From gu.lang at gmx.at Sat Jul 1 13:57:14 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Sat, 1 Jul 2023 20:57:14 +0200 Subject: [Histonet] shelf life of working antibody solutions in IHC Message-ID: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> Hi all! I have tried to find a general instruction for the shelf life of antibody working solutions. With automated IHC you usually fill the container with the working solution and depending on the frequency of usage they stay on the instrument at roomtemperature (or higher) or are put in the fridge again. The working solutions are up to 10 ml and may last for months. The antibody-diluent is from the same company of the instrument. The titers are in a range from 1:10 to 1:3000. - so a very heterogen situation. How do you handle this? Have you a general rule, when the solution has to be discarded? Is it just a matter of positiv-controls? Thanks in advance Gudrun Lang Biomedical scientist Austria From afhenwood at outlook.com Sat Jul 1 16:36:12 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Sat, 1 Jul 2023 21:36:12 +0000 Subject: [Histonet] shelf life of working antibody solutions in IHC In-Reply-To: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> References: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> Message-ID: Hi Gudrun, This should be useful ( I will send a copy separately) Henwood, A. F. (2023). Validation of nominally expired antibodies for immunohistochemistry. Biotechnic & Histochemistry, 98(2), 86-93. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Gudrun Lang via Histonet Sent: Sunday, 2 July 2023 4:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] shelf life of working antibody solutions in IHC Hi all! I have tried to find a general instruction for the shelf life of antibody working solutions. With automated IHC you usually fill the container with the working solution and depending on the frequency of usage they stay on the instrument at roomtemperature (or higher) or are put in the fridge again. The working solutions are up to 10 ml and may last for months. The antibody-diluent is from the same company of the instrument. The titers are in a range from 1:10 to 1:3000. - so a very heterogen situation. How do you handle this? Have you a general rule, when the solution has to be discarded? Is it just a matter of positiv-controls? Thanks in advance Gudrun Lang Biomedical scientist Austria _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nmargaryan88 at gmail.com Sat Jul 1 17:06:39 2023 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Sat, 1 Jul 2023 17:06:39 -0500 Subject: [Histonet] shelf life of working antibody solutions in IHC In-Reply-To: References: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> Message-ID: Hi Tony, Very interesting article you shared. Hi Gudrun, I will only add to this - run positive and negative tests for every run. Good luck! Naira On Sat, Jul 1, 2023 at 4:40 PM Tony Henwood via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hi Gudrun, > > This should be useful ( I will send a copy separately) > > Henwood, A. F. (2023). Validation of nominally expired antibodies for > immunohistochemistry. Biotechnic & Histochemistry, 98(2), 86-93. > > Regards, > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > Principal Scientist, the Children?s Hospital at Westmead (Retired) > Adjunct Fellow, School of Medicine, University of Western Sydney. > > From: Gudrun Lang via Histonet > Sent: Sunday, 2 July 2023 4:59 AM > To: histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu> > Subject: [Histonet] shelf life of working antibody solutions in IHC > > Hi all! > > I have tried to find a general instruction for the shelf life of antibody > working solutions. > > With automated IHC you usually fill the container with the working solution > and depending on the frequency of usage they stay on the instrument at > roomtemperature (or higher) or are put in the fridge again. > > The working solutions are up to 10 ml and may last for months. The > antibody-diluent is from the same company of the instrument. The titers are > in a range from 1:10 to 1:3000. - so a very heterogen situation. > > > > How do you handle this? Have you a general rule, when the solution has to > be > discarded? Is it just a matter of positiv-controls? > > > > Thanks in advance > > Gudrun Lang > > Biomedical scientist > > Austria > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From afhenwood at outlook.com Sun Jul 2 19:07:50 2023 From: afhenwood at outlook.com (Tony Henwood) Date: Mon, 3 Jul 2023 00:07:50 +0000 Subject: [Histonet] shelf life of working antibody solutions in IHC In-Reply-To: <001501d9acc7$3a46fb20$aed4f160$@gmx.at> References: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> <001501d9acc7$3a46fb20$aed4f160$@gmx.at> Message-ID: In Australia, we have evolved from Prescriptive accreditation (following the standards) to Risk-based accreditation where it is more important to assess the risk of a laboratory process rather than only meeting a list of rules, since following a list of rules is often not in the patient?s nor in the community?s interest. Years ago, I battled with our accreditation organization over the risk involved in fridge temperature recording, presenting evidence that continuous digital monitoring (with an alarm system) was less risky than recording max/min temperatures daily (eg what happens when the lab is not manned during Christmas holidays and weekends?). The excessive cost involved in automatically disposing of expired antibodies is wasteful. Continuous monitoring as well as preventative practices such as preservatives (azide, procillin, etc) and pH monitoring with phenol red greatly reduces the risk. As presented in the paper, many working dilutions survive without contamination for many years. For example, factor 8 (21 years), factor 13a (19 years) and epithelial membrane antigen (17 years). There were no failed verifications and to date, the average life after expiration was 6 years; eight antibodies exceeded 6 years. All the other antibodies would probably have similar survival times but were exhausted before reaching these times. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Gudrun Lang Sent: Sunday, 2 July 2023 7:26 PM To: 'Tony Henwood' Subject: AW: [Histonet] shelf life of working antibody solutions in IHC Dear Tony, thank you for the interesting article. It reflects my personal experience. Nevertheless accreditation rules overrule the experience and in an diagnostic lab we have to discard the reagenses. My question regards more the practical approach with working solutions. What is your experience how long the working solutions are ?good enough? for using. Thank you very much and kind regards Gudrun Von: Tony Henwood [mailto:afhenwood at outlook.com] Gesendet: Samstag, 1. Juli 2023 23:41 An: Gudrun Lang Betreff: RE: [Histonet] shelf life of working antibody solutions in IHC Here it is Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children?s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: Gudrun Lang via Histonet Sent: Sunday, 2 July 2023 4:59 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] shelf life of working antibody solutions in IHC Hi all! I have tried to find a general instruction for the shelf life of antibody working solutions. With automated IHC you usually fill the container with the working solution and depending on the frequency of usage they stay on the instrument at roomtemperature (or higher) or are put in the fridge again. The working solutions are up to 10 ml and may last for months. The antibody-diluent is from the same company of the instrument. The titers are in a range from 1:10 to 1:3000. - so a very heterogen situation. How do you handle this? Have you a general rule, when the solution has to be discarded? Is it just a matter of positiv-controls? Thanks in advance Gudrun Lang Biomedical