From Valerie.Hannen at parrishmed.com Thu Jun 1 14:40:24 2023 From: Valerie.Hannen at parrishmed.com (Hannen, Valerie) Date: Thu, 1 Jun 2023 19:40:24 +0000 Subject: [Histonet] Coffee at the desk Message-ID: Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area ( meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk?? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com From tpodawiltz at yahoo.com Thu Jun 1 15:00:30 2023 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Thu, 1 Jun 2023 20:00:30 +0000 (UTC) Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: <162780967.2965690.1685649630442@mail.yahoo.com> OSHA: bloodborne pathogen standard.A clean area is an area that is closed off from the working environment with walls and door. Tape is not a appropriate alternative, maybe on WKRP in Cincinnati, but not in a lab.? On Thursday, June 1, 2023 at 03:40:41 PM EDT, Hannen, Valerie via Histonet wrote: Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area (? meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk??? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333? Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com www.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet | | Virus-free.www.avast.com | From smclaughlin7 at cooleydickinson.org Fri Jun 2 05:09:41 2023 From: smclaughlin7 at cooleydickinson.org (McLaughlin, Stacy L.) Date: Fri, 2 Jun 2023 10:09:41 +0000 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: It is not allowed. The area you want food/drink has to be completely separate- floor to ceiling walls and a door. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughlin7 at cooleydickinson.org -----Original Message----- From: Hannen, Valerie via Histonet Sent: Thursday, June 1, 2023 3:40 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Coffee at the desk External Email - Use Caution Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area ( meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk?? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com http://secure-web.cisco.com/1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q/http%3A%2F%2Fwww.parrishmed.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Mass General Brigham Compliance HelpLine at https://www.massgeneralbrigham.org/complianceline . Please note that this e-mail is not secure (encrypted). If you do not wish to continue communication over unencrypted e-mail, please notify the sender of this message immediately. Continuing to send or respond to e-mail after receiving this message means you understand and accept this risk and wish to continue to communicate over unencrypted e-mail. From Jessica.Piche at wtbyhosp.org Fri Jun 2 07:36:52 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Fri, 2 Jun 2023 12:36:52 +0000 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: It also doesn't set a good impression for everyone else in the lab since they can't have a coffee at their stations. If it could be done, I wouldn't want everyone else's coffees on my desk either, but I'd have to because I'd want to make it fair to everyone. If only it was safe for all of us.... Have a great weekend everyone! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: McLaughlin, Stacy L. via Histonet Sent: Friday, June 2, 2023 6:09 AM To: Hannen, Valerie ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coffee at the desk [EXTERNAL MSG] It is not allowed. The area you want food/drink has to be completely separate- floor to ceiling walls and a door. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughlin7 at cooleydickinson.org -----Original Message----- From: Hannen, Valerie via Histonet Sent: Thursday, June 1, 2023 3:40 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Coffee at the desk External Email - Use Caution Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area ( meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk?? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=j200BRfpFcuOoSsxlA72ykoKjkqRHJDLCC7ISOWAuNE%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailman%252Flistinfo%252Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=q9q1npoiwckRyrsVmqjSJu7zA2wsQGQSoNDGiBxYZtI%3D&reserved=0 The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Mass General Brigham Compliance HelpLine at https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.massgeneralbrigham.org%2Fcomplianceline&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=FTM1qMH8yaSCp52d91TXceTwZhGHrd%2BFVUCLvaLccyc%3D&reserved=0 > . Please note that this e-mail is not secure (encrypted). If you do not wish to continue communication over unencrypted e-mail, please notify the sender of this message immediately. Continuing to send or respond to e-mail after receiving this message means you understand and accept this risk and wish to continue to communicate over unencrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0s4%2FqlgUO580fR5tpdGAJJDWE3%2FTSUw2q%2Fh2rfawKT4%3D&reserved=0 From melissa at alliedsearchpartners.com Fri Jun 2 10:38:20 2023 From: melissa at alliedsearchpartners.com ('Melissa Owens') Date: Fri, 02 Jun 2023 15:38:20 +0000 Subject: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need Message-ID: <20230602153820.b4a91a6022ad1016@alliedsearchpartners.com> li {display:list-item;text-indent: -1em;} ul, ol{margin-left: 40px !important; padding-left: 0px !important;} When workloads spike, unexpected projects hit, employees are out for PTO, Sick or Parental Leave, we got your Lab Covered! Our Staffing Services for the lab can get the work done quickly. We have skilled laboratory talent at the ready and range of temporary staffing options to meet your unique needs. ? Short Term/Leave Coverage ? Last Minute Sick Coverage ? Long Term Leave Coverage ? Long Term Laboratory Training Program ? Contract to Hire (Asses a candidate on the job before committing to hiring them for a permanent role) We place skilled laboratory talent across the Anatomic and Clinical Pathology specializations. ? No minimum commitment on number of weeks you need laboratory staffing (Customized to your needs) ? For Sick Coverage there is 6 hour/day Minimum hours billed ? All inclusive bill rates (Travel/Pay Rate/Employment Taxes, etc.) Candidates we have right now who can make an impact in your lab! Histotech Supervisor/ Manager (2 Available) * Certified Histotech (ASCP) with over 20 years of experience * Seeking Temporary or Permanent work * Location: Seeking Anywhere along the East coast from FL to ME, West Coast * Available: Now Histotech * ASCP Certified with 10 years of experience * Primarily looking for Permanent but would also consider Temp/Travel work * Georgia * Available: Starting in July 2023 Histotech (5 Available Candidates) * ASCP Certified and 5 years of experience * Looking for Travel/Temp Position but open for Temp-Permanent (North Carolina) * Locations of interest: Anywhere in the US, Des Moines, IA, South Charleston, SC, Birmingham, AL * Available: Now Cytotechnologist-(1 Candidate Available) * Several years of experience with ASCP certification and Imaging Trained * Seeking primarily Temporary or Temporary to Permanent * Locations of interest: Anywhere in US (Not State Licensed) * Available: Now Medical Technologist-(7 Available Candidates) * Several years? experience as a Generalists * Seeking Temporary/Travel * Locations of Interest: Anywhere in US * Availability: Now Medical Laboratory Technician-Generalist-(3 Available Candidates) * Has 2 years? experience and MLT Certification * Seeking Permanent or Temporary Work * Location seeking: Houston, TX area * Available: Now Molecular Technologist ? (3 Candidates Available) * ASCP Molecular Specialists with 2+ years of experience * Seeking Permanent, Temp to Permanent or Permanent * Location: Anywhere in US * Available: Now Phlebotomist- (2 Available Candidates) * Certified Phlebotomist (CPT) and ASCP Certified Phlebotomists and 5-10 years of experience * Seeking Temp/Travel Work Only * Location Seeking: Anywhere in US * Ability to Train other Phlebotomists: YES * Available: Now Medical Assistant with Phlebotomy Experience- (2 Candidates Available) * Has Medical Assistant Diploma with phlebotomy experience and over 3 years? experience * Seeking Temporary or Permanent work * Location seeking: Houston, TX * Available: April *If you do not see a professional on this list that fits with what your talent needs are please inquire directly to discuss our pipeline in your area and for your position.* Ask me about our Laboratory Training Program, Government Services, Temporary or Permanent Staffing options! I'd love to connect. Here's my calendar link ( https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fcalendly.com%2Fmelissaowens ) to make finding time easy. Melissa Owens, CHP-ASA Allied Search Partners AN MRINETWORK MEMBER www.alliedsearchpartners.com ( https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=http%3A%2F%2Fwww.alliedsearchpartners.com ) Direct (Call) Line: 386 339 0839 li {display:list-item;text-indent: -1em;} ul, ol{margin-left: 40px !important; padding-left: 0px !important;} Click here ( https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fapp.loxo.co%2Fagencies%2F13989%2Funsubscribe%2FadaTfk64aUjeYmZ8 ) to unsubscribe From jaylundgren at gmail.com Fri Jun 2 12:15:27 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 2 Jun 2023 12:15:27 -0500 Subject: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need In-Reply-To: <20230602153820.b4a91a6022ad1016@alliedsearchpartners.com> References: <20230602153820.b4a91a6022ad1016@alliedsearchpartners.com> Message-ID: As far as I know, Histonet doesn't really allow advertising for individuals or agencies *looking* for jobs. There are a very few, selective, agencies that are allowed to post on Histonet tastefully and infrequently, but they are buying, not selling. Or histotechs who let others know about open positions in their organization. That's cool. I'm not a moderator, but this is supposed to be a collegial forum for practical histopathology advice. There are plenty of job search sites out there already. But if Histonet does start allowing this, then ***%%%$$$HEY SCRIPPS INSTITUTE HIRE ME!!!!$$$%%%%*** ***%%%$$$LONG OR SHORT CONTRACT!!!CHEAP$$$%%%*** **%%%$$$BIG TIME IMUNNOHISTOCHEMISTRY!!!!$$$%%%*** ***%%%$$$USAF HTL (ASCP) M.S. 30+ YRS!!!!!!!!!!!$$$%%%*** Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 2, 2023 at 10:50?AM 'Melissa Owens' via Histonet < histonet at lists.utsouthwestern.edu> wrote: > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > When workloads spike, unexpected projects hit, employees are out > for PTO, Sick or Parental Leave, we got your Lab Covered! Our > Staffing Services for the lab can get the work done quickly. We > have skilled laboratory talent at the ready and range of > temporary staffing options to meet your unique needs. > > ? Short Term/Leave Coverage > > ? Last Minute Sick Coverage > > ? Long Term Leave Coverage > > ? Long Term Laboratory Training Program > > ? Contract to Hire (Asses a candidate on the job before > committing to hiring them for a permanent role) > > We place skilled laboratory talent across the Anatomic and > Clinical Pathology specializations. > > ? No minimum commitment on number of weeks you need > laboratory staffing (Customized to your needs) > > ? For Sick Coverage there is 6 hour/day Minimum hours billed > > ? All inclusive bill rates (Travel/Pay Rate/Employment > Taxes, etc.) > > Candidates we have right now who can make an impact in your lab! > > Histotech Supervisor/ Manager (2 Available) > > * Certified Histotech (ASCP) with over 20 years of experience > * Seeking Temporary or Permanent work > * Location: Seeking Anywhere along the East coast from FL to ME, > West Coast > * Available: Now > > Histotech > > * ASCP Certified with 10 years of experience > * Primarily looking for Permanent but would also consider > Temp/Travel work > * Georgia > * Available: Starting in July 2023 > > Histotech (5 Available Candidates) > > * ASCP Certified and 5 years of experience > * Looking for Travel/Temp Position but open for Temp-Permanent > (North Carolina) > * Locations of interest: Anywhere in the US, Des Moines, IA, > South Charleston, SC, Birmingham, AL > * Available: Now > > Cytotechnologist-(1 Candidate Available) > > * Several years of experience with ASCP certification and Imaging > Trained > * Seeking primarily Temporary or Temporary to Permanent > * Locations of interest: Anywhere in US (Not State Licensed) > * Available: Now > > Medical Technologist-(7 Available Candidates) > > * Several years? experience as a Generalists > * Seeking Temporary/Travel > * Locations of Interest: Anywhere in US > * Availability: Now > > Medical Laboratory Technician-Generalist-(3 Available Candidates) > > * Has 2 years? experience and MLT Certification > * Seeking Permanent or Temporary Work > * Location seeking: Houston, TX area > * Available: Now > > Molecular Technologist ? (3 Candidates Available) > > * ASCP Molecular Specialists with 2+ years of experience > * Seeking Permanent, Temp to Permanent or Permanent > * Location: Anywhere in US > * Available: Now > > Phlebotomist- (2 Available Candidates) > > * Certified Phlebotomist (CPT) and ASCP Certified Phlebotomists > and 5-10 years of experience > * Seeking Temp/Travel Work Only > * Location Seeking: Anywhere in US > * Ability to Train other Phlebotomists: YES > * Available: Now > > Medical Assistant with Phlebotomy Experience- (2 Candidates > Available) > > * Has Medical Assistant Diploma with phlebotomy experience and > over 3 years? experience > * Seeking Temporary or Permanent work > * Location seeking: Houston, TX > * Available: April > > *If you do not see a professional on this list that fits with > what your talent needs are please inquire directly to discuss our > pipeline in your area and for your position.* > > Ask me about our Laboratory Training Program, Government > Services, Temporary or Permanent Staffing options! > > I'd love to connect. Here's my calendar link > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fcalendly.com%2Fmelissaowens > ) > to make finding time easy. > > Melissa Owens, CHP-ASA > > Allied Search Partners > > AN MRINETWORK MEMBER > > www.alliedsearchpartners.com > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=http%3A%2F%2Fwww.alliedsearchpartners.com > ) > > > Direct (Call) Line: 386 339 0839 > > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > Click here > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fapp.loxo.co%2Fagencies%2F13989%2Funsubscribe%2FadaTfk64aUjeYmZ8 > ) > to unsubscribe > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren at gmail.com Fri Jun 2 14:33:43 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Fri, 2 Jun 2023 14:33:43 -0500 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: Next thing you're going to be telling me it's not OK to embed with a 1 foot tall bunsen burner open flame and a Coke on the cold tray! On Fri, Jun 2, 2023 at 7:46?AM Piche, Jessica via Histonet < histonet at lists.utsouthwestern.edu> wrote: > It also doesn't set a good impression for everyone else in the lab since > they can't have a coffee at their stations. If it could be done, I wouldn't > want everyone else's coffees on my desk either, but I'd have to because > I'd want to make it fair to everyone. If only it was safe for all of us.... > > Have a great weekend everyone! > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory > Histology Team Leader > 203-573-7167 > ________________________________ > From: McLaughlin, Stacy L. via Histonet > > Sent: Friday, June 2, 2023 6:09 AM > To: Hannen, Valerie ; > Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Coffee at the desk > > [EXTERNAL MSG] > > It is not allowed. The area you want food/drink has to be completely > separate- floor to ceiling walls and a door. > > Stacy McLaughlin, HT(ASCP) QLScm > Histology Supervisor > Cooley Dickinson Hospital > 30 Locust Street > Northampton, MA 01060 > Office: (413)582-2019 > Lab: (413)582-2179 > smclaughlin7 at cooleydickinson.org > > > > > -----Original Message----- > From: Hannen, Valerie via Histonet > Sent: Thursday, June 1, 2023 3:40 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Coffee at the desk > > External Email - Use Caution > > Is there any regulation/ reason why a Histology supervisor whose desk is > in the department and the desk area is taped off and designated as a "clean > area ( meaning no chemicals or specimens cross the taped off area) can't > have a closed coffee cup at their desk?? We are having a debate in our Lab > and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=j200BRfpFcuOoSsxlA72ykoKjkqRHJDLCC7ISOWAuNE%3D&reserved=0 > < > http://secure-web.cisco.com/1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q/http%3A%2F%2Fwww.parrishmed.com > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailman%252Flistinfo%252Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=q9q1npoiwckRyrsVmqjSJu7zA2wsQGQSoNDGiBxYZtI%3D&reserved=0 > < > http://secure-web.cisco.com/1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > > > The information in this e-mail is intended only for the person to whom it > is addressed. If you believe this e-mail was sent to you in error and the > e-mail contains patient information, please contact the Mass General > Brigham Compliance HelpLine at > https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.massgeneralbrigham.org%2Fcomplianceline&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=FTM1qMH8yaSCp52d91TXceTwZhGHrd%2BFVUCLvaLccyc%3D&reserved=0 > < > https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.massgeneralbrigham.org%2Fcomplianceline&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=FTM1qMH8yaSCp52d91TXceTwZhGHrd%2BFVUCLvaLccyc%3D&reserved=0 > > . > Please note that this e-mail is not secure (encrypted). If you do not > wish to continue communication over unencrypted e-mail, please notify the > sender of this message immediately. Continuing to send or respond to > e-mail after receiving this message means you understand and accept this > risk and wish to continue to communicate over unencrypted e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0s4%2FqlgUO580fR5tpdGAJJDWE3%2FTSUw2q%2Fh2rfawKT4%3D&reserved=0 > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From akbitting at geisinger.edu Fri Jun 2 14:36:52 2023 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Fri, 2 Jun 2023 19:36:52 +0000 Subject: [Histonet] [External] Re: Coffee at the desk In-Reply-To: References: Message-ID: Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the VIP without fear of admonishment. -----Original Message----- From: Jay Lundgren via Histonet Sent: Friday, June 2, 2023 3:34 PM To: Piche, Jessica Cc: Histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Coffee at the desk External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Next thing you're going to be telling me it's not OK to embed with a 1 foot tall bunsen burner open flame and a Coke on the cold tray! On Fri, Jun 2, 2023 at 7:46?AM Piche, Jessica via Histonet < histonet at lists.utsouthwestern.edu> wrote: > It also doesn't set a good impression for everyone else in the lab > since they can't have a coffee at their stations. If it could be done, > I wouldn't want everyone else's coffees on my desk either, but I'd > have to because I'd want to make it fair to everyone. If only it was safe for all of us.... > > Have a great weekend everyone! > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory Histology Team Leader > 203-573-7167 > ________________________________ > From: McLaughlin, Stacy L. via Histonet > > > Sent: Friday, June 2, 2023 6:09 AM > To: Hannen, Valerie ; > Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Coffee at the desk > > [EXTERNAL MSG] > > It is not allowed. The area you want food/drink has to be completely > separate- floor to ceiling walls and a door. > > Stacy McLaughlin, HT(ASCP) QLScm > Histology Supervisor > Cooley Dickinson Hospital > 30 Locust Street > Northampton, MA 01060 > Office: (413)582-2019 > Lab: (413)582-2179 > smclaughlin7 at cooleydickinson.org > > > > > -----Original Message----- > From: Hannen, Valerie via Histonet > Sent: Thursday, June 1, 2023 3:40 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Coffee at the desk > > External Email - Use Caution > > Is there any regulation/ reason why a Histology supervisor whose desk > is in the department and the desk area is taped off and designated as > a "clean area ( meaning no chemicals or specimens cross the taped off > area) can't have a closed coffee cup at their desk?? We are having a > debate in our Lab and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish > Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > > http://secur/ > e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3g > Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKY > CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5X > UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZ > IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq8 > 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Cakb > itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c567c > 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFpbG > Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0% > 3D%7C3000%7C%7C%7C&sdata=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkkn0 > %3D&reserved=0 > < > http://secur/ > e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3g > Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKY > CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5X > UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZ > IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq8 > 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Cakb > itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c567c > 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFpbG > Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0% > 3D%7C3000%7C%7C%7C&sdata=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkkn0 > %3D&reserved=0 > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://secur/ > e-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQ > emY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnV > QpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubk > Xlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3 > cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclM > pgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailman% > 252Flistinfo%252Fhistonet&data=05%7C01%7Cakbitting%40geisinger.edu%7C4 > f92290a707e4df1d87108db63a05f02%7C37d46c567c664402a16055c2313b910d%7C0 > %7C0%7C638213312668777202%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAi > LCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=I > jVD7jHq5spkbK4qPhkeHkj%2BsPGgVYCeYatDv3x%2FOz4%3D&reserved=0 > < > http://secur/ > e-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQ > emY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnV > QpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubk > Xlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3 > cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclM > pgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailman% > 252Flistinfo%252Fhistonet&data=05%7C01%7Cakbitting%40geisinger.edu%7C4 > f92290a707e4df1d87108db63a05f02%7C37d46c567c664402a16055c2313b910d%7C0 > %7C0%7C638213312668777202%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAi > LCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=I > jVD7jHq5spkbK4qPhkeHkj%2BsPGgVYCeYatDv3x%2FOz4%3D&reserved=0 > > > > The information in this e-mail is intended only for the person to whom > it is addressed. 