantage over 10 years ago. Roche (Ventana then) built a custom program for us to use at that time because they had not yet implemented it into Vantage. In the past 2 years Roche has updated their software to allow documenting this information. There are several options of how the scanning is performed. For our lab we scan before we place the block into the formalin holding tanks, right after the PA/Resident/Grossing Tech have placed the tissue into the block. I prefer it done there because I do not want my technicians exposed an extra time to formalin if the scanning is done right before the baskets are placed on the processors. We have a manual log that is used to document the time the cassette are placed in and taken out of the processors. We also track our blocks and slides into storage. I hope this helps. Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Hagon, Christopher (Health) via Histonet Sent: Tuesday, March 21, 2023 5:17 PM To: histonet at lists.utsouthwestern.edu Subject: {EXTERNAL} [Histonet] Tracking of blocks onto processors CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. UNOFFICIAL Hi Histonetters, Just wondering if or how others are tracking/scanning racks of blocks onto processors. In this day and age of traceability with barcodes, we scan each block in each rack before loading onto a processor and recording which processor that rack went on to. Has anyone got a more efficient way of doing this? Assigning at grossing/cut-up is an option, but is an extra step for the PA/registrar and relies on knowing which processor is in use that day. Looking in the crystal ball, will next gen processors automatically scan blocks as the first step? Load blocks, start the run and it produces a report of the blocks on that run? Interested to hear peoples thoughts. Cheers, Chris Hagon - Pathology Digital Solutions Division, ACT Health Directorate ----------------------------------------------------------------------- This email, and any attachments, may be confidential and also privileged. If you are not the intended recipient, please notify the sender and delete all copies of this transmission along with any attachments immediately. 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No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1MAasf5yHgchtAKL3_NaXwU6Cjez4wtqCwdBIuJEAr5jOGoaR0Yf9uAiawZKniIXgf6KQB6mpDo8k1SdlfkRdhuukA_3eryKbrhxN9p2-VHMcS0j2YyQD6NNt0wnCepG3OutpnoBeji18tXV0_o0qWuvxRNBINxX_vhDdT5JhsOcbUmga3K2se6aSXx9UjhxOgXugVbzcVGthAUcxDzcOIcYpPfkGnovs8Ybz7_5pG6LsDdghW76CjPHu3NzAUiir6LyOCveTb--1XrYwqQTtt0QOyU9NZyFnVNl1GVJHZg0ll3x6xhGEvHbI29xIkps-N6BCopDBuUR7LtZk1oX7MgJcStRhUsK0bIs4oWjK_UdJHy-6jwCc1Md0XHFyUX6oetSHExmrgk5SAod7cDsBaGVw6fyEuWo2J9DjrA_Xy0got4QpquyI5QPnEOxOoqI2gYY_bVXp7xdewVafkuWovQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From karen.heckford at commonspirit.org Wed Mar 22 12:45:25 2023 From: karen.heckford at commonspirit.org (Karen Heckford CA-San Francisco) Date: Wed, 22 Mar 2023 10:45:25 -0700 Subject: [Histonet] IHC gunk Message-ID: Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From Donna.Willis at BSWHealth.org Wed Mar 22 12:57:09 2023 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 22 Mar 2023 17:57:09 +0000 Subject: [Histonet] IHC gunk In-Reply-To: References: Message-ID: What instrumentation are you using. Have you talked to your vendor? Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 12:45 PM To: Histonet Subject: {EXTERNAL} [Histonet] IHC gunk CAUTION:??This email originated outside of BSWH. The actual sender is (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From tpodawiltz at yahoo.com Wed Mar 22 13:02:32 2023 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Wed, 22 Mar 2023 18:02:32 +0000 (UTC) Subject: [Histonet] IHC gunk In-Reply-To: References: Message-ID: <1412949951.2093261.1679508152947@mail.yahoo.com> Is it on all slides or just certain antibodies? Sent from Yahoo Mail for iPhone On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet wrote: What instrumentation are you using.? Have you talked to your vendor? Donna Willis Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave|Dallas, Texas 75246 214-820-2465 office|214-725-6184 mobile -----Original Message----- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 12:45 PM To: Histonet Subject: {EXTERNAL} [Histonet] IHC gunk CAUTION:??This email originated outside of BSWH. The actual sender is? (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon. Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue.? I have been told it is bacteria but the Pathologist and I kind of doubt that.? I cannot get a good picture of it to show.? It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system.? I have put fresh reagents on the instrument.? This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue.? I do not see this stuff elsewhere on the slide, only the tissue.? Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue.? I use DiH20 from the hospital system.? Not sure if this may be the problem.? I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution:? This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.? The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.? Any further review, dissemination, distribution, or copying of this message is strictly prohibited.? If you have received this communication in error, please notify us? immediately by reply email.? Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmacdonald at mtsac.edu Wed Mar 22 13:15:21 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Wed, 22 Mar 2023 18:15:21 +0000 Subject: [Histonet] IHC gunk In-Reply-To: References: Message-ID: Is it possible that it is hematoxylin precipitate? -----Original Message----- From: Karen Heckford CA-San Francisco via Histonet Sent: Wednesday, March 22, 2023 10:45 AM To: Histonet Subject: [Histonet] IHC gunk EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Good Morning, I have recently developed a problem with contamination of some kind on my IHC slides. The contamination is black clumps and lays on top of the tissue. I have been told it is bacteria but the Pathologist and I kind of doubt that. I cannot get a good picture of it to show. It may not be on all the slides on the same run or even the same antibody slide. I have decontaminated the whole system. I have put fresh reagents on the instrument. This stuff looks like it would wash off at the end of the run since it is sitting right on top of the tissue. I do not see this stuff elsewhere on the slide, only the tissue. Does not matter if the tissue was cut fresh or not. I have tried everything I can think of to get rid of it and still have this issue. I use DiH20 from the hospital system. Not sure if this may be the problem. I can have Engineering test the DiH20. Any help would be greatly appreciated. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-750-5751 karen.heckford@ commonspirit.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D&reserved=0 From edmartin26 at gmail.com Wed Mar 22 14:47:53 2023 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 22 Mar 2023 15:47:53 -0400 Subject: [Histonet] Histonet Digest, Vol 232, Issue 7 In-Reply-To: References: Message-ID: Hi KDean70 at hotmail.com, What likely is occurring but you didn?t mention in the thread is that your friend is using alkaline phosphatase based detection, which, by itself can react with kidney tissue. This likely explains why your friend is getting strong kidney staining and weak melan-a staining. This also tells me your friend didn?t optimize the antibody correctly. I?m not sure why your friend is choosing to use a lyophilized antibody for Melan-A as so many other commercially available options are available capable of refrigerated storage and good up to 3 years. CellMarque makes an Melan-A in both realty to use and/or a liquid concentrate with a 3-year expiry date. Best wishes, Eddie Martin, HTL,QIHC The National Institutes of Health Department of Laboratory Medicine Bone Marrow Service Eddie.martin at nih.gov (301)-594-2054 On Wed, Mar 15, 2023 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Problems with Melan A staining (Ken M) > > > > ---------- Forwarded message ---------- > From: Ken M > To: "histonet at lists.utsouthwestern.edu" > > Cc: > Bcc: > Date: Wed, 15 Mar 2023 16:51:03 +0000 > Subject: [Histonet] Problems with Melan A staining > One of my histotech friends is having issues with Melan A staining. Using > MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue > control was stained strongly positive while the positive melanoma tissue > control was stained weakly positive. He couldn?t figure out why after > several tries to reduce background. Can someone give me some suggestions? > Thank you very much! > > > Ken > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Thu Mar 23 13:53:44 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 23 Mar 2023 18:53:44 +0000 Subject: [Histonet] Tissue Processor Schedule Validations Message-ID: Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted. I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Bonnie.Whitaker at osumc.edu Thu Mar 23 14:02:18 2023 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Thu, 23 Mar 2023 19:02:18 +0000 Subject: [Histonet] Tissue Processor Schedule Validations In-Reply-To: References: Message-ID: Put in two cassettes of resection tissue that are cut to mimic biopsy sizes/tissue (colon resection for colon bx, liver resection for liver bx, etc. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Cooper, Brian via Histonet Sent: Thursday, March 23, 2023 2:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Schedule Validations Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted. I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!BNH5jKym3IGDv3-_KyumAjKnDWbxb7K-Mxub342zQuCVJaxevyK_aS2-XN6RzLBCaWNdS9n7UUwnWoFqslHDXBn-lpeM6iusmnKnQA$ From LNormington at uwhealth.org Thu Mar 23 14:05:14 2023 From: LNormington at uwhealth.org (Normington, Lacy) Date: Thu, 23 Mar 2023 19:05:14 +0000 Subject: [Histonet] Tissue Processor Schedule Validations In-Reply-To: References: Message-ID: We have purchased several disposable core biopsy instruments of varying gauges and take samples of large resection specimens. We also use the same grossing tools to take small samples of GI, endo, ecc, ect. Lacy -----Original Message----- From: Cooper, Brian via Histonet Sent: Thursday, March 23, 2023 1:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Schedule Validations WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted. I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CLnormington%40uwhealth.org%7C03f2d50b67804ea76a8a08db2bd01366%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638151944895780675%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=5tYyoAfrWZt2PYWHNSyOCN%2BVcSEIWTUWssKoyEKt0cA%3D&reserved=0 From tpodawiltz at yahoo.com Thu Mar 23 14:15:19 2023 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Thu, 23 Mar 2023 19:15:19 +0000 (UTC) Subject: [Histonet] Tissue Processor Schedule Validations In-Reply-To: References: Message-ID: <1943265672.2416754.1679598919494@mail.yahoo.com> I ordered small punch biopsy kits and made my own small biopsies from remnant tissue. Renal biopsy kits worked for making our own needle biopsies. Leica already has the protocols that work so part is easy.? Sent from Yahoo Mail for iPad On Thursday, March 23, 2023, 3:05 PM, Normington, Lacy via Histonet wrote: We have purchased several disposable core biopsy instruments of varying gauges and take samples of large resection specimens. We also use the same grossing tools to take small samples of GI, endo, ecc, ect. Lacy -----Original Message----- From: Cooper, Brian via Histonet Sent: Thursday, March 23, 2023 1:54 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Schedule Validations WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores).? Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted.? I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CLnormington%40uwhealth.org%7C03f2d50b67804ea76a8a08db2bd01366%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638151944895780675%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=5tYyoAfrWZt2PYWHNSyOCN%2BVcSEIWTUWssKoyEKt0cA%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Thu Mar 23 14:29:35 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Thu, 23 Mar 2023 19:29:35 +0000 (UTC) Subject: [Histonet] Tissue Processor Schedule Validations In-Reply-To: References: Message-ID: <1070230578.4433984.1679599775950@mail.yahoo.com> The one thing that bothers me about the preloaded programs is the paraffin temperature is set at 65 degrees C.? That's close to 10 degrees higher than the melting point of our paraffin. Does anyone know why the temp. is so high?? To me, a temp that high will cause problems with IHC and small biopsies.?? Has anyone changed it?? Or do folks go along with it just because it's already there?? I inherited that temp, to change it now would be pain since it is the one we use the most.? If I validated our current Peloris 3, I would have set the wax temperatures at 60 degrees. Sincerely, Paula Sicurello On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet wrote: Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores).? Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted.? I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message.? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bcooper at chla.usc.edu Thu Mar 23 15:07:56 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 23 Mar 2023 20:07:56 +0000 Subject: [Histonet] Tissue Processor Schedule Validations In-Reply-To: References: Message-ID: <008bbddf3e9a47b0a70af157d4cc3e8b@chla.usc.edu> WOW! I came back from lunch and I have seven responses already! Everyone said the same thing, which was my game plan, take punches from larger samples! Happy Friday Eve everyone! Thanks so much Histonet! Brian -----Original Message----- From: Cooper, Brian via Histonet Sent: Thursday, March 23, 2023 11:54 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Tissue Processor Schedule Validations (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good afternoon Histonet, We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores). Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted. I have a game plan in mind, but would love some additional input! How'd you do it? Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1s0j6zTa7ftENI3-Copl_OZVezZbNUokprof4LCe6q3Ieg8wp1KrjJE3SsSa2ZCuqQYUKjhcZzS1FjQvCxroLiu0dB3PABSpA3ALkb7uFq3YFNXUOp-_9osFkBHThqzucP3FeklqlqfOroJkgRp7ykITVlzFo_ddZk8MJTKZ0O-IeD6b8xrmonSmQQoubAC1eU2Y_gLfJOSJTJHG-uEFpDRSG2mNdW4XOKkPI5yQ_wqEeGeFlCwtDElXgbdHOv9YfI4pyvNBuTdUtK9kFIfASkYJaOi6B24YkHOTcVTJ3MQpn-Rmalr2Pw47qzd2fhx6oIpClo785MYP3gzK07bo7hIa0QUTa56jD8qUdmrxhDzKJzzshQWEZJOBne6DfS3OcDuOUpacaCwOtX4irP3yROMNql8i-5BMreTEWthpVvlQtDfrZOPuT32hrcIcop_dF4emh_YPYl8Ncm2Y4FfLsMg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From ewen.sutherland at bio-strategy.com Fri Mar 24 00:45:50 2023 From: ewen.sutherland at bio-strategy.com (Ewen Sutherland) Date: Fri, 24 Mar 2023 05:45:50 +0000 Subject: [Histonet] Histonet Digest, Vol 232, Issue 13 In-Reply-To: References: Message-ID: Re Gunk Check any of the seals or washers. When they breakdown they usually present with this sort of result on slides Ewen Sutherland Anatomical and Digital Product Specialist Bio-Strategy T: +61 3 9355 3900 / 1800 00 84 53 M: +61 417 460 019 ewen.sutherland at bio-strategy.com?? www.bio-strategy.com -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Friday, March 24, 2023 4:00 AM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 232, Issue 13 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://apc01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cewen.sutherland%40bio-strategy.com%7Cbb3a3ca388ef40c68f7e08db2bc01359%7Cdc481198eaba4892b6388f87a67e289c%7C0%7C0%7C638151876214863455%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=rZza%2FMrBdHrYYUy%2FHDCprDpqTGyFEe%2BZWtbPtOe%2BbYw%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From Nancy.Schmitt at mercyhealth.com Fri Mar 24 07:28:42 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 24 Mar 2023 12:28:42 +0000 Subject: [Histonet] BD Totalys - pap question Message-ID: Happy Friday! For those running Surepath paps on the BD Totalys - have you seen any "haloing" effect? It is not the hats, and when the service group makes adjustments the "halo" moves. Slides are fine to be read, we just know that this should not be. The service group has been great, I just though it was worth checking to see if anyone else had experienced this. Thoughts appreciated, Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor MercyOne Dubuque, IA Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From edmartin26 at gmail.com Fri Mar 24 09:48:18 2023 From: edmartin26 at gmail.com (Eddie Martin) Date: Fri, 24 Mar 2023 10:48:18 -0400 Subject: [Histonet] IHC gunk on slides In-Reply-To: References: Message-ID: Karen, Regardless of the instrument you?re using, if related to a reagent, it may be that your detection chromogen is breaking down and you need to report it, or you have a material that is prepared onsite, and used onboard the instrument, and unknowingly contaminated. it may help if you can provide an image. It can be done with a mobile phone up to the eyepiece if necessary. Best wishes, Eddie Martin The National Institutes of Health 301-594-2054 On Thu, Mar 23, 2023 at 1:00 PM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. IHC gunk (Karen Heckford CA-San Francisco) > 2. Re: IHC gunk (Willis, Donna G) > 3. Re: IHC gunk (Thomas Podawiltz) > 4. Re: IHC gunk (Mac Donald, Jennifer) > 5. Re: Histonet Digest, Vol 232, Issue 7 (Eddie Martin) > > > > ---------- Forwarded message ---------- > From: Karen Heckford CA-San Francisco > To: Histonet > Cc: > Bcc: > Date: Wed, 22 Mar 2023 10:45:25 -0700 > Subject: [Histonet] IHC gunk > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have this > issue. I use DiH20 from the hospital system. Not sure if this may be the > problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > > > > > ---------- Forwarded message ---------- > From: "Willis, Donna G" > To: Karen Heckford CA-San Francisco , > Histonet > Cc: > Bcc: > Date: Wed, 22 Mar 2023 17:57:09 +0000 > Subject: Re: [Histonet] IHC gunk > What instrumentation are you using. Have you talked to your vendor? > > Donna Willis > Anatomic Pathology Manager > Baylor Scott&White Health > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > > > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 12:45 PM > To: Histonet > Subject: {EXTERNAL} [Histonet] IHC gunk > > > CAUTION: This email originated outside of BSWH. The actual sender is ( > histonet-bounces at lists.utsouthwestern.edu) which may be different from > the display address in the From: field. Avoid action unless you know the > content is safe. Report suspicious emails using the PhishAlarm button > located in your Outlook ribbon. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ > > ********************************************************************** > The information contained in this e-mail may be privileged, confidential, > and/or protected from disclosure. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly prohibited and no waiver of any attorney-client, work product, or > other privilege is intended. No binding agreement on behalf of Baylor Scott > & White Health, or any affiliated entity, is permitted by e-mail without > express written confirmation by a duly authorized representative of Baylor > Scott & White Health. > > > > ---------- Forwarded message ---------- > From: Thomas Podawiltz > To: "Willis, Donna G" , Karen Heckford CA-San > Francisco , Histonet < > histonet at lists.utsouthwestern.edu> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 18:02:32 +0000 (UTC) > Subject: Re: [Histonet] IHC gunk > Is it on all slides or just certain antibodies? > > > Sent from Yahoo Mail for iPhone > > > On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > > What instrumentation are you using. Have you talked to your vendor? > > Donna Willis > Anatomic Pathology Manager > Baylor Scott&White Health > Baylor University Medical Center > 3500 Gaston Ave|Dallas, Texas 75246 > 214-820-2465 office|214-725-6184 mobile > > > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 12:45 PM > To: Histonet > Subject: {EXTERNAL} [Histonet] IHC gunk > > > CAUTION: This email originated outside of BSWH. The actual sender is ( > histonet-bounces at lists.utsouthwestern.edu) which may be different from > the display address in the From: field. Avoid action unless you know the > content is safe. Report suspicious emails using the PhishAlarm button > located in your Outlook ribbon. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ > > ********************************************************************** > The information contained in this e-mail may be privileged, confidential, > and/or protected from disclosure. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you are > not the intended recipient (or have received this e-mail in error) please > notify the sender immediately and destroy this e-mail. Any unauthorized > copying, disclosure or distribution of the material in this e-mail is > strictly prohibited and no waiver of any attorney-client, work product, or > other privilege is intended. No binding agreement on behalf of Baylor Scott > & White Health, or any affiliated entity, is permitted by e-mail without > express written confirmation by a duly authorized representative of Baylor > Scott & White Health. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ---------- Forwarded message ---------- > From: "Mac Donald, Jennifer" > To: Karen Heckford CA-San Francisco , > Histonet > Cc: > Bcc: > Date: Wed, 22 Mar 2023 18:15:21 +0000 > Subject: Re: [Histonet] IHC gunk > Is it possible that it is hematoxylin precipitate? > > -----Original Message----- > From: Karen Heckford CA-San Francisco via Histonet < > histonet at lists.utsouthwestern.edu> > Sent: Wednesday, March 22, 2023 10:45 AM > To: Histonet > Subject: [Histonet] IHC gunk > > EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > Good Morning, > I have recently developed a problem with contamination of some kind on my > IHC slides. > The contamination is black clumps and lays on top of the tissue. I have > been told it is bacteria but the Pathologist and I kind of doubt that. I > cannot get a good picture of it to show. It may not be on all the slides > on the same run or even the same antibody slide. > > I have decontaminated the whole system. I have put fresh reagents on the > instrument. This stuff looks like it would wash off at the end of the run > since it is sitting right on top of the tissue. I do not see this stuff > elsewhere on the slide, only the tissue. Does not matter if the tissue > was cut fresh or not. > > I have tried everything I can think of to get rid of it and still have > this issue. I use DiH20 from the hospital system. Not sure if this may be > the problem. I can have Engineering test the DiH20. > > Any help would be greatly appreciated. > > Thanks, > > Karen Heckford HT ASCP CE > > Lead Histology Technician > > St. Mary's Medical Center > > 450 Stanyan St. > > San Francisco, Ca. 94117 > > 415-750-5751 > > karen.heckford@ commonspirit.org > > > > > Caution: This email message, including all content and attachments, is > CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The > information contained in this email message is intended only for the use of > the recipient(s) named above. If the reader of this message is not the > intended recipient or an agent responsible for delivering it to the > intended recipient, you have received this document in error. Any further > review, dissemination, distribution, or copying of this message is strictly > prohibited. If you have received this communication in error, please > notify us immediately by reply email. Thank you > > Caution: This email is both proprietary and confidential, and not intended > for transmission to (or receipt by) any unauthorized person(s). If you > believe that you have received this email in error, do not read any > attachments. Instead, kindly reply to the sender stating that you have > received the message in error. Then destroy it and any attachments. Thank > you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D&reserved=0 > > > > > > ---------- Forwarded message ---------- > From: Eddie Martin > To: histonet at lists.utsouthwestern.edu, "kdean70 at hotmail.com" < > kdean70 at hotmail.com> > Cc: > Bcc: > Date: Wed, 22 Mar 2023 15:47:53 -0400 > Subject: Re: [Histonet] Histonet Digest, Vol 232, Issue 7 > Hi KDean70 at hotmail.com, > > What likely is occurring but you didn?t mention in the thread is that your > friend is using alkaline phosphatase based detection, which, by itself can > react with kidney tissue. This likely explains why your friend is getting > strong kidney staining and weak melan-a staining. > > This also tells me your friend didn?t optimize the antibody correctly. I?m > not sure why your friend is choosing to use a lyophilized antibody for > Melan-A as so many other commercially available options are available > capable of refrigerated storage and good up to 3 years. CellMarque makes an > Melan-A in both realty to use and/or a liquid concentrate with a 3-year > expiry date. > > Best wishes, > Eddie