From Jessica.Piche at wtbyhosp.org Thu Feb 2 11:12:45 2023 From: Jessica.Piche at wtbyhosp.org (Piche, Jessica) Date: Thu, 2 Feb 2023 17:12:45 +0000 Subject: [Histonet] CAP regulatory question Message-ID: Good Afternoon, Do we only have to keep lot to lot comparisons for immunohistochemistry and special stains for 2 years? I hope everyone is having a good day. Thank you. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 From bcooper at chla.usc.edu Thu Feb 2 13:16:39 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Thu, 2 Feb 2023 19:16:39 +0000 Subject: [Histonet] CAP regulatory question In-Reply-To: References: Message-ID: <0622698e0674428297c9ab178d07bc39@chla.usc.edu> Hi Jessica, That's the way we read it here, but in most cases we have probably more like 3 years-worth. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu Thanks, Brian -----Original Message----- From: Piche, Jessica via Histonet Sent: Thursday, February 2, 2023 9:13 AM To: Histonet Subject: [Histonet] CAP regulatory question (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Good Afternoon, Do we only have to keep lot to lot comparisons for immunohistochemistry and special stains for 2 years? I hope everyone is having a good day. Thank you. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1fb3nVHO0A6uF-g1wQEaKSJkH7Y5EIrKDGcn-2wVHzYNtyHf1A1MeXxsRdMzhQl4hOuVd1UtJzeP9_Uklw9PalE3nKV33hD27IYLQ8Omlrc6-mWtS231ZSWxdvWgfa2e2hs_ONOuP1aiRT9SDnjXWhHMerGSe49Y0Lkk-5NVA92Gg-WRipR1KO94pUi7uB6amVQuQM9GnJqqlKOSRUZ425yVSle00pGHNEqBJdCRouJTzIj1F6fVGJk_0XaZyRaeLJL8t-IfkIgK9RdLzoSmoI7xosJw3ieg1LjbiGOgxU-MRmTcT4hG43R0e1yXyTxcY7HngSAzHU5wH3JzFFiT0zJBl_A1glXv77LbChqWzjEC4KYzstVTM6Pu8oV0roSa6q2mFgJ481q1UZ5PQLWB_IcT5Er1acnhPsjBokbJbLHfssdRptdz7CAlW_kipt6H7lVD7JGw5xplsdPCgNR5Xtw/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From sbonner at pathregional.com Thu Feb 2 18:21:43 2023 From: sbonner at pathregional.com (Silvia Bonner) Date: Fri, 3 Feb 2023 00:21:43 +0000 Subject: [Histonet] prostate needle bx Message-ID: Hi Everyone, What are the different processor run times for prostate needle biopsies? We might be processing them and I wanted to get an idea of schedules and average turnaround time. Are you doing the same day? Overnight? Thank you, Silvia Bonner, BS, HT(ASCP) CM Histology Supervisor sbonner at pathregional.com Pathologists? Regional Laboratory Anatomical Pathology 2841 Juniper Dr. Suite 1 Lewiston, ID 83501 (208)746-5533 This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. Pathologist's Regional Laboratory From cls71877 at gmail.com Thu Feb 2 19:18:41 2023 From: cls71877 at gmail.com (Cristi Rigazio) Date: Thu, 2 Feb 2023 20:18:41 -0500 Subject: [Histonet] prostate needle bx In-Reply-To: References: Message-ID: <893F0B17-7CDA-4392-B8B7-CFC22AD220C5@gmail.com> Hi Silvia, We process them with the rest of our small stuff. About 3 hours on a Peloris. We get them and process them on an ASAP run to embed cut stain and distribute overnight to be on the pathologists desk at 8. > On Feb 2, 2023, at 7:31 PM, Silvia Bonner via Histonet wrote: > > ?Hi Everyone, > > What are the different processor run times for prostate needle biopsies? We might be processing them and I wanted to get an idea of schedules and average turnaround time. Are you doing the same day? Overnight? > > Thank you, > > > Silvia Bonner, BS, HT(ASCP) CM > > Histology Supervisor > > > > sbonner at pathregional.com > > Pathologists? Regional Laboratory > > Anatomical Pathology > > 2841 Juniper Dr. Suite 1 > > Lewiston, ID 83501 > > (208)746-5533 > > > > This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. > > > > This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. > > Pathologist's Regional Laboratory > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Emmanuel-Geoffre.S.Gonzaga at kp.org Fri Feb 3 08:03:19 2023 From: Emmanuel-Geoffre.S.Gonzaga at kp.org (Emmanuel Geoffrey S. Gonzaga) Date: Fri, 3 Feb 2023 14:03:19 +0000 Subject: [Histonet] prostate needle bx In-Reply-To: <893F0B17-7CDA-4392-B8B7-CFC22AD220C5@gmail.com> References: <893F0B17-7CDA-4392-B8B7-CFC22AD220C5@gmail.com> Message-ID: Hello all, Same here. Thank you, -------- Emmanuel Gonzaga Operations Manager (Histology/IHC) Kaiser Permanente Histology Department- Chino Hills 13000 Peyton Drive Chino Hills, CA 91709 (909) 703-6931 (Office) / 263 (tie-line) (909) 703-6924 (Lab) (909) 703-6080 (Fax) --------- kp.org/thrive Upcoming PTO: -----Original Message----- From: Cristi Rigazio via Histonet Sent: Thursday, February 2, 2023 5:19 PM To: Silvia Bonner Cc: HistoNet Subject: Re: [Histonet] prostate needle bx Caution: This email came from outside Kaiser Permanente. Do not open attachments or click on links if you do not recognize the sender. ______________________________________________________________________ Hi Silvia, We process them with the rest of our small stuff. About 3 hours on a Peloris. We get them and process them on an ASAP run to embed cut stain and distribute overnight to be on the pathologists desk at 8. > On Feb 2, 2023, at 7:31 PM, Silvia Bonner via Histonet wrote: > > ?Hi Everyone, > > What are the different processor run times for prostate needle biopsies? We might be processing them and I wanted to get an idea of schedules and average turnaround time. Are you doing the same day? Overnight? > > Thank you, > > > Silvia Bonner, BS, HT(ASCP) CM > > Histology Supervisor > > > > sbonner at pathregional.com > > Pathologists? Regional Laboratory > > Anatomical Pathology > > 2841 Juniper Dr. Suite 1 > > Lewiston, ID 83501 > > (208)746-5533 > > > > This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. > > > > This email may contain physician, patient and/or attorney material that is confidential or privileged for the sole use of the intended recipient. YOU ARE NOTIFIED THAT ANY REVIEW, RELIANCE, DISSEMINATION, DISTRIBUTION OR COPYING OF THIS COMMUNICATION WITHOUT EXPRESS PERMISSION