scientist Austria _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cforster at umn.edu Mon Jul 3 12:47:50 2023 From: cforster at umn.edu (Colleen Forster) Date: Mon, 3 Jul 2023 12:47:50 -0500 Subject: [Histonet] shelf life of working antibody solutions in IHC In-Reply-To: References: <000001d9ac4d$d9f19dd0$8dd4d970$@gmx.at> <001501d9acc7$3a46fb20$aed4f160$@gmx.at> Message-ID: I run a research lab with automated staining and truthfully,I make my dilutions fresh for each run because proteins are so different in their stability. Colleen Forster HT(ASCP)QIHC On Sun, Jul 2, 2023 at 7:08?PM Tony Henwood via Histonet < histonet at lists.utsouthwestern.edu> wrote: > In Australia, we have evolved from Prescriptive accreditation (following > the standards) to Risk-based accreditation where it is more important to > assess the risk of a laboratory process rather than only meeting a list of > rules, since following a list of rules is often not in the patient?s nor in > the community?s interest. > > Years ago, I battled with our accreditation organization over the risk > involved in fridge temperature recording, presenting evidence that > continuous digital monitoring (with an alarm system) was less risky than > recording max/min temperatures daily (eg what happens when the lab is not > manned during Christmas holidays and weekends?). > > The excessive cost involved in automatically disposing of expired > antibodies is wasteful. Continuous monitoring as well as preventative > practices such as preservatives (azide, procillin, etc) and pH monitoring > with phenol red greatly reduces the risk. > > As presented in the paper, many working dilutions survive without > contamination for many years. For example, factor 8 (21 years), factor 13a > (19 years) and epithelial membrane antigen (17 years). There were no failed > verifications and to date, the average life after expiration was 6 years; > eight antibodies exceeded 6 years. All the other antibodies would probably > have similar survival times but were exhausted before reaching these times. > > Regards, > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > Principal Scientist, the Children?s Hospital at Westmead (Retired) > Adjunct Fellow, School of Medicine, University of Western Sydney. > > From: Gudrun Lang > Sent: Sunday, 2 July 2023 7:26 PM > To: 'Tony Henwood' > Subject: AW: [Histonet] shelf life of working antibody solutions in IHC > > Dear Tony, > thank you for the interesting article. It reflects my personal experience. > Nevertheless accreditation rules overrule the experience and in an > diagnostic lab we have to discard the reagenses. > > My question regards more the practical approach with working solutions. > What is your experience how long the working solutions are ?good enough? > for using. > > Thank you very much and kind regards > Gudrun > > Von: Tony Henwood [mailto:afhenwood at outlook.com] > Gesendet: Samstag, 1. Juli 2023 23:41 > An: Gudrun Lang > Betreff: RE: [Histonet] shelf life of working antibody solutions in IHC > > Here it is > > Regards, > > Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) > Principal Scientist, the Children?s Hospital at Westmead (Retired) > Adjunct Fellow, School of Medicine, University of Western Sydney. > > From: Gudrun Lang via Histonet > Sent: Sunday, 2 July 2023 4:59 AM > To: histonet at lists.utsouthwestern.edu histonet at lists.utsouthwestern.edu> > Subject: [Histonet] shelf life of working antibody solutions in IHC > > Hi all! > > I have tried to find a general instruction for the shelf life of antibody > working solutions. > > With automated IHC you usually fill the container with the working solution > and depending on the frequency of usage they stay on the instrument at > roomtemperature (or higher) or are put in the fridge again. > > The working solutions are up to 10 ml and may last for months. The > antibody-diluent is from the same company of the instrument. The titers are > in a range from 1:10 to 1:3000. - so a very heterogen situation. > > > > How do you handle this? Have you a general rule, when the solution has to > be > discarded? Is it just a matter of positiv-controls? > > > > Thanks in advance > > Gudrun Lang > > Biomedical scientist > > Austria > > > > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Colleen Forster HT(ASCP)QIHC BLS Histology and IHC Laboratory Jackson Hall, Room 2-155 321 Church St. SE Minneapolis, MN 55455 612-626-1930 From rmccormick10 at yahoo.com Mon Jul 3 13:21:41 2023 From: rmccormick10 at yahoo.com (Rhonda McCormick) Date: Mon, 3 Jul 2023 18:21:41 +0000 (UTC) Subject: [Histonet] (no subject) References: <1243461411.1454660.1688408501998.ref@mail.yahoo.com> Message-ID: <1243461411.1454660.1688408501998@mail.yahoo.com> Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram negative bacteria. If so, would you mind to send your protocol please? Any recommendations for a gram negative (only) stain - specifically E. coli? One of our residents is asking. Thank you! From jmacdonald at mtsac.edu Mon Jul 3 14:18:47 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Mon, 3 Jul 2023 19:18:47 +0000 Subject: [Histonet] (no subject) In-Reply-To: <1243461411.1454660.1688408501998@mail.yahoo.com> References: <1243461411.1454660.1688408501998.ref@mail.yahoo.com> <1243461411.1454660.1688408501998@mail.yahoo.com> Message-ID: We have good success with the Twort. -----Original Message----- From: Rhonda McCormick via Histonet Sent: Monday, July 3, 2023 11:22 AM To: Histonet Subject: [Histonet] (no subject) EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram negative bacteria. If so, would you mind to send your protocol please? Any recommendations for a gram negative (only) stain - specifically E. coli? One of our residents is asking. Thank you! _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgflem at gmail.com Tue Jul 4 15:12:55 2023 From: mgflem at gmail.com (Matthew Fleming) Date: Tue, 4 Jul 2023 15:12:55 -0500 Subject: [Histonet] Full-time histotech job in Milwaukee, WI Message-ID: I am posting the following for Affiliated Dermatologists. I am a dermatopathologist with my own lab, but work part-time as a dermatologist for Affiliated Dermatologists. It's a nice group; if you're looking for work in the area I doubt you can do better. The position is with Affiliated's Mohs surgery unit at their Oak Creek location. If interested, please send CV or equivalent and cover letter/message with availability to: Veronica Sanchez Director of Operations Affiliated Dermatologists S.C. 13800 West North Ave Suite 100 Brookfield, Wisconsin 53005 vsanchez at affiliatedderm.com *Affiliated Dermatologists is seeking a Mohs technician full time to help our growing practice.* Job description: Provides support to our Mohs surgeon. This includes accurately preparing and processing histologic specimens for microscopic evaluation and review by the Mohs surgeon. Maintains appropriate quality controls, and ensures the department is operating in accordance with all standards set forth by OSHA, CLIA and other regulatory agencies. Essential Functions: Processes, embeds, cuts and stains quality frozen tissue specimens. Performs and documents tests in compliance with quality control procedures and established parameters and standards. Maintains appropriate quality controls, and ensures the department is operating in accordance with all standards set forth by OSHA, CLIA and other regulatory agencies. Maintains histology equipment in good working order and troubleshoots equipment problems. Works with the Mohs surgeon to resolve quality control, patient, or procedural problems. Orders supplies related to the defined area. Cross trains appropriate staff on job duties. Consistently follows policies, procedures, and protocols at Affiliated Dermatology. Maintains an extremely high level of confidentiality and follows HIPAA guidelines. Responsible for delivering a high level of