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From akemiat3377 at gmail.com Fri Jun 2 17:49:26 2023 From: akemiat3377 at gmail.com (Akemi Allison) Date: Fri, 2 Jun 2023 15:49:26 -0700 Subject: [Histonet] [External] Re: Coffee at the desk In-Reply-To: References: Message-ID: <9EB3CF33-4C1E-4E1A-B90E-E590EE73A472@gmail.com> 15:4-Observing Standard Precautions Occupational Safety and Health Administration (OSHA) Can a health care worker drink coffee in a laboratory where blood/body fluids/tissue tests are performed? Why or Why not? No, federal law prohibits eating and drinking in any area that can be potentially contaminated by blood or other body fluids. Regards, Akemi Allison-Tacha, BS, HT/HTL ASCP President/Consultant Phoenix Laboratory Consulting Sent from my iPhone On Jun 2, 2023, at 12:37 PM, Bitting, Angela K. via Histonet wrote: ?Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the VIP without fear of admonishment. -----Original Message----- From: Jay Lundgren via Histonet Sent: Friday, June 2, 2023 3:34 PM To: Piche, Jessica Cc: Histonet at lists.utsouthwestern.edu Subject: [External] Re: [Histonet] Coffee at the desk External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Next thing you're going to be telling me it's not OK to embed with a 1 foot tall bunsen burner open flame and a Coke on the cold tray! On Fri, Jun 2, 2023 at 7:46?AM Piche, Jessica via Histonet < histonet at lists.utsouthwestern.edu> wrote: It also doesn't set a good impression for everyone else in the lab since they can't have a coffee at their stations. If it could be done, I wouldn't want everyone else's coffees on my desk either, but I'd have to because I'd want to make it fair to everyone. If only it was safe for all of us.... Have a great weekend everyone! Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: McLaughlin, Stacy L. via Histonet ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coffee at the desk [EXTERNAL MSG] It is not allowed. The area you want food/drink has to be completely separate- floor to ceiling walls and a door. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughlin7 at cooleydickinson.org -----Original Message----- From: Hannen, Valerie via Histonet Sent: Thursday, June 1, 2023 3:40 PM To: Histonet at lists.utsouthwestern.edu Subject: [Histonet] Coffee at the desk External Email - Use Caution Is there any regulation/ reason why a Histology supervisor whose desk is in the department and the desk area is taped off and designated as a "clean area ( meaning no chemicals or specimens cross the taped off area) can't have a closed coffee cup at their desk?? We are having a debate in our Lab and I wanted to get a consensus. Thanks in Advance, Valerie Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Avenue Titusville, Florida 32796 P: 321-268-6333 Ext. 7506 F: 321-268-6149 valerie.hannen at parrishmed.com http://secur/ e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr 3g Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__w KY CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_ 5X UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9 IZ IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuY q8 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn 0% 3D%7C3000%7C%7C%7C&sdata=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkk n0 %3D&reserved=0 < http://secur/ e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr 3g Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__w KY CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_ 5X UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9 IZ IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuY q8 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn 0% 3D%7C3000%7C%7C%7C&sdata=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkk n0 %3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secur/ e-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431 oQ emY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7Gh nV QpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEu bk Xlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJH V3 cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAc lM pgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailma n% 252Flistinfo%252Fhistonet&data=05%7C01%7Cakbitting%40geisinger.edu%7 C4 f92290a707e4df1d87108db63a05f02%7C37d46c567c664402a16055c2313b910d%7 C0 %7C0%7C638213312668777202%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMD Ai LCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata =I jVD7jHq5spkbK4qPhkeHkj%2BsPGgVYCeYatDv3x%2FOz4%3D&reserved=0 < http://secur/ e-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431 oQ emY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7Gh nV QpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEu bk Xlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJH V3 cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAc lM pgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailma n% 252Flistinfo%252Fhistonet&data=05%7C01%7Cakbitting%40geisinger.edu%7 C4 f92290a707e4df1d87108db63a05f02%7C37d46c567c664402a16055c2313b910d%7 C0 %7C0%7C638213312668777202%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMD Ai LCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata =I jVD7jHq5spkbK4qPhkeHkj%2BsPGgVYCeYatDv3x%2FOz4%3D&reserved=0 The information in this e-mail is intended only for the person to whom it is addressed. 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Continuing to send or respond to e-mail after receiving this message means you understand and accept this risk and wish to continue to communicate over unencrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists/ .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn 0% 3D%7C3000%7C%7C%7C&sdata=KbpFIM8cidcpS%2FO520oZ%2BlpH3TcWJHPAKoxOtPY lI h0%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists/ .utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Ca kb itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c56 7c 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFp bG Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn 0% 3D%7C3000%7C%7C%7C&sdata=KbpFIM8cidcpS%2FO520oZ%2BlpH3TcWJHPAKoxOtPY lI h0%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan at uwo.ca Fri Jun 2 21:53:29 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Sat, 3 Jun 2023 02:53:29 +0000 Subject: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need In-Reply-To: References: <20230602153820.b4a91a6022ad1016@alliedsearchpartners.com> Message-ID: Hear, Hear! Let's see less advertising on Histonet. John Kiernan (London, Canada). = = = ________________________________ From: Jay Lundgren via Histonet Sent: June 2, 2023 1:15 PM To: Melissa Owens Cc: Tom Walls Subject: Re: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need As far as I know, Histonet doesn't really allow advertising for individuals or agencies *looking* for jobs. There are a very few, selective, agencies that are allowed to post on Histonet tastefully and infrequently, but they are buying, not selling. Or histotechs who let others know about open positions in their organization. That's cool. I'm not a moderator, but this is supposed to be a collegial forum for practical histopathology advice. There are plenty of job search sites out there already. But if Histonet does start allowing this, then ***%%%$$$HEY SCRIPPS INSTITUTE HIRE ME!!!!$$$%%%%*** ***%%%$$$LONG OR SHORT CONTRACT!!!CHEAP$$$%%%*** **%%%$$$BIG TIME IMUNNOHISTOCHEMISTRY!!!!$$$%%%*** ***%%%$$$USAF HTL (ASCP) M.S. 30+ YRS!!!!!!!!!!!$$$%%%*** Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 2, 2023 at 10:50?AM 'Melissa Owens' via Histonet < histonet at lists.utsouthwestern.edu> wrote: > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > When workloads spike, unexpected projects hit, employees are out > for PTO, Sick or Parental Leave, we got your Lab Covered! Our > Staffing Services for the lab can get the work done quickly. We > have skilled laboratory talent at the ready and range of > temporary staffing options to meet your unique needs. > > ? Short Term/Leave Coverage > > ? Last Minute Sick Coverage > > ? Long Term Leave Coverage > > ? Long Term Laboratory Training Program > > ? Contract to Hire (Asses a candidate on the job before > committing to hiring them for a permanent role) > > We place skilled laboratory talent across the Anatomic and > Clinical Pathology specializations. > > ? No minimum commitment on number of weeks you need > laboratory staffing (Customized to your needs) > > ? For Sick Coverage there is 6 hour/day Minimum hours billed > > ? All inclusive bill rates (Travel/Pay Rate/Employment > Taxes, etc.) > > Candidates we have right now who can make an impact in your lab! > > Histotech Supervisor/ Manager (2 Available) > > * Certified Histotech (ASCP) with over 20 years of experience > * Seeking Temporary or Permanent work > * Location: Seeking Anywhere along the East coast from FL to ME, > West Coast > * Available: Now > > Histotech > > * ASCP Certified with 10 years of experience > * Primarily looking for Permanent but would also consider > Temp/Travel work > * Georgia > * Available: Starting in July 2023 > > Histotech (5 Available Candidates) > > * ASCP Certified and 5 years of experience > * Looking for Travel/Temp Position but open for Temp-Permanent > (North Carolina) > * Locations of interest: Anywhere in the US, Des Moines, IA, > South Charleston, SC, Birmingham, AL > * Available: Now > > Cytotechnologist-(1 Candidate Available) > > * Several years of experience with ASCP certification and Imaging > Trained > * Seeking primarily Temporary or Temporary to Permanent > * Locations of interest: Anywhere in US (Not State Licensed) > * Available: Now > > Medical Technologist-(7 Available Candidates) > > * Several years? experience as a Generalists > * Seeking Temporary/Travel > * Locations of Interest: Anywhere in US > * Availability: Now > > Medical Laboratory Technician-Generalist-(3 Available Candidates) > > * Has 2 years? experience and MLT Certification > * Seeking Permanent or Temporary Work > * Location seeking: Houston, TX area > * Available: Now > > Molecular Technologist ? (3 Candidates Available) > > * ASCP Molecular Specialists with 2+ years of experience > * Seeking Permanent, Temp to Permanent or Permanent > * Location: Anywhere in US > * Available: Now > > Phlebotomist- (2 Available Candidates) > > * Certified Phlebotomist (CPT) and ASCP Certified Phlebotomists > and 5-10 years of experience > * Seeking Temp/Travel Work Only > * Location Seeking: Anywhere in US > * Ability to Train other Phlebotomists: YES > * Available: Now > > Medical Assistant with Phlebotomy Experience- (2 Candidates > Available) > > * Has Medical Assistant Diploma with phlebotomy experience and > over 3 years? experience > * Seeking Temporary or Permanent work > * Location seeking: Houston, TX > * Available: April > > *If you do not see a professional on this list that fits with > what your talent needs are please inquire directly to discuss our > pipeline in your area and for your position.* > > Ask me about our Laboratory Training Program, Government > Services, Temporary or Permanent Staffing options! > > I'd love to connect. Here's my calendar link > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fcalendly.com%2Fmelissaowens > ) > to make finding time easy. > > Melissa Owens, CHP-ASA > > Allied Search Partners > > AN MRINETWORK MEMBER > > www.alliedsearchpartners.com > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=http%3A%2F%2Fwww.alliedsearchpartners.com > ) > > > Direct (Call) Line: 386 339 0839 > > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > Click here > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fapp.loxo.co%2Fagencies%2F13989%2Funsubscribe%2FadaTfk64aUjeYmZ8 > ) > to unsubscribe > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From afhenwood at outlook.com Fri Jun 2 23:17:56 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Sat, 3 Jun 2023 04:17:56 +0000 Subject: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need In-Reply-To: References: <20230602153820.b4a91a6022ad1016@alliedsearchpartners.com> Message-ID: I agree Regards, Tony Henwood Sydney, Australia From: John Kiernan via Histonet Sent: Saturday, 3 June 2023 11:09 AM To: Melissa Owens; Jay Lundgren Cc: Tom Walls Subject: Re: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need Hear, Hear! Let's see less advertising on Histonet. John Kiernan (London, Canada). = = = ________________________________ From: Jay Lundgren via Histonet Sent: June 2, 2023 1:15 PM To: Melissa Owens Cc: Tom Walls Subject: Re: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need As far as I know, Histonet doesn't really allow advertising for individuals or agencies *looking* for jobs. There are a very few, selective, agencies that are allowed to post on Histonet tastefully and infrequently, but they are buying, not selling. Or histotechs who let others know about open positions in their organization. That's cool. I'm not a moderator, but this is supposed to be a collegial forum for practical histopathology advice. There are plenty of job search sites out there already. But if Histonet does start allowing this, then ***%%%$$$HEY SCRIPPS INSTITUTE HIRE ME!!!!$$$%%%%*** ***%%%$$$LONG OR SHORT CONTRACT!!!CHEAP$$$%%%*** **%%%$$$BIG TIME IMUNNOHISTOCHEMISTRY!!!!$$$%%%*** ***%%%$$$USAF HTL (ASCP) M.S. 30+ YRS!!!!!!!!!!!$$$%%%*** Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Jun 2, 2023 at 10:50?AM 'Melissa Owens' via Histonet < histonet at lists.utsouthwestern.edu> wrote: > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > When workloads spike, unexpected projects hit, employees are out > for PTO, Sick or Parental Leave, we got your Lab Covered! Our > Staffing Services for the lab can get the work done quickly. We > have skilled laboratory talent at the ready and range of > temporary staffing options to meet your unique needs. > > ? Short Term/Leave Coverage > > ? Last Minute Sick Coverage > > ? Long Term Leave Coverage > > ? Long Term Laboratory Training Program > > ? Contract to Hire (Asses a candidate on the job before > committing to hiring them for a permanent role) > > We place skilled laboratory talent across the Anatomic and > Clinical Pathology specializations. > > ? No minimum commitment on number of weeks you need > laboratory staffing (Customized to your needs) > > ? For Sick Coverage there is 6 hour/day Minimum hours billed > > ? All inclusive bill rates (Travel/Pay Rate/Employment > Taxes, etc.) > > Candidates we have right now who can make an impact in your lab! > > Histotech Supervisor/ Manager (2 Available) > > * Certified Histotech (ASCP) with over 20 years of experience > * Seeking Temporary or Permanent work > * Location: Seeking Anywhere along the East coast from FL to ME, > West Coast > * Available: Now > > Histotech > > * ASCP Certified with 10 years of experience > * Primarily looking for Permanent but would also consider > Temp/Travel work > * Georgia > * Available: Starting in July 2023 > > Histotech (5 Available Candidates) > > * ASCP Certified and 5 years of experience > * Looking for Travel/Temp Position but open for Temp-Permanent > (North Carolina) > * Locations of interest: Anywhere in the US, Des Moines, IA, > South Charleston, SC, Birmingham, AL > * Available: Now > > Cytotechnologist-(1 Candidate Available) > > * Several years of experience with ASCP certification and Imaging > Trained > * Seeking primarily Temporary or Temporary to Permanent > * Locations of interest: Anywhere in US (Not State Licensed) > * Available: Now > > Medical Technologist-(7 Available Candidates) > > * Several years? experience as a Generalists > * Seeking Temporary/Travel > * Locations of Interest: Anywhere in US > * Availability: Now > > Medical Laboratory Technician-Generalist-(3 Available Candidates) > > * Has 2 years? experience and MLT Certification > * Seeking Permanent or Temporary Work > * Location seeking: Houston, TX area > * Available: Now > > Molecular Technologist ? (3 Candidates Available) > > * ASCP Molecular Specialists with 2+ years of experience > * Seeking Permanent, Temp to Permanent or Permanent > * Location: Anywhere in US > * Available: Now > > Phlebotomist- (2 Available Candidates) > > * Certified Phlebotomist (CPT) and ASCP Certified Phlebotomists > and 5-10 years of experience > * Seeking Temp/Travel Work Only > * Location Seeking: Anywhere in US > * Ability to Train other Phlebotomists: YES > * Available: Now > > Medical Assistant with Phlebotomy Experience- (2 Candidates > Available) > > * Has Medical Assistant Diploma with phlebotomy experience and > over 3 years? experience > * Seeking Temporary or Permanent work > * Location seeking: Houston, TX > * Available: April > > *If you do not see a professional on this list that fits with > what your talent needs are please inquire directly to discuss our > pipeline in your area and for your position.* > > Ask me about our Laboratory Training Program, Government > Services, Temporary or Permanent Staffing options! > > I'd love to connect. Here's my calendar link > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fcalendly.com%2Fmelissaowens > ) > to make finding time easy. > > Melissa Owens, CHP-ASA > > Allied Search Partners > > AN MRINETWORK MEMBER > > www.alliedsearchpartners.com > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=http%3A%2F%2Fwww.alliedsearchpartners.com > ) > > > Direct (Call) Line: 386 339 0839 > > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > Click here > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fapp.loxo.co%2Fagencies%2F13989%2Funsubscribe%2FadaTfk64aUjeYmZ8 > ) > to unsubscribe > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sat Jun 3 07:25:29 2023 From: rsrichmond at gmail.com (Bob Richmond) Date: Sat, 3 Jun 2023 08:25:29 -0400 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: This 84 year old pathologist recalls the histopathology laboratory at Johns Hopkins Hospital around 1970, when I was a pathology resident there. Histotechs, often laboratory clerks, sat in front of rows of 400 mL Stender dishes, smoking cigarettes while they hand-stained slides, often carrying out the entire procedure from xylene and descending alcohols, up to final coverslipping. It wasn't the xylene that worried me, so much as the dish full of a 20% solution of picric acid in acetone, that removed most of the copious formalin pigment (since buffering the formalin wasn't permitted). I spoke to the chief histotechnologist about the issue. He responded by stubbing out a lighted cigarette into one of the Stender dishes of xylene. (I'm told you can also do that with gasoline, but not with acetone.) He was never incinerated, but he died of smoking-related disease soon after his retirement. Bob Richmond Maryville TN From jkiernan at uwo.ca Sat Jun 3 23:57:46 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Sun, 4 Jun 2023 04:57:46 +0000 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: Bob, Why weren't they routinely buffering (or at least neutralizing) the formalin fixatives at Johns Hopkins as recently as 1970? It had all been in the scholarly books (by Pearse, Lillie, etc) for >10 years, and was also in Lee Luna's 1968 Manual of Histologic Staining Methods, published by the USA's Armed Forces Institute of Pathology. I was brung up on cold neutral buffered formalin for enzyme activity histochemistry when I was a medical student doing an interpolated research year in the early 1960s. Cheers, John John Kiernan (London, Canada) = = = ________________________________ From: Bob Richmond via Histonet Sent: June 3, 2023 8:25 AM To: Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Coffee at the desk This 84 year old pathologist recalls the histopathology laboratory at Johns Hopkins Hospital around 1970, when I was a pathology resident there. Histotechs, often laboratory clerks, sat in front of rows of 400 mL Stender dishes, smoking cigarettes while they hand-stained slides, often carrying out the entire procedure from xylene and descending alcohols, up to final coverslipping. It wasn't the xylene that worried me, so much as the dish full of a 20% solution of picric acid in acetone, that removed most of the copious formalin pigment (since buffering the formalin wasn't permitted). I spoke to the chief histotechnologist about the issue. He responded by stubbing out a lighted cigarette into one of the Stender dishes of xylene. (I'm told you can also do that with gasoline, but not with acetone.) He was never incinerated, but he died of smoking-related disease soon after his retirement. Bob Richmond Maryville TN _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond at gmail.com Sun Jun 4 13:19:22 2023 From: rsrichmond at gmail.com (Bob Richmond) Date: Sun, 4 Jun 2023 14:19:22 -0400 Subject: [Histonet] Coffee at the desk In-Reply-To: References: Message-ID: John Kiernan asks: > > >>Bob, Why weren't they routinely buffering (or at least neutralizing) the > formalin fixatives at Johns Hopkins as recently as 1970? - It had all been > in the scholarly books (by Pearse, Lillie, etc) for >10 years, and was also > in Lee Luna's 1968 Manual of Histologic Staining Methods, published by the > USA's Armed Forces Institute of Pathology.