IS STRICTLY PROHIBITED. If you are not the intended recipient, please notify us immediately by telephone at (208) 746-0516 or (208) 746-5533 and delete all copies. > > Pathologist's Regional Laboratory > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!r0BDDPTc5tunuZFy5CcCbM5WhVPOZFgQMsViBzogjSjIjid74YjIiCoGKxkpo3PnHqN6z4vYiMU50KD768hv2utYxqAP8yH3mT36i8uhoJqz$ _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!BZ50a36bapWJ!r0BDDPTc5tunuZFy5CcCbM5WhVPOZFgQMsViBzogjSjIjid74YjIiCoGKxkpo3PnHqN6z4vYiMU50KD768hv2utYxqAP8yH3mT36i8uhoJqz$ NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you have received this e-mail in error, please notify the sender immediately by reply e-mail and permanently delete this e-mail and any attachments without reading, forwarding or saving them. v.173.295 Thank you. From pslath at gwu.edu Fri Feb 3 13:58:30 2023 From: pslath at gwu.edu (Patricia Latham) Date: Fri, 3 Feb 2023 14:58:30 -0500 Subject: [Histonet] Need part-time histotech Message-ID: The histology core lab at George Washington Med School has an immediate need for a histotech to provide routine histology services for a few hours per week - processing, embedding, cutting and routine stains are required. IHC proficiency is preferred but not necessary. Hours can be flexible. If interested, please send an email to pslath at gwu.edu From craigak12 at gmail.com Fri Feb 3 16:25:44 2023 From: craigak12 at gmail.com (craigak12 at gmail.com) Date: Fri, 3 Feb 2023 15:25:44 -0700 Subject: [Histonet] UNSUBSCRIBE Message-ID: <4f8401d9381e$753ff380$5fbfda80$@gmail.com> From rfisher0126 at msn.com Mon Feb 6 13:08:10 2023 From: rfisher0126 at msn.com (Renee Fisher) Date: Mon, 6 Feb 2023 19:08:10 +0000 Subject: [Histonet] Organoids Message-ID: Does anyone have a protocol for processing organoids for EM? Sent from my iPhone From Nancy.Schmitt at mercyhealth.com Wed Feb 8 11:29:13 2023 From: Nancy.Schmitt at mercyhealth.com (Nancy Schmitt) Date: Wed, 8 Feb 2023 17:29:13 +0000 Subject: [Histonet] destain for IHC Message-ID: Hello- Has anyone had success destaining an H&E and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. From bcooper at chla.usc.edu Wed Feb 8 11:45:33 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 8 Feb 2023 17:45:33 +0000 Subject: [Histonet] destain for IHC In-Reply-To: References: Message-ID: We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: Nancy Schmitt via Histonet Sent: Wednesday, February 8, 2023 9:29:13 AM To: Histonet Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Hello- Has anyone had success destaining an H&E and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From Charles.Bacon at baystatehealth.org Wed Feb 8 12:34:56 2023 From: Charles.Bacon at baystatehealth.org (Bacon, Charles) Date: Wed, 8 Feb 2023 18:34:56 +0000 Subject: [Histonet] destain for IHC In-Reply-To: References: Message-ID: Hi Nancy, We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway so we find this protocol effective. Then we remove the deparaffinization step from our IHC protocol and run the slide. 1% Acid Alcohol 70% Alcohol......99ml HCL...................1ml Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786? Fax: 413-322-4790 Charles.Bacon at baystatehealth.org -----Original Message----- From: Cooper, Brian Sent: Wednesday, February 8, 2023 12:46 PM To: Nancy Schmitt ; Histonet Subject: Re: [Histonet] destain for IHC We used to do this all the time in my old reference lab. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu ________________________________ From: Nancy Schmitt via Histonet Sent: Wednesday, February 8, 2023 9:29:13 AM To: Histonet Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. Hello- Has anyone had success destaining an H&E and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!f6gMMu3epLW9iWatLaXyHemacm0hDOoYOXCaMU5Ijo60vj-bxgHDrpUHZFZGfrf594v9R-jNYLfSmKM-MmAw_V6es7aYOBgKp9eltUXF9cY0L4I$ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. ---------------------------------------------------------------------- Please view our annual report at http://www.bhannualreport.org CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. From rbasilio9 at sbcglobal.net Wed Feb 8 13:42:36 2023 From: rbasilio9 at sbcglobal.net (RENE MORALES) Date: Wed, 8 Feb 2023 11:42:36 -0800 Subject: [Histonet] destain for IHC In-Reply-To: References: Message-ID: Nancy, I would say that it can be done in a very particular situation: i.e.: no more tissue available, and type of IHC stain. If it is a dual stain, pathologist can not obtain the good results. In the lab I worked before, it was done, but this technique was not part of the protocol. Hope it helps, Ren? Sent from my iPhone > On Feb 8, 2023, at 10:35, Bacon, Charles via Histonet wrote: > > ?Hi Nancy, > > We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway so we find this protocol effective. Then we remove the deparaffinization step from our IHC protocol and run the slide. > > 1% Acid Alcohol > 70% Alcohol......99ml > HCL...................1ml > > Chuck Bacon, HTL(ASCP)CM > Supervisor Histology > Baystate Medical Center > 361 Whitney Ave., Holyoke, MA 01040 > Telephone: 413-322-4786 Fax: 413-322-4790 > Charles.Bacon at baystatehealth.org > > -----Original Message----- > From: Cooper, Brian > Sent: Wednesday, February 8, 2023 12:46 PM > To: Nancy Schmitt ; Histonet > Subject: Re: [Histonet] destain for IHC > > We used to do this all the time in my old reference lab. > > > Thanks, > > > > Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor > > Department of Pathology and Laboratory Medicine > > Children's Hospital Los Angeles > > 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 > > Ph: 323.361.3357 > > bcooper at chla.usc.edu > > ________________________________ > From: Nancy Schmitt via Histonet > Sent: Wednesday, February 8, 2023 9:29:13 AM > To: Histonet > Subject: [Histonet] destain for IHC (EXTERNAL EMAIL) > > ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. > > Hello- > > Has anyone had success destaining an H&E and then running IHC on the same slide? > > Thanks! > > Nancy > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > https://urldefense.com/v3/__http://secure-web.cisco.com/18_6RdTZTUgn6UmJHW1nn3zKxUTmGLFAErCLSQhQ4ue4GO5-KLRwTACOn7-7a4Er6_TzsYZZ4UJExAdC7pqHx-oxEawdx8S5CkePiIsj96I7voATRTAboDo7E2qiK9afrwIVXNNuBtZ8SbVV19aaqLNv_C6W09X2oFytd1wMK-vTSuvU_07Lyhi7dgDV_cJFs_J64GiPgdhlO7IfatGhbbblWSNlWIzmpleM9CYPNaXdR-M97emBmUM5kzsc1I7oLqfd8_UkGkyx7mm3fglIjsE-lgwHGa4s8mnu-E2FVrzwZy--we--SKanQ_Ey8mdl4n7ztSRvyeZBx4VChcrr6mD8SvTxjQrDW6C4Az7JEUIYVsLyZRIiBGlYk5KptoQ5LaF68M_Cgw5-SK01KtqhBCoxSljHTzsEdEkA4-SxEHMJWcIpEMD1w6cBoLG0RGerMMY9vRHwAIHAv-xNMXT30yQ/http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet__;JSUlJSUl!!P_vj-BUkwjF5!f6gMMu3epLW9iWatLaXyHemacm0hDOoYOXCaMU5Ijo60vj-bxgHDrpUHZFZGfrf594v9R-jNYLfSmKM-MmAw_V6es7aYOBgKp9eltUXF9cY0L4I$ > > CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. > > > ---------------------------------------------------------------------- > Please view our annual report at http://www.bhannualreport.org > > > CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please reply to the sender immediately or by telephone at 413-794-0000 and destroy all copies of this communication and any attachments. For further information regarding Baystate Health's privacy policy, please visit our Internet site at https://www.baystatehealth.org. > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang at gmx.at Thu Feb 9 02:20:35 2023 From: gu.lang at gmx.at (Gudrun Lang) Date: Thu, 9 Feb 2023 09:20:35 +0100 Subject: [Histonet] destain for IHC In-Reply-To: References: Message-ID: <000c01d93c5f$634f2070$29ed6150$@gmx.at> We do restaining for IHC also, but without destaining at all. Hematoxylin doesn't matter because nuclei are stained after IHC with hematoxylin anyway. And Eosin gets lost through all the washing in buffer. The problem is, that HE section are usually mounted on non-adhesive slides, and they float away during the procedure. Gudrun -----Urspr?ngliche Nachricht----- Von: Nancy Schmitt via Histonet [mailto:histonet at lists.utsouthwestern.edu] Gesendet: Mittwoch, 8. Februar 2023 18:29 An: Histonet Betreff: [Histonet] destain for IHC Hello- Has anyone had success destaining an H&E and then running IHC on the same slide? Thanks! Nancy Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From greg.dobbin at gmail.com Thu Feb 9 08:05:58 2023 From: greg.dobbin at gmail.com (Greg Dobbin) Date: Thu, 9 Feb 2023 10:05:58 -0400 Subject: [Histonet] destain for IHC Message-ID: Hi Nancy, You should have no problem doing so. If you want proof, just run a couple of your IHC control sections through the H&E stainer as you would for a patient specimen (so right through to coverslipping) and then start the process of removing coverslip, hydration, destain in acid alcohol and then run slides on your IHC platform as you would -without the dewax step obviously. :-) *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *If God made a drink better than whiskey he kept it for himself! * From Maria.Katleba at foundationhealth.org Thu Feb 9 08:38:48 2023 From: Maria.Katleba at foundationhealth.org (Katleba, Maria) Date: Thu, 9 Feb 2023 14:38:48 +0000 Subject: [Histonet] destain for IHC Message-ID: <1B328B9C-4CA5-47F0-9D9C-4AE1C76E0F4F@foundationhealth.org> Oh no!!! We cut all slides on + BUT you can do this. It?s what we do when we have H&E on plain slides. Whether you have a dry unstain from another lab that sent u a slide, or your H&E that you just took the coverslip off? Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft degree oven for 60 min. You want the bottom of the jar to be moist not floating in it After 60 min remove and open UNDER A HOOD (it?s a vaporized carcinogen) Air out 10-15 min? place on stainer The drops vaporize and the aldehyde goes through tissue and make it adhere to the slide permanently. I do this when I cut nails or anything that won?t stay on a plus slide. Maria Sent from my iPhone > On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet wrote: > From tonyreilly55 at gmail.com Fri Feb 10 03:39:59 2023 From: tonyreilly55 at gmail.com (Tony Reilly) Date: Fri, 10 Feb 2023 20:39:59 +1100 Subject: [Histonet] destain for IHC Message-ID: Hi If you are using the peroxidase method the hydrogen peroxide step will remove the eosin and some of the haematoxylin. No need to do a decolorisation step before staining. As Gudren said the only problem may be not having coated slides. Good luck. Sadly retired histologist Tony Sent from my iPhone > On 10 Feb 2023, at 6:07 am, Katleba, Maria via Histonet wrote: > > ?Oh no!!! We cut all slides on + > > BUT you can do this. It?s what we do when we have H&E on plain slides. > > Whether you have a dry unstain from another lab that sent u a slide, or your H&E that you just took the coverslip off? > > Place it in. Coplin jar with 1-3 DROPS of formalin, cover, place In 60ft degree oven for 60 min. You want the bottom of the jar to be moist not floating in it > > After 60 min remove and open UNDER A HOOD (it?s a vaporized carcinogen) > Air out 10-15 min? place on stainer > > The drops vaporize and the aldehyde goes through tissue and make it adhere to the slide permanently. > > I do this when I cut nails or anything that won?t stay on a plus slide. > > Maria > > Sent from my iPhone > >> On Feb 8, 2023, at 11:21 PM, Gudrun Lang via Histonet wrote: >> > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From criley at udel.edu Tue Feb 14 13:59:59 2023 From: criley at udel.edu (Charles Riley) Date: Tue, 14 Feb 2023 14:59:59 -0500 Subject: [Histonet] Antibody control blocks or slides Message-ID: Hello all, I am working on a research project and I need to obtain control tissues for the following antibodies for mouse/rat samples. IL-6 Collagen I CD68 MMP9 CTGF