customer service in all in interactions with patients, providers, employees, vendors, and guests. Assists in meeting and exceeding individual, team and organizational goals. Performs other duties as assigned. Maintains yearly competencies. Job Type: Full-time Salary: Based on experience Benefits: - 401(k) - Dental insurance - Health insurance - Vision insurance - PTO Matthew Fleming, MD Fleming Dermatopathology and Affiliated Dermatologists Milwaukee, WI From Donna.Willis at BSWHealth.org Wed Jul 5 11:44:11 2023 From: Donna.Willis at BSWHealth.org (Willis, Donna G) Date: Wed, 5 Jul 2023 16:44:11 +0000 Subject: [Histonet] DreamPath Block and Slide Storage Systems Message-ID: Is there anyone on the Histonet that is currently using the DreamPath Block and Slide Storage System? Can you give me your opinion of the company and the product. Thanks, Donna Willis HT/HTL (ASCP) Anatomic Pathology Manager Baylor Scott&White Health Baylor University Medical Center 3500 Gaston Ave. Dallas, Texas 75246 214-820-2465 office 214-725-6184 cell ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From SThompson4 at sonichealthcareusa.com Wed Jul 5 16:29:52 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Wed, 5 Jul 2023 21:29:52 +0000 Subject: [Histonet] Histotechnologist Opportunities Message-ID: <30812523ae6f4863b7ba1d6124255189@sonichealthcareusa.com> Hello Everyone, Sonic Healthcare USA has several opportunities available for histotechnologists located in beautiful Exeter, New Hampshire. We also have a position in Ridgeland, South Carolina, rare opportunity, the turnover is very low at this lab. It is located very near Savannah, Georgia, a great place to visit with its quaint charm. Sonic Healthcare USA is a subsidiary of Sonic Healthcare Limited, one of the world's largest medical diagnostic companies, providing laboratory services to medical practitioners, hospitals and community health services, with operations in eight countries, on three continents and providing care to over 100 million patients each year. We offer full medical benefits, matching 401k, and more. Reach out to me directly at sthompson4 at sonichealthcareusa.com Or visit our website at www.sonichealthcareusa.com You won't regret it!! Stephanie Thompson 210-428-1646 This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From criley at udel.edu Thu Jul 6 10:30:26 2023 From: criley at udel.edu (Charles Riley) Date: Thu, 6 Jul 2023 11:30:26 -0400 Subject: [Histonet] Possible causes for tissue tears Message-ID: Hello all, I tried posting this question earlier but the file size was too big. I processed a piece of tissue engineered cartilage the other day for a research project and stained it with a few different stains. Each sample has various tears through the tissue (that don't appear to be microtomy artifacts in my opinion). I was hoping to get some feedback as to what everyone thinks may be the most likely cause. I have a image I can send directly to you if you reply for added info. From alapiana12 at gmail.com Fri Jul 7 08:19:38 2023 From: alapiana12 at gmail.com (Anthony Lapiana) Date: Fri, 7 Jul 2023 09:19:38 -0400 Subject: [Histonet] DreamPath Block and Slide Storage Systems Message-ID: Hi Donna, I currently use dreampath storage system ARCOS for both slide and block storage, we have an extremely high volume, and it handles the system almost flawlessly. The support team is based in France I believe but someone is always available for remote service. The only negative part I have experienced is memory issues with the computer, we have updated our computer twice in the last 3 years, other than that I highly recommend it if you?re looking for an automatic storage system. *Lapiana, Anthony, HT(ASCP)* Laboratory Supervisor, IHC/SPU Massachusetts General Hospital From tonyreilly55 at gmail.com Fri Jul 7 09:35:38 2023 From: tonyreilly55 at gmail.com (Tony Reilly) Date: Sat, 8 Jul 2023 00:35:38 +1000 Subject: [Histonet] (no subject) Message-ID: <75388696-1C32-4323-85B6-C0BBE9F65C2B@gmail.com> ?Hello Rhonda I have extensive experience with the Brown Hopps stain. I would advise against only staining for gram negative as both the gram positive stain and the gram negative stain will stain both gram positive and gram negative organisms. The differentiation is only achieved by the destaining steps. If you only used the gram negative steps you would still be staining both gram positive and gram negative organisms negatively. If you you want more information let me know . Regards Tony Reilly Histologist Australia Sent from my iPhone > On 4 Jul 2023, at 5:30 am, Mac Donald, Jennifer via Histonet wrote: > ?We have good success with the Twort. > > -----Original Message----- > From: Rhonda McCormick via Histonet > Sent: Monday, July 3, 2023 11:22 AM > To: Histonet > Subject: [Histonet] (no subject) > > EXTERNAL SENDER - Exercise caution with requests, links, and attachments. > > Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram negative bacteria. If so, would you mind to send your protocol please? > Any recommendations for a gram negative (only) stain - specifically E. coli? One of our residents is asking. > Thank you! > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica.Piche at wtbyhosp.org Tue Jul 11 13:01:02 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Tue, 11 Jul 2023 18:01:02 +0000 Subject: [Histonet] Tissue disoposal/formalin Message-ID: Good Afternoon, I have a question regarding the dumping of tissue. How are your laboratories dumping tissue when it is time for disposal? Are you putting the specimens with 10% neutral buffered formalin in the waste containers and a company comes and removes them from your facility? I would appreciate any information that you can share. Thank you, Jessica Piche Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From Charles.Bacon at baystatehealth.org Wed Jul 12 12:25:26 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Wed, 12 Jul 2023 17:25:26 +0000 Subject: [Histonet] Tissue disoposal/formalin In-Reply-To: References: Message-ID: We use SteriCycle. We decant off all formalin and put the tissue in double red bagged biohazard boxes for incineration. The boxes are hauled by them and we have manifests in triplicate that are retained by our Safety department. Anyone signing the manifest needs to attend the training for the Mass DPH on how to handle haz-waste so you know what you are signing. We keep a binder of the manifests copies and we receive in the mail the original manifest stamped "destroyed" once that is done. We keep all records for three years. I don't know what other states require. I have a procedure to share if you want it. Feel free to reach out. Chuck Bacon, HTL(ASCP)CM Histology Supervisor Baystate Health System 361 Whitney Ave., Holyoke, MA 01040 p:413-322-4786|f: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Piche, Jessica Sent: Tuesday, July 11, 2023 2:01 PM To: Histonet Subject: [Histonet] Tissue disoposal/formalin Good Afternoon, I have a question regarding the dumping of tissue. How are your laboratories dumping tissue when it is time for disposal? Are you putting the specimens with 10% neutral buffered formalin in the waste containers and a company comes and removes them from your facility? I would appreciate any information that you can share. Thank you, Jessica Piche Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From greg.dobbin at gmail.com Wed Jul 12 13:54:24 2023 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Wed, 12 Jul 2023 15:54:24 -0300 Subject: [Histonet] Tisse disposal/formalin Message-ID: Decant formalin off into formalin waste containers and the remaining tissue go into a red bag (red signifying destined for incineration). As the O.R. does with fresh tissues for disposal. *Greg Dobbin* 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 From Jessica.Piche at wtbyhosp.org Thu Jul 13 09:05:32 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 