Martin, HTL,QIHC > The National Institutes of Health > Department of Laboratory Medicine > Bone Marrow Service > Eddie.martin at nih.gov > (301)-594-2054 > > > > On Wed, Mar 15, 2023 at 1:00 PM > > wrote: > > > Send Histonet mailing list submissions to > > histonet at lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request at lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner at lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > Today's Topics: > > > > 1. Problems with Melan A staining (Ken M) > > > > > > > > ---------- Forwarded message ---------- > > From: Ken M > > To: "histonet at lists.utsouthwestern.edu" < > histonet at lists.utsouthwestern.edu > > > > > Cc: > > Bcc: > > Date: Wed, 15 Mar 2023 16:51:03 +0000 > > Subject: [Histonet] Problems with Melan A staining > > One of my histotech friends is having issues with Melan A staining. Using > > MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue > > control was stained strongly positive while the positive melanoma tissue > > control was stained weakly positive. He couldn?t figure out why after > > several tries to reduce background. Can someone give me some suggestions? > > Thank you very much! > > > > > > Ken > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet at lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Fri Mar 24 10:53:25 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Fri, 24 Mar 2023 11:53:25 -0400 Subject: [Histonet] The One Question You Hate to Answer, How to Answer It and Some Exciting NEW Job Opportunities with bonuses up to 15K! Message-ID: <005d01d95e68$c69f5200$53ddf600$@earthlink.net> The One Question You Hate to Answer and How to Answer It. And Exciting Histology Job opportunities You Don't Want to Miss Out On!! Hi Histopeeps, I hope you are doing well and having a wonderful week. I found this article online and HAD to share it. ? This is great advice for doing any type of job or promotion interview! ? You know the dreaded question! ? The on that could make or break you no matter what! ? This question: What do you like least about your current position? Here?s your answer: How to answer the Question What do you like least about your job? https://www.themuse.com/.../what-do-you-like-least-about...? Histopeeps, I also want to update you on the Traveler Study. I am wrapping up my research and getting ready to write. I expect to have it out sometime in April. In the meantime, if you or any travelers you know would like to share some anecdotal experiences or insights about travel I welcome the input!! AND if you want to read the article be sure and SUBSCRIBE to my histology careers bulletin at relia1 at earthlink.net Check out the exciting opportunities! RELIA?s Current Opportunities! ? Check out my newest opportunities in Oxford MS. Johnson City, TN, and Cheyenne, WY. ? And my exciting opportunities to learn and grow in brand new labs and more! ? And don?t forget excellent compensation, benefits, Relocation and Sign On bonuses! ? With opportunities located in MS, IA, WY, PA, OH, FL, CA, VA and TN! ? All permanent full-time positions. ? My clients are willing to wait until your contract ends! ? You won?t have to give up vacations or take your kids out of school. ? My clients are willing to work within YOUR timeframes!! ? My clients are excited to meet you and show you how they can help your career! ? My clients want to show you what they have to offer you! ? Do MORE than just CUT and EMBED!! Leadership: MS ? Oxford: Pathology; Histology Supervisor IA ? Des Moines: Dermpath; Supervisor Brand new lab! WY ? Cheyenne: GI; 1 person lab! PA ? Harrisburg: CancerDX; 1 person lab! FL ? Sarasota: GI; Histology Supervisor FL ? Fort Myers: CancerDX; Quality Assurance CA ? Bakersfield: Pathology; Histology Manager CA ? Aliso Viejo: Cancer DX; Quality Assurance HT/HTL and Eligible: IA ? Des Moines: Dermpath PA ? Harrisburg: CancerDX; 1 person lab! WY ? Cheyenne, GI 1 person lab! VA ? Virginia Beach 15K sign on! FL ? Bradenton: GI FL ? Fort Myers: CancerDX OH ? Dayton: Dermpath TN ? Johnson City!! Smoky Mountains!! TN ? Nashville: General Pathology Remember if you or someone you know is looking for an opportunity in another area let me do the leg work for you!! I really appreciate you taking the time to read this post and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached toll free at 866-607-3542 866-60RELIA Text or call me on my cell at 407-353-5070. Or email me at: relia1 at earthlink.net I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions #jobs4myhistopeeps #ilovemyhistopeeps #hmuhistopeeps #histology #histologyiscool #histologyjobs #histologycareers From gu.lang at gmx.at Tue Mar 28 09:30:30 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Tue, 28 Mar 2023 16:30:30 +0200 Subject: [Histonet] Sudanblack B on FFPET Message-ID: <000c01d96181$d9e6b030$8db41090$@gmx.at> Hallo! Has anyone experience with Sudanblack B on paraffin slides for staining lipofuszin? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. I've made a saturated Sudanblack B-solution in 70% ethanol and tried it with 10 min on liver without real success. I would appreciate any input and help. Thanks in advance Gudrun Lang Austria From jkiernan at uwo.ca Tue Mar 28 12:06:25 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 28 Mar 2023 17:06:25 +0000 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: <000c01d96181$d9e6b030$8db41090$@gmx.at> References: <000c01d96181$d9e6b030$8db41090$@gmx.at> Message-ID: You probably would get better results with a supersaturated solution of the dye in 60% isopropanol with added dextrin. For details see https://www.urmc.rochester.edu/urmc-labs/pathology/StainsManual/. Click on Table of Contents and follow the links to fined the method. Be sure to use a batch of Sudan black B certified by the Biological Stain Commission. John Kiernan London, Canada = = = ________________________________ From: Gudrun Lang via Histonet Sent: March 28, 2023 10:30 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Sudanblack B on FFPET Hallo! Has anyone experience with Sudanblack B on paraffin slides for staining lipofuszin? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. I've made a saturated Sudanblack B-solution in 70% ethanol and tried it with 10 min on liver without real success. I would appreciate any input and help. Thanks in advance Gudrun Lang Austria _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Tue Mar 28 12:45:46 2023 From: rsrichmond at gmail.com (Bob Richmond) Date: Tue, 28 Mar 2023 13:45:46 -0400 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: References: Message-ID: > > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. - I've made a saturated Sudan black B-solution in 70% ethanol and tried it with10 min on liver without real success.<< The main thing you need to do is demonstrate that it isn't hemosiderin with an iron stain (Perls prussian blue reaction), and perhaps also that it isn't acid-fast. Lipofuscin can be identified an H & E staining, except for these considerations. Bob Richmond Maryville, Tennessee From relia1 at earthlink.net Tue Mar 28 13:35:35 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 28 Mar 2023 14:35:35 -0400 Subject: [Histonet] The Florida Society of Histotechnology invites you to their Annual Meeting May 6th 2023 at the Keiser University Campus in Orlando, FL Message-ID: <000201d961a4$168ed310$43ac7930$@earthlink.net> Hi Histopeeps! Hope to see you there! FSH 2023 Annual Meeting WHEN: May 06, 2023 7:45 AM - 5:00 PM Please join us for a day of education, fun, food, seeing old friends and meeting new ones! Keiser University has opened its doors to FSH to hold their much-awaited annual state meeting and we cannot wait to see you there! ? Make a day of it and bring your whole lab! ? Earn 6 credit hours, enjoy a free lunch, ? And spend time getting acquainted with our vendors who are providing gifts for our raffle! REGISTER AT: https://fshgroup.org TICKETS: $75.00 Member Registration $110.00 Non-Member registration $90.00 Non Member Registration with membership $25.00 Licensed Student Registration WHERE: 5600 Lake Underhill Road Orlando, FL 32807 Thanks-Pam RELIA Solutions Email: relia1 at earthlink,net Cell/text: 407-353-5070 Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From afhenwood at outlook.com Tue Mar 28 14:13:16 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Tue, 28 Mar 2023 19:13:16 +0000 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: References: Message-ID: I would also let the saturated solution stand for a few days. Like Oil Red O, it probably needs time to ?mature?. I would also use a frozen section of skin as a control. Regards, Tony Henwood Sydney, Australia From: Bob Richmond via Histonet Sent: Wednesday, 29 March 2023 4:51 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sudanblack B on FFPET > > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. - I've made a saturated Sudan black B-solution in 70% ethanol and tried it with10 min on liver without real success.<< The main thing you need to do is demonstrate that it isn't hemosiderin with an iron stain (Perls prussian blue reaction), and perhaps also that it isn't acid-fast. Lipofuscin can be identified an H & E staining, except for these considerations. Bob Richmond Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jobaluyut at gmail.com Tue Mar 28 15:12:31 2023 From: jobaluyut at gmail.com (AJ Cabral) Date: Tue, 28 Mar 2023 13:12:31 -0700 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: References: Message-ID: Sudan Black staining won?t work on FFPET. The alcohols and xylenes used in the tissue processing dissolves the lipids in the tissue. However, you can used formalin fixed tissue as an alternative if no frozen section is available. Rinse the tissue in distilled water for several minutes, pat dry, freeze the tissue on OCT, cut frozen sections and stain in Sudan black. Have you considered looking into acid phosphatase staining for lipofucshin? It is non specific but it can be demonstrated in muscle biopsy. Cheers, Joanna On Tue, Mar 28, 2023 at 12:22 PM Tony Henwood via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I would also let the saturated solution stand for a few days. Like Oil Red > O, it probably needs time to ?mature?. I would also use a frozen section of > skin as a control. > > Regards, > > Tony Henwood > Sydney, Australia > > From: Bob Richmond via Histonet > Sent: Wednesday, 29 March 2023 4:51 AM > To: Histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] Sudanblack B on FFPET > > > > > Gudrun Lang in Austria asks: > > > > >>Has anyone experience with Sudanblack B on paraffin slides for staining > [lipofuscin]? A doctor wants the demonstration of the lipoid content of > foamy cells or granulocytes in lung. I've found protocols that have > incubation-times from 10 minutes to over-night. - I've made a saturated > Sudan black B-solution in 70% ethanol and tried it with10 min on liver > without real success.<< > > The main thing you need to do is demonstrate that it isn't hemosiderin with > an iron stain (Perls prussian blue reaction), and perhaps also that it > isn't acid-fast. Lipofuscin can be identified an H & E staining, except for > these considerations. > > Bob Richmond > Maryville, Tennessee > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jkiernan at uwo.ca Tue Mar 28 23:13:05 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Wed, 29 Mar 2023 04:13:05 +0000 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: References: Message-ID: It's true that Sudan black B won't stain ordinary lipids (fat, phospholipids etc) that are absent from paraffin sections. The lipid in lipofuscin is bound to protein strongly enough to resist extraction during passage through all the solvents used in preparing paraffin sections. Churukian's adaptation of the Lillie & Ashburn method (link to a free download methods book in my recent Histonet email) is OK. It is one of the Biological Stain Commission's tests for certifying Sudan black B. John Kiernan London, Ontario = = = ________________________________ From: AJ Cabral via Histonet Sent: March 28, 2023 4:12 PM To: Tony Henwood Cc: Histonet at lists.utsouthwestern.edu ; Bob Richmond Subject: Re: [Histonet] Sudanblack B on FFPET Sudan Black staining won?t work on FFPET. The alcohols and xylenes used in the tissue processing dissolves the lipids in the tissue. However, you can used formalin fixed tissue as an alternative if no frozen section is available. Rinse the tissue in distilled water for several minutes, pat dry, freeze the tissue on OCT, cut frozen sections and stain in Sudan black. Have you considered looking into acid phosphatase staining for lipofucshin? It is non specific but it can