From cmcgrad1 at hurleymc.com Thu Jan 5 10:07:39 2023 From: cmcgrad1 at hurleymc.com (Cynthia McGrady) Date: Thu, 5 Jan 2023 11:07:39 -0500 Subject: [Histonet] control slides Message-ID: Hello. Hoping someone can help me. Typically we order TAG 72 control slides from Cell Marque.(our Docs are unhappy with our in-house control tissue, we've tried). Cell Marque is on B/O no ETA. So my backup is Cancer Diagnostics, they are 2-3 weeks out to fill our order. Any other companies out there that sell TAG 72 control slides that I don't know about? Any help would be greatly appreciated. Thanks..Cindy Cindy McGrady HT(ASCP) QIHC Hurley Medical Center One Hurley Plaza Flint, Mi 48503 810-262-9138 -- If you are not the intended recipient(s), you are notified that any disclosure, copying, distribution or any action taken or omitted to be taken in reliance on the contents of this information is prohibited and may be unlawful. If you receive this message in error, or are not the named recipient(s), please notify the sender, delete this e-mail from your computer, and destroy any copies in any form immediately. From vtolley25 at gmail.com Thu Jan 5 10:37:58 2023 From: vtolley25 at gmail.com (Val Tolley) Date: Thu, 5 Jan 2023 08:37:58 -0800 Subject: [Histonet] control slides In-Reply-To: References: Message-ID: <091E24B1-8F46-47B2-B15F-447209D4BE99@gmail.com> Hey Cynthia- I?m curious what you used for an in-house control? I bet you have some in-house tissue that will work as well as the purchased controls. Try out a big piece or lung or colon adenocarcinoma from a resection, if you can. Val > On Jan 5, 2023, at 8:23 AM, Cynthia McGrady via Histonet wrote: > > ?Hello. > Hoping someone can help me. Typically we order TAG 72 control slides from > Cell Marque.(our Docs are unhappy with our in-house control tissue, we've > tried). Cell Marque is on B/O no ETA. So my backup is Cancer Diagnostics, > they are 2-3 weeks out to fill our order. Any other companies out there > that sell TAG 72 control slides that I don't know about? Any help would be > greatly appreciated. Thanks..Cindy > Cindy McGrady > HT(ASCP) QIHC > Hurley Medical Center > One Hurley Plaza > Flint, Mi 48503 > 810-262-9138 > > -- > > > If you are not the intended > recipient(s), you are notified that any > disclosure, copying, distribution or > any action taken or omitted to be > taken in reliance on the contents of this > information is prohibited and may > be unlawful. If you receive this message in > error, or are not the named > recipient(s), please notify the sender, delete this > e-mail from your > computer, and destroy any copies in any form immediately. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kdean70 at hotmail.com Tue Jan 10 11:14:21 2023 From: kdean70 at hotmail.com (Ken M) Date: Tue, 10 Jan 2023 17:14:21 +0000 Subject: [Histonet] Tech Needed Message-ID: We are looking for a part-time technician for our fast growing Durham, NC lab. A retired Histotech would be ideal. No live patients or grossing. Mostly cutting and staining processed and embedded blocks. Some IHC experience would be great, but not necessary. Contact Helena at Hmurong at springsidesci.com for details. Ken Springside scientific From Nancy.Schmitt at mercyhealth.com Tue Jan 10 15:33:59 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Tue, 10 Jan 2023 21:33:59 +0000 Subject: [Histonet] Moh's training Message-ID: Hello- Looking for info. On Moh's training - did you go through the ASMH? Trained by Dermatologist or pathologist? Are you HT, HTL or uncertified? I will appreciate any thoughts on this - a new opportunity for us; we currently do Frozen Section cutting, but not Moh's. Thank You Nancy Schmitt, MLT(ASCP) CM HT CM Pathology Support Services Supervisor Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From techmana12 at yahoo.com Thu Jan 12 12:01:39 2023 From: techmana12 at yahoo.com (Dorothy Glass) Date: Thu, 12 Jan 2023 18:01:39 +0000 (UTC) Subject: [Histonet] BLOCKS AND SLIDES References: <2133152761.579610.1673546499433.ref@mail.yahoo.com> Message-ID: <2133152761.579610.1673546499433@mail.yahoo.com> What is the required length of time that you must keep blocks and slides in histology From smclaughlin7 at cooleydickinson.org Thu Jan 12 12:22:20 2023 From: smclaughlin7 at cooleydickinson.org (McLaughlin, Stacy L.) Date: Thu, 12 Jan 2023 18:22:20 +0000 Subject: [Histonet] BLOCKS AND SLIDES In-Reply-To: <2133152761.579610.1673546499433@mail.yahoo.com> References: <2133152761.579610.1673546499433.ref@mail.yahoo.com> <2133152761.579610.1673546499433@mail.yahoo.com> Message-ID: The CAP requirement is a minimum of 10 years for both. Your state's department of health may have different standards. The strictest standard should always be adhered to. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughlin7 at cooleydickinson.org -----Original Message----- From: Dorothy Glass via Histonet Sent: Thursday, January 12, 2023 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] BLOCKS AND SLIDES External Email - Use Caution What is the required length of time that you must keep blocks and slides in histology _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/12ymiHMmziE5ra4ctn7Iwh-cs1v6tsimMbb2VotphRFEiNUqzAT7iNCbxg2jR_bRlOdEuj2P6KrezNMxkouJ_NcxdqJ9aplPCC_X6OraquaP4FRbr0oU4Ii0A3HDQF7HN7bSR3W8TvZ7qdKhr9_GQXh9cTaoY8tU6UGGaAQJGNQ6HKm4BE1o0LSCTOlvGhf-D-iloBowJ7QLyvIjWuZXGqONLozDtm-0PrVjSKn2Uqpb2YN-7gX26DlojXaXT5Cc6zb_kyuYfrf-mmJ0NiaMkZdx7Ud6B_4sl_D-dHe3VQTLF1QLyg0mYs8c-rtULo0QJr7fgZSXHLMozTVcQcE_Hdg/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Mass General Brigham Compliance HelpLine at https://www.massgeneralbrigham.org/complianceline . Please note that this e-mail is not secure (encrypted). If you do not wish to continue communication over unencrypted e-mail, please notify the sender of this message immediately. Continuing to send or respond to e-mail after receiving this message means you understand and accept this risk and wish to continue to communicate over unencrypted e-mail. From Bonnie.Whitaker at osumc.edu Thu Jan 12 12:45:10 2023 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Thu, 12 Jan 2023 18:45:10 +0000 Subject: [Histonet] BLOCKS AND SLIDES In-Reply-To: <2133152761.579610.1673546499433@mail.yahoo.com> References: <2133152761.579610.1673546499433.ref@mail.yahoo.com> <2133152761.579610.1673546499433@mail.yahoo.com> Message-ID: It depends on who your most stringent governing body is. If you have a lab in NY, or that takes specimens from NY, it's 20 years for both. For CAP it's 10 years. There may be other states that have more stringent rules, etc. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Dorothy Glass via Histonet Sent: Thursday, January 12, 2023 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] BLOCKS AND SLIDES External Email: CAUTION What is the required length of time that you must keep blocks and slides in histology _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!AcgyE0lytWhQ93VpOoSaZQdMsM7msm3OiqWcJpTtgOxrg8ezN9A4imEm81YtEO98K2qFpE_RSeeKIOkvuWzaStjryx-KdVumiMnE_g$ From kdean70 at hotmail.com Thu Jan 12 14:43:53 2023 From: kdean70 at hotmail.com (Ken M) Date: Thu, 12 Jan 2023 20:43:53 +0000 Subject: [Histonet] Expired Tissue Blocks Message-ID: Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. From Bonnie.Whitaker at osumc.edu Thu Jan 12 15:05:06 2023 From: Bonnie.Whitaker at osumc.edu (Whitaker, Bonnie) Date: Thu, 12 Jan 2023 21:05:06 +0000 Subject: [Histonet] Expired Tissue Blocks In-Reply-To: References: Message-ID: Caution to all--make sure your patients have signed consent forms that will include this use of their tissue. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Ken M via Histonet Sent: Thursday, January 12, 2023 3:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Expired Tissue Blocks External Email: CAUTION Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!G28o9Kc7mldDguiyt-GBNAuODGNByP6jiHkEV_WQcSZkzxznBtLMC1hfOWgZLKZ8W5auSSJzH2-kuOJ4Hzt4Ep4aitSlSJ5LvlUfyw$ From kdean70 at hotmail.com Thu Jan 12 15:36:05 2023 From: kdean70 at hotmail.com (Ken M) Date: Thu, 12 Jan 2023 21:36:05 +0000 Subject: [Histonet] Tissue blocks Message-ID: If that were true, all cancer research and antibody development in the USA would come to a complete halt. Get a signature from someone who died of cancer 10 years ago?? Here are the findings of most US courts. Here are the facts. In legal disputes concerning the research use of excised tissues, courts consistently have rejected the idea that patients are the owners of their excised tissues or retain any property rights. While only three cases have been adjudicated to date, the findings (in both state and federal courts) have been consistent: patients do not have individual ownership, retain property rights, or have complete control over research use of their excised tissues.9, Contact your legal Counsil to find the tructh. We do not want any patient identifiers or case numbers. From kdean70 at hotmail.com Thu Jan 12 15:54:50 2023 From: kdean70 at hotmail.com (Ken M) Date: Thu, 12 Jan 2023 21:54:50 +0000 Subject: [Histonet] Expired Tissue Blocks In-Reply-To: References: Message-ID: The law is the law in the USA. So far the unanoumous decisions across the USA courts have been very clear. For those who are being super cautious despite the prevailing laws of the USA, You are doing very little to help develop new antibodies and enhance cancer research. ________________________________ From: Whitaker, Bonnie via Histonet Sent: Thursday, January 12, 2023 9:05 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Expired Tissue Blocks Caution to all--make sure your patients have signed consent forms that will include this use of their tissue. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Ken M via Histonet Sent: Thursday, January 12, 2023 3:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Expired Tissue Blocks External Email: CAUTION Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=https%3A%2F%2Furldefense.com%2Fv3%2F__http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet__%3B!!AU3bcTlGKuA!G28o9Kc7mldDguiyt-GBNAuODGNByP6jiHkEV_WQcSZkzxznBtLMC1hfOWgZLKZ8W5auSSJzH2-kuOJ4Hzt4Ep4aitSlSJ5LvlUfyw%24&data=05%7C01%7C%7Ccf3618d662ec452bf6fe08daf4e19134%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C638091546887356325%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=7YmaySdyhjLkT797JDAEWdmSCvBNYPaiWOuFtNrYH9A%3D&reserved=0 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://na01.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7C%7Ccf3618d662ec452bf6fe08daf4e19134%7C84df9e7fe9f640afb435aaaaaaaaaaaa%7C1%7C0%7C638091546887356325%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=npRxLEwmB8vLkgpIKybAzEZs%2BAjfDVTHTHOGJsP8EJQ%3D&reserved=0 From Jeffrey.Rinker at SanfordHealth.org Mon Jan 16 13:07:29 2023 From: Jeffrey.Rinker at SanfordHealth.org (Rinker,Jeffrey) Date: Mon, 16 Jan 2023 19:07:29 +0000 Subject: [Histonet] Job posting Message-ID: We now have a job opening in Bemidji Minnasota. Small lab great people. ----------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain privileged and confidential information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mroark at sfmc.net Wed Jan 18 10:41:33 2023 From: mroark at sfmc.net (Matthew D. Roark) Date: Wed, 18 Jan 2023 16:41:33 +0000 Subject: [Histonet] Temperature Monitoring System Message-ID: Good morning all! Is there a wireless temperature monitoring system that you would recommend? Matthew Roark, HTL(ASCP)CM Histology Specialist Laboratory P 573-331-3982 | M 573-979-1925 | F 573-331-5049 mroark at sfmc.net Saint Francis Healthcare System 211 Saint Francis Drive Cape Girardeau, MO 63703 [https://www.sfmc.net/images/SFHC_logo_email_signature.png] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ? From bcooper at chla.usc.edu Wed Jan 18 11:24:18 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 18 Jan 2023 17:24:18 +0000 Subject: [Histonet] Temperature Monitoring System In-Reply-To: References: Message-ID: <1881aa30894e45b58765ce2582f8e2be@chla.usc.edu> We use Isensix here, and I really like the system. It's not exactly "wireless" because each unit is hardwired (with AA battery backup) into a CP unit which transmits relays data back to an Access Point (also hard wired), which in turn relays the information to the Control Center software. When a unit goes out of range, the system can email designated individuals or page specific numbers, and has backup notifications to designated individuals if the primary contact doesn't respond within a designated number of notifications sent. They are "wireless" in the sense that there aren't wires running through ceilings back to a single central location, but there are certainly visible wires wherever a CP or AP is located. We monitor refrigerators, freezers, room temp and humidity in countless sections of the pathology lab, and even elsewhere in the hospital here. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Matthew D. Roark via Histonet Sent: Wednesday, January 18, 2023 8:42 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Temperature Monitoring System (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good morning all! Is there a wireless temperature monitoring system that you would recommend? Matthew Roark, HTL(ASCP)CM Histology Specialist Laboratory P 573-331-3982 | M 573-979-1925 | F 573-331-5049 mroark at sfmc.net Saint Francis Healthcare System 211 Saint Francis Drive Cape Girardeau, MO 63703 [https://secure-web.cisco.com/1oYDnRYofx9bDERNuRkC14hnaPlIsSymJHrUVz0-C93rc_UyfDYAM7uofojj0YG6UxbIO6z9vuW3zz-512saarod0IXt4o99hy48YDvxhrYh95z7tIs3SaC332CSOE2EC1nqkn1V88TvfjTVjCI33mqhPa8ksx6QtlJYq51ZGt8YvSG_r8bXojpoQ1pU6pWfYxIM76laz2FSi3HfwRceWsXZGehxvlajZ4s0Xb4pmxA1J95iHjleXjfPmhLt1dXbzGTOINTNW66KLGfCgnfVSaNZZdaBNVao7hm3WWJurjNqggh-wexkTcxYsR8PxH7eAl_sPEEMGSvODQ_rnrBFS8CVabMKRvPv5QLGiWF0xaMTZu7PCYgBRFDcVTZJT6d7TY0W0AQcYKfNv5lu1EI7r8RTmz6X7-HWwMTS0klKkvFWvPEettWHhDGnvChwbIg65/https%3A%2F%2Fwww.sfmc.net%2Fimages%2FSFHC_logo_email_signature.png] Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ? _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1fAissDcn1M2CHWNg3iDF_bIGOkw0hv68C3hCm4fUArySyoMlLcYstcXb95pIGuoVmnlGropefwJk-z6YHNwpq3Lm9RKG6NwXQgt3xSOFIFqA593StWf0o_sPZno3n3-sG_kr0gElCGZ3tRXdBH0SJEtguQxnAEJo5DDCjv08eqJjR3Xol5fwuzUMyQQwAvNL1JUdbNN7kS4LhMv5fApQ6S6N02O_C7qBMDtPAxfmn2bn6hLhHWx-5dDTWwP6MXz87GLWjwwPk8dgHxWpuvaL1pFWRYXuNz9znGBNQh782kby-wb2t28ouxAEzovhdpl1rHO2VZiG28v6Gz79u6lA7YElGofbMre-b8BY0dx5dkjFyUIhYK7S1eRVY8jVohfLvF-WSK5Yrb-nxjNyEgjZ9uAMgatWSFoxVTyw8hIT3JlXMR3DEWSsKSh1C9xDF_-5e_a3TadJ5rhjzw-YfCAykQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From edmartin26 at gmail.com Wed Jan 18 14:10:58 2023 From: edmartin26 at gmail.com (Eddie Martin) Date: Wed, 18 Jan 2023 15:10:58 -0500 Subject: [Histonet] Suggest a Temperature Monitoring System In-Reply-To: References: Message-ID: Hi Matthew, Our laboratory performs clinical testing that supports the NIH Clinic Center. The current temperature monitoring system we?re using is acquired