<< > Quite so, but the tyrannical (and seriously dyslexic) chief histotechnologist did not permit any sort of buffering, not even the then-venerable (and quite ineffective) chips of marble (calcium carbonate) in the bottom of the bottle. Ralph Lillie's book (which I still have, along with the AFIP manual) failed to specify sodium phosphate dibasic anhydrous (Na2HPO4) in the formula, though Lee Luna did. Unfortunately, the heptahydrate salt (which is nearly half water) was also available, and since it was the cheapest product in the catalog, people usually ordered it, and I suppose that's what the guy at JHH had done. - If you made up Lillie's formula using the heptahydrate salt, without doubling the quantity you added, you got a pH of 6.1. In my travels I saw several times that a lab would make up the pH 6.1 buffer, then laboriously titrate in NaOH solution to bring the pH up to 7, using a pH meter. A time or two I recalculated the formula for the heptahydrate for them (in other words, multiplying by 1.9) and fixed the problem. He also didn't permit procedure manuals, requiring techs to memorize the special stains, the result being that most of them scribbled out procedures and hid them in their desk drawers. When I was near the end of my residency and wanted to take some of the methods with me, I'd laboriously assemble the procedure by borrowing those reference cards, which usually contained a lot of incorrect information. (One result of that was that I was the only person who preserved the Johns Hopkins eosin Y - phloxine B - Biebrich scarlet formula for the fabled JHH eosin stain, whose intensity very nearly seared the microscopist's eyeballs.) The senior pathologists tolerated all of this because they didn't know enough about what went on in the laboratory to take any corrective action. Pathologists trained then, and I guess now, thought that slides just sorta fell out of the sky. It was a valuable learning experience for me. I learned the importance of the pathologist knowing how to do almost everything in the histology lab. I think this experience ought to be part of every anatomic pathologist's training. Bob Richmond Maryville TN From relia1 at earthlink.net Tue Jun 6 09:43:22 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 6 Jun 2023 10:43:22 -0400 Subject: [Histonet] RELIA HOT Job Alert: In House Tech Support Full time permanRELIA HOT Job Alert!ent! Message-ID: <013301d99885$3e621d30$bb265790$@earthlink.net> Hi Histonetters, I noticed some traffic last week about who can post what and I just wanted to say Thank you for allowing me to post opportunities on this forum. Can you believe it has been over 18 years since my first post? Wow time sure flies when you're having fun. I wonder if anyone remembers the happy hour we had for the histonet at the NSH meeting in Fort Lauderdale in 2005! Also there is this: RELIA HOT Job Alert! Histology Tech Support position! Permanent full time position IN HOUSE/On-site in Wisconsin. Excellent compensation, benefits and opportunity for growth!! Msg me. email me at relia1 at earthlink.net or text/call me at 407-353-5070 for info! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From bianca at alliedsearchpartners.com Tue Jun 6 22:49:14 2023 From: bianca at alliedsearchpartners.com (Bianca Shalagin) Date: Wed, 7 Jun 2023 03:49:14 +0000 Subject: [Histonet] Histotech Opportunity Alert Huntsville, AL Message-ID: Allied Search Partners Histotech - Huntsville, AL Position Title: Histotech Shifts Available: 4 am to 12:30 pm Location: Huntsville, AL Pay: Up to $27-$35/hour Job Responsibilities The position is responsible for processing, embedding, cutting, and staining of tissue slides, accurate quality control, special projects, equipment maintenance, and all other duties assigned by the Team Lead or Laboratory Manager. Additional responsibilities of Histotech include. The jobs listed above, may or may not include all or some of these duties. * Performs both routine and complex special procedures with an understanding of tissue structures, techniques, principles, theory, and instrumentation. * May gross section and dictate descriptions of simple specimens, process, embed, cut and stain all types of tissue specimens received in the Histology Department. * Coverslip-stained sections, verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. * Assures specimen labeling requirements are met and necessary clinical information is available as needed. * Assists laboratory assistant or performs accessioning of patient specimens into the laboratory computer system when needed. * Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. * Selects and maintains appropriate tissue blocks to serve as controls for Special Stains. * Maintains all documentation that ensures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains and IHC. * Able to process and decal bone specimens. * Able to prioritize the processing of specimens. * Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. * Monitor quality control results and take immediate and proper action when controls are unacceptable. * Follow defined procedures with only Lab Manager or pathologist-approved modifications or deviations. * Complete all instrument function verification, maintenance, and documents according to the procedure in the Histology area. Ensure that equipment defects and malfunctions are reported and repaired. * Maintains a neat, clean, and orderly work area. At the end of the day's work; is responsible for the cleaning of his/her microtome. * Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. * May Cuts slides for IHC testing or other send-outs in conformance with procedures and submits them for analysis. * Must be able to express himself/herself effectively both in written and verbal communications. * Assists supervisory staff in monitoring workflow and ensuring that work/ staining priorities are met for the department. * Will be asked to put new tests/ stains online. * Participates in weekend and holiday schedules as staffing requirements dictate. * Remains flexible and works a share of overtime or different shifts if necessary during staffing shortages or emergencies. * Participates in continuing education classes and courses. Strongly encouraged to keep updated on recent advances in the field of histology and to take at least 10 credit hours of continuing education a year. * Highly encouraged to maintain membership in a professional Histology organization. * Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. Successful candidates for the Histotech role include: HT, HTL(ASCP) registry or eligible (not required) ASCP website: https://www.ascp.org/content/about-ascp/contact-ascp# NY license application website: https://www.op.nysed.gov/professions/clinical-laboratory-technologists/license-options FL license application website: https://floridasclinicallabs.gov/licensing/ CA license application website: https://www.cdph.ca.gov/Programs/OSPHLD/LFS/pages/clinicallaboratorypersonnel.aspx Making a big move? Here is a price to living page: https://www.bestplaces.net/ Histotech (Healthcare Sciences) To apply: Please send a resume to bianca at alliedsearchpartners.com Microsoft Word is preferred/ PDF is acceptable. To Schedule Meeting: https://calendly.com/bianca09/30min From flourish.jf at gmail.com Thu Jun 8 10:41:43 2023 From: flourish.jf at gmail.com (Janice Fuller) Date: Thu, 8 Jun 2023 08:41:43 -0700 Subject: [Histonet] Histonet Digest, Vol 235, Issue 1 In-Reply-To: References: Message-ID: Is there fresh tissue in that part of the lab? Or anything else that would cause it to be considered not clean? On Fri, Jun 2, 2023 at 10:00?AM wrote: > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Coffee at the desk (Hannen, Valerie) > 2. Re: Coffee at the desk (Thomas Podawiltz) > 3. Re: Coffee at the desk (McLaughlin, Stacy L.) > 4. Re: Coffee at the desk (Piche, Jessica) > 5. Get the Short Term or Long Term Lab Staffing Coverage You > Need ('Melissa Owens') > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 1 Jun 2023 19:40:24 +0000 > From: "Hannen, Valerie" > To: "Histonet at lists.utsouthwestern.edu" > > Subject: [Histonet] Coffee at the desk > Message-ID: > < > DM6PR15MB3210BC2A1B2252BE21FD076AF6499 at DM6PR15MB3210.namprd15.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > Is there any regulation/ reason why a Histology supervisor whose desk is > in the department and the desk area is taped off and designated as a "clean > area ( meaning no chemicals or specimens cross the taped off area) can't > have a closed coffee cup at their desk?? We are having a debate in our Lab > and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > > > ------------------------------ > > Message: 2 > Date: Thu, 1 Jun 2023 20:00:30 +0000 (UTC) > From: Thomas Podawiltz > To: "Histonet at lists.utsouthwestern.edu" > , "Hannen, Valerie" > > Subject: Re: [Histonet] Coffee at the desk > Message-ID: <162780967.2965690.1685649630442 at mail.yahoo.com> > Content-Type: text/plain; charset=UTF-8 > > OSHA: bloodborne pathogen standard.A clean area is an area that is closed > off from the working environment with walls and door. Tape is not a > appropriate alternative, maybe on WKRP in Cincinnati, but not in a lab.? > On Thursday, June 1, 2023 at 03:40:41 PM EDT, Hannen, Valerie via > Histonet wrote: > > Is there any regulation/ reason why a Histology supervisor whose desk is > in the department and the desk area is taped off and designated as a "clean > area (? meaning no chemicals or specimens cross the taped off area) can't > have a closed coffee cup at their desk??? We are having a debate in our Lab > and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333? Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > www.parrishmed.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > | | Virus-free.www.avast.com | > > > > ------------------------------ > > Message: 3 > Date: Fri, 2 Jun 2023 10:09:41 +0000 > From: "McLaughlin, Stacy L." > To: "Hannen, Valerie" , > "Histonet at lists.utsouthwestern.edu" > > Subject: Re: [Histonet] Coffee at the desk > Message-ID: > < > CO6PR04MB8313971F3F68E4F2822A97629B4EA at CO6PR04MB8313.namprd04.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > It is not allowed. The area you want food/drink has to be completely > separate- floor to ceiling walls and a door. > > Stacy McLaughlin, HT(ASCP) QLScm > Histology Supervisor > Cooley Dickinson Hospital > 30 Locust Street > Northampton, MA 01060 > Office: (413)582-2019 > Lab: (413)582-2179 > smclaughlin7 at cooleydickinson.org > > > > > -----Original Message----- > From: Hannen, Valerie via Histonet > Sent: Thursday, June 1, 2023 3:40 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Coffee at the desk > > External Email - Use Caution > > Is there any regulation/ reason why a Histology supervisor whose desk is > in the department and the desk area is taped off and designated as a "clean > area ( meaning no chemicals or specimens cross the taped off area) can't > have a closed coffee cup at their desk?? We are having a debate in our Lab > and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > > http://secure-web.cisco.com/1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q/http%3A%2F%2Fwww.parrishmed.com > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > http://secure-web.cisco.com/1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > The information in this e-mail is intended only for the person to whom it > is addressed. If you believe this e-mail was sent to you in error and the > e-mail contains patient information, please contact the Mass General > Brigham Compliance HelpLine at > https://www.massgeneralbrigham.org/complianceline < > https://www.massgeneralbrigham.org/complianceline> . > Please note that this e-mail is not secure (encrypted). If you do not > wish to continue communication over unencrypted e-mail, please notify the > sender of this message immediately. Continuing to send or respond to > e-mail after receiving this message means you understand and accept this > risk and wish to continue to communicate over unencrypted e-mail. > > > > > ------------------------------ > > Message: 4 > Date: Fri, 2 Jun 2023 12:36:52 +0000 > From: "Piche, Jessica" > To: "Hannen, Valerie" , > "Histonet at lists.utsouthwestern.edu" > , "McLaughlin, Stacy L." > > Subject: Re: [Histonet] Coffee at the desk > Message-ID: > < > BL0PR18MB2083D00128B9AF0D122D85FA8F4EA at BL0PR18MB2083.namprd18.prod.outlook.com > > > > Content-Type: text/plain; charset="us-ascii" > > It also doesn't set a good impression for everyone else in the lab since > they can't have a coffee at their stations. If it could be done, I wouldn't > want everyone else's coffees on my desk either, but I'd have to because > I'd want to make it fair to everyone. If only it was safe for all of us.... > > Have a great weekend everyone! > > Jessica > > Jessica Piche, HT(ASCP) > Waterbury Hospital Histology Laboratory > Histology Team Leader > 203-573-7167 > ________________________________ > From: McLaughlin, Stacy L. via Histonet > > Sent: Friday, June 2, 2023 6:09 AM > To: Hannen, Valerie ; > Histonet at lists.utsouthwestern.edu > Subject: Re: [Histonet] Coffee at the desk > > [EXTERNAL MSG] > > It is not allowed. The area you want food/drink has to be completely > separate- floor to ceiling walls and a door. > > Stacy McLaughlin, HT(ASCP) QLScm > Histology Supervisor > Cooley Dickinson Hospital > 30 Locust Street > Northampton, MA 01060 > Office: (413)582-2019 > Lab: (413)582-2179 > smclaughlin7 at cooleydickinson.org > > > > > -----Original Message----- > From: Hannen, Valerie via Histonet > Sent: Thursday, June 1, 2023 3:40 PM > To: Histonet at lists.utsouthwestern.edu > Subject: [Histonet] Coffee at the desk > > External Email - Use Caution > > Is there any regulation/ reason why a Histology supervisor whose desk is > in the department and the desk area is taped off and designated as a "clean > area ( meaning no chemicals or specimens cross the taped off area) can't > have a closed coffee cup at their desk?? We are having a debate in our Lab > and I wanted to get a consensus. > > Thanks in Advance, > > Valerie > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.hannen at parrishmed.com > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=j200BRfpFcuOoSsxlA72ykoKjkqRHJDLCC7ISOWAuNE%3D&reserved=0 > < > http://secure-web.cisco.com/1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3gTc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKYCLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5XUvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZIGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq86VfPp5oSQ8Q/http%3A%2F%2Fwww.parrishmed.com > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fsecure-web.cisco.com%2F1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg%2Fhttp%253A%252F%252Flists.utsouthwestern.edu%252Fmailman%252Flistinfo%252Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=q9q1npoiwckRyrsVmqjSJu7zA2wsQGQSoNDGiBxYZtI%3D&reserved=0 > < > http://secure-web.cisco.com/1f0gJaUlhvSZVf6wFKqwqiGqwPIeXbShsG3gx6JxyhlJfdb431oQemY-YoKwSi6bFWfJYQe2KigY4li7ljT9EWJjiQFlcuEBe-rD5Ad8A-qKNSXiEOSJF7GhnVQpCyI1OEllux9uMQ7JELDuoWq_CV9VJILMtYsIYxHWV8nJZ0O4x_qCwYQB3tmvs8bhEubkXlr_hL6Apd8hHYr3W0nI73dV0gWlT9wBokgsnbP7r00Pajz371Q2jg_c_INQgcazrFJHV3cYBLJtCEs7pQexlUtOAPCr_5dhmlHSVdORIgSKay3dv-Xfh-QyUS8_N2ujrnRFdPgDAclMpgkPs-ZJ7Wg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet > > > > The information in this e-mail is intended only for the person to whom it > is addressed. 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Continuing to send or respond to > e-mail after receiving this message means you understand and accept this > risk and wish to continue to communicate over unencrypted e-mail. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > > https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjessica.piche%40wtbyhosp.org%7Ca4730e2150334508bd1208db63518b30%7Cd4e8b650c4e4424481b4a11e2edb35a9%7C0%7C0%7C638212974104850946%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0s4%2FqlgUO580fR5tpdGAJJDWE3%2FTSUw2q%2Fh2rfawKT4%3D&reserved=0 > > > > ------------------------------ > > Message: 5 > Date: Fri, 02 Jun 2023 15:38:20 +0000 > From: 'Melissa Owens' > To: 'Tom Walls' > Subject: [Histonet] Get the Short Term or Long Term Lab Staffing > Coverage You Need > Message-ID: <20230602153820.b4a91a6022ad1016 at alliedsearchpartners.com> > Content-Type: text/plain; charset="utf-8" > > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > When workloads spike, unexpected projects hit, employees are out > for PTO, Sick or Parental Leave, we got your Lab Covered! Our > Staffing Services for the lab can get the work done quickly. We > have skilled laboratory talent at the ready and range of > temporary staffing options to meet your unique needs. > > ? Short Term/Leave Coverage > > ? Last Minute Sick Coverage > > ? Long Term Leave Coverage > > ? Long Term Laboratory Training Program > > ? Contract to Hire (Asses a candidate on the job before > committing to hiring them for a permanent role) > > We place skilled laboratory talent across the Anatomic and > Clinical Pathology specializations. > > ? No minimum commitment on number of weeks you need > laboratory staffing (Customized to your needs) > > ? For Sick Coverage there is 6 hour/day Minimum hours billed > > ? All inclusive bill rates (Travel/Pay Rate/Employment > Taxes, etc.) > > Candidates we have right now who can make an impact in your lab! > > Histotech Supervisor/ Manager (2 Available) > > * Certified Histotech (ASCP) with over 20 years of experience > * Seeking Temporary or Permanent work > * Location: Seeking Anywhere along the East coast from FL to ME, > West Coast > * Available: Now > > Histotech > > * ASCP Certified with 10 years of experience > * Primarily looking for Permanent but would also consider > Temp/Travel work > * Georgia > * Available: Starting in July 2023 > > Histotech (5 Available Candidates) > > * ASCP Certified and 5 years of experience > * Looking for Travel/Temp Position but open for Temp-Permanent > (North Carolina) > * Locations of interest: Anywhere in the US, Des Moines, IA, > South Charleston, SC, Birmingham, AL > * Available: Now > > Cytotechnologist-(1 Candidate Available) > > * Several years of experience with ASCP certification and Imaging > Trained > * Seeking primarily Temporary or Temporary to Permanent > * Locations of interest: Anywhere in US (Not State Licensed) > * Available: Now > > Medical Technologist-(7 Available Candidates) > > * Several years? experience as a Generalists > * Seeking Temporary/Travel > * Locations of Interest: Anywhere in US > * Availability: Now > > Medical Laboratory Technician-Generalist-(3 Available Candidates) > > * Has 2 years? experience and MLT Certification > * Seeking Permanent or Temporary Work > * Location seeking: Houston, TX area > * Available: Now > > Molecular Technologist ? (3 Candidates Available) > > * ASCP Molecular Specialists with 2+ years of experience > * Seeking Permanent, Temp to Permanent or Permanent > * Location: Anywhere in US > * Available: Now > > Phlebotomist- (2 Available Candidates) > > * Certified Phlebotomist (CPT) and ASCP Certified Phlebotomists > and 5-10 years of experience > * Seeking Temp/Travel Work Only > * Location Seeking: Anywhere in US > * Ability to Train other Phlebotomists: YES > * Available: Now > > Medical Assistant with Phlebotomy Experience- (2 Candidates > Available) > > * Has Medical Assistant Diploma with phlebotomy experience and > over 3 years? experience > * Seeking Temporary or Permanent work > * Location seeking: Houston, TX > * Available: April > > *If you do not see a professional on this list that fits with > what your talent needs are please inquire directly to discuss our > pipeline in your area and for your position.