be demonstrated in muscle biopsy. Cheers, Joanna On Tue, Mar 28, 2023 at 12:22 PM Tony Henwood via Histonet < histonet at lists.utsouthwestern.edu> wrote: > I would also let the saturated solution stand for a few days. Like Oil Red > O, it probably needs time to ?mature?. I would also use a frozen section of > skin as a control. > > Regards, > > Tony Henwood > Sydney, Australia > > From: Bob Richmond via Histonet > Sent: Wednesday, 29 March 2023 4:51 AM > To: Histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu> > Subject: Re: [Histonet] Sudanblack B on FFPET > > > > > Gudrun Lang in Austria asks: > > > > >>Has anyone experience with Sudanblack B on paraffin slides for staining > [lipofuscin]? A doctor wants the demonstration of the lipoid content of > foamy cells or granulocytes in lung. I've found protocols that have > incubation-times from 10 minutes to over-night. - I've made a saturated > Sudan black B-solution in 70% ethanol and tried it with10 min on liver > without real success.<< > > The main thing you need to do is demonstrate that it isn't hemosiderin with > an iron stain (Perls prussian blue reaction), and perhaps also that it > isn't acid-fast. Lipofuscin can be identified an H & E staining, except for > these considerations. > > Bob Richmond > Maryville, Tennessee > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Mar 29 11:01:49 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 29 Mar 2023 12:01:49 -0400 Subject: [Histonet] Kitchen Wars, March Madness and Exciting opportunities with bonuses up to 15K Most are RELIA EXCLUSIVES!! Message-ID: <01ab01d96257$c6812710$53837530$@earthlink.net> Hi Histopeeps! I hope you are having a great week! March Madness is ON and in addition to some great College Basketball. I have been experiencing the madness as well. I found this awesome article online great for sharing "around the water cooler" ICYMI: Kitchen Wars are back! Office drama advice: The kitchen wars are back. Here's a creative solution. (slate.com) And... My phone has literally been ringing off the hook with some great new opportunities. All of these positions are permanent full-time positions, and my clients offer excellent compensation, benefits and relocation assistance and or sign on bonuses up to 15K. Best of all these clients are motivated to hire and eager to meet you! Check out the exciting opportunities! RELIA's Current Opportunties! *Check out my newest opportunities in Oxford MS. Johnson City, TN, and Cheyenne, WY. *And my exciting opportunities to learn and grow in brand new labs and more! *And don't forget excellent compensation, benefits, and Relocation and Sign On bonuses! *With opportunities located in MS, IA, WY, PA, OH, FL, CA, VA and TN! *All permanent full-time positions. *My clients are willing to wait until your contract ends! *You won't have to give up vacations or take your kids out of school. *My clients are willing to work within YOUR timeframes!! *My clients are excited to meet you and show you how they can help your career! *My clients want to show you what they have to offer you! *Do MORE than just CUT and EMBED!! Leadership: *MS - Oxford: Pathology; Histology Supervisor *IA - Des Moines: Dermpath; Supervisor Brand new lab! *WY - Cheyenne: GI; 1 person lab! *PA - Harrisburg: CancerDX; 1 person lab! *FL - Sarasota: GI; Histology Supervisor *FL - Fort Myers: CancerDX; Quality Assurance *CA - Bakersfield: Pathology; Histology Manager *CA - Aliso Viejo: Cancer DX; Quality Assurance HT/HTL and Eligible: *IA - Des Moines: Dermpath *PA - Harrisburg: CancerDX; 1 person lab! *WY - Cheyenne, GI 1 person lab! *VA - Virginia Beach 15K sign on! *FL - Bradenton: GI *FL - Fort Myers: CancerDX *OH - Dayton: Dermpath *TN - Johnson City!! Smoky Mountains!! *TN - Nashville: General Pathology Remember if you or someone you know is looking for an opportunity in another area let me do the leg work for you!! I really appreciate you taking the time to read this post and it means a lot to me when you take the time to refer your friends and coworkers so to show my appreciation I would like to offer you a referral fee for anyone you refer to me that I place. So, if you think you or someone you know might be interested please contact me. >I can be reached toll free at 866-607-3542 866-60RELIA >Text or call me on my cell at 407-353-5070. >Or email me at: relia1 at earthlink.net I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #jobs4myhistopeeps #ilovemyhistopeeps #hmuhistopeeps #histology #histologyiscool #histologyjobs #histologycareers From gu.lang at gmx.at Wed Mar 29 11:12:16 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Wed, 29 Mar 2023 18:12:16 +0200 Subject: [Histonet] Sudanblack B on FFPET In-Reply-To: References: Message-ID: <002e01d96259$3b4688f0$b1d39ad0$@gmx.at> Many thanks to all for your helpful hints. Kind regards Gudrun Lang -----Urspr?ngliche Nachricht----- Von: Tony Henwood via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Dienstag, 28. M?rz 2023 21:13 An: Bob Richmond; Histonet at lists.utsouthwestern.edu Betreff: Re: [Histonet] Sudanblack B on FFPET I would also let the saturated solution stand for a few days. Like Oil Red O, it probably needs time to ?mature?. I would also use a frozen section of skin as a control. Regards, Tony Henwood Sydney, Australia From: Bob Richmond via Histonet Sent: Wednesday, 29 March 2023 4:51 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Sudanblack B on FFPET > > Gudrun Lang in Austria asks: > >>Has anyone experience with Sudanblack B on paraffin slides for staining [lipofuscin]? A doctor wants the demonstration of the lipoid content of foamy cells or granulocytes in lung. I've found protocols that have incubation-times from 10 minutes to over-night. - I've made a saturated Sudan black B-solution in 70% ethanol and tried it with10 min on liver without real success.