13 Jul 2023 14:05:32 +0000 Subject: [Histonet] Tisse disposal/formalin In-Reply-To: References: Message-ID: Hi Greg, I appreciate your response. Do you do this under a hood? If yes, is it vented outside? Thanks, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ________________________________ From: Greg Dobbin Sent: Wednesday, July 12, 2023 2:54 PM To: Piche, Jessica Cc: histonet at lists.utsouthwestern.edu Subject: RE: Tisse disposal/formalin You don't often get email from greg.dobbin at gmail.com. Learn why this is important [EXTERNAL MSG] Decant formalin off into formalin waste containers and the remaining tissue go into a red bag (red signifying destined for incineration). As the O.R. does with fresh tissues for disposal. Greg Dobbin 1205 Pleasant Grove Rd Route 220 York, PE C0A 1P0 From relia1 at earthlink.net Thu Jul 13 09:17:01 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 13 Jul 2023 10:17:01 -0400 Subject: [Histonet] Back by Popular Demand... Free Resume Tune-ups for my Histopeeps! Message-ID: <00c301d9b594$b1789fc0$1469df40$@earthlink.net> Hi Histopeeps! I hope this is a great week for you! Once again like every Summer, I have been getting emails and calls from your fellow histopeeps asking if I am available to help them with their resumes. The Answer is YES! * Going for a promotion? * Prepping for a performance review? * Preparing for a presentation? * Transitioning from Travel to Permanent work? * Job Hunting? Whatever your reason, it is always a good idea to keep your resume updated. And... I will help you. Let me help you get your resume tuned up!! It's free of charge as a service to my Histopeeps!! Here's what you need to do: Reply to this email with I'm in and we can get started. Job Hunting? Here are some of my Hottest opportunities. All of these are permanent positions. Most are RELIA Exclusives All of my clients offer excellent compensation, benefits and most offer relocation/sign on bonuses!! RELIA Spotlight Opportunity! Greenville, SC Brand NEW LAB!! I am working with a BRAND NEW LAB in beautiful Greenville, SC. They are in need of an experienced certified histotech. This is a Dermpath lab with state of the art equipment and a fantastic group to work with.This is a full time DAY Shift permanent position. RELIA HOT HISTOLOGY Opportunities Locations: * Wisconsin * Arizona * Illinois * South Carolina * Tennessee * California * Florida * Alabama * Georgia * Ohio Areas of Expertise: * Leadership * Quality Assurance * Tech Support * Mohs * HT/HTL And new opportunities are coming in all of the time!! So, if you are looking and none of these appeal to you, shoot me an email and let me know what to keep an eye for; FOR YOU! I look forward to assisting you!! I would like to invite you to join my Facebook group: www.facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker RELIA Solutions E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.facebook.com/PamBarkerRELIA www.linkedin.com/in/reliasolutions #ilovemyhistopeeps #jobs4myhistopeeps #histology #histologylab #histologyjobs #hmuhistopeeps From relia1 at earthlink.net Wed Jul 19 11:29:28 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Wed, 19 Jul 2023 12:29:28 -0400 Subject: [Histonet] Let's chat about how to hire a histotech! "Pick my brain for free" Message-ID: <01bc01d9ba5e$312b3e30$9381ba90$@earthlink.net> Hello Histopeeps!! I hope you?re having a fantastic week and enjoying your summer! A thought occurred to me this morning? I have always offered my candidates guidance on resumes, career strategy, and salary negotiation free of charge so I figured why don?t I offer some help to employers! FREE OF CHARGE!! YES!!!! You read that right! ? I am offering you the opportunity to chat about strategies for hiring histotechs for YOUR lab. ? If you choose to engage me to assist in a search that is fantastic! ? If you just want to pick my brain and sharpen your search capabilities that works too!! Here?s what you need to do: Reply to THIS email with ?I?m IN? ? Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Thu Jul 20 11:06:34 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Thu, 20 Jul 2023 12:06:34 -0400 Subject: [Histonet] Unlock the Secrets of Travel Histology with ME! An update for my histopeeps Message-ID: <02b501d9bb24$28f82c70$7ae88550$@earthlink.net> Hi Histopeeps, I hope you are enjoying your Summer!!! Here's a quick update on the Travel Article... It will be published by the end of the Summer and will cover: * The Pros and Cons of Travel Histology * The Nuts and Bolts of Travel Histology * Some background on Travel Histology * Tips for being successful at Travel Histology * Transitioning out of Travel Histology and into a permanent job I really appreciate your patience as I have been so busy helping your fellow histopeeps find exciting new opportunities that I have not been able to produce the article as quickly as I had originally hoped... Speaking of opportunity... The only thing hotter than the temps this summer are my phone lines!! My phones are ringing off the hook with exciting opportunities. Please take a look at my current openings. All of these positions are full time permanent positions with excellent salaries and great benefits. Some of these clients offer generous SIGN ON BONUSES and most of them are RELIA EXCLUSIVES!! My current openings are located in: * South Carolina * Wisconsin * Florida * Georgia * Alabama * California * Tennessee * Ohio We have new nationwide opportunities coming in daily!! If you or anyone you know might be interested in any of these opportunities please contact me ASAP on my cell at 407-353-5070 or I can be reached at relia1 at earthlink.net or toll free at 866-607-3542. Refer a friend earn a bonus $$$ Thanks-Pam I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals I welcome you to join my group on Facebook: https://facebook.com/groups/histotechnologists www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From monaamin1 at gmail.com Thu Jul 20 16:35:32 2023 From: monaamin1 at gmail.com (Mona Amin) Date: Thu, 20 Jul 2023 17:35:32 -0400 Subject: [Histonet] Prostate biopsy-water logged popping /lost at cutting? Message-ID: Hi everyone, We had a case of prostate core biopsies total 12 blocks First 2 block cut fine and when cutter touched third block lost the core completely. The blocks were on ice face down no longer than 20 mins once faced. The other blocks looked like they were popping out and were taken for re-embedding and then sectioned. Thoughts if this popping out could be because of 1- too much time in water 2- processing issue 3-condesnsation issue from cold plate Generally too we see popping of our biopsies but with this case it was worst. Any help/experiences shared will be greatly appreciated as we try to resolve the root cause. Thank you everyone in advance. From gu.lang at gmx.at Fri Jul 21 07:50:31 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 21 Jul 2023 14:50:31 +0200 Subject: [Histonet] Prostate biopsy-water logged popping /lost at cutting? In-Reply-To: References: Message-ID: <000001d9bbd1$efe595e0$cfb0c1a0$@gmx.at> What first comes in my mind is, that the tissue was a little too cold when placed into the embedding mold. That would cause an insufficient junction with the paraffin. If there was not enough hot paraffin in the embedding mold and handling took a little bit too long, or if the tissue was rather cold and dry before putting it into the mold (cassette too long open before embedding).... something like that. Too brittle tissue because of the processing or because it was airdried before putting it into formalin, tends to pop out of the block. But this would lead to obvious cutting problems. Regards Gudrun -----Urspr?ngliche Nachricht----- Von: Mona Amin via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Donnerstag, 20. Juli 2023 23:36 An: histonet at lists.utsouthwestern.edu Betreff: [Histonet] Prostate biopsy-water logged popping /lost at cutting? Hi everyone, We had a case of prostate core biopsies total 12 blocks First 2 block cut fine and when cutter touched third block lost the core completely. The blocks were on ice face down no longer than 20 mins once faced. The other blocks looked like they were popping out and were taken for re-embedding and then sectioned. Thoughts if this popping out could be because of 1- too much time in water 2- processing issue 3-condesnsation issue from cold plate Generally too we see popping of our biopsies but with this case it was worst. Any help/experiences shared will be greatly appreciated as we try to resolve the root cause. Thank you everyone in advance. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas at biopath.org Fri Jul 21 15:08:33 2023 From: plucas at biopath.org (Paula) Date: Fri, 21 Jul 2023 13:08:33 -0700 Subject: [Histonet] Expiration date after opeing a reagent Message-ID: <003c01d9bc0f$21b67fc0$65237f40$@biopath.org> Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said when the container is opened, the expiration will change and we need to write the date on the container under the opened date. I've never heard of this requirement and I would like to ask if you have. He said if the vendor does not give us any written documentation of when a reagent would expire after it's opened, then we should seek an alternate vendor who can. Any comments, etc. are greatly appreciated. Paula Lucas Bio-Path Medical Group From akbitting at geisinger.edu Fri Jul 21 15:45:45 2023 From: akbitting at geisinger.edu (Bitting, Angela K.) Date: Fri, 21 Jul 2023 20:45:45 +0000 Subject: [Histonet] [External] Expiration date after opeing a reagent In-Reply-To: <003c01d9bc0f$21b67fc0$65237f40$@biopath.org> References: <003c01d9bc0f$21b67fc0$65237f40$@biopath.org> Message-ID: This sounds absurd to me. -----Original Message----- From: Paula via Histonet Sent: Friday, July 21, 2023 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Expiration date after opeing a reagent External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said when the container is opened, the expiration will change and we need to write the date on the container under the opened date. I've never heard of this requirement and I would like to ask if you have. He said if the vendor does not give us any written documentation of when a reagent would expire after it's opened, then we should seek an alternate vendor who can. Any comments, etc. are greatly appreciated. Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. From tpodawiltz at yahoo.com Fri Jul 21 16:30:12 2023 From: tpodawiltz at yahoo.com (Thomas Podawiltz) Date: Fri, 21 Jul 2023 21:30:12 +0000 (UTC) Subject: [Histonet] [External] Expiration date after opeing a reagent In-Reply-To: References: <003c01d9bc0f$21b67fc0$65237f40$@biopath.org> Message-ID: <363975122.2004667.1689975012219@mail.yahoo.com> I think that?inspector has their head up their???. Sent from Yahoo Mail for iPad On Friday, July 21, 2023, 4:45 PM, Bitting, Angela K. via Histonet wrote: This sounds absurd to me. -----Original Message----- From: Paula via Histonet Sent: Friday, July 21, 2023 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Expiration date after opeing a reagent External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said when the container is opened, the expiration will change and we need to write the date on the container under the opened date. I've never heard of this requirement and I would like to ask if you have. He said if the vendor does not give us any written documentation of when a reagent would expire after it's opened, then we should seek an alternate vendor who can. Any comments, etc. are greatly appreciated. Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgonzalez at origene.com Fri Jul 21 18:58:01 2023 From: rgonzalez at origene.com (Rachel Gonzalez) Date: Fri, 21 Jul 2023 23:58:01 +0000 Subject: [Histonet] [External] Expiration date after opeing a reagent In-Reply-To: <363975122.2004667.1689975012219@mail.yahoo.com> References: <003c01d9bc0f$21b67fc0$65237f40$@biopath.org> <363975122.2004667.1689975012219@mail.yahoo.com> Message-ID: Hi I agree the inspector is wrong. I work in the manufacture of pathology reagents such as secondary polymers, diluents, buffers, antibodies, IF conjugates. We constantly test in real time to make sure that the product is as good on its expiration date as it is on day one. We are mostly for RUO, I am sure that the larger companies such as Ventana Dako and Leica are doing the same. To be fair to the inspector maybe he is confusing antibodies that should be kept at -20C and are stored at 4C. Those antibodies will have a shorter half-life. We state this on our inserts with these antibodies. Rachel -----Original Message----- From: Thomas Podawiltz via Histonet Sent: Friday, July 21, 2023 5:30 PM To: Bitting, Angela K. ; Paula ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] [External] Expiration date after opeing a reagent I think that?inspector has their head up their???. Sent from Yahoo Mail for iPad On Friday, July 21, 2023, 4:45 PM, Bitting, Angela K. via Histonet wrote: This sounds absurd to me. -----Original Message----- From: Paula via Histonet Sent: Friday, July 21, 2023 4:09 PM To: histonet at lists.utsouthwestern.edu Subject: [External] [Histonet] Expiration date after opeing a reagent External: Be careful with links and files. If suspicious, alert the Information Security Office. Questions? Call 570-271-8092 Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said when the container is opened, the expiration will change and we need to write the date on the container under the opened date. I've never heard of this requirement and I would like to ask if you have. He said if the vendor does not give us any written documentation of when a reagent would expire after it's opened, then we should seek an alternate vendor who can. Any comments, etc. are greatly appreciated. Paula Lucas Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer The information contained in this communication from the sender is confidential. It is intended solely for use by the recipient and others authorized to receive it. If you are not the recipient, you are hereby notified that any disclosure, copying, distribution or taking action in relation of the contents of this information is strictly prohibited and may be unlawful. This email has been scanned for viruses and malware, and may have been automatically archived by Mimecast Ltd, an innovator in Software as a Service (SaaS) for business. Providing a safer and more useful place for your human generated data. Specializing in; Security, archiving and compliance. To find out more visit the Mimecast website. From samantha.golden at ymail.com Fri Jul 21 20:42:20 2023 From: samantha.golden at ymail.com (Samantha Golden) Date: Fri, 21 Jul 2023 21:42:20 -0400 Subject: [Histonet] Productivity log References: Message-ID: I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? Thank you for sharing your experience. Samantha Sent from my iPhone From tbraud at holyredeemer.com Sat Jul 22 14:48:03 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Sat, 22 Jul 2023 19:48:03 +0000 Subject: [Histonet] changing expiration dates once open Message-ID: <16333e81d9a548c1a0fb6a0172c860dc@holyredeemer.com> The inspector is wrong. Sometimes there is an expiration upon opening, but the majority of reagents do not have this. Just smile and say OK like you really mean it, then check your CLIA regulations and be prepared to back up your defense for the next inspector. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion -----Original Message----- From: histonet-request at lists.utsouthwestern.edu Sent: Saturday, July 22, 2023 1:00 PM Today's Topics 1. Expiration date after opeing a reagent (Paula) Date: Fri, 21 Jul 2023 13:08:33 -0700 From: "Paula" Hello, We just got CLIA inspected and had no deficiencies. The inspector did say he wants to see us write down on any reagent containers when it was opened, and write down the new expiration date. The containers have the expiration date on them from the manufacturer, but the inspector said when the container is opened, the expiration will change and we need to write the date on the container under the opened date. I've never heard of this requirement and I would like to ask if you have. He said if the vendor does not give us any written documentation of when a reagent would expire after it's opened, then we should seek an alternate vendor who can. Any comments, etc. are greatly appreciated. Paula Lucas Bio-Path Medical Group From tbraud at holyredeemer.com Sat Jul 22 15:02:10 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Sat, 22 Jul 2023 20:02:10 +0000 Subject: [Histonet] Logging everything you do. Message-ID: As someone who has been a supervisor in 3 institutions for 35 years, this is not that way to improve productivity. To log in every minute of activity feels very punitive and I can't imagine that it would be well received by staff. The best way to improve productivity is to start by asking each tech on a daily basis what problems do they feel impacts productivity. Some items already have general standard established such as embedding, cutting, staining. Ask your techs for their ideas. I'm sure they will have some valid ones. Don't let the talks dissolve into complaining. Ask for concrete ideas for improvement that can be tried. Look for duplicity in work, records, and labeling. Make sure they have the right tools to do their job. Get on the bench and see it for yourself. There is nothing like first had experience to find the weak spots. They worst way to improve productivity is to require such an onerous demand such as a task log. All you are doing is slowing productivity, not improving. Respectfully, Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion Message: 5 Date: Fri, 21 Jul 2023 21:42:20 -0400 From: Samantha Golden Subject: [Histonet] Productivity log I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? Thank you for sharing your experience. Samantha From patpxs at gmail.com Sat Jul 22 15:36:07 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Sat, 22 Jul 2023 20:36:07 +0000 (UTC) Subject: [Histonet] Logging everything you do. In-Reply-To: References: Message-ID: <1325159208.3175094.1690058167477@mail.yahoo.com> Is this documentation for a work/labor study to determine pricing or show upper leadership how hands-on and time consuming histology is? If not, then what you are asking is punitive.? Not to mention that it will kill any comradery that your histotechs may have.? This will be seen as either a competition or a way to point out those that are not as fast/skilled/etc.? I can hear it now (where I used to work had the histotechs write stuff down for all to see (I stopped that practice right away)? "Betty is picking and choosing her blocks"? "Bob is leaving all the bone blocks for me to do"? "What are YOU going to do about Joe being so slow?"? "Why am I the only one who cuts the prostate biopsies?"? That's only a few of the nicer complaints I heard. Plus it will be a quick way to make for an over all negative environment. I agree with Terri - ask them for to improve, streamline, etc.? Asking the staff for help to improve the lab goes a long way to making a good working environment.? With fewer and fewer people becoming histotechs - the work environment is crucial. Sincerely, Paula Sicurello On Saturday, July 22, 2023 at 01:02:21 PM PDT, Terri Braud via Histonet wrote: As someone who has been a supervisor in 3 institutions for 35 years, this is not that way to improve productivity.? To log in every minute of activity feels very punitive and I can't imagine that it would be well received by staff.? The best way to improve productivity is to start by asking each tech on a daily basis what problems do they feel impacts productivity.? Some items already have general standard established such as embedding, cutting, staining.? Ask your techs for their ideas.? I'm sure they will have some valid ones.? Don't let the talks dissolve into complaining.? Ask for concrete ideas for improvement that can be tried. Look for duplicity in work, records, and labeling.? Make sure they have the right tools to do their job. Get on the bench and see it for yourself.? There is nothing like first had experience to find the weak spots. They worst way to improve productivity is to require such an onerous demand such as a task log.? All you are doing is slowing productivity, not improving. Respectfully, Terri Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion Message: 5 Date: Fri, 21 Jul 2023 21:42:20 -0400 From: Samantha Golden Subject: [Histonet] Productivity log I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? Thank you for sharing your experience. Samantha _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From samantha.golden at ymail.com Sat Jul 22 15:39:37 2023 From: samantha.golden at ymail.com (Samantha Golden) Date: Sat, 22 Jul 2023 16:39:37 -0400 Subject: [Histonet] Logging everything you do. In-Reply-To: References: Message-ID: Thanks for taking the time to respond. It was actually staff who suggested we see what tasks are being performed, when, and how long. No punitive action is being taken. As I indicated, we, as in the team, want to find the pain points. I do continually ask for ideas and feedback; staff repeatedly tell me they cannot see any other way to do things. I am on the bench daily and I know what the problems are. But like you said, I want staff to feel involved in the discussion, and this was their idea. Sent from my iPhone > On Jul 22, 2023, at 4:15 PM, Terri Braud via Histonet wrote: > > ?As someone who has been a supervisor in 3 institutions for 35 years, this is not that way to improve productivity. To log in every minute of activity feels very punitive and I can't imagine that it would be well received by staff. The best way to improve productivity is to start by asking each tech on a daily basis what problems do they feel impacts productivity. Some items already have general standard established such as embedding, cutting, staining. Ask your techs for their ideas. I'm sure they will have some valid ones. Don't let the talks dissolve into complaining. Ask for concrete ideas for improvement that can be tried. Look for duplicity in work, records, and labeling. Make sure they have the right tools to do their job. Get on the bench and see it for yourself. There is nothing like first had experience to find the weak spots. > They worst way to improve productivity is to require such an onerous demand such as a task log. All you are doing is slowing productivity, not improving. > Respectfully, Terri > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-2021 > Honesty > AccouNtability > AgiLity > CoLlaboration > CoMpassion > > > Message: 5 > Date: Fri, 21 Jul 2023 21:42:20 -0400 > From: Samantha Golden > Subject: [Histonet] Productivity log > I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? > Thank you for sharing your experience. > Samantha > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From patpxs at gmail.com Sat Jul 22 18:33:32 2023 From: patpxs at gmail.com (Paula Sicurello) Date: Sat, 22 Jul 2023 23:33:32 +0000 (UTC) Subject: [Histonet] Logging everything you do. In-Reply-To: References: Message-ID: <1254637865.3187738.1690068812593@mail.yahoo.com> I'm glad to hear that the staff suggested the tracking of tasks.? If I had to guess the items causing the most trouble are: Poorly grossed specimens if someone other than a Histotech grosses - all of which cause poor processing:? too big filling the entire cassette; too thick squishing out the top and bottom causing waffling; not decalled long enough needing extra