* > > Ask me about our Laboratory Training Program, Government > Services, Temporary or Permanent Staffing options! > > I'd love to connect. Here's my calendar link > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fcalendly.com%2Fmelissaowens > ) > to make finding time easy. > > Melissa Owens, CHP-ASA > > Allied Search Partners > > AN MRINETWORK MEMBER > > www.alliedsearchpartners.com > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=http%3A%2F%2Fwww.alliedsearchpartners.com > ) > > > Direct (Call) Line: 386 339 0839 > > li {display:list-item;text-indent: -1em;} > ul, ol{margin-left: 40px !important; padding-left: 0px > !important;} > > Click here > ( > https://app.loxo.co/agencies/13989/email_tracking/click?id=104910928&url=https%3A%2F%2Fapp.loxo.co%2Fagencies%2F13989%2Funsubscribe%2FadaTfk64aUjeYmZ8 > ) > to unsubscribe > > ------------------------------ > > Subject: Digest Footer > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > End of Histonet Digest, Vol 235, Issue 1 > **************************************** > -- *Janice Fuller* From kendra.beechie at commonspirit.org Mon Jun 12 14:22:08 2023 From: kendra.beechie at commonspirit.org (Kendra Beechie ND-Bismarck) Date: Mon, 12 Jun 2023 14:22:08 -0500 Subject: [Histonet] Peroxidase stain on peripheral smears Message-ID: Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From kdean70 at hotmail.com Mon Jun 12 15:34:52 2023 From: kdean70 at hotmail.com (Ken M) Date: Mon, 12 Jun 2023 20:34:52 +0000 Subject: [Histonet] MOHS staining Message-ID: For those doing MOHS staining either manually or automatically. How many staining cups do you really use? Are six enough if there is a running water cup nearby or do you need more? Ken From jkiernan at uwo.ca Mon Jun 12 23:46:26 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Tue, 13 Jun 2023 04:46:26 +0000 Subject: [Histonet] Peroxidase stain on peripheral smears In-Reply-To: References: Message-ID: What is the Kaplow method? I can't find it in textbooks. A quick Google search brings up only junk papers indicating that a Kaplov method may use carcinogenic benzidine (with wrong spelling) as the chromogen. There are simple, safe and inexpensive methods for histochemical localization of sites of peroxidase activity.in tissue sections or smears of cells. Buy a textbook for about $50, read it, and save your lab a fortune in buying special kits for very simple routine histochemical jobs. My ten cents-worth. June 2023 John Kiernan J. A. Kiernan MB, ChB, PhD, DSc Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Tue Jun 13 13:46:14 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Tue, 13 Jun 2023 18:46:14 +0000 Subject: [Histonet] Peroxidase Stain Message-ID: <2d9fef477a6f433d98df02fa7dde6090@holyredeemer.com> I have long used the kits from Sigma and found them to be extremely reliable. Call their tech helpline for advice, or reach out to your pathologist for their preference. I know that "homemade" saves money over kits, but for me, my most costly and precious resource is my tech time. Save yourself and your techs a headache and call Sigma-Aldrich. My 2 cents, Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Tuesday, June 13, 2023 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: [EXTERNAL] Histonet Digest, Vol 235, Issue 9 CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Peroxidase stain on peripheral smears (Kendra Beechie ND-Bismarck) 2. MOHS staining (Ken M) 3. Re: Peroxidase stain on peripheral smears (John Kiernan) ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Jun 2023 14:22:08 -0500 From: Kendra Beechie ND-Bismarck To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Message-ID: Content-Type: text/plain; charset="utf-8" Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. ------------------------------ Message: 2 Date: Mon, 12 Jun 2023 20:34:52 +0000 From: Ken M To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] MOHS staining Message-ID: Content-Type: text/plain; charset="iso-8859-1" For those doing MOHS staining either manually or automatically. How many staining cups do you really use? Are six enough if there is a running water cup nearby or do you need more? Ken ------------------------------ Message: 3 Date: Tue, 13 Jun 2023 04:46:26 +0000 From: John Kiernan To: "histonet at lists.utsouthwestern.edu" , Kendra Beechie ND-Bismarck Subject: Re: [Histonet] Peroxidase stain on peripheral smears Message-ID: Content-Type: text/plain; charset="iso-8859-1" What is the Kaplow method? I can't find it in textbooks. A quick Google search brings up only junk papers indicating that a Kaplov method may use carcinogenic benzidine (with wrong spelling) as the chromogen. There are simple, safe and inexpensive methods for histochemical localization of sites of peroxidase activity.in tissue sections or smears of cells. Buy a textbook for about $50, read it, and save your lab a fortune in buying special kits for very simple routine histochemical jobs. My ten cents-worth. June 2023 John Kiernan J. A. Kiernan MB, ChB, PhD, DSc Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________ From: Kendra Beechie ND-Bismarck via Histonet Sent: June 12, 2023 3:22 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Peroxidase stain on peripheral smears Hello, I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work. However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen.... Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with.... Any and all help would be greatly appreciated! Thank you, Kendra Beechie MLS/HTL (ASCP), Technical Consultant CHI St. Alexius Health Bismarck, ND Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 235, Issue 9 **************************************** From alapiana12 at gmail.com Tue Jun 13 13:58:20 2023 From: alapiana12 at gmail.com (Anthony Lapiana) Date: Tue, 13 Jun 2023 14:58:20 -0400 Subject: [Histonet] Histonet responses Message-ID: Good afternoon, I am new to the histonet and I am trying to figure out how to reply to people's messages they post. Also, do I get a notification when people reply to my responses or posts via email? Thanks, Anthony From jmacdonald at mtsac.edu Tue Jun 13 14:08:50 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 13 Jun 2023 19:08:50 +0000 Subject: [Histonet] Histonet responses In-Reply-To: References: Message-ID: Hi Anthony, If you hit reply only you will get a response. If you hit reply all a response will go you and the Histonet. Jennifer -----Original Message----- From: Anthony Lapiana via Histonet Sent: Tuesday, June 13, 2023 11:58 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Histonet responses EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Good afternoon, I am new to the histonet and I am trying to figure out how to reply to people's messages they post. Also, do I get a notification when people reply to my responses or posts via email? Thanks, Anthony _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Victoria_Verno at bshsi.org Wed Jun 14 10:03:02 2023 From: Victoria_Verno at bshsi.org (Verno, Victoria R.) Date: Wed, 14 Jun 2023 15:03:02 +0000 Subject: [Histonet] Biopsy formalin fixation times Message-ID: Good morning everyone, I have a question on biopsy formalin fixation time. How long should we let the biopsies set and fix in formalin before we gross/process them? You response is greatly appreciated. Victoria Verno HT ASCP Histology Supervisor of Hampton Roads Desk: 757-398-4763 Fax: 757-397-7475 CONFIDENTIALITY NOTICE: This message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From tpodawiltz at yahoo.com Wed Jun 14 10:18:03 2023 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Wed, 14 Jun 2023 15:18:03 +0000 (UTC) Subject: [Histonet] Biopsy formalin fixation times In-Reply-To: References: Message-ID: <1324643884.2241842.1686755883540@mail.yahoo.com> Too general of a question. What type of biopsy? Minimum of 6 hours, maximum of 72 hours for breast.? Sent from Yahoo Mail for iPhone On Wednesday, June 14, 2023, 11:03 AM, Verno, Victoria R. via Histonet wrote: Good morning everyone, ? ? ? ? I have a question on biopsy formalin fixation time. How long should we let the biopsies set and fix in formalin before we gross/process them? You response is greatly appreciated. Victoria Verno HT ASCP Histology Supervisor of Hampton Roads Desk: 757-398-4763 Fax: 757-397-7475 CONFIDENTIALITY NOTICE: This message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Wed Jun 14 11:41:41 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 14 Jun 2023 12:41:41 -0400 Subject: [Histonet] LIV Golf and The PGA A Lesson in Employee Retention. Message-ID: <00ec01d99edf$19703560$4c50a020$@earthlink.net> Check out my latest blog post. LIV Golf and The PGA. A lesson in employee retention. https://reliasolutionspambarker.wordpress.com/2023/06/13/liv-golf-vs-the-pga -a-lesson-in-employee-retention/ #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From Bonnie.Whitaker at osumc.edu Wed Jun 14 11:51:47 2023 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Wed, 14 Jun 2023 16:51:47 +0000 Subject: [Histonet] Biopsy formalin fixation times In-Reply-To: <1324643884.2241842.1686755883540@mail.yahoo.com> References: <1324643884.2241842.1686755883540@mail.yahoo.com> Message-ID: But with that, you can consider formalin time after grossing, and on p;rocessor to equal those times. Bonnie Whitaker AP Operations Director Office: 614-293-8418 Mobile: 614-313-0484 -----Original Message----- From: Thomas Podawiltz via Histonet Sent: Wednesday, June 14, 2023 11:18 AM To: Verno, Victoria R. ; Histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Biopsy formalin fixation times Too general of a question. What type of biopsy? Minimum of 6 hours, maximum of 72 hours for breast.? Sent from Yahoo Mail for iPhone On Wednesday, June 14, 2023, 11:03 AM, Verno, Victoria R. via Histonet wrote: Good morning everyone, ? ? ? ? I have a question on biopsy formalin fixation time. How long should we let the biopsies set and fix in formalin before we gross/process them? You response is greatly appreciated. Victoria Verno HT ASCP Histology Supervisor of Hampton Roads Desk: 757-398-4763 Fax: 757-397-7475 CONFIDENTIALITY NOTICE: This message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!CiWU23NzCMhiXieQkvkox2cvsBOvGdMWJqoU3tnKcgi9V1cqh0YHFRW7I4U1OwJLmfKqhyNgYMLdpo3ttzwv03wccodgFEi6xnDU2Q$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!CiWU23NzCMhiXieQkvkox2cvsBOvGdMWJqoU3tnKcgi9V1cqh0YHFRW7I4U1OwJLmfKqhyNgYMLdpo3ttzwv03wccodgFEi6xnDU2Q$ From alapiana12 at gmail.com Wed Jun 14 14:42:41 2023 From: alapiana12 at gmail.com (Anthony Lapiana) Date: Wed, 14 Jun 2023 15:42:41 -0400 Subject: [Histonet] Biopsy formalin fixation times Message-ID: Overnight fixation is best if possible. 12-24 hours provides an optimal environment for IHC staining, for rush cases 6 hours is recommended. It all depends on the thickness of the tissue, large excisions and breast cases require a longer fixation time. From monica.aguilera at irbbarcelona.org Thu Jun 15 01:53:04 2023 From: monica.aguilera at irbbarcelona.org (Monica Aguilera) Date: Thu, 15 Jun 2023 08:53:04 +0200 Subject: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute Message-ID: Hi! Does anyone have experience with xylene substitutes like this? https://es.vwr.com/store/product/16702338/vwr-q-path-safesolv-solvent-substitute Thanks! M?nica -- M?nica Aguilera Pujabet, DVM, PhD Senior Research Officer Histopathology Facility Institute for Research in Biomedicine - IRB Barcelona Baldiri Reixac, 10 E-08028 Barcelona - Spain Tel: +34 934033776 <%2B34%20934020546> monica.aguilera at irbbarcelona.org From adesupo2002 at hotmail.com Thu Jun 15 11:00:37 2023 From: adesupo2002 at hotmail.com (ADESUPO ADESUYI) Date: Thu, 15 Jun 2023 16:00:37 +0000 Subject: [Histonet] IHC QC Forms Message-ID: Hi, I will be glad if you guys can share your IHC QC forms with me. Sincerely, Adesupo A From alapiana12 at gmail.com Thu Jun 15 11:26:30 2023 From: alapiana12 at gmail.com (Anthony Lapiana) Date: Thu, 15 Jun 2023 12:26:30 -0400 Subject: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute Message-ID: Hey Monica, I have not used that exact substitute before, but I do currently use Sub-X from Leica. It is amazing for running down slides, but I wouldn?t recommend it for tissue processing as xylene is the best for that. Attached below Is the xylene substitute and mounting medium I use for IHC?s. https://shop.leicabiosystems.com/us/histology-consumables/reagents-solutions/pid-sub-x-clearing-medium https://www.fishersci.com/shop/products/richard-allan-scientific-mounting-medium-2/22110610 Thanks, *Lapiana, Anthony, HT(ASCP)* Laboratory Supervisor, IHC/SPU Massachusetts General Hospital From jaylundgren at gmail.com Thu Jun 15 12:07:54 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 15 Jun 2023 12:07:54 -0500 Subject: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute In-Reply-To: References: Message-ID: I've used all of them and they work fine. There's two flavors, one that smells like bug spray (aliphatic hydrocarbons based) and the citrus kind. If you have one of those people in your lab that freaks out about fragrances (which is an actual, recognized psychosis, btw, it's called hyperosmia and it can be a symptom of major psychological affective disorders), they won't be able to enter the building if you switch to a citrus based product. Your blocks will still smell like oranges after being cut and filed. But anyway, both varieties work fine. I saw the salesman for a citrus based clearing agent drink some of it to demonstrate its non-toxicity once. Neither type of xylene substitute is half as aggressive as xylene in deparaffinization or clearing, so you'll have to (roughly) double your deparaffinization and clearing times on the processor and stain line. You're going to have to write and verify all new protocols for processing and staining. The hardest part is going to be convincing your Medical Director to change. Pathologists can be some of the most hidebound, unwilling to change dinosaurs around. As far as I know, you still have to keep some xylene in your lab, unfortunately, because all the coverslipping media are either toluene or xylene based. Especially if you coverslip using an automated instrument, as most of the manufacturers specify a particular coverslipping media. So you still have to keep a fume hood/ventilation over (or even better, under) your coverslipping area. Unless you wanted to coverslip with a water soluble media, which is a pain, and not suitable for archival storage. Maybe you can dilute xylene/toluene based media with xylene substitutes? I don't know, I've never tried. You might be able to get away with it, but it would invalidate your coverslipper warranty, if you had one. There's still going to be xylene/toluene off-gassing from your drying slides. Someone tell me if they know of a non-xylene/toluene based coverslipping media, besides water based ones, of course. You didn't ask, but the formalin substitutes work fine also. They take about twice as long as formalin to fix a given specimen and are less forgiving with thick sections, fatty sections, etc. But they do work, and yield beautiful nuclear detail and prettier IHCs than 10% NBF. Again, good luck getting your pathologists to switch over. The health and safety benefits of getting rid of xylene are obvious. There might also be a financial argument for switching to a xylene substitute, as the cost of disposing of formalin and xylene in some locales is becoming prohibitive. Sincerely, Jay A. Lundgren, M.S. HTL (ASCP) On Thu, Jun 15, 2023 at 1:53?AM Monica Aguilera via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi! > > Does anyone have experience with xylene substitutes like this? > > > https://es.vwr.com/store/product/16702338/vwr-q-path-safesolv-solvent-substitute > > Thanks! > > M?nica > > -- > M?nica Aguilera Pujabet, DVM, PhD > Senior Research Officer > > Histopathology Facility > Institute for Research in Biomedicine - IRB Barcelona > Baldiri Reixac, 10 > E-08028 Barcelona - Spain > > Tel: +34 934033776 <%2B34%20934020546> > monica.aguilera at irbbarcelona.org > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jaylundgren at gmail.com Thu Jun 15 12:24:58 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 15 Jun 2023 12:24:58 -0500 Subject: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute In-Reply-To: References: Message-ID: Ay perd?n, no conoc?a que ya existe el medio de montaje Q Path? Safemount 00647520 compatible con el sustituto. Perfecto. Atentamente, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Jun 15, 2023 at 12:07?PM Jay Lundgren wrote: > I've used all of them and they work fine. There's two flavors, one that > smells like bug spray (aliphatic hydrocarbons based) and the citrus kind. > If you have one of those people in your lab that freaks out about > fragrances (which is an actual, recognized psychosis, btw, it's called > hyperosmia and it can be a symptom of major psychological affective > disorders), they won't be able to enter the building if you switch to a > citrus based product. Your blocks will still smell like oranges after > being cut and filed. > > But anyway, both varieties work fine. I saw the salesman for a citrus > based clearing agent drink some of it to demonstrate its non-toxicity once. > > Neither type of xylene substitute is half as aggressive as xylene in > deparaffinization or clearing, so you'll have to (roughly) double your > deparaffinization and clearing times on the processor and stain line. > You're going to have to write and verify all new protocols for processing > and staining. > > The hardest part is going to be convincing your Medical Director to > change. Pathologists can be some of the most hidebound, unwilling to > change dinosaurs around. > > As far as I know, you still have to keep some xylene in your lab, > unfortunately, because all the coverslipping media are either toluene or > xylene based. Especially if you coverslip using an automated instrument, > as most of the manufacturers specify a particular coverslipping media. So > you still have to keep a fume hood/ventilation over (or even better, under) > your coverslipping area. Unless you wanted to coverslip with a water > soluble media, which is a pain, and not suitable for archival storage. > Maybe you can dilute xylene/toluene based media with xylene substitutes? I > don't know, I've never tried. You might be able to get away with it, but > it would invalidate your coverslipper warranty, if you had one. There's > still going to be xylene/toluene off-gassing from your drying slides. > Someone tell me if they know of a non-xylene/toluene based coverslipping > media, besides water based ones, of course. > > You didn't ask, but the formalin substitutes work fine also. They take > about twice as long as formalin to fix a given specimen and are less > forgiving with thick sections, fatty sections, etc. But they do work, and > yield beautiful nuclear detail and prettier IHCs than 10% NBF. Again, good > luck getting your pathologists to switch over. > > > The health and safety benefits of getting rid of xylene are obvious. > There might also be a financial argument for switching to a xylene > substitute, as the cost of disposing of formalin and xylene in some locales > is becoming prohibitive. > > Sincerely, > > Jay A. Lundgren, M.S. HTL (ASCP) > > On Thu, Jun 15, 2023 at 1:53?AM Monica Aguilera via Histonet < > histonet at lists.utsouthwestern.edu> wrote: > >> Hi! >> >> Does anyone have experience with xylene substitutes like this? >> >> >> https://es.vwr.com/store/product/16702338/vwr-q-path-safesolv-solvent-substitute >> >> Thanks! >> >> M?nica >> >> -- >> M?nica Aguilera Pujabet, DVM, PhD >> Senior Research Officer >> >> Histopathology Facility >> Institute for Research in Biomedicine - IRB Barcelona >> Baldiri Reixac, 10 >> E-08028 Barcelona - Spain >> >> Tel: +34 934033776 <%2B34%20934020546> >> monica.aguilera at irbbarcelona.org >> _______________________________________________ >> Histonet mailing list >> Histonet at lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From patpxs at gmail.com Thu Jun 15 22:57:21 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Fri, 16 Jun 2023 03:57:21 +0000 (UTC) Subject: [Histonet] QIHC References: <713568283.610948.1686887841692.ref@mail.yahoo.com> Message-ID: <713568283.610948.1686887841692@mail.yahoo.com> Well......I did not pass the exam on my second attempt.? Waa! Anyway, it got me wondering:? are there statistics out there that give a break down of the pass rate of the QIHC exam? What's the percentage of HTLs that are QIHC? Sincerely, Paula Sicurello From Kim.Kolman at va.gov Fri Jun 16 09:25:31 2023 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Fri, 16 Jun 2023 14:25:31 +0000 Subject: [Histonet] losing sections Message-ID: For your entertainment, I have a bizarre phenomenon to share with the group: I am occasionally losing the TOP section on a deeper cut H&E slide. Full run of daily slides do not show this issue. A deeper slide cut later in the day might, or a deeper slide cut first thing in the morning may, when I've not run a full daily run. I have switched to a different lot number of standard slides. I have used adhesive slides. Distilled water for the water bath same as I have used for 20 years. Using Sta-On adhesive in the water bath. H&E stain done on a Prisma Plus stainer, with no changes in staining procedure. There have been NO changes to any of my procedures. This is a very random happening that is boggling my mind! If any section was going to fall off, I'd think it would be the 2nd section - (last one picked up from the water bath). I've tried to make sure the slide has thoroughly dried before putting it on the stainer. Slides appear clean, and no greasy fingerprints on the slide. The one I always lose is the TOP section/very first level taken. Any ideas? Do I just have a Histo Gremlin here? Thanks for your input. Kim Kimberly D. Kolman, HT (ASCP) Eastern Kansas Health Care System Eisenhower VA Medical Center - Histology 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 62537 Fax: 913-758-4193 From tbraud at holyredeemer.com Fri Jun 16 12:56:02 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Fri, 16 Jun 2023 17:56:02 +0000 Subject: [Histonet] Losing sections Message-ID: I may have an answer for you. Your Prisma stainer only runs the heater when needed. With full runs, the oven stays warm, but later stain runs allow for the heater to cool down. When your rack goes into the dry station, the heat comes up from the bottom to start to dry the slide, thus the bottom sections have enough dry time, but the top don't, and they wash off. We used to encounter the same problem and that is what we hypothesized was happening, because, when we ran a blank rack through 10 minutes before loading the late rack, we were fine. Or, when we dried in a 60'C oven for 15 minutes when loading that lone late rack, we were fine. Just an idea, but for us, no more wash offs of top sections. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Message: 4 Date: Fri, 16 Jun 2023 14:25:31 +0000 From: "Kolman, Kimberly D." Subject: [Histonet] losing sections For your entertainment, I have a bizarre phenomenon to share with the group: I am occasionally losing the TOP section on a deeper cut H&E slide. Full run of daily slides do not show this issue. A deeper slide cut later in the day might, or a deeper slide cut first thing in the morning may, when I've not run a full daily run. I have switched to a different lot number of standard slides. I have used adhesive slides. Distilled water for the water bath same as I have used for 20 years. Using Sta-On adhesive in the water bath. H&E stain done on a Prisma Plus stainer, with no changes in staining procedure. There have been NO changes to any of my procedures. This is a very random happening that is boggling my mind! If any section was going to fall off, I'd think it would be the 2nd section - (last one picked up from the water bath). I've tried to make sure the slide has thoroughly dried before putting it on the stainer. Slides appear clean, and no greasy fingerprints on the slide. The one I always lose is the TOP section/very first level taken. Any ideas? Do I just have a Histo Gremlin here? Thanks for your input. Kim Kimberly D. Kolman, HT (ASCP) Eastern Kansas Health Care System Eisenhower VA Medical Center - Histology 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 62537 Fax: 913-758-4193 From salbrent at sbcglobal.net Sat Jun 17 12:49:45 2023 From: salbrent at sbcglobal.net (Brent Barrett) Date: Sat, 17 Jun 2023 17:49:45 +0000 Subject: [Histonet] Histonet Digest, Vol 235, Issue 13 In-Reply-To: References: Message-ID: Unsubscribe ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, June 17, 2023 1:00:02 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 235, Issue 13 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Losing sections (Terri Braud) ---------------------------------------------------------------------- Message: 1 Date: Fri, 16 Jun 2023 17:56:02 +0000 From: "Terri Braud" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Losing sections Message-ID: Content-Type: text/plain; charset="us-ascii" I may have an answer for you. Your Prisma stainer only runs the heater when needed. With full runs, the oven stays warm, but later stain runs allow for the heater to cool down. When your rack goes into the dry station, the heat comes up from the bottom to start to dry the slide, thus the bottom sections have enough dry time, but the top don't, and they wash off. We used to encounter the same problem and that is what we hypothesized was happening, because, when we ran a blank rack through 10 minutes before loading the late rack, we were fine. Or, when we dried in a 60'C oven for 15 minutes when loading that lone late rack, we were fine. Just an idea, but for us, no more wash offs of top sections. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Message: 4 Date: Fri, 16 Jun 2023 14:25:31 +0000 From: "Kolman, Kimberly D." Subject: [Histonet] losing sections For your entertainment, I have a bizarre phenomenon to share with the group: I am occasionally losing the TOP section on a deeper cut H&E slide. Full run of daily slides do not show this issue. A deeper slide cut later in the day might, or a deeper slide cut first thing in the morning may, when I've not run a full daily run. I have switched to a different lot number of standard slides. I have used adhesive slides. Distilled water for the water bath same as I have used for 20 years. Using Sta-On adhesive in the water bath. H&E stain done on a Prisma Plus stainer, with no changes in staining procedure. There have been NO changes to any of my procedures. This is a very random happening that is boggling my mind! If any section was going to fall off, I'd think it would be the 2nd section - (last one picked up from the water bath). I've tried to make sure the slide has thoroughly dried before putting it on the stainer. Slides appear clean, and no greasy fingerprints on the slide. The one I always lose is the TOP section/very first level taken. Any ideas? Do I just have a Histo Gremlin here? Thanks for your input. Kim Kimberly D. Kolman, HT (ASCP) Eastern Kansas Health Care System Eisenhower VA Medical Center - Histology 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 62537 Fax: 913-758-4193 ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ End of Histonet Digest, Vol 235, Issue 13 ***************************************** From Nancy.Schmitt at mercyhealth.com Mon Jun 19 10:29:15 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Mon, 19 Jun 2023 15:29:15 +0000 Subject: [Histonet] claudin 18.2 Message-ID: Hello- Where are you sending Claudin 18.2 testing? Thanks Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor MercyOne Dubuque Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From mwalker at vdxpathology.com Mon Jun 19 14:45:31 2023 From: mwalker at vdxpathology.com (Melanie Walker) Date: Mon, 19 Jun 2023 19:45:31 +0000 Subject: [Histonet] Temperature Monitoring Questions Message-ID: <0f9e97273f3747fc93ef4d7de5d44a50@vdxpathology.com> Dear Histonetters, We are considering overhauling our temperature monitoring SOP for GLP preclinical veterinary histology laboratory and would like to give ourselves some leeway with minor / short term temperature excursions. I recently attended a workshop wherein it was mentioned that you could include acceptable excursions into your SOPs. Currently we are writing a lot of deviation reports for minor excursions. I would love some opinions on temperature excursions in a GLP (not GMP/CAP) histology lab environment. We do not store temperature dependent study materials such as test articles / drugs / fresh or frozen tissues, etc., only staining reagents. Is it acceptable to state that since stain reagents are regularly removed from the refrigerated environment during staining use for up to an 8 hour shift, that excursions up to room temperature for a period of less than 8 hours are acceptable? How about if we include the language that each staining run will be completed with a known positive control to demonstrate continued effectiveness of the staining reagents? What about excursions below standard fridge temp of 2-8C? Anything above freezing OK? Does the use of a known positive control with each run effectively negate the need to report excursions? Would the same logic apply to IHC reagents that are stored at fridge temperatures? Similarly if a -20 freezer is still within freezing temp would that be acceptable - right now we have a +/- 3C range on the -20 storage but we are really just trying to avoid freeze / thaw cycles right? Going down to -12 C for a couple hours shouldn't be detrimental... Similarly, we are monitoring the room temperature of the histology laboratory but have noted previously that sometimes in the summer the afterhours temps can sometimes be OOS (the AC can only work so hard!) - since work is not being performed during this time there is no detrimental effect on sectioning / embedding quality and reagents are tested upon each use with known controls, can we include some language to that effect to reduce the number of deviations reports for OOS temps? Is it required to have a NIST certified thermometer / calibrate against such if we are not manufacturing nor storing drugs / test articles / etc.? Can we use another acceptable accredited calibration standard (i.e., ISO/IEC 17025 accredited calibration laboratory)? Is this necessary for our purposes? Also, we recently acquired data logging thermometers with alarm capability - the procedure would move from daily tech review of each thermometer to monthly management review of the data (along with of course addressing any OOS situation that sets off the alarms in the moment). We would log the monthly review as well as retain the data printouts for facility records. Any concerns with this approach? I think one concern is that if they set the alarm to go off below the stated acceptable range they will need to produce a deviation however if they set it above so it notifies them before an OOS has occurred they are dealing with alarm situations when the temp is still within range... how do others handle this? Any additional advice/ feedback regarding monitoring fridge / freezer / lab space temps as pertains to histology staining / processing would be much appreciated! Sorry for the million questions! Thanks in advance! From jmacdonald at mtsac.edu Mon Jun 19 16:29:35 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 19 Jun 2023 21:29:35 +0000 Subject: [Histonet] UCSD clinical lab is hiring Histotechnologist II and a Hospital Lab Technician In-Reply-To: <1276958700.248882.1686844721999@mail.yahoo.com> References: <1276958700.248882.1686844721999.ref@mail.yahoo.com> <1276958700.248882.1686844721999@mail.yahoo.com> Message-ID: My lab is currently looking to hire 1 Histotech and 1 Hospital Lab Tech. New graduates are very welcome to apply. Willing to train. If you have any questions or would like to know more, please contact Sabrina at: ssy at health.ucsd.edu. Thank you! Regards, Sabrina HISTOTECHNOLOGIST II - PATHOLOGY LAB - 123529 Histotechnologist II - Pathology Lab - 123529 [https://s.yimg.com/lo/api/res/1.2/AnxGW.mgGRAhdx0wUh4jAQ--~A/Zmk9ZmlsbDt3PTgwO2g9ODA7YXBwaWQ9aWV4dHJhY3Q-/https:/dy5f5j6i37p1a.cloudfront.net/facebook/jobshare/e4cad3b0d54e11ebb013e8315006bed8.png.cf.jpg] Histotechnologist II - Pathology Lab - ... HOSPITAL LAB TECHNICIAN I - PATHOLOGY LAB - 123527 Hospital Lab Technician I - Pathology Lab - 123527 [https://s.yimg.com/lo/api/res/1.2/AnxGW.mgGRAhdx0wUh4jAQ--~A/Zmk9ZmlsbDt3PTgwO2g9ODA7YXBwaWQ9aWV4dHJhY3Q-/https:/dy5f5j6i37p1a.cloudfront.net/facebook/jobshare/e4cad3b0d54e11ebb013e8315006bed8.png.cf.jpg] Hospital Lab Technician I - Pathology L... From Kim.Kolman at va.gov Tue Jun 20 09:52:07 2023 From: Kim.Kolman at va.gov (Kolman, Kimberly D.) Date: Tue, 20 Jun 2023 14:52:07 +0000 Subject: [Histonet] Losing sections In-Reply-To: References: Message-ID: Terri this sounds entirely logical! I will try running a blank rack to warm the drying station up before running a single deeper slide. My pathologist and I will both be very happy if it makes a difference! Thank you! -----Original Message----- From: Terri Braud Sent: Friday, June 16, 2023 12:56 PM To: histonet at lists.utsouthwestern.edu Cc: Kolman, Kimberly D. Subject: [EXTERNAL] RE: Losing sections I may have an answer for you. Your Prisma stainer only runs the heater when needed. With full runs, the oven stays warm, but later stain runs allow for the heater to cool down. When your rack goes into the dry station, the heat comes up from the bottom to start to dry the slide, thus the bottom sections have enough dry time, but the top don't, and they wash off. We used to encounter the same problem and that is what we hypothesized was happening, because, when we ran a blank rack through 10 minutes before loading the late rack, we were fine. Or, when we dried in a 60'C oven for 15 minutes when loading that lone late rack, we were fine. Just an idea, but for us, no more wash offs of top sections. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Message: 4 Date: Fri, 16 Jun 2023 14:25:31 +0000 From: "Kolman, Kimberly D." Subject: [Histonet] losing sections For your entertainment, I have a bizarre phenomenon to share with the group: I am occasionally losing the TOP section on a deeper cut H&E slide. Full run of daily slides do not show this issue. A deeper slide cut later in the day might, or a deeper slide cut first thing in the morning may, when I've not run a full daily run. I have switched to a different lot number of standard slides. I have used adhesive slides. Distilled water for the water bath same as I have used for 20 years. Using Sta-On adhesive in the water bath. H&E stain done on a Prisma Plus stainer, with no changes in staining procedure. There have been NO changes to any of my procedures. This is a very random happening that is boggling my mind! If any section was going to fall off, I'd think it would be the 2nd section - (last one picked up from the water bath). I've tried to make sure the slide has thoroughly dried before putting it on the stainer. Slides appear clean, and no greasy fingerprints on the slide. The one I always lose is the TOP section/very first level taken. Any ideas? Do I just have a Histo Gremlin here? Thanks for your input. Kim Kimberly D. Kolman, HT (ASCP) Eastern Kansas Health Care System Eisenhower VA Medical Center - Histology 115 4101 S. 4th St. Trfwy. Leavenworth, KS 66048 913-682-2000 x 62537 Fax: 913-758-4193 From PREISZNE at mail.etsu.edu Tue Jun 20 15:15:17 2023 From: PREISZNE at mail.etsu.edu (Preiszner, Johanna) Date: Tue, 20 Jun 2023 20:15:17 +0000 Subject: [Histonet] IHC wash buffer temperature? Message-ID: Hello Histonetters, I work in research and we get too much non-specific background on our IHC-s. Anybody tried using wash solution at 37C instead of at room temperature? If not, why? Is it a bad idea? Thanks, Hanna Preiszner ETSU Pathology From jmacdonald at mtsac.edu Tue Jun 20 16:03:06 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Tue, 20 Jun 2023 21:03:06 +0000 Subject: [Histonet] Fountain Valley Histology technician In-Reply-To: <000d01d99ef1$f955b170$ec011450$@biopath.org> References: <000d01d99ef1$f955b170$ec011450$@biopath.org> Message-ID: Bio-Path has an open position for a histotechnician. If interested or if you have any questions about the position, please contact Paula Lucas at plucas at biopath.org From David.Burk at pbrc.edu Wed Jun 21 16:48:40 2023 From: David.Burk at pbrc.edu (David Burk) Date: Wed, 21 Jun 2023 21:48:40 +0000 Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features Message-ID: We are working out an alternative method for collagen staining using Fast Green / Sirius Red (Direct Red 80) rather than the standard picrosirius red method as I think it is prettier and easier to see the collagen on a green background. What we?ve noticed, though, is that we are seeing intermediate blue staining in the tissue in particular regions or structures. I?ve not had any success in finding an explanation for this online and was hoping someone on the board may have an idea of what?s going on and what is being stained blue in our tissue sections. We have examined a variety of murine or rat tissues including liver, heart, kidney, lung, skeletal muscle, spleen, brain, pancreas, and even decellularized human adipose tissue. There are, almost always, some structures/features that exhibit a denim blue to lighter blue-green color (at least to my eye) in addition to the expected red-colored structures that we would assume to be collagen, light green cytoplasm, and yellow-ish features stained with picric acid. An interesting tidbit is that these blue-ish stained features are birefringent under polarized light so you would not know their color (with transmitted imaging) was atypical. I don?t want to use a stain if I can?t let people know what a particular color represents and can also cause problems with the quantification of collagen using a color-based approach. Our protocol is as follows: 1. Dewax 2. H2O rinse 3. Stain in a 0.1% Fast Green FCF (C.I. 42053) and 0.1% Direct Red 80 (C.I. 35780) solution dissolved in saturated picric acid for 1 hour at room temperature 4. Dip 5x and then immerse in 0.5% acetic acid for 5 seconds 5. Repeat step 4 6. Dip 5x and then immerse in 100% Ethanol 30 seconds 7. Dehydrate in 100% Ethanol 1 min 8. Repeat step 7 9. 3 x Xylene for 2 min each 10. Coverslip I?m uploading some images from mouse muscle and tumor tissue to the Histonet Image upload site. If that doesn?t work, here are links: Mouse tumor: https://drive.google.com/file/d/1VjOZFzvsQByQLuDtdGPfdwAwap_CVE58/view?usp=sharing Mouse skeletal muscle: https://drive.google.com/file/d/10vT_FKu3-Ad5uemM5gnZmqDCKb3fs2lV/view?usp=sharing Thanks, David Burk From jkiernan at uwo.ca Wed Jun 21 23:36:05 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 22 Jun 2023 04:36:05 +0000 Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features In-Reply-To: References: Message-ID: Your technique is the one first (I think) published by Lopez-De Leon A & Rojkind M (1985) A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J. Histochem. Cytochem. 33: 737-743. The photos in that paper show some of the collagen almost black - surely taking up both red and green dyes. More recent papers describe exactly the same method, and there are also some variants. Your technique, with an acid rinse after staining for an hour, then quick transition to rapid dehydration in 100% alcohol, is essential for any valid picro-sirius staining. According to the entry for fast green FCF (CI 42053) in Conn's Biological Stains (10th ed, p.180-182), "chemically distinct blue-green dyes have been supplied under this name". Are you sure your fast green FCF is the real McCoy? Is it from a batch certified by the Biological Stain Commission? The jar of dye powder should have a small bluish label, with features that make forgery difficult. See https://biologicalstaincommission.org/certified-biological-dyesstains/ for pictures and other information. There are companies selling "certified stains" that have not been tested and certified by the Biological Stain Commission. Caveat emptor! The Biological Stain Commission is a not-for-profit corporation that has been providing third-party quality control and other services for vendors and users of stains for 100 years. Just a few thoughts; I could add more, but probably this letter already is too long for the Histonet censors. John Kiernan Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: David Burk via Histonet Sent: June 21, 2023 5:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features We are working out an alternative method for collagen staining using Fast Green / Sirius Red (Direct Red 80) rather than the standard picrosirius red method as I think it is prettier and easier to see the collagen on a green background. What we?ve noticed, though, is that we are seeing intermediate blue staining in the tissue in particular regions or structures. I?ve not had any success in finding an explanation for this online and was hoping someone on the board may have an idea of what?s going on and what is being stained blue in our tissue sections. We have examined a variety of murine or rat tissues including liver, heart, kidney, lung, skeletal muscle, spleen, brain, pancreas, and even decellularized human adipose tissue. There are, almost always, some structures/features that exhibit a denim blue to lighter blue-green color (at least to my eye) in addition to the expected red-colored structures that we would assume to be collagen, light green cytoplasm, and yellow-ish features stained with picric acid. An interesting tidbit is that these blue-ish stained features are birefringent under polarized light so you would not know their color (with transmitted imaging) was atypical. I don?t want to use a stain if I can?t let people know what a particular color represents and can also cause problems with the quantification of collagen using a color-based approach. Our protocol is as follows: 1. Dewax 2. H2O rinse 3. Stain in a 0.1% Fast Green FCF (C.I. 42053) and 0.1% Direct Red 80 (C.I. 35780) solution dissolved in saturated picric acid for 1 hour at room temperature 4. Dip 5x and then immerse in 0.5% acetic acid for 5 seconds 5. Repeat step 4 6. Dip 5x and then immerse in 100% Ethanol 30 seconds 7. Dehydrate in 100% Ethanol 1 min 8. Repeat step 7 9. 3 x Xylene for 2 min each 10. Coverslip I?m uploading some images from mouse muscle and tumor tissue to the Histonet Image upload site. If that doesn?t work, here are links: Mouse tumor: https://drive.google.com/file/d/1VjOZFzvsQByQLuDtdGPfdwAwap_CVE58/view?usp=sharing Mouse skeletal muscle: https://drive.google.com/file/d/10vT_FKu3-Ad5uemM5gnZmqDCKb3fs2lV/view?usp=sharing Thanks, David Burk _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Burk at pbrc.edu Thu Jun 22 07:37:52 2023 From: David.Burk at pbrc.edu (David Burk) Date: Thu, 22 Jun 2023 12:37:52 +0000 Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features In-Reply-To: References: Message-ID: Dr. Kiernan, You are correct regarding the source of the technique we are emulating. We have had issues with consistency in keeping the yellow picric acid color in cytoplasm when performing the traditional PSR stain possibly due to variability in what each individual considers 'quick' in their rinses and ethanol steps. Looking in the literature, you can find many publications that show PRS-stained tissue with golden-yellow / straw-colored cytoplasm (ideal), pale pink, or even virtually 'clear'. The stain we are currently using is from a bottle likely purchased in the 80's or 90's and does bear the label you describe. Allied Chemical in Morristown, NJ which no longer exists. I'll try a newer bottle we have and see if the results remain the same. Our Direct Red 80 is from Sigma-Aldrich (365548) and does not bear the certification mark you mention. However, as we do see the expected collagen staining in tissues (and the stained material exhibits birefringence), I am fairly confident that it is, at the very least, 'OK'. If you happen to know of vendors that routinely offer certified dyes, I'd be happy to utilize them in the future. As you mention in your separate email, what I may consider blue may appear differently to others either by technology or anatomy/physiology. Regardless, they are unexpected and have not been described in the literature and, in that regard, present a potential source of confusion. Best, David ________________________________ From: John Kiernan Sent: Wednesday, June 21, 2023 11:36 PM To: histonet at lists.utsouthwestern.edu ; David Burk Subject: Re: Fast Green / Sirius Red - Unknown blue features Your technique is the one first (I think) published by Lopez-De Leon A & Rojkind M (1985) A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J. Histochem. Cytochem. 33: 737-743. The photos in that paper show some of the collagen almost black - surely taking up both red and green dyes. More recent papers describe exactly the same method, and there are also some variants. Your technique, with an acid rinse after staining for an hour, then quick transition to rapid dehydration in 100% alcohol, is essential for any valid picro-sirius staining. According to the entry for fast green FCF (CI 42053) in Conn's Biological Stains (10th ed, p.180-182), "chemically distinct blue-green dyes have been supplied under this name". Are you sure your fast green FCF is the real McCoy? Is it from a batch certified by the Biological Stain Commission? The jar of dye powder should have a small bluish label, with features that make forgery difficult. See https://biologicalstaincommission.org/certified-biological-dyesstains/ for pictures and other information. There are companies selling "certified stains" that have not been tested and certified by the Biological Stain Commission. Caveat emptor! The Biological Stain Commission is a not-for-profit corporation that has been providing third-party quality control and other services for vendors and users of stains for 100 years. Just a few thoughts; I could add more, but probably this letter already is too long for the Histonet censors. John Kiernan Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ From: David Burk via Histonet Sent: June 21, 2023 5:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features We are working out an alternative method for collagen staining using Fast Green / Sirius Red (Direct Red 80) rather than the standard picrosirius red method as I think it is prettier and easier to see the collagen on a green background. What we?ve noticed, though, is that we are seeing intermediate blue staining in the tissue in particular regions or structures. I?ve not had any success in finding an explanation for this online and was hoping someone on the board may have an idea of what?s going on and what is being stained blue in our tissue sections. We have examined a variety of murine or rat tissues including liver, heart, kidney, lung, skeletal muscle, spleen, brain, pancreas, and even decellularized human adipose tissue. There are, almost always, some structures/features that exhibit a denim blue to lighter blue-green color (at least to my eye) in addition to the expected red-colored structures that we would assume to be collagen, light green cytoplasm, and yellow-ish features stained with picric acid. An interesting tidbit is that these blue-ish stained features are birefringent under polarized light so you would not know their color (with transmitted imaging) was atypical. I don?t want to use a stain if I can?t let people know what a particular color represents and can also cause problems with the quantification of collagen using a color-based approach. Our protocol is as follows: 1. Dewax 2. H2O rinse 3. Stain in a 0.1% Fast Green FCF (C.I. 42053) and 0.1% Direct Red 80 (C.I. 35780) solution dissolved in saturated picric acid for 1 hour at room temperature 4. Dip 5x and then immerse in 0.5% acetic acid for 5 seconds 5. Repeat step 4 6. Dip 5x and then immerse in 100% Ethanol 30 seconds 7. Dehydrate in 100% Ethanol 1 min 8. Repeat step 7 9. 