[Histonet] Leica H&E 600

Cooper, Brian bcooper at chla.usc.edu
Wed Aug 23 08:52:44 CDT 2023


The Ventana HE600 is an awesome machine. You can't use xylene to remove the coverslips, you need to heat them on a hot plate to soften the glue, and then the slips just come right off.

I used other dip and dunk stainers in the past and I was skeptical at first too. Those concerns have been put to rest! The machine requires no manual user maintenance (PM service is of course required) or filtering of reagents. You can't put expired reagents on the machine, nor can you put reagents in the wrong place. The machine performs a cleaning cycle every evening, so we literally do nothing on this machine except press start.

No machine is completely perfect. Sometimes staining modules leak or fail. This machine has 3 separate staining modules so you can always disable the troubled module and the machine will still work. It's been very rare that our coverslipper has failed.

It's certainly not the cheapest stainer on the market, but I've told people who come to our lab that the machine is like another employee! It just works and works and works. I'll be happy to answer any further questions.


Thanks,



Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor

Department of Pathology and Laboratory Medicine

Children's Hospital Los Angeles

4650 Sunset Blvd MS#43- Los Angeles, CA 90027

Ph: 323.361.3357<tel:+13233613357>

bcooper at chla.usc.edu<mailto:bcooper at chla.usc.edu>

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From: O'Neil, Beth via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, August 23, 2023 5:28:11 AM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Leica H&E 600 (EXTERNAL EMAIL)

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I would appreciate any feedback from anyone with experience using the Leica H&E 600. I recently attended a demo of the unit and we are still hung up on the large slide tray it uses instead of the traditional slide racks.   I also spoke with another user who indicated that if you need to re-coverslip a slide it is very difficult to remove the existing coverslip and when you do the hematoxylin is removed from the stain ???  We already know we will need two units to handle our workload of approximately 900 H&E.  Any information would be greatly appreciated.

Beth O'Neil
beth.oneil1 at wvumedicine.org<mailto:beth.oneil1 at wvumedicine.org>
West Virginia University, JW Ruby Memorial Hospital
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