through ReesScientific.com The software is easy to use regardless of the extent of privileges a user has. The easiest advantage over freshloc I can think of is an option to find tagged equipment on a map within the lab. That feature is more helpful on 2nd and 3rd shift, or in the event a lab section is being renovated and equipment isn?t in its usual location. Best regards. Very respectfully, Eddie Martin > On Jan 18, 2023, at 11:41 AM, Matthew D. Roark wrote: > > ?Good morning all! > > Is there a wireless temperature monitoring system that you would recommend? > > > > > > Matthew Roark, HTL(ASCP)CM > Histology Specialist > Laboratory > > P 573-331-3982 | M 573-979-1925 | F 573-331-5049 > mroark at sfmc.net > > Saint Francis Healthcare System > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > > [https://www.sfmc.net/images/SFHC_logo_email_signature.png] > > Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > ? > From Emmanuel-Geoffre.S.Gonzaga at kp.org Wed Jan 18 15:00:38 2023 From: Emmanuel-Geoffre.S.Gonzaga at kp.org (Emmanuel Geoffrey S. Gonzaga) Date: Wed, 18 Jan 2023 21:00:38 +0000 Subject: [Histonet] Suggest a Temperature Monitoring System In-Reply-To: References: Message-ID: Hello Matthew, Our lab is using Viewpoint from Mesalabs. Very easy to use and can monitor both temperature and humidity. -------- Emmanuel Gonzaga Operations Manager (Histology/IHC) Kaiser Permanente Histology Department- Chino Hills 13000 Peyton Drive Chino Hills, CA 91709 (909) 703-6931 (Office) / 263 (tie-line) (909) 703-6924 (Lab) (909) 703-6080 (Fax) --------- kp.org/thrive Upcoming PTO: -----Original Message----- From: Eddie Martin via Histonet Sent: Wednesday, January 18, 2023 12:11 PM To: Matthew D. Roark Cc: histonet at lists.utsouthwestern.edu Subject: [Histonet] Suggest a Temperature Monitoring System Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ Hi Matthew, Our laboratory performs clinical testing that supports the NIH Clinic Center. The current temperature monitoring system we?re using is acquired through ReesScientific.com The software is easy to use regardless of the extent of privileges a user has. The easiest advantage over freshloc I can think of is an option to find tagged equipment on a map within the lab. That feature is more helpful on 2nd and 3rd shift, or in the event a lab section is being renovated and equipment isn?t in its usual location. Best regards. Very respectfully, Eddie Martin > On Jan 18, 2023, at 11:41 AM, Matthew D. Roark wrote: > > ?Good morning all! > > Is there a wireless temperature monitoring system that you would recommend? > > > > > > Matthew Roark, HTL(ASCP)CM > Histology Specialist > Laboratory > > P 573-331-3982 | M 573-979-1925 | F 573-331-5049 > mroark at sfmc.net > > Saint Francis Healthcare System > 211 Saint Francis Drive > Cape Girardeau, MO 63703 > > [https://urldefense.com/v3/__https://www.sfmc.net/images/SFHC_logo_ema > il_signature.png__;!!BZ50a36bapWJ!sjxR66-5jcdI2cmo1dnXXg7FkpzH8VZ6ZLyD > 1XPyAFZAzzpsT8TSPrJyRs2-LdUIRVzlUZ8ROAlZIFSjQvvGF9Xam8a_P60ueoBv8unbot > I-$ > ] jxR66-5jcdI2cmo1dnXXg7FkpzH8VZ6ZLyD1XPyAFZAzzpsT8TSPrJyRs2-LdUIRVzlUZ8 > ROAlZIFSjQvvGF9Xam8a_P60ueoBv8tE4CD9O$ > > > Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. > > ? > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!sjxR66-5jcdI2cmo1dnXXg7FkpzH8VZ6ZLyD1XPyAFZAzzpsT8TSPrJyRs2-LdUIRVzlUZ8ROAlZIFSjQvvGF9Xam8a_P60ueoBv8oYy9krz$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. From Kelly.Pairan at nationwidechildrens.org Fri Jan 20 08:29:38 2023 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Fri, 20 Jan 2023 14:29:38 +0000 Subject: [Histonet] HSP 70 or Heat Shock Protein Message-ID: Good Morning and Happy Friday, One of our pathologists is looking for a clinical lab that is doing staining for HSP 70 or Heat Shock Protein. Are any of your labs doing that as a clinical test? Thanks, Kelly Kelly Pairan, BS, HT (ASCP)CM, QIHCCM (ASCP) Technical Specialist -Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From Christopher.Sheeder at seattlechildrens.org Fri Jan 20 08:53:55 2023 From: Christopher.Sheeder at seattlechildrens.org (Sheeder, Christopher) Date: Fri, 20 Jan 2023 14:53:55 +0000 Subject: [Histonet] AFB and Spirochetes slides needed Message-ID: Good morning all, I am validating new stains and looking for 4-5 slides each of AFB+ and Spirochetes+. I am willing to trade for something you need. Email me directly if you want to work something out. Regards- Christopher Sheeder, HT(ASCP)CMQIHC Department of Laboratories Seattle Children's Hospital 4800 Sand Point Way NE Seattle, WA 98105 Office: 206-987-6259 christopher.sheeder at seattlechildrens.org WWW seattlechildrens.org COMPASSION | EXCELLENCE | INTEGRITY | COLLABORATION | EQUITY | INNOVATION CONFIDENTIALITY NOTICE: This e-mail, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From modz9636 at gmail.com Fri Jan 20 12:53:59 2023 From: modz9636 at gmail.com (M.O.) Date: Fri, 20 Jan 2023 10:53:59 -0800 Subject: [Histonet] Looking for Bone/Pathology