3 x Xylene for 2 min each 10. Coverslip I?m uploading some images from mouse muscle and tumor tissue to the Histonet Image upload site. If that doesn?t work, here are links: Mouse tumor: https://drive.google.com/file/d/1VjOZFzvsQByQLuDtdGPfdwAwap_CVE58/view?usp=sharing Mouse skeletal muscle: https://drive.google.com/file/d/10vT_FKu3-Ad5uemM5gnZmqDCKb3fs2lV/view?usp=sharing Thanks, David Burk _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz at premierlab.com Thu Jun 22 09:36:57 2023 From: liz at premierlab.com (Liz Chlipala) Date: Thu, 22 Jun 2023 14:36:57 +0000 Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features In-Reply-To: References: Message-ID: David We have found that mordanting in bouins (just like you would a Masson's trichrome) prior to completing a standard Picrosirius Red stain protocol that we get very consistent results. I think this method has been published but I do not have access to the reference currently. Its worth a shot for your PSR stain. What I do know is that the brief rinse in acetic acid post the PSR stain is necessary, that seems to remove any excess red/pink that may stain the cytoplasm of the cells, not much of a distractor for manual reads for fibrosis but may be problematic if you are utilizing an image analysis solution. I can't comment on the other method. If you like I can forward on our PSR method in a separate email. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com ? Take a look at our most recent publications: https://journals.lww.com/appliedimmunohist/Abstract/publishahead/An_Image_Analysis_Solution_For_Quantification_and.98711.aspx https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879147/ -----Original Message----- From: David Burk via Histonet Sent: Thursday, June 22, 2023 6:38 AM To: John Kiernan ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Fast Green / Sirius Red - Unknown blue features Dr. Kiernan, You are correct regarding the source of the technique we are emulating. We have had issues with consistency in keeping the yellow picric acid color in cytoplasm when performing the traditional PSR stain possibly due to variability in what each individual considers 'quick' in their rinses and ethanol steps. Looking in the literature, you can find many publications that show PRS-stained tissue with golden-yellow / straw-colored cytoplasm (ideal), pale pink, or even virtually 'clear'. The stain we are currently using is from a bottle likely purchased in the 80's or 90's and does bear the label you describe. Allied Chemical in Morristown, NJ which no longer exists. I'll try a newer bottle we have and see if the results remain the same. Our Direct Red 80 is from Sigma-Aldrich (365548) and does not bear the certification mark you mention. However, as we do see the expected collagen staining in tissues (and the stained material exhibits birefringence), I am fairly confident that it is, at the very least, 'OK'. If you happen to know of vendors that routinely offer certified dyes, I'd be happy to utilize them in the future. As you mention in your separate email, what I may consider blue may appear differently to others either by technology or anatomy/physiology. Regardless, they are unexpected and have not been described in the literature and, in that regard, present a potential source of confusion. Best, David ________________________________ From: John Kiernan Sent: Wednesday, June 21, 2023 11:36 PM To: histonet at lists.utsouthwestern.edu ; David Burk Subject: Re: Fast Green / Sirius Red - Unknown blue features Your technique is the one first (I think) published by Lopez-De Leon A & Rojkind M (1985) A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J. Histochem. Cytochem. 33: 737-743. The photos in that paper show some of the collagen almost black - surely taking up both red and green dyes. More recent papers describe exactly the same method, and there are also some variants. Your technique, with an acid rinse after staining for an hour, then quick transition to rapid dehydration in 100% alcohol, is essential for any valid picro-sirius staining. According to the entry for fast green FCF (CI 42053) in Conn's Biological Stains (10th ed, p.180-182), "chemically distinct blue-green dyes have been supplied under this name". Are you sure your fast green FCF is the real McCoy? Is it from a batch certified by the Biological Stain Commission? The jar of dye powder should have a small bluish label, with features that make forgery difficult. See https://us-west-2.protection.sophos.com?d=biologicalstaincommission.org&u=aHR0cHM6Ly9iaW9sb2dpY2Fsc3RhaW5jb21taXNzaW9uLm9yZy9jZXJ0aWZpZWQtYmlvbG9naWNhbC1keWVzc3RhaW5zLw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=WjVWeGtKVHl5R3FneU9tQjkxK1gydnlWY2Y4Yk9ESGZ1SjBkY1c3ZmxRST0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k for pictures and other information. There are companies selling "certified stains" that have not been tested and certified by the Biological Stain Commission. Caveat emptor! The Biological Stain Commission is a not-for-profit corporation that has been providing third-party quality control and other services for vendors and users of stains for 100 years. Just a few thoughts; I could add more, but probably this letter already is too long for the Histonet censors. John Kiernan Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://us-west-2.protection.sophos.com?d=uwo.ca&u=aHR0cHM6Ly93d3cuc2NodWxpY2gudXdvLmNhL2FuYXRvbXkvcGVvcGxlL2Jpb3MvZW1lcml0aS9raWVybmFuX2pvaG4uaHRtbA==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=NjhVVUJSWDJ5TlVOTHJ0QmgyTlNjd0dzK0ZUV1kyVEFHMnJlMmVLbS82ST0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Also Secretary, Biological Stain Commission, Inc. https://us-west-2.protection.sophos.com?d=biologicalstaincommission.org&u=aHR0cHM6Ly9iaW9sb2dpY2Fsc3RhaW5jb21taXNzaW9uLm9yZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=VlpjOEZ2cXZPQ3hCeGxVMTJqbWExbnlyemQ1alREREhnK3dYMk5WMy80dz0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k = = = ________________________________ From: David Burk via Histonet Sent: June 21, 2023 5:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features We are working out an alternative method for collagen staining using Fast Green / Sirius Red (Direct Red 80) rather than the standard picrosirius red method as I think it is prettier and easier to see the collagen on a green background. What we've noticed, though, is that we are seeing intermediate blue staining in the tissue in particular regions or structures. I've not had any success in finding an explanation for this online and was hoping someone on the board may have an idea of what's going on and what is being stained blue in our tissue sections. We have examined a variety of murine or rat tissues including liver, heart, kidney, lung, skeletal muscle, spleen, brain, pancreas, and even decellularized human adipose tissue. There are, almost always, some structures/features that exhibit a denim blue to lighter blue-green color (at least to my eye) in addition to the expected red-colored structures that we would assume to be collagen, light green cytoplasm, and yellow-ish features stained with picric acid. An interesting tidbit is that these blue-ish stained features are birefringent under polarized light so you would not know their color (with transmitted imaging) was atypical. I don't want to use a stain if I can't let people know what a particular color represents and can also cause problems with the quantification of collagen using a color-based approach. Our protocol is as follows: 1. Dewax 2. H2O rinse 3. Stain in a 0.1% Fast Green FCF (C.I. 42053) and 0.1% Direct Red 80 (C.I. 35780) solution dissolved in saturated picric acid for 1 hour at room temperature 4. Dip 5x and then immerse in 0.5% acetic acid for 5 seconds 5. Repeat step 4 6. Dip 5x and then immerse in 100% Ethanol 30 seconds 7. Dehydrate in 100% Ethanol 1 min 8. Repeat step 7 9. 3 x Xylene for 2 min each 10. Coverslip I'm uploading some images from mouse muscle and tumor tissue to the Histonet Image upload site. If that doesn't work, here are links: Mouse tumor: https://us-west-2.protection.sophos.com?d=google.com&u=aHR0cHM6Ly9kcml2ZS5nb29nbGUuY29tL2ZpbGUvZC8xVmpPWkZ6dnNRQnlRTHVEdGRHUGZkd0F3YXBfQ1ZFNTgvdmlldz91c3A9c2hhcmluZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=dlN6YnhXQXI0UHBZSVRCb2JxY1hLU054WHZwUGtoc2xXZWN4eUpoUXN4cz0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Mouse skeletal muscle: https://us-west-2.protection.sophos.com?d=google.com&u=aHR0cHM6Ly9kcml2ZS5nb29nbGUuY29tL2ZpbGUvZC8xMHZUX0ZLdTMtQWQ1dWVtTTVnblptcURDS2IzZnMybFYvdmlldz91c3A9c2hhcmluZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=OXpFOVZvNzV4cDYvN2R6bkhiNFh3SFAyaHBTc3NsTDJYOFFaZTBTRDI3OD0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Thanks, David Burk _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://us-west-2.protection.sophos.com?d=utsouthwestern.edu&u=aHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=d09mYk52YVVMZ0lDQTdMZTV5SDZCbHhPdWY1elBXcTNBNTd1cWRjSER0TT0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://us-west-2.protection.sophos.com?d=utsouthwestern.edu&u=aHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=d09mYk52YVVMZ0lDQTdMZTV5SDZCbHhPdWY1elBXcTNBNTd1cWRjSER0TT0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k From David.Burk at pbrc.edu Thu Jun 22 10:20:38 2023 From: David.Burk at pbrc.edu (David Burk) Date: Thu, 22 Jun 2023 15:20:38 +0000 Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features In-Reply-To: References: Message-ID: Thanks, Liz. We have not tried that for PSR before but could easily be tested. Thanks for sending the protocol! David ________________________________ From: Liz Chlipala Sent: Thursday, June 22, 2023 9:36 AM To: David Burk ; John Kiernan ; histonet at lists.utsouthwestern.edu Subject: RE: Fast Green / Sirius Red - Unknown blue features David We have found that mordanting in bouins (just like you would a Masson's trichrome) prior to completing a standard Picrosirius Red stain protocol that we get very consistent results. I think this method has been published but I do not have access to the reference currently. Its worth a shot for your PSR stain. What I do know is that the brief rinse in acetic acid post the PSR stain is necessary, that seems to remove any excess red/pink that may stain the cytoplasm of the cells, not much of a distractor for manual reads for fibrosis but may be problematic if you are utilizing an image analysis solution. I can't comment on the other method. If you like I can forward on our PSR method in a separate email. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell liz at premierlab.com www.premierlab.com Take a look at our most recent publications: https://journals.lww.com/appliedimmunohist/Abstract/publishahead/An_Image_Analysis_Solution_For_Quantification_and.98711.aspx https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6879147/ -----Original Message----- From: David Burk via Histonet Sent: Thursday, June 22, 2023 6:38 AM To: John Kiernan ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Fast Green / Sirius Red - Unknown blue features Dr. Kiernan, You are correct regarding the source of the technique we are emulating. We have had issues with consistency in keeping the yellow picric acid color in cytoplasm when performing the traditional PSR stain possibly due to variability in what each individual considers 'quick' in their rinses and ethanol steps. Looking in the literature, you can find many publications that show PRS-stained tissue with golden-yellow / straw-colored cytoplasm (ideal), pale pink, or even virtually 'clear'. The stain we are currently using is from a bottle likely purchased in the 80's or 90's and does bear the label you describe. Allied Chemical in Morristown, NJ which no longer exists. I'll try a newer bottle we have and see if the results remain the same. Our Direct Red 80 is from Sigma-Aldrich (365548) and does not bear the certification mark you mention. However, as we do see the expected collagen staining in tissues (and the stained material exhibits birefringence), I am fairly confident that it is, at the very least, 'OK'. If you happen to know of vendors that routinely offer certified dyes, I'd be happy to utilize them in the future. As you mention in your separate email, what I may consider blue may appear differently to others either by technology or anatomy/physiology. Regardless, they are unexpected and have not been described in the literature and, in that regard, present a potential source of confusion. Best, David ________________________________ From: John Kiernan Sent: Wednesday, June 21, 2023 11:36 PM To: histonet at lists.utsouthwestern.edu ; David Burk Subject: Re: Fast Green / Sirius Red - Unknown blue features Your technique is the one first (I think) published by Lopez-De Leon A & Rojkind M (1985) A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J. Histochem. Cytochem. 33: 737-743. The photos in that paper show some of the collagen almost black - surely taking up both red and green dyes. More recent papers describe exactly the same method, and there are also some variants. Your technique, with an acid rinse after staining for an hour, then quick transition to rapid dehydration in 100% alcohol, is essential for any valid picro-sirius staining. According to the entry for fast green FCF (CI 42053) in Conn's Biological Stains (10th ed, p.180-182), "chemically distinct blue-green dyes have been supplied under this name". Are you sure your fast green FCF is the real McCoy? Is it from a batch certified by the Biological Stain Commission? The jar of dye powder should have a small bluish label, with features that make forgery difficult. See https://us-west-2.protection.sophos.com?d=biologicalstaincommission.org&u=aHR0cHM6Ly9iaW9sb2dpY2Fsc3RhaW5jb21taXNzaW9uLm9yZy9jZXJ0aWZpZWQtYmlvbG9naWNhbC1keWVzc3RhaW5zLw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=WjVWeGtKVHl5R3FneU9tQjkxK1gydnlWY2Y4Yk9ESGZ1SjBkY1c3ZmxRST0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k for pictures and other information. There are companies selling "certified stains" that have not been tested and certified by the Biological Stain Commission. Caveat emptor! The Biological Stain Commission is a not-for-profit corporation that has been providing third-party quality control and other services for vendors and users of stains for 100 years. Just a few thoughts; I could add more, but probably this letter already is too long for the Histonet censors. John Kiernan Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://us-west-2.protection.sophos.com?d=uwo.ca&u=aHR0cHM6Ly93d3cuc2NodWxpY2gudXdvLmNhL2FuYXRvbXkvcGVvcGxlL2Jpb3MvZW1lcml0aS9raWVybmFuX2pvaG4uaHRtbA==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=NjhVVUJSWDJ5TlVOTHJ0QmgyTlNjd0dzK0ZUV1kyVEFHMnJlMmVLbS82ST0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Also Secretary, Biological Stain Commission, Inc. https://us-west-2.protection.sophos.com?d=biologicalstaincommission.org&u=aHR0cHM6Ly9iaW9sb2dpY2Fsc3RhaW5jb21taXNzaW9uLm9yZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=VlpjOEZ2cXZPQ3hCeGxVMTJqbWExbnlyemQ1alREREhnK3dYMk5WMy80dz0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k = = = ________________________________ From: David Burk via Histonet Sent: June 21, 2023 5:48 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Fast Green / Sirius Red - Unknown blue features We are working out an alternative method for collagen staining using Fast Green / Sirius Red (Direct Red 80) rather than the standard picrosirius red method as I think it is prettier and easier to see the collagen on a green background. What we've noticed, though, is that we are seeing intermediate blue staining in the tissue in particular regions or structures. I've not had any success in finding an explanation for this online and was hoping someone on the board may have an idea of what's going on and what is being stained blue in our tissue sections. We have examined a variety of murine or rat tissues including liver, heart, kidney, lung, skeletal muscle, spleen, brain, pancreas, and even decellularized human adipose tissue. There are, almost always, some structures/features that exhibit a denim blue to lighter blue-green color (at least to my eye) in addition to the expected red-colored structures that we would assume to be collagen, light green cytoplasm, and yellow-ish features stained with picric acid. An interesting tidbit is that these blue-ish stained features are birefringent under polarized light so you would not know their color (with transmitted imaging) was atypical. I don't want to use a stain if I can't let people know what a particular color represents and can also cause problems with the quantification of collagen using a color-based approach. Our protocol is as follows: 1. Dewax 2. H2O rinse 3. Stain in a 0.1% Fast Green FCF (C.I. 42053) and 0.1% Direct Red 80 (C.I. 35780) solution dissolved in saturated picric acid for 1 hour at room temperature 4. Dip 5x and then immerse in 0.5% acetic acid for 5 seconds 5. Repeat step 4 6. Dip 5x and then immerse in 100% Ethanol 30 seconds 7. Dehydrate in 100% Ethanol 1 min 8. Repeat step 7 9. 3 x Xylene for 2 min each 10. Coverslip I'm uploading some images from mouse muscle and tumor tissue to the Histonet Image upload site. If that doesn't work, here are links: Mouse tumor: https://us-west-2.protection.sophos.com?d=google.com&u=aHR0cHM6Ly9kcml2ZS5nb29nbGUuY29tL2ZpbGUvZC8xVmpPWkZ6dnNRQnlRTHVEdGRHUGZkd0F3YXBfQ1ZFNTgvdmlldz91c3A9c2hhcmluZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=dlN6YnhXQXI0UHBZSVRCb2JxY1hLU054WHZwUGtoc2xXZWN4eUpoUXN4cz0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Mouse skeletal muscle: https://us-west-2.protection.sophos.com?d=google.com&u=aHR0cHM6Ly9kcml2ZS5nb29nbGUuY29tL2ZpbGUvZC8xMHZUX0ZLdTMtQWQ1dWVtTTVnblptcURDS2IzZnMybFYvdmlldz91c3A9c2hhcmluZw==&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=OXpFOVZvNzV4cDYvN2R6bkhiNFh3SFAyaHBTc3NsTDJYOFFaZTBTRDI3OD0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k Thanks, David Burk _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://us-west-2.protection.sophos.com?d=utsouthwestern.edu&u=aHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=d09mYk52YVVMZ0lDQTdMZTV5SDZCbHhPdWY1elBXcTNBNTd1cWRjSER0TT0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://us-west-2.protection.sophos.com?d=utsouthwestern.edu&u=aHR0cDovL2xpc3RzLnV0c291dGh3ZXN0ZXJuLmVkdS9tYWlsbWFuL2xpc3RpbmZvL2hpc3RvbmV0&i=NWYzYTk2OTEyMDVmMDkwZWJiNmJlNzc2&t=d09mYk52YVVMZ0lDQTdMZTV5SDZCbHhPdWY1elBXcTNBNTd1cWRjSER0TT0=&h=279d4c37c7764be8a8cd92a53a018013&s=AVNPUEhUT0NFTkNSWVBUSVZpNWvhfkvsy0HBXeMJqMJXLVUzTeOGssiQ7jAnNTZ6aDtn6HKp77NuWaELZg1b57k From kate.bummer at seqmatic.com Thu Jun 22 10:38:20 2023 From: kate.bummer at seqmatic.com (Kate Bummer) Date: Thu, 22 Jun 2023 15:38:20 +0000 Subject: [Histonet] (no subject) Message-ID: Help on how to program an artifact, aka, Miles Tissue Tek VIP 1000 tissue processor? Hello All, My company purchased the miles tissue tek vip 1000 tissue processor at an auction and I have a user manual that is not very useful in figuring out how to set up programs. I was hoping to set up multiple programs but it looks like on the automatic it really only takes 1 program and you can set up start and end time but cannot set up multiple programs and would have to key in a new program every time. That is ok and not my preferred situation as I've really only worked with a Leica ASP300S which of course is much newer and can store several programs. Hoping someone has worked on one of these and may have a user guide that is more useful than the user manual I currently have (from 1986 : / ) Please drop me a line if you have any info it would be much appreciated. From relia1 at earthlink.net Thu Jun 22 10:49:02 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 22 Jun 2023 11:49:02 -0400 Subject: [Histonet] ICYMI Why leaving might be your best option for career advancement and some exciting job opportunities Message-ID: <006401d9a521$1254c730$36fe5590$@earthlink.net> Hi Histonet Histopeeps! I hope you are having a fantastic day! If you are someone who wants to move up in your career NOW is the perfect time to shine! Why? **The staffing shortages open opportunities for YOU to do MORE, Get MORE experience and further your career there or somewhere else. **I can speak from experience that my clients are giving motivated histotechs opportunities to be hired in at higher level positions based on potential not just immediate need! (That panic hiring is for travelers) I was surfing the internet and came across this article and had to share it with you: Why Leaving may be the best option for career advancement. Why Leaving Might Be the Best Option for Advancing Your Career [AOTJ] | Ivy Exec I am starting to get a lot of exciting opportunities and I wanted to touch base and let you know. * Your next opportunity might be just around the corner. * If you are looking for a position right now, please contact me right away. * We can talk about my current positions OR about a customized search on your behalf. * If you aren?t looking right away but want to let me know what would make a perfect job for you so that I could keep an eye out, that would be great too. * To do that, send me an email at relia1 at earthlink.net or call me on my cell at 407-353-5070. I have opportunities for advancement for: * Histology Managers * AP Managers * Lead Techs * Tech Support Specialists * Mohs Techs I have opportunities nationwide with some of the hottest areas being: * Florida * California * Arizona * Tennessee * Wisconsin * Arizona I also have some great opportunities for histotechs in: * California * Washington * Tennessee * South Carolina * Florida And new opportunities coming in on a daily basis! Most of these are RELIA Exclusives! That?s right you will only see them HERE! I really appreciate you taking time out of your busy day to read my e-mail Thanks Again!? Incidentally, if you have another area in mind let me know so I can be on the lookout. Thanks-Pam ? I would like to invite you to join my group on Facebook: www.facebook.com/groups/histotechnologists ? Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From bianca at alliedsearchpartners.com Thu Jun 22 13:26:00 2023 From: bianca at alliedsearchpartners.com ('Bianca Shalagin') Date: Thu, 22 Jun 2023 18:26:00 +0000 Subject: [Histonet] Histotech USA June, 2023 Opportunity Alert! Message-ID: <20230622182600.3305bf842131a304@alliedsearchpartners.com> li {display:list-item;text-indent: -1em;} ul, ol{margin-left: 40px !important; padding-left: 0px !important;} Multiple Histotechnician / Histotechnologist and Management positions are available in different states! These companies have some pretty good benefits and services worth looking into! Our company also offers a referral program as well should you know of anyone interested in advancing their career path. US Virgin Islands Syracuse, NY Boston, MA Billings, MT Plainview, NY Tampa, FL Area/Sarasota, FL area Jacksonville, FL Area Savannah, GA Area North of Los Angeles, CA Area Charleston, SC New York, NY (Just East of Manhattan) Benefits: ? Health, Dental, Vision ? Health Savings Account (HSA) ? Prescription RX Plan ? Basic Life & AD&D ? Voluntary Term Life ? Short Term Disability ? Critical Illness/Accident ? Hospital Indemnity ? Commuter Benefits/Housing/Relocation to Assignment ? Hearing Insurance Coverages ? Discounts on Travel, Auto Insurance, Electronics, Apparel, Education, Entertainment and much more! Opportunity Alert Multiple Histotech Positions in Different States! *If you do NOT see a position on our list that you are immediately interested in, please click ?INTERESTED? so we can talk with you about what your motivations are in your next career move! Allied Search Partners is currently conducting a search for: (Permanent/Temp or Temp-Perm) Histotech ? Billings, MT * Shift: M-F Day shift 8:30 am-5:00 pm * Competitive Pay + Relocation and Lodging Provided/Stipend for Housing * Must be ASCP eligible with an associate degree * Pay is $30-$35 with relocation and/or housing/stipend (Permanent) Histotech ? US Virgin Islands * Permanent position * Shift: M-F Day shift 8:30 am-5:00 pm * Competitive Pay + Relocation * Must be ASCP eligible with an associate degree (Permanent) Histotech ? near Jacksonville FL * Permanent position * Shift: M-F Day shift 8:30 am-5:00 pm * Pay: $25.48/hr.-$35.54/hr. + $3k in-state relocation / $10k out-of-state relocation * Must have Assoc. or a bachelor?s degree in chemical, physical or biological sciences. * Must have ASCP certification or if a new graduate, must have a test date scheduled. (Permanent) Histotech - near Sarasota, FL * Schedule: 2nd shift (2:00 pm-10:30 pm) * 3rd shift (10:30 pm-7:00 am) * Pay Competitive * Requirements: FL DOH Clinical Laboratory Personnel (Histology) license (Permanent) Histotech near Charleston, SC AREA * Must have or be eligible for HT, HTL (ASCP) * Pay: $30- $34/hr with a $2 shift diff on the 2nd shift + $2 if QIHC certified * Shift: Mon-Friday 2:30-11 pm * NO WEEKENDS! * Must be able to Embed and Cut (Permanent) Histotech outside of Savannah, GA AREA * Must have or be eligible for HT, HTL (ASCP) * Pay: $30- $34/hr with a $2 shift diff on the 2nd shift + $2 if QIHC certified * Shift: Mon-Friday 2:30-11 pm * NO WEEKENDS! * Must be able to Embed and Cut (Permanent) Histotech near Boston, MA AREA * ASCP Preferred NOT REQUIRED * Associate degree required * Pay: Competitive * Shifts: ANY/ ALL SHIFTS Available * Must be able to Embed and Cut (Temp/Permanent) MOHS Histotech Near NE Atlanta, GA AREA * NO ASCP Required * Must have at least 1 year (of MOHS experience RECENTLY) * Pay: $30-$35/hr. + Lodging * Shift: 4days/week (4/10s) * Must be able to Embed and Cut (Permanent) Histotech Supervisor near Bronx, NY AREA * Bachelor?s Degree required * Must have 6 years of Bench experience (master's degree, 2 years could be substituted) * Shift: Mon-Friday 9 am-5:30 pm * Pay: Around $120k * Must have ASCP * Must have NY Clinical License (Permanent) Histology Manager - East of Manhattan, NY * Bachelor?s Degree required * Two years of supervisory experience required * Shift: Day Shift Monday-Friday- Has Flexibility * Pay: $100-$120K with a 20% bonus * NY State License (Not Required) (Permanent) Histotech Technician - Long Island City, NY * Bachelor?s Degree required * Certification as a Histotechnician (HT) or Histotechnologist (HTL) is desirable but not required * Shift: Day Shift * Pay: Competitive * Experience in a preclinical research setting preferred (Permanent) Histotech near Bronx, NY AREA * Bachelor's degree Required * At Least 2 years of work experience * NY State license required and at least 2 years of grossing experience * Pay: $42.55-$52.96 * Shift: Mon-Friday 3 pm-11:30 pm * Must have experience with Rotary microtome, LIS (Laboratory Information System), Ph meter, Embedding, Cutting, LISA, Etc. (Permanent) Histotech Manager near North of Los Angeles, CA AREA * Must have 5 years of Lab and Histotech Experience * Must have at least 3 years of Management experience * ASCP preferred not required * Pay: up to $75k-$100k * Shift: Mon-Friday 6 am-3:30 pm Job Responsibilities The position is responsible for processing, embedding, cutting, and staining of tissue slides, accurate quality control, special projects, equipment maintenance, and all other duties assigned by the Team Lead or Laboratory Manager. Additional responsibilities of Histotech include. The jobs listed above may or may not include all or some of these duties. * Performs both routine and complex special Proce routine and complex special procedures with understanding duress with the understanding of tissue structures, techniques, principles, theory, and instrumentation. * May gross section and dictate descriptions of simple specimens, process, embed, cut, and stain all types of tissue specimens received in the Histology Department. * Coverslip-stained sections verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. * Assures specimen labeling requirements are met and necessary clinical information is available as needed. * Assists laboratory assistant or performs accessioning of patient specimens into the laboratory computer system when needed. * Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. * Selects and maintains appropriate tissue blocks to serve as controls for Special Stains. * Maintains all documentation that ensures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains, and IHC. * Able to process and decal bone specimens. * Able to prioritize the processing of specimens. * Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. * Monitor quality control results and take immediate and proper action when controls are unacceptable. * Follow defined procedures with only Lab Manager or pathologist-approved modifications or deviations. * Complete all instrument function verification, maintenance, and documents according to the procedure in the Histology area. Ensure that equipment defects and malfunctions are reported and repaired. * Maintains a neat, clean, and orderly work area. At the end of the day?s work; is responsible for the cleaning of his/her microtome. * Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. * May Cuts slides for IHC testing or other send-outs in conformance with procedures and submits them for analysis. * Must be able to express himself/herself effectively both in written and verbal communications. * Assists supervisory staff in monitoring workflow and ensuring that work/ staining priorities are met for the department. * Will be asked to put new tests/ stains online. * Participates in weekend and holiday schedules as staffing requirements dictate. * Remains flexible and works a share of overtime or different shifts if necessary, during staffing shortages or emergencies. * Participates in continuing education classes and courses. Are strongly encouraged to keep updated on recent advances in histology and to take at least 10 credit hours of continuing education a year. * Highly encouraged to maintain membership in a professional Histology organization. * Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. Successful candidates for the Histotech role include: HT, HTL(ASCP) registry or eligible (not required) Send a CV to: Bianca at alliedsearchpartners.com Contact Information: M-F 8:30 am - 5:00 pm (EST) (386)204-7886 https://calendly.com/bianca0 ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Fcalendly.com%2Fbianca09%2F30min ) 9 Bianca at alliedsearchpartners.com ( Bianca at alliedsearchpartners.com ) https://www.linkedin.com/in/bianca-shalagin619/ ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Fwww.linkedin.com%2Fin%2Fbianca-shalagin619%2F ) Connecting the Right Talent to the Right Organization! ASCP website: https://www.ascp.org/content/about-ascp/contact-ascp# ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Fwww.ascp.org%2Fcontent%2Fabout-ascp%2Fcontact-ascp%23 ) NY license application website: https://www.op.nysed.gov/professions/clinical-laboratory-technologists/license-options ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Fwww.op.nysed.gov%2Fprofessions%2Fclinical-laboratory-technologists%2Flicense-options ) FL license application website: https://floridasclinicallabs.gov/licensing/ ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Ffloridasclinicallabs.gov%2Flicensing%2F ) To schedule a call and make finding time easy please use my calendar link: https://calendly.com/bianca09 ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=https%3A%2F%2Fcalendly.com%2Fbianca09%2F30min ) Mrs. Bianca J Shalagin Allied Search Partners AN MRINETWORK MEMBER www.alliedsearchpartners.com ( https://app.loxo.co/agencies/13989/email_tracking/click?id=107893130&url=http%3A%2F%2Fwww.alliedsearchpartners.com%2F ) Bianca at alliedsearchpartners.com ? From jaylundgren at gmail.com Thu Jun 22 20:20:46 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 22 Jun 2023 18:20:46 -0700 Subject: [Histonet] (no subject) In-Reply-To: References: Message-ID: IIRC, yes, I seem to remember at AFIP our NCOIC cursing and reprogramming Roberta every Friday, or for a three day weekend. Roberta was the VIP's name. That lovely science-fictiony tan and burnt orange color scheme. I wonder what happened to her? Maybe you bought her? They never would have let a lowly Airman touch her. She would call you on the phone though, if her process was interrupted. No kidding, look around and you should find a land-line (of course) phone jack on the back. Pretty cool stuff in 1987. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Jun 22, 2023 at 8:42?AM Kate Bummer via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Help on how to program an artifact, aka, Miles Tissue Tek VIP 1000 tissue > processor? > > Hello All, > > My company purchased the miles tissue tek vip 1000 tissue processor at an > auction and I have a user manual that is not very useful in figuring out > how to set up programs. I was hoping to set up multiple programs but it > looks like on the automatic it really only takes 1 program and you can set > up start and end time but cannot set up multiple programs and would have to > key in a new program every time. That is ok and not my preferred situation > as I've really only worked with a Leica ASP300S which of course is much > newer and can store several programs. > > Hoping someone has worked on one of these and may have a user guide that > is more useful than the user manual I currently have (from 1986 : / ) > > Please drop me a line if you have any info it would be much appreciated. > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kendra.beechie at commonspirit.org Fri Jun 23 10:46:49 2023 From: kendra.beechie at commonspirit.org (Kendra Beechie ND-Bismarck) Date: Fri, 23 Jun 2023 10:46:49 -0500 Subject: [Histonet] ISH decal specimens Message-ID: Hello all, Recently, the Kappa/Lambda ISH has quit working on our decal specimens. When we first validated ISH, we tested decal specimens that stained appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial ISH validation back in 2019, but as far as I know, we have not changed which Decal solution we use since then. Has anyone else experienced this problem? I realize the other option is to run K/L IHC on the decal specimens, but we discontinued those after we brought the ISH on board. My medical director wants me to try to isolate the problem. My troubleshooting has only revealed that the decal is the common denominator. Thanks for any help! Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. From vtolley25 at gmail.com Fri Jun 23 11:08:25 2023 From: vtolley25 at gmail.com (Val Tolley) Date: Fri, 23 Jun 2023 09:08:25 -0700 Subject: [Histonet] ISH decal specimens In-Reply-To: References: Message-ID: Hi Kendra- Decal Stat from Statlab is HCl based, therefore is not appropriate for subsequent IHC, ISH, or other molecular studies. There is lots of peer-reviewed published data, if you need to supply rationale to your pathologists. HCl is known to significantly reduce molecular assay sensitivity and cause false negatives. Switch to either a formic-acid based decal (such as Immunocal) or EDTA-based decal and re-optimize your assay(s). Val Tolley, HTL (ASCP), QIHC > On Jun 23, 2023, at 8:53 AM, Kendra Beechie ND-Bismarck via Histonet wrote: > > ?Hello all, > > Recently, the Kappa/Lambda ISH has quit working on our decal specimens. > When we first validated ISH, we tested decal specimens that stained > appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial > ISH validation back in 2019, but as far as I know, we have not changed > which Decal solution we use since then. > > Has anyone else experienced this problem? I realize the other option is to > run K/L IHC on the decal specimens, but we discontinued those after we > brought the ISH on board. My medical director wants me to try to isolate > the problem. My troubleshooting has only revealed that the decal is the > common denominator. > > Thanks for any help! > > Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amyleehisto779 at gmail.com Fri Jun 23 12:39:43 2023 From: amyleehisto779 at gmail.com (Amy Lee) Date: Fri, 23 Jun 2023 10:39:43 -0700 Subject: [Histonet] IHC CRO Message-ID: Hello, Does anyone has a list of category can share that is used for IHC CRO comparison? I want to make a table to do comparison after talking to multiple CROs and I don't want to miss something. Just wondering if anyone did this before and can share your table or list? Thanks, Wendy From c.tague at pathologyarts.com Fri Jun 23 16:26:40 2023 From: c.tague at pathologyarts.com (Curt Tague) Date: Fri, 23 Jun 2023 21:26:40 +0000 Subject: [Histonet] distilled water systems Message-ID: Not sure what everyone else is doing but we're buying a TON of DI water for bulk IHC reagents... I'm looking at a system to install to produce our own. Anyone have any recommendations of a good, cost effective and efficient system? Thanks! Curt From greg.dobbin at gmail.com Sun Jun 25 10:26:21 2023 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Sun, 25 Jun 2023 12:26:21 -0300 Subject: [Histonet] ISH Decal specimens Message-ID: Use a formic acid based decal solution and MAKE SURE the specimen does not fix (formalin fixation) longer than 32hrs (false negatives result). Use 70% ETOH to manage the fixation time (ie fix for 24 hours, decal for 4 hrs, place in 70% ETOH until it gets put on the processor. Hold in 70% ETOH for weekends and holidays). Greg -- If God made a drink better than whiskey he kept it for himself! ? From dorianne.m.bonello at gov.mt Mon Jun 26 03:35:18 2023 From: dorianne.m.bonello at gov.mt (Bonello Dorianne M at Health-MDH) Date: Mon, 26 Jun 2023 08:35:18 +0000 Subject: [Histonet] Histology specimens marked as positive for Mycobacterium tuberculosis Message-ID: Dear all, How do you tackle specimens which are marked positive for Mycobacterium tuberculosis, fresh or fixed, since grossing does not involve the release of aerosols? Not sure if fixation with formalin actually deactivates the infectious agent. Read somewhere that there is an alcohol/formalin solution for such specimens prior to arriving at the lab. Regards, Dorianne Bonello Allied Health Practitioner (MLS) Histology Laboratory - Pathology Health-Mater Dei Hospital [cid:image001.jpg at 01D67184.63288530] T +356 +356 25456434 E dorianne.m.bonello at gov.mt Mater Dei Hospital, Triq id-Donaturi tad-Demm, l-Imsida, Malta MSD 2090 | Tel +356 2545 0000 | https://deputyprimeminister.gov.mt/en/MDH/Pages/Home.aspx | https://www.facebook.com/materdeihospital/ Think before you print. Kindly consider your environmental responsibility. This email and any files transmitted with it are confidential, may be legally privileged and intended solely for the use of the individual or entity to whom they are addressed. From SThompson4 at sonichealthcareusa.com Mon Jun 26 09:39:14 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Mon, 26 Jun 2023 14:39:14 +0000 Subject: [Histonet] Histology Jobs Message-ID: <2dfd9a7f963c4591a4ab25d530f68245@sonichealthcareusa.com> Sonic Healthcare USA has a Histology Supervisor role available in Exeter, New Hampshire. We also have histology positions available. This is a great lab, very family oriented, they love to help their employees learn and grow. Exeter is a beautiful area, if you are looking for your next adventure this is a great place to start. Please call me or email me your resume. sthompson4 at sonichealthcareusa.com This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From bianca at alliedsearchpartners.com Mon Jun 26 11:41:11 2023 From: bianca at alliedsearchpartners.com ('Bianca Shalagin') Date: Mon, 26 Jun 2023 16:41:11 +0000 Subject: [Histonet] Histotech, Histology Supervisor Job Opportunity nationwide! Message-ID: <20230626164111.a3e588f52868fbb2@alliedsearchpartners.com> li {display:list-item;text-indent: -1em;} ul, ol{margin-left: 40px !important; padding-left: 0px !important;} Hello {{first name}}, I am sorry to have missed you before, but if you do decide that this job opportunity is something you are interested in please reach out! I do believe that you would be a great fit for this opportunity based on your background and would love to connect with you on the position. Or if there is anyone else you would like to recommend for the position, I would love to hear your thoughts. Please see the position description below and let's talk! Bianca Opportunity Alert Multiple Histotech Positions in Different States! Allied Search Partners is currently conducting a search for: (Permanent/Temp or Temp-Perm) Histotech ? Billings, MT (Start ASAP) * Shift: M-F Day shift 8:30 am-5:00 pm * Competitive Pay + Relocation and Lodging Provided/Stipend for Housing * Must be ASCP eligible with an associate degree * Pay is $30-$35 with relocation and/or housing/stipend (Permanent) Histotech ? US Virgin Islands * Permanent position * Shift: M-F Day shift 8:30 am-5:00 pm * Competitive Pay + Relocation * Must be ASCP eligible with an associate degree (Permanent) Histotech ? near Jacksonville FL * Permanent position * Shift: M-F Day shift 8:30 am-5:00 pm * Pay: $25.48/hr.