If you have a vendor that you recommend for both positive and negative tissue controls it would be greatly appreciated. From Kelly.Pairan at nationwidechildrens.org Wed Feb 15 11:45:59 2023 From: Kelly.Pairan at nationwidechildrens.org (Pairan, Kelly) Date: Wed, 15 Feb 2023 17:45:59 +0000 Subject: [Histonet] HSV positive Tissue Message-ID: Good Afternoon Histonet Friends! We are currently validating our HSV 1 and 2 and could us a few known positive cases to use. We haven't had a positive HSV patient here in a quite a few years. If you have a few slides you could share, please let me know. I will send you a FedEx way bill. Thanks, Kelly Kelly Pairan, BS, HT (ASCP)CM, QIHCCM (ASCP) Technical Specialist -Anatomic Pathology Department of Pathology and Laboratory Medicine Email: kelly.pairan at nationwidechildrens.org ph: 614-722-5414 fx: 614-722-3033 From doolee at shands.ufl.edu Wed Feb 15 12:36:52 2023 From: doolee at shands.ufl.edu (Dooley, Elaine O.) Date: Wed, 15 Feb 2023 18:36:52 +0000 Subject: [Histonet] IHC position in Gainesville FL Message-ID: <9a8bd5c7ef28484f8e032062746c31e4@shands.ufl.edu> Hi Histonetters, Just wanted to let everyone know I am looking for an histotechnologist to work in the IHC area of the Histology lab here at UF Health Shands hospital. I am looking for an a person that is energetic, hardworking and willing to learn all aspects of the immunohistochemistry area. The Gainesville area has a lot to offer being a college town. Also there is world class springs for scuba diving, and rivers for kayacking and many nature trails. The coast is only a couple of hour to either side if the beach is your thing. Please check out our posting https://shands.careers.ufhealth.org/jobs/Histologist_Histo/Surgical_/Gainesville_Florida/100/314081/ If you want to talk about the position with me personally call 352-265-0111 ext 72117 Elaine Dooley IHC lead tech From modz9636 at gmail.com Tue Feb 21 13:50:58 2023 From: modz9636 at gmail.com (M.O.) Date: Tue, 21 Feb 2023 11:50:58 -0800 Subject: [Histonet] HepB positive tissue - question about safety Message-ID: Hello, We have some donor tissue that showed up positive for HepB on the Ultrio Elite PCR test and Core Ab test, but was negative for the antigen test. We have the samples in zinc buffered formalin. When processing this for FFPE, is there a risk when working with this tissue for potential infection to the histologist if there is an injury during sectioning? Thank you, Merissa From criley at udel.edu Tue Feb 21 14:46:52 2023 From: criley at udel.edu (Charles Riley) Date: Tue, 21 Feb 2023 15:46:52 -0500 Subject: [Histonet] research questions Message-ID: When performing IHC's for research projects is it recommended to use the same species for control tissues or will any tissues that the antibody has been validated for by the company be acceptable? From mills42 at gmail.com Tue Feb 21 15:43:07 2023 From: mills42 at gmail.com (Caroline Miller) Date: Tue, 21 Feb 2023 13:43:07 -0800 Subject: [Histonet] research questions In-Reply-To: References: Message-ID: I would say it depends on the project. I would always strive to get controls from the same species, but sometimes that is just not possible. If the marker's cellular or tissue location is easily recognizable then Ok with the different species, but if it was a diffuse and hard to interpret marker then I would try harder to get an in-species control. I don't think I would use a different species control to determine ideal concentration / protocol for the main cohort. I would get it going on the control and run the cohort and tweak from there with positive samples (hopefully) in the cohort. Length of fixation/processing/age of slides of the control vs cohort is also something to think about. yours, Caroline On Tue, Feb 21, 2023 at 12:53 PM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > When performing IHC's for research projects is it recommended to use the > same species for control tissues or will any tissues that the antibody has > been validated for by the company be acceptable? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Caroline Miller, M.Sc Histology, Imaging, and Image Analysis Specialist 415 2187297 https://www.linkedin.com/in/mills42/ From SThompson4 at sonichealthcareusa.com Tue Feb 21 15:54:15 2023 From: SThompson4 at sonichealthcareusa.com (Stephanie L. Thompson) Date: Tue, 21 Feb 2023 21:54:15 +0000 Subject: [Histonet] Histotech opportunities Message-ID: <7e553c58d0fb4181abc607840748c3b5@sonichealthcareusa.com> Sonic Healthcare USA has a great opportunity located in Somerset, Massachusetts. We are willing to pay relocation for the right candidate. The labs is great and the people are very family oriented. Check out our website at www.sonichealthcareusa.com Apply through the link below: https://shusa.wd5.myworkdayjobs.com/External/job/Somerset-MA/Histotechnologist_REQ-018151 You can also contact me directly at 210-428-1646 or sthompson4 at sonichealthcareusa.com Thank you - Stephanie Thompson This message contains privileged and confidential information intended only for the use of the addressee named above. If you are not the intended recipient of this message you must not disseminate, copy, or take any action in reliance on it. From nmargaryan88 at gmail.com Wed Feb 22 08:16:24 2023 From: nmargaryan88 at gmail.com (Naira Margaryan) Date: Wed, 22 Feb 2023 08:16:24 -0600 Subject: [Histonet] research questions In-Reply-To: References: Message-ID: Hi Charles, I always ran serial sections for negative control - so, yes, the same species. Good luck, Naira On Tue, Feb 21, 2023 at 2:58 PM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > When performing IHC's for research projects is it recommended to use the > same species for control tissues or will any tissues that the antibody has > been validated for by the company be acceptable? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From craigak12 at gmail.com Wed Feb 22 08:26:23 2023 From: craigak12 at gmail.com (craigak12 at gmail.com) Date: Wed, 22 Feb 2023 07:26:23 -0700 Subject: [Histonet] UNSUBSCRIBE In-Reply-To: References: Message-ID: <0df801d946c9$a442eac0$ecc8c040$@gmail.com> Unsubscribe me -----Original Message----- From: Naira Margaryan via Histonet Sent: Wednesday, February 22, 2023 7:16 AM To: Charles Riley Cc: histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] research questions Hi Charles, I always ran serial sections for negative control - so, yes, the same species. Good luck, Naira On Tue, Feb 21, 2023 at 2:58 PM Charles Riley via Histonet < histonet at lists.utsouthwestern.edu> wrote: > When performing IHC's for research projects is it recommended to use > the same species for control tissues or will any tissues that the > antibody has been validated for by the company be acceptable? > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkara at wisehealthsystem.com Wed Feb 22 13:04:41 2023 From: pkara at wisehealthsystem.com (Kara, Phillip) Date: Wed, 22 Feb 2023 19:04:41 +0000 Subject: [Histonet] Special Stainers Message-ID: So I am working for a brand new lab that has been open since September. We currently perform all our specials by hand (roughly 7 stains; 3 silvers) and our numbers are starting to shoot up big time on them. Any recommendations on special stainers and kits that go with them? We are a Sakura flag ship lab but we are open to other equipment if the cost is good and the results are great. Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC 609 Medical Center Dr. Decatur TX. 76234 Office: 940-626-0120 pkara at wisehealthsystems.com [https://attachments.office.net/owa/pkara%40wisehealthsystem.com/service.svc/s/GetAttachmentThumbnail?id=AAMkAGY1MTI5ZDNjLWU3NTgtNDA1NS1hM2NhLWQxODc1MDQ0MmU5MQBGAAAAAAAG%2BU6a0gKKTaEnWb7ih%2BoQBwBaK5n5dSnaQLPfKtCbo5IBAAAAAAEJAABaK5n5dSnaQLPfKtCbo5IBAABLgFJuAAABEgAQAPGmugMa86BHnNf4tfiY61Q%3D&thumbnailType=2&token=eyJhbGciOiJSUzI1NiIsImtpZCI6IkQ4OThGN0RDMjk2ODQ1MDk1RUUwREZGQ0MzODBBOTM5NjUwNDNFNjQiLCJ0eXAiOiJKV1QiLCJ4NXQiOiIySmozM0Nsb1JRbGU0Tl84dzRDcE9XVUVQbVEifQ.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.WhZkvba9Eq838pqyvXzeL70kAivoZmreu0WYNt3iqB8_1-oez56A6zLQULuJr9o9ypmQQssj9deKbLQQ2PP8s_Hw_QJfoikCDf7h_Ho_oRqJ131emfs9uJCIYbfojGn7SFR73iQr8VIuzVh8shs_DmByzi2fjKxwuQIRW6EzJE-eearAUxk2oDqfeWdKyFCj-BkXeZ8FPUglxXKU-bZU0rhAryXjojYfxd7AzEU3jJuuEU5lF1Ltpl5EfLqQLlvkwHgYIChWaWisXX5QFhJ1mM2TLUWJHiJQ-wjE5QCAtz1b99kVtgXRohYA_rIJCSW8sb853mxM74YxwohVQoMfew&X-OWA-CANARY=RiiZcTdBCE2TzgqeELpeDjALI_oGFdsYiCPzjgXJu5RsBG9ZQwnwt2G0DkaG_9YPlYKQYM3yp5g.&owa=outlook.office.com&scriptVer=20230210007.07&animation=true] CONFIDENTIALITY NOTICE: This email is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. This communication may also contain material protected and governed by the Health insurance Portability and Accountability Act of 1996 (HIPAA). This e-mail is only for the personal and confidential use of the individuals to which it is addressed and contains confidential information. If you are not the intended recipient, you are notified that you have received this document in error, and that any reading, distributing, copying or disclosure is unauthorized. If you have received this communication in error, please notify the sender immediately by the telephone number above and destroy the message From bcooper at chla.usc.edu Wed Feb 22 13:09:24 2023 From: bcooper at chla.usc.edu (Cooper, Brian) Date: Wed, 22 Feb 2023 19:09:24 +0000 Subject: [Histonet] Special Stainers In-Reply-To: References: Message-ID: Agilent-Artisan stainers are amazing. You can search old Histonet posts with my explanation as to why! I once had a lot to say about this. Thanks, Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 Ph: 323.361.3357 bcooper at chla.usc.edu -----Original Message----- From: Kara, Phillip via Histonet Sent: Wednesday, February 22, 2023 11:05 AM To: Eddie Martin via Histonet Subject: [Histonet] Special Stainers (EXTERNAL EMAIL) ****CAUTION: BE CAREFUL WITH THIS MESSAGE***** This email came from outside CHLA. Do not open attachments, click on links, or respond unless you expected this message and recognize the email address: histonet-bounces at lists.utsouthwestern.edu. So I am working for a brand new lab that has been open since September. We currently perform all our specials by hand (roughly 7 stains; 3 silvers) and our numbers are starting to shoot up big time on them. Any recommendations on special stainers and kits that go with them? We are a Sakura flag ship lab but we are open to other equipment if the cost is good and the results are great. Phillip Kara BS, HTL(ASCP) : Lead Histology Tech Wise Pathology LLC 609 Medical Center Dr. Decatur TX. 76234 Office: 940-626-0120 pkara at wisehealthsystems.com [https://secure-web.cisco.com/1W2I8ZnRRyP53h6sYjsyA3KJtvbAABXQ_56wZrZBYbUNfmm5-C1x3Sqw0olIUR-tu8gxIsc7gCpI5WJaJ8y_D5z6FQKe3-g3_B-GzjeHOoAzFk-K9wHim2il_295OsAPxtygC_U2rIWpMEAC7_3LPdZsT9nKOGCP1Un-3w6JeSPRNRoecSHRzbV0EfFudWlHHb-0-stMEqbkjfzHt1h5X8sQbJU1W8RTxUJbe_w3T0dyUUB8Fo8HNBkCk079A_C4A38Q8ZlR78FLt-4td2_sfXA/https%3A%2F%2Fattachments.office.net%2Fowa%2Fpkara%2540wisehealthsystem.com%2Fservice.svc%2Fs%2FGetAttachmentThumbnail%3Fid%3DAAMkAGY1MTI5ZDNjLWU3NTgtNDA1NS1hM2NhLWQxODc1MDQ0MmU5MQBGAAAAAAAG%252BU6a0gKKTaEnWb7ih%252BoQBwBaK5n5dSnaQLPfKtCbo5IBAAAAAAEJAABaK5n5dSnaQLPfKtCbo5IBAABLgFJuAAABEgAQAPGmugMa86BHnNf4tfiY61Q%253D%26thumbnailType%3D2%26token%3DeyJhbGciOiJSUzI1NiIsImtpZCI6IkQ4OThGN0RDMjk2ODQ1MDk1RUUwREZGQ0MzODBBOTM5NjUwNDNFNjQiLCJ0eXAiOiJKV1QiLCJ4NXQiOiIySmozM0Nsb1JRbGU0Tl84dzRDcE9XVUVQbVEifQ.eyJvcmlnaW4iOiJodHRwczovL291dGxvb2sub2ZmaWNlLmNvbSIsInVjIjoiM2I0MmFjODRiMTVmNGMyMjkyOTk3NThhYzcwNzE5YjYiLCJzaWduaW5fc3RhdGUiOiJbXCJkdmNfbW5nZFwiLFwiZHZjX2RtamRcIixcImlua25vd25udHdrXCIsXCJrbXNpXCJdIiwidmVyIjoiRXhjaGFuZ2UuQ2FsbGJhY2suVjEiLCJhcHBjdHhzZW5kZXIiOiJPd2FEb3dubG9hZEA0MGM5NTkxYS05M2FjLTQwNTUtOWU3NS1lNjdjYTNlZDRkYjQiLCJpc3NyaW5nIjoiV1ciLCJhcHBjdHgiOiJ7XCJtc2V4Y2hwcm90XCI6XCJvd2FcIixcInB1aWRcIjpcIjExNTM4MDExMjI5NzYyNjg2NTdcIixcInNjb3BlXCI6XCJPd2FEb3dubG9hZFwiLFwib2lkXCI6XCJhNjAwYmQ3MC02N2I3LTRiN2EtYTc0Yi1iMDZkN2VlNGIyYWZcIixcInByaW1hcnlzaWRcIjpcIlMtMS01LTIxLTM5MjgzMzIyMDktMzgyNDU3MTAyMy0zMDU1OTc1OTk1LTEyNTY5MzIxXCJ9IiwibmJmIjoxNjc3MDY3MTAzLCJleHAiOjE2NzcwNjc3MDMsImlzcyI6IjAwMDAwMDAyLTAwMDAtMGZmMS1jZTAwLTAwMDAwMDAwMDAwMEA0MGM5NTkxYS05M2FjLTQwNTUtOWU3NS1lNjdjYTNlZDRkYjQiLCJhdWQiOiIwMDAwMDAwMi0wMDAwLTBmZjEtY2UwMC0wMDAwMDAwMDAwMDAvYXR0YWNobWVudHMub2ZmaWNlLm5ldEA0MGM5NTkxYS05M2FjLTQwNTUtOWU3NS1lNjdjYTNlZDRkYjQiLCJoYXBwIjoib3dhIn0.WhZkvba9Eq838pqyvXzeL70kAivoZmreu0WYNt3iqB8_1-oez56A6zLQULuJr9o9ypmQQssj9deKbLQQ2PP8s_Hw_QJfoikCDf7h_Ho_oRqJ131emfs9uJCIYbfojGn7SFR73iQr8VIuzVh8shs_DmByzi2fjKxwuQIRW6EzJE-eearAUxk2oDqfeWdKyFCj-BkXeZ8FPUglxXKU-bZU0rhAryXjojYfxd7AzEU3jJuuEU5lF1Ltpl5EfLqQLlvkwHgYIChWaWisXX5QFhJ1mM2TLUWJHiJQ-wjE5QCAtz1b99kVtgXRohYA_rIJCSW8sb853mxM74YxwohVQoMfew%26X-OWA-CANARY%3DRiiZcTdBCE2TzgqeELpeDjALI_oGFdsYiCPzjgXJu5RsBG9ZQwnwt2G0DkaG_9YPlYKQYM3yp5g.%26owa%3Doutlook.office.com%26scriptVer%3D20230210007.07%26animation%3Dtrue CONFIDENTIALITY NOTICE: This email is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521 and is legally privileged. This communication may also contain material protected and governed by the Health insurance Portability and Accountability Act of 1996 (HIPAA). This e-mail is only for the personal and confidential use of the individuals to which it is addressed and contains confidential information. If you are not the intended recipient, you are notified that you have received this document in error, and that any reading, distributing, copying or disclosure is unauthorized. If you have received this communication in error, please notify the sender immediately by the telephone number above and destroy the message _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://secure-web.cisco.com/1RC2m7IhavI1TjTTDJ7Orzxm4-5w2OR17lVwoEjed-uqwNqSdXCxizFUn3LF34svAgEDNZyjYSD0gbngtEmXHIqvvrIRswcpCCcZoPDf63NS6mCa-YHL28ZBkeferXboiQm7R8sx6mYwKhV7OTUohcCNZEVavDLOyjOd5xR1BREzqDQ0-2UsgiVez70BSGdxBBDjRtpSSCaJQSL_zXcVqfP5xPoAeLZchpUiuf4p6hX21u6pjsBNXspCcNijuE19b2Zw9vvPmKqcrOXp5nG0NHMdCkh82rP5HiUs1D9JjYcKCuacc1dSrFRD2G0xihPW12oANv5epATp5qdrlnzd3h7w4ea6rTlSJkS9NlOmymJA3FH4vH9pUMGXskt-eHYx66cCl4HcQNrw12sk1s1d22UKqq2o-mzaxrPXw8fZHSCjgT-dN6k02Q-qLe5c3REjE/http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. From gordon at 10db.co.uk Thu Feb 23 04:00:18 2023 From: gordon at 10db.co.uk (Gordon Brown) Date: Thu, 23 Feb 2023 10:00:18 +0000 Subject: [Histonet] Shandon Elliott Autosharp consumables Message-ID: <9c578f29-2767-d085-4e82-b47c4c0c9277@10db.co.uk> I haven't posted anything on here for a while, hope someone can help me as I'm drawing a blank searching on the internet. I'm one of the leaders of an amateur microscopy group in the UK, the Anglian Microscopy Group, and one of the subjects we regularly cover is histology where we help members to make thin sections - usually of plant material - stain and mount them. Quite a few of our members own a Cambridge Rocking microtome as in the past they were very popular with amateur microscopists in the UK. Only problem is that they use a fixed blade and replaceable blade holders are expensive and frankly usually poor quality. We were recently give a very good condition Shandon Elliott Autosharp (think it's a Mark 2) with all the blade holders and four glass plates that we want to use for sharpening Cambridge Rocker blades. It also came with one bottle of abrasive powder, presumably aluminium oxide, but with no sensible indication of grain size. I've been trying to get hold of information on which abrasives should be used and I'm hitting a bit of a blank wall, probably because these machines are effectively obsolete in professional circles. In an older paper I've found the following abrasives were recommended; Aloxite 2F, Aloxite Optical 50, Aloxite 1200 and Polishing Alumina 350, however these are no longer in production and the company that made/marketed them in the UK, the Carborundum Company, Manchester, no longer exists. I don't think the numbers refer to grit sizes, especially the Optical 50. Diamond pastes are unlikely to be of any use to us as they require copper plates that we don't have. Is there anyone out there who can provide me with information on which currently produced aluminium oxide abrasives will be suitable for this machine, preferably with the grit size in micrometres so that that I can look for alternatives? If anyone in the UK actually has any old tins of these materials we'd be very interested in acquiring them! I've given the club email address below if anyone wants to contact us directly. Many thanks Gordon anglian.microscopy at gmail.com www.anglianmc.org.uk From criley at udel.edu Thu Feb 23 10:07:11 2023 From: criley at udel.edu (Charles Riley) Date: Thu, 23 Feb 2023 11:07:11 -0500 Subject: [Histonet] Tissue Processors Message-ID: Has anyone used the Donatello 2 tissue processor from Diapath From tbraud at holyredeemer.com Thu Feb 23 12:16:47 2023 From: tbraud at holyredeemer.com (Terri Braud) Date: Thu, 23 Feb 2023 18:16:47 +0000 Subject: [Histonet] A question for my hospital lab peeps Message-ID: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 ????????? Honesty AccouNtability ??? AgiLity ??? CoLlaboration ? CoMpassion From LNormington at uwhealth.org Thu Feb 23 12:55:38 2023 From: LNormington at uwhealth.org (Normington, Lacy) Date: Thu, 23 Feb 2023 18:55:38 +0000 Subject: [Histonet] A question for my hospital lab peeps In-Reply-To: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> References: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> Message-ID: Hello Terri, Please see an excerpt from our frozen section manual. For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e. opposing surfaces should not be embedded in the same direction. How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level. How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level. Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E. Thanks Lacy Normington Lacy Normington, HTL(CM) She/Her/Hers Manager, Clinical Laboratories Surgical Pathology, Autopsy 600 Highland Ave, Mail 3224 Madison, WI 53792 Office: 608.890.9373 -----Original Message----- From: Terri Braud via Histonet Sent: Thursday, February 23, 2023 12:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] A question for my hospital lab peeps WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://nam04.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7CLnormington%40uwhealth.org%7C39c802fc6114419b6f1508db15ca3212%7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1%7C0%7C0%7C638127730396655742%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=NneLV1tWvVwR7RTukOLBHO4QRmy0EVgPKIg2OzIbAbs%3D&reserved=0 From Merry.Cone at BSWHealth.org Fri Feb 24 12:27:45 2023 From: Merry.Cone at BSWHealth.org (Cone, Merry A) Date: Fri, 24 Feb 2023 18:27:45 +0000 Subject: [Histonet] A question for my hospital lab peeps In-Reply-To: References: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> Message-ID: Hi Terri! At the hospital I'm currently at, we do the same as Lacy's lab for permanent and frozen sections. We also wait for the pathologists to put in IHC requests before cutting them and they will also often request a follow up H&E. I have worked at another facility that would a 'level 1' and 'level 100' on all sentinel nodes without any IHC up front. Hope that helps! Amber Cone, HTL(CM) -----Original Message----- From: Normington, Lacy Sent: Thursday, February 23, 2023 12:56 PM To: Terri Braud ; histonet at lists.utsouthwestern.edu Subject: Re: [Histonet] A question for my hospital lab peeps Hello Terri, Please see an excerpt from our frozen section manual. For all sentinel lymph nodes and non-sentinel lymph nodes obtained from breast and gynecologic surgeries, entirely submit the lymph node after serially sectioning perpendicular to the long axis at 0.2 cm intervals. It is essential that all unique surfaces be represented, i.e. opposing surfaces should not be embedded in the same direction. How many frozen levels? Seeing as we are serially sectioning the lymph node, we only do a single level. How many permanent section Levels? All remnants are submitted after frozen, again we only perform the microtomy of a single level. Any unstained ffpe sections saved or stained IHC? It is not uncommon for AE1/AE3 to be requested which we then cut the IHC request followed by an additional recut H&E. Thanks Lacy Normington Lacy Normington, HTL(CM) She/Her/Hers Manager, Clinical Laboratories Surgical Pathology, Autopsy 600 Highland Ave, Mail 3224 Madison, WI 53792 Office: 608.890.9373 -----Original Message----- From: Terri Braud via Histonet Sent: Thursday, February 23, 2023 12:17 PM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] A question for my hospital lab peeps WARNING: This email appears to have originated outside of the UW Health email system. DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe. I'd love to hear your input On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? How many frozen levels? How many permanent section Levels? Any unstained ffpe sections saved or stained IHC? I'm just trying to make sure that what we're doing is the norm. Thanks in advance. Terri L. Braud, HT(ASCP) HNL Laboratories for Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3689 Fax: 215-938-2021 Honesty AccouNtability AgiLity CoLlaboration CoMpassion _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu https://urldefense.com/v3/__https://nam04.safelinks.protection.outlook.com/?url=http*3A*2F*2Flists.utsouthwestern.edu*2Fmailman*2Flistinfo*2Fhistonet&data=05*7C01*7CLnormington*40uwhealth.org*7C39c802fc6114419b6f1508db15ca3212*7C0fd7902a3b4f49b0b1edaaa4d2b4f5f1*7C0*7C0*7C638127730396655742*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=NneLV1tWvVwR7RTukOLBHO4QRmy0EVgPKIg2OzIbAbs*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!JA_k2roV-A!ASPwfj3ZVD46XC_NZmxt5qQW7S_nAt2r3ES2zSsZuduBtr8z1zfsGo8cwEqZpVQAdDa4HwlRdRUHMQLunLjs2K945QJbmSDSz3wvhPnitg$ ********************************************************************** The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health. From samantha.golden at ymail.com Fri Feb 24 15:34:56 2023 From: samantha.golden at ymail.com (Samantha Golden) Date: Fri, 24 Feb 2023 16:34:56 -0500 Subject: [Histonet] A question for my hospital lab peeps In-Reply-To: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> References: <21d0ceefe2d14ac3bb0651dfe76189d1@holyredeemer.com> Message-ID: <1C94727C-51D1-4846-91D7-055EA80D8867@ymail.com> If a SLN is sent for frozen section (boo hiss) we treat as any other frozen and we typically cut two sections. If the pathologist is able to determine the lymph node is positive, we will only cut a permanent H&E once processed; we do not need to cut all the SLN levels. If the tissue appears negative on frozen then we will treat it as a routine SLN; we then cut the permanent H&E and an unstain, trim in about 100 um, cut an H&E and an unstain, trim in about 100 um and cut the final H&E. Hope this makes sense! Samantha Sent from my iPhone > On Feb 23, 2023, at 1:38 PM, Terri Braud via Histonet wrote: > > ?I'd love to hear your input > On breast mastectomy cases with sentinel lymph nodes, what is your cutting protocol for sentinel lymph nodes sent for frozen section? > How many frozen levels? > How many permanent section Levels? > Any unstained ffpe sections saved or stained IHC? > I'm just trying to make sure that what we're doing is the norm. Thanks in advance. > > Terri L. Braud, HT(ASCP) > HNL Laboratories for > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > Ph: 215-938-3689 > Fax: 215-938-2021 > Honesty > AccouNtability > AgiLity > CoLlaboration > CoMpassion > > _______________________________________________ > Histonet mailing list > Histonet at lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From djemge11 at gmail.com Fri Feb 24 16:14:14 2023 From: djemge11 at gmail.com (Donna Emge) Date: Fri, 24 Feb 2023 16:14:14 -0600 Subject: [Histonet] Job Opening in La Grange, IL Message-ID: Hello all, AdventHealth is hiring for Histology Techs for our new Histology Lab in La Grange, IL. We have day and overnight positions currently available. We are a small lab that offers full benefits, and good pay, while working with a great bunch of people. Everyone helps each other. We work hard to encourage team values, open honest communication and a system of continuous quality improvement. We will perform special stains by hand with kits and immunos on the Ventana Benchmark Ultra. Must have a minimum of one year of experience as a Histology Technician with competency in the areas of processing, embedding, and microtomy. Immuno experience and HT or HTL certification is preferred, but not required. We are hiring now! Click on the links below to see the job description, hours and days, submit your resume and apply. Note: *Lab Location is actually LaGrange Hospital, La Grange, IL* Histology Techs, Overnight (Job Number: 23001761) AdventHealth Jobs - Histology Tech, Overnight in Hinsdale, Illinois, United States Histology Tech, Days (Job Number:23001757) AdventHealth Jobs - Histology Tech, Days in Hinsdale, Illinois, United States If questions, contact *Shaunay Duncan(Jenkins)* AdventHealth Talent Acquisition Partner | Talent Acquisition HRSS Shaunay.Duncan <@t> adventhealth.com or contact me directly at *Donna Emge, HT (ASCP)* UChicago Medicine | AdventHealth GL Regional Laboratory Manager | Histology 708-715-5720 Donna.Emge <@t> AdventHealth.com From djemge11 at gmail.com Mon Feb 27 09:39:08 2023 From: djemge11 at gmail.com (Donna Emge) Date: Mon, 27 Feb 2023 09:39:08 -0600 Subject: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? Message-ID: I hope someone here can help. I recently visited a lab that has the lab assistants weigh placentas and document, cut the membrane off in one piece around the placenta, cut the umbilical cord off 1? from the placenta, then weigh again, document the weight again, and add the formalin for the PAs to gross the next day. They consider what the lab assistants are doing as processing, not part of grossing. Is this correct? The PAs and Pathologists would like the lab assistants to do this at our lab. 1. What is the CLIA and CAP actual definition of grossing? Where do I find this? 2. Is what the lab assistants are doing at that lab actually a part of grossing, or are they just processing the placentas by the regulatory definition? Thank you in advance, *Donna Emge, HT (ASCP)* UChicago Medicine | AdventHealth GL Regional Laboratory Manager | Histology Donna.Emge(@)AdventHealth.com From mcokertx at gmail.com Mon Feb 27 17:31:25 2023 From: mcokertx at gmail.com (Michelle Bell) Date: Mon, 27 Feb 2023 23:31:25 +0000 Subject: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? In-Reply-To: References: Message-ID: This is so hard to unpack everything that could be wrong with this situation. The weights the lab assistants take would lead me to the conclusion that this would be part of grossing, because it is reportable. It?s such a slippery slope when you start delegating tasks associated with high complexity testing. If I were the manager, I would reach out to your accrediting agency for guidance and support. ________________________________ From: Donna Emge via Histonet Sent: Monday, February 27, 2023 9:39:08 AM To: histonet at lists.utsouthwestern.edu Subject: [Histonet] What is considered Grossing according to CLIA and CAP? Definition? I hope someone here can help. I recently visited a lab that has the lab assistants weigh placentas and document, cut the membrane off in one piece around the placenta, cut the umbilical cord off 1? from the placenta, then weigh again, document the weight again, and add the formalin for the PAs to gross the next day. They consider what the lab assistants are doing as processing, not part of grossing. Is this correct? The PAs and Pathologists would like the lab assistants to do this at our lab. 1. What is the CLIA and CAP actual definition of grossing? Where do I find this? 2. Is what the lab assistants are doing at that lab actually a part of grossing, or are they just processing the placentas by the regulatory definition? Thank you in advance, *Donna Emge, HT (ASCP)* UChicago Medicine | AdventHealth GL Regional Laboratory Manager | Histology Donna.Emge(@)AdventHealth.com _______________________________________________ Histonet mailing list Histonet at lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From idimenstein at hotmail.com Mon Feb 27 21:23:50 2023 From: idimenstein at hotmail.com (Izak Dimenstein) Date: Tue, 28 Feb 2023 03:23:50 +0000 Subject: [Histonet] Histonet Digest, Vol 231, Issue 14 In-Reply-To: References: Message-ID: Placenta is a serious surgical pathology specimen. Wrong initial processing can damage the result. The described processing in the laboratory reflects complete misunderstanding by the management the grossing procedure. In my book Grossing Technology (Amazon.com), placenta grossing technique occupies pages 138-142. Izak B. Dimenstein, MD, PhD, HT (ASCP) ________________________________ From: histonet-request at lists.utsouthwestern.edu Sent: Monday, February 27, 2023 1:01 PM To: histonet at lists.utsouthwestern.edu Subject: Histonet Digest, Vol 231, Issue 14 Send Histonet mailing list submissions to histonet at lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request at lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner at lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."