saw recommendations Message-ID: Hello and Happy Friday! I work in an arthritis research lab and we are looking into pathology saws like the Exakt 302. We study lumbar spine and knees. During our tissue harvesting procedure we take slabs of each vertebral disc unit, facet joints, and knee osteochondral samples. Does anyone have experience in cutting bone specimens with a saw like this? Do you have any recommendations on the brand you prefer? Sincerely, Merissa From nmargaryan88 at gmail.com Fri Jan 20 17:59:50 2023 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Fri, 20 Jan 2023 17:59:50 -0600 Subject: [Histonet] Looking for Bone/Pathology saw recommendations In-Reply-To: References: Message-ID: Yes, I use it for almost a year and I love it. Only thing is that you have to take good care of the unit. If you have more questions I will be happy to answer, Naira On Fri, Jan 20, 2023 at 1:07 PM M.O. via Histonet < histonet at lists.utsouthwestern.edu> wrote: > Hello and Happy Friday! > I work in an arthritis research lab and we are looking into pathology saws > like the Exakt 302. We study lumbar spine and knees. During our tissue > harvesting procedure we take slabs of each vertebral disc unit, facet > joints, and knee osteochondral samples. Does anyone have experience in > cutting bone specimens with a saw like this? Do you have any > recommendations on the brand you prefer? > > Sincerely, > Merissa > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jmjohnson34 at hotmail.com Sat Jan 21 13:14:14 2023 From: jmjohnson34 at hotmail.com (Jennifer Johnson) Date: Sat, 21 Jan 2023 19:14:14 +0000 Subject: [Histonet] Chatter in sections Message-ID: We recently switched paraffin and our pathologist is complaining of chatter in sections. My coworker and I split blocks and some sections are cut by her and some by me. The only difference we have made is a change in paraffin paraffin. Any suggestions for a smoother section? Just for informational purposes, the old paraffin used was Henry?s wonder wax which has been discontinued and the new paraffin is Surgipath EM 400. Thanks, Jennifer Sent from Jenny's iPhone From tbraud at holyredeemer.com Mon Jan 23 09:31:48 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Mon, 23 Jan 2023 15:31:48 +0000 Subject: [Histonet] Bone saw Message-ID: <2a7fff45ee0b4575ac51dd4f68ef6c1b@holyredeemer.com> We have used the IMEB band saw for years and love the results. It has a similar footprint and will cut through anything, including a steel pin. It also has a diamond blade and optional water cooling. It does seem a little awkward to clean, and I wish the protective housing was a little better. If our IMEB failed, I would certainly take a long hard look at the Exakt 302 because it seems to address some of the cons of the IMEB. I don't know about cost comparison. Best of luck. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Today's Topics: 1. Looking for Bone/Pathology saw recommendations (M.O.) ---------------------------------------------------------------------- Message: 1 Date: Fri, 20 Jan 2023 10:53:59 -0800 From: "M.O." Subject: [Histonet] Looking for Bone/Pathology saw recommendations Hello and Happy Friday! I work in an arthritis research lab and we are looking into pathology saws like the Exakt 302. We study lumbar spine and knees. During our tissue harvesting procedure we take slabs of each vertebral disc unit, facet joints, and knee osteochondral samples. Does anyone have experience in cutting bone specimens with a saw like this? Do you have any recommendations on the brand you prefer? Sincerely, Merissa From TNMayer at mdanderson.org Tue Jan 24 08:38:44 2023 From: TNMayer at mdanderson.org (Mayer,Toysha N) Date: Tue, 24 Jan 2023 14:38:44 +0000 Subject: [Histonet] BLOCKS AND SLIDES Message-ID: Another thing that can be done is to donate them to your closest histology program. If they have a student lab, blocks are always needed. If you facility is a teaching hospital, you may already be covered under the initial waiver for uses for teaching, or whatever it's called. Some programs will pay for shipping, and will re-embed the blocks as well for privacy. Sincerely, Toysha N. Mayer, DHSc, MBA, HT (ASCP) Assistant Professor/Associate Program Director HTL Program School of Health Professions MD Anderson Cancer Center tnmayer at mdanderson.org 713-563-3481 wk 832-710-1837 cell Message: 1 Date: Thu, 12 Jan 2023 18:01:39 +0000 (UTC) From: Dorothy Glass To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] BLOCKS AND SLIDES Message-ID: <2133152761.579610.1673546499433 at mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 What is the required length of time that you must keep blocks and slides in histology ------------------------------ Message: 2 The CAP requirement is a minimum of 10 years for both. Your state's department of health may have different standards. The strictest standard should always be adhered to. Stacy McLaughlin, HT(ASCP) QLScm Histology Supervisor Cooley Dickinson Hospital 30 Locust Street