-$35.54/hr. + $3k in-state relocation / $10k out-of-state relocation * Must have Assoc. or a bachelor?s degree in chemical, physical or biological sciences. * Must have ASCP certification or if a new graduate, must have a test date scheduled. (Permanent) Histotech - near Sarasota, FL * Schedule: 2nd shift (2:00 pm-10:30 pm) * 3rd shift (10:30 pm-7:00 am) * Pay Competitive * Requirements: FL DOH Clinical Laboratory Personnel (Histology) license (Permanent) Histotech near Charleston, SC AREA * Must have or be eligible for HT, HTL (ASCP) * Pay: $30- $34/hr with a $2 shift diff on the 2nd shift + $2 if QIHC certified * Shift: Mon-Friday 2:30-11 pm * NO WEEKENDS! * Must be able to Embed and Cut (Permanent) Histotech outside of Savannah, GA AREA * Must have or be eligible for HT, HTL (ASCP) * Pay: $30- $34/hr with a $2 shift diff on the 2nd shift + $2 if QIHC certified * Shift: Mon-Friday 2:30-11 pm * NO WEEKENDS! * Must be able to Embed and Cut (Permanent) Histotech near Boston, MA AREA * ASCP Preferred NOT REQUIRED * Associate degree required * Pay: Competitive * Shifts: ANY/ ALL SHIFTS Available * Must be able to Embed and Cut (Temp/Permanent) MOHS Histotech Near NE Atlanta, GA AREA * NO ASCP Required * Must have at least 1 year (of MOHS experience RECENTLY) * Pay: $30-$35/hr. + Lodging * Shift: 4days/week (4/10s) * Must be able to Embed and Cut (Permanent) Histotech Supervisor near Bronx, NY AREA * Bachelor?s Degree required * Must have 6 years of Bench experience (master's degree, 2 years could be substituted) * Shift: Mon-Friday 9 am-5:30 pm * Pay: Around $120k * Must have ASCP * Must have NY Clinical License (Permanent) Histology Manager - East of Manhattan, NY * Bachelor?s Degree required * Two years of supervisory experience required * Shift: Day Shift Monday-Friday- Has Flexibility * Pay: $100-$120K with a 20% bonus * NY State License (Not Required) (Permanent) Histotech Technician - Long Island City, NY * Bachelor?s Degree required * Certification as a Histotechnician (HT) or Histotechnologist (HTL) is desirable but not required * Shift: Day Shift * Pay: Competitive * Experience in a preclinical research setting preferred (Permanent) Histotech near Bronx, NY AREA * Bachelor's degree Required * At Least 2 years of work experience * NY State license required and at least 2 years of grossing experience * Pay: $42.55-$52.96 * Shift: Mon-Friday 3 pm-11:30 pm * Must have experience with Rotary microtome, LIS (Laboratory Information System), Ph meter, Embedding, Cutting, LISA, Etc. (Permanent) Histotech Manager near North of Los Angeles, CA AREA * Must have 5 years of Lab and Histotech Experience * Must have at least 3 years of Management experience * ASCP preferred not required * Pay: up to $75k-$100k * Shift: Mon-Friday 6 am-3:30 pm US Virgin Islands Syracuse, NY Boston, MA Billings, MT Plainview, NY Tampa, FL Area/Sarasota, FL area Jacksonville, FL Area Savannah, GA Area North of Los Angeles, CA Area Charleston, SC New York, NY (Just East of Manhattan) Benefits: ? Health, Dental, Vision ? Health Savings Account (HSA) ? Prescription RX Plan ? Basic Life & AD&D ? Voluntary Term Life ? Short Term Disability ? Critical Illness/Accident ? Hospital Indemnity ? Commuter Benefits/Housing/Relocation to Assignment ? Hearing Insurance Coverages ? Discounts on Travel, Auto Insurance, Electronics, Apparel, Education, Entertainment and much more! Job Responsibilities The position is responsible for processing, embedding, cutting, and staining of tissue slides, accurate quality control, special projects, equipment maintenance, and all other duties assigned by the Team Lead or Laboratory Manager. Additional responsibilities of Histotech include. The jobs listed above may or may not include all or some of these duties. * Performs both routine and complex special Proce routine and complex special procedures with understanding duress with the understanding of tissue structures, techniques, principles, theory, and instrumentation. * May gross section and dictate descriptions of simple specimens, process, embed, cut, and stain all types of tissue specimens received in the Histology Department. * Coverslip-stained sections verify that the staining shows adequate cellular distinction, label, and distribute to the pathologist responsible for reviewing the case. * Assures specimen labeling requirements are met and necessary clinical information is available as needed. * Assists laboratory assistant or performs accessioning of patient specimens into the laboratory computer system when needed. * Demonstrates general knowledge of pathological and physiological conditions that affect test results and tissue staining. * Selects and maintains appropriate tissue blocks to serve as controls for Special Stains. * Maintains all documentation that ensures that all Special Stain control blocks have been adequately tested. Cuts and labels control slides for Histology, Special Stains, and IHC. * Able to process and decal bone specimens. * Able to prioritize the processing of specimens. * Recognize and troubleshoot both routine and complex problems and assist other Histotechs and lab assistants with technical problems. * Monitor quality control results and take immediate and proper action when controls are unacceptable. * Follow defined procedures with only Lab Manager or pathologist-approved modifications or deviations. * Complete all instrument function verification, maintenance, and documents according to the procedure in the Histology area. Ensure that equipment defects and malfunctions are reported and repaired. * Maintains a neat, clean, and orderly work area. At the end of the day?s work; is responsible for the cleaning of his/her microtome. * Responsible for the proper handling and disposal of all biohazardous materials and chemically hazardous materials including the neutralization or recycling of chemicals before disposal or reuse. * May Cuts slides for IHC testing or other send-outs in conformance with procedures and submits them for analysis. * Must be able to express himself/herself effectively both in written and verbal communications. * Assists supervisory staff in monitoring workflow and ensuring that work/ staining priorities are met for the department. * Will be asked to put new tests/ stains online. * Participates in weekend and holiday schedules as staffing requirements dictate. * Remains flexible and works a share of overtime or different shifts if necessary, during staffing shortages or emergencies. * Participates in continuing education classes and courses. Are strongly encouraged to keep updated on recent advances in histology and to take at least 10 credit hours of continuing education a year. * Highly encouraged to maintain membership in a professional Histology organization. * Always maintains a safe work environment and attends all safety training classes and conforms to all company safety guidelines and requirements. Successful candidates for the Histotech role include: HT, HTL(ASCP) registry or eligible (not required) Send a CV to: Bianca at alliedsearchpartners.com To schedule a call and make finding time easy please use my calendar link: https://calendly.com/bianca0 ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=https%3A%2F%2Fcalendly.com%2Fbianca09%2F30min ) 9 Connecting the Right Talent to the Right Organization! ASCP website: https://www.ascp.org/content/about-ascp/contact-ascp# ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=https%3A%2F%2Fwww.ascp.org%2Fcontent%2Fabout-ascp%2Fcontact-ascp%23 ) NY license application website: https://www.op.nysed.gov/professions/clinical-laboratory-technologists/license-options ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=https%3A%2F%2Fwww.op.nysed.gov%2Fprofessions%2Fclinical-laboratory-technologists%2Flicense-options ) FL license application website: https://floridasclinicallabs.gov/licensing/ ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=https%3A%2F%2Ffloridasclinicallabs.gov%2Flicensing%2F ) To schedule a call and make finding time easy please use my calendar link: https://calendly.com/bianca09 ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=https%3A%2F%2Fcalendly.com%2Fbianca09%2F30min ) Mrs. Bianca J Shalagin Allied Search Partners AN MRINETWORK MEMBER www.alliedsearchpartners.com ( https://app.loxo.co/agencies/13989/email_tracking/click?id=108245435&url=http%3A%2F%2Fwww.alliedsearchpartners.com%2F ) Bianca at alliedsearchpartners.com ? From relia1 at earthlink.net Wed Jun 28 11:57:27 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 28 Jun 2023 12:57:27 -0400 Subject: [Histonet] Just in time for July 4th I asked ChatGPT to create Fireworks using histological stains. Please enjoy and feel free to share! Message-ID: <00a101d9a9e1$9f9aac30$ded00490$@earthlink.net> Hi Histopeeps I hope you are having a fantastic day. Can you believe it?s 4th of July next week? If you have been reading my emails then you know I have been having some fun using ChatGPT to create images. In honor of Independence Day Here is a link to an image of Fireworks created by ChatGPT using histological stains: https://reliasolutionspambarker.wordpress.com/2023/06/28/just-in-time-for-ju ly-4th-i-asked-chatgpt-to-create-fireworks-using-histological-stains-please- feel-free-to-enjoy-and-share/(opens in a new tab) Histopeeps, What do you think? It?s a holiday week so I will keep the job info short and sweet. Take a look and see if you or anyone you know might be interested in any of these opportunities. * If something looks interesting let me know. * If you know someone who might be interested, please pass along the information. Remember if I place someone you refer to me you will earn a referral fee. If the opportunity for you is someplace else drop me an email to remind me where you want to be and what you want to do. I want YOU to be the person that pops in MY head when that client calls!!! Here are my Hottest!! Histology Opportunities: RELIA Histology Opportunities: I am recruiting for these positions: * AP Manager * Quality Assurance Specialist * Tech Support Specialist * Histology Manager * Lead Histotech * Histotechnicians/Histotechnologists * Pathologist?s Assistant Locations are nationwide including: Florida, California, Tennessee, Georgia, Wisconsin, New York, Arizona, Washington & South Carolina. I have brand new labs, hospitals, derm, gi, research private labs and new start ups. All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign-on bonuses. All of these jobs are full-time & permanent & some are RELIA Exclusives!!! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. If you want to chat ASAP call or text me at 407-353-5070 and if you want to set up a time to chat call the office at 866-607-3542 or email me at relia1 at earthlink.net with a time and the best number to call you!!!! I can?t wait to chat with you!! I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ?Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ? ? ? ? From Pat.Patterson at propath.com Wed Jun 28 21:43:13 2023 From: Pat.Patterson at propath.com (Pat Patterson) Date: Thu, 29 Jun 2023 02:43:13 +0000 Subject: [Histonet] Histonet Digest, Vol 235, Issue 23 In-Reply-To: References: Message-ID: Get Outlook for Android ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Wednesday, June 28, 2023 12:00:02 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 235, Issue 23 [CAUTION - EXTERNAL EMAIL: Do not click any links or open any attachments unless you trust the sender and know the content is safe.] Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=B6hplVa%2BckxfPcyrNpk2XeuPNcE%2FMIdt8LTGcQJYnMY%3D&reserved=0 or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Just in time for July 4th I asked ChatGPT to create Fireworks using histological stains. Please enjoy and feel free to share! (relia1 at earthlink.net) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 Jun 2023 12:57:27 -0400 From: To: Subject: [Histonet] Just in time for July 4th I asked ChatGPT to create Fireworks using histological stains. Please enjoy and feel free to share! Message-ID: <00a101d9a9e1$9f9aac30$ded00490$@earthlink.net> Content-Type: text/plain; charset="utf-8" Hi Histopeeps I hope you are having a fantastic day. Can you believe it?s 4th of July next week? If you have been reading my emails then you know I have been having some fun using ChatGPT to create images. In honor of Independence Day Here is a link to an image of Fireworks created by ChatGPT using histological stains: https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Freliasolutionspambarker.wordpress.com%2F2023%2F06%2F28%2Fjust-in-time-for-ju&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=n%2F2YonZJXXZ%2F0r6g8EBS3mwP8xCSJcDoCw9nd64VOdA%3D&reserved=0 ly-4th-i-asked-chatgpt-to-create-fireworks-using-histological-stains-please- feel-free-to-enjoy-and-share/(opens in a new tab) Histopeeps, What do you think? It?s a holiday week so I will keep the job info short and sweet. Take a look and see if you or anyone you know might be interested in any of these opportunities. * If something looks interesting let me know. * If you know someone who might be interested, please pass along the information. Remember if I place someone you refer to me you will earn a referral fee. If the opportunity for you is someplace else drop me an email to remind me where you want to be and what you want to do. I want YOU to be the person that pops in MY head when that client calls!!! Here are my Hottest!! Histology Opportunities: RELIA Histology Opportunities: I am recruiting for these positions: * AP Manager * Quality Assurance Specialist * Tech Support Specialist * Histology Manager * Lead Histotech * Histotechnicians/Histotechnologists * Pathologist?s Assistant Locations are nationwide including: Florida, California, Tennessee, Georgia, Wisconsin, New York, Arizona, Washington & South Carolina. I have brand new labs, hospitals, derm, gi, research private labs and new start ups. All of my clients offer excellent compensation, benefits and some offer relocation assistance and or sign-on bonuses. All of these jobs are full-time & permanent & some are RELIA Exclusives!!! If you or anyone you know is interested in hearing more about any of these opportunities please contact me. Remember if I place someone you refer to me you will earn a referral bonus. If you want to chat ASAP call or text me at 407-353-5070 and if you want to set up a time to chat call the office at 866-607-3542 or email me at relia1 at earthlink.net with a time and the best number to call you!!!! I can?t wait to chat with you!! I welcome you to join my group on Facebook: https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Ffacebook.com%2Fgroups%2Fhistotechnologists&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NfBTOAjNhVB6d1erJmBnFBGEoLCpZK7GNsmCWILdZD4%3D&reserved=0 Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! ?Thank You! ?Pam M. Barker? Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Fwww.facebook.com%2FRELIASolutionsforhistologyprofessionals&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=l%2FKRld6cYJzeXyklO90xy1KrwGwI5KdaL57UPhvwn%2Bc%3D&reserved=0 I welcome you to join my group on Facebook: https://nam12.safelinks.protection.outlook.com/?url=https%3A%2F%2Ffacebook.com%2Fgroups%2Fhistotechnologists&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NfBTOAjNhVB6d1erJmBnFBGEoLCpZK7GNsmCWILdZD4%3D&reserved=0 https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Fwww.linkedin.com%2Fin%2Freliasolutions&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=kF%2F3EBJgQHr0978bfyC%2FbVyLMTViV15xXwg32Mo6Bpg%3D&reserved=0 > Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology ? ? ? ? ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cpat.patterson%40propath.com%7C9b575aad84c44847c4cf08db77fb4ad7%7Ceab7e4b5d8f8463b8a4ac63f87390803%7C1%7C0%7C638235693405624313%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=B6hplVa%2BckxfPcyrNpk2XeuPNcE%2FMIdt8LTGcQJYnMY%3D&reserved=0 ------------------------------ End of Histonet Digest, Vol 235, Issue 23 ***************************************** From plucas at biopath.org Thu Jun 29 10:59:54 2023 From: plucas at biopath.org (Paula) Date: Thu, 29 Jun 2023 08:59:54 -0700 Subject: [Histonet] tissue in alcohol too long now brittle Message-ID: <005801d9aaa2$bfa5d380$3ef17a80$@biopath.org> Hello, This is a question for a colleague of mine. Her routine tissue samples were left in 95% alcohol for 2 hours before she had a chance to get to it. She finished the processing, but now the gi's are extremely brittle and have holes. She is afraid of reprocessing and doesn't know what to do. Can anyone who experienced this have a remedy to share? Thank you, Paula From jaylundgren at gmail.com Thu Jun 29 11:49:09 2023 From: jaylundgren at gmail.com (Jay Lundgren) Date: Thu, 29 Jun 2023 09:49:09 -0700 Subject: [Histonet] tissue in alcohol too long now brittle In-Reply-To: <005801d9aaa2$bfa5d380$3ef17a80$@biopath.org> References: <005801d9aaa2$bfa5d380$3ef17a80$@biopath.org> Message-ID: Soak in Oil of Cedar. Sincerely, A histotech from 1901 On Thu, Jun 29, 2023 at 9:00?AM Paula via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello, > > This is a question for a colleague of mine. Her routine tissue samples were > left in 95% alcohol for 2 hours before she had a chance to get to it. She > finished the processing, but now the gi's are extremely brittle and have > holes. She is afraid of reprocessing and doesn't know what to do. > > Can anyone who experienced this have a remedy to share? > > Thank you, > > Paula > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From AHorvath at centpath.com Fri Jun 30 13:41:02 2023 From: AHorvath at centpath.com (Andrew Horvath) Date: Fri, 30 Jun 2023 12:41:02 -0600 Subject: [Histonet] unprocessed tissue and Excelsior AS issue Message-ID: <006c01d9ab82$6b768d50$4263a7f0$@centpath.com> Hello all, I have two things to address in this email. First, I am trying to locate tissue that has not been processed so we can use it for testing of our tissue processors. Optimally it would be colon but also esophagus as we are a GI lab. Does anyone know where I can find such tissue (in the US preferably)? Second, we use the Epredia Excelsior AS processor and have run into issues with the processing of tissue where portions of small biopsy specimens will have sections that appear to not be processed. It does not happen on every specimen but is consistent enough we are trying to find solutions to this problem. Hence the non-processed tissue I am trying to locate. Any help is appreciated! Thanks, Andrew Andrew Horvath, MBA, MA Business Operations Manager 303-220-4369 Centennial Pathologists, PC 7346 South Alton Way, Suite 10-D Centennial, CO 80112 Confidentiality Notice: This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, or confidential. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication From eddessa at emory.edu Fri Jun 30 14:08:55 2023 From: eddessa at emory.edu (Dessasau, Evan D.) Date: Fri, 30 Jun 2023 19:08:55 +0000 Subject: [Histonet] [External] unprocessed tissue and Excelsior AS issue In-Reply-To: <006c01d9ab82$6b768d50$4263a7f0$@centpath.com> References: <006c01d9ab82$6b768d50$4263a7f0$@centpath.com> Message-ID: Hi Mr. Horvath, I work at a Biomedical research facility with non-human primates as the main lab animal. If you could use wet tissue from non-human primates, you could submit a Specimen Request for access to tissue for your project. If interested, please email Mrs. Summerville cc'ed here. E-van -----Original Message----- From: Andrew Horvath via Histonet Sent: Friday, June 30, 2023 2:41 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] unprocessed tissue and Excelsior AS issue Hello all, I have two things to address in this email. First, I am trying to locate tissue that has not been processed so we can use it for testing of our tissue processors. Optimally it would be colon but also esophagus as we are a GI lab. Does anyone know where I can find such tissue (in the US preferably)? Second, we use the Epredia Excelsior AS processor and have run into issues with the processing of tissue where portions of small biopsy specimens will have sections that appear to not be processed. It does not happen on every specimen but is consistent enough we are trying to find solutions to this problem. Hence the non-processed tissue I am trying to locate. Any help is appreciated! Thanks, Andrew Andrew Horvath, MBA, MA Business Operations Manager 303-220-4369 Centennial Pathologists, PC 7346 South Alton Way, Suite 10-D Centennial, CO 80112 Confidentiality Notice: This message (including any attachments) is intended only for the use of the individual or entity to which it is addressed and may contain information that is non-public, proprietary, privileged, or confidential. If you are not the intended recipient, you are hereby notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, notify us immediately by telephone and (i) destroy this message if a facsimile or (ii) delete this message immediately if this is an electronic communication _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From acanabal at ciencias.unam.mx Fri Jun 30 18:51:01 2023 From: acanabal at ciencias.unam.mx (=?UTF-8?Q?Alonso_Mart=C3=ADnez_Canabal?=) Date: Fri, 30 Jun 2023 17:51:01 -0600 Subject: [Histonet] [External] unprocessed tissue and Excelsior AS issue In-Reply-To: References: <006c01d9ab82$6b768d50$4263a7f0$@centpath.com> Message-ID: Good morning, I want to perform immunohistochemistry in mouse postnatal brains of 0,5,10 days. I have seen that many people, including Paxinos, make the sections including the skull. Do you think that I should follow this using a protocol for decalcification of the skull? Thank you! El vie, 30 jun 2023 a las 13:21, Dessasau, Evan D. via Histonet (< histonet at lists.utsouthwestern.edu>) escribi?: > Hi Mr. Horvath, I work at a Biomedical research facility with non-human > primates as the main lab animal. If you could use wet tissue from > non-human primates, you could submit a Specimen Request for access to > tissue for your project. If interested, please email Mrs. Summerville > cc'ed here. > E-van > > > > -----Original Message----- > From: Andrew Horvath via Histonet > Sent: Friday, June 30, 2023 2:41 PM > To: histonet at lists.utsouthwestern.edu > Subject: [External] [Histonet] unprocessed tissue and Excelsior AS issue > > Hello all, > > > > I have two things to address in this email. > > > > First, I am trying to locate tissue that has not been processed so we can > use it for testing of our tissue processors. Optimally it would be colon > but also esophagus as we are a GI lab. Does anyone know where I can find > such tissue (in the US preferably)? > > > > Second, we use the Epredia Excelsior AS processor and have run into issues > with the processing of tissue where portions of small biopsy specimens will > have sections that appear to not be processed. It does not happen on every > specimen but is consistent enough we are trying to find solutions to this > problem. Hence the non-processed tissue I am trying to locate. > > > > Any help is appreciated! > > > > > > > > Thanks, > > > > Andrew > > > > > > Andrew Horvath, MBA, MA > > Business Operations Manager > > 303-220-4369 > > > > Centennial Pathologists, PC > > 7346 South Alton Way, Suite 10-D > > Centennial, CO 80112 > > > > > > Confidentiality Notice: > > This message (including any attachments) is intended only for the use of > the individual or entity to which it is addressed and may contain > information that is non-public, proprietary, privileged, or confidential. > If you are not the intended recipient, you are hereby notified that any > use, dissemination, distribution, or copying of this communication is > strictly prohibited. If you have received this communication in error, > notify us immediately by telephone and (i) destroy this message if a > facsimile or (ii) delete this message immediately if this is an electronic > communication > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Dr. Alonso Mart?nez Canabal PhD Profesor Asociado "C" Departamento de Biolog?a Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833