long surface decal to cut. Microtomes:? messed up knife holders with all sorts of dings and dents so the blade doesn't clamp right causing thick & thin sections or chatter;? block clamping head getting out of alignment or full of wax resulting in poor sections.? Cruddy blades - just because it costs less doesn't make it a better blade; knife marks from hitting staples that the grosser swore they took out.?? Waterbath: too cold and getting wrinkles; too hot and sections poof into non-existance.? Floaters from not cleaning the surface between blocks; floaters from someone's skin cells because they don't like wearing gloves when sectioning. Supplies:? or lack of, it seems like there may still be supply chain issues.?? Everyone's favorite:? clinicians calling asking why it's taking so long to get results.? This week I had one tell me she got her flow results in a day and Histo has had the specimens for two days - so what's the hold up? Please share what items are troubling after the lists are compiled. It will be interesting to see what they have to say.? Sincerely, Paula Sicurello On Sat, Jul 22, 2023 at 1:39 PM, Samantha Golden via Histonet wrote: Thanks for taking the time to respond. It was actually staff who suggested we see what tasks are being performed, when, and how long. No punitive action is being taken. As I indicated, we, as in the team, want to find the pain points. I do continually ask for ideas and feedback; staff repeatedly tell me they cannot see any other way to do things. I am on the bench daily and I know what the problems are. But like you said, I want staff to feel involved in the discussion, and this was their idea. Sent from my iPhone > On Jul 22, 2023, at 4:15 PM, Terri Braud via Histonet wrote: > > ?As someone who has been a supervisor in 3 institutions for 35 years, this is not that way to improve productivity.? To log in every minute of activity feels very punitive and I can't imagine that it would be well received by staff.? The best way to improve productivity is to start by asking each tech on a daily basis what problems do they feel impacts productivity.? Some items already have general standard established such as embedding, cutting, staining.? Ask your techs for their ideas.? I'm sure they will have some valid ones.? Don't let the talks dissolve into complaining.? Ask for concrete ideas for improvement that can be tried. Look for duplicity in work, records, and labeling.? Make sure they have the right tools to do their job. Get on the bench and see it for yourself.? There is nothing like first had experience to find the weak spots. > They worst way to improve productivity is to require such an onerous demand such as a task log.? All you are doing is slowing productivity, not improving. > Respectfully, Terri > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-2021 >? ? ? ? ? Honesty > AccouNtability >? ? AgiLity >? ? CoLlaboration >? CoMpassion > > > Message: 5 > Date: Fri, 21 Jul 2023 21:42:20 -0400 > From: Samantha Golden > Subject: [Histonet] Productivity log > I have asked staff to start logging all the tasks they perform and the amount of time it is taking them to complete. We would like to identify pain points and waste in an effort to improve our overall productivity. Rather than reinventing the wheel, does someone have a form they?ve used in the past that they would be willing to share? > Thank you for sharing your experience. > Samantha > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From relia1 at earthlink.net Tue Jul 25 11:31:45 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 25 Jul 2023 12:31:45 -0400 Subject: [Histonet] RELIA HOT JOB ALERT!!! Brand NEW Lab in TAMPA Florida and more exciting opportunities Nationwide! Message-ID: <18de01d9bf15$80e0b9d0$82a22d70$@earthlink.net> Hello Histopeeps, How are you? I hope you are having a wonderful day and are looking forward to a great week! I have an exciting opportunity in Florida in a BRAND NEW LAB!! If you or someone you know are ASCP Certified and interested, we need to talk. I am working with one of my BEST clients in Illinois that are in need of histopeeps like you! My clients is offering an exciting opportunity that is a permanent full-time position with excellent compensation and benefits This opportunity is located in Tampa, FL Histopeeps, would you be interested in in this opportunity? OR Do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas please let me know. I am getting calls for histology professionals at all levels practically everywhere on almost DAILY basis!! I currently have other exciting opportunities in: * Florida * California * South Carolina * Tennessee * Georgia * Alabama * Wisconsin I invite you to join my group on Facebook: www.facebook.com/groups/histotechnologists Have a Great Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From relia1 at earthlink.net Tue Jul 25 11:40:40 2023 From: relia1 at earthlink.net (relia1 at earthlink.net) Date: Tue, 25 Jul 2023 12:40:40 -0400 Subject: [Histonet] Technical Glitch and repost!! RELIA HOT JOB ALERT ! Brand NEW LAB in Tampa and other exciting NEW opportunities. Can you help? Message-ID: <18fa01d9bf16$bfdeb690$3f9c23b0$@earthlink.net> Hello Histopeeps, How are you? I hope you are having a wonderful day and are looking forward to a great week! I have an exciting opportunity in Florida in a BRAND NEW LAB!! If you or someone you know are ASCP Certified and interested, we need to talk. I am working with one of my BEST clients in FLORIDA that is in need of histopeeps like you! My clients is offering an exciting opportunity that is a permanent full-time position with excellent compensation and benefits This opportunity is in Tampa, FL Histopeeps, would you be interested in in this opportunity? OR Do you know of anyone who might be interested in this position? I really appreciate you taking the time to read this e-mail and it means a lot to me when you take the time to refer your friends and coworkers so to show you my appreciation, I would like to offer you a 250.00 referral fee for anyone you refer to me that I place. So if you think you or someone you know might be interested please contact me. I can be reached at 866-607-3542, on my cell at 407-353-5070 or relia1 at earthlink.net If you are contemplating a move but NOT to one of these areas please let me know. I am getting calls for histology professionals at all levels practically everywhere on almost DAILY basis!! I currently have other exciting opportunities in: * Florida * California * South Carolina * Tennessee * Georgia * Alabama * Wisconsin I invite you to join my group on Facebook: www.facebook.com/groups/histotechnologists Have a Great Week! Thanks-Pam Right Time, Right Place, Right Move with RELIA! Providing excellent service exclusively to the Histology Community! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5717 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 Toll free: (866)60RELIA or (866)607-3542 E-mail: relia1 at earthlink.net https://www.facebook.com/RELIASolutionsforhistologyprofessionals www.linkedin.com/in/reliasolutions Follow my hashtags to make your day great and your career greater! #ilovemyhistopeeps #jobs4myhistopeeps #histologyiscool #histologyjobs #histologycareers #histology From criley at udel.edu Wed Jul 26 13:23:16 2023 From: criley at udel.edu (Charles Riley) Date: Wed, 26 Jul 2023 14:23:16 -0400 Subject: [Histonet] Von Kossa staining Message-ID: Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? From DSiena at statlab.com Wed Jul 26 20:49:15 2023 From: DSiena at statlab.com (Debra Siena) Date: Thu, 27 Jul 2023 01:49:15 +0000 Subject: [Histonet] Von Kossa staining In-Reply-To: References: Message-ID: Hi Charles, I can't answer your question about UV light as I've never tried it except with incandescent lights. However, I do know that you could use natural sunlight as well to develop the silver color change. I hope that helps, Thanks Debbie Siena, HT(ASCP)QIHC Director of Scientific Affairs Mobile:817-994-9407 Dallas, TX | Baltimore, MD | Mt. Vernon, WA -----Original Message----- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 1:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining *** Externally sourced email message *** Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CAUTION: This email was originated from outside StatLab and contains links. Do not click links or open attachments unless you recognize the sender and you know the content is safe. From jkiernan at uwo.ca Wed Jul 26 23:50:49 2023 From: jkiernan at uwo.ca (John Kiernan) Date: Thu, 27 Jul 2023 04:50:49 +0000 Subject: [Histonet] Von Kossa staining In-Reply-To: References: Message-ID: Charles, A handheld light of any kind isn't really suitable because you would have to hold it over the slides for 15 to 60 minutes, according to which variant of the von Kossa method you plan to use (see Lillie & Fullmer 1976 Histopathologic Technic ... 