Northampton, MA 01060 Office: (413)582-2019 Lab: (413)582-2179 smclaughlin7 at cooleydickinson.org -----Original Message----- From: Dorothy Glass via Histonet Sent: Thursday, January 12, 2023 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] BLOCKS AND SLIDES External Email - Use Caution What is the required length of time that you must keep blocks and slides in histology ------------------------------ Message: 3 Date: Thu, 12 Jan 2023 18:45:10 +0000 From: "Whitaker, Bonnie" To: Dorothy Glass , "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] BLOCKS AND SLIDES Message-ID: Content-Type: text/plain; charset="us-ascii" It depends on who your most stringent governing body is. If you have a lab in NY, or that takes specimens from NY, it's 20 years for both. For CAP it's 10 years. There may be other states that have more stringent rules, etc. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Dorothy Glass via Histonet Sent: Thursday, January 12, 2023 1:02 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] BLOCKS AND SLIDES External Email: CAUTION What is the required length of time that you must keep blocks and slides in histology _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!AcgyE0lytWhQ93VpOoSaZQdMsM7msm3OiqWcJpTtgOxrg8ezN9A4imEm81YtEO98K2qFpE_RSeeKIOkvuWzaStjryx-KdVumiMnE_g$ ------------------------------ Message: 4 Date: Thu, 12 Jan 2023 20:43:53 +0000 From: Ken M To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Expired Tissue Blocks Message-ID: Content-Type: text/plain; charset="iso-8859-1" Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. ------------------------------ Message: 5 Date: Thu, 12 Jan 2023 21:05:06 +0000 From: "Whitaker, Bonnie" To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Expired Tissue Blocks Message-ID: Content-Type: text/plain; charset="us-ascii" Caution to all--make sure your patients have signed consent forms that will include this use of their tissue. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Ken M via Histonet Sent: Thursday, January 12, 2023 3:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Expired Tissue Blocks External Email: CAUTION Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!AU3bcTlGKuA!G28o9Kc7mldDguiyt-GBNAuODGNByP6jiHkEV_WQcSZkzxznBtLMC1hfOWgZLKZ8W5auSSJzH2-kuOJ4Hzt4Ep4aitSlSJ5LvlUfyw$ ------------------------------ Message: 6 Date: Thu, 12 Jan 2023 21:36:05 +0000 From: Ken M To: "histonet at lists.utsouthwestern.edu" Subject: [Histonet] Tissue blocks Message-ID: Content-Type: text/plain; charset="iso-8859-1" If that were true, all cancer research and antibody development in the USA would come to a complete halt. Get a signature from someone who died of cancer 10 years ago?? Here are the findings of most US courts. Here are the facts. In legal disputes concerning the research use of excised tissues, courts consistently have rejected the idea that patients are the owners of their excised tissues or retain any property rights. While only three cases have been adjudicated to date, the findings (in both state and federal courts) have been consistent: patients do not have individual ownership, retain property rights, or have complete control over research use of their excised tissues.9, Contact your legal Counsil to find the tructh. We do not want any patient identifiers or case numbers. ------------------------------ Message: 7 Date: Thu, 12 Jan 2023 21:54:50 +0000 From: Ken M To: "histonet at lists.utsouthwestern.edu" Subject: Re: [Histonet] Expired Tissue Blocks Message-ID: Content-Type: text/plain; charset="us-ascii" The law is the law in the USA. So far the unanoumous decisions across the USA courts have been very clear. For those who are being super cautious despite the prevailing laws of the USA, You are doing very little to help develop new antibodies and enhance cancer research. ________________________________ From: Whitaker, Bonnie via Histonet Sent: Thursday, January 12, 2023 9:05 PM To: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] Expired Tissue Blocks Caution to all--make sure your patients have signed consent forms that will include this use of their tissue. Bonnie Whitaker Ohio State University AP Operations Director 614-293-8418 -----Original Message----- From: Ken M via Histonet Sent: Thursday, January 12, 2023 3:44 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Expired Tissue Blocks External Email: CAUTION Don't throw your expired embedded blocks in the trash. Some of them can be very valuable for research and antibody development. If you can provide a brief description of any positive block (no patient identifiers or names please), we will pay you top dollar to take them and send you all shipping labels and documents ahead of time so you can send your entire storage unit to us. We can provide certification that we will properly dispose of and be responsible for the blocks in our possession if our research partners find them of no use. For some specific positive tissues, a single block blocks can be worth over a hundred dollars to our partners. Please contact us at contactus at springsidesci.com if interested. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__https://na01.safelinks.protection.outlook.com/?url=http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet&data=05*7C01*7C*7Ccf3618d662ec452bf6fe08daf4e19134*7C84df9e7fe9f640afb435aaaaaaaaaaaa*7C1*7C0*7C638091546887356325*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=npRxLEwmB8vLkgpIKybAzEZs*2BAjfDVTHTHOGJsP8EJQ*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!PfbeBCCAmug!hNY4QgeTcq61wqrExJxtGHslaTraUYSuCE4GnbMsE5iNEWaQ7YnVjzoLJhVD1MoVWwgS6dh6Sc5WZQgi6LfnKkW8LnRan2wnKkSVd1kNfBU$ ------------------------------ Subject: Digest Footer _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!PfbeBCCAmug!hNY4QgeTcq61wqrExJxtGHslaTraUYSuCE4GnbMsE5iNEWaQ7YnVjzoLJhVD1MoVWwgS6dh6Sc5WZQgi6LfnKkW8LnRan2wnKkSV2XcheXw$ ------------------------------ End of Histonet Digest, Vol 230, Issue 4 **************************************** The information contained in this e-mail message may be privileged, confidential, and/or protected from disclosure. This e-mail message may contain protected health information (PHI); dissemination of PHI should comply with applicable federal and state laws. If you are not the intended recipient, or an authorized representative of the intended recipient, any further review, disclosure, use, dissemination, distribution, or copying of this message or any attachment (or the information contained therein) is strictly prohibited. If you think that you have received this e-mail message in error, please notify the sender by return e-mail and delete all references to it and its contents from your systems. From gu.lang at gmx.at Fri Jan 27 11:38:10 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Fri, 27 Jan 2023 18:38:10 +0100 Subject: [Histonet] Alcianblue for fast frozen sections Message-ID: <000301d93276$20bdf530$6239df90$@gmx.at> Dear histonetters! Today I've heard about alcianblue staining on fast frozen sections, but I've got no details. I would like to know, if the staining result is the same as for staining AB on paraffinslides. They use the stain on transplantation liver. Is this a usual procedure? I would be glad about any information. Thanks in advance Gudrun From areilly1 at chsbuffalo.org Fri Jan 27 12:03:48 2023 From: areilly1 at chsbuffalo.org (Reilly, Amie) Date: Fri, 27 Jan 2023 18:03:48 +0000 Subject: [Histonet] Post a question Message-ID: <001b89ff2d604fa7be322979c7671a46@CHEXCH02.ad.wnychs.org> Good Morning, I would like to post a question on histonet: My organization is made up of multiple hospitals and surgery centers. All specimens are couriered to the Pathology Department which is located at only one of the facilities. The sites without a fully operational pathology lab provide frozen section support and are equipped with grossing stations. Currently any below and above knee leg amputations are held at the collecting hospital and a pathologist is deployed to gross the leg at that location. Only the blocks are transported to the pathology department. My question is where do other systems with multiple hospitals and surgery centers, with an offsite pathology department, gross their amputations? Is the entire limb being transported to the offsite Pathology Lab or do personnel gross at collecting location ? If the entire limb is being couriered, how is it packaged? Amie Reilly, MLS (ASCP)CM Pathology Manager Catholic Health System Buffalo, NY 14221 (Phone) 716-828-2440 (Fax) 716-828-2791 From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] Sent: Friday, January 27, 2023 1:00 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 230, Issue 13 Attention: This email came from an external source. Do not open attachments or click on links from unknown senders or unexpected emails. Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." CONFIDENTIALITY NOTICE: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender by reply e-mail, delete this e-mail from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. This message and all contents may be reviewed by authorized parties of the Catholic Health System other than those named in the message header. From cls71877 at gmail.com Fri Jan 27 12:55:47 2023 From: cls71877 at gmail.com (Cristi Rigazio) Date: Fri, 27 Jan 2023 13:55:47 -0500 Subject: [Histonet] Post a question In-Reply-To: <001b89ff2d604fa7be322979c7671a46@CHEXCH02.ad.wnychs.org> References: <001b89ff2d604fa7be322979c7671a46@CHEXCH02.ad.wnychs.org> Message-ID: Good afternoon, We are set up the same way, but we have a pathologist located at each location. We do have a mix of on site and courier services. When we transport to the hub for crossing we use a large cooler. Thanks Cristi > On Jan 27, 2023, at 1:10 PM, Reilly, Amie via Histonet wrote: > > ?Good Morning, > > I would like to post a question on histonet: > > My organization is made up of multiple hospitals and surgery centers. All specimens are couriered to the Pathology Department