<< The main thing you need to do is demonstrate that it isn't hemosiderin with an iron stain (Perls prussian blue reaction), and perhaps also that it isn't acid-fast. Lipofuscin can be identified an H & E staining, except for these considerations. Bob Richmond Maryville, Tennessee _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Mar 29 11:13:24 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 29 Mar 2023 12:13:24 -0400 Subject: [Histonet] Here is the link to the article on Kitchen Wars. Message-ID: <01d101d96259$64a42270$2dec6750$@earthlink.net> ICYMI: Kitchen Wars are back! Office drama advice: The kitchen wars are back. Here 's a creative solution. (slate.com) Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From PKRichar at gundersenhealth.org Wed Mar 29 12:55:44 2023 From: PKRichar at gundersenhealth.org (Richardson, Pam K) Date: Wed, 29 Mar 2023 17:55:44 +0000 Subject: [Histonet] Grossing station Cassette shelf In-Reply-To: References: Message-ID: Does anyone know where I can purchase a shelf that would attach to Mopec grossing stations? I have a picture of one but cannot find one to order. Best Wishes Pam ~ National Histology Professionals Day 3/10/2023 Pathologists' Assistant Day 4/14/2023 Administrative Professionals Day 4/26/2023 Medical Laboratory Professionals Week April 23-29, 2023 National Cytotechnology Day 5/13/2023 Microbiology Day 6/27/2023 +++++++++++++++++++++++++++ Pam Richardson Clinical Manager Gundersen Health System Laboratory Services Email: pkrichar at gundersenhealth.org Phone: 608 775-4133 Fax: 608 775-6136 Interdepartmental Mail Stop: H04-007 E-visit us at: http://www.gundersenhealth.org From Christopher.Conlisk at ucsf.edu Thu Mar 30 12:49:32 2023 From: Christopher.Conlisk at ucsf.edu (Conlisk, Christopher) Date: Thu, 30 Mar 2023 17:49:32 +0000 Subject: [Histonet] Open positions HTI, HTII Message-ID: We have two great positions opened Job Posting Preview Histotechnologist I Pathology - Surgical Histology Full Time 72122BR Job Summary Under supervision by Senior-level technologists, Lead technologist and the Histology Supervisor, the incumbent serves as a Histotechnologist in the Histology laboratory. Duties include tissue processing, embedding, paraffin sectioning, H&E staining, Special Staining, specimen receipt and accessioning, Laboratory Information System operation, Quality Assurance record keeping, instrument maintenance and other technical duties as assigned, including coverage in the Immunohistochemistry laboratory . Rotates weekly between workstations within the lab. Work schedule is variable to include Weekends and holiday coverage as scheduled. Incumbent must be able to flex work hours as needed to meet Department operational needs and cover work rotations. The final salary and offer components are subject to additional approvals based on UC policy. To see the salary range for this position (we recommend that you make a note of the job code and use that to look up): TCS Non-Academic Titles Search (ucop.edu) Please note: The compensation ranges listed online for roles not covered by a bargaining unit agreement are very wide, however a job offer will typically fall in the range of 80% - 120% of the established mid-point. An offer will take into consideration the experience of the final candidate AND the current salary level of individuals working at UCSF in a similar role. For roles covered by a bargaining unit agreement, there will be specific rules about where a new hire would be placed on the range. To learn more about the benefits of working at UCSF, including total compensation, please visit: https://ucnet.universityofcalifornia.edu/compensation-and-benefits/index.html Required Qualifications Associate degree or at least 60 semester hours of academic credit from a regionally accredited college/university, with a combination of 12 semester hours of biology and chemistry, AND one year of experience as a histotechnologist, preferably in a high-volume hospital histology laboratory within the last five years, or completion of an associate degree from an accredited Histotechnology program. Excellent interpersonal and communication skills required. Preferred Qualifications * One to two years experience as a Histotechnologist * ASCP certification: HT or HTL licensed strongly preferred About UCSF At UCSF Health, our mission of innovative patient care, advanced technology and pioneering research is redefining what's possible for the patients we serve - a promise we share with the professionals who make up our team. Consistently ranked among the top 10 hospitals nationwide by U.S. News & World Report - UCSF Health is committed to providing the most rewarding work experience while delivering the best care available anywhere. In an environment that allows for continuous learning and opportunities for professional growth, UCSF Health offers the ideal atmosphere in which to best use your skills and talents. Pride Values UCSF is a diverse community made of people with many skills and talents. We seek candidates whose work experience or community service has prepared them to contribute to our commitment to professionalism, respect, integrity, diversity and excellence - also known as our PRIDE values. In addition to our PRIDE values, UCSF is committed to equity - both in how we deliver care as well as our workforce. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care. Additional information about UCSF is available at diversity.ucsf.edu Join us to find a rewarding career contributing to improving healthcare worldwide. Equal Employment Opportunity The University of California San Francisco is an Equal Opportunity/Affirmative Action Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, protected veteran or disabled status, or genetic information. Organization Health Job Code and Payroll Title 009065 HISTO TCHNO 1 Job Category Allied Health, Clinical Labs Bargaining Unit American Federation of State, County and Municipal Employees - Patient Care Technical Unit (AFSCME-EX) Employee Class Career Percentage 100% Location Mount Zion (SF) Shift Days, Evenings, Nights, Weekends, Variable Shift Length 8 Hours Additional Shift Details Sunday-Thursday 10:30PM-7:00AM Christopher Conlisk, HT(ASCP), PBT(ASCP) Histology Department Supervisor Anatomic Pathology University of California, San Francisco 1600 Divisadero Street, Room B217 Phone: 415-514-6042 Cell: 415-407-5303 CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. From Nancy.Schmitt at mercyhealth.com Fri Mar 31 05:26:41 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Fri, 31 Mar 2023 10:26:41 +0000 Subject: [Histonet] Mohs cryostat Message-ID: Hello and Happy Friday! Any thoughts on Epredia vs Leica for cryostat? This will be used for Mohs - I have only used Leica for frozen sectioning and appreciate any advice. Thanks! Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.