4th ed. pp 539-541). An anglepoise lamp with an old-fashioned 100W bulb is OK, and so is a sunny windowsill. Silver salts absorb at the blue end of the spectrum, so a fluorescent light should be more efficient than an incandescent bulb. If no bright light source is available, it's possible to chemically reduce the silver phosphate and/or carbonate to black colloidal silver, with a traditional photographic developer. The method of Rungby et al.1993 may be better than other post-reduction methods (https://scholar.google.ca/scholar?hl=en&as_sdt=0%2C5&q=rungby+1993+calcium+deposits&oq=rungby+1993). I never tried it, but Rungby's paper has collected 104 citations, which is very good for a paper in our field. The von Kossa technique is simply explained in my Histological and Histochemical Methods textbook, 5th edn (2015). The book costs less than 1ml of any antibody. Enough said! John John A. Kiernan Emeritus, Anatomy & Cell Biology, University of Western Ontario London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html Also Secretary, Biological Stain Commission, Inc. https://biologicalstaincommission.org = = = ________________________________ -----Original Message----- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 1:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining *** Externally sourced email message *** Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CAUTION: This email was originated from outside StatLab and contains links. Do not click links or open attachments unless you recognize the sender and you know the content is safe. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b-frederick at northwestern.edu Thu Jul 27 08:22:50 2023 From: b-frederick at northwestern.edu (Bernice Frederick) Date: Thu, 27 Jul 2023 13:22:50 +0000 Subject: [Histonet] Von Kossa staining In-Reply-To: References: Message-ID: I use a 60 watt bulb and it works fine. Bernice -----Original Message----- From: John Kiernan via Histonet Sent: Wednesday, July 26, 2023 11:51 PM To: Charles Riley ; histonet at lists.utsouthwestern.edu; dsiena Subject: Re: [Histonet] Von Kossa staining Charles, A handheld light of any kind isn't really suitable because you would have to hold it over the slides for 15 to 60 minutes, according to which variant of the von Kossa method you plan to use (see Lillie & Fullmer 1976 Histopathologic Technic ... 4th ed. pp 539-541). An anglepoise lamp with an old-fashioned 100W bulb is OK, and so is a sunny windowsill. Silver salts absorb at the blue end of the spectrum, so a fluorescent light should be more efficient than an incandescent bulb. If no bright light source is available, it's possible to chemically reduce the silver phosphate and/or carbonate to black colloidal silver, with a traditional photographic developer. The method of Rungby et al.1993 may be better than other post-reduction methods (https://urldefense.com/v3/__https://scholar.google.ca/scholar?hl=en&as_sdt=0*2C5&q=rungby*1993*calcium*deposits&oq=rungby*1993__;JSsrKys!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfChgoE5Zi$ ). I never tried it, but Rungby's paper has collected 104 citations, which is very good for a paper in our field. The von Kossa technique is simply explained in my Histological and Histochemical Methods textbook, 5th edn (2015). The book costs less than 1ml of any antibody. Enough said! John John A. Kiernan Emeritus, Anatomy & Cell Biology, University of Western Ontario London, Canada https://urldefense.com/v3/__https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCiLlysL2$ Also Secretary, Biological Stain Commission, Inc. https://urldefense.com/v3/__https://biologicalstaincommission.org__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCtBsy2U8$ = = = ________________________________ -----Original Message----- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 1:23 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining *** Externally sourced email message *** Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ CAUTION: This email was originated from outside StatLab and contains links. Do not click links or open attachments unless you recognize the sender and you know the content is safe. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Dq0X2DkFhyF93HkjWTBQKhk!SGP1gx0IglGW5nnid6Tbv6aRuFAWHsIiz6kn3jDzSw7njXRiBLURbNbbv4YCJKl-Jbcpm60EOukGFQIdqh2rLhqFIgrWGAWfCgGN6_75$ From jmacdonald at mtsac.edu Thu Jul 27 11:44:18 2023 From: jmacdonald at mtsac.edu (Mac Donald, Jennifer) Date: Thu, 27 Jul 2023 16:44:18 +0000 Subject: [Histonet] Von Kossa staining In-Reply-To: References: Message-ID: I have used a UV light like the ones for indoor plants and one that is in the fume hood for disinfection. Both proved successful. Jennifer -----Original Message----- From: Charles Riley via Histonet Sent: Wednesday, July 26, 2023 11:23 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining EXTERNAL SENDER - Exercise caution with requests, links, and attachments. Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud at holyredeemer.com Thu Jul 27 12:04:46 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 27 Jul 2023 17:04:46 +0000 Subject: [Histonet] Von Kossa light source Message-ID: We've used the UV light for the hood disinfectant, as well as just taken a break out in the sunshine. Both worked just fine Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion From TNMayer at mdanderson.org Thu Jul 27 12:46:29 2023 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Thu, 27 Jul 2023 17:46:29 +0000 Subject: [Histonet] Von Kossa staining In-Reply-To: References: Message-ID: Charles, I am going to date myself and tell you that we placed the glass coplin jar on the back of the embedder (or in the water bath) added a bankers light and wrapped it with foil for 1 hr. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Asst Professor/ Assoc Program Director HTL Program UTMDACC tnmayer at mdanderson.edu off cell: 832-710-1837 off: 713-563-3481 Today's Topics: 1. Von Kossa staining (Charles Riley) 2. Re: Von Kossa staining (Debra Siena) 3. Re: Von Kossa staining (John Kiernan) 4. Re: Von Kossa staining (Bernice Frederick) 5. Re: Von Kossa staining (Mac Donald, Jennifer) ---------------------------------------------------------------------- Message: 1 Date: Wed, 26 Jul 2023 14:23:16 -0400 From: Charles Riley To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Von Kossa staining Message-ID: Content-Type: text/plain; charset="UTF-8" Can anyone out there who performs Von Kossa staining provide me with any guidelines or suggestions for the light source to use for the Silver nitrate activation? Is a standard handheld black light strong enough or does it need to be a UV sanitizing strength light if using UV versus incandescent bulb exposure? ------------------------------ The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. 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