which is located at only one of the facilities. The sites without a fully operational pathology lab provide frozen section support and are equipped with grossing stations. Currently any below and above knee leg amputations are held at the collecting hospital and a pathologist is deployed to gross the leg at that location. Only the blocks are transported to the pathology department. My question is where do other systems with multiple hospitals and surgery centers, with an offsite pathology department, gross their amputations? Is the entire limb being transported to the offsite Pathology Lab or do personnel gross at collecting location ? If the entire limb is being couriered, how is it packaged? > > > Amie Reilly, MLS (ASCP)CM > Pathology Manager > Catholic Health System > Buffalo, NY 14221 > (Phone) 716-828-2440 > (Fax) 716-828-2791 > > > From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request at lists.utsouthwestern.edu] > Sent: Friday, January 27, 2023 1:00 PM > To: histonet at lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 230, Issue 13 > > Attention: This email came from an external source. Do not open attachments or click on links from unknown senders or unexpected emails. > > > Send Histonet mailing list submissions to > histonet at lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request at lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner at lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > CONFIDENTIALITY NOTICE: This message is confidential, intended only for the named recipient(s) and may contain information that is privileged, or exempt from disclosure under applicable law. > If you are not the intended recipient(s), you are notified that the dissemination, distribution or copying of this message is strictly prohibited. > If you receive this message in error, or are not the named recipient(s), please notify the sender by reply e-mail, delete this e-mail from your computer, and destroy any copies in any form immediately. > Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. > This message and all contents may be reviewed by authorized parties of the Catholic Health System other than those named in the message header. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tony.henwood at health.nsw.gov.au Sun Jan 29 08:00:06 2023 From: tony.henwood at health.nsw.gov.au (Tony Henwood (SCHN)) Date: Sun, 29 Jan 2023 14:00:06 +0000 Subject: [Histonet] Alcianblue for fast frozen sections In-Reply-To: <000301d93276$20bdf530$6239df90$@gmx.at> References: <000301d93276$20bdf530$6239df90$@gmx.at> Message-ID: Hello Gudrun, Alcian blue for fast frozen sections I was not aware that alcian blue would be routinely used on frozen sections of transplant liver biopsies. There are other staining techniques that contain a blue dye, that have been used in liver biopsies including Masson's Trichrome (aniline blue) and Millers Stain (Victoria blue). Sulphated Alcian Blue has been used for the demonstration of amyloid in liver biopsies, though not usually on transplant liver biopsies. Alcian blue has been used on frozen sections to demonstrate carboxylated mucins (J Clin Pathol 42(1): 101-105, 1989). Alcian blue staining has been used on FFPE sections of transplant liver sections to show loss of heparan sulphate from endothelial cells of liver graft vessels. Alcian blue at pH 5.8 with 0.3 M MgCl2 on 3-micrometre paraffin-embedded liver section. At this MgCl2 concentration only sulfate glycosaminoglycans such as heparan sulfate stain. Loss of heparin sulphate reduces the ability of the graft to maintain an anti-coagulant environment and can result in diffuse microvascular thrombosis and vascular congestion (see Remuzzi et al (2005). Hemolytic uremic syndrome: a fatal outcome after kidney and liver transplantation performed to correct factor H gene mutation. American Journal of Transplantation, 5(5), 1146-1150). If this is the alcian blue technique needed, then the question arises whether heparan sulphate is insoluble, and therefore stainable, following cryotomy and fixation. Heparan sulphate has a solubility of 100mg/mL in water but is insoluble in ethanol. I would suggest validating the technique on frozen sections of kidney and liver, comparing the staining results to corresponding FFPE sections. Please tell us how you go. Tony Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children?s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA ________________________________ From: Gudrun Lang via Histonet Sent: 28 January 2023 04:38 To: histonet at lists.utsouthwestern.edu Subject: [Histonet] Alcianblue for fast frozen sections Dear histonetters! Today I've heard about alcianblue staining on fast frozen sections, but I've got no details. I would like to know, if the staining result is the same as for staining AB on paraffinslides. They use the stain on transplantation liver. Is this a usual procedure? I would be glad